AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070105

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070105.t1 MTR_0856s0010 60.625 160 61 1 1 158 1 160 5.33e-58 194
MS.gene070105.t1 MTR_6g051425 31.618 272 174 5 35 296 20 289 1.43e-37 144
MS.gene070105.t1 MTR_7g115320 67.470 83 24 1 119 201 6 85 5.72e-28 108
MS.gene070105.t1 MTR_4g035820 29.779 272 153 11 25 273 68 324 2.95e-21 99.4
MS.gene070105.t1 MTR_0558s0020 26.255 259 164 6 36 269 40 296 4.52e-21 94.7
MS.gene070105.t1 MTR_1g008290 33.333 129 83 1 97 225 19 144 9.01e-20 87.0
MS.gene070105.t1 MTR_8g446020 28.729 181 123 3 93 269 78 256 3.51e-18 85.5
MS.gene070105.t1 MTR_1g070355 27.966 236 154 9 38 263 80 309 1.41e-17 87.4
MS.gene070105.t1 MTR_7g446060 43.662 71 40 0 126 196 18 88 1.46e-16 77.4
MS.gene070105.t1 MTR_7g446510 30.168 179 123 2 105 281 155 333 1.81e-16 81.6
MS.gene070105.t1 MTR_7g446480 30.726 179 122 2 105 281 140 318 1.81e-16 81.6
MS.gene070105.t1 MTR_6g022070 29.605 152 91 2 323 468 1 142 1.67e-15 79.0
MS.gene070105.t1 MTR_5g076070 25.862 232 158 6 35 257 56 282 6.19e-14 73.6
MS.gene070105.t1 MTR_1g083640 24.348 230 147 6 36 240 55 282 4.37e-13 70.9
MS.gene070105.t1 MTR_5g023310 28.846 156 44 2 164 319 16 104 7.26e-13 65.9
MS.gene070105.t1 MTR_6g090505 25.843 178 130 2 93 268 53 230 9.30e-13 70.9
MS.gene070105.t1 MTR_1g013060 26.887 212 137 6 31 229 23 229 1.83e-12 68.2
MS.gene070105.t1 MTR_1g019850 31.788 151 92 5 14 160 53 196 6.92e-12 66.6
MS.gene070105.t1 MTR_7g446520 27.815 151 108 1 122 271 165 315 7.12e-12 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070105.t1 AT4G15090 33.713 703 439 12 3 697 25 708 1.20e-129 405
MS.gene070105.t1 AT4G15090 33.713 703 439 12 3 697 25 708 1.20e-129 405
MS.gene070105.t1 AT4G15090 33.713 703 439 12 3 697 25 708 1.20e-129 405
MS.gene070105.t1 AT4G15090 33.713 703 439 12 3 697 25 708 1.20e-129 405
MS.gene070105.t1 AT4G15090 33.713 703 439 12 3 697 25 708 1.20e-129 405
MS.gene070105.t1 AT4G38180 34.750 659 382 15 17 637 47 695 6.19e-121 381
MS.gene070105.t1 AT1G52520 33.441 619 392 11 32 643 85 690 1.49e-113 359
MS.gene070105.t1 AT1G76320 30.945 656 414 11 36 685 1 623 5.78e-106 338
MS.gene070105.t1 AT1G76320 30.687 655 417 10 36 685 1 623 2.41e-105 338
MS.gene070105.t1 AT1G76320 30.687 655 417 10 36 685 1 623 3.69e-105 338
MS.gene070105.t1 AT1G76320 30.687 655 417 10 36 685 1 623 3.69e-105 338
MS.gene070105.t1 AT3G22170 31.994 622 370 8 26 628 64 651 4.94e-105 340
MS.gene070105.t1 AT3G22170 31.994 622 370 8 26 628 64 651 4.94e-105 340
MS.gene070105.t1 AT2G27110 32.097 620 376 13 18 628 32 615 9.58e-97 318
MS.gene070105.t1 AT2G27110 32.097 620 376 13 18 628 32 615 9.58e-97 318
MS.gene070105.t1 AT1G80010 30.366 629 410 11 32 643 95 712 1.74e-93 307
MS.gene070105.t1 AT2G32250 30.887 654 390 10 24 670 26 624 5.23e-92 304
MS.gene070105.t1 AT2G32250 30.887 654 390 10 24 670 26 624 2.19e-91 303
MS.gene070105.t1 AT2G32250 30.887 654 390 10 24 670 26 624 2.19e-91 303
MS.gene070105.t1 AT2G32250 30.887 654 390 10 24 670 26 624 2.81e-91 303
MS.gene070105.t1 AT2G32250 30.887 654 390 10 24 670 26 624 2.81e-91 303
MS.gene070105.t1 AT2G32250 30.887 654 390 10 24 670 26 624 2.81e-91 303
MS.gene070105.t1 AT2G32250 30.721 638 384 9 36 670 1 583 6.10e-90 298
MS.gene070105.t1 AT2G32250 30.721 638 384 9 36 670 1 583 6.16e-90 298
MS.gene070105.t1 AT3G06250 32.566 565 328 13 29 589 187 702 6.79e-89 295
MS.gene070105.t1 AT3G06250 32.566 565 328 13 29 589 187 702 6.79e-89 295
MS.gene070105.t1 AT3G06250 32.566 565 328 13 29 589 187 702 6.79e-89 295
MS.gene070105.t1 AT2G27110 35.388 438 268 6 194 628 45 470 4.10e-86 286
MS.gene070105.t1 AT4G19990 30.757 621 371 11 15 617 4 583 7.29e-85 284
MS.gene070105.t1 AT4G19990 30.757 621 371 11 15 617 18 597 9.17e-85 285
MS.gene070105.t1 AT4G19990 30.757 621 371 11 15 617 4 583 9.93e-85 284
MS.gene070105.t1 AT4G19990 30.757 621 371 11 15 617 4 583 9.93e-85 284
MS.gene070105.t1 AT4G19990 30.757 621 371 11 15 617 4 583 1.74e-84 284
MS.gene070105.t1 AT4G19990 30.757 621 371 11 15 617 4 583 1.74e-84 284
MS.gene070105.t1 AT5G18960 30.998 571 328 12 31 589 210 726 1.78e-82 279
MS.gene070105.t1 AT5G18960 30.998 571 328 12 31 589 210 726 1.78e-82 279
MS.gene070105.t1 AT4G38170 36.082 388 237 5 206 591 21 399 5.35e-82 271
MS.gene070105.t1 AT4G19990 35.800 419 261 4 201 617 114 526 1.13e-78 266
MS.gene070105.t1 AT1G10240 27.740 584 368 16 32 588 48 604 1.31e-60 216
MS.gene070105.t1 AT1G10240 27.740 584 368 16 32 588 48 604 1.31e-60 216
MS.gene070105.t1 AT5G28530 25.473 581 388 13 32 588 55 614 6.88e-54 197
MS.gene070105.t1 AT5G28530 25.473 581 388 13 32 588 55 614 6.88e-54 197
MS.gene070105.t1 AT5G28530 25.473 581 392 13 32 588 55 618 2.79e-53 196
MS.gene070105.t1 AT5G28530 24.214 318 219 5 275 588 252 551 1.21e-25 112
MS.gene070105.t1 AT5G28530 24.214 318 223 5 275 588 252 555 5.35e-25 110

Find 110 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAGCTGCATATGAATATTT+TGG 0.129890 5.4:+17946225 MS.gene070105:CDS
CACGATCAAAAGAAACTTTA+AGG 0.142944 5.4:-17947106 None:intergenic
TAAAGAATATGCTTCTAAAA+AGG 0.156428 5.4:+17945642 MS.gene070105:CDS
TCCCTTCGAATAATTCTATT+TGG 0.180280 5.4:-17947494 None:intergenic
GTTACCTTCCACGATATTTA+AGG 0.186766 5.4:-17947024 None:intergenic
TCGCATGAGTATATTCTTCT+TGG 0.219492 5.4:-17946950 None:intergenic
CAAATCTAGCTTGCTATGTT+TGG 0.233030 5.4:+17947430 MS.gene070105:CDS
CGCCCGCTTCATCATTGATT+TGG 0.236954 5.4:-17945954 None:intergenic
AGCATACATGCTTTCTTTGA+TGG 0.264138 5.4:+17946777 MS.gene070105:CDS
TTCATGTACGAAATGTGTTT+TGG 0.269388 5.4:+17946187 MS.gene070105:CDS
ATATACTCATGCGAAGTTTA+AGG 0.285045 5.4:+17946959 MS.gene070105:CDS
AAAAGGCATATCATATTTAT+TGG 0.296866 5.4:-17946280 None:intergenic
TGAAGACACTTCCGACTAAA+AGG 0.299870 5.4:+17945776 MS.gene070105:CDS
TGCTCATTGTTTGAGTTTAG+AGG 0.314378 5.4:+17947152 MS.gene070105:CDS
ACATAATTGGCACCCATCTA+TGG 0.318132 5.4:-17946707 None:intergenic
CATGGTCAATCAACTTTACT+TGG 0.319017 5.4:+17946327 MS.gene070105:CDS
CCTGAAAAGCTTAGTAGATA+TGG 0.320188 5.4:+17946540 MS.gene070105:CDS
GTGTTGGAGGAGATGTTAAT+TGG 0.326688 5.4:+17947062 MS.gene070105:CDS
TTTCTTCTATGATATAGATT+TGG 0.334701 5.4:+17946155 MS.gene070105:CDS
TGAATTCATGAAGTGTTTCA+TGG 0.341741 5.4:-17947409 None:intergenic
AACTTAGCAGTAATCTTTGC+AGG 0.345757 5.4:-17945808 None:intergenic
AAATCTAGCTTGCTATGTTT+GGG 0.356420 5.4:+17947431 MS.gene070105:CDS
TCACAAACATCAAAATTGTT+TGG 0.358937 5.4:-17945508 None:intergenic
AGTTGGGAAAAGATGAATTA+TGG 0.360559 5.4:+17945827 MS.gene070105:CDS
AACTTTACTTGGATGTGGTC+TGG 0.388630 5.4:+17946338 MS.gene070105:CDS
CTTGAATAACCTTGACTTAG+TGG 0.398152 5.4:-17945893 None:intergenic
GCAAGTTGCGAGCCTTCATC+AGG 0.400046 5.4:+17945580 MS.gene070105:CDS
TGTACAATGAGCGCCATAGA+TGG 0.404513 5.4:+17946694 MS.gene070105:CDS
AATGGAGTTGATAACTTTAG+TGG 0.409161 5.4:+17945550 MS.gene070105:CDS
CTGTCAAACCTATCCATTTG+TGG 0.418494 5.4:-17947320 None:intergenic
TGCAAAGATTACTGCTAAGT+TGG 0.422853 5.4:+17945810 MS.gene070105:CDS
CTAATTGTGTGAGAGTGGTT+AGG 0.441187 5.4:-17945868 None:intergenic
GGAATTATGTTAATAGAGAA+AGG 0.442025 5.4:+17946058 MS.gene070105:CDS
ATTTACAAATTGATGCAAAC+TGG 0.443341 5.4:-17946817 None:intergenic
CACCACCTATGTCTAGTAGT+AGG 0.443756 5.4:-17946492 None:intergenic
CACGTGCCTTAGTTGTCAAT+TGG 0.446141 5.4:-17947649 None:intergenic
AATAGAGAAAGGCGTATCAT+TGG 0.465390 5.4:+17946069 MS.gene070105:CDS
CTAGACATAGGTGGTGTTTG+TGG 0.469131 5.4:+17946499 MS.gene070105:CDS
CCATTGCGAGTCACGCGTAA+AGG 0.473557 5.4:+17947545 MS.gene070105:CDS
TTGAATAACCTTGACTTAGT+GGG 0.483800 5.4:-17945892 None:intergenic
CACCTCCGCTACAGATGAAA+AGG 0.484298 5.4:-17945608 None:intergenic
CACTAAGTCAAGGTTATTCA+AGG 0.496285 5.4:+17945894 MS.gene070105:CDS
CAATCAACTTTACTTGGATG+TGG 0.499442 5.4:+17946333 MS.gene070105:CDS
GCAAAGATTACTGCTAAGTT+GGG 0.503061 5.4:+17945811 MS.gene070105:CDS
ACACCAACAAATGCAGCAAA+AGG 0.504617 5.4:-17946297 None:intergenic
AGTAGGGAATACTGATTGAA+TGG 0.504923 5.4:-17946475 None:intergenic
GCGTGACTCGCAATGGACTA+CGG 0.506171 5.4:-17947538 None:intergenic
GACAGAGTTGAAGCCACAAA+TGG 0.507415 5.4:+17947307 MS.gene070105:CDS
AGAGAAGGAAATCATGTGTC+GGG 0.510956 5.4:+17945739 MS.gene070105:CDS
TATTATCACTGACCAATGTA+AGG 0.514760 5.4:+17946440 MS.gene070105:CDS
GAAAGGCGTATCATTGGAAA+AGG 0.517095 5.4:+17946075 MS.gene070105:CDS
ACATCAAAGAAAGGAGAGGA+TGG 0.519853 5.4:+17945685 MS.gene070105:CDS
TGCTATTTACCACGTGTTGG+AGG 0.524536 5.4:+17947049 MS.gene070105:CDS
TTTCTATGAGGTTGCTGAAG+TGG 0.529465 5.4:+17947355 MS.gene070105:CDS
TTGAGCTGGAACATAATGAT+TGG 0.533395 5.4:+17946661 MS.gene070105:CDS
GAAAAGGACATCCCTGATGA+AGG 0.537910 5.4:-17945592 None:intergenic
TTGTGCAGAGAAGCAGGAAG+CGG 0.537951 5.4:+17947609 MS.gene070105:CDS
ATTTGGCCTCGAGTCAGTGT+TGG 0.539381 5.4:-17947477 None:intergenic
AAGATTAGAACATCAAAGAA+AGG 0.539533 5.4:+17945676 MS.gene070105:CDS
CCTTGCTATTTACCACGTGT+TGG 0.542509 5.4:+17947046 MS.gene070105:CDS
TCATGGCTTCGTTGTATGCA+AGG 0.543000 5.4:+17946402 MS.gene070105:CDS
CAGACTATTGTTAGAGACGC+TGG 0.544566 5.4:+17945997 MS.gene070105:CDS
ATCCAAATCAATGATGAAGC+GGG 0.553848 5.4:+17945952 MS.gene070105:CDS
GGGTGACCAATTGACAACTA+AGG 0.555202 5.4:+17947643 MS.gene070105:CDS
GGCCAAATAGAATTATTCGA+AGG 0.555904 5.4:+17947492 MS.gene070105:CDS
AACATAATGATTGGCTGAGT+GGG 0.556371 5.4:+17946670 MS.gene070105:CDS
CCTTTACGCGTGACTCGCAA+TGG 0.556393 5.4:-17947545 None:intergenic
TGGACACAACAATTCCTTGT+GGG 0.558651 5.4:+17946901 MS.gene070105:CDS
GTACAATGAGCGCCATAGAT+GGG 0.559433 5.4:+17946695 MS.gene070105:CDS
ATGCAAGGGAAGGCTCCTGC+AGG 0.564052 5.4:+17946417 MS.gene070105:CDS
TGTTACCTCCTTAAATATCG+TGG 0.565341 5.4:+17947016 MS.gene070105:CDS
GCCAAATAGAATTATTCGAA+GGG 0.566470 5.4:+17947493 MS.gene070105:CDS
ATCATTGGAAAAGGAGGAGA+CGG 0.566514 5.4:+17946084 MS.gene070105:CDS
ACCACCTATGTCTAGTAGTA+GGG 0.566962 5.4:-17946491 None:intergenic
AGTTGAAGCCACAAATGGAT+AGG 0.568044 5.4:+17947312 MS.gene070105:CDS
AGCATTCACAACTAACATCA+TGG 0.572632 5.4:-17947133 None:intergenic
CATCAAAGAAAGGAGAGGAT+GGG 0.575239 5.4:+17945686 MS.gene070105:CDS
ACCTCCTTAAATATCGTGGA+AGG 0.586701 5.4:+17947020 MS.gene070105:CDS
ATGCTATCTTGCTCAAGAGA+AGG 0.587758 5.4:+17945724 MS.gene070105:CDS
AAGAGAAGGAAATCATGTGT+CGG 0.591467 5.4:+17945738 MS.gene070105:CDS
GGAAGTGTCTTCAATGTACA+TGG 0.592537 5.4:-17945766 None:intergenic
CTTGTGCAAGCATTTCTATG+AGG 0.596711 5.4:+17947343 MS.gene070105:CDS
TGGTCAGTGATAATACCTGC+AGG 0.597873 5.4:-17946432 None:intergenic
TGGGGCTAATTGTGTGAGAG+TGG 0.603064 5.4:-17945873 None:intergenic
TGAATAACCTTGACTTAGTG+GGG 0.603118 5.4:-17945891 None:intergenic
TCAATGATGAAGCGGGCGTG+AGG 0.603491 5.4:+17945959 MS.gene070105:CDS
AATCCAAATCAATGATGAAG+CGG 0.605155 5.4:+17945951 MS.gene070105:CDS
ACCTTCCACGATATTTAAGG+AGG 0.607235 5.4:-17947021 None:intergenic
CAAGTAAAGTTGATTGACCA+TGG 0.609756 5.4:-17946326 None:intergenic
TATTCCCTACTACTAGACAT+AGG 0.615682 5.4:+17946487 MS.gene070105:CDS
CATGGCTTCGTTGTATGCAA+GGG 0.615709 5.4:+17946403 MS.gene070105:CDS
CTTACTAAAACATACTTCAG+TGG 0.627472 5.4:-17947230 None:intergenic
ATCGATGAGCTTGACCCACA+AGG 0.628232 5.4:-17946915 None:intergenic
TCTGGTTTCAGCTGAAGACA+CGG 0.628431 5.4:+17946356 MS.gene070105:CDS
CCAACACGTGGTAAATAGCA+AGG 0.628598 5.4:-17947046 None:intergenic
GCTTCGTTGTATGCAAGGGA+AGG 0.629895 5.4:+17946407 MS.gene070105:CDS
AGGCGTATCATTGGAAAAGG+AGG 0.630655 5.4:+17946078 MS.gene070105:CDS
TACTCATGCGAAGTTTAAGG+AGG 0.641837 5.4:+17946962 MS.gene070105:CDS
GAAGTTTAAGGAGGTTCAAG+CGG 0.643158 5.4:+17946971 MS.gene070105:CDS
TAGAACATCAAAGAAAGGAG+AGG 0.643941 5.4:+17945681 MS.gene070105:CDS
ATGGACACAACAATTCCTTG+TGG 0.653497 5.4:+17946900 MS.gene070105:CDS
GAACATAATGATTGGCTGAG+TGG 0.655057 5.4:+17946669 MS.gene070105:CDS
CAATTAGCCCCACTAAGTCA+AGG 0.658920 5.4:+17945884 MS.gene070105:CDS
CAAGTTGCGAGCCTTCATCA+GGG 0.660640 5.4:+17945581 MS.gene070105:CDS
GAAGTTCCAACACTGACTCG+AGG 0.694071 5.4:+17947471 MS.gene070105:CDS
TTTGTTGGTGTGAATCACCA+TGG 0.705691 5.4:+17946309 MS.gene070105:CDS
ACTATTGTTAGAGACGCTGG+AGG 0.710750 5.4:+17946000 MS.gene070105:CDS
TTAACATCTCCTCCAACACG+TGG 0.712095 5.4:-17947058 None:intergenic
TCCCTACTACTAGACATAGG+TGG 0.718251 5.4:+17946490 MS.gene070105:CDS
CTGGTATGTCGACAACACAA+CGG 0.726317 5.4:+17946748 MS.gene070105:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAAGATTTTTTCAACATAAT+TGG - chr5.4:17946723-17946742 None:intergenic 15.0%
!!! TATGTTGAAAAAATCTTTTT+GGG + chr5.4:17946725-17946744 MS.gene070105:CDS 15.0%
!!! TTATGTTGAAAAAATCTTTT+TGG + chr5.4:17946724-17946743 MS.gene070105:CDS 15.0%
!! AGAAAAAGAAGATAAAACTA+TGG + chr5.4:17947382-17947401 MS.gene070105:CDS 20.0%
!! TTTCTTCTATGATATAGATT+TGG + chr5.4:17946155-17946174 MS.gene070105:CDS 20.0%
!!! AAAAGGCATATCATATTTAT+TGG - chr5.4:17946283-17946302 None:intergenic 20.0%
!!! AAAGAATATGCTTCTAAAAA+GGG + chr5.4:17945643-17945662 MS.gene070105:CDS 20.0%
!!! TAAAGAATATGCTTCTAAAA+AGG + chr5.4:17945642-17945661 MS.gene070105:CDS 20.0%
! AAGATTAGAACATCAAAGAA+AGG + chr5.4:17945676-17945695 MS.gene070105:CDS 25.0%
! ATTTACAAATTGATGCAAAC+TGG - chr5.4:17946820-17946839 None:intergenic 25.0%
! GATGGAGCAAAAATATTAAA+AGG + chr5.4:17947252-17947271 MS.gene070105:CDS 25.0%
! GGAATTATGTTAATAGAGAA+AGG + chr5.4:17946058-17946077 MS.gene070105:CDS 25.0%
! TCACAAACATCAAAATTGTT+TGG - chr5.4:17945511-17945530 None:intergenic 25.0%
!! TTTTGTTAACAAATTTGAGC+TGG + chr5.4:17946647-17946666 MS.gene070105:CDS 25.0%
!!! AACAATTTTGATGTTTGTGA+TGG + chr5.4:17945511-17945530 MS.gene070105:CDS 25.0%
!!! TGAAGTATGTTTTAGTAAGA+TGG + chr5.4:17947234-17947253 MS.gene070105:CDS 25.0%
AATCCAAATCAATGATGAAG+CGG + chr5.4:17945951-17945970 MS.gene070105:CDS 30.0%
AGTTGGGAAAAGATGAATTA+TGG + chr5.4:17945827-17945846 MS.gene070105:CDS 30.0%
ATATACTCATGCGAAGTTTA+AGG + chr5.4:17946959-17946978 MS.gene070105:CDS 30.0%
CACGATCAAAAGAAACTTTA+AGG - chr5.4:17947109-17947128 None:intergenic 30.0%
CATCTGAATTCGAAGAAAAA+TGG + chr5.4:17946619-17946638 MS.gene070105:CDS 30.0%
CTTACTAAAACATACTTCAG+TGG - chr5.4:17947233-17947252 None:intergenic 30.0%
GCCAAATAGAATTATTCGAA+GGG + chr5.4:17947493-17947512 MS.gene070105:CDS 30.0%
TATTATCACTGACCAATGTA+AGG + chr5.4:17946440-17946459 MS.gene070105:CDS 30.0%
TCCCTTCGAATAATTCTATT+TGG - chr5.4:17947497-17947516 None:intergenic 30.0%
TTGAATAACCTTGACTTAGT+GGG - chr5.4:17945895-17945914 None:intergenic 30.0%
! AGAGCTGCATATGAATATTT+TGG + chr5.4:17946225-17946244 MS.gene070105:CDS 30.0%
! AGCTGCATATGAATATTTTG+GGG + chr5.4:17946227-17946246 MS.gene070105:CDS 30.0%
! CATATCTACTAAGCTTTTCA+GGG - chr5.4:17946542-17946561 None:intergenic 30.0%
! CATTTTGTGAAAGAGATGTT+AGG + chr5.4:17946037-17946056 MS.gene070105:CDS 30.0%
! CATTTTTCTTCGAATTCAGA+TGG - chr5.4:17946621-17946640 None:intergenic 30.0%
! GAGCTGCATATGAATATTTT+GGG + chr5.4:17946226-17946245 MS.gene070105:CDS 30.0%
! GATAATTTTCTCTTTGTTCG+AGG - chr5.4:17947575-17947594 None:intergenic 30.0%
! TCATGTACGAAATGTGTTTT+GGG + chr5.4:17946188-17946207 MS.gene070105:CDS 30.0%
! TGAATTCATGAAGTGTTTCA+TGG - chr5.4:17947412-17947431 None:intergenic 30.0%
! TTCATGTACGAAATGTGTTT+TGG + chr5.4:17946187-17946206 MS.gene070105:CDS 30.0%
!! AAATCTAGCTTGCTATGTTT+GGG + chr5.4:17947431-17947450 MS.gene070105:CDS 30.0%
!! AATGGAGTTGATAACTTTAG+TGG + chr5.4:17945550-17945569 MS.gene070105:CDS 30.0%
!! CTTCTAAAAAGGGTTTTGAT+TGG + chr5.4:17945653-17945672 MS.gene070105:CDS 30.0%
!! TGAAGCTGATTTTCAATCAA+TGG + chr5.4:17946881-17946900 MS.gene070105:CDS 30.0%
!! TTGAAAAAATCTTTTTGGGC+TGG + chr5.4:17946729-17946748 MS.gene070105:CDS 30.0%
AACTTAGCAGTAATCTTTGC+AGG - chr5.4:17945811-17945830 None:intergenic 35.0%
AAGAGAAGGAAATCATGTGT+CGG + chr5.4:17945738-17945757 MS.gene070105:CDS 35.0%
AGCATACATGCTTTCTTTGA+TGG + chr5.4:17946777-17946796 MS.gene070105:CDS 35.0%
AGCATTCACAACTAACATCA+TGG - chr5.4:17947136-17947155 None:intergenic 35.0%
ATCCAAATCAATGATGAAGC+GGG + chr5.4:17945952-17945971 MS.gene070105:CDS 35.0%
CAATCAACTTTACTTGGATG+TGG + chr5.4:17946333-17946352 MS.gene070105:CDS 35.0%
CACTAAGTCAAGGTTATTCA+AGG + chr5.4:17945894-17945913 MS.gene070105:CDS 35.0%
CATGGTCAATCAACTTTACT+TGG + chr5.4:17946327-17946346 MS.gene070105:CDS 35.0%
CCTGAAAAGCTTAGTAGATA+TGG + chr5.4:17946540-17946559 MS.gene070105:CDS 35.0%
CTTGAATAACCTTGACTTAG+TGG - chr5.4:17945896-17945915 None:intergenic 35.0%
GCAAAGATTACTGCTAAGTT+GGG + chr5.4:17945811-17945830 MS.gene070105:CDS 35.0%
GGCCAAATAGAATTATTCGA+AGG + chr5.4:17947492-17947511 MS.gene070105:CDS 35.0%
GTTACCTTCCACGATATTTA+AGG - chr5.4:17947027-17947046 None:intergenic 35.0%
TAGAACATCAAAGAAAGGAG+AGG + chr5.4:17945681-17945700 MS.gene070105:CDS 35.0%
TATTCCCTACTACTAGACAT+AGG + chr5.4:17946487-17946506 MS.gene070105:CDS 35.0%
TCGCATGAGTATATTCTTCT+TGG - chr5.4:17946953-17946972 None:intergenic 35.0%
TGAATAACCTTGACTTAGTG+GGG - chr5.4:17945894-17945913 None:intergenic 35.0%
TGCAAAGATTACTGCTAAGT+TGG + chr5.4:17945810-17945829 MS.gene070105:CDS 35.0%
TGTTACCTCCTTAAATATCG+TGG + chr5.4:17947016-17947035 MS.gene070105:CDS 35.0%
TTGAGCTGGAACATAATGAT+TGG + chr5.4:17946661-17946680 MS.gene070105:CDS 35.0%
! AACATAATGATTGGCTGAGT+GGG + chr5.4:17946670-17946689 MS.gene070105:CDS 35.0%
! AATAGAGAAAGGCGTATCAT+TGG + chr5.4:17946069-17946088 MS.gene070105:CDS 35.0%
! AGTAGGGAATACTGATTGAA+TGG - chr5.4:17946478-17946497 None:intergenic 35.0%
! CAAGTAAAGTTGATTGACCA+TGG - chr5.4:17946329-17946348 None:intergenic 35.0%
! CCATATCTACTAAGCTTTTC+AGG - chr5.4:17946543-17946562 None:intergenic 35.0%
! TGCTCATTGTTTGAGTTTAG+AGG + chr5.4:17947152-17947171 MS.gene070105:CDS 35.0%
!! ACTAAGCTTTTCAGGGATTT+TGG - chr5.4:17946535-17946554 None:intergenic 35.0%
!! CAAATCTAGCTTGCTATGTT+TGG + chr5.4:17947430-17947449 MS.gene070105:CDS 35.0%
!! CATTTTGCATAGCCTTACAT+TGG - chr5.4:17946455-17946474 None:intergenic 35.0%
!!! CAATTGGTCACCCTTTTTTT+TGG - chr5.4:17947636-17947655 None:intergenic 35.0%
!!! GATGTTTGTGATGGTTTTGA+TGG + chr5.4:17945520-17945539 MS.gene070105:CDS 35.0%
!!! TTGTGTGGCTTTTTCAATCA+TGG + chr5.4:17946385-17946404 MS.gene070105:CDS 35.0%
AAAAAGTTGTGCAGAGAAGC+AGG + chr5.4:17947603-17947622 MS.gene070105:CDS 40.0%
AACTTTACTTGGATGTGGTC+TGG + chr5.4:17946338-17946357 MS.gene070105:CDS 40.0%
ACACCAACAAATGCAGCAAA+AGG - chr5.4:17946300-17946319 None:intergenic 40.0%
ACATAATTGGCACCCATCTA+TGG - chr5.4:17946710-17946729 None:intergenic 40.0%
ACATCAAAGAAAGGAGAGGA+TGG + chr5.4:17945685-17945704 MS.gene070105:CDS 40.0%
ACCACCTATGTCTAGTAGTA+GGG - chr5.4:17946494-17946513 None:intergenic 40.0%
ACCTCCTTAAATATCGTGGA+AGG + chr5.4:17947020-17947039 MS.gene070105:CDS 40.0%
ACCTTCCACGATATTTAAGG+AGG - chr5.4:17947024-17947043 None:intergenic 40.0%
AGAGAAGGAAATCATGTGTC+GGG + chr5.4:17945739-17945758 MS.gene070105:CDS 40.0%
AGGAAGCGGACCAAAAAAAA+GGG + chr5.4:17947623-17947642 MS.gene070105:CDS 40.0%
AGTTGAAGCCACAAATGGAT+AGG + chr5.4:17947312-17947331 MS.gene070105:CDS 40.0%
ATCATTGGAAAAGGAGGAGA+CGG + chr5.4:17946084-17946103 MS.gene070105:CDS 40.0%
ATGCTATCTTGCTCAAGAGA+AGG + chr5.4:17945724-17945743 MS.gene070105:CDS 40.0%
ATGGACACAACAATTCCTTG+TGG + chr5.4:17946900-17946919 MS.gene070105:CDS 40.0%
CATCAAAGAAAGGAGAGGAT+GGG + chr5.4:17945686-17945705 MS.gene070105:CDS 40.0%
CTAATTGTGTGAGAGTGGTT+AGG - chr5.4:17945871-17945890 None:intergenic 40.0%
CTGTCAAACCTATCCATTTG+TGG - chr5.4:17947323-17947342 None:intergenic 40.0%
CTTGTGCAAGCATTTCTATG+AGG + chr5.4:17947343-17947362 MS.gene070105:CDS 40.0%
GAAGTTTAAGGAGGTTCAAG+CGG + chr5.4:17946971-17946990 MS.gene070105:CDS 40.0%
GTGTTGGAGGAGATGTTAAT+TGG + chr5.4:17947062-17947081 MS.gene070105:CDS 40.0%
TACTCATGCGAAGTTTAAGG+AGG + chr5.4:17946962-17946981 MS.gene070105:CDS 40.0%
TGAAGACACTTCCGACTAAA+AGG + chr5.4:17945776-17945795 MS.gene070105:CDS 40.0%
TGGACACAACAATTCCTTGT+GGG + chr5.4:17946901-17946920 MS.gene070105:CDS 40.0%
TTTCTATGAGGTTGCTGAAG+TGG + chr5.4:17947355-17947374 MS.gene070105:CDS 40.0%
TTTGTTGGTGTGAATCACCA+TGG + chr5.4:17946309-17946328 MS.gene070105:CDS 40.0%
! ATGCCTTTTGCTGCATTTGT+TGG + chr5.4:17946294-17946313 MS.gene070105:CDS 40.0%
! GAAAGGCGTATCATTGGAAA+AGG + chr5.4:17946075-17946094 MS.gene070105:CDS 40.0%
! GAACATAATGATTGGCTGAG+TGG + chr5.4:17946669-17946688 MS.gene070105:CDS 40.0%
! GATGTCCTTTTCATCTGTAG+CGG + chr5.4:17945603-17945622 MS.gene070105:CDS 40.0%
! GGACAGCTTTTCCTTTTAGT+CGG - chr5.4:17945790-17945809 None:intergenic 40.0%
!! GGAAGTGTCTTCAATGTACA+TGG - chr5.4:17945769-17945788 None:intergenic 40.0%
!!! GGTTTTGATGGTGAAGCTAA+TGG + chr5.4:17945532-17945551 MS.gene070105:CDS 40.0%
ACTATTGTTAGAGACGCTGG+AGG + chr5.4:17946000-17946019 MS.gene070105:CDS 45.0%
AGGCGTATCATTGGAAAAGG+AGG + chr5.4:17946078-17946097 MS.gene070105:CDS 45.0%
CAATTAGCCCCACTAAGTCA+AGG + chr5.4:17945884-17945903 MS.gene070105:CDS 45.0%
CACCACCTATGTCTAGTAGT+AGG - chr5.4:17946495-17946514 None:intergenic 45.0%
CACGTGCCTTAGTTGTCAAT+TGG - chr5.4:17947652-17947671 None:intergenic 45.0%
CAGACTATTGTTAGAGACGC+TGG + chr5.4:17945997-17946016 MS.gene070105:CDS 45.0%
CAGGAAGCGGACCAAAAAAA+AGG + chr5.4:17947622-17947641 MS.gene070105:CDS 45.0%
CATGGCTTCGTTGTATGCAA+GGG + chr5.4:17946403-17946422 MS.gene070105:CDS 45.0%
CCAACACGTGGTAAATAGCA+AGG - chr5.4:17947049-17947068 None:intergenic 45.0%
CTAGACATAGGTGGTGTTTG+TGG + chr5.4:17946499-17946518 MS.gene070105:CDS 45.0%
CTGGTATGTCGACAACACAA+CGG + chr5.4:17946748-17946767 MS.gene070105:CDS 45.0%
GAAAAGGACATCCCTGATGA+AGG - chr5.4:17945595-17945614 None:intergenic 45.0%
GACAGAGTTGAAGCCACAAA+TGG + chr5.4:17947307-17947326 MS.gene070105:CDS 45.0%
GGGTGACCAATTGACAACTA+AGG + chr5.4:17947643-17947662 MS.gene070105:CDS 45.0%
GTACAATGAGCGCCATAGAT+GGG + chr5.4:17946695-17946714 MS.gene070105:CDS 45.0%
TCATGGCTTCGTTGTATGCA+AGG + chr5.4:17946402-17946421 MS.gene070105:CDS 45.0%
TCCCTACTACTAGACATAGG+TGG + chr5.4:17946490-17946509 MS.gene070105:CDS 45.0%
TGGTCAGTGATAATACCTGC+AGG - chr5.4:17946435-17946454 None:intergenic 45.0%
TGTACAATGAGCGCCATAGA+TGG + chr5.4:17946694-17946713 MS.gene070105:CDS 45.0%
TTAACATCTCCTCCAACACG+TGG - chr5.4:17947061-17947080 None:intergenic 45.0%
! AAGACACGGAGTCTTTTGTG+TGG + chr5.4:17946370-17946389 MS.gene070105:CDS 45.0%
! TCTGGTTTCAGCTGAAGACA+CGG + chr5.4:17946356-17946375 MS.gene070105:CDS 45.0%
! TGCTATTTACCACGTGTTGG+AGG + chr5.4:17947049-17947068 MS.gene070105:CDS 45.0%
!! CCTTGCTATTTACCACGTGT+TGG + chr5.4:17947046-17947065 MS.gene070105:CDS 45.0%
ATCGATGAGCTTGACCCACA+AGG - chr5.4:17946918-17946937 None:intergenic 50.0%
ATTTGGCCTCGAGTCAGTGT+TGG - chr5.4:17947480-17947499 None:intergenic 50.0%
CAAGTTGCGAGCCTTCATCA+GGG + chr5.4:17945581-17945600 MS.gene070105:CDS 50.0%
CACCTCCGCTACAGATGAAA+AGG - chr5.4:17945611-17945630 None:intergenic 50.0%
GCTTCGTTGTATGCAAGGGA+AGG + chr5.4:17946407-17946426 MS.gene070105:CDS 50.0%
TGGGGCTAATTGTGTGAGAG+TGG - chr5.4:17945876-17945895 None:intergenic 50.0%
TTGTGCAGAGAAGCAGGAAG+CGG + chr5.4:17947609-17947628 MS.gene070105:CDS 50.0%
! CGCCCGCTTCATCATTGATT+TGG - chr5.4:17945957-17945976 None:intergenic 50.0%
! GTCCTTTTCATCTGTAGCGG+AGG + chr5.4:17945606-17945625 MS.gene070105:CDS 50.0%
!! GAAGTTCCAACACTGACTCG+AGG + chr5.4:17947471-17947490 MS.gene070105:CDS 50.0%
CCATTGCGAGTCACGCGTAA+AGG + chr5.4:17947545-17947564 MS.gene070105:CDS 55.0%
CCTTTACGCGTGACTCGCAA+TGG - chr5.4:17947548-17947567 None:intergenic 55.0%
GCAAGTTGCGAGCCTTCATC+AGG + chr5.4:17945580-17945599 MS.gene070105:CDS 55.0%
TCAATGATGAAGCGGGCGTG+AGG + chr5.4:17945959-17945978 MS.gene070105:CDS 55.0%
!! GCGTGACTCGCAATGGACTA+CGG - chr5.4:17947541-17947560 None:intergenic 55.0%
ATGCAAGGGAAGGCTCCTGC+AGG + chr5.4:17946417-17946436 MS.gene070105:CDS 60.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 17945499 17947709 17945499 ID=MS.gene070105
chr5.4 mRNA 17945499 17947709 17945499 ID=MS.gene070105.t1;Parent=MS.gene070105
chr5.4 exon 17945499 17947709 17945499 ID=MS.gene070105.t1.exon1;Parent=MS.gene070105.t1
chr5.4 CDS 17945499 17947709 17945499 ID=cds.MS.gene070105.t1;Parent=MS.gene070105.t1
Gene Sequence

>MS.gene070105

ATGGATAGTCCAAACAATTTTGATGTTTGTGATGGTTTTGATGGTGAAGCTAATGGAGTTGATAACTTTAGTGGTGAACAAGCAAGTTGCGAGCCTTCATCAGGGATGTCCTTTTCATCTGTAGCGGAGGTGAAATCATTTTATAAAGAATATGCTTCTAAAAAGGGTTTTGATTGGAAGATTAGAACATCAAAGAAAGGAGAGGATGGGAATCTCAAGTACTTCATGCTATCTTGCTCAAGAGAAGGAAATCATGTGTCGGGAGTACCATGTACATTGAAGACACTTCCGACTAAAAGGAAAAGCTGTCCTGCAAAGATTACTGCTAAGTTGGGAAAAGATGAATTATGGTATATTAAAAAGTTTGAACCTAACCACTCTCACACAATTAGCCCCACTAAGTCAAGGTTATTCAAGGCAAACAAAAATATGAACATGCATGCAAAGAGAACAATCCAAATCAATGATGAAGCGGGCGTGAGGATCAACAAGACTTTTCAGACTATTGTTAGAGACGCTGGAGGTTATGAAAATGTAGCATTTTGTGAAAGAGATGTTAGGAATTATGTTAATAGAGAAAGGCGTATCATTGGAAAAGGAGGAGACGGCAAAGCATTGCTAAATTATTTTTGTCGAATGAGAGAGCAAAATACAAATTTCTTCTATGATATAGATTTGGATGAAGATTTTCATGTACGAAATGTGTTTTGGGCTGATGCAAGAAGCAGAGCTGCATATGAATATTTTGGGGATGTTGTGACTTTCGATACAACGTATTTGACCAATAAATATGATATGCCTTTTGCTGCATTTGTTGGTGTGAATCACCATGGTCAATCAACTTTACTTGGATGTGGTCTGGTTTCAGCTGAAGACACGGAGTCTTTTGTGTGGCTTTTTCAATCATGGCTTCGTTGTATGCAAGGGAAGGCTCCTGCAGGTATTATCACTGACCAATGTAAGGCTATGCAAAATGCCATTCAATCAGTATTCCCTACTACTAGACATAGGTGGTGTTTGTGGCACATAATGACCAAAATCCCTGAAAAGCTTAGTAGATATGGTAAATATAAGAATATTAAGTTTGCAATGAAAGAAGCAGTTTATGATTCACTTACACCATCTGAATTCGAAGAAAAATGGTGTTATTTTGTTAACAAATTTGAGCTGGAACATAATGATTGGCTGAGTGGGTTGTACAATGAGCGCCATAGATGGGTGCCAATTATGTTGAAAAAATCTTTTTGGGCTGGTATGTCGACAACACAACGGAGTGAGAGCATACATGCTTTCTTTGATGGATATATCAATTCGACAACCAGTTTGCATCAATTTGTAAATCAATATGATAATGCTCTCAAAAGTAGAGCAGAAAAAGAATTTGAAGCTGATTTTCAATCAATGGACACAACAATTCCTTGTGGGTCAAGCTCATCGATCGAAAAGCAATTCCAAGAAGAATATACTCATGCGAAGTTTAAGGAGGTTCAAGCGGAATTCAGAGCTAAAATGAATTGTGTTACCTCCTTAAATATCGTGGAAGGTAACCTTGCTATTTACCACGTGTTGGAGGAGATGTTAATTGGAGATAGAAGAAAAGATCGTATCCTTAAAGTTTCTTTTGATCGTGACAGCCATGATGTTAGTTGTGAATGCTCATTGTTTGAGTTTAGAGGTATTGTATGTCGTCATGTGTTATCTGTGTGTGCTCAAGAAAGAGTTAAAAACATGCCACTGAAGTATGTTTTAGTAAGATGGAGCAAAAATATTAAAAGGAAGCATTCATATATTAAGAGTAGCTATAATGTGACAGAGTTGAAGCCACAAATGGATAGGTTTGACAGCTTGTGCAAGCATTTCTATGAGGTTGCTGAAGTGGCTGCAGAAAAAGAAGATAAAACTATGGCTCTCCATGAAACACTTCATGAATTCAAATCTAGCTTGCTATGTTTGGGTGCTACAAGTAACAACAGAAGTTCCAACACTGACTCGAGGCCAAATAGAATTATTCGAAGGGATGAAGATGCGATTATTGAAATCCGTAGTCCATTGCGAGTCACGCGTAAAGGTCGTCCTCGAACAAAGAGAAAATTATCTTTAGTTGAAAAAGTTGTGCAGAGAAGCAGGAAGCGGACCAAAAAAAAGGGTGACCAATTGACAACTAAGGCACGTGATTATTTTCATAATATATGCATTAAAATGAAGTATTAA
Protein sequence

>MS.gene070105.t1

MDSPNNFDVCDGFDGEANGVDNFSGEQASCEPSSGMSFSSVAEVKSFYKEYASKKGFDWKIRTSKKGEDGNLKYFMLSCSREGNHVSGVPCTLKTLPTKRKSCPAKITAKLGKDELWYIKKFEPNHSHTISPTKSRLFKANKNMNMHAKRTIQINDEAGVRINKTFQTIVRDAGGYENVAFCERDVRNYVNRERRIIGKGGDGKALLNYFCRMREQNTNFFYDIDLDEDFHVRNVFWADARSRAAYEYFGDVVTFDTTYLTNKYDMPFAAFVGVNHHGQSTLLGCGLVSAEDTESFVWLFQSWLRCMQGKAPAGIITDQCKAMQNAIQSVFPTTRHRWCLWHIMTKIPEKLSRYGKYKNIKFAMKEAVYDSLTPSEFEEKWCYFVNKFELEHNDWLSGLYNERHRWVPIMLKKSFWAGMSTTQRSESIHAFFDGYINSTTSLHQFVNQYDNALKSRAEKEFEADFQSMDTTIPCGSSSSIEKQFQEEYTHAKFKEVQAEFRAKMNCVTSLNIVEGNLAIYHVLEEMLIGDRRKDRILKVSFDRDSHDVSCECSLFEFRGIVCRHVLSVCAQERVKNMPLKYVLVRWSKNIKRKHSYIKSSYNVTELKPQMDRFDSLCKHFYEVAEVAAEKEDKTMALHETLHEFKSSLLCLGATSNNRSSNTDSRPNRIIRRDEDAIIEIRSPLRVTRKGRPRTKRKLSLVEKVVQRSRKRTKKKGDQLTTKARDYFHNICIKMKY