AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069224


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069224.t1 MTR_8g031360 98.086 209 4 0 5 213 89 297 2.23e-150 419
MS.gene069224.t1 MTR_7g076740 62.264 212 71 6 6 212 89 296 5.18e-78 236
MS.gene069224.t1 MTR_1g100653 38.914 221 93 8 6 212 88 280 1.21e-36 130
MS.gene069224.t1 MTR_7g011170 60.976 82 31 1 6 87 88 168 1.07e-25 101
MS.gene069224.t1 MTR_4g082290 59.770 87 23 3 6 85 88 169 1.04e-24 100
MS.gene069224.t1 MTR_4g082230 59.770 87 23 3 6 85 88 169 1.10e-24 100
MS.gene069224.t1 MTR_6g012690 52.727 110 38 4 6 111 88 187 1.56e-24 99.4
MS.gene069224.t1 MTR_5g042030 54.667 75 29 1 6 80 83 152 1.43e-19 84.7
MS.gene069224.t1 MTR_4g102380 78.571 42 9 0 6 47 88 129 1.39e-16 76.6
MS.gene069224.t1 MTR_3g077110 41.880 117 49 4 6 107 88 200 1.88e-16 77.0
MS.gene069224.t1 MTR_5g078140 73.333 45 12 0 6 50 88 132 4.47e-16 75.5
MS.gene069224.t1 MTR_4g091490 75.610 41 10 0 6 46 89 129 4.77e-16 75.9
MS.gene069224.t1 MTR_5g007370 73.171 41 11 0 6 46 88 128 6.28e-16 75.1
MS.gene069224.t1 MTR_3g074520 73.171 41 11 0 6 46 88 128 6.57e-16 75.5
MS.gene069224.t1 MTR_1g112760 72.093 43 12 0 5 47 88 130 8.42e-16 73.6
MS.gene069224.t1 MTR_2g011660 73.171 41 11 0 6 46 89 129 1.02e-15 74.7
MS.gene069224.t1 MTR_4g105130 65.217 46 16 0 6 51 88 133 1.68e-15 74.3
MS.gene069224.t1 MTR_4g478180 70.732 41 12 0 6 46 89 129 3.06e-15 73.6
MS.gene069224.t1 MTR_4g073420 39.000 100 56 1 6 105 88 182 4.15e-15 72.4
MS.gene069224.t1 MTR_2g064160 65.217 46 16 0 6 51 87 132 8.23e-15 72.0
MS.gene069224.t1 MTR_3g083540 69.048 42 13 0 6 47 117 158 1.02e-14 71.6
MS.gene069224.t1 MTR_1g085640 72.093 43 12 0 6 48 88 130 1.04e-14 72.4
MS.gene069224.t1 MTR_1g085040 72.093 43 12 0 6 48 88 130 1.20e-14 72.0
MS.gene069224.t1 MTR_2g089620 59.259 54 21 1 6 59 88 140 2.08e-14 70.9
MS.gene069224.t1 MTR_5g029840 65.854 41 14 0 6 46 88 128 3.07e-14 70.5
MS.gene069224.t1 MTR_7g110830 69.767 43 13 0 6 48 88 130 4.00e-14 70.5
MS.gene069224.t1 MTR_6g009430 62.791 43 16 0 6 48 88 130 4.13e-14 70.1
MS.gene069224.t1 MTR_3g461490 53.247 77 26 4 6 82 88 154 7.45e-14 69.3
MS.gene069224.t1 MTR_8g095390 61.364 44 17 0 6 49 88 131 7.63e-14 69.3
MS.gene069224.t1 MTR_3g065440 66.667 45 15 0 6 50 88 132 9.78e-14 68.9
MS.gene069224.t1 MTR_3g045430 67.442 43 14 0 6 48 89 131 1.15e-13 68.6
MS.gene069224.t1 MTR_5g079670 65.000 40 14 0 6 45 87 126 2.94e-13 66.2
MS.gene069224.t1 MTR_3g097450 48.387 62 28 1 6 63 88 149 5.66e-13 66.6
MS.gene069224.t1 MTR_4g485530 55.102 49 19 1 6 54 88 133 1.53e-12 63.9
MS.gene069224.t1 MTR_7g111290 69.767 43 13 0 6 48 46 88 2.00e-11 62.4
MS.gene069224.t1 MTR_5g007310 80.645 31 6 0 16 46 6 36 2.39e-11 61.2
MS.gene069224.t1 MTR_7g111290 69.767 43 13 0 6 48 88 130 3.50e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069224.t1 AT3G61250 50.704 213 78 7 6 213 88 278 1.48e-57 184
MS.gene069224.t1 AT5G15310 56.471 85 37 0 5 89 8 92 3.96e-28 106
MS.gene069224.t1 AT5G15310 57.143 84 36 0 6 89 88 171 5.11e-27 103
MS.gene069224.t1 AT3G01140 56.383 94 35 1 6 93 88 181 5.40e-27 105
MS.gene069224.t1 AT5G15310 57.143 84 36 0 6 89 88 171 5.67e-27 105
MS.gene069224.t1 AT5G15310 57.143 84 36 0 6 89 88 171 6.31e-27 105
MS.gene069224.t1 AT3G01140 56.383 94 35 1 6 93 131 224 1.36e-26 105
MS.gene069224.t1 AT1G22640 36.296 135 77 4 5 134 15 145 6.71e-17 75.9
MS.gene069224.t1 AT5G10280 39.815 108 40 2 6 92 88 191 1.43e-16 77.4
MS.gene069224.t1 AT5G61420 71.111 45 13 0 6 50 9 53 3.12e-16 75.9
MS.gene069224.t1 AT4G28110 70.732 41 12 0 6 46 4 44 4.78e-16 73.6
MS.gene069224.t1 AT5G65230 75.610 41 10 0 6 46 88 128 5.13e-16 75.5
MS.gene069224.t1 AT5G07700 66.000 50 17 0 6 55 88 137 6.53e-16 75.5
MS.gene069224.t1 AT5G07700 66.000 50 17 0 6 55 88 137 6.53e-16 75.5
MS.gene069224.t1 AT1G22640 37.607 117 66 3 6 119 31 143 6.83e-16 73.2
MS.gene069224.t1 AT4G22680 71.429 42 12 0 6 47 88 129 8.99e-16 74.3
MS.gene069224.t1 AT3G13540 69.048 42 13 0 6 47 99 140 1.53e-15 73.2
MS.gene069224.t1 AT5G61420 71.111 45 13 0 6 50 88 132 1.60e-15 74.3
MS.gene069224.t1 AT4G05100 70.732 41 12 0 6 46 89 129 1.85e-15 73.9
MS.gene069224.t1 AT3G02940 73.171 41 11 0 6 46 88 128 2.19e-15 73.6
MS.gene069224.t1 AT1G22640 37.607 117 66 4 6 119 88 200 2.54e-15 72.8
MS.gene069224.t1 AT4G21440 68.293 41 13 0 6 46 88 128 2.60e-15 73.9
MS.gene069224.t1 AT3G02940 73.171 41 11 0 6 46 88 128 2.94e-15 73.6
MS.gene069224.t1 AT3G02940 73.171 41 11 0 6 46 88 128 2.94e-15 73.6
MS.gene069224.t1 AT5G16770 73.171 41 11 0 6 46 88 128 3.18e-15 73.6
MS.gene069224.t1 AT5G16770 73.171 41 11 0 6 46 88 128 3.18e-15 73.6
MS.gene069224.t1 AT5G16770 73.171 41 11 0 6 46 88 128 4.26e-15 73.2
MS.gene069224.t1 AT5G16770 73.171 41 11 0 6 46 88 128 4.26e-15 73.2
MS.gene069224.t1 AT4G17785 70.732 41 12 0 6 46 89 129 4.29e-15 73.2
MS.gene069224.t1 AT5G16600 72.093 43 12 0 6 48 88 130 4.30e-15 73.2
MS.gene069224.t1 AT4G17785 70.732 41 12 0 6 46 89 129 4.86e-15 73.2
MS.gene069224.t1 AT1G34670 73.171 41 11 0 6 46 88 128 7.71e-15 72.4
MS.gene069224.t1 AT4G12350 69.048 42 13 0 6 47 88 129 8.93e-15 71.6
MS.gene069224.t1 AT5G54230 65.306 49 12 1 3 46 80 128 1.02e-14 72.0
MS.gene069224.t1 AT1G18570 68.889 45 14 0 6 50 89 133 1.23e-14 72.0
MS.gene069224.t1 AT5G62320 46.575 73 28 1 5 66 95 167 1.44e-14 70.5
MS.gene069224.t1 AT1G09540 64.583 48 17 0 6 53 88 135 2.12e-14 71.2
MS.gene069224.t1 AT4G28110 68.293 41 13 0 6 46 88 128 2.62e-14 70.5
MS.gene069224.t1 AT4G09460 64.444 45 16 0 6 50 88 132 3.12e-14 69.7
MS.gene069224.t1 AT4G34990 66.667 42 14 0 6 47 88 129 3.54e-14 70.1
MS.gene069224.t1 AT5G07690 69.767 43 13 0 6 48 88 130 5.20e-14 70.1
MS.gene069224.t1 AT1G57560 65.116 43 15 0 6 48 88 130 6.15e-14 69.7
MS.gene069224.t1 AT4G38620 62.222 45 17 0 6 50 88 132 6.22e-14 69.3
MS.gene069224.t1 AT2G16720 50.000 58 29 0 6 63 88 145 6.62e-14 68.9
MS.gene069224.t1 AT1G57560 65.116 43 15 0 6 48 88 130 8.33e-14 68.9
MS.gene069224.t1 AT1G74430 64.444 45 16 0 6 50 19 63 1.20e-13 67.4
MS.gene069224.t1 AT5G56110 67.442 43 14 0 6 48 88 130 1.29e-13 68.9
MS.gene069224.t1 AT1G35515 64.286 42 15 0 6 47 88 129 3.36e-13 66.2
MS.gene069224.t1 AT5G26660 63.636 44 16 0 6 49 57 100 3.96e-13 67.4
MS.gene069224.t1 AT4G01680 72.500 40 11 0 6 45 88 127 5.57e-13 66.2
MS.gene069224.t1 AT1G74430 64.444 45 16 0 6 50 88 132 5.67e-13 66.6
MS.gene069224.t1 AT5G26660 63.636 44 16 0 6 49 88 131 6.55e-13 67.0
MS.gene069224.t1 AT4G01680 72.500 40 11 0 6 45 88 127 7.95e-13 66.6
MS.gene069224.t1 AT4G01680 72.500 40 11 0 6 45 100 139 8.53e-13 66.6
MS.gene069224.t1 AT5G60890 65.217 46 16 0 5 50 87 132 1.28e-12 65.9
MS.gene069224.t1 AT5G14340 59.524 42 17 0 6 47 10 51 1.40e-11 61.2
MS.gene069224.t1 AT3G28470 49.383 81 29 2 6 74 88 168 2.22e-11 62.4
MS.gene069224.t1 AT3G12720 61.538 39 15 0 6 44 98 136 3.36e-11 61.6
MS.gene069224.t1 AT1G18710 64.286 42 15 0 6 47 88 129 7.47e-11 60.5
MS.gene069224.t1 AT1G74080 59.524 42 17 0 6 47 88 129 7.68e-11 60.8

Find 36 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACGAATTTGATTTGGAAAT+TGG 0.082125 8.2:+66986099 None:intergenic
CAATAACAACATTCCTTCTT+TGG 0.206897 8.2:-66985992 MS.gene069224:CDS
CTGACTATTTCCTTAAGATA+TGG 0.244127 8.2:-66985951 MS.gene069224:CDS
ACAATGAGATCAAGAATTTA+TGG 0.255794 8.2:-66986203 MS.gene069224:CDS
CACTGCACTGCAACTCTTGT+TGG 0.308906 8.2:-66985677 MS.gene069224:CDS
GGCGATAGACGAATTTGATT+TGG 0.316330 8.2:+66986092 None:intergenic
ATCTCATTGTCAGTTCTTCC+TGG 0.339461 8.2:+66986216 None:intergenic
CTTCAGAGTTCCATATCTTA+AGG 0.345177 8.2:+66985941 None:intergenic
ACGAATTTGATTTGGAAATT+GGG 0.374944 8.2:+66986100 None:intergenic
CACGCGCCACATGGCTCAAT+GGG 0.396446 8.2:-66986070 MS.gene069224:CDS
CCACGCGCCACATGGCTCAA+TGG 0.417264 8.2:-66986071 MS.gene069224:CDS
CTTCTTGAGACATTGAACTT+TGG 0.418411 8.2:+66985854 None:intergenic
AAGCTCATGTCATTGTTAGA+AGG 0.433035 8.2:+66985649 None:intergenic
AACAAATGAAACAATTTCAC+AGG 0.493206 8.2:-66986263 None:intergenic
AAGATATGGAACTCTGAAGT+TGG 0.497165 8.2:-66985937 MS.gene069224:CDS
GCTATTGGTTCATGAGTTTG+TGG 0.511715 8.2:+66986138 None:intergenic
GTCTATCGCCACGCGCCACA+TGG 0.519701 8.2:-66986079 MS.gene069224:CDS
AAAGAAACGTCTCATATGCA+TGG 0.523969 8.2:-66986169 MS.gene069224:CDS
TCAATTGCCTCTCAGCTACC+AGG 0.524112 8.2:-66986234 MS.gene069224:CDS
CACTTTCCCATTGAGCCATG+TGG 0.525677 8.2:+66986064 None:intergenic
CAAAGTTCAATGTCTCAAGA+AGG 0.548341 8.2:-66985853 MS.gene069224:CDS
AACTACAAGTCATTTGATGT+AGG 0.555746 8.2:-66985745 MS.gene069224:CDS
CTCGTCGTCTGATTTGCGAC+CGG 0.558757 8.2:+66985900 None:intergenic
TGTAATAACAACAACAAATG+TGG 0.560142 8.2:-66985823 MS.gene069224:CDS
TACTACAACAAGCAATGTGA+TGG 0.566980 8.2:-66985785 MS.gene069224:CDS
AAGAAACGTCTCATATGCAT+GGG 0.571800 8.2:-66986168 MS.gene069224:CDS
AAGTAATGTGATGAGGCTAT+TGG 0.582396 8.2:+66986123 None:intergenic
CAAGACTTGAAGCAGAAGCA+AGG 0.585545 8.2:-66986041 MS.gene069224:CDS
AAATGAAACAATTTCACAGG+TGG 0.587041 8.2:-66986260 MS.gene069224:CDS
AAAGTTCAATGTCTCAAGAA+GGG 0.602530 8.2:-66985852 MS.gene069224:CDS
CCATTGAGCCATGTGGCGCG+TGG 0.604740 8.2:+66986071 None:intergenic
AATGAAACAATTTCACAGGT+GGG 0.617428 8.2:-66986259 MS.gene069224:CDS
TACAACAAGCAATGTGATGG+AGG 0.620346 8.2:-66985782 MS.gene069224:CDS
AGTTCTTCCTGGTAGCTGAG+AGG 0.678055 8.2:+66986227 None:intergenic
CGGTCGCAAATCAGACGACG+AGG 0.685981 8.2:-66985899 MS.gene069224:CDS
AATTGGGAAGTAATGTGATG+AGG 0.686160 8.2:+66986116 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACAATGAGATCAAGAATTTA+TGG - chr8.2:66985694-66985713 MS.gene069224:CDS 25.0%
! TGTAATAACAACAACAAATG+TGG - chr8.2:66986074-66986093 MS.gene069224:CDS 25.0%
!!! ACGAATTTGATTTGGAAATT+GGG + chr8.2:66985800-66985819 None:intergenic 25.0%
AAAGTTCAATGTCTCAAGAA+GGG - chr8.2:66986045-66986064 MS.gene069224:CDS 30.0%
AACTACAAGTCATTTGATGT+AGG - chr8.2:66986152-66986171 MS.gene069224:CDS 30.0%
CAATAACAACATTCCTTCTT+TGG - chr8.2:66985905-66985924 MS.gene069224:CDS 30.0%
CTGACTATTTCCTTAAGATA+TGG - chr8.2:66985946-66985965 MS.gene069224:CDS 30.0%
!! GACGAATTTGATTTGGAAAT+TGG + chr8.2:66985801-66985820 None:intergenic 30.0%
AAAGAAACGTCTCATATGCA+TGG - chr8.2:66985728-66985747 MS.gene069224:CDS 35.0%
AAGAAACGTCTCATATGCAT+GGG - chr8.2:66985729-66985748 MS.gene069224:CDS 35.0%
AAGCTCATGTCATTGTTAGA+AGG + chr8.2:66986251-66986270 None:intergenic 35.0%
AAGTAATGTGATGAGGCTAT+TGG + chr8.2:66985777-66985796 None:intergenic 35.0%
AATTGGGAAGTAATGTGATG+AGG + chr8.2:66985784-66985803 None:intergenic 35.0%
CAAAGTTCAATGTCTCAAGA+AGG - chr8.2:66986044-66986063 MS.gene069224:CDS 35.0%
CTTCAGAGTTCCATATCTTA+AGG + chr8.2:66985959-66985978 None:intergenic 35.0%
CTTCTTGAGACATTGAACTT+TGG + chr8.2:66986046-66986065 None:intergenic 35.0%
TACTACAACAAGCAATGTGA+TGG - chr8.2:66986112-66986131 MS.gene069224:CDS 35.0%
!! AAGATATGGAACTCTGAAGT+TGG - chr8.2:66985960-66985979 MS.gene069224:CDS 35.0%
!! ATGTGATGGAGGATTTTGAA+TGG - chr8.2:66986126-66986145 MS.gene069224:CDS 35.0%
ATCTCATTGTCAGTTCTTCC+TGG + chr8.2:66985684-66985703 None:intergenic 40.0%
GGCGATAGACGAATTTGATT+TGG + chr8.2:66985808-66985827 None:intergenic 40.0%
TACAACAAGCAATGTGATGG+AGG - chr8.2:66986115-66986134 MS.gene069224:CDS 40.0%
!! GCTATTGGTTCATGAGTTTG+TGG + chr8.2:66985762-66985781 None:intergenic 40.0%
!! GTTTTGTTGCTTGCCAAAGA+AGG + chr8.2:66985921-66985940 None:intergenic 40.0%
CAAGACTTGAAGCAGAAGCA+AGG - chr8.2:66985856-66985875 MS.gene069224:CDS 45.0%
! GTTGGACAATCTTTTCGCAC+CGG - chr8.2:66985978-66985997 MS.gene069224:CDS 45.0%
AGTTCTTCCTGGTAGCTGAG+AGG + chr8.2:66985673-66985692 None:intergenic 50.0%
CACTGCACTGCAACTCTTGT+TGG - chr8.2:66986220-66986239 MS.gene069224:CDS 50.0%
CACTTTCCCATTGAGCCATG+TGG + chr8.2:66985836-66985855 None:intergenic 50.0%
TCAATTGCCTCTCAGCTACC+AGG - chr8.2:66985663-66985682 MS.gene069224:CDS 50.0%
! CTCGTCGTCTGATTTGCGAC+CGG + chr8.2:66986000-66986019 None:intergenic 55.0%
CACGCGCCACATGGCTCAAT+GGG - chr8.2:66985827-66985846 MS.gene069224:CDS 60.0%
CGGTCGCAAATCAGACGACG+AGG - chr8.2:66985998-66986017 MS.gene069224:CDS 60.0%
CCACGCGCCACATGGCTCAA+TGG - chr8.2:66985826-66985845 MS.gene069224:CDS 65.0%
CCATTGAGCCATGTGGCGCG+TGG + chr8.2:66985829-66985848 None:intergenic 65.0%
GTCTATCGCCACGCGCCACA+TGG - chr8.2:66985818-66985837 MS.gene069224:CDS 65.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 66985639 66986280 66985639 ID=MS.gene069224
chr8.2 mRNA 66985639 66986280 66985639 ID=MS.gene069224.t1;Parent=MS.gene069224
chr8.2 exon 66985639 66986280 66985639 ID=MS.gene069224.t1.exon1;Parent=MS.gene069224.t1
chr8.2 CDS 66985639 66986280 66985639 ID=cds.MS.gene069224.t1;Parent=MS.gene069224.t1
Gene Sequence

>MS.gene069224

ATGAAACAATTTCACAGGTGGGCTTCAATTGCCTCTCAGCTACCAGGAAGAACTGACAATGAGATCAAGAATTTATGGAACACACACTTAAAGAAACGTCTCATATGCATGGGAATTGATCCACAAACTCATGAACCAATAGCCTCATCACATTACTTCCCAATTTCCAAATCAAATTCGTCTATCGCCACGCGCCACATGGCTCAATGGGAAAGTGCAAGACTTGAAGCAGAAGCAAGGCTCTCAAAAGACACATCTTTAATCAACAATAACAACATTCCTTCTTTGGCAAGCAACAAAACTGATTCTGACTATTTCCTTAAGATATGGAACTCTGAAGTTGGACAATCTTTTCGCACCGGTCGCAAATCAGACGACGAGGACGATGACGATAAAACTAAGTGCCAAAGTTCAATGTCTCAAGAAGGGTCATCTTGTAATAACAACAACAAATGTGGATCAGTTTCTGTTACTACTACAACAAGCAATGTGATGGAGGATTTTGAATGGAGAAACTACAAGTCATTTGATGTAGGTTATGATGATTCATCAAGCTCTAATGATCTTGAAGACTCATCTGACACTGCACTGCAACTCTTGTTGGATTTTCCTTCTAACAATGACATGAGCTTCTTAGAATGA

Protein sequence

>MS.gene069224.t1

MKQFHRWASIASQLPGRTDNEIKNLWNTHLKKRLICMGIDPQTHEPIASSHYFPISKSNSSIATRHMAQWESARLEAEARLSKDTSLINNNNIPSLASNKTDSDYFLKIWNSEVGQSFRTGRKSDDEDDDDKTKCQSSMSQEGSSCNNNNKCGSVSVTTTTSNVMEDFEWRNYKSFDVGYDDSSSSNDLEDSSDTALQLLLDFPSNNDMSFLE