Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069300.t1 | XP_003598418.1 | 98.9 | 91 | 1 | 0 | 1 | 91 | 1 | 91 | 7.60E-44 | 186 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069300.t1 | Q58FS3 | 64.9 | 94 | 29 | 3 | 1 | 91 | 1 | 93 | 2.1e-26 | 119.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069300.t1 | G7IVT6 | 98.9 | 91 | 1 | 0 | 1 | 91 | 1 | 91 | 5.5e-44 | 186.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene069300.t1 | TF | MYB-related |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069300.t1 | MTR_3g013440 | 98.901 | 91 | 1 | 0 | 1 | 91 | 1 | 91 | 2.27e-64 | 189 |
| MS.gene069300.t1 | MTR_7g089210 | 71.053 | 76 | 21 | 1 | 12 | 87 | 14 | 88 | 1.43e-33 | 111 |
| MS.gene069300.t1 | MTR_1g022290 | 58.511 | 94 | 32 | 3 | 4 | 91 | 1 | 93 | 1.56e-33 | 111 |
| MS.gene069300.t1 | MTR_3g111880 | 58.242 | 91 | 33 | 2 | 4 | 90 | 1 | 90 | 8.14e-32 | 107 |
| MS.gene069300.t1 | MTR_6g004250 | 62.667 | 75 | 27 | 1 | 1 | 75 | 1 | 74 | 1.73e-30 | 103 |
| MS.gene069300.t1 | MTR_3g116720 | 64.198 | 81 | 22 | 2 | 1 | 75 | 1 | 80 | 6.61e-30 | 102 |
| MS.gene069300.t1 | MTR_8g077380 | 56.522 | 69 | 28 | 1 | 7 | 75 | 2 | 68 | 1.36e-23 | 85.5 |
| MS.gene069300.t1 | MTR_8g077390 | 56.522 | 69 | 28 | 1 | 7 | 75 | 2 | 68 | 1.36e-23 | 85.5 |
| MS.gene069300.t1 | MTR_8g077360 | 57.353 | 68 | 27 | 1 | 7 | 74 | 2 | 67 | 1.47e-23 | 85.5 |
| MS.gene069300.t1 | MTR_5g020540 | 59.375 | 64 | 24 | 1 | 8 | 71 | 2 | 63 | 4.89e-23 | 84.0 |
| MS.gene069300.t1 | MTR_8g077420 | 59.375 | 64 | 24 | 1 | 8 | 71 | 3 | 64 | 6.57e-23 | 84.0 |
| MS.gene069300.t1 | MTR_5g020170 | 48.649 | 74 | 37 | 1 | 1 | 74 | 10 | 82 | 2.00e-21 | 80.9 |
| MS.gene069300.t1 | MTR_5g081860 | 49.315 | 73 | 36 | 1 | 1 | 73 | 18 | 89 | 2.19e-17 | 74.3 |
| MS.gene069300.t1 | MTR_5g069710 | 46.429 | 84 | 33 | 2 | 9 | 81 | 28 | 110 | 8.40e-16 | 70.5 |
| MS.gene069300.t1 | MTR_7g067080 | 44.706 | 85 | 37 | 2 | 10 | 85 | 35 | 118 | 2.07e-15 | 69.3 |
| MS.gene069300.t1 | MTR_3g462790 | 50.704 | 71 | 34 | 1 | 3 | 73 | 36 | 105 | 4.16e-15 | 68.6 |
| MS.gene069300.t1 | MTR_1g033600 | 42.667 | 75 | 42 | 1 | 1 | 75 | 3 | 76 | 4.51e-15 | 65.9 |
| MS.gene069300.t1 | MTR_1g086180 | 52.632 | 57 | 27 | 0 | 10 | 66 | 11 | 67 | 7.22e-15 | 63.9 |
| MS.gene069300.t1 | MTR_7g110580 | 54.237 | 59 | 27 | 0 | 10 | 68 | 8 | 66 | 8.62e-15 | 63.9 |
| MS.gene069300.t1 | MTR_8g063600 | 46.575 | 73 | 38 | 1 | 1 | 73 | 1 | 72 | 1.27e-14 | 67.0 |
| MS.gene069300.t1 | MTR_8g063870 | 48.529 | 68 | 34 | 1 | 6 | 73 | 4 | 70 | 2.03e-14 | 66.2 |
| MS.gene069300.t1 | MTR_3g111920 | 41.667 | 72 | 41 | 1 | 9 | 80 | 2 | 72 | 2.09e-14 | 62.8 |
| MS.gene069300.t1 | MTR_4g111975 | 44.737 | 76 | 37 | 3 | 4 | 75 | 1 | 75 | 1.03e-12 | 62.0 |
| MS.gene069300.t1 | MTR_1g111830 | 40.909 | 66 | 38 | 1 | 10 | 75 | 24 | 88 | 3.87e-12 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene069300.t1 | AT4G39250 | 60.227 | 88 | 28 | 2 | 10 | 91 | 14 | 100 | 2.01e-34 | 114 |
| MS.gene069300.t1 | AT2G21650 | 61.798 | 89 | 26 | 2 | 10 | 91 | 14 | 101 | 2.33e-34 | 114 |
| MS.gene069300.t1 | AT1G75250 | 53.684 | 95 | 35 | 3 | 3 | 90 | 5 | 97 | 1.09e-27 | 96.7 |
| MS.gene069300.t1 | AT1G75250 | 60.811 | 74 | 28 | 1 | 3 | 76 | 5 | 77 | 2.23e-26 | 94.4 |
| MS.gene069300.t1 | AT1G19510 | 51.724 | 87 | 33 | 3 | 13 | 91 | 15 | 100 | 2.32e-22 | 83.6 |
| MS.gene069300.t1 | AT2G18328 | 63.492 | 63 | 22 | 1 | 13 | 75 | 14 | 75 | 4.68e-22 | 82.0 |
| MS.gene069300.t1 | AT4G36570 | 68.889 | 45 | 13 | 1 | 10 | 54 | 12 | 55 | 8.23e-17 | 68.2 |
| MS.gene069300.t1 | AT5G58900 | 52.000 | 75 | 32 | 2 | 2 | 73 | 23 | 96 | 1.23e-16 | 72.8 |
| MS.gene069300.t1 | AT5G05790 | 49.351 | 77 | 36 | 2 | 7 | 81 | 28 | 103 | 1.25e-16 | 72.4 |
| MS.gene069300.t1 | AT5G05790 | 49.351 | 77 | 36 | 2 | 7 | 81 | 28 | 103 | 1.25e-16 | 72.4 |
| MS.gene069300.t1 | AT2G38090 | 50.649 | 77 | 31 | 2 | 10 | 80 | 29 | 104 | 1.73e-16 | 72.4 |
| MS.gene069300.t1 | AT3G11280 | 48.438 | 64 | 32 | 1 | 10 | 73 | 33 | 95 | 8.17e-14 | 64.7 |
| MS.gene069300.t1 | AT3G11280 | 48.438 | 64 | 32 | 1 | 10 | 73 | 33 | 95 | 8.17e-14 | 64.7 |
| MS.gene069300.t1 | AT5G23650 | 47.222 | 72 | 37 | 1 | 4 | 75 | 7 | 77 | 1.51e-12 | 61.6 |
| MS.gene069300.t1 | AT5G08520 | 48.438 | 64 | 32 | 1 | 10 | 73 | 12 | 74 | 9.83e-12 | 59.3 |
| MS.gene069300.t1 | AT5G04760 | 39.706 | 68 | 39 | 1 | 6 | 73 | 2 | 67 | 2.62e-11 | 57.4 |
Find 25 sgRNAs with CRISPR-Local
Find 50 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCTCCAATGTTCTTGTAATT+TGG | 0.053146 | 3.3:-8405573 | None:intergenic |
| CTCCAATGTTCTTGTAATTT+GGG | 0.244052 | 3.3:-8405572 | None:intergenic |
| GACACTATGAACACCTTCTA+AGG | 0.262540 | 3.3:+8405514 | MS.gene069300:CDS |
| CATTTGGTTACTGGAGCTTC+AGG | 0.328788 | 3.3:-8406346 | None:intergenic |
| TATGCCTAACATCCCTTAGA+AGG | 0.404703 | 3.3:-8405527 | None:intergenic |
| CAATGTCAGCATCTGGTTCA+TGG | 0.413232 | 3.3:+8405370 | MS.gene069300:CDS |
| GATGTTAGGCATATTGAATC+AGG | 0.424945 | 3.3:+8405537 | MS.gene069300:CDS |
| AACACCTTCTAAGGGATGTT+AGG | 0.467379 | 3.3:+8405523 | MS.gene069300:CDS |
| TAGTGTCTCTTAACATCCTC+AGG | 0.475467 | 3.3:-8405498 | None:intergenic |
| CCAAATTACAAGAACATTGG+AGG | 0.482774 | 3.3:+8405573 | MS.gene069300:CDS |
| ATGGCATCAATGTCAGCATC+TGG | 0.486683 | 3.3:+8405363 | MS.gene069300:CDS |
| ACCAACAGCTTGAGCAACAT+TGG | 0.499603 | 3.3:-8405463 | None:intergenic |
| TTCCCAAATTACAAGAACAT+TGG | 0.502964 | 3.3:+8405570 | MS.gene069300:CDS |
| AGGACAATAAAGCATTTGAA+AGG | 0.506069 | 3.3:+8405400 | MS.gene069300:CDS |
| TCCAATGTTGCTCAAGCTGT+TGG | 0.520440 | 3.3:+8405462 | MS.gene069300:CDS |
| GGACAATAAAGCATTTGAAA+GGG | 0.535274 | 3.3:+8405401 | MS.gene069300:CDS |
| GCAACATTGGACCAACGGTC+AGG | 0.535547 | 3.3:-8405450 | None:intergenic |
| AATGTTGCTCAAGCTGTTGG+TGG | 0.546053 | 3.3:+8405465 | MS.gene069300:CDS |
| GTTGCTCAAGCTGTTGGTGG+TGG | 0.554528 | 3.3:+8405468 | MS.gene069300:CDS |
| ATCTGGTTCATGGAGTGCTA+AGG | 0.583163 | 3.3:+8405380 | MS.gene069300:CDS |
| ATAAAGACACACCTGACCGT+TGG | 0.596639 | 3.3:+8405439 | MS.gene069300:CDS |
| ACACTATGAACACCTTCTAA+GGG | 0.610400 | 3.3:+8405515 | MS.gene069300:CDS |
| CTTGAGCAACATTGGACCAA+CGG | 0.640722 | 3.3:-8405455 | None:intergenic |
| GAACATTGGAGGCTATGATG+AGG | 0.660409 | 3.3:+8405584 | MS.gene069300:CDS |
| TGGTGGTGGAAAGACTCCTG+AGG | 0.678384 | 3.3:+8405482 | MS.gene069300:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTATATGTACTAATTTTT+GGG | + | chr3.3:8406032-8406051 | MS.gene069300:intron | 10.0% |
| !!! | TATTTATATGTACTAATTTT+TGG | + | chr3.3:8406031-8406050 | MS.gene069300:intron | 10.0% |
| !!! | TTTTCTTATAAACATATATT+AGG | + | chr3.3:8405635-8405654 | MS.gene069300:intron | 10.0% |
| !! | ATAGTAAGAATGAAAAATAA+TGG | + | chr3.3:8405930-8405949 | MS.gene069300:intron | 15.0% |
| !! | TCTTATAAACATATATTAGG+AGG | + | chr3.3:8405638-8405657 | MS.gene069300:intron | 20.0% |
| !!! | AAGTTATAAGGTGTTTTAAA+AGG | + | chr3.3:8406181-8406200 | MS.gene069300:intron | 20.0% |
| !!! | ACACAAGTTGAATAAAAAAT+TGG | - | chr3.3:8406127-8406146 | None:intergenic | 20.0% |
| !!! | ACATGTCAAATTTTGAATAA+AGG | - | chr3.3:8406094-8406113 | None:intergenic | 20.0% |
| !!! | AGAATTTGATAGCATAAATT+CGG | - | chr3.3:8405969-8405988 | None:intergenic | 20.0% |
| !! | GAATTTGATAGCATAAATTC+GGG | - | chr3.3:8405968-8405987 | None:intergenic | 25.0% |
| ACCTTATAACTTAAGACGAA+TGG | - | chr3.3:8406173-8406192 | None:intergenic | 30.0% | |
| CTCCAATGTTCTTGTAATTT+GGG | - | chr3.3:8405575-8405594 | None:intergenic | 30.0% | |
| TCAAGCATAATTCATAAGAG+TGG | + | chr3.3:8406003-8406022 | MS.gene069300:intron | 30.0% | |
| TCCATTCGTCTTAAGTTATA+AGG | + | chr3.3:8406169-8406188 | MS.gene069300:intron | 30.0% | |
| TTCCCAAATTACAAGAACAT+TGG | + | chr3.3:8405570-8405589 | MS.gene069300:CDS | 30.0% | |
| ! | AGGACAATAAAGCATTTGAA+AGG | + | chr3.3:8405400-8405419 | MS.gene069300:CDS | 30.0% |
| ! | GGACAATAAAGCATTTGAAA+GGG | + | chr3.3:8405401-8405420 | MS.gene069300:CDS | 30.0% |
| ! | GGAGTAAAAAAGTGTAAGTT+GGG | - | chr3.3:8406152-8406171 | None:intergenic | 30.0% |
| ! | TGGAGTAAAAAAGTGTAAGT+TGG | - | chr3.3:8406153-8406172 | None:intergenic | 30.0% |
| !! | ACGCTGAATAAGTTTTATGA+AGG | - | chr3.3:8405684-8405703 | None:intergenic | 30.0% |
| !!! | TACTAATTTTTGGGTCAAGT+GGG | + | chr3.3:8406041-8406060 | MS.gene069300:intron | 30.0% |
| ACACTATGAACACCTTCTAA+GGG | + | chr3.3:8405515-8405534 | MS.gene069300:CDS | 35.0% | |
| AGCATAAATTCGGGTATGTT+CGG | - | chr3.3:8405959-8405978 | None:intergenic | 35.0% | |
| CCAAATTACAAGAACATTGG+AGG | + | chr3.3:8405573-8405592 | MS.gene069300:CDS | 35.0% | |
| CCTCCAATGTTCTTGTAATT+TGG | - | chr3.3:8405576-8405595 | None:intergenic | 35.0% | |
| CTTATATACACATGTAGTCC+AGG | - | chr3.3:8405728-8405747 | None:intergenic | 35.0% | |
| GATGTTAGGCATATTGAATC+AGG | + | chr3.3:8405537-8405556 | MS.gene069300:CDS | 35.0% | |
| !!! | ACTAATTTTTGGGTCAAGTG+GGG | + | chr3.3:8406042-8406061 | MS.gene069300:intron | 35.0% |
| !!! | GTACTAATTTTTGGGTCAAG+TGG | + | chr3.3:8406040-8406059 | MS.gene069300:intron | 35.0% |
| GACACTATGAACACCTTCTA+AGG | + | chr3.3:8405514-8405533 | MS.gene069300:CDS | 40.0% | |
| GAGGCTATGATGAGGAAAAA+AGG | + | chr3.3:8405592-8405611 | MS.gene069300:CDS | 40.0% | |
| TAGTGTCTCTTAACATCCTC+AGG | - | chr3.3:8405501-8405520 | None:intergenic | 40.0% | |
| TATGCCTAACATCCCTTAGA+AGG | - | chr3.3:8405530-8405549 | None:intergenic | 40.0% | |
| ! | AACACCTTCTAAGGGATGTT+AGG | + | chr3.3:8405523-8405542 | MS.gene069300:CDS | 40.0% |
| !! | AACTCATATCGCTGATTTCC+TGG | + | chr3.3:8405707-8405726 | MS.gene069300:intron | 40.0% |
| AATGTTGCTCAAGCTGTTGG+TGG | + | chr3.3:8405465-8405484 | MS.gene069300:CDS | 45.0% | |
| ACCAACAGCTTGAGCAACAT+TGG | - | chr3.3:8405466-8405485 | None:intergenic | 45.0% | |
| ATAAAGACACACCTGACCGT+TGG | + | chr3.3:8405439-8405458 | MS.gene069300:CDS | 45.0% | |
| ATGGCATCAATGTCAGCATC+TGG | + | chr3.3:8405363-8405382 | MS.gene069300:CDS | 45.0% | |
| CTTGAGCAACATTGGACCAA+CGG | - | chr3.3:8405458-8405477 | None:intergenic | 45.0% | |
| GAACATTGGAGGCTATGATG+AGG | + | chr3.3:8405584-8405603 | MS.gene069300:CDS | 45.0% | |
| TCCAATGTTGCTCAAGCTGT+TGG | + | chr3.3:8405462-8405481 | MS.gene069300:CDS | 45.0% | |
| ! | ATCTGGTTCATGGAGTGCTA+AGG | + | chr3.3:8405380-8405399 | MS.gene069300:CDS | 45.0% |
| ! | CAATGTCAGCATCTGGTTCA+TGG | + | chr3.3:8405370-8405389 | MS.gene069300:CDS | 45.0% |
| AACCTGCAGGTGAAAGCAAG+AGG | - | chr3.3:8406320-8406339 | None:intergenic | 50.0% | |
| ! | CTTCAGGTTTCTCAACCTGC+AGG | - | chr3.3:8406333-8406352 | None:intergenic | 50.0% |
| GCAACATTGGACCAACGGTC+AGG | - | chr3.3:8405453-8405472 | None:intergenic | 55.0% | |
| TGCCTCTTGCTTTCACCTGC+AGG | + | chr3.3:8406315-8406334 | MS.gene069300:intron | 55.0% | |
| ! | GTTGCTCAAGCTGTTGGTGG+TGG | + | chr3.3:8405468-8405487 | MS.gene069300:CDS | 55.0% |
| !! | TGGTGGTGGAAAGACTCCTG+AGG | + | chr3.3:8405482-8405501 | MS.gene069300:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 8405363 | 8406361 | 8405363 | ID=MS.gene069300 |
| chr3.3 | mRNA | 8405363 | 8406361 | 8405363 | ID=MS.gene069300.t1;Parent=MS.gene069300 |
| chr3.3 | exon | 8405363 | 8405613 | 8405363 | ID=MS.gene069300.t1.exon1;Parent=MS.gene069300.t1 |
| chr3.3 | CDS | 8405363 | 8405613 | 8405363 | ID=cds.MS.gene069300.t1;Parent=MS.gene069300.t1 |
| chr3.3 | exon | 8406337 | 8406361 | 8406337 | ID=MS.gene069300.t1.exon2;Parent=MS.gene069300.t1 |
| chr3.3 | CDS | 8406337 | 8406361 | 8406337 | ID=cds.MS.gene069300.t1;Parent=MS.gene069300.t1 |
| Gene Sequence |
| Protein sequence |
