Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070100.t1 | XP_003612621.2 | 93.5 | 260 | 17 | 0 | 1 | 260 | 1 | 260 | 5.00E-139 | 503.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070100.t1 | Q9ZUM9 | 47.7 | 260 | 120 | 6 | 8 | 259 | 11 | 262 | 8.3e-58 | 225.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070100.t1 | G7K6V9 | 93.5 | 260 | 17 | 0 | 1 | 260 | 1 | 260 | 3.6e-139 | 503.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene070100.t1 | TR | SET |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070100.t1 | MTR_5g027020 | 93.462 | 260 | 17 | 0 | 1 | 260 | 1 | 260 | 0.0 | 505 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070100.t1 | AT2G19640 | 47.692 | 260 | 120 | 6 | 8 | 259 | 11 | 262 | 3.65e-73 | 228 |
| MS.gene070100.t1 | AT2G19640 | 47.692 | 260 | 120 | 6 | 8 | 259 | 11 | 262 | 6.26e-72 | 226 |
Find 61 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTAAATTCCATAAGCCTTAA+TGG | 0.186663 | 5.4:-17894522 | None:intergenic |
| GGGATTCAGTGAGGATTATT+TGG | 0.209184 | 5.4:-17893992 | None:intergenic |
| CAAAGATCCATTAAGGCTTA+TGG | 0.268080 | 5.4:+17894515 | MS.gene070100:CDS |
| CTGAAGCAGTGGTGACAATA+AGG | 0.269041 | 5.4:-17894062 | None:intergenic |
| TGGATCTTTGAGGACCCTTT+TGG | 0.288986 | 5.4:-17894502 | None:intergenic |
| CAAACTTTACTTTCACTTCA+TGG | 0.305377 | 5.4:+17894320 | MS.gene070100:CDS |
| TTCATGTGGGGTGGACAAAA+AGG | 0.320238 | 5.4:-17894404 | None:intergenic |
| GGTGCAAGATTGAAGCTAAT+TGG | 0.320639 | 5.4:+17894774 | MS.gene070100:intron |
| TAATCAAACCTGCATGCATT+AGG | 0.329362 | 5.4:-17894578 | None:intergenic |
| TGAGATCTTGTGTTGCAATA+AGG | 0.333963 | 5.4:-17893962 | None:intergenic |
| AAACTTAGCATTATCAACTA+TGG | 0.347178 | 5.4:-17894363 | None:intergenic |
| ATAAGGCCTCTACCTTTGTT+AGG | 0.358422 | 5.4:-17893945 | None:intergenic |
| GGGTCCTCAAAGATCCATTA+AGG | 0.360632 | 5.4:+17894508 | MS.gene070100:CDS |
| TTCAACCTCTCTCTTGAGAT+TGG | 0.374327 | 5.4:+17893914 | MS.gene070100:CDS |
| ACTATGGAATCATGATCATT+AGG | 0.397254 | 5.4:-17894347 | None:intergenic |
| AGTAAAGTTTGAAGCTTTGA+AGG | 0.405277 | 5.4:-17894308 | None:intergenic |
| AATCAAACCTGCATGCATTA+GGG | 0.410331 | 5.4:-17894577 | None:intergenic |
| AATGGAACCTTATTCCCAAA+AGG | 0.424559 | 5.4:+17894487 | MS.gene070100:CDS |
| GTGAGAGGAATTGAGGGCAT+TGG | 0.448917 | 5.4:-17894159 | None:intergenic |
| ATTGAAGCTAATTGGAATGA+TGG | 0.455273 | 5.4:+17894782 | MS.gene070100:CDS |
| TGCATGCAGGTTTGATTATG+TGG | 0.462661 | 5.4:+17894583 | MS.gene070100:CDS |
| ATGGAACCTTATTCCCAAAA+GGG | 0.489171 | 5.4:+17894488 | MS.gene070100:CDS |
| TCAATTCCTCTCACTCATCT+TGG | 0.489766 | 5.4:+17894168 | MS.gene070100:CDS |
| TAGCAGAATAGAGAAGAAGA+GGG | 0.491920 | 5.4:-17894013 | None:intergenic |
| AAGATGAGTGAGAGGAATTG+AGG | 0.492387 | 5.4:-17894166 | None:intergenic |
| AGATGAGTGAGAGGAATTGA+GGG | 0.494037 | 5.4:-17894165 | None:intergenic |
| TGAAGCAGTGGTGACAATAA+GGG | 0.495914 | 5.4:-17894061 | None:intergenic |
| GGTTGAAGTTTGAGCAGCCA+TGG | 0.516512 | 5.4:-17893898 | None:intergenic |
| GTAGCAGAATAGAGAAGAAG+AGG | 0.521798 | 5.4:-17894014 | None:intergenic |
| GTTGTATCCCTAATGCATGC+AGG | 0.526565 | 5.4:+17894570 | MS.gene070100:CDS |
| TAAGCCTTAATGGATCTTTG+AGG | 0.528933 | 5.4:-17894512 | None:intergenic |
| CATGGAAATGTTTGTGAAGG+TGG | 0.530274 | 5.4:-17894092 | None:intergenic |
| GAAGAAGAGGGGATTCAGTG+AGG | 0.537045 | 5.4:-17894001 | None:intergenic |
| TTGATTAAGGATGTTGCTGT+TGG | 0.542851 | 5.4:+17894653 | MS.gene070100:CDS |
| AGCAGAATAGAGAAGAAGAG+GGG | 0.546586 | 5.4:-17894012 | None:intergenic |
| GTAAAGTTTGAAGCTTTGAA+GGG | 0.548356 | 5.4:-17894307 | None:intergenic |
| AAAAGGTAGTTTGAGCAAGA+AGG | 0.551661 | 5.4:-17894116 | None:intergenic |
| AAATGTTTGTGAAGGTGGTA+AGG | 0.557933 | 5.4:-17894087 | None:intergenic |
| TTTCTGATAATGGTGAAGTG+GGG | 0.567781 | 5.4:-17894220 | None:intergenic |
| GAAGCAGTGGTGACAATAAG+GGG | 0.577265 | 5.4:-17894060 | None:intergenic |
| GTTGAAGTTTGAGCAGCCAT+GGG | 0.577914 | 5.4:-17893897 | None:intergenic |
| CACATACCTAACAAAGGTAG+AGG | 0.580606 | 5.4:+17893939 | MS.gene070100:CDS |
| GAGGGTGTATGAGAGAAAAG+AGG | 0.582689 | 5.4:-17894038 | None:intergenic |
| TAGTTTGAGCAAGAAGGACA+TGG | 0.592796 | 5.4:-17894110 | None:intergenic |
| TCAGTATTATGTTCATCACC+AGG | 0.593180 | 5.4:-17894617 | None:intergenic |
| TATGTGGAAAGTGGTGAACC+TGG | 0.596809 | 5.4:+17894599 | MS.gene070100:CDS |
| AGGTTTGATTATGTGGAAAG+TGG | 0.601381 | 5.4:+17894590 | MS.gene070100:CDS |
| ACATGACCAAGATGAGTGAG+AGG | 0.606296 | 5.4:-17894174 | None:intergenic |
| TAAAGTTTGAAGCTTTGAAG+GGG | 0.608315 | 5.4:-17894306 | None:intergenic |
| GTGGTAAGGTTCTGAAGCAG+TGG | 0.614912 | 5.4:-17894073 | None:intergenic |
| AGCTAATTGGAATGATGGAG+AGG | 0.615772 | 5.4:+17894787 | MS.gene070100:CDS |
| AGGAATTGAGGGCATTGGAG+AGG | 0.625591 | 5.4:-17894154 | None:intergenic |
| GCTTGAACTTGAAGTTCACA+AGG | 0.648973 | 5.4:-17894245 | None:intergenic |
| CAGTGGTGACAATAAGGGGA+GGG | 0.657839 | 5.4:-17894056 | None:intergenic |
| GTGCTCCAATCTCAAGAGAG+AGG | 0.658701 | 5.4:-17893919 | None:intergenic |
| GGACATGGAAATGTTTGTGA+AGG | 0.669839 | 5.4:-17894095 | None:intergenic |
| GCAGTGGTGACAATAAGGGG+AGG | 0.670959 | 5.4:-17894057 | None:intergenic |
| GCAACACAAGATCTCAACGC+TGG | 0.676546 | 5.4:+17893969 | MS.gene070100:CDS |
| TTAGATAAGAAAATCACATG+TGG | 0.682821 | 5.4:-17894824 | None:intergenic |
| ATGCATTAGGGATACAACAA+TGG | 0.688941 | 5.4:-17894565 | None:intergenic |
| TTGGAGCACATACCTAACAA+AGG | 0.691591 | 5.4:+17893933 | MS.gene070100:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATAATACTGATATTGTTTTT+AGG | + | chr5.4:17894630-17894649 | MS.gene070100:CDS | 15.0% |
| !!! | AAGCTATTTTAGGATTAATA+AGG | + | chr5.4:17894691-17894710 | MS.gene070100:intron | 20.0% |
| !!! | AGATTTGTATAAGCTATTTT+AGG | + | chr5.4:17894681-17894700 | MS.gene070100:intron | 20.0% |
| !!! | ATTAAACTCTTTTTGCTTAA+TGG | + | chr5.4:17894469-17894488 | MS.gene070100:CDS | 20.0% |
| !!! | TATTGTTTTTAGGTTGATTA+AGG | + | chr5.4:17894640-17894659 | MS.gene070100:CDS | 20.0% |
| ! | AAACTTAGCATTATCAACTA+TGG | - | chr5.4:17894366-17894385 | None:intergenic | 25.0% |
| ! | TTAATAAGGATTACTCAACA+AGG | + | chr5.4:17894705-17894724 | MS.gene070100:intron | 25.0% |
| AACAAGGAAAAAGATCTTGA+TGG | + | chr5.4:17894721-17894740 | MS.gene070100:intron | 30.0% | |
| ACTATGGAATCATGATCATT+AGG | - | chr5.4:17894350-17894369 | None:intergenic | 30.0% | |
| ATTGAAGCTAATTGGAATGA+TGG | + | chr5.4:17894782-17894801 | MS.gene070100:CDS | 30.0% | |
| CAAACTTTACTTTCACTTCA+TGG | + | chr5.4:17894320-17894339 | MS.gene070100:CDS | 30.0% | |
| GTAAATTCCATAAGCCTTAA+TGG | - | chr5.4:17894525-17894544 | None:intergenic | 30.0% | |
| TTCAGCTGAAAAATTCATGT+GGG | - | chr5.4:17894420-17894439 | None:intergenic | 30.0% | |
| ! | AAGATCTTGATGGATGATTA+TGG | + | chr5.4:17894731-17894750 | MS.gene070100:intron | 30.0% |
| ! | AGTAAAGTTTGAAGCTTTGA+AGG | - | chr5.4:17894311-17894330 | None:intergenic | 30.0% |
| ! | GGTTAAAAAAAGTAGCGTTT+TGG | - | chr5.4:17894547-17894566 | None:intergenic | 30.0% |
| ! | TCACAAGGTTTTTCTGATAA+TGG | - | chr5.4:17894233-17894252 | None:intergenic | 30.0% |
| !! | GTAAAGTTTGAAGCTTTGAA+GGG | - | chr5.4:17894310-17894329 | None:intergenic | 30.0% |
| !! | TAAAGTTTGAAGCTTTGAAG+GGG | - | chr5.4:17894309-17894328 | None:intergenic | 30.0% |
| !!! | TTTTCTGATAATGGTGAAGT+GGG | - | chr5.4:17894224-17894243 | None:intergenic | 30.0% |
| !!! | TTTTTCTGATAATGGTGAAG+TGG | - | chr5.4:17894225-17894244 | None:intergenic | 30.0% |
| AAAAGGTAGTTTGAGCAAGA+AGG | - | chr5.4:17894119-17894138 | None:intergenic | 35.0% | |
| AAATGTTTGTGAAGGTGGTA+AGG | - | chr5.4:17894090-17894109 | None:intergenic | 35.0% | |
| AATCAAACCTGCATGCATTA+GGG | - | chr5.4:17894580-17894599 | None:intergenic | 35.0% | |
| AATGGAACCTTATTCCCAAA+AGG | + | chr5.4:17894487-17894506 | MS.gene070100:CDS | 35.0% | |
| AGGTTTGATTATGTGGAAAG+TGG | + | chr5.4:17894590-17894609 | MS.gene070100:CDS | 35.0% | |
| ATGCATTAGGGATACAACAA+TGG | - | chr5.4:17894568-17894587 | None:intergenic | 35.0% | |
| ATGGAACCTTATTCCCAAAA+GGG | + | chr5.4:17894488-17894507 | MS.gene070100:CDS | 35.0% | |
| CAAAGATCCATTAAGGCTTA+TGG | + | chr5.4:17894515-17894534 | MS.gene070100:CDS | 35.0% | |
| GTTCAGCTGAAAAATTCATG+TGG | - | chr5.4:17894421-17894440 | None:intergenic | 35.0% | |
| TAATCAAACCTGCATGCATT+AGG | - | chr5.4:17894581-17894600 | None:intergenic | 35.0% | |
| TAGCAGAATAGAGAAGAAGA+GGG | - | chr5.4:17894016-17894035 | None:intergenic | 35.0% | |
| TCAGCTGAAAAATTCATGTG+GGG | - | chr5.4:17894419-17894438 | None:intergenic | 35.0% | |
| TCAGTATTATGTTCATCACC+AGG | - | chr5.4:17894620-17894639 | None:intergenic | 35.0% | |
| TGAGATCTTGTGTTGCAATA+AGG | - | chr5.4:17893965-17893984 | None:intergenic | 35.0% | |
| TTGATTAAGGATGTTGCTGT+TGG | + | chr5.4:17894653-17894672 | MS.gene070100:CDS | 35.0% | |
| ! | GTTTTGCTTGTGAATGTGAT+AGG | + | chr5.4:17894753-17894772 | MS.gene070100:intron | 35.0% |
| ! | TAAGCCTTAATGGATCTTTG+AGG | - | chr5.4:17894515-17894534 | None:intergenic | 35.0% |
| !! | TTTCTGATAATGGTGAAGTG+GGG | - | chr5.4:17894223-17894242 | None:intergenic | 35.0% |
| AAGATGAGTGAGAGGAATTG+AGG | - | chr5.4:17894169-17894188 | None:intergenic | 40.0% | |
| AGATGAGTGAGAGGAATTGA+GGG | - | chr5.4:17894168-17894187 | None:intergenic | 40.0% | |
| AGCAGAATAGAGAAGAAGAG+GGG | - | chr5.4:17894015-17894034 | None:intergenic | 40.0% | |
| ATAAGGCCTCTACCTTTGTT+AGG | - | chr5.4:17893948-17893967 | None:intergenic | 40.0% | |
| CACATACCTAACAAAGGTAG+AGG | + | chr5.4:17893939-17893958 | MS.gene070100:CDS | 40.0% | |
| CATGGAAATGTTTGTGAAGG+TGG | - | chr5.4:17894095-17894114 | None:intergenic | 40.0% | |
| GCTTGAACTTGAAGTTCACA+AGG | - | chr5.4:17894248-17894267 | None:intergenic | 40.0% | |
| GGACATGGAAATGTTTGTGA+AGG | - | chr5.4:17894098-17894117 | None:intergenic | 40.0% | |
| GGGATTCAGTGAGGATTATT+TGG | - | chr5.4:17893995-17894014 | None:intergenic | 40.0% | |
| GGTGCAAGATTGAAGCTAAT+TGG | + | chr5.4:17894774-17894793 | MS.gene070100:intron | 40.0% | |
| GTAGCAGAATAGAGAAGAAG+AGG | - | chr5.4:17894017-17894036 | None:intergenic | 40.0% | |
| TAGTTTGAGCAAGAAGGACA+TGG | - | chr5.4:17894113-17894132 | None:intergenic | 40.0% | |
| TCAATTCCTCTCACTCATCT+TGG | + | chr5.4:17894168-17894187 | MS.gene070100:CDS | 40.0% | |
| TGAAGCAGTGGTGACAATAA+GGG | - | chr5.4:17894064-17894083 | None:intergenic | 40.0% | |
| TGCATGCAGGTTTGATTATG+TGG | + | chr5.4:17894583-17894602 | MS.gene070100:CDS | 40.0% | |
| TTCAACCTCTCTCTTGAGAT+TGG | + | chr5.4:17893914-17893933 | MS.gene070100:CDS | 40.0% | |
| TTGGAGCACATACCTAACAA+AGG | + | chr5.4:17893933-17893952 | MS.gene070100:CDS | 40.0% | |
| ! | AGCTAATTGGAATGATGGAG+AGG | + | chr5.4:17894787-17894806 | MS.gene070100:CDS | 40.0% |
| ACATGACCAAGATGAGTGAG+AGG | - | chr5.4:17894177-17894196 | None:intergenic | 45.0% | |
| CTGAAGCAGTGGTGACAATA+AGG | - | chr5.4:17894065-17894084 | None:intergenic | 45.0% | |
| GAAGCAGTGGTGACAATAAG+GGG | - | chr5.4:17894063-17894082 | None:intergenic | 45.0% | |
| GCTGAAAAATTCATGTGGGG+TGG | - | chr5.4:17894416-17894435 | None:intergenic | 45.0% | |
| GGGTCCTCAAAGATCCATTA+AGG | + | chr5.4:17894508-17894527 | MS.gene070100:CDS | 45.0% | |
| GTTGTATCCCTAATGCATGC+AGG | + | chr5.4:17894570-17894589 | MS.gene070100:CDS | 45.0% | |
| TATGTGGAAAGTGGTGAACC+TGG | + | chr5.4:17894599-17894618 | MS.gene070100:CDS | 45.0% | |
| TTCATGTGGGGTGGACAAAA+AGG | - | chr5.4:17894407-17894426 | None:intergenic | 45.0% | |
| ! | GAGGGTGTATGAGAGAAAAG+AGG | - | chr5.4:17894041-17894060 | None:intergenic | 45.0% |
| !! | GGATCTTTGAGGACCCTTTT+GGG | - | chr5.4:17894504-17894523 | None:intergenic | 45.0% |
| !! | TGAGGACCCTTTTGGGAATA+AGG | - | chr5.4:17894497-17894516 | None:intergenic | 45.0% |
| !! | TGGATCTTTGAGGACCCTTT+TGG | - | chr5.4:17894505-17894524 | None:intergenic | 45.0% |
| !!! | GGCATTTTTGGCTGCAGAAA+AGG | - | chr5.4:17894136-17894155 | None:intergenic | 45.0% |
| CAGTGGTGACAATAAGGGGA+GGG | - | chr5.4:17894059-17894078 | None:intergenic | 50.0% | |
| GAAGAAGAGGGGATTCAGTG+AGG | - | chr5.4:17894004-17894023 | None:intergenic | 50.0% | |
| GCAACACAAGATCTCAACGC+TGG | + | chr5.4:17893969-17893988 | MS.gene070100:CDS | 50.0% | |
| GTGCTCCAATCTCAAGAGAG+AGG | - | chr5.4:17893922-17893941 | None:intergenic | 50.0% | |
| ! | GTGAGAGGAATTGAGGGCAT+TGG | - | chr5.4:17894162-17894181 | None:intergenic | 50.0% |
| !! | AGGAATTGAGGGCATTGGAG+AGG | - | chr5.4:17894157-17894176 | None:intergenic | 50.0% |
| !! | GTGGTAAGGTTCTGAAGCAG+TGG | - | chr5.4:17894076-17894095 | None:intergenic | 50.0% |
| !!! | GGGCATTGGAGAGGCATTTT+TGG | - | chr5.4:17894148-17894167 | None:intergenic | 50.0% |
| GCAGTGGTGACAATAAGGGG+AGG | - | chr5.4:17894060-17894079 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.4 | gene | 17893900 | 17894833 | 17893900 | ID=MS.gene070100 |
| chr5.4 | mRNA | 17893900 | 17894833 | 17893900 | ID=MS.gene070100.t1;Parent=MS.gene070100 |
| chr5.4 | exon | 17893900 | 17894677 | 17893900 | ID=MS.gene070100.t1.exon1;Parent=MS.gene070100.t1 |
| chr5.4 | CDS | 17893900 | 17894677 | 17893900 | ID=cds.MS.gene070100.t1;Parent=MS.gene070100.t1 |
| chr5.4 | exon | 17894775 | 17894833 | 17894775 | ID=MS.gene070100.t1.exon2;Parent=MS.gene070100.t1 |
| chr5.4 | CDS | 17894775 | 17894833 | 17894775 | ID=cds.MS.gene070100.t1;Parent=MS.gene070100.t1 |
| Gene Sequence |
| Protein sequence |
