Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06915.t1 | XP_024636348.1 | 98.7 | 155 | 2 | 0 | 1 | 155 | 1 | 155 | 4.00E-83 | 317.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06915.t1 | Q9SZL8 | 33.3 | 153 | 97 | 3 | 20 | 168 | 76 | 227 | 5.8e-14 | 79.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06915.t1 | A0A396GJD5 | 76.2 | 164 | 39 | 0 | 1 | 164 | 1 | 164 | 6.5e-67 | 263.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene06915.t1 | TF | FAR1 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06915.t1 | MTR_6g051425 | 70.930 | 172 | 46 | 1 | 1 | 168 | 1 | 172 | 1.13e-87 | 260 |
| MS.gene06915.t1 | MTR_2g039370 | 82.432 | 74 | 13 | 0 | 49 | 122 | 98 | 171 | 5.65e-38 | 132 |
| MS.gene06915.t1 | MTR_3g465760 | 73.913 | 69 | 14 | 1 | 41 | 105 | 2 | 70 | 1.24e-29 | 103 |
| MS.gene06915.t1 | MTR_2g098880 | 30.579 | 121 | 74 | 3 | 7 | 121 | 51 | 167 | 1.64e-13 | 66.6 |
| MS.gene06915.t1 | MTR_2g098880 | 30.579 | 121 | 74 | 3 | 7 | 121 | 63 | 179 | 2.17e-13 | 66.2 |
| MS.gene06915.t1 | MTR_2g098920 | 29.126 | 103 | 72 | 1 | 18 | 120 | 81 | 182 | 3.77e-12 | 62.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06915.t1 | AT4G38180 | 34.228 | 149 | 93 | 3 | 20 | 164 | 76 | 223 | 1.71e-17 | 79.3 |
| MS.gene06915.t1 | AT3G59470 | 32.039 | 103 | 69 | 1 | 18 | 120 | 71 | 172 | 1.06e-12 | 64.3 |
| MS.gene06915.t1 | AT3G59470 | 32.039 | 103 | 69 | 1 | 18 | 120 | 71 | 172 | 1.06e-12 | 64.3 |
| MS.gene06915.t1 | AT3G59470 | 32.039 | 103 | 69 | 1 | 18 | 120 | 83 | 184 | 1.14e-12 | 64.3 |
| MS.gene06915.t1 | AT5G28530 | 28.477 | 151 | 101 | 2 | 18 | 163 | 56 | 204 | 7.69e-12 | 62.8 |
| MS.gene06915.t1 | AT5G28530 | 28.477 | 151 | 101 | 2 | 18 | 163 | 56 | 204 | 7.90e-12 | 62.8 |
| MS.gene06915.t1 | AT5G28530 | 28.477 | 151 | 101 | 2 | 18 | 163 | 56 | 204 | 3.38e-11 | 61.2 |
| MS.gene06915.t1 | AT5G28530 | 28.477 | 151 | 101 | 2 | 18 | 163 | 56 | 204 | 3.38e-11 | 61.2 |
| MS.gene06915.t1 | AT5G28530 | 28.477 | 151 | 101 | 2 | 18 | 163 | 56 | 204 | 3.59e-11 | 60.8 |
Find 34 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAAATATTTGGAATTATTT+AGG | 0.090273 | 3.2:-77056509 | MS.gene06915:CDS |
| GTATACGAGTTACAAATATT+TGG | 0.221426 | 3.2:-77056520 | MS.gene06915:CDS |
| CAAATATTTGGAATTATTTA+GGG | 0.245589 | 3.2:-77056508 | MS.gene06915:CDS |
| ATTATTCTTAAACCCTTCTT+TGG | 0.257903 | 3.2:+77056763 | None:intergenic |
| ATGTTAGCAAGGAAGGAGTT+TGG | 0.356508 | 3.2:-77056670 | MS.gene06915:CDS |
| GGGTTTAAGAATAATGAATA+TGG | 0.371754 | 3.2:-77056755 | MS.gene06915:CDS |
| AGGAGACCTCATAAAGTCTA+TGG | 0.381794 | 3.2:-77056552 | MS.gene06915:CDS |
| AACAGTAAAACTCTTTAAAA+CGG | 0.389800 | 3.2:+77056786 | None:intergenic |
| CAAGCACATGCCTTCAAAAC+AGG | 0.394361 | 3.2:-77056863 | MS.gene06915:CDS |
| CAGGATTTAGTGTAAGAAAA+GGG | 0.432600 | 3.2:-77056844 | MS.gene06915:CDS |
| TGTCAATCAGTTGAAATTGA+TGG | 0.438803 | 3.2:-77056947 | MS.gene06915:CDS |
| ACAGGATTTAGTGTAAGAAA+AGG | 0.440176 | 3.2:-77056845 | MS.gene06915:CDS |
| AGGTTTAATGTTAGCAAGGA+AGG | 0.442161 | 3.2:-77056677 | MS.gene06915:CDS |
| ATAAAGTCTATGGTGAATTC+TGG | 0.445432 | 3.2:-77056542 | MS.gene06915:CDS |
| GACATTTGACATATTTCTCA+TGG | 0.451386 | 3.2:+77056577 | None:intergenic |
| ACAGTAAAACTCTTTAAAAC+GGG | 0.452807 | 3.2:+77056787 | None:intergenic |
| AATTCACCATAGACTTTATG+AGG | 0.457499 | 3.2:+77056546 | None:intergenic |
| GTCGGTGGATATAACAATCT+TGG | 0.477476 | 3.2:-77056479 | None:intergenic |
| CTTATGAAAGAGCTGATTCA+AGG | 0.480001 | 3.2:-77056721 | MS.gene06915:CDS |
| CGCAACAAATGTCTTTGCTC+AGG | 0.482031 | 3.2:+77056602 | None:intergenic |
| TTGGAATTATTTAGGGGAAG+AGG | 0.485109 | 3.2:-77056501 | MS.gene06915:CDS |
| AAGGACAAATTGCAAAGCTA+TGG | 0.528165 | 3.2:-77056702 | MS.gene06915:CDS |
| AGAAATATGTCAAATGTCAA+AGG | 0.545496 | 3.2:-77056572 | MS.gene06915:CDS |
| ATTTAGTGTAAGAAAAGGGA+GGG | 0.556280 | 3.2:-77056840 | MS.gene06915:CDS |
| GATTTAGTGTAAGAAAAGGG+AGG | 0.567737 | 3.2:-77056841 | MS.gene06915:CDS |
| TAATGAATATGGAGGTGAAG+TGG | 0.572461 | 3.2:-77056744 | MS.gene06915:CDS |
| GGTGAGGTTTAATGTTAGCA+AGG | 0.579640 | 3.2:-77056681 | MS.gene06915:CDS |
| TATTTAGGGGAAGAGGTCGG+TGG | 0.586341 | 3.2:-77056494 | MS.gene06915:CDS |
| AATTATTTAGGGGAAGAGGT+CGG | 0.592715 | 3.2:-77056497 | MS.gene06915:CDS |
| TTTAAGAATAATGAATATGG+AGG | 0.604513 | 3.2:-77056752 | MS.gene06915:CDS |
| CTGAGCAAAGACATTTGTTG+CGG | 0.611498 | 3.2:-77056601 | MS.gene06915:CDS |
| CAAATTGCAAAGCTATGGTG+AGG | 0.629755 | 3.2:-77056697 | MS.gene06915:CDS |
| AAATATTTGGAATTATTTAG+GGG | 0.632966 | 3.2:-77056507 | MS.gene06915:CDS |
| AACAAATGTCTTTGCTCAGG+AGG | 0.641459 | 3.2:+77056605 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAATATTTGGAATTATTTAG+GGG | - | chr3.2:77056935-77056954 | MS.gene06915:CDS | 15.0% |
| !!! | ACAAATATTTGGAATTATTT+AGG | - | chr3.2:77056933-77056952 | MS.gene06915:CDS | 15.0% |
| !!! | CAAATATTTGGAATTATTTA+GGG | - | chr3.2:77056934-77056953 | MS.gene06915:CDS | 15.0% |
| !! | TTTAAGAATAATGAATATGG+AGG | - | chr3.2:77056690-77056709 | MS.gene06915:CDS | 20.0% |
| !!! | AACAGTAAAACTCTTTAAAA+CGG | + | chr3.2:77056659-77056678 | None:intergenic | 20.0% |
| ! | AGAAATATGTCAAATGTCAA+AGG | - | chr3.2:77056870-77056889 | MS.gene06915:CDS | 25.0% |
| ! | ATTATTCTTAAACCCTTCTT+TGG | + | chr3.2:77056682-77056701 | None:intergenic | 25.0% |
| ! | GGGTTTAAGAATAATGAATA+TGG | - | chr3.2:77056687-77056706 | MS.gene06915:CDS | 25.0% |
| ! | GTATACGAGTTACAAATATT+TGG | - | chr3.2:77056922-77056941 | MS.gene06915:CDS | 25.0% |
| !!! | ACAGTAAAACTCTTTAAAAC+GGG | + | chr3.2:77056658-77056677 | None:intergenic | 25.0% |
| AATTCACCATAGACTTTATG+AGG | + | chr3.2:77056899-77056918 | None:intergenic | 30.0% | |
| ACAGGATTTAGTGTAAGAAA+AGG | - | chr3.2:77056597-77056616 | MS.gene06915:CDS | 30.0% | |
| ATAAAGTCTATGGTGAATTC+TGG | - | chr3.2:77056900-77056919 | MS.gene06915:CDS | 30.0% | |
| ATTTAGTGTAAGAAAAGGGA+GGG | - | chr3.2:77056602-77056621 | MS.gene06915:CDS | 30.0% | |
| CAGGATTTAGTGTAAGAAAA+GGG | - | chr3.2:77056598-77056617 | MS.gene06915:CDS | 30.0% | |
| GACATTTGACATATTTCTCA+TGG | + | chr3.2:77056868-77056887 | None:intergenic | 30.0% | |
| TGTCAATCAGTTGAAATTGA+TGG | - | chr3.2:77056495-77056514 | MS.gene06915:CDS | 30.0% | |
| ! | AGTTTTACTGTTCCAAAGAA+GGG | - | chr3.2:77056667-77056686 | MS.gene06915:CDS | 30.0% |
| ! | GGATGCAAAGATATTTTTCT+TGG | - | chr3.2:77056516-77056535 | MS.gene06915:CDS | 30.0% |
| !! | TACACTAAATCCTGTTTTGA+AGG | + | chr3.2:77056592-77056611 | None:intergenic | 30.0% |
| AAGGACAAATTGCAAAGCTA+TGG | - | chr3.2:77056740-77056759 | MS.gene06915:CDS | 35.0% | |
| AATTATTTAGGGGAAGAGGT+CGG | - | chr3.2:77056945-77056964 | MS.gene06915:CDS | 35.0% | |
| AGGTTTAATGTTAGCAAGGA+AGG | - | chr3.2:77056765-77056784 | MS.gene06915:CDS | 35.0% | |
| GATTTAGTGTAAGAAAAGGG+AGG | - | chr3.2:77056601-77056620 | MS.gene06915:CDS | 35.0% | |
| TAATGAATATGGAGGTGAAG+TGG | - | chr3.2:77056698-77056717 | MS.gene06915:CDS | 35.0% | |
| TTGGAATTATTTAGGGGAAG+AGG | - | chr3.2:77056941-77056960 | MS.gene06915:CDS | 35.0% | |
| ! | CTTATGAAAGAGCTGATTCA+AGG | - | chr3.2:77056721-77056740 | MS.gene06915:CDS | 35.0% |
| ! | GAGTTTTACTGTTCCAAAGA+AGG | - | chr3.2:77056666-77056685 | MS.gene06915:CDS | 35.0% |
| AACAAATGTCTTTGCTCAGG+AGG | + | chr3.2:77056840-77056859 | None:intergenic | 40.0% | |
| AGGAGACCTCATAAAGTCTA+TGG | - | chr3.2:77056890-77056909 | MS.gene06915:CDS | 40.0% | |
| ATGTTAGCAAGGAAGGAGTT+TGG | - | chr3.2:77056772-77056791 | MS.gene06915:CDS | 40.0% | |
| CAAATTGCAAAGCTATGGTG+AGG | - | chr3.2:77056745-77056764 | MS.gene06915:CDS | 40.0% | |
| GGTGAGGTTTAATGTTAGCA+AGG | - | chr3.2:77056761-77056780 | MS.gene06915:CDS | 40.0% | |
| ! | CTGAGCAAAGACATTTGTTG+CGG | - | chr3.2:77056841-77056860 | MS.gene06915:CDS | 40.0% |
| CAAGCACATGCCTTCAAAAC+AGG | - | chr3.2:77056579-77056598 | MS.gene06915:CDS | 45.0% | |
| CGCAACAAATGTCTTTGCTC+AGG | + | chr3.2:77056843-77056862 | None:intergenic | 45.0% | |
| TATTTAGGGGAAGAGGTCGG+TGG | - | chr3.2:77056948-77056967 | MS.gene06915:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 77056480 | 77056984 | 77056480 | ID=MS.gene06915 |
| chr3.2 | mRNA | 77056480 | 77056984 | 77056480 | ID=MS.gene06915.t1;Parent=MS.gene06915 |
| chr3.2 | exon | 77056480 | 77056984 | 77056480 | ID=MS.gene06915.t1.exon1;Parent=MS.gene06915.t1 |
| chr3.2 | CDS | 77056480 | 77056984 | 77056480 | ID=cds.MS.gene06915.t1;Parent=MS.gene06915.t1 |
| Gene Sequence |
| Protein sequence |
