AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070127

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070127.t1 MTR_5g027570 64.444 225 46 5 114 308 11 231 2.63e-87 261
MS.gene070127.t1 MTR_5g027550 65.909 220 49 5 114 308 11 229 1.76e-84 254
MS.gene070127.t1 MTR_5g088060 62.927 205 45 5 113 291 199 398 5.18e-74 234
MS.gene070127.t1 MTR_5g488160 62.927 205 45 5 113 291 199 398 5.18e-74 234
MS.gene070127.t1 MTR_5g088010 60.215 186 48 4 132 291 1 186 2.79e-64 203
MS.gene070127.t1 MTR_5g027780 70.290 138 31 1 20 147 36 173 6.59e-59 187
MS.gene070127.t1 MTR_5g027770 51.442 208 40 7 1 185 1 170 2.12e-45 156
MS.gene070127.t1 MTR_5g027770 58.140 129 18 1 21 113 171 299 3.33e-36 132
MS.gene070127.t1 MTR_5g088200 47.644 191 26 5 1 117 1 191 7.16e-39 145
MS.gene070127.t1 MTR_5g088005 58.519 135 30 3 1 117 1 127 2.53e-35 125
MS.gene070127.t1 MTR_3g462790 44.444 126 65 3 140 262 104 227 2.62e-22 95.1
MS.gene070127.t1 MTR_8g063600 54.217 83 37 1 177 259 102 183 5.07e-22 94.0
MS.gene070127.t1 MTR_1g111830 41.538 130 69 3 146 275 93 215 1.98e-21 91.3
MS.gene070127.t1 MTR_5g081860 51.000 100 44 2 157 255 105 200 2.21e-21 91.7
MS.gene070127.t1 MTR_5g069710 46.774 124 54 5 143 265 103 215 3.99e-21 91.7
MS.gene070127.t1 MTR_8g063870 55.128 78 34 1 182 259 93 169 6.74e-21 90.5
MS.gene070127.t1 MTR_2g100930 46.316 95 49 1 177 271 105 197 8.54e-21 91.7
MS.gene070127.t1 MTR_4g100630 34.932 146 70 4 146 277 47 181 3.94e-20 89.4
MS.gene070127.t1 MTR_4g111975 51.250 80 36 2 177 255 134 211 8.40e-20 88.2
MS.gene070127.t1 MTR_7g067080 52.500 80 36 1 170 249 129 206 2.20e-19 87.0
MS.gene070127.t1 MTR_4g107230 36.747 166 92 4 118 283 25 177 4.19e-19 85.9
MS.gene070127.t1 MTR_1g048660 53.425 73 32 1 183 255 14 84 5.04e-19 80.5
MS.gene070127.t1 MTR_3g104370 43.956 91 43 2 183 265 93 183 1.34e-17 80.5
MS.gene070127.t1 MTR_6g092540 52.857 70 31 1 177 246 93 160 5.14e-17 80.1
MS.gene070127.t1 MTR_8g101650 52.857 70 30 1 177 246 101 167 5.81e-17 79.7
MS.gene070127.t1 MTR_5g037080 62.712 59 18 1 170 228 95 149 9.25e-17 79.7
MS.gene070127.t1 MTR_4g015130 50.725 69 31 2 191 258 3 69 1.95e-16 73.6
MS.gene070127.t1 MTR_5g075760 47.727 88 38 4 182 267 106 187 3.58e-16 76.3
MS.gene070127.t1 MTR_1g083180 50.649 77 32 3 181 254 61 134 2.43e-15 73.2
MS.gene070127.t1 MTR_5g075790 37.190 121 64 5 153 267 81 195 8.54e-15 72.8
MS.gene070127.t1 MTR_1g033620 55.738 61 24 2 196 255 20 78 1.49e-14 72.4
MS.gene070127.t1 MTR_0036s0260 41.892 74 41 1 178 251 25 96 7.93e-14 69.7
MS.gene070127.t1 MTR_2g090305 44.928 69 36 1 183 251 30 96 1.31e-13 68.6
MS.gene070127.t1 MTR_3g070110 51.389 72 30 1 182 248 96 167 1.63e-13 68.9
MS.gene070127.t1 MTR_8g072990 40.260 77 39 1 166 242 92 161 1.53e-11 63.5
MS.gene070127.t1 MTR_6g043490 40.541 74 41 1 181 251 107 180 5.70e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070127.t1 AT5G08520 45.045 111 57 2 140 248 75 183 3.67e-22 94.4
MS.gene070127.t1 AT5G58900 52.381 105 35 5 147 250 119 209 2.53e-21 92.0
MS.gene070127.t1 AT1G49010 49.451 91 43 2 173 262 126 214 4.05e-21 92.0
MS.gene070127.t1 AT5G04760 56.250 80 29 2 169 248 91 164 5.64e-21 89.4
MS.gene070127.t1 AT5G23650 43.860 114 60 2 144 257 85 194 3.16e-20 89.7
MS.gene070127.t1 AT3G10580 44.898 98 47 3 165 260 77 169 5.61e-20 87.8
MS.gene070127.t1 AT3G10580 44.898 98 47 3 165 260 77 169 7.16e-20 88.2
MS.gene070127.t1 AT2G38090 52.326 86 38 2 170 254 130 213 1.01e-19 87.8
MS.gene070127.t1 AT3G11280 51.111 90 38 2 167 252 113 200 1.15e-19 87.0
MS.gene070127.t1 AT3G11280 51.111 90 38 2 167 252 113 200 1.15e-19 87.0
MS.gene070127.t1 AT5G05790 46.465 99 50 2 166 263 116 212 1.41e-19 87.0
MS.gene070127.t1 AT5G05790 46.465 99 50 2 166 263 116 212 1.41e-19 87.0
MS.gene070127.t1 AT5G61620 47.525 101 39 3 170 262 101 195 2.33e-19 87.0
MS.gene070127.t1 AT5G01200 53.846 78 30 2 171 248 140 211 4.95e-19 85.5
MS.gene070127.t1 AT5G47390 51.948 77 31 2 170 246 89 159 2.32e-18 84.7
MS.gene070127.t1 AT5G56840 54.286 70 31 1 177 246 87 155 7.42e-17 78.6
MS.gene070127.t1 AT4G09450 47.619 84 42 1 166 249 76 157 1.08e-16 77.4
MS.gene070127.t1 AT1G19000 46.667 90 39 2 140 227 66 148 1.18e-16 79.0
MS.gene070127.t1 AT1G19000 46.667 90 39 2 140 227 66 148 1.18e-16 79.0
MS.gene070127.t1 AT1G74840 56.250 64 28 0 164 227 79 142 1.97e-16 77.4
MS.gene070127.t1 AT1G74840 56.250 64 28 0 164 227 79 142 2.40e-16 77.8
MS.gene070127.t1 AT1G70000 60.345 58 19 1 171 228 92 145 3.90e-16 77.0
MS.gene070127.t1 AT1G70000 60.345 58 19 1 171 228 92 145 3.90e-16 77.0
MS.gene070127.t1 AT3G10590 35.374 147 74 4 124 269 67 193 1.03e-15 74.7
MS.gene070127.t1 AT3G16350 47.368 76 40 0 153 228 109 184 1.11e-15 77.4

Find 80 sgRNAs with CRISPR-Local

Find 126 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCTCTGTATCTTTCACATTT+TGG 0.106986 5.4:-18268151 None:intergenic
TGATGTGTCATTCTTGTTTA+AGG 0.162194 5.4:-18268207 None:intergenic
TGTGATGTATAGGTTGTTTC+TGG 0.210646 5.4:+18268019 MS.gene070127:intron
TCAACACGATTCTTAGGTTT+TGG 0.247019 5.4:-18266668 None:intergenic
ACATTCTCAACACGATTCTT+AGG 0.260608 5.4:-18266674 None:intergenic
TTCTCAAGATTGTTCATATC+AGG 0.300574 5.4:-18268329 None:intergenic
CGAACATTGCGCTTGGGGCC+TGG 0.318948 5.4:-18266728 None:intergenic
TTTGCTTCACGACTTCCTTC+TGG 0.354439 5.4:-18267397 None:intergenic
GCGCAACTTGTTCAACTTGT+TGG 0.354873 5.4:+18268355 MS.gene070127:CDS
GGATTCTCACCATTCGAATA+AGG 0.364094 5.4:-18266812 None:intergenic
GATGTTCCTGTGTTGGTTGT+TGG 0.372353 5.4:+18266608 MS.gene070127:CDS
TATACTTTCCCACCTGGTTC+AGG 0.373635 5.4:+18266788 MS.gene070127:CDS
CCTGCTGCCACAGGGGATAC+AGG 0.376950 5.4:-18266839 None:intergenic
TGCTATGCTCAGAGGGATCT+TGG 0.382368 5.4:-18267270 None:intergenic
CATTTAAATCAATTTGATGT+AGG 0.397989 5.4:-18267234 None:intergenic
ACGGGCCGAACATTGCGCTT+GGG 0.403809 5.4:-18266734 None:intergenic
CACGGGCCGAACATTGCGCT+TGG 0.408670 5.4:-18266735 None:intergenic
GCACCTTGTCCTGCTGCCAC+AGG 0.438140 5.4:-18266848 None:intergenic
CAAAGAGAGGGCGTAGTGAA+TGG 0.443133 5.4:+18267416 MS.gene070127:CDS
CAGGCCCCAAGCGCAATGTT+CGG 0.443678 5.4:+18266729 MS.gene070127:CDS
CACCTTGTCCTGCTGCCACA+GGG 0.446495 5.4:-18266847 None:intergenic
CACATCAGTCACTGCTCCTA+TGG 0.448672 5.4:+18268223 MS.gene070127:CDS
AGATATTTCAAAAGAATTTG+TGG 0.448838 5.4:+18268076 MS.gene070127:CDS
GAACAACAACAGGAGAAAAC+TGG 0.454055 5.4:+18267295 MS.gene070127:CDS
GGTTTCTTCCATGTAGACTT+AGG 0.456119 5.4:-18266563 None:intergenic
GCGTAGTGAATGGGTACATT+GGG 0.473170 5.4:+18267426 MS.gene070127:CDS
CCTGCATATACTTTCCCACC+TGG 0.477058 5.4:+18266782 MS.gene070127:CDS
CCTGTATCCCCTGTGGCAGC+AGG 0.482180 5.4:+18266839 MS.gene070127:CDS
GCATAGCATGGAACAACAAC+AGG 0.485938 5.4:+18267285 MS.gene070127:CDS
TTGGTGCTGCTCTGTCCAAT+CGG 0.496082 5.4:+18268296 MS.gene070127:CDS
TCAAATTGATTTAAATGAGT+CGG 0.503726 5.4:+18267240 MS.gene070127:CDS
TGATAGCGTGGTGATGCCAC+AGG 0.503741 5.4:-18266701 None:intergenic
CGGGCCGAACATTGCGCTTG+GGG 0.503942 5.4:-18266733 None:intergenic
CTTGAGGTGGCTGTGTCTTG+AGG 0.504065 5.4:-18268263 None:intergenic
GGATTCTCATGTTGTTCCAT+AGG 0.504821 5.4:-18268239 None:intergenic
ATGTGAGTTGGAGGTGTCAC+GGG 0.506569 5.4:-18266752 None:intergenic
CACCAACTGCATTTGTCTTG+AGG 0.508085 5.4:-18268279 None:intergenic
GGCAGCGATATCAATGAGTC+GGG 0.525254 5.4:+18267316 MS.gene070127:CDS
GCATGGCTAGCAATTTGAGT+TGG 0.525594 5.4:-18268110 None:intergenic
AAAGAGAGGGCGTAGTGAAT+GGG 0.526290 5.4:+18267417 MS.gene070127:CDS
TCCCCTGTGGCAGCAGGACA+AGG 0.527271 5.4:+18266845 MS.gene070127:CDS
TGTATAGGTTGTTTCTGGAG+GGG 0.528745 5.4:+18268024 MS.gene070127:intron
AGAAAGAGCTGGCCAAGACT+TGG 0.529628 5.4:-18266633 None:intergenic
GGCGTAGTGAATGGGTACAT+TGG 0.531028 5.4:+18267425 MS.gene070127:CDS
GAATGGGTACATTGGGCAGA+AGG 0.535871 5.4:+18267433 MS.gene070127:CDS
TGTTCCATGCTATGCTCAGA+GGG 0.536332 5.4:-18267277 None:intergenic
GCCACAGGGGATACAGGCTT+AGG 0.537409 5.4:-18266833 None:intergenic
ATGTATAGGTTGTTTCTGGA+GGG 0.542704 5.4:+18268023 MS.gene070127:intron
CAACTGCATTTGTCTTGAGG+TGG 0.545901 5.4:-18268276 None:intergenic
GCAGGAACAGCAATGTGAGT+TGG 0.551521 5.4:-18266764 None:intergenic
TGGTTGTTGGAACCAAGTCT+TGG 0.553347 5.4:+18266621 MS.gene070127:CDS
TTCGAATAAGGTCCTGAACC+AGG 0.561660 5.4:-18266800 None:intergenic
TCACCACGCTATCAAAAGCC+AGG 0.565310 5.4:+18266710 MS.gene070127:CDS
AATGTGAGTTGGAGGTGTCA+CGG 0.566092 5.4:-18266753 None:intergenic
TTGTTCCATGCTATGCTCAG+AGG 0.567759 5.4:-18267278 None:intergenic
CACCTCAAGACAAATGCAGT+TGG 0.567812 5.4:+18268277 MS.gene070127:CDS
TGGCAGCGATATCAATGAGT+CGG 0.568863 5.4:+18267315 MS.gene070127:CDS
GAGGTGGCTGTGTCTTGAGG+AGG 0.576603 5.4:-18268260 None:intergenic
TCAGGAAGTGTAGAACCGAT+TGG 0.579196 5.4:-18268311 None:intergenic
AAGTGTTGATGTTCCTGTGT+TGG 0.580284 5.4:+18266601 MS.gene070127:CDS
GTTTGAGCAACAGAAAGAGC+TGG 0.581931 5.4:-18266644 None:intergenic
TTAGTGCTCCTAAGTCTACA+TGG 0.585666 5.4:+18266555 MS.gene070127:CDS
CCAGGTGGGAAAGTATATGC+AGG 0.588904 5.4:-18266782 None:intergenic
GGTTCAGGACCTTATTCGAA+TGG 0.589457 5.4:+18266803 MS.gene070127:CDS
TCTTGTTTAAGGTTGTGTCG+TGG 0.595697 5.4:-18268196 None:intergenic
AGATCCCTCTGAGCATAGCA+TGG 0.596307 5.4:+18267273 MS.gene070127:CDS
ATGTGAAAGATACAGAGAAG+AGG 0.601303 5.4:+18268156 MS.gene070127:CDS
ATTAGAGTTTAGAATTGCGA+CGG 0.604472 5.4:-18267344 None:intergenic
GATGTATAGGTTGTTTCTGG+AGG 0.607647 5.4:+18268022 MS.gene070127:intron
GGAACAGCAATGTGAGTTGG+AGG 0.613590 5.4:-18266761 None:intergenic
AAGTCTACATGGAAGAAACC+TGG 0.626539 5.4:+18266566 MS.gene070127:CDS
CAAGCTGAGTCAGTACCAGA+AGG 0.630435 5.4:+18267382 MS.gene070127:CDS
GGAAGTCGTGAAGCAAAGAG+AGG 0.632062 5.4:+18267403 MS.gene070127:CDS
GAAGTCGTGAAGCAAAGAGA+GGG 0.647998 5.4:+18267404 MS.gene070127:CDS
AATAAGGTCCTGAACCAGGT+GGG 0.650251 5.4:-18266796 None:intergenic
TGTTGAGAATGTTCGTCCTG+TGG 0.664926 5.4:+18266685 MS.gene070127:CDS
TTGGTTCCAACAACCAACAC+AGG 0.665985 5.4:-18266614 None:intergenic
TCCTAAGCCTGTATCCCCTG+TGG 0.686944 5.4:+18266832 MS.gene070127:CDS
ACCTTGTCCTGCTGCCACAG+GGG 0.694899 5.4:-18266846 None:intergenic
GAATAAGGTCCTGAACCAGG+TGG 0.699303 5.4:-18266797 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATTGTAAATACATATCAAT+TGG - chr5.4:18267706-18267725 None:intergenic 15.0%
!!! AAATTCTTATAGTTGTTTTT+TGG + chr5.4:18267199-18267218 MS.gene070127:intron 15.0%
!! AAAAAAAGATATAACTTCCA+AGG - chr5.4:18267485-18267504 None:intergenic 20.0%
!! AACCTAAAATAGAATTGAAA+AGG - chr5.4:18267508-18267527 None:intergenic 20.0%
!! AGATATTTCAAAAGAATTTG+TGG + chr5.4:18268076-18268095 MS.gene070127:CDS 20.0%
!! CATTTAAATCAATTTGATGT+AGG - chr5.4:18267237-18267256 None:intergenic 20.0%
!! GAAGAAATAATAAAGAAATG+AGG - chr5.4:18267952-18267971 None:intergenic 20.0%
!! TTCAATTAAGAAGATTTAAC+TGG + chr5.4:18267119-18267138 MS.gene070127:intron 20.0%
!!! ATTTTGTTAACTCACATAAT+TGG + chr5.4:18267017-18267036 MS.gene070127:intron 20.0%
!!! CATGCATAATATTTTTTGAA+TGG - chr5.4:18267162-18267181 None:intergenic 20.0%
!!! TCAAATTGATTTAAATGAGT+CGG + chr5.4:18267240-18267259 MS.gene070127:CDS 20.0%
!!! TTCCTTTTCAATTCTATTTT+AGG + chr5.4:18267503-18267522 MS.gene070127:intron 20.0%
!!! TTCTATAATTTGTGATGTAT+AGG + chr5.4:18268009-18268028 MS.gene070127:intron 20.0%
! AAGCAAGAATATTGAAGATT+AGG - chr5.4:18266879-18266898 None:intergenic 25.0%
! AGAAGCAAATTAATCTTTCT+AGG + chr5.4:18267822-18267841 MS.gene070127:intron 25.0%
! AGCAAGAATATTGAAGATTA+GGG - chr5.4:18266878-18266897 None:intergenic 25.0%
! TGTGAGTTAACAAAATTACT+TGG - chr5.4:18267013-18267032 None:intergenic 25.0%
!! TCTCATTATGTTTCTGTAAT+TGG + chr5.4:18266974-18266993 MS.gene070127:intron 25.0%
!!! TTGGTGGATAAAGTATTTTT+GGG - chr5.4:18268135-18268154 None:intergenic 25.0%
!!! TTTGGTGGATAAAGTATTTT+TGG - chr5.4:18268136-18268155 None:intergenic 25.0%
AGTGACTAGATACTAAACTT+TGG + chr5.4:18267625-18267644 MS.gene070127:intron 30.0%
GAAATGAGGTAACTATATCT+TGG - chr5.4:18267938-18267957 None:intergenic 30.0%
GTAAGTATTCAATCTATCCT+TGG + chr5.4:18267465-18267484 MS.gene070127:intron 30.0%
TTCTCAAGATTGTTCATATC+AGG - chr5.4:18268332-18268351 None:intergenic 30.0%
TTGGTATGTATCCAAAACAA+TGG - chr5.4:18267919-18267938 None:intergenic 30.0%
! ATTAGAGTTTAGAATTGCGA+CGG - chr5.4:18267347-18267366 None:intergenic 30.0%
! TCTCTGTATCTTTCACATTT+TGG - chr5.4:18268154-18268173 None:intergenic 30.0%
! TGATGTGTCATTCTTGTTTA+AGG - chr5.4:18268210-18268229 None:intergenic 30.0%
!! AGGCATAGTTGATTTGATTA+AGG + chr5.4:18267056-18267075 MS.gene070127:intron 30.0%
!! CTGTAATTGGTTTGAGTAAT+TGG + chr5.4:18266987-18267006 MS.gene070127:intron 30.0%
ACATTCTCAACACGATTCTT+AGG - chr5.4:18266677-18266696 None:intergenic 35.0%
ATGTGAAAGATACAGAGAAG+AGG + chr5.4:18268156-18268175 MS.gene070127:CDS 35.0%
TTGCTTTGATACCATTGAAG+TGG + chr5.4:18266893-18266912 MS.gene070127:intron 35.0%
! CATATCAATTGGTAGCTAGA+AGG - chr5.4:18267695-18267714 None:intergenic 35.0%
! CTGTATCTTTCACATTTTGG+TGG - chr5.4:18268151-18268170 None:intergenic 35.0%
! GATACTAAACTTTGGAGAAG+TGG + chr5.4:18267633-18267652 MS.gene070127:intron 35.0%
! TCAACACGATTCTTAGGTTT+TGG - chr5.4:18266671-18266690 None:intergenic 35.0%
! TGCAAGTGTTTCCATTGTTT+TGG + chr5.4:18267905-18267924 MS.gene070127:intron 35.0%
! TGTGATGTATAGGTTGTTTC+TGG + chr5.4:18268019-18268038 MS.gene070127:intron 35.0%
! TTGGCTAGTTGATCTGATTA+AGG + chr5.4:18267036-18267055 MS.gene070127:intron 35.0%
!! ATGTATAGGTTGTTTCTGGA+GGG + chr5.4:18268023-18268042 MS.gene070127:intron 35.0%
!!! GCATCGTTTTTTGAGTAAAC+CGG + chr5.4:18267078-18267097 MS.gene070127:intron 35.0%
!!! GGATAAAGTATTTTTGGGCA+TGG - chr5.4:18268130-18268149 None:intergenic 35.0%
!!! TTTTTTGAGTAAACCGGATG+AGG + chr5.4:18267084-18267103 MS.gene070127:intron 35.0%
AAGTCTACATGGAAGAAACC+TGG + chr5.4:18266566-18266585 MS.gene070127:CDS 40.0%
CCAACTAAAAGCCACTTCAA+TGG - chr5.4:18266907-18266926 None:intergenic 40.0%
GAACAACAACAGGAGAAAAC+TGG + chr5.4:18267295-18267314 MS.gene070127:CDS 40.0%
GGATTCTCACCATTCGAATA+AGG - chr5.4:18266815-18266834 None:intergenic 40.0%
GGATTCTCATGTTGTTCCAT+AGG - chr5.4:18268242-18268261 None:intergenic 40.0%
GGTTTCTTCCATGTAGACTT+AGG - chr5.4:18266566-18266585 None:intergenic 40.0%
TTAGTGCTCCTAAGTCTACA+TGG + chr5.4:18266555-18266574 MS.gene070127:CDS 40.0%
! ACACTTTTCTCATCAACACC+AGG - chr5.4:18266587-18266606 None:intergenic 40.0%
! CCATTGAAGTGGCTTTTAGT+TGG + chr5.4:18266904-18266923 MS.gene070127:intron 40.0%
! TCTTGTTTAAGGTTGTGTCG+TGG - chr5.4:18268199-18268218 None:intergenic 40.0%
!! AAGTGTTGATGTTCCTGTGT+TGG + chr5.4:18266601-18266620 MS.gene070127:CDS 40.0%
!! GATGTATAGGTTGTTTCTGG+AGG + chr5.4:18268022-18268041 MS.gene070127:intron 40.0%
!! TGTATAGGTTGTTTCTGGAG+GGG + chr5.4:18268024-18268043 MS.gene070127:intron 40.0%
AAAAACATGGTAGGGGACGT+TGG + chr5.4:18268051-18268070 MS.gene070127:CDS 45.0%
AATAAGGTCCTGAACCAGGT+GGG - chr5.4:18266799-18266818 None:intergenic 45.0%
AGGGGCTTGAAAAACATGGT+AGG + chr5.4:18268042-18268061 MS.gene070127:CDS 45.0%
CAACTGCATTTGTCTTGAGG+TGG - chr5.4:18268279-18268298 None:intergenic 45.0%
CACCAACTGCATTTGTCTTG+AGG - chr5.4:18268282-18268301 None:intergenic 45.0%
CACCTCAAGACAAATGCAGT+TGG + chr5.4:18268277-18268296 MS.gene070127:CDS 45.0%
GAAGGACGTTGATCTACATG+CGG - chr5.4:18267677-18267696 None:intergenic 45.0%
GAAGTCGTGAAGCAAAGAGA+GGG + chr5.4:18267404-18267423 MS.gene070127:CDS 45.0%
GCATAGCATGGAACAACAAC+AGG + chr5.4:18267285-18267304 MS.gene070127:CDS 45.0%
GCATGGCTAGCAATTTGAGT+TGG - chr5.4:18268113-18268132 None:intergenic 45.0%
GCGTAGTGAATGGGTACATT+GGG + chr5.4:18267426-18267445 MS.gene070127:CDS 45.0%
GGGCTTGAAAAACATGGTAG+GGG + chr5.4:18268044-18268063 MS.gene070127:CDS 45.0%
GGGGCTTGAAAAACATGGTA+GGG + chr5.4:18268043-18268062 MS.gene070127:CDS 45.0%
GGTTCAGGACCTTATTCGAA+TGG + chr5.4:18266803-18266822 MS.gene070127:CDS 45.0%
GTTTGAGCAACAGAAAGAGC+TGG - chr5.4:18266647-18266666 None:intergenic 45.0%
TATACTTTCCCACCTGGTTC+AGG + chr5.4:18266788-18266807 MS.gene070127:CDS 45.0%
TGGCAGCGATATCAATGAGT+CGG + chr5.4:18267315-18267334 MS.gene070127:CDS 45.0%
TGGTTGTTGGAACCAAGTCT+TGG + chr5.4:18266621-18266640 MS.gene070127:CDS 45.0%
TGTTCCATGCTATGCTCAGA+GGG - chr5.4:18267280-18267299 None:intergenic 45.0%
TGTTGAGAATGTTCGTCCTG+TGG + chr5.4:18266685-18266704 MS.gene070127:CDS 45.0%
TTCGAATAAGGTCCTGAACC+AGG - chr5.4:18266803-18266822 None:intergenic 45.0%
TTGGTTCCAACAACCAACAC+AGG - chr5.4:18266617-18266636 None:intergenic 45.0%
TTGTTCCATGCTATGCTCAG+AGG - chr5.4:18267281-18267300 None:intergenic 45.0%
TTTGCTTCACGACTTCCTTC+TGG - chr5.4:18267400-18267419 None:intergenic 45.0%
! AAAGAGAGGGCGTAGTGAAT+GGG + chr5.4:18267417-18267436 MS.gene070127:CDS 45.0%
! GCGCAACTTGTTCAACTTGT+TGG + chr5.4:18268355-18268374 MS.gene070127:CDS 45.0%
! TCAGGAAGTGTAGAACCGAT+TGG - chr5.4:18268314-18268333 None:intergenic 45.0%
!! AATGTGAGTTGGAGGTGTCA+CGG - chr5.4:18266756-18266775 None:intergenic 45.0%
!! GATGTTCCTGTGTTGGTTGT+TGG + chr5.4:18266608-18266627 MS.gene070127:CDS 45.0%
AGAAAGAGCTGGCCAAGACT+TGG - chr5.4:18266636-18266655 None:intergenic 50.0%
AGATCCCTCTGAGCATAGCA+TGG + chr5.4:18267273-18267292 MS.gene070127:CDS 50.0%
CAAGCTGAGTCAGTACCAGA+AGG + chr5.4:18267382-18267401 MS.gene070127:CDS 50.0%
CACATCAGTCACTGCTCCTA+TGG + chr5.4:18268223-18268242 MS.gene070127:CDS 50.0%
CCTGCATATACTTTCCCACC+TGG + chr5.4:18266782-18266801 MS.gene070127:CDS 50.0%
CTGGAGGGGCTTGAAAAACA+TGG + chr5.4:18268038-18268057 MS.gene070127:CDS 50.0%
GAATAAGGTCCTGAACCAGG+TGG - chr5.4:18266800-18266819 None:intergenic 50.0%
GAATGGGTACATTGGGCAGA+AGG + chr5.4:18267433-18267452 MS.gene070127:CDS 50.0%
GCAGGAACAGCAATGTGAGT+TGG - chr5.4:18266767-18266786 None:intergenic 50.0%
GGAAGTCGTGAAGCAAAGAG+AGG + chr5.4:18267403-18267422 MS.gene070127:CDS 50.0%
GGCAGCGATATCAATGAGTC+GGG + chr5.4:18267316-18267335 MS.gene070127:CDS 50.0%
GGCGTAGTGAATGGGTACAT+TGG + chr5.4:18267425-18267444 MS.gene070127:CDS 50.0%
TCACCACGCTATCAAAAGCC+AGG + chr5.4:18266710-18266729 MS.gene070127:CDS 50.0%
TGAGTAAACCGGATGAGGCT+GGG + chr5.4:18267089-18267108 MS.gene070127:intron 50.0%
TGCTATGCTCAGAGGGATCT+TGG - chr5.4:18267273-18267292 None:intergenic 50.0%
TTGAGTAAACCGGATGAGGC+TGG + chr5.4:18267088-18267107 MS.gene070127:intron 50.0%
! CAAAGAGAGGGCGTAGTGAA+TGG + chr5.4:18267416-18267435 MS.gene070127:CDS 50.0%
! CCAGGTGGGAAAGTATATGC+AGG - chr5.4:18266785-18266804 None:intergenic 50.0%
! GGAACAGCAATGTGAGTTGG+AGG - chr5.4:18266764-18266783 None:intergenic 50.0%
!! ATGTGAGTTGGAGGTGTCAC+GGG - chr5.4:18266755-18266774 None:intergenic 50.0%
!! TTGGTGCTGCTCTGTCCAAT+CGG + chr5.4:18268296-18268315 MS.gene070127:CDS 50.0%
ACTTAGTCCCCAGCCTCATC+CGG - chr5.4:18267100-18267119 None:intergenic 55.0%
CTTGAGGTGGCTGTGTCTTG+AGG - chr5.4:18268266-18268285 None:intergenic 55.0%
GAGTAAACCGGATGAGGCTG+GGG + chr5.4:18267090-18267109 MS.gene070127:intron 55.0%
TCCTAAGCCTGTATCCCCTG+TGG + chr5.4:18266832-18266851 MS.gene070127:CDS 55.0%
! TGATAGCGTGGTGATGCCAC+AGG - chr5.4:18266704-18266723 None:intergenic 55.0%
ACCTTGTCCTGCTGCCACAG+GGG - chr5.4:18266849-18266868 None:intergenic 60.0%
ACGGGCCGAACATTGCGCTT+GGG - chr5.4:18266737-18266756 None:intergenic 60.0%
CACCTTGTCCTGCTGCCACA+GGG - chr5.4:18266850-18266869 None:intergenic 60.0%
CAGGCCCCAAGCGCAATGTT+CGG + chr5.4:18266729-18266748 MS.gene070127:CDS 60.0%
GAGGTGGCTGTGTCTTGAGG+AGG - chr5.4:18268263-18268282 None:intergenic 60.0%
GCCACAGGGGATACAGGCTT+AGG - chr5.4:18266836-18266855 None:intergenic 60.0%
!!! GGGCCTGGCTTTTGATAGCG+TGG - chr5.4:18266716-18266735 None:intergenic 60.0%
CACGGGCCGAACATTGCGCT+TGG - chr5.4:18266738-18266757 None:intergenic 65.0%
CCTGCTGCCACAGGGGATAC+AGG - chr5.4:18266842-18266861 None:intergenic 65.0%
CCTGTATCCCCTGTGGCAGC+AGG + chr5.4:18266839-18266858 MS.gene070127:CDS 65.0%
CGAACATTGCGCTTGGGGCC+TGG - chr5.4:18266731-18266750 None:intergenic 65.0%
CGGGCCGAACATTGCGCTTG+GGG - chr5.4:18266736-18266755 None:intergenic 65.0%
GCACCTTGTCCTGCTGCCAC+AGG - chr5.4:18266851-18266870 None:intergenic 65.0%
TCCCCTGTGGCAGCAGGACA+AGG + chr5.4:18266845-18266864 MS.gene070127:CDS 65.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 18266533 18268391 18266533 ID=MS.gene070127
chr5.4 mRNA 18266533 18268391 18266533 ID=MS.gene070127.t1;Parent=MS.gene070127
chr5.4 exon 18266533 18266866 18266533 ID=MS.gene070127.t1.exon1;Parent=MS.gene070127.t1
chr5.4 CDS 18266533 18266866 18266533 ID=cds.MS.gene070127.t1;Parent=MS.gene070127.t1
chr5.4 exon 18267233 18267464 18267233 ID=MS.gene070127.t1.exon2;Parent=MS.gene070127.t1
chr5.4 CDS 18267233 18267464 18267233 ID=cds.MS.gene070127.t1;Parent=MS.gene070127.t1
chr5.4 exon 18268031 18268391 18268031 ID=MS.gene070127.t1.exon3;Parent=MS.gene070127.t1
chr5.4 CDS 18268031 18268391 18268031 ID=cds.MS.gene070127.t1;Parent=MS.gene070127.t1
Gene Sequence

>MS.gene070127

ATGGTGATTGCTGATAATGAGATTAGTGCTCCTAAGTCTACATGGAAGAAACCTGGTGTTGATGAGAAAAGTGTTGATGTTCCTGTGTTGGTTGTTGGAACCAAGTCTTGGCCAGCTCTTTCTGTTGCTCAAACTCCAAAACCTAAGAATCGTGTTGAGAATGTTCGTCCTGTGGCATCACCACGCTATCAAAAGCCAGGCCCCAAGCGCAATGTTCGGCCCGTGACACCTCCAACTCACATTGCTGTTCCTGCATATACTTTCCCACCTGGTTCAGGACCTTATTCGAATGGTGAGAATCCTAAGCCTGTATCCCCTGTGGCAGCAGGACAAGGTGCGAATCCCTAATCTTCAATATTCTTGCTTTGATACCATTGAAGTGGCTTTTAGTTGGAACATGATGTTTTACAATGAGTGTTTTTTATTGTCATTATGATATCTTCTCATTATGTTTCTGTAATTGGTTTGAGTAATTGGCCAAGTAATTTTGTTAACTCACATAATTGGCTAGTTGATCTGATTAAGGCATAGTTGATTTGATTAAGGCATCGTTTTTTGAGTAAACCGGATGAGGCTGGGGACTAAGTTCAATTAAGAAGATTTAACTGGTATCTAACATAGTTTTACCATTCAAAAAATATTATGCATGTCGATTTAACTAGTTTTAAATTCTTATAGTTGTTTTTTGGTATACAATTAGACCTACATCAAATTGATTTAAATGAGTCGGTTTCAGTCCAAGATCCCTCTGAGCATAGCATGGAACAACAACAGGAGAAAACTGGCAGCGATATCAATGAGTCGGGTCATGCCGTCGCAATTCTAAACTCTAATGATAATAACTTAGTTCAAGCTGAGTCAGTACCAGAAGGAAGTCGTGAAGCAAAGAGAGGGCGTAGTGAATGGGTACATTGGGCAGAAGGTGAACATAAGTAAGTATTCAATCTATCCTTGGAAGTTATATCTTTTTTTCCTTTTCAATTCTATTTTAGGTTAAATATATTCTTTTCAATTTAAGTTATTAGATGAAGTATTAAATAGTTGTTAGATGCATAACTACGAATTTATATTTTTATTTTAGACGAATAGTCTAGTGACTAGATACTAAACTTTGGAGAAGTGGAAAATTTTACAATTAGAACCGCATGTAGATCAACGTCCTTCTAGCTACCAATTGATATGTATTTACAATAAATTAATAAAAAAACACATTTATAGCTTTTTAGTTTGTATGATGTATATAAGAAATAATTCAGTTCTAGAAAAACAAAAATGATATTTATGTTCATAGAAGCAAATTAATCTTTCTAGGTTGCATATCAACAAAGAGAAGAGAACTTGTTCAAAAACAAAGAGAAGAGACAAAAATCAATGCAAGTGTTTCCATTGTTTTGGATACATACCAAGATATAGTTACCTCATTTCTTTATTATTTCTTCTCTTATTATTTAATTGAATTTGAATATTTCGCATTCATTCTATAATTTGTGATGTATAGGTTGTTTCTGGAGGGGCTTGAAAAACATGGTAGGGGACGTTGGAAAGATATTTCAAAAGAATTTGTGGTGACAAAGACTCCAACTCAAATTGCTAGCCATGCCCAAAAATACTTTATCCACCAAAATGTGAAAGATACAGAGAAGAGGAAAAAGAGAAGAAGTATCCACGACACAACCTTAAACAAGAATGACACATCAGTCACTGCTCCTATGGAACAACATGAGAATCCTCCTCAAGACACAGCCACCTCAAGACAAATGCAGTTGGTGCTGCTCTGTCCAATCGGTTCTACACTTCCTGATATGAACAATCTTGAGAAGATGCGCAACTTGTTCAACTTGTTGGCAAAAGAACTTTGA
Protein sequence

>MS.gene070127.t1

MVIADNEISAPKSTWKKPGVDEKSVDVPVLVVGTKSWPALSVAQTPKPKNRVENVRPVASPRYQKPGPKRNVRPVTPPTHIAVPAYTFPPGSGPYSNGENPKPVSPVAAGQDLHQIDLNESVSVQDPSEHSMEQQQEKTGSDINESGHAVAILNSNDNNLVQAESVPEGSREAKRGRSEWVHWAEGEHKLFLEGLEKHGRGRWKDISKEFVVTKTPTQIASHAQKYFIHQNVKDTEKRKKRRSIHDTTLNKNDTSVTAPMEQHENPPQDTATSRQMQLVLLCPIGSTLPDMNNLEKMRNLFNLLAKEL