AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene069538

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069538.t1 MTR_8g017210 33.908 174 99 7 75 247 69 227 4.73e-22 95.9
MS.gene069538.t1 MTR_2g093960 31.000 200 122 7 76 275 42 225 2.06e-21 93.2
MS.gene069538.t1 MTR_1g093095 33.714 175 105 7 75 241 89 260 3.09e-21 91.3
MS.gene069538.t1 MTR_4g059870 33.523 176 101 7 77 251 47 207 9.50e-21 92.0
MS.gene069538.t1 MTR_2g099990 32.402 179 105 7 75 252 53 216 1.11e-20 91.7
MS.gene069538.t1 MTR_2g090745 33.333 159 94 6 69 227 10 156 2.90e-19 87.4
MS.gene069538.t1 MTR_1g112270 32.895 152 90 6 76 227 54 193 3.03e-19 87.8
MS.gene069538.t1 MTR_1g112270 31.325 166 100 7 64 227 51 204 6.76e-19 86.7
MS.gene069538.t1 MTR_1g094115 34.211 152 88 6 76 227 57 196 1.20e-18 85.9
MS.gene069538.t1 MTR_1g016010 35.065 154 89 6 75 227 48 191 2.69e-18 84.7
MS.gene069538.t1 MTR_2g099990 32.716 162 94 6 91 252 1 147 3.65e-18 84.0
MS.gene069538.t1 MTR_1g094115 35.115 131 74 5 97 227 60 179 6.86e-16 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene069538.t1 AT1G34370 51.872 187 84 3 77 258 15 200 2.02e-65 206
MS.gene069538.t1 AT1G34370 51.872 187 84 3 77 258 225 410 3.59e-64 209
MS.gene069538.t1 AT1G34370 51.872 187 84 3 77 258 225 410 3.59e-64 209
MS.gene069538.t1 AT5G22890 49.261 203 95 3 52 247 170 371 1.91e-61 199
MS.gene069538.t1 AT5G66730 36.364 154 86 6 74 227 40 181 2.61e-22 96.7
MS.gene069538.t1 AT5G60470 34.416 154 90 5 75 227 51 194 4.70e-22 95.5
MS.gene069538.t1 AT5G44160 35.065 154 88 6 75 227 44 186 5.22e-22 95.5
MS.gene069538.t1 AT5G60470 34.416 154 90 5 75 227 47 190 5.43e-22 95.5
MS.gene069538.t1 AT3G45260 32.022 178 106 6 75 251 46 209 8.39e-22 94.7
MS.gene069538.t1 AT3G45260 32.022 178 106 6 75 251 46 209 8.39e-22 94.7
MS.gene069538.t1 AT1G08290 39.007 141 80 5 98 236 182 318 1.14e-21 93.2
MS.gene069538.t1 AT1G13290 37.415 147 87 4 98 242 103 246 1.80e-21 92.0
MS.gene069538.t1 AT1G03840 35.065 154 88 6 75 227 48 190 2.53e-21 93.6
MS.gene069538.t1 AT5G03150 35.526 152 87 5 76 227 63 203 4.12e-21 93.2
MS.gene069538.t1 AT5G44160 34.459 148 86 5 80 227 11 147 4.79e-21 92.4
MS.gene069538.t1 AT1G51220 39.189 148 83 6 98 243 180 322 5.05e-21 91.7
MS.gene069538.t1 AT5G60470 33.557 149 89 4 79 227 47 185 5.09e-21 92.4
MS.gene069538.t1 AT3G50700 36.145 166 92 7 62 227 32 183 7.79e-21 92.0
MS.gene069538.t1 AT1G03840 34.641 153 88 6 75 227 48 188 9.38e-21 92.0
MS.gene069538.t1 AT1G55110 32.948 173 101 6 75 246 70 228 1.17e-20 91.7
MS.gene069538.t1 AT1G55110 32.948 173 101 6 75 246 70 228 1.17e-20 91.7
MS.gene069538.t1 AT1G55110 32.948 173 101 6 75 246 70 228 1.17e-20 91.7
MS.gene069538.t1 AT3G20880 37.107 159 93 6 98 254 258 411 2.71e-20 90.1
MS.gene069538.t1 AT3G13810 35.065 154 89 5 75 227 77 220 4.28e-20 90.1
MS.gene069538.t1 AT3G13810 35.065 154 89 5 75 227 77 220 4.28e-20 90.1
MS.gene069538.t1 AT3G13810 35.065 154 89 5 75 227 72 215 4.35e-20 90.1
MS.gene069538.t1 AT3G13810 35.065 154 89 5 75 227 58 201 4.97e-20 90.1
MS.gene069538.t1 AT4G02670 35.714 154 88 6 75 227 60 203 1.86e-19 87.8
MS.gene069538.t1 AT4G02670 35.714 154 88 6 75 227 62 205 1.95e-19 87.8
MS.gene069538.t1 AT2G02080 30.645 186 109 8 76 257 64 233 3.99e-19 87.4
MS.gene069538.t1 AT2G02080 30.645 186 109 8 76 257 64 233 3.99e-19 87.4
MS.gene069538.t1 AT2G02080 30.645 186 109 8 76 257 64 233 3.99e-19 87.4
MS.gene069538.t1 AT2G02080 30.645 186 109 8 76 257 64 233 3.99e-19 87.4
MS.gene069538.t1 AT3G13810 34.459 148 87 4 80 227 84 221 4.36e-19 87.4
MS.gene069538.t1 AT3G13810 34.459 148 87 4 80 227 70 207 4.44e-19 87.0
MS.gene069538.t1 AT1G14580 32.895 152 90 6 76 227 63 202 7.67e-19 86.3
MS.gene069538.t1 AT1G14580 32.895 152 90 6 76 227 63 202 7.67e-19 86.3
MS.gene069538.t1 AT1G34790 35.329 167 97 8 89 249 137 298 8.43e-19 84.7
MS.gene069538.t1 AT1G14580 32.895 152 90 6 76 227 77 216 9.12e-19 86.3
MS.gene069538.t1 AT2G02070 31.757 148 90 5 80 227 65 201 3.66e-18 84.7
MS.gene069538.t1 AT2G02070 31.757 148 90 5 80 227 65 201 3.66e-18 84.7
MS.gene069538.t1 AT3G57670 35.862 145 86 6 98 240 219 358 9.02e-18 82.8
MS.gene069538.t1 AT1G25250 34.839 155 90 6 74 227 18 162 3.71e-17 80.9
MS.gene069538.t1 AT2G01940 35.897 156 82 8 76 227 54 195 7.61e-17 80.5
MS.gene069538.t1 AT1G25250 35.294 153 88 6 76 227 43 185 7.65e-17 80.1
MS.gene069538.t1 AT2G02080 29.825 171 101 7 91 257 1 156 2.26e-16 79.0
MS.gene069538.t1 AT2G02080 29.825 171 101 7 91 257 1 156 2.26e-16 79.0
MS.gene069538.t1 AT1G68130 35.294 153 87 6 77 227 50 192 3.62e-16 78.2
MS.gene069538.t1 AT2G01940 34.043 141 81 6 76 215 54 183 6.74e-14 71.6

Find 70 sgRNAs with CRISPR-Local

Find 82 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGAGGAATTGTTATTGTTTC+TGG 0.086343 2.3:-42115129 None:intergenic
TTGATGATTTGGATAAATTT+TGG 0.104736 2.3:+42115808 MS.gene069538:CDS
GGAAGGTTTCTTTGATGATT+TGG 0.263746 2.3:+42115797 MS.gene069538:CDS
TTCCTCCAGCCGAGTGTTAC+TGG 0.282881 2.3:+42115145 MS.gene069538:CDS
CATGTCATCGTCAATGTAAT+TGG 0.286361 2.3:-42115184 None:intergenic
TCCACTTCTGCGAGATTTGC+GGG 0.292814 2.3:+42115268 MS.gene069538:CDS
TGCGTCGAGTTCCACAATTT+TGG 0.303992 2.3:-42115223 None:intergenic
ATGAAGGTTGCAAACGTAAC+AGG 0.321705 2.3:+42115424 MS.gene069538:CDS
GACAATGACTTCTCTTGAAA+TGG 0.326614 2.3:-42115494 None:intergenic
AGTGAGTCTAAGTGTTGTTT+GGG 0.344560 2.3:-42115039 None:intergenic
TCTAGGAAGGAGAAATTGTT+TGG 0.374626 2.3:+42115639 MS.gene069538:CDS
TGACGATGACATGGCAAATC+CGG 0.381522 2.3:+42115194 MS.gene069538:CDS
GACGAATTGTGGAGGAGATT+TGG 0.390719 2.3:-42115068 None:intergenic
GAGAAGTGTGTTTGTTTGAA+TGG 0.397224 2.3:-42115016 None:intergenic
TGAAAACGGTGTTCTGTTTG+AGG 0.428042 2.3:+42115469 MS.gene069538:CDS
GAGTGAGTCTAAGTGTTGTT+TGG 0.432423 2.3:-42115040 None:intergenic
GACGGCGGCGCCGGCAGAAG+AGG 0.442327 2.3:+42115722 MS.gene069538:CDS
TTACGTTTGCAACCTTCATA+AGG 0.442930 2.3:-42115420 None:intergenic
TGTGTTTGTTTGAATGGATT+GGG 0.447482 2.3:-42115010 None:intergenic
GGACACGTGGAGCGGTTTGA+AGG 0.462859 2.3:+42115660 MS.gene069538:CDS
TTCTGCGAGATTTGCGGGAA+AGG 0.464202 2.3:+42115273 MS.gene069538:CDS
TTCCACTTCTGCGAGATTTG+CGG 0.470603 2.3:+42115267 MS.gene069538:CDS
GTGTGTTTGTTTGAATGGAT+TGG 0.474691 2.3:-42115011 None:intergenic
AATTGTGGAGGAGATTTGGT+TGG 0.477694 2.3:-42115064 None:intergenic
TGATTGCATAGATACGTTAC+CGG 0.493467 2.3:+42115776 MS.gene069538:CDS
GGAAAAGCAAGCGCGTTTAA+CGG 0.496000 2.3:+42115380 MS.gene069538:CDS
AAAATCAATGACGGCGGCGC+CGG 0.497008 2.3:+42115713 MS.gene069538:CDS
CCCACACCTTCCTCTTCTGC+CGG 0.501400 2.3:-42115732 None:intergenic
GAAGTTCAATCCGTTGAAAA+CGG 0.526797 2.3:+42115455 MS.gene069538:CDS
AAATCATCAAAGAAACCTTC+CGG 0.527715 2.3:-42115795 None:intergenic
CCGGCGGTTGTTGAAGAGAA+AGG 0.528093 2.3:+42115690 MS.gene069538:CDS
CCTGTCCAGTAACACTCGGC+TGG 0.529625 2.3:-42115150 None:intergenic
TCCCGCAAATCTCGCAGAAG+TGG 0.537900 2.3:-42115269 None:intergenic
GAGTCACGTGAAACAGTGTA+GGG 0.546406 2.3:+42115578 MS.gene069538:CDS
TCCGGACCATTCCAAAATTG+TGG 0.553263 2.3:+42115212 MS.gene069538:CDS
GATGATGACTTGACGAATTG+TGG 0.554147 2.3:-42115079 None:intergenic
TGTTATTGTTTCTGGACAAG+TGG 0.555781 2.3:-42115121 None:intergenic
CGTATGCACATGCGCGCGCA+CGG 0.560123 2.3:+42115318 MS.gene069538:CDS
CCAGCCGAGTGTTACTGGAC+AGG 0.560795 2.3:+42115150 MS.gene069538:CDS
GGAGAAATTGTTTGGACACG+TGG 0.562238 2.3:+42115647 MS.gene069538:CDS
CCGGCAGAAGAGGAAGGTGT+GGG 0.568129 2.3:+42115732 MS.gene069538:CDS
CAATTACATTGACGATGACA+TGG 0.570490 2.3:+42115185 MS.gene069538:CDS
CGAAAACTCACACGTGCGAT+CGG 0.572334 2.3:+42115517 MS.gene069538:CDS
CTCACACGTGCGATCGGTGT+CGG 0.577837 2.3:+42115523 MS.gene069538:CDS
TGCATAGATACGTTACCGGA+AGG 0.579492 2.3:+42115780 MS.gene069538:CDS
GCCGGCAGAAGAGGAAGGTG+TGG 0.587117 2.3:+42115731 MS.gene069538:CDS
CCTTTCTCTTCAACAACCGC+CGG 0.591632 2.3:-42115690 None:intergenic
AGAGTCACGTGAAACAGTGT+AGG 0.598901 2.3:+42115577 MS.gene069538:CDS
GCGGCGCCGGCAGAAGAGGA+AGG 0.609998 2.3:+42115726 MS.gene069538:CDS
GCGGTTTGAAGGACACTCGC+CGG 0.611639 2.3:+42115671 MS.gene069538:CDS
TCCACGTGGAAGTGTTCGTG+TGG 0.623338 2.3:+42115606 MS.gene069538:CDS
GTCCAGTAACACTCGGCTGG+AGG 0.629005 2.3:-42115147 None:intergenic
GCAGAAGAGGAAGGTGTGGG+TGG 0.632135 2.3:+42115735 MS.gene069538:CDS
ATGAAGCGTTACGTAGCGTG+AGG 0.636493 2.3:+42115358 MS.gene069538:CDS
AATTGTGGAACTCGACGCAG+TGG 0.638379 2.3:+42115227 MS.gene069538:CDS
TTTCTCCTTCCTAGAAAACG+TGG 0.641293 2.3:-42115631 None:intergenic
GCCGAGTGTTACTGGACAGG+TGG 0.642781 2.3:+42115153 MS.gene069538:CDS
AATTGTTTGGACACGTGGAG+CGG 0.644804 2.3:+42115652 MS.gene069538:CDS
GTTTGAAGGACACTCGCCGG+CGG 0.649562 2.3:+42115674 MS.gene069538:CDS
ACCACCTGTCCAGTAACACT+CGG 0.660156 2.3:-42115154 None:intergenic
ACACGTGCGATCGGTGTCGG+AGG 0.660805 2.3:+42115526 MS.gene069538:CDS
AGAGAAAGGAAAATCAATGA+CGG 0.662591 2.3:+42115704 MS.gene069538:CDS
TGAAGCGTTACGTAGCGTGA+GGG 0.667496 2.3:+42115359 MS.gene069538:CDS
GAAAGGAAAATCAATGACGG+CGG 0.669060 2.3:+42115707 MS.gene069538:CDS
GCGCGCGCATGTGCATACGT+AGG 0.669149 2.3:-42115314 None:intergenic
GTGAGTCTAAGTGTTGTTTG+GGG 0.679107 2.3:-42115038 None:intergenic
AGTGTAGGGGAGAGTCCACG+TGG 0.705085 2.3:+42115592 MS.gene069538:CDS
GATGACTTGACGAATTGTGG+AGG 0.731309 2.3:-42115076 None:intergenic
AGTCACGTGAAACAGTGTAG+GGG 0.737402 2.3:+42115579 MS.gene069538:CDS
TCCACACGAACACTTCCACG+TGG 0.747159 2.3:-42115607 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATGATTTGGATAAATTTT+GGG + chr2.3:42115809-42115828 MS.gene069538:CDS 20.0%
!!! TTGATGATTTGGATAAATTT+TGG + chr2.3:42115808-42115827 MS.gene069538:CDS 20.0%
AAATCATCAAAGAAACCTTC+CGG - chr2.3:42115798-42115817 None:intergenic 30.0%
AGAGAAAGGAAAATCAATGA+CGG + chr2.3:42115704-42115723 MS.gene069538:CDS 30.0%
! TGTGTTTGTTTGAATGGATT+GGG - chr2.3:42115013-42115032 None:intergenic 30.0%
CAATTACATTGACGATGACA+TGG + chr2.3:42115185-42115204 MS.gene069538:CDS 35.0%
CATGTCATCGTCAATGTAAT+TGG - chr2.3:42115187-42115206 None:intergenic 35.0%
GAAGTTCAATCCGTTGAAAA+CGG + chr2.3:42115455-42115474 MS.gene069538:CDS 35.0%
GACAATGACTTCTCTTGAAA+TGG - chr2.3:42115497-42115516 None:intergenic 35.0%
GTGTGTTTGTTTGAATGGAT+TGG - chr2.3:42115014-42115033 None:intergenic 35.0%
TCTAGGAAGGAGAAATTGTT+TGG + chr2.3:42115639-42115658 MS.gene069538:CDS 35.0%
TGATTGCATAGATACGTTAC+CGG + chr2.3:42115776-42115795 MS.gene069538:CDS 35.0%
TTACGTTTGCAACCTTCATA+AGG - chr2.3:42115423-42115442 None:intergenic 35.0%
! AAACAGAACACCGTTTTCAA+CGG - chr2.3:42115468-42115487 None:intergenic 35.0%
! CGATTTTCTTGTCCTTATGA+AGG + chr2.3:42115408-42115427 MS.gene069538:CDS 35.0%
! GAGAAGTGTGTTTGTTTGAA+TGG - chr2.3:42115019-42115038 None:intergenic 35.0%
! GGAAGGTTTCTTTGATGATT+TGG + chr2.3:42115797-42115816 MS.gene069538:CDS 35.0%
! GGAGGAATTGTTATTGTTTC+TGG - chr2.3:42115132-42115151 None:intergenic 35.0%
!! AGTGAGTCTAAGTGTTGTTT+GGG - chr2.3:42115042-42115061 None:intergenic 35.0%
!! TGTTATTGTTTCTGGACAAG+TGG - chr2.3:42115124-42115143 None:intergenic 35.0%
ATGAAGGTTGCAAACGTAAC+AGG + chr2.3:42115424-42115443 MS.gene069538:CDS 40.0%
GAAAGGAAAATCAATGACGG+CGG + chr2.3:42115707-42115726 MS.gene069538:CDS 40.0%
GATGATGACTTGACGAATTG+TGG - chr2.3:42115082-42115101 None:intergenic 40.0%
TGAAAACGGTGTTCTGTTTG+AGG + chr2.3:42115469-42115488 MS.gene069538:CDS 40.0%
TTTCTCCTTCCTAGAAAACG+TGG - chr2.3:42115634-42115653 None:intergenic 40.0%
! AATTGTGGAGGAGATTTGGT+TGG - chr2.3:42115067-42115086 None:intergenic 40.0%
! GAGTGAGTCTAAGTGTTGTT+TGG - chr2.3:42115043-42115062 None:intergenic 40.0%
! TGTGGGTGGATATTTTACGA+AGG + chr2.3:42115749-42115768 MS.gene069538:CDS 40.0%
!! CGAGTTCCACAATTTTGGAA+TGG - chr2.3:42115221-42115240 None:intergenic 40.0%
!! GTGAGTCTAAGTGTTGTTTG+GGG - chr2.3:42115041-42115060 None:intergenic 40.0%
!!! TCCACAATTTTGGAATGGTC+CGG - chr2.3:42115216-42115235 None:intergenic 40.0%
AATTGTTTGGACACGTGGAG+CGG + chr2.3:42115652-42115671 MS.gene069538:CDS 45.0%
AGAGTCACGTGAAACAGTGT+AGG + chr2.3:42115577-42115596 MS.gene069538:CDS 45.0%
AGTCACGTGAAACAGTGTAG+GGG + chr2.3:42115579-42115598 MS.gene069538:CDS 45.0%
GACGAATTGTGGAGGAGATT+TGG - chr2.3:42115071-42115090 None:intergenic 45.0%
GAGTCACGTGAAACAGTGTA+GGG + chr2.3:42115578-42115597 MS.gene069538:CDS 45.0%
GATGACTTGACGAATTGTGG+AGG - chr2.3:42115079-42115098 None:intergenic 45.0%
GGAAAAGCAAGCGCGTTTAA+CGG + chr2.3:42115380-42115399 MS.gene069538:CDS 45.0%
GGAGAAATTGTTTGGACACG+TGG + chr2.3:42115647-42115666 MS.gene069538:CDS 45.0%
TCCGGACCATTCCAAAATTG+TGG + chr2.3:42115212-42115231 MS.gene069538:CDS 45.0%
TGACGATGACATGGCAAATC+CGG + chr2.3:42115194-42115213 MS.gene069538:CDS 45.0%
TGCATAGATACGTTACCGGA+AGG + chr2.3:42115780-42115799 MS.gene069538:CDS 45.0%
TTCCACTTCTGCGAGATTTG+CGG + chr2.3:42115267-42115286 MS.gene069538:CDS 45.0%
! TGCGTCGAGTTCCACAATTT+TGG - chr2.3:42115226-42115245 None:intergenic 45.0%
!! TGGAACCACGTTTTCTAGGA+AGG + chr2.3:42115626-42115645 MS.gene069538:CDS 45.0%
AATTGTGGAACTCGACGCAG+TGG + chr2.3:42115227-42115246 MS.gene069538:CDS 50.0%
ACCACCTGTCCAGTAACACT+CGG - chr2.3:42115157-42115176 None:intergenic 50.0%
CCTTTCTCTTCAACAACCGC+CGG - chr2.3:42115693-42115712 None:intergenic 50.0%
CGAAAACTCACACGTGCGAT+CGG + chr2.3:42115517-42115536 MS.gene069538:CDS 50.0%
TCCACTTCTGCGAGATTTGC+GGG + chr2.3:42115268-42115287 MS.gene069538:CDS 50.0%
TTCTGCGAGATTTGCGGGAA+AGG + chr2.3:42115273-42115292 MS.gene069538:CDS 50.0%
! ATGAAGCGTTACGTAGCGTG+AGG + chr2.3:42115358-42115377 MS.gene069538:CDS 50.0%
! CGATCGCACGTGTGAGTTTT+CGG - chr2.3:42115519-42115538 None:intergenic 50.0%
! CGTGTGGAACCACGTTTTCT+AGG + chr2.3:42115622-42115641 MS.gene069538:CDS 50.0%
! GAAGAGTTTTGCGGTGGTGT+CGG + chr2.3:42115548-42115567 MS.gene069538:CDS 50.0%
! GTGTCGGAGGAAGAGTTTTG+CGG + chr2.3:42115539-42115558 MS.gene069538:CDS 50.0%
! TGAAGCGTTACGTAGCGTGA+GGG + chr2.3:42115359-42115378 MS.gene069538:CDS 50.0%
AAAATCAATGACGGCGGCGC+CGG + chr2.3:42115713-42115732 MS.gene069538:CDS 55.0%
CCGGCGGTTGTTGAAGAGAA+AGG + chr2.3:42115690-42115709 MS.gene069538:CDS 55.0%
TCCACACGAACACTTCCACG+TGG - chr2.3:42115610-42115629 None:intergenic 55.0%
TCCCGCAAATCTCGCAGAAG+TGG - chr2.3:42115272-42115291 None:intergenic 55.0%
TTCCTCCAGCCGAGTGTTAC+TGG + chr2.3:42115145-42115164 MS.gene069538:CDS 55.0%
! TCGGAGGAAGAGTTTTGCGG+TGG + chr2.3:42115542-42115561 MS.gene069538:CDS 55.0%
!! TCCACGTGGAAGTGTTCGTG+TGG + chr2.3:42115606-42115625 MS.gene069538:CDS 55.0%
AGTGTAGGGGAGAGTCCACG+TGG + chr2.3:42115592-42115611 MS.gene069538:CDS 60.0%
CCCACACCTTCCTCTTCTGC+CGG - chr2.3:42115735-42115754 None:intergenic 60.0%
CCTGTCCAGTAACACTCGGC+TGG - chr2.3:42115153-42115172 None:intergenic 60.0%
CTCACACGTGCGATCGGTGT+CGG + chr2.3:42115523-42115542 MS.gene069538:CDS 60.0%
GCGGTTTGAAGGACACTCGC+CGG + chr2.3:42115671-42115690 MS.gene069538:CDS 60.0%
GGACACGTGGAGCGGTTTGA+AGG + chr2.3:42115660-42115679 MS.gene069538:CDS 60.0%
GTCCAGTAACACTCGGCTGG+AGG - chr2.3:42115150-42115169 None:intergenic 60.0%
GTTTGAAGGACACTCGCCGG+CGG + chr2.3:42115674-42115693 MS.gene069538:CDS 60.0%
! CCAGCCGAGTGTTACTGGAC+AGG + chr2.3:42115150-42115169 MS.gene069538:CDS 60.0%
! CCGGCAGAAGAGGAAGGTGT+GGG + chr2.3:42115732-42115751 MS.gene069538:CDS 60.0%
! GCAGAAGAGGAAGGTGTGGG+TGG + chr2.3:42115735-42115754 MS.gene069538:CDS 60.0%
! GCCGAGTGTTACTGGACAGG+TGG + chr2.3:42115153-42115172 MS.gene069538:CDS 60.0%
CGTATGCACATGCGCGCGCA+CGG + chr2.3:42115318-42115337 MS.gene069538:CDS 65.0%
GCCGGCAGAAGAGGAAGGTG+TGG + chr2.3:42115731-42115750 MS.gene069538:CDS 65.0%
GCGCGCGCATGTGCATACGT+AGG - chr2.3:42115317-42115336 None:intergenic 65.0%
!! ACACGTGCGATCGGTGTCGG+AGG + chr2.3:42115526-42115545 MS.gene069538:CDS 65.0%
!! GCGGCGCCGGCAGAAGAGGA+AGG + chr2.3:42115726-42115745 MS.gene069538:CDS 75.0%
!!! GACGGCGGCGCCGGCAGAAG+AGG + chr2.3:42115722-42115741 MS.gene069538:CDS 80.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 42114985 42115836 42114985 ID=MS.gene069538
chr2.3 mRNA 42114985 42115836 42114985 ID=MS.gene069538.t1;Parent=MS.gene069538
chr2.3 exon 42114985 42115836 42114985 ID=MS.gene069538.t1.exon1;Parent=MS.gene069538.t1
chr2.3 CDS 42114985 42115836 42114985 ID=cds.MS.gene069538.t1;Parent=MS.gene069538.t1
Gene Sequence

>MS.gene069538

ATGGATTCTATTCAAAGCTTCATATCCCAATCCATTCAAACAAACACACTTCTCCCCAAACAACACTTAGACTCACTCTCCAACCAAATCTCCTCCACAATTCGTCAAGTCATCATCAACGCAACTGCTCTTCTCGCCACTTGTCCAGAAACAATAACAATTCCTCCAGCCGAGTGTTACTGGACAGGTGGTGAGAAAACCAATTACATTGACGATGACATGGCAAATCCGGACCATTCCAAAATTGTGGAACTCGACGCAGTGGAACTGCTTGCGAAGCACTTCCACTTCTGCGAGATTTGCGGGAAAGGTTTCACGCGTGATGCAAACCTACGTATGCACATGCGCGCGCACGGAGAACAGTTCAAGACACATGAAGCGTTACGTAGCGTGAGGGAAAAGCAAGCGCGTTTAACGGCGACGCGATTTTCTTGTCCTTATGAAGGTTGCAAACGTAACAGGTTACACAAGAAGTTCAATCCGTTGAAAACGGTGTTCTGTTTGAGGAACCATTTCAAGAGAAGTCATTGTCCGAAAACTCACACGTGCGATCGGTGTCGGAGGAAGAGTTTTGCGGTGGTGTCGGATTTGAAGAGTCACGTGAAACAGTGTAGGGGAGAGTCCACGTGGAAGTGTTCGTGTGGAACCACGTTTTCTAGGAAGGAGAAATTGTTTGGACACGTGGAGCGGTTTGAAGGACACTCGCCGGCGGTTGTTGAAGAGAAAGGAAAATCAATGACGGCGGCGCCGGCAGAAGAGGAAGGTGTGGGTGGATATTTTACGAAGGAAAATGATTGCATAGATACGTTACCGGAAGGTTTCTTTGATGATTTGGATAAATTTTGGGTTTGA
Protein sequence

>MS.gene069538.t1

MDSIQSFISQSIQTNTLLPKQHLDSLSNQISSTIRQVIINATALLATCPETITIPPAECYWTGGEKTNYIDDDMANPDHSKIVELDAVELLAKHFHFCEICGKGFTRDANLRMHMRAHGEQFKTHEALRSVREKQARLTATRFSCPYEGCKRNRLHKKFNPLKTVFCLRNHFKRSHCPKTHTCDRCRRKSFAVVSDLKSHVKQCRGESTWKCSCGTTFSRKEKLFGHVERFEGHSPAVVEEKGKSMTAAPAEEEGVGGYFTKENDCIDTLPEGFFDDLDKFWV