AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07006


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07006.t1 MTR_8g058557 70.787 178 46 3 7 182 1 174 3.83e-81 244
MS.gene07006.t1 MTR_7g108870 70.787 178 46 3 7 182 1 174 7.15e-81 244
MS.gene07006.t1 MTR_7g108870 70.787 178 46 3 7 182 1 174 1.95e-79 244
MS.gene07006.t1 MTR_8g058590 70.787 178 46 3 7 182 1 174 1.95e-79 244
MS.gene07006.t1 MTR_8g058607 82.301 113 18 1 139 251 31 141 1.44e-63 195
MS.gene07006.t1 MTR_7g108868 81.982 111 18 1 139 249 18 126 1.77e-62 194
MS.gene07006.t1 MTR_8g058593 81.982 111 18 1 139 249 18 126 1.77e-62 194
MS.gene07006.t1 MTR_7g109250 51.852 189 23 3 128 249 50 237 2.51e-54 178
MS.gene07006.t1 MTR_7g109250 51.852 189 23 3 128 249 110 297 2.72e-53 177
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07006.t1 AT2G47790 53.416 161 67 4 20 176 25 181 4.68e-48 163
MS.gene07006.t1 AT5G06420 52.564 156 24 1 146 251 129 284 4.85e-46 158
MS.gene07006.t1 AT5G06420 52.564 156 24 1 146 251 129 284 4.85e-46 158
MS.gene07006.t1 AT1G01350 51.923 156 25 1 146 251 94 249 5.37e-45 154

Find 62 sgRNAs with CRISPR-Local

Find 90 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGACGTACTTTGGGATGATT+TGG 0.280115 7.2:+5263289 None:intergenic
AACTCTATCCAAACTAATTT+CGG 0.290124 7.2:-5263327 MS.gene07006:CDS
GAACAAGAGCTTAGAATATT+GGG 0.292886 7.2:+5263001 None:intergenic
GCGTTTAACGTTGTTGGGTA+TGG 0.309545 7.2:+5263362 None:intergenic
AGAACAAGAGCTTAGAATAT+TGG 0.313825 7.2:+5263000 None:intergenic
TGAAGACTCAAAATGAAAGT+TGG 0.349810 7.2:+5262478 None:intergenic
TTTCTATTATCAATGCAAAT+AGG 0.350139 7.2:-5262577 MS.gene07006:CDS
AATCGTCACCGAAATTAGTT+TGG 0.355363 7.2:+5263319 None:intergenic
ACCGAAGCGTTTAACGTTGT+TGG 0.380689 7.2:+5263356 None:intergenic
AAAATGAAAGTTGGGCTTCT+CGG 0.382812 7.2:+5262487 None:intergenic
TTGAAGTCTTTGCATATGTC+AGG 0.387439 7.2:+5262308 None:intergenic
GTTCTTCAGATGGTACTATC+AGG 0.389104 7.2:-5262982 MS.gene07006:CDS
GAAGACTCAAAATGAAAGTT+GGG 0.391037 7.2:+5262479 None:intergenic
CCAGTGGTGGGTCAATATTA+TGG 0.397482 7.2:-5263082 MS.gene07006:CDS
TCAGCGCACCCTCCTCTTCT+CGG 0.409916 7.2:+5262187 None:intergenic
TGACATATGCAAAGACTTCA+AGG 0.411330 7.2:-5262306 MS.gene07006:CDS
CCGAAGCGTTTAACGTTGTT+GGG 0.411456 7.2:+5263357 None:intergenic
ATTCATCATCAAAGAGATCA+TGG 0.423985 7.2:+5262553 None:intergenic
CGTTTAACGTTGTTGGGTAT+GGG 0.432856 7.2:+5263363 None:intergenic
GACCGAGGCGACTACAAGTC+CGG 0.435443 7.2:-5262239 MS.gene07006:CDS
TAGCAAAGCAACAGCAACTT+TGG 0.436558 7.2:-5262432 MS.gene07006:CDS
GGACACAAGAACATTTCAAC+AGG 0.464750 7.2:-5262954 MS.gene07006:intron
ACGTTGTTGGGTATGGGAAA+AGG 0.467623 7.2:+5263369 None:intergenic
TTGTGAATGGAAGGGATGAT+TGG 0.474743 7.2:-5263153 MS.gene07006:intron
AATGGAAGCAATTGCGGCCA+TGG 0.476431 7.2:-5263409 MS.gene07006:CDS
TGCAAAGACTTCAAGGAGAC+CGG 0.477457 7.2:-5262299 MS.gene07006:CDS
CTAAGCTCTTGTTCTTCAGA+TGG 0.477643 7.2:-5262992 MS.gene07006:CDS
CAGATGGTACTATCAGGACT+TGG 0.481372 7.2:-5262976 MS.gene07006:CDS
GGTGGCAGTTGGACAAAGAA+TGG 0.496757 7.2:-5262217 MS.gene07006:CDS
CCCAACAACGTTAAACGCTT+CGG 0.516028 7.2:-5263357 MS.gene07006:CDS
CCATAATATTGACCCACCAC+TGG 0.537095 7.2:+5263082 None:intergenic
GAAACTGTATTCACCAGTGG+TGG 0.542400 7.2:-5263095 MS.gene07006:CDS
ACAATAAATGGAAGCAATTG+CGG 0.543794 7.2:-5263415 None:intergenic
TTCAAGGAGACCGGGTATTG+TGG 0.545327 7.2:-5262290 MS.gene07006:CDS
ACCCGGACTTGTAGTCGCCT+CGG 0.548237 7.2:+5262237 None:intergenic
AGAAGAGGAGGGTGCGCTGA+TGG 0.548558 7.2:-5262184 None:intergenic
TTCTTTGTCCAACTGCCACC+CGG 0.548782 7.2:+5262220 None:intergenic
AAGGGATGATTGGAGTTCAA+TGG 0.555515 7.2:-5263143 MS.gene07006:CDS
GGAATGAAGCCGAGAAGAGG+AGG 0.558808 7.2:-5262196 MS.gene07006:CDS
TCAATATTATGGAGAGTGCA+AGG 0.560776 7.2:-5263071 MS.gene07006:CDS
TGCAAGTTTCTTCACGACCG+AGG 0.564969 7.2:-5262254 MS.gene07006:CDS
CTACAAGTCCGGGTGGCAGT+TGG 0.571560 7.2:-5262228 MS.gene07006:CDS
ACCGAGGCGACTACAAGTCC+GGG 0.574409 7.2:-5262238 MS.gene07006:CDS
GAGACCGGGTATTGTGGCTA+CGG 0.578795 7.2:-5262284 MS.gene07006:CDS
ATCGCCGTAGCCACAATACC+CGG 0.586901 7.2:+5262280 None:intergenic
AATGGAATGAAGCCGAGAAG+AGG 0.595307 7.2:-5262199 MS.gene07006:CDS
GAAACTCTAATATGAGTCGA+AGG 0.595574 7.2:+5262350 None:intergenic
TCGTTGTTCCTCGACATCCA+TGG 0.607781 7.2:+5263392 None:intergenic
TTCATCATCAAAGAGATCAT+GGG 0.607963 7.2:+5262554 None:intergenic
AGATGGTACTATCAGGACTT+GGG 0.627841 7.2:-5262975 MS.gene07006:CDS
GCAAAGACTTCAAGGAGACC+GGG 0.633254 7.2:-5262298 MS.gene07006:CDS
AATTGCGGCCATGGATGTCG+AGG 0.639683 7.2:-5263400 MS.gene07006:CDS
GAATGAAGCCGAGAAGAGGA+GGG 0.646762 7.2:-5262195 MS.gene07006:CDS
GGATGATTGGAGTTCAATGG+CGG 0.648745 7.2:-5263140 MS.gene07006:CDS
ATCCGTGGGTCATACTCACA+CGG 0.649413 7.2:-5262383 MS.gene07006:CDS
GTGTGAGTATGACCCACGGA+TGG 0.652577 7.2:+5262385 None:intergenic
AAACTGTATTCACCAGTGGT+GGG 0.659751 7.2:-5263094 MS.gene07006:CDS
CAATATTATGGAGAGTGCAA+GGG 0.660822 7.2:-5263070 MS.gene07006:CDS
GACCGTGTGAGTATGACCCA+CGG 0.674066 7.2:+5262381 None:intergenic
AGTGAAACTGTATTCACCAG+TGG 0.687068 7.2:-5263098 MS.gene07006:CDS
ATATGAGTCGAAGGTCTGAG+AGG 0.697705 7.2:+5262359 None:intergenic
GAGGCGACTACAAGTCCGGG+TGG 0.737253 7.2:-5262235 MS.gene07006:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTGTTACTAATGTTATTATT+TGG - chr7.2:5262675-5262694 MS.gene07006:intron 15.0%
!!! AATTAAGTTGTTTATCTATA+AGG + chr7.2:5262725-5262744 None:intergenic 15.0%
!! ATTATAAATCTCCCAAATTA+AGG + chr7.2:5262410-5262429 None:intergenic 20.0%
!! TTATAAATCTCCCAAATTAA+GGG + chr7.2:5262409-5262428 None:intergenic 20.0%
!! TTTATTTATGCCCTTAATTT+GGG - chr7.2:5262396-5262415 MS.gene07006:CDS 20.0%
!! TTTCTATTATCAATGCAAAT+AGG - chr7.2:5263018-5263037 MS.gene07006:CDS 20.0%
!!! TATCAATCAGATTTTGTTTT+CGG - chr7.2:5262560-5262579 MS.gene07006:CDS 20.0%
!!! TTGGGAGATTTATAATTTTT+GGG - chr7.2:5262414-5262433 MS.gene07006:CDS 20.0%
!!! TTTGGGAGATTTATAATTTT+TGG - chr7.2:5262413-5262432 MS.gene07006:CDS 20.0%
!!! TTTTATTTATGCCCTTAATT+TGG - chr7.2:5262395-5262414 MS.gene07006:CDS 20.0%
! AACTCTATCCAAACTAATTT+CGG - chr7.2:5262268-5262287 MS.gene07006:CDS 25.0%
! TCAAATAAGTCAATGCAAAT+AGG - chr7.2:5262944-5262963 MS.gene07006:intron 25.0%
!! GTTTTTCTCTCCATAATTAT+TGG - chr7.2:5262697-5262716 MS.gene07006:intron 25.0%
!!! ATCAATCAGATTTTGTTTTC+GGG - chr7.2:5262561-5262580 MS.gene07006:CDS 25.0%
!!! TTTTTGGGTTTTTTTGTGAA+TGG - chr7.2:5262429-5262448 MS.gene07006:CDS 25.0%
AAATTGAATGTCAAAACTGC+AGG - chr7.2:5262995-5263014 MS.gene07006:CDS 30.0%
ACTTATTTGAGCTTATCTAG+TGG + chr7.2:5262934-5262953 None:intergenic 30.0%
AGAACAAGAGCTTAGAATAT+TGG + chr7.2:5262598-5262617 None:intergenic 30.0%
AGCTTAAGAAGAAAGTGAAA+AGG + chr7.2:5262330-5262349 None:intergenic 30.0%
ATAAGCACGTATATGATTAG+TGG + chr7.2:5262765-5262784 None:intergenic 30.0%
ATTCATCATCAAAGAGATCA+TGG + chr7.2:5263045-5263064 None:intergenic 30.0%
GAACAAGAGCTTAGAATATT+GGG + chr7.2:5262597-5262616 None:intergenic 30.0%
GAAGACTCAAAATGAAAGTT+GGG + chr7.2:5263119-5263138 None:intergenic 30.0%
TGAAGACTCAAAATGAAAGT+TGG + chr7.2:5263120-5263139 None:intergenic 30.0%
TGAGAAAAAGAACATGAAGA+AGG - chr7.2:5263067-5263086 MS.gene07006:CDS 30.0%
TTCATCATCAAAGAGATCAT+GGG + chr7.2:5263044-5263063 None:intergenic 30.0%
! CTATAAGGCACCAATAATTA+TGG + chr7.2:5262710-5262729 None:intergenic 30.0%
AAAATGAAAGTTGGGCTTCT+CGG + chr7.2:5263111-5263130 None:intergenic 35.0%
AAAGTGAAAAGGACGTACTT+TGG + chr7.2:5262319-5262338 None:intergenic 35.0%
AAGTGAAAAGGACGTACTTT+GGG + chr7.2:5262318-5262337 None:intergenic 35.0%
AATCGTCACCGAAATTAGTT+TGG + chr7.2:5262279-5262298 None:intergenic 35.0%
CTTATTTCCATAAGCTCTTG+AGG + chr7.2:5262856-5262875 None:intergenic 35.0%
GAAACTCTAATATGAGTCGA+AGG + chr7.2:5263248-5263267 None:intergenic 35.0%
TGACATATGCAAAGACTTCA+AGG - chr7.2:5263289-5263308 MS.gene07006:intron 35.0%
TTGAAGTCTTTGCATATGTC+AGG + chr7.2:5263290-5263309 None:intergenic 35.0%
! CAATATTATGGAGAGTGCAA+GGG - chr7.2:5262525-5262544 MS.gene07006:CDS 35.0%
! TCAATATTATGGAGAGTGCA+AGG - chr7.2:5262524-5262543 MS.gene07006:CDS 35.0%
!!! ATTTTTCAAGTTCCATCCGT+GGG - chr7.2:5263198-5263217 MS.gene07006:intron 35.0%
!!! GGGTTTTTTTGTGAATGGAA+GGG - chr7.2:5262434-5262453 MS.gene07006:CDS 35.0%
!!! TGGGTTTTTTTGTGAATGGA+AGG - chr7.2:5262433-5262452 MS.gene07006:CDS 35.0%
AAACTGTATTCACCAGTGGT+GGG - chr7.2:5262501-5262520 MS.gene07006:CDS 40.0%
AAGGGATGATTGGAGTTCAA+TGG - chr7.2:5262452-5262471 MS.gene07006:CDS 40.0%
AGATGGTACTATCAGGACTT+GGG - chr7.2:5262620-5262639 MS.gene07006:intron 40.0%
AGTGAAACTGTATTCACCAG+TGG - chr7.2:5262497-5262516 MS.gene07006:CDS 40.0%
CGTTTAACGTTGTTGGGTAT+GGG + chr7.2:5262235-5262254 None:intergenic 40.0%
CTAAGCTCTTGTTCTTCAGA+TGG - chr7.2:5262603-5262622 MS.gene07006:intron 40.0%
GGACACAAGAACATTTCAAC+AGG - chr7.2:5262641-5262660 MS.gene07006:intron 40.0%
GTTCTTCAGATGGTACTATC+AGG - chr7.2:5262613-5262632 MS.gene07006:intron 40.0%
TAGCAAAGCAACAGCAACTT+TGG - chr7.2:5263163-5263182 MS.gene07006:intron 40.0%
TCAAGCAGCAAAGAGATCAT+GGG + chr7.2:5262970-5262989 None:intergenic 40.0%
TTGTGAATGGAAGGGATGAT+TGG - chr7.2:5262442-5262461 MS.gene07006:CDS 40.0%
! CTGAGGATAGCTTGTGAAAA+CGG + chr7.2:5262832-5262851 None:intergenic 40.0%
!!! CATTTTTCAAGTTCCATCCG+TGG - chr7.2:5263197-5263216 MS.gene07006:intron 40.0%
AATGGAATGAAGCCGAGAAG+AGG - chr7.2:5263396-5263415 MS.gene07006:CDS 45.0%
ACGTTGTTGGGTATGGGAAA+AGG + chr7.2:5262229-5262248 None:intergenic 45.0%
ATATGAGTCGAAGGTCTGAG+AGG + chr7.2:5263239-5263258 None:intergenic 45.0%
CAGATGGTACTATCAGGACT+TGG - chr7.2:5262619-5262638 MS.gene07006:intron 45.0%
CCATAATATTGACCCACCAC+TGG + chr7.2:5262516-5262535 None:intergenic 45.0%
CCCAACAACGTTAAACGCTT+CGG - chr7.2:5262238-5262257 MS.gene07006:CDS 45.0%
CTCAAGCAGCAAAGAGATCA+TGG + chr7.2:5262971-5262990 None:intergenic 45.0%
GAAACTGTATTCACCAGTGG+TGG - chr7.2:5262500-5262519 MS.gene07006:CDS 45.0%
GCGTTTAACGTTGTTGGGTA+TGG + chr7.2:5262236-5262255 None:intergenic 45.0%
GGATGATTGGAGTTCAATGG+CGG - chr7.2:5262455-5262474 MS.gene07006:CDS 45.0%
TGCAAAGACTTCAAGGAGAC+CGG - chr7.2:5263296-5263315 MS.gene07006:intron 45.0%
! ACCGAAGCGTTTAACGTTGT+TGG + chr7.2:5262242-5262261 None:intergenic 45.0%
! CCGAAGCGTTTAACGTTGTT+GGG + chr7.2:5262241-5262260 None:intergenic 45.0%
!! CCAGTGGTGGGTCAATATTA+TGG - chr7.2:5262513-5262532 MS.gene07006:CDS 45.0%
!! GGACGTACTTTGGGATGATT+TGG + chr7.2:5262309-5262328 None:intergenic 45.0%
ATCCGTGGGTCATACTCACA+CGG - chr7.2:5263212-5263231 MS.gene07006:intron 50.0%
CCATAAGCTCTTGAGGTCTG+AGG + chr7.2:5262849-5262868 None:intergenic 50.0%
CCTCAGACCTCAAGAGCTTA+TGG - chr7.2:5262846-5262865 MS.gene07006:intron 50.0%
GAATGAAGCCGAGAAGAGGA+GGG - chr7.2:5263400-5263419 MS.gene07006:CDS 50.0%
GCAAAGACTTCAAGGAGACC+GGG - chr7.2:5263297-5263316 MS.gene07006:CDS 50.0%
GGTGGCAGTTGGACAAAGAA+TGG - chr7.2:5263378-5263397 MS.gene07006:CDS 50.0%
TCGTTGTTCCTCGACATCCA+TGG + chr7.2:5262206-5262225 None:intergenic 50.0%
TGCAAGTTTCTTCACGACCG+AGG - chr7.2:5263341-5263360 MS.gene07006:CDS 50.0%
TTCTTTGTCCAACTGCCACC+CGG + chr7.2:5263378-5263397 None:intergenic 50.0%
! TTCAAGGAGACCGGGTATTG+TGG - chr7.2:5263305-5263324 MS.gene07006:CDS 50.0%
AATTGCGGCCATGGATGTCG+AGG - chr7.2:5262195-5262214 MS.gene07006:CDS 55.0%
ATCGCCGTAGCCACAATACC+CGG + chr7.2:5263318-5263337 None:intergenic 55.0%
GACCGTGTGAGTATGACCCA+CGG + chr7.2:5263217-5263236 None:intergenic 55.0%
GGAATGAAGCCGAGAAGAGG+AGG - chr7.2:5263399-5263418 MS.gene07006:CDS 55.0%
GTGTGAGTATGACCCACGGA+TGG + chr7.2:5263213-5263232 None:intergenic 55.0%
! GAGACCGGGTATTGTGGCTA+CGG - chr7.2:5263311-5263330 MS.gene07006:CDS 55.0%
ACCCGGACTTGTAGTCGCCT+CGG + chr7.2:5263361-5263380 None:intergenic 60.0%
ACCGAGGCGACTACAAGTCC+GGG - chr7.2:5263357-5263376 MS.gene07006:CDS 60.0%
CTACAAGTCCGGGTGGCAGT+TGG - chr7.2:5263367-5263386 MS.gene07006:CDS 60.0%
GACCGAGGCGACTACAAGTC+CGG - chr7.2:5263356-5263375 MS.gene07006:CDS 60.0%
TCAGCGCACCCTCCTCTTCT+CGG + chr7.2:5263411-5263430 None:intergenic 60.0%
GAGGCGACTACAAGTCCGGG+TGG - chr7.2:5263360-5263379 MS.gene07006:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 5262187 5263430 5262187 ID=MS.gene07006
chr7.2 mRNA 5262187 5263430 5262187 ID=MS.gene07006.t1;Parent=MS.gene07006
chr7.2 exon 5263297 5263430 5263297 ID=MS.gene07006.t1.exon1;Parent=MS.gene07006.t1
chr7.2 CDS 5263297 5263430 5263297 ID=cds.MS.gene07006.t1;Parent=MS.gene07006.t1
chr7.2 exon 5262955 5263162 5262955 ID=MS.gene07006.t1.exon2;Parent=MS.gene07006.t1
chr7.2 CDS 5262955 5263162 5262955 ID=cds.MS.gene07006.t1;Parent=MS.gene07006.t1
chr7.2 exon 5262187 5262600 5262187 ID=MS.gene07006.t1.exon3;Parent=MS.gene07006.t1
chr7.2 CDS 5262187 5262600 5262187 ID=cds.MS.gene07006.t1;Parent=MS.gene07006.t1
Gene Sequence

>MS.gene07006

ATGGAAGCAATTGCGGCCATGGATGTCGAGGAACAACGACCTTTTCCCATACCCAACAACGTTAAACGCTTCGGTCTTAAAAACTCTATCCAAACTAATTTCGGTGACGATTATGTTTTCCAAATCATCCCAAAGTACGTCCTTTTCACTTTCTTCTTAAGCTTTTCAATTATACTCAAGCTTGATCAAATATTGAAAAAGTTCACTTTTTTATTTATGCCCTTAATTTGGGAGATTTATAATTTTTGGGTTTTTTTGTGAATGGAAGGGATGATTGGAGTTCAATGGCGGTTTCACTTTCGACGAACGCAGTGAAACTGTATTCACCAGTGGTGGGTCAATATTATGGAGAGTGCAAGGGTCACACTGCAACTATCAATCAGATTTTGTTTTCGGGTCATTCTAATCCCAATATTCTAAGCTCTTGTTCTTCAGATGGTACTATCAGGACTTGGGACACAAGAACATTTCAACAGGTTGATTTTTTGTTGTTACTAATGTTATTATTTGGTTTTTCTCTCCATAATTATTGGTGCCTTATAGATAAACAACTTAATTAAGCGTTTATAGCACAACCACTAATCATATACGTGCTTATTTCTATAACAAATGACAAAATAAAGCTAAATTGTTTCATACAAGCCGTTTTCACAAGCTATCCTCAGACCTCAAGAGCTTATGGAAATAAGCTGAATCAAATATTTTGTCAGAAACTCTCTTGAATAGTCTCACAAAGTGTCTATGCCACTAGATAAGCTCAAATAAGTCAATGCAAATAGGCCCATGATCTCTTTGCTGCTTGAGTGTTAAATTGAATGTCAAAACTGCAGGTTTCTATTATCAATGCAAATAGGCCCATGATCTCTTTGATGATGAATGTTGAGAAAAAGAACATGAAGAAGGCTGACAGTAATGAAGAATCCGAGAAGCCCAACTTTCATTTTGAGTCTTCAAAAGAGATTCAAGTTCAACATGATAGCAAAGCAACAGCAACTTTGGAGACTGAGACTCATTTTTCAAGTTCCATCCGTGGGTCATACTCACACGGTCCTCTCAGACCTTCGACTCATATTAGAGTTTCATCAAGAATCGATTATCAACCTGACATATGCAAAGACTTCAAGGAGACCGGGTATTGTGGCTACGGCGATTCATGCAAGTTTCTTCACGACCGAGGCGACTACAAGTCCGGGTGGCAGTTGGACAAAGAATGGAATGAAGCCGAGAAGAGGAGGGTGCGCTGA

Protein sequence

>MS.gene07006.t1

MEAIAAMDVEEQRPFPIPNNVKRFGLKNSIQTNFGDDYVFQIIPKDDWSSMAVSLSTNAVKLYSPVVGQYYGECKGHTATINQILFSGHSNPNILSSCSSDGTIRTWDTRTFQQVSIINANRPMISLMMNVEKKNMKKADSNEESEKPNFHFESSKEIQVQHDSKATATLETETHFSSSIRGSYSHGPLRPSTHIRVSSRIDYQPDICKDFKETGYCGYGDSCKFLHDRGDYKSGWQLDKEWNEAEKRRVR