Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07007.t1 | KEH19403.1 | 69.3 | 137 | 34 | 2 | 54 | 190 | 18 | 146 | 1.30E-43 | 186.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07007.t1 | Q9FNG6 | 40.8 | 223 | 66 | 4 | 3 | 160 | 57 | 278 | 2.4e-35 | 150.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07007.t1 | A0A072U5J1 | 69.3 | 137 | 34 | 2 | 54 | 190 | 18 | 146 | 9.0e-44 | 186.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene07007.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07007.t1 | MTR_7g108868 | 69.343 | 137 | 34 | 2 | 54 | 190 | 18 | 146 | 5.65e-62 | 191 |
| MS.gene07007.t1 | MTR_8g058593 | 69.343 | 137 | 34 | 2 | 54 | 190 | 18 | 146 | 5.65e-62 | 191 |
| MS.gene07007.t1 | MTR_8g058607 | 79.279 | 111 | 21 | 1 | 54 | 164 | 31 | 139 | 4.26e-60 | 184 |
| MS.gene07007.t1 | MTR_7g109250 | 45.833 | 240 | 57 | 4 | 1 | 167 | 1 | 240 | 1.36e-57 | 184 |
| MS.gene07007.t1 | MTR_7g109250 | 45.833 | 240 | 57 | 4 | 1 | 167 | 61 | 300 | 1.57e-56 | 183 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07007.t1 | AT5G06420 | 42.060 | 233 | 69 | 4 | 3 | 170 | 57 | 288 | 1.39e-51 | 170 |
| MS.gene07007.t1 | AT5G06420 | 42.060 | 233 | 69 | 4 | 3 | 170 | 57 | 288 | 1.39e-51 | 170 |
| MS.gene07007.t1 | AT1G01350 | 39.130 | 253 | 71 | 4 | 1 | 170 | 1 | 253 | 8.69e-51 | 167 |
Find 36 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGAAGGGAAAAGGACTTTC+TGG | 0.207237 | 7.2:-5264798 | MS.gene07007:CDS |
| AAAATGAAAGATGTGCTTCT+TGG | 0.227049 | 7.2:+5265159 | None:intergenic |
| GCGCACACTCTTTGCTTTCT+CGG | 0.276894 | 7.2:+5264859 | None:intergenic |
| GACCGAGGCGACTACAATTC+CGG | 0.339363 | 7.2:-5264911 | MS.gene07007:CDS |
| TTGAAGTCTTTGCATATGTC+AGG | 0.387439 | 7.2:+5264980 | None:intergenic |
| TGACATATGCAAAGACTTCA+AGG | 0.411330 | 7.2:-5264978 | MS.gene07007:CDS |
| ACCGAGGCGACTACAATTCC+GGG | 0.427387 | 7.2:-5264910 | MS.gene07007:CDS |
| TGTGGGTTGTTTAGAAAAGT+TGG | 0.448560 | 7.2:-5265298 | MS.gene07007:CDS |
| ACACACTGAAGAAGCAGGTT+TGG | 0.459341 | 7.2:+5265318 | None:intergenic |
| TGCAAAGACTTCAAGGAGAC+CGG | 0.477457 | 7.2:-5264971 | MS.gene07007:CDS |
| TAGCAAAGCAACATCAACTT+TGG | 0.480022 | 7.2:-5265104 | MS.gene07007:CDS |
| AACCTGCTTCTTCAGTGTGT+GGG | 0.483768 | 7.2:-5265315 | MS.gene07007:CDS |
| AAACCTGCTTCTTCAGTGTG+TGG | 0.491141 | 7.2:-5265316 | MS.gene07007:CDS |
| TGAGAATGAAGAAGATAATG+AGG | 0.496162 | 7.2:-5265245 | MS.gene07007:CDS |
| GGTGGCAGTTGGACAAAGAA+TGG | 0.496757 | 7.2:-5264889 | MS.gene07007:CDS |
| AAAGAGTGTGCGCGCGCTGA+TGG | 0.507062 | 7.2:-5264849 | MS.gene07007:CDS |
| TTGTCTGTTTGTTTGTCTGA+AGG | 0.512605 | 7.2:-5264825 | MS.gene07007:CDS |
| TTCAAGGAGACCGGGTATTG+TGG | 0.545327 | 7.2:-5264962 | MS.gene07007:CDS |
| CTACAATTCCGGGTGGCAGT+TGG | 0.548426 | 7.2:-5264900 | MS.gene07007:CDS |
| ACCCGGAATTGTAGTCGCCT+CGG | 0.550971 | 7.2:+5264909 | None:intergenic |
| TTCTTTGTCCAACTGCCACC+CGG | 0.562902 | 7.2:+5264892 | None:intergenic |
| TGCAAGTTTCTTCACGACCG+AGG | 0.564969 | 7.2:-5264926 | MS.gene07007:CDS |
| GAGACCGGGTATTGTGGCTA+CGG | 0.578795 | 7.2:-5264956 | MS.gene07007:CDS |
| TTGGTGCTAAGAAGAATTTG+AGG | 0.580649 | 7.2:-5265279 | MS.gene07007:CDS |
| ATCGCCGTAGCCACAATACC+CGG | 0.586901 | 7.2:+5264952 | None:intergenic |
| AACCCACACACTGAAGAAGC+AGG | 0.595301 | 7.2:+5265313 | None:intergenic |
| GAAACTCTAATATGAGTCGA+AGG | 0.595574 | 7.2:+5265022 | None:intergenic |
| TGTTGAGAAGAACATGAAGA+AGG | 0.607739 | 7.2:-5265200 | MS.gene07007:CDS |
| TAGTATGGAAGATTCTTCTG+AGG | 0.619198 | 7.2:-5265341 | None:intergenic |
| ACATGTAAATTACAAACACC+CGG | 0.625741 | 7.2:+5264757 | None:intergenic |
| GCAAAGACTTCAAGGAGACC+GGG | 0.633254 | 7.2:-5264970 | MS.gene07007:CDS |
| ATCCGTGGGTCATACTCACA+CGG | 0.649413 | 7.2:-5265055 | MS.gene07007:CDS |
| GTGTGAGTATGACCCACGGA+TGG | 0.652577 | 7.2:+5265057 | None:intergenic |
| GACCGTGTGAGTATGACCCA+CGG | 0.674066 | 7.2:+5265053 | None:intergenic |
| ATATGAGTCGAAGGTCTGAG+AGG | 0.697705 | 7.2:+5265031 | None:intergenic |
| GAGGCGACTACAATTCCGGG+TGG | 0.758896 | 7.2:-5264907 | MS.gene07007:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATGAAAGATGTGCTTCT+TGG | + | chr7.2:5264947-5264966 | None:intergenic | 30.0% | |
| TGAGAATGAAGAAGATAATG+AGG | - | chr7.2:5264858-5264877 | MS.gene07007:CDS | 30.0% | |
| TTGTCTGAAGGAAAAAAGAA+GGG | - | chr7.2:5265290-5265309 | MS.gene07007:CDS | 30.0% | |
| TTTGTCTGAAGGAAAAAAGA+AGG | - | chr7.2:5265289-5265308 | MS.gene07007:CDS | 30.0% | |
| GAAACTCTAATATGAGTCGA+AGG | + | chr7.2:5265084-5265103 | None:intergenic | 35.0% | |
| GAAGGAAAAAAGAAGGGAAA+AGG | - | chr7.2:5265296-5265315 | MS.gene07007:CDS | 35.0% | |
| TAGCAAAGCAACATCAACTT+TGG | - | chr7.2:5264999-5265018 | MS.gene07007:CDS | 35.0% | |
| TGACATATGCAAAGACTTCA+AGG | - | chr7.2:5265125-5265144 | MS.gene07007:CDS | 35.0% | |
| TGTTGAGAAGAACATGAAGA+AGG | - | chr7.2:5264903-5264922 | MS.gene07007:CDS | 35.0% | |
| TTGAAGTCTTTGCATATGTC+AGG | + | chr7.2:5265126-5265145 | None:intergenic | 35.0% | |
| TTGTCTGTTTGTTTGTCTGA+AGG | - | chr7.2:5265278-5265297 | MS.gene07007:CDS | 35.0% | |
| ! | TGTGGGTTGTTTAGAAAAGT+TGG | - | chr7.2:5264805-5264824 | MS.gene07007:CDS | 35.0% |
| !! | ATTTTGCTGACTACAATTCC+GGG | - | chr7.2:5265328-5265347 | MS.gene07007:CDS | 35.0% |
| !! | GATTTTGCTGACTACAATTC+CGG | - | chr7.2:5265327-5265346 | MS.gene07007:CDS | 35.0% |
| !! | TTGGTGCTAAGAAGAATTTG+AGG | - | chr7.2:5264824-5264843 | MS.gene07007:CDS | 35.0% |
| !!! | ATTTTTCAAGTTCCATCCGT+GGG | - | chr7.2:5265034-5265053 | MS.gene07007:CDS | 35.0% |
| AAGAAGGGAAAAGGACTTTC+TGG | - | chr7.2:5265305-5265324 | MS.gene07007:CDS | 40.0% | |
| !!! | CATTTTTCAAGTTCCATCCG+TGG | - | chr7.2:5265033-5265052 | MS.gene07007:CDS | 40.0% |
| AAACCTGCTTCTTCAGTGTG+TGG | - | chr7.2:5264787-5264806 | MS.gene07007:CDS | 45.0% | |
| AACCTGCTTCTTCAGTGTGT+GGG | - | chr7.2:5264788-5264807 | MS.gene07007:CDS | 45.0% | |
| ACACACTGAAGAAGCAGGTT+TGG | + | chr7.2:5264788-5264807 | None:intergenic | 45.0% | |
| ATATGAGTCGAAGGTCTGAG+AGG | + | chr7.2:5265075-5265094 | None:intergenic | 45.0% | |
| TGCAAAGACTTCAAGGAGAC+CGG | - | chr7.2:5265132-5265151 | MS.gene07007:CDS | 45.0% | |
| AACCCACACACTGAAGAAGC+AGG | + | chr7.2:5264793-5264812 | None:intergenic | 50.0% | |
| ATCCGTGGGTCATACTCACA+CGG | - | chr7.2:5265048-5265067 | MS.gene07007:CDS | 50.0% | |
| GCAAAGACTTCAAGGAGACC+GGG | - | chr7.2:5265133-5265152 | MS.gene07007:CDS | 50.0% | |
| GCGCACACTCTTTGCTTTCT+CGG | + | chr7.2:5265247-5265266 | None:intergenic | 50.0% | |
| GGTGGCAGTTGGACAAAGAA+TGG | - | chr7.2:5265214-5265233 | MS.gene07007:CDS | 50.0% | |
| TGCAAGTTTCTTCACGACCG+AGG | - | chr7.2:5265177-5265196 | MS.gene07007:CDS | 50.0% | |
| TTCTTTGTCCAACTGCCACC+CGG | + | chr7.2:5265214-5265233 | None:intergenic | 50.0% | |
| ! | TTCAAGGAGACCGGGTATTG+TGG | - | chr7.2:5265141-5265160 | MS.gene07007:CDS | 50.0% |
| ACCCGGAATTGTAGTCGCCT+CGG | + | chr7.2:5265197-5265216 | None:intergenic | 55.0% | |
| ACCGAGGCGACTACAATTCC+GGG | - | chr7.2:5265193-5265212 | MS.gene07007:CDS | 55.0% | |
| ATCGCCGTAGCCACAATACC+CGG | + | chr7.2:5265154-5265173 | None:intergenic | 55.0% | |
| CTACAATTCCGGGTGGCAGT+TGG | - | chr7.2:5265203-5265222 | MS.gene07007:CDS | 55.0% | |
| GACCGAGGCGACTACAATTC+CGG | - | chr7.2:5265192-5265211 | MS.gene07007:CDS | 55.0% | |
| GACCGTGTGAGTATGACCCA+CGG | + | chr7.2:5265053-5265072 | None:intergenic | 55.0% | |
| GTGTGAGTATGACCCACGGA+TGG | + | chr7.2:5265049-5265068 | None:intergenic | 55.0% | |
| ! | GAGACCGGGTATTGTGGCTA+CGG | - | chr7.2:5265147-5265166 | MS.gene07007:CDS | 55.0% |
| GAGGCGACTACAATTCCGGG+TGG | - | chr7.2:5265196-5265215 | MS.gene07007:CDS | 60.0% | |
| ! | AAAGAGTGTGCGCGCGCTGA+TGG | - | chr7.2:5265254-5265273 | MS.gene07007:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 5264766 | 5265359 | 5264766 | ID=MS.gene07007 |
| chr7.2 | mRNA | 5264766 | 5265359 | 5264766 | ID=MS.gene07007.t1;Parent=MS.gene07007 |
| chr7.2 | exon | 5264766 | 5265359 | 5264766 | ID=MS.gene07007.t1.exon1;Parent=MS.gene07007.t1 |
| chr7.2 | CDS | 5264766 | 5265359 | 5264766 | ID=cds.MS.gene07007.t1;Parent=MS.gene07007.t1 |
| Gene Sequence |
| Protein sequence |
