Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07008.t1 | KEH19406.1 | 83.2 | 113 | 17 | 1 | 54 | 166 | 31 | 141 | 3.30E-45 | 191.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07008.t1 | Q9FNG6 | 41.7 | 223 | 64 | 4 | 3 | 160 | 57 | 278 | 2.4e-36 | 153.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07008.t1 | A0A072TQE4 | 83.2 | 113 | 17 | 1 | 54 | 166 | 31 | 141 | 2.4e-45 | 191.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene07008.t1 | TF | C3H |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07008.t1 | MTR_8g058607 | 83.186 | 113 | 17 | 1 | 54 | 166 | 31 | 141 | 5.10e-65 | 196 |
| MS.gene07008.t1 | MTR_7g108868 | 82.883 | 111 | 17 | 1 | 54 | 164 | 18 | 126 | 7.20e-64 | 194 |
| MS.gene07008.t1 | MTR_8g058593 | 82.883 | 111 | 17 | 1 | 54 | 164 | 18 | 126 | 7.20e-64 | 194 |
| MS.gene07008.t1 | MTR_7g109250 | 46.835 | 237 | 53 | 4 | 1 | 164 | 1 | 237 | 5.28e-58 | 184 |
| MS.gene07008.t1 | MTR_7g109250 | 46.835 | 237 | 53 | 4 | 1 | 164 | 61 | 297 | 9.36e-57 | 182 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07008.t1 | AT5G06420 | 44.105 | 229 | 62 | 4 | 3 | 166 | 57 | 284 | 8.71e-54 | 174 |
| MS.gene07008.t1 | AT5G06420 | 44.105 | 229 | 62 | 4 | 3 | 166 | 57 | 284 | 8.71e-54 | 174 |
| MS.gene07008.t1 | AT1G01350 | 40.964 | 249 | 64 | 4 | 1 | 166 | 1 | 249 | 6.49e-53 | 171 |
Find 36 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATGAAAGATGTGCTTCT+TGG | 0.227049 | 7.2:+5266102 | None:intergenic |
| TTGAAGTCTTTGCATATGTC+AGG | 0.387439 | 7.2:+5265923 | None:intergenic |
| TCAGCGCACCCTCCTCTTCT+CGG | 0.409916 | 7.2:+5265802 | None:intergenic |
| TGACATATGCAAAGACTTCA+AGG | 0.411330 | 7.2:-5265921 | MS.gene07008:CDS |
| GACCGAGGCGACTACAAGTC+CGG | 0.435443 | 7.2:-5265854 | MS.gene07008:CDS |
| TAGCAAAGCAACAGCAACTT+TGG | 0.436558 | 7.2:-5266047 | MS.gene07008:CDS |
| TGTGGGTTGTTTAGAAAAGT+TGG | 0.448560 | 7.2:-5266241 | MS.gene07008:CDS |
| ACACACTGAAGAAGCAGGTT+TGG | 0.459341 | 7.2:+5266261 | None:intergenic |
| TGCAAAGACTTCAAGGAGAC+CGG | 0.477457 | 7.2:-5265914 | MS.gene07008:CDS |
| AACCTGCTTCTTCAGTGTGT+GGG | 0.483768 | 7.2:-5266258 | MS.gene07008:CDS |
| AAACCTGCTTCTTCAGTGTG+TGG | 0.491141 | 7.2:-5266259 | MS.gene07008:CDS |
| TGAGAATGAAGAAGATAATG+AGG | 0.496162 | 7.2:-5266188 | MS.gene07008:CDS |
| GGTGGCAGTTGGACAAAGAA+TGG | 0.496757 | 7.2:-5265832 | MS.gene07008:CDS |
| TTCAAGGAGACCGGGTATTG+TGG | 0.545327 | 7.2:-5265905 | MS.gene07008:CDS |
| ACCCGGACTTGTAGTCGCCT+CGG | 0.548237 | 7.2:+5265852 | None:intergenic |
| AGAAGAGGAGGGTGCGCTGA+TGG | 0.548558 | 7.2:-5265799 | None:intergenic |
| TTCTTTGTCCAACTGCCACC+CGG | 0.548782 | 7.2:+5265835 | None:intergenic |
| GGAATGAAGCCGAGAAGAGG+AGG | 0.558808 | 7.2:-5265811 | MS.gene07008:CDS |
| TGCAAGTTTCTTCACGACCG+AGG | 0.564969 | 7.2:-5265869 | MS.gene07008:CDS |
| CTACAAGTCCGGGTGGCAGT+TGG | 0.571560 | 7.2:-5265843 | MS.gene07008:CDS |
| ACCGAGGCGACTACAAGTCC+GGG | 0.574409 | 7.2:-5265853 | MS.gene07008:CDS |
| GAGACCGGGTATTGTGGCTA+CGG | 0.578795 | 7.2:-5265899 | MS.gene07008:CDS |
| TTGGTGCTAAGAAGAATTTG+AGG | 0.580649 | 7.2:-5266222 | MS.gene07008:CDS |
| ATCGCCGTAGCCACAATACC+CGG | 0.586901 | 7.2:+5265895 | None:intergenic |
| AACCCACACACTGAAGAAGC+AGG | 0.595301 | 7.2:+5266256 | None:intergenic |
| AATGGAATGAAGCCGAGAAG+AGG | 0.595307 | 7.2:-5265814 | MS.gene07008:CDS |
| GAAACTCTAATATGAGTCGA+AGG | 0.595574 | 7.2:+5265965 | None:intergenic |
| TGTTGAGAAGAACATGAAGA+AGG | 0.607739 | 7.2:-5266143 | MS.gene07008:CDS |
| TAGTATGGAAGATTCTTCTG+AGG | 0.619198 | 7.2:-5266284 | None:intergenic |
| GCAAAGACTTCAAGGAGACC+GGG | 0.633254 | 7.2:-5265913 | MS.gene07008:CDS |
| GAATGAAGCCGAGAAGAGGA+GGG | 0.646762 | 7.2:-5265810 | MS.gene07008:CDS |
| ATCCGTGGGTCATACTCACA+CGG | 0.649413 | 7.2:-5265998 | MS.gene07008:CDS |
| GTGTGAGTATGACCCACGGA+TGG | 0.652577 | 7.2:+5266000 | None:intergenic |
| GACCGTGTGAGTATGACCCA+CGG | 0.674066 | 7.2:+5265996 | None:intergenic |
| ATATGAGTCGAAGGTCTGAG+AGG | 0.697705 | 7.2:+5265974 | None:intergenic |
| GAGGCGACTACAAGTCCGGG+TGG | 0.737253 | 7.2:-5265850 | MS.gene07008:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATGAAAGATGTGCTTCT+TGG | + | chr7.2:5265983-5266002 | None:intergenic | 30.0% | |
| TGAGAATGAAGAAGATAATG+AGG | - | chr7.2:5265894-5265913 | MS.gene07008:CDS | 30.0% | |
| GAAACTCTAATATGAGTCGA+AGG | + | chr7.2:5266120-5266139 | None:intergenic | 35.0% | |
| TGACATATGCAAAGACTTCA+AGG | - | chr7.2:5266161-5266180 | MS.gene07008:CDS | 35.0% | |
| TGTTGAGAAGAACATGAAGA+AGG | - | chr7.2:5265939-5265958 | MS.gene07008:CDS | 35.0% | |
| TTGAAGTCTTTGCATATGTC+AGG | + | chr7.2:5266162-5266181 | None:intergenic | 35.0% | |
| ! | TGTGGGTTGTTTAGAAAAGT+TGG | - | chr7.2:5265841-5265860 | MS.gene07008:CDS | 35.0% |
| !! | TTGGTGCTAAGAAGAATTTG+AGG | - | chr7.2:5265860-5265879 | MS.gene07008:CDS | 35.0% |
| !!! | ATTTTTCAAGTTCCATCCGT+GGG | - | chr7.2:5266070-5266089 | MS.gene07008:CDS | 35.0% |
| TAGCAAAGCAACAGCAACTT+TGG | - | chr7.2:5266035-5266054 | MS.gene07008:CDS | 40.0% | |
| !!! | CATTTTTCAAGTTCCATCCG+TGG | - | chr7.2:5266069-5266088 | MS.gene07008:CDS | 40.0% |
| AAACCTGCTTCTTCAGTGTG+TGG | - | chr7.2:5265823-5265842 | MS.gene07008:CDS | 45.0% | |
| AACCTGCTTCTTCAGTGTGT+GGG | - | chr7.2:5265824-5265843 | MS.gene07008:CDS | 45.0% | |
| AATGGAATGAAGCCGAGAAG+AGG | - | chr7.2:5266268-5266287 | MS.gene07008:CDS | 45.0% | |
| ACACACTGAAGAAGCAGGTT+TGG | + | chr7.2:5265824-5265843 | None:intergenic | 45.0% | |
| ATATGAGTCGAAGGTCTGAG+AGG | + | chr7.2:5266111-5266130 | None:intergenic | 45.0% | |
| TGCAAAGACTTCAAGGAGAC+CGG | - | chr7.2:5266168-5266187 | MS.gene07008:CDS | 45.0% | |
| AACCCACACACTGAAGAAGC+AGG | + | chr7.2:5265829-5265848 | None:intergenic | 50.0% | |
| ATCCGTGGGTCATACTCACA+CGG | - | chr7.2:5266084-5266103 | MS.gene07008:CDS | 50.0% | |
| GAATGAAGCCGAGAAGAGGA+GGG | - | chr7.2:5266272-5266291 | MS.gene07008:CDS | 50.0% | |
| GCAAAGACTTCAAGGAGACC+GGG | - | chr7.2:5266169-5266188 | MS.gene07008:CDS | 50.0% | |
| GGTGGCAGTTGGACAAAGAA+TGG | - | chr7.2:5266250-5266269 | MS.gene07008:CDS | 50.0% | |
| TGCAAGTTTCTTCACGACCG+AGG | - | chr7.2:5266213-5266232 | MS.gene07008:CDS | 50.0% | |
| TTCTTTGTCCAACTGCCACC+CGG | + | chr7.2:5266250-5266269 | None:intergenic | 50.0% | |
| ! | TTCAAGGAGACCGGGTATTG+TGG | - | chr7.2:5266177-5266196 | MS.gene07008:CDS | 50.0% |
| ATCGCCGTAGCCACAATACC+CGG | + | chr7.2:5266190-5266209 | None:intergenic | 55.0% | |
| GACCGTGTGAGTATGACCCA+CGG | + | chr7.2:5266089-5266108 | None:intergenic | 55.0% | |
| GGAATGAAGCCGAGAAGAGG+AGG | - | chr7.2:5266271-5266290 | MS.gene07008:CDS | 55.0% | |
| GTGTGAGTATGACCCACGGA+TGG | + | chr7.2:5266085-5266104 | None:intergenic | 55.0% | |
| ! | GAGACCGGGTATTGTGGCTA+CGG | - | chr7.2:5266183-5266202 | MS.gene07008:CDS | 55.0% |
| ACCCGGACTTGTAGTCGCCT+CGG | + | chr7.2:5266233-5266252 | None:intergenic | 60.0% | |
| ACCGAGGCGACTACAAGTCC+GGG | - | chr7.2:5266229-5266248 | MS.gene07008:CDS | 60.0% | |
| CTACAAGTCCGGGTGGCAGT+TGG | - | chr7.2:5266239-5266258 | MS.gene07008:CDS | 60.0% | |
| GACCGAGGCGACTACAAGTC+CGG | - | chr7.2:5266228-5266247 | MS.gene07008:CDS | 60.0% | |
| TCAGCGCACCCTCCTCTTCT+CGG | + | chr7.2:5266283-5266302 | None:intergenic | 60.0% | |
| GAGGCGACTACAAGTCCGGG+TGG | - | chr7.2:5266232-5266251 | MS.gene07008:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 5265802 | 5266302 | 5265802 | ID=MS.gene07008 |
| chr7.2 | mRNA | 5265802 | 5266302 | 5265802 | ID=MS.gene07008.t1;Parent=MS.gene07008 |
| chr7.2 | exon | 5265802 | 5266302 | 5265802 | ID=MS.gene07008.t1.exon1;Parent=MS.gene07008.t1 |
| chr7.2 | CDS | 5265802 | 5266302 | 5265802 | ID=cds.MS.gene07008.t1;Parent=MS.gene07008.t1 |
| Gene Sequence |
| Protein sequence |
