AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068993

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068993.t1 MTR_8g442410 89.474 513 26 2 1 486 25 536 0.0 942
MS.gene068993.t1 MTR_4g104020 44.961 387 167 7 126 475 143 520 1.62e-98 306
MS.gene068993.t1 MTR_7g027190 41.237 388 182 6 126 475 294 673 3.30e-90 289
MS.gene068993.t1 MTR_3g065980 36.579 380 208 10 126 476 166 541 2.56e-78 254
MS.gene068993.t1 MTR_3g089055 35.676 370 208 7 133 475 204 570 2.07e-66 224
MS.gene068993.t1 MTR_3g089055 35.676 370 208 7 133 475 199 565 2.16e-66 224
MS.gene068993.t1 MTR_2g082090 37.135 377 203 8 128 475 208 579 9.58e-66 222
MS.gene068993.t1 MTR_1g029420 35.040 371 209 8 133 475 226 592 2.43e-65 221
MS.gene068993.t1 MTR_5g094450 33.684 380 216 7 128 475 157 532 7.02e-64 216
MS.gene068993.t1 MTR_5g097480 33.511 376 218 8 128 475 173 544 3.59e-63 214
MS.gene068993.t1 MTR_3g056110 33.777 376 217 9 128 475 171 542 6.93e-62 211
MS.gene068993.t1 MTR_5g009080 30.556 432 219 9 125 476 49 479 5.73e-59 202
MS.gene068993.t1 MTR_7g074650 35.180 361 203 7 136 475 442 792 2.71e-57 203
MS.gene068993.t1 MTR_4g064150 30.024 413 240 8 103 477 334 735 3.29e-57 202
MS.gene068993.t1 MTR_4g064160 30.166 421 251 9 90 477 276 686 1.09e-55 197
MS.gene068993.t1 MTR_4g064180 29.977 427 253 9 85 473 205 623 4.33e-55 194
MS.gene068993.t1 MTR_4g102790 32.292 384 221 9 124 474 284 661 5.61e-55 195
MS.gene068993.t1 MTR_4g064120 30.374 428 248 11 85 472 205 622 1.87e-54 193
MS.gene068993.t1 MTR_4g133660 31.662 379 222 7 127 474 170 542 8.01e-54 189
MS.gene068993.t1 MTR_2g097410 31.053 380 233 5 126 477 365 743 9.58e-54 192
MS.gene068993.t1 MTR_4g064200 30.563 373 232 6 127 474 279 649 3.16e-52 187
MS.gene068993.t1 MTR_2g097467 29.255 376 240 5 126 477 283 656 1.04e-50 183
MS.gene068993.t1 MTR_2g097463 29.600 375 238 5 127 477 285 657 4.24e-50 181
MS.gene068993.t1 MTR_4g076020 34.316 373 198 10 136 483 88 438 5.15e-50 177
MS.gene068993.t1 MTR_7g062120 30.256 390 241 6 116 477 356 742 2.06e-49 181
MS.gene068993.t1 MTR_2g097310 29.793 386 224 10 127 477 267 640 2.95e-49 179
MS.gene068993.t1 MTR_1g069725 33.862 378 207 11 124 474 101 462 6.60e-49 174
MS.gene068993.t1 MTR_2g097473 28.457 376 243 5 126 477 283 656 2.78e-48 176
MS.gene068993.t1 MTR_2g097390 29.793 386 218 10 127 474 270 640 1.39e-47 174
MS.gene068993.t1 MTR_2g026250 29.973 377 233 8 127 475 223 596 3.57e-47 172
MS.gene068993.t1 MTR_2g097380 30.226 354 215 8 137 477 229 563 7.36e-47 171
MS.gene068993.t1 MTR_2g097350 30.233 387 215 12 127 474 269 639 8.19e-47 172
MS.gene068993.t1 MTR_2g034250 30.412 388 228 10 125 475 203 585 2.82e-43 161
MS.gene068993.t1 MTR_3g072710 31.436 404 216 14 124 478 112 503 2.73e-42 157
MS.gene068993.t1 MTR_3g022830 28.162 419 217 9 133 475 26 436 3.64e-42 155
MS.gene068993.t1 MTR_5g058860 31.218 394 226 12 125 478 112 500 6.27e-42 156
MS.gene068993.t1 MTR_2g034280 29.765 383 234 9 125 475 196 575 1.98e-41 156
MS.gene068993.t1 MTR_7g057230 32.416 327 193 7 172 474 334 656 5.05e-41 156
MS.gene068993.t1 MTR_2g034260 29.592 392 225 9 125 475 203 584 1.08e-39 151
MS.gene068993.t1 MTR_5g015490 27.851 377 237 7 133 475 115 490 4.80e-38 145
MS.gene068993.t1 MTR_4g076140 45.270 148 79 1 330 475 322 469 8.39e-38 144
MS.gene068993.t1 MTR_4g076140 33.023 215 115 4 125 317 41 248 1.63e-25 109
MS.gene068993.t1 MTR_1g096030 27.536 414 234 14 113 475 61 459 2.08e-37 143
MS.gene068993.t1 MTR_1g086970 27.970 404 242 12 111 475 85 478 1.06e-36 141
MS.gene068993.t1 MTR_4g097080 27.586 377 232 11 133 475 134 503 2.81e-36 140
MS.gene068993.t1 MTR_4g077760 28.307 378 228 11 129 475 190 555 3.59e-33 132
MS.gene068993.t1 MTR_6g047750 30.504 377 222 12 131 475 294 662 5.29e-33 133
MS.gene068993.t1 MTR_2g089100 26.585 410 243 8 118 474 38 442 9.33e-32 127
MS.gene068993.t1 MTR_4g095500 26.585 410 231 11 133 474 60 467 4.72e-31 125
MS.gene068993.t1 MTR_5g015950 26.781 351 204 12 170 474 102 445 1.04e-29 121
MS.gene068993.t1 MTR_3g053270 26.781 351 204 12 170 474 102 445 1.04e-29 121
MS.gene068993.t1 MTR_2g099110 28.831 385 224 12 133 475 75 451 3.25e-29 120
MS.gene068993.t1 MTR_7g109580 25.849 383 244 12 124 475 181 554 2.94e-26 112
MS.gene068993.t1 MTR_8g093070 25.389 386 244 13 129 485 136 506 1.45e-23 103
MS.gene068993.t1 MTR_0092s0100 23.077 377 243 9 129 475 372 731 6.31e-22 99.8
MS.gene068993.t1 MTR_0092s0100 23.077 377 243 9 129 475 369 728 7.26e-22 99.8
MS.gene068993.t1 MTR_4g026485 21.762 386 254 10 119 475 258 624 3.99e-17 84.7
MS.gene068993.t1 MTR_3g022580 35.115 131 75 4 353 475 55 183 1.27e-15 75.5
MS.gene068993.t1 MTR_8g020840 21.474 312 209 10 190 476 249 549 6.10e-14 74.7
MS.gene068993.t1 MTR_3g022005 37.634 93 55 2 385 475 34 125 9.58e-13 65.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068993.t1 AT1G66350 38.043 368 202 10 126 476 149 507 3.26e-76 248
MS.gene068993.t1 AT2G01570 38.196 377 205 11 126 481 218 587 6.51e-75 247
MS.gene068993.t1 AT1G14920 37.466 371 210 8 126 478 166 532 8.58e-75 245
MS.gene068993.t1 AT3G03450 36.800 375 210 7 126 477 177 547 5.80e-73 241
MS.gene068993.t1 AT5G17490 36.290 372 212 10 126 478 154 519 2.97e-68 228
MS.gene068993.t1 AT5G48150 33.777 376 218 7 128 475 118 490 7.82e-62 209
MS.gene068993.t1 AT5G48150 33.777 376 218 7 128 475 118 490 7.82e-62 209
MS.gene068993.t1 AT5G48150 33.777 376 218 7 128 475 118 490 7.82e-62 209
MS.gene068993.t1 AT5G48150 33.777 376 218 7 128 475 118 490 7.82e-62 209
MS.gene068993.t1 AT2G04890 34.140 372 214 7 128 475 49 413 8.90e-62 207
MS.gene068993.t1 AT3G54220 36.639 363 196 7 136 474 297 649 1.78e-60 210
MS.gene068993.t1 AT1G21450 34.140 372 216 6 131 475 224 593 2.42e-60 208
MS.gene068993.t1 AT1G50600 32.979 376 220 8 128 475 155 526 1.66e-57 199
MS.gene068993.t1 AT1G50600 32.979 376 220 8 128 475 226 597 6.05e-57 199
MS.gene068993.t1 AT1G50600 32.979 376 220 8 128 475 230 601 8.63e-57 199
MS.gene068993.t1 AT5G41920 35.106 376 206 9 126 475 37 400 1.77e-56 193
MS.gene068993.t1 AT1G07530 30.789 380 231 7 128 478 392 768 8.72e-55 196
MS.gene068993.t1 AT2G29060 29.771 393 244 7 112 474 264 654 1.29e-50 183
MS.gene068993.t1 AT4G17230 33.420 386 219 9 121 475 147 525 1.31e-50 181
MS.gene068993.t1 AT4G17230 33.420 386 219 9 121 475 147 525 1.31e-50 181
MS.gene068993.t1 AT2G29060 29.771 393 244 6 112 474 301 691 1.85e-50 183
MS.gene068993.t1 AT2G29065 25.833 480 307 10 36 474 156 627 4.11e-49 178
MS.gene068993.t1 AT5G59450 28.272 382 240 7 127 477 222 600 2.66e-47 173
MS.gene068993.t1 AT1G07520 25.828 453 278 8 56 474 100 528 1.25e-46 170
MS.gene068993.t1 AT1G07520 25.828 453 278 8 56 474 100 528 1.25e-46 170
MS.gene068993.t1 AT1G07520 26.683 401 258 6 108 474 294 692 3.61e-46 171
MS.gene068993.t1 AT1G07520 26.683 401 258 6 108 474 295 693 4.02e-46 171
MS.gene068993.t1 AT1G63100 30.966 352 212 6 123 448 243 589 4.36e-46 170
MS.gene068993.t1 AT1G63100 30.966 352 212 6 123 448 268 614 6.73e-46 170
MS.gene068993.t1 AT3G46600 28.646 384 238 8 124 477 204 581 8.52e-45 166
MS.gene068993.t1 AT1G55580 34.667 300 179 7 190 475 149 445 9.02e-45 163
MS.gene068993.t1 AT2G37650 28.385 384 244 6 122 478 325 704 1.55e-44 166
MS.gene068993.t1 AT3G46600 28.646 384 238 8 124 477 172 549 2.35e-44 164
MS.gene068993.t1 AT2G37650 28.385 384 244 6 122 478 337 716 2.37e-44 166
MS.gene068993.t1 AT3G46600 28.800 375 231 8 133 477 5 373 5.51e-44 159
MS.gene068993.t1 AT3G50650 32.267 344 201 10 160 475 203 542 1.17e-41 156
MS.gene068993.t1 AT5G66770 29.412 374 231 10 129 475 217 584 2.79e-39 150
MS.gene068993.t1 AT4G37650 27.833 406 246 14 114 475 127 529 3.48e-39 149
MS.gene068993.t1 AT4G08250 30.026 383 231 15 124 474 99 476 7.39e-39 147
MS.gene068993.t1 AT5G52510 29.765 383 222 11 131 475 267 640 2.31e-37 145
MS.gene068993.t1 AT1G50420 43.137 153 85 1 325 475 327 479 4.76e-35 137
MS.gene068993.t1 AT1G50420 31.364 220 120 4 125 320 50 262 4.08e-26 111
MS.gene068993.t1 AT3G49950 27.273 385 229 13 133 474 31 407 1.61e-29 120
MS.gene068993.t1 AT3G60630 26.710 307 201 10 183 475 326 622 3.45e-22 100
MS.gene068993.t1 AT4G00150 25.833 360 224 10 137 478 223 557 8.69e-18 86.7
MS.gene068993.t1 AT2G45160 22.846 499 319 19 25 475 159 639 2.64e-15 79.0
MS.gene068993.t1 AT3G13840 24.747 396 233 17 122 475 138 510 3.12e-15 78.6

Find 90 sgRNAs with CRISPR-Local

Find 100 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAATACACATAATTGATT+TGG 0.225391 8.1:-56549815 MS.gene068993:CDS
ATCCTAATTGGACTATTTCT+TGG 0.252505 8.1:+56549328 None:intergenic
TTCCTTTCCAACCAAGTAAA+AGG 0.254662 8.1:+56549089 None:intergenic
CACAATGGACCTTTCTTTCT+TGG 0.260406 8.1:-56549412 MS.gene068993:CDS
TTCAATGGCCTTCGCTAATA+AGG 0.263625 8.1:-56549777 MS.gene068993:CDS
TTTGTTACTTGGTAAAATTT+TGG 0.264110 8.1:-56550097 MS.gene068993:CDS
ACAACACCTTATATTACTTT+TGG 0.300835 8.1:-56549886 MS.gene068993:CDS
TCATGTCTCTCCATTCTATC+AGG 0.340101 8.1:+56549247 None:intergenic
TTGGCTTTGTCAAGTCCTTC+AGG 0.354507 8.1:-56550078 MS.gene068993:CDS
ATGAAACCAAAAGTAATATA+AGG 0.362911 8.1:+56549880 None:intergenic
CCTCCAAAGCTCAGGATAAC+AGG 0.367635 8.1:-56549730 MS.gene068993:CDS
CTGGCTTGTGCTGAGGCCGT+TGG 0.372486 8.1:-56550144 MS.gene068993:CDS
CCTGTTATCCTGAGCTTTGG+AGG 0.384703 8.1:+56549730 None:intergenic
CTGAGCTTTGGAGGCCCCTC+AGG 0.392034 8.1:+56549739 None:intergenic
TCAATACACATAATTGATTT+GGG 0.393109 8.1:-56549814 MS.gene068993:CDS
TGCCAAGAAATAGTCCAATT+AGG 0.408355 8.1:-56549330 MS.gene068993:CDS
TGACTTGGTCCATACGCTGC+TGG 0.410075 8.1:-56550163 MS.gene068993:CDS
ATATACATTGTCATGTGAAA+AGG 0.427162 8.1:-56549116 MS.gene068993:CDS
TCTACAACTGTGAGTGCAAT+TGG 0.437872 8.1:+56549448 None:intergenic
AAGTAATATAAGGTGTTGTT+TGG 0.439313 8.1:+56549890 None:intergenic
AATCCTGTTATCCTGAGCTT+TGG 0.439914 8.1:+56549727 None:intergenic
GTTGAAATGAACGGCAACTT+TGG 0.442663 8.1:-56550231 MS.gene068993:CDS
TTGTCGATTGTAAGAAGAGA+AGG 0.450373 8.1:+56549589 None:intergenic
AAACAGGCGAATTTGTTACT+TGG 0.451830 8.1:-56550108 MS.gene068993:CDS
GATCAATGGAGAAGACAGTT+AGG 0.456219 8.1:-56549217 MS.gene068993:CDS
TGTAGAGGAAGCACTCTCAT+TGG 0.457377 8.1:-56549653 MS.gene068993:CDS
GACATGAAAGAGTAGATCAA+TGG 0.459154 8.1:-56549231 MS.gene068993:CDS
ATGGACCTTTCTTTCTTGGT+AGG 0.464231 8.1:-56549408 MS.gene068993:CDS
GCTATATGCCAAGGATCAAA+AGG 0.466005 8.1:-56549844 MS.gene068993:CDS
GAAAGGAAGGTCTATTATGA+TGG 0.467802 8.1:-56549074 MS.gene068993:CDS
GTAGAGGAAGCACTCTCATT+GGG 0.469254 8.1:-56549652 MS.gene068993:CDS
CAGCACAAGCCAGCAGCGTA+TGG 0.480061 8.1:+56550154 None:intergenic
TCTTCATCCTCCATTGTGGT+TGG 0.481380 8.1:+56550303 None:intergenic
GATTACTAGGGAGAATAAGT+TGG 0.489189 8.1:-56549932 MS.gene068993:intron
CATCATCAAGAACTGTCTCT+TGG 0.496418 8.1:+56550511 None:intergenic
GAATGGAGCATGCTCTTCAA+TGG 0.496563 8.1:-56549792 MS.gene068993:CDS
ACCTTGGTTCATACCGAGAC+TGG 0.497138 8.1:+56550188 None:intergenic
TTGCCACAGCATTGAAGTGT+AGG 0.499786 8.1:-56550026 MS.gene068993:intron
AGAAGACAGTTAGGAAGAGC+AGG 0.504894 8.1:-56549208 MS.gene068993:CDS
CTGATATTATGCGAAATTCA+AGG 0.506362 8.1:+56549623 None:intergenic
TAATAAGGAACCTCGCTTCG+AGG 0.507019 8.1:-56549762 MS.gene068993:CDS
CCGAGACTGGCTTGTGATTG+TGG 0.515572 8.1:+56550201 None:intergenic
GCCCCTCAGGCCTCGAAGCG+AGG 0.518387 8.1:+56549752 None:intergenic
CTTTCTGTTTATGATTGTGA+TGG 0.518538 8.1:-56549139 MS.gene068993:CDS
CTATTATGATGGCTTCTGCT+TGG 0.519258 8.1:-56549063 MS.gene068993:CDS
CTGAGGGGCCTCCAAAGCTC+AGG 0.525835 8.1:-56549738 MS.gene068993:CDS
AGCAATATTTGATTCTCTTG+AGG 0.528353 8.1:-56549359 MS.gene068993:CDS
ACACATAATTGATTTGGGAA+TGG 0.530660 8.1:-56549809 MS.gene068993:CDS
AAGTCATCAACTTCTGAAGA+AGG 0.531009 8.1:+56550444 None:intergenic
TACGCTGCTGGCTTGTGCTG+AGG 0.531958 8.1:-56550151 MS.gene068993:CDS
AGAAACCTACCAAGAAAGAA+AGG 0.532154 8.1:+56549403 None:intergenic
AACCTCGCTTCGAGGCCTGA+GGG 0.533889 8.1:-56549754 MS.gene068993:CDS
ATTCACCTTCTCTCAAGTTG+AGG 0.536949 8.1:+56549566 None:intergenic
TATACATTGTCATGTGAAAA+GGG 0.539789 8.1:-56549115 MS.gene068993:CDS
GTAAGAAGAGAAGGAGTTGT+CGG 0.546160 8.1:+56549598 None:intergenic
CAAGAACTGTCTCTTGGTCA+TGG 0.547218 8.1:+56550517 None:intergenic
AAATTCTTCATCCTCCATTG+TGG 0.547730 8.1:+56550299 None:intergenic
CAATGCTGTGGCAAAGCAGT+AGG 0.548768 8.1:+56550035 None:intergenic
TTTGTTGTCTCTGCAGCCAA+CGG 0.552263 8.1:+56550128 None:intergenic
TGGCTGCAGAGACAACAAAC+AGG 0.552808 8.1:-56550124 MS.gene068993:CDS
TGTTGATAGCTTCATCAACA+TGG 0.554822 8.1:-56550421 MS.gene068993:CDS
TAGCATGAATTTGCTTGTAG+AGG 0.564272 8.1:-56549668 MS.gene068993:CDS
GAACCTCGCTTCGAGGCCTG+AGG 0.569227 8.1:-56549755 MS.gene068993:CDS
AGAAGAAATTTGCAACATTG+TGG 0.571117 8.1:-56549281 MS.gene068993:CDS
TGCAACATTGTGGCTTGTGA+GGG 0.571918 8.1:-56549271 MS.gene068993:CDS
TTGCAACATTGTGGCTTGTG+AGG 0.576539 8.1:-56549272 MS.gene068993:CDS
GAGAAGAATCATCAAAGTCA+AGG 0.576589 8.1:-56550372 MS.gene068993:CDS
TTGTGAGGGTCCTGATAGAA+TGG 0.581071 8.1:-56549257 MS.gene068993:CDS
GCTTGACTTGTACACTTGAG+TGG 0.583171 8.1:+56549172 None:intergenic
GCGAGGTTCCTTATTAGCGA+AGG 0.586588 8.1:+56549769 None:intergenic
CCACAATCACAAGCCAGTCT+CGG 0.590657 8.1:-56550201 MS.gene068993:CDS
ACCTCGCTTCGAGGCCTGAG+GGG 0.592685 8.1:-56549753 MS.gene068993:CDS
TGAAAATTCAACAATCACAA+TGG 0.600279 8.1:-56550268 MS.gene068993:CDS
GATGGCTTCTGCTTGGCAAG+TGG 0.608972 8.1:-56549056 MS.gene068993:CDS
GACAACCTCAACTTGAGAGA+AGG 0.621509 8.1:-56549571 MS.gene068993:CDS
GCAAATGAAGCTATATGCCA+AGG 0.622841 8.1:-56549853 MS.gene068993:CDS
AGAAGGTAATAAACAAGGAG+TGG 0.631583 8.1:+56550461 None:intergenic
GCCAGTCTCGGTATGAACCA+AGG 0.639602 8.1:-56550189 MS.gene068993:CDS
CTTTGTAACGAATCTCCTGA+AGG 0.642692 8.1:+56550063 None:intergenic
GAAGAAGAAGTTGAAATGAA+CGG 0.644919 8.1:-56550240 MS.gene068993:CDS
TAACCTACACTTCAATGCTG+TGG 0.647062 8.1:+56550023 None:intergenic
TATGAACCAAGGTCTTGACT+TGG 0.650330 8.1:-56550178 MS.gene068993:CDS
TATGGACCAAGTCAAGACCT+TGG 0.654792 8.1:+56550172 None:intergenic
TGAATATGATCCAACCACAA+TGG 0.666133 8.1:-56550313 MS.gene068993:CDS
AAATATGTGAAAGAAAGTAG+AGG 0.666936 8.1:-56549511 MS.gene068993:CDS
TCTGAAGAAGGTAATAAACA+AGG 0.670330 8.1:+56550456 None:intergenic
CAAGAAAGAAAGGTCCATTG+TGG 0.670675 8.1:+56549413 None:intergenic
ATATGATCCAACCACAATGG+AGG 0.673525 8.1:-56550310 MS.gene068993:CDS
AGAATGGAACAAGATCAAGA+AGG 0.680580 8.1:-56550546 MS.gene068993:CDS
GAGCAAGATACAAACCACAA+TGG 0.698995 8.1:-56549427 MS.gene068993:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATGAAACCAAAAGTAATATA+AGG + chr8.1:56549690-56549709 None:intergenic 20.0%
!! CTATTTGAAAATACATTATG+TGG + chr8.1:56549579-56549598 None:intergenic 20.0%
!!! TCAATACACATAATTGATTT+GGG - chr8.1:56549753-56549772 MS.gene068993:CDS 20.0%
!!! TTATATTACTTTTGGTTTCA+TGG - chr8.1:56549689-56549708 MS.gene068993:CDS 20.0%
!!! TTTGTTACTTGGTAAAATTT+TGG - chr8.1:56549470-56549489 MS.gene068993:CDS 20.0%
! AAATATGTGAAAGAAAGTAG+AGG - chr8.1:56550056-56550075 MS.gene068993:CDS 25.0%
! ATATACATTGTCATGTGAAA+AGG - chr8.1:56550451-56550470 MS.gene068993:CDS 25.0%
! ATGATGTTTCTTTGATTACT+AGG - chr8.1:56549622-56549641 MS.gene068993:CDS 25.0%
! CTCAATACACATAATTGATT+TGG - chr8.1:56549752-56549771 MS.gene068993:CDS 25.0%
! TATACATTGTCATGTGAAAA+GGG - chr8.1:56550452-56550471 MS.gene068993:CDS 25.0%
! TGAAAATTCAACAATCACAA+TGG - chr8.1:56549299-56549318 MS.gene068993:CDS 25.0%
! TGATGTTTCTTTGATTACTA+GGG - chr8.1:56549623-56549642 MS.gene068993:CDS 25.0%
!! ACAACACCTTATATTACTTT+TGG - chr8.1:56549681-56549700 MS.gene068993:CDS 25.0%
!!! AAGTAATATAAGGTGTTGTT+TGG + chr8.1:56549680-56549699 None:intergenic 25.0%
AGAAGAAATTTGCAACATTG+TGG - chr8.1:56550286-56550305 MS.gene068993:CDS 30.0%
AGCAATATTTGATTCTCTTG+AGG - chr8.1:56550208-56550227 MS.gene068993:CDS 30.0%
CTGATATTATGCGAAATTCA+AGG + chr8.1:56549947-56549966 None:intergenic 30.0%
CTTTCTGTTTATGATTGTGA+TGG - chr8.1:56550428-56550447 MS.gene068993:CDS 30.0%
GAAGAAGAAGTTGAAATGAA+CGG - chr8.1:56549327-56549346 MS.gene068993:CDS 30.0%
TCTGAAGAAGGTAATAAACA+AGG + chr8.1:56549114-56549133 None:intergenic 30.0%
! ATCCTAATTGGACTATTTCT+TGG + chr8.1:56550242-56550261 None:intergenic 30.0%
! CTCTCTATTTTCATCCTAAT+TGG + chr8.1:56550254-56550273 None:intergenic 30.0%
!! ACACATAATTGATTTGGGAA+TGG - chr8.1:56549758-56549777 MS.gene068993:CDS 30.0%
AAATTCTTCATCCTCCATTG+TGG + chr8.1:56549271-56549290 None:intergenic 35.0%
AGAAACCTACCAAGAAAGAA+AGG + chr8.1:56550167-56550186 None:intergenic 35.0%
AGAAGGTAATAAACAAGGAG+TGG + chr8.1:56549109-56549128 None:intergenic 35.0%
GAAAGGAAGGTCTATTATGA+TGG - chr8.1:56550493-56550512 MS.gene068993:CDS 35.0%
GACATGAAAGAGTAGATCAA+TGG - chr8.1:56550336-56550355 MS.gene068993:CDS 35.0%
GAGAAGAATCATCAAAGTCA+AGG - chr8.1:56549195-56549214 MS.gene068993:CDS 35.0%
GATTACTAGGGAGAATAAGT+TGG - chr8.1:56549635-56549654 MS.gene068993:CDS 35.0%
TGAATATGATCCAACCACAA+TGG - chr8.1:56549254-56549273 MS.gene068993:CDS 35.0%
TGCCAAGAAATAGTCCAATT+AGG - chr8.1:56550237-56550256 MS.gene068993:CDS 35.0%
TTCCTTTCCAACCAAGTAAA+AGG + chr8.1:56550481-56550500 None:intergenic 35.0%
! AAGTCATCAACTTCTGAAGA+AGG + chr8.1:56549126-56549145 None:intergenic 35.0%
! AGCATTTTCCTTTTGATCCT+TGG + chr8.1:56549734-56549753 None:intergenic 35.0%
! TAGCATGAATTTGCTTGTAG+AGG - chr8.1:56549899-56549918 MS.gene068993:CDS 35.0%
! TTGTCGATTGTAAGAAGAGA+AGG + chr8.1:56549981-56550000 None:intergenic 35.0%
! TTTTACTTGGTTGGAAAGGA+AGG - chr8.1:56550480-56550499 MS.gene068993:CDS 35.0%
!! AAACAGGCGAATTTGTTACT+TGG - chr8.1:56549459-56549478 MS.gene068993:CDS 35.0%
!! AGGTTTCGATCATCACTTTT+TGG - chr8.1:56549215-56549234 MS.gene068993:CDS 35.0%
!! TGTTGATAGCTTCATCAACA+TGG - chr8.1:56549146-56549165 MS.gene068993:CDS 35.0%
AATCCTGTTATCCTGAGCTT+TGG + chr8.1:56549843-56549862 None:intergenic 40.0%
ATATGATCCAACCACAATGG+AGG - chr8.1:56549257-56549276 MS.gene068993:CDS 40.0%
ATGGACCTTTCTTTCTTGGT+AGG - chr8.1:56550159-56550178 MS.gene068993:CDS 40.0%
CAAGAAAGAAAGGTCCATTG+TGG + chr8.1:56550157-56550176 None:intergenic 40.0%
CACAATGGACCTTTCTTTCT+TGG - chr8.1:56550155-56550174 MS.gene068993:CDS 40.0%
CATCATCAAGAACTGTCTCT+TGG + chr8.1:56549059-56549078 None:intergenic 40.0%
CTATTATGATGGCTTCTGCT+TGG - chr8.1:56550504-56550523 MS.gene068993:CDS 40.0%
CTTTGTAACGAATCTCCTGA+AGG + chr8.1:56549507-56549526 None:intergenic 40.0%
GAGCAAGATACAAACCACAA+TGG - chr8.1:56550140-56550159 MS.gene068993:CDS 40.0%
GATCAATGGAGAAGACAGTT+AGG - chr8.1:56550350-56550369 MS.gene068993:CDS 40.0%
GCAAATGAAGCTATATGCCA+AGG - chr8.1:56549714-56549733 MS.gene068993:CDS 40.0%
GCTATATGCCAAGGATCAAA+AGG - chr8.1:56549723-56549742 MS.gene068993:CDS 40.0%
GTAAGAAGAGAAGGAGTTGT+CGG + chr8.1:56549972-56549991 None:intergenic 40.0%
GTTGAAATGAACGGCAACTT+TGG - chr8.1:56549336-56549355 MS.gene068993:CDS 40.0%
TAACCTACACTTCAATGCTG+TGG + chr8.1:56549547-56549566 None:intergenic 40.0%
TATGAACCAAGGTCTTGACT+TGG - chr8.1:56549389-56549408 MS.gene068993:CDS 40.0%
TCATGTCTCTCCATTCTATC+AGG + chr8.1:56550323-56550342 None:intergenic 40.0%
TCTACAACTGTGAGTGCAAT+TGG + chr8.1:56550122-56550141 None:intergenic 40.0%
TTCAATGGCCTTCGCTAATA+AGG - chr8.1:56549790-56549809 MS.gene068993:CDS 40.0%
! ATTCACCTTCTCTCAAGTTG+AGG + chr8.1:56550004-56550023 None:intergenic 40.0%
! GAAAAGGGTTGCCTTTTACT+TGG - chr8.1:56550467-56550486 MS.gene068993:CDS 40.0%
! TGCCTTTTACTTGGTTGGAA+AGG - chr8.1:56550476-56550495 MS.gene068993:CDS 40.0%
AGAAGACAGTTAGGAAGAGC+AGG - chr8.1:56550359-56550378 MS.gene068993:CDS 45.0%
CAAGAACTGTCTCTTGGTCA+TGG + chr8.1:56549053-56549072 None:intergenic 45.0%
GAATGGAGCATGCTCTTCAA+TGG - chr8.1:56549775-56549794 MS.gene068993:CDS 45.0%
GACAACCTCAACTTGAGAGA+AGG - chr8.1:56549996-56550015 MS.gene068993:intron 45.0%
TAATAAGGAACCTCGCTTCG+AGG - chr8.1:56549805-56549824 MS.gene068993:CDS 45.0%
TATGGACCAAGTCAAGACCT+TGG + chr8.1:56549398-56549417 None:intergenic 45.0%
TCTTCATCCTCCATTGTGGT+TGG + chr8.1:56549267-56549286 None:intergenic 45.0%
TTGTGAGGGTCCTGATAGAA+TGG - chr8.1:56550310-56550329 MS.gene068993:CDS 45.0%
! AGGGTTGCCTTTTACTTGGT+TGG - chr8.1:56550471-56550490 MS.gene068993:CDS 45.0%
! TGCAACATTGTGGCTTGTGA+GGG - chr8.1:56550296-56550315 MS.gene068993:CDS 45.0%
! TTGCAACATTGTGGCTTGTG+AGG - chr8.1:56550295-56550314 MS.gene068993:CDS 45.0%
! TTGCCACAGCATTGAAGTGT+AGG - chr8.1:56549541-56549560 MS.gene068993:CDS 45.0%
! TTTGTTGTCTCTGCAGCCAA+CGG + chr8.1:56549442-56549461 None:intergenic 45.0%
!! GCTTGACTTGTACACTTGAG+TGG + chr8.1:56550398-56550417 None:intergenic 45.0%
!! GTAGAGGAAGCACTCTCATT+GGG - chr8.1:56549915-56549934 MS.gene068993:CDS 45.0%
!! TGTAGAGGAAGCACTCTCAT+TGG - chr8.1:56549914-56549933 MS.gene068993:CDS 45.0%
!! TTGGCTTTGTCAAGTCCTTC+AGG - chr8.1:56549489-56549508 MS.gene068993:CDS 45.0%
ACCTTGGTTCATACCGAGAC+TGG + chr8.1:56549382-56549401 None:intergenic 50.0%
CAATGCTGTGGCAAAGCAGT+AGG + chr8.1:56549535-56549554 None:intergenic 50.0%
CCACAATCACAAGCCAGTCT+CGG - chr8.1:56549366-56549385 MS.gene068993:CDS 50.0%
CCTCCAAAGCTCAGGATAAC+AGG - chr8.1:56549837-56549856 MS.gene068993:CDS 50.0%
GCGAGGTTCCTTATTAGCGA+AGG + chr8.1:56549801-56549820 None:intergenic 50.0%
TGGCTGCAGAGACAACAAAC+AGG - chr8.1:56549443-56549462 MS.gene068993:CDS 50.0%
! CCTGTTATCCTGAGCTTTGG+AGG + chr8.1:56549840-56549859 None:intergenic 50.0%
GATGGCTTCTGCTTGGCAAG+TGG - chr8.1:56550511-56550530 MS.gene068993:CDS 55.0%
GCCAGTCTCGGTATGAACCA+AGG - chr8.1:56549378-56549397 MS.gene068993:CDS 55.0%
!! CCGAGACTGGCTTGTGATTG+TGG + chr8.1:56549369-56549388 None:intergenic 55.0%
!! TGACTTGGTCCATACGCTGC+TGG - chr8.1:56549404-56549423 MS.gene068993:CDS 55.0%
AACCTCGCTTCGAGGCCTGA+GGG - chr8.1:56549813-56549832 MS.gene068993:CDS 60.0%
CAGCACAAGCCAGCAGCGTA+TGG + chr8.1:56549416-56549435 None:intergenic 60.0%
!! TACGCTGCTGGCTTGTGCTG+AGG - chr8.1:56549416-56549435 MS.gene068993:CDS 60.0%
ACCTCGCTTCGAGGCCTGAG+GGG - chr8.1:56549814-56549833 MS.gene068993:CDS 65.0%
CTGAGGGGCCTCCAAAGCTC+AGG - chr8.1:56549829-56549848 MS.gene068993:CDS 65.0%
GAACCTCGCTTCGAGGCCTG+AGG - chr8.1:56549812-56549831 MS.gene068993:CDS 65.0%
! CTGGCTTGTGCTGAGGCCGT+TGG - chr8.1:56549423-56549442 MS.gene068993:CDS 65.0%
!! CTGAGCTTTGGAGGCCCCTC+AGG + chr8.1:56549831-56549850 None:intergenic 65.0%
GCCCCTCAGGCCTCGAAGCG+AGG + chr8.1:56549818-56549837 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr8.1 gene 56549024 56550565 56549024 ID=MS.gene068993
chr8.1 mRNA 56549024 56550565 56549024 ID=MS.gene068993.t1;Parent=MS.gene068993
chr8.1 exon 56550027 56550565 56550027 ID=MS.gene068993.t1.exon1;Parent=MS.gene068993.t1
chr8.1 CDS 56550027 56550565 56550027 ID=cds.MS.gene068993.t1;Parent=MS.gene068993.t1
chr8.1 exon 56549024 56549945 56549024 ID=MS.gene068993.t1.exon2;Parent=MS.gene068993.t1
chr8.1 CDS 56549024 56549945 56549024 ID=cds.MS.gene068993.t1;Parent=MS.gene068993.t1
Gene Sequence

>MS.gene068993

ATGGAACAAGATCAAGAAGGAGATTGCCATGACCAAGAGACAGTTCTTGATGATGTTGAATTGTGTACTTTTGATACTTCAACCACTCCTTGTTTATTACCTTCTTCAGAAGTTGATGACTTTGTTGATAGCTTCATCAACATGGATCAATATGAAGATCAAGAGTCTCAAGAGAAGAATCATCAAAGTCAAGGTTTCGATCATCACTTTTTGGTGAATGATGATGTCTTTGAATATGATCCAACCACAATGGAGGATGAAGAATTTGAGATCTGTGAAAATTCAACAATCACAATGGAAGACGAAGAAGAAGTTGAAATGAACGGCAACTTTGGTGTTATTCCACAATCACAAGCCAGTCTCGGTATGAACCAAGGTCTTGACTTGGTCCATACGCTGCTGGCTTGTGCTGAGGCCGTTGGCTGCAGAGACAACAAACAGGCGAATTTGTTACTTGGTAAAATTTTGGCTTTGTCAAGTCCTTCAGGAGATTCGTTACAAAGAGTCTCCTACTGCTTTGCCACAGCATTGAAGTGTAGGTTATCACTTTATCCACATAATGTATTTTCAAATAGTACACTCACAACAAGTACTTCTAATGATGTTTCTTTGATTACTAGGGAGAATAAGTTGGAAGCTTTTCAACTTCTTTACCAAACAACACCTTATATTACTTTTGGTTTCATGGCTGCAAATGAAGCTATATGCCAAGGATCAAAAGGAAAATGCTCAATACACATAATTGATTTGGGAATGGAGCATGCTCTTCAATGGCCTTCGCTAATAAGGAACCTCGCTTCGAGGCCTGAGGGGCCTCCAAAGCTCAGGATAACAGGATTCAGTACGAATGAAGAAAACAACACAAAACTTCGCGCTAGCATGAATTTGCTTGTAGAGGAAGCACTCTCATTGGGAATAGTCCTTGAATTTCGCATAATATCAGAGCCGACAACTCCTTCTCTTCTTACAATCGACAACCTCAACTTGAGAGAAGGTGAATCTTTATTTGTCAATAGCATCTTGAAATTGCACAAATATGTGAAAGAAAGTAGAGGCTATCTAAAATCAATTCTTCAATCAATCAAGAAGCTAAGTCCAATTGCACTCACAGTTGTAGAGCAAGATACAAACCACAATGGACCTTTCTTTCTTGGTAGGTTTCTAGAATCACTTCACTATTACTCAGCAATATTTGATTCTCTTGAGGCAAGTATGCCAAGAAATAGTCCAATTAGGATGAAAATAGAGAGAGTACATTTTGCAGAAGAAATTTGCAACATTGTGGCTTGTGAGGGTCCTGATAGAATGGAGAGACATGAAAGAGTAGATCAATGGAGAAGACAGTTAGGAAGAGCAGGATTTCAAGTAATGCCACTCAAGTGTACAAGTCAAGCTAGAATGATGCTTTCTGTTTATGATTGTGATGGATATACATTGTCATGTGAAAAGGGTTGCCTTTTACTTGGTTGGAAAGGAAGGTCTATTATGATGGCTTCTGCTTGGCAAGTGGCAAGTTTGTCATATACTGATAGAGCCTCTTAA
Protein sequence

>MS.gene068993.t1

MEQDQEGDCHDQETVLDDVELCTFDTSTTPCLLPSSEVDDFVDSFINMDQYEDQESQEKNHQSQGFDHHFLVNDDVFEYDPTTMEDEEFEICENSTITMEDEEEVEMNGNFGVIPQSQASLGMNQGLDLVHTLLACAEAVGCRDNKQANLLLGKILALSSPSGDSLQRVSYCFATALKCRENKLEAFQLLYQTTPYITFGFMAANEAICQGSKGKCSIHIIDLGMEHALQWPSLIRNLASRPEGPPKLRITGFSTNEENNTKLRASMNLLVEEALSLGIVLEFRIISEPTTPSLLTIDNLNLREGESLFVNSILKLHKYVKESRGYLKSILQSIKKLSPIALTVVEQDTNHNGPFFLGRFLESLHYYSAIFDSLEASMPRNSPIRMKIERVHFAEEICNIVACEGPDRMERHERVDQWRRQLGRAGFQVMPLKCTSQARMMLSVYDCDGYTLSCEKGCLLLGWKGRSIMMASAWQVASLSYTDRAS