Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06983.t1 | KEH19403.1 | 68.6 | 137 | 35 | 2 | 11 | 147 | 18 | 146 | 1.10E-42 | 183 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06983.t1 | Q9FNG6 | 50.7 | 150 | 24 | 1 | 18 | 117 | 129 | 278 | 6.7e-33 | 141.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06983.t1 | A0A072U5J1 | 68.6 | 137 | 35 | 2 | 11 | 147 | 18 | 146 | 7.8e-43 | 183.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene06983.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06983.t1 | MTR_7g108868 | 68.613 | 137 | 35 | 2 | 11 | 147 | 18 | 146 | 1.13e-61 | 188 |
| MS.gene06983.t1 | MTR_8g058593 | 68.613 | 137 | 35 | 2 | 11 | 147 | 18 | 146 | 1.13e-61 | 188 |
| MS.gene06983.t1 | MTR_8g058607 | 78.378 | 111 | 22 | 1 | 11 | 121 | 31 | 139 | 5.77e-60 | 182 |
| MS.gene06983.t1 | MTR_7g109250 | 50.785 | 191 | 26 | 3 | 1 | 124 | 51 | 240 | 4.10e-54 | 173 |
| MS.gene06983.t1 | MTR_7g109250 | 50.000 | 190 | 29 | 2 | 1 | 124 | 111 | 300 | 4.17e-53 | 172 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene06983.t1 | AT5G06420 | 49.375 | 160 | 31 | 1 | 18 | 127 | 129 | 288 | 5.02e-45 | 151 |
| MS.gene06983.t1 | AT5G06420 | 49.375 | 160 | 31 | 1 | 18 | 127 | 129 | 288 | 5.02e-45 | 151 |
| MS.gene06983.t1 | AT1G01350 | 48.750 | 160 | 32 | 1 | 18 | 127 | 94 | 253 | 6.27e-44 | 147 |
Find 28 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAGGGAAAAGGACTTTC+TGG | 0.207237 | 7.3:+6332841 | MS.gene06983:CDS |
| GCGCACACTCTTTGCTTTCT+CGG | 0.276894 | 7.3:-6332780 | None:intergenic |
| GACCGAGGCGACTACAATTC+CGG | 0.339363 | 7.3:+6332728 | MS.gene06983:CDS |
| TGAAGACTCAAAATGAAAGT+TGG | 0.349810 | 7.3:-6332489 | None:intergenic |
| AAAATGAAAGTTGGGCTTCT+CGG | 0.382812 | 7.3:-6332480 | None:intergenic |
| TTGAAGTCTTTGCATATGTC+AGG | 0.387439 | 7.3:-6332659 | None:intergenic |
| GAAGACTCAAAATGAAAGTT+GGG | 0.391037 | 7.3:-6332488 | None:intergenic |
| TGACATATGCAAAGACTTCA+AGG | 0.411330 | 7.3:+6332661 | MS.gene06983:CDS |
| ACCGAGGCGACTACAATTCC+GGG | 0.427387 | 7.3:+6332729 | MS.gene06983:CDS |
| TAGCAAAGCAACAGCAACTT+TGG | 0.436558 | 7.3:+6332535 | MS.gene06983:CDS |
| TGCAAAGACTTCAAGGAGAC+CGG | 0.477457 | 7.3:+6332668 | MS.gene06983:CDS |
| GGTGGCAGTTGGACAAAGAA+TGG | 0.496757 | 7.3:+6332750 | MS.gene06983:CDS |
| AAAGAGTGTGCGCGCGCTGA+TGG | 0.507062 | 7.3:+6332790 | MS.gene06983:CDS |
| TTGTCTGTTTGTTTGTCTGA+AGG | 0.512605 | 7.3:+6332814 | MS.gene06983:CDS |
| TTCAAGGAGACCGGGTATTG+TGG | 0.545327 | 7.3:+6332677 | MS.gene06983:CDS |
| CTACAATTCCGGGTGGCAGT+TGG | 0.548426 | 7.3:+6332739 | MS.gene06983:CDS |
| ACCCGGAATTGTAGTCGCCT+CGG | 0.550971 | 7.3:-6332730 | None:intergenic |
| TTCTTTGTCCAACTGCCACC+CGG | 0.562902 | 7.3:-6332747 | None:intergenic |
| TGCAAGTTTCTTCACGACCG+AGG | 0.564969 | 7.3:+6332713 | MS.gene06983:CDS |
| GAGACCGGGTATTGTGGCTA+CGG | 0.578795 | 7.3:+6332683 | MS.gene06983:CDS |
| ATCGCCGTAGCCACAATACC+CGG | 0.586901 | 7.3:-6332687 | None:intergenic |
| GAAACTCTAATATGAGTCGA+AGG | 0.595574 | 7.3:-6332617 | None:intergenic |
| ACATGTAAATTACAAACACC+CGG | 0.625741 | 7.3:-6332882 | None:intergenic |
| GTGTGAGTGTGACCCACGGA+TGG | 0.632397 | 7.3:-6332582 | None:intergenic |
| GCAAAGACTTCAAGGAGACC+GGG | 0.633254 | 7.3:+6332669 | MS.gene06983:CDS |
| GACTGTGTGAGTGTGACCCA+CGG | 0.673754 | 7.3:-6332586 | None:intergenic |
| ATATGAGTCGAAGGTCTGAG+AGG | 0.683131 | 7.3:-6332608 | None:intergenic |
| GAGGCGACTACAATTCCGGG+TGG | 0.758896 | 7.3:+6332732 | MS.gene06983:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAAGACTCAAAATGAAAGTT+GGG | - | chr7.3:6332491-6332510 | None:intergenic | 30.0% | |
| TGAAGACTCAAAATGAAAGT+TGG | - | chr7.3:6332492-6332511 | None:intergenic | 30.0% | |
| TGAGAAAAAGAACATGAAGA+AGG | + | chr7.3:6332439-6332458 | MS.gene06983:CDS | 30.0% | |
| TTGTCTGAAGGAAAAAAGAA+GGG | + | chr7.3:6332826-6332845 | MS.gene06983:CDS | 30.0% | |
| TTTGTCTGAAGGAAAAAAGA+AGG | + | chr7.3:6332825-6332844 | MS.gene06983:CDS | 30.0% | |
| AAAATGAAAGTTGGGCTTCT+CGG | - | chr7.3:6332483-6332502 | None:intergenic | 35.0% | |
| GAAACTCTAATATGAGTCGA+AGG | - | chr7.3:6332620-6332639 | None:intergenic | 35.0% | |
| GAAGGAAAAAAGAAGGGAAA+AGG | + | chr7.3:6332832-6332851 | MS.gene06983:CDS | 35.0% | |
| TGACATATGCAAAGACTTCA+AGG | + | chr7.3:6332661-6332680 | MS.gene06983:CDS | 35.0% | |
| TTGAAGTCTTTGCATATGTC+AGG | - | chr7.3:6332662-6332681 | None:intergenic | 35.0% | |
| TTGTCTGTTTGTTTGTCTGA+AGG | + | chr7.3:6332814-6332833 | MS.gene06983:CDS | 35.0% | |
| !! | ATTTTGCTGACTACAATTCC+GGG | + | chr7.3:6332864-6332883 | MS.gene06983:CDS | 35.0% |
| !! | GATTTTGCTGACTACAATTC+CGG | + | chr7.3:6332863-6332882 | MS.gene06983:CDS | 35.0% |
| !!! | ATTTTTCAAGTTCCATCCGT+GGG | + | chr7.3:6332570-6332589 | MS.gene06983:CDS | 35.0% |
| AAGAAGGGAAAAGGACTTTC+TGG | + | chr7.3:6332841-6332860 | MS.gene06983:CDS | 40.0% | |
| TAGCAAAGCAACAGCAACTT+TGG | + | chr7.3:6332535-6332554 | MS.gene06983:CDS | 40.0% | |
| !!! | CATTTTTCAAGTTCCATCCG+TGG | + | chr7.3:6332569-6332588 | MS.gene06983:CDS | 40.0% |
| ATATGAGTCGAAGGTCTGAG+AGG | - | chr7.3:6332611-6332630 | None:intergenic | 45.0% | |
| TGCAAAGACTTCAAGGAGAC+CGG | + | chr7.3:6332668-6332687 | MS.gene06983:CDS | 45.0% | |
| GCAAAGACTTCAAGGAGACC+GGG | + | chr7.3:6332669-6332688 | MS.gene06983:CDS | 50.0% | |
| GCGCACACTCTTTGCTTTCT+CGG | - | chr7.3:6332783-6332802 | None:intergenic | 50.0% | |
| GGTGGCAGTTGGACAAAGAA+TGG | + | chr7.3:6332750-6332769 | MS.gene06983:CDS | 50.0% | |
| TGCAAGTTTCTTCACGACCG+AGG | + | chr7.3:6332713-6332732 | MS.gene06983:CDS | 50.0% | |
| TTCTTTGTCCAACTGCCACC+CGG | - | chr7.3:6332750-6332769 | None:intergenic | 50.0% | |
| ! | TTCAAGGAGACCGGGTATTG+TGG | + | chr7.3:6332677-6332696 | MS.gene06983:CDS | 50.0% |
| ACCCGGAATTGTAGTCGCCT+CGG | - | chr7.3:6332733-6332752 | None:intergenic | 55.0% | |
| ACCGAGGCGACTACAATTCC+GGG | + | chr7.3:6332729-6332748 | MS.gene06983:CDS | 55.0% | |
| ATCGCCGTAGCCACAATACC+CGG | - | chr7.3:6332690-6332709 | None:intergenic | 55.0% | |
| CTACAATTCCGGGTGGCAGT+TGG | + | chr7.3:6332739-6332758 | MS.gene06983:CDS | 55.0% | |
| GACCGAGGCGACTACAATTC+CGG | + | chr7.3:6332728-6332747 | MS.gene06983:CDS | 55.0% | |
| GACTGTGTGAGTGTGACCCA+CGG | - | chr7.3:6332589-6332608 | None:intergenic | 55.0% | |
| ! | GAGACCGGGTATTGTGGCTA+CGG | + | chr7.3:6332683-6332702 | MS.gene06983:CDS | 55.0% |
| GAGGCGACTACAATTCCGGG+TGG | + | chr7.3:6332732-6332751 | MS.gene06983:CDS | 60.0% | |
| GTGTGAGTGTGACCCACGGA+TGG | - | chr7.3:6332585-6332604 | None:intergenic | 60.0% | |
| ! | AAAGAGTGTGCGCGCGCTGA+TGG | + | chr7.3:6332790-6332809 | MS.gene06983:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.3 | gene | 6332431 | 6332895 | 6332431 | ID=MS.gene06983 |
| chr7.3 | mRNA | 6332431 | 6332895 | 6332431 | ID=MS.gene06983.t1;Parent=MS.gene06983 |
| chr7.3 | exon | 6332431 | 6332895 | 6332431 | ID=MS.gene06983.t1.exon1;Parent=MS.gene06983.t1 |
| chr7.3 | CDS | 6332431 | 6332895 | 6332431 | ID=cds.MS.gene06983.t1;Parent=MS.gene06983.t1 |
| Gene Sequence |
| Protein sequence |
