AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070303

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070303.t1 MTR_3g460950 77.778 126 27 1 6 131 91 215 1.88e-67 208
MS.gene070303.t1 MTR_3g060330 77.778 126 27 1 6 131 91 215 1.88e-67 208
MS.gene070303.t1 MTR_8g086000 74.603 126 31 1 6 131 91 215 4.41e-65 202
MS.gene070303.t1 MTR_8g086000 74.603 126 31 1 6 131 134 258 9.45e-65 202
MS.gene070303.t1 MTR_3g112210 73.810 126 32 1 6 131 91 215 5.58e-62 194
MS.gene070303.t1 MTR_7g078690 63.492 126 44 2 6 131 97 220 3.17e-51 166
MS.gene070303.t1 MTR_5g010030 61.905 126 46 2 6 131 100 223 8.28e-51 166
MS.gene070303.t1 MTR_8g028720 61.111 126 47 2 6 131 96 219 1.60e-50 165
MS.gene070303.t1 MTR_8g098845 60.305 131 50 2 1 131 106 234 2.00e-50 165
MS.gene070303.t1 MTR_3g060390 65.116 129 27 3 3 131 71 181 2.37e-50 163
MS.gene070303.t1 MTR_4g061320 61.111 126 47 2 6 131 99 222 2.04e-49 162
MS.gene070303.t1 MTR_4g087620 57.143 126 52 2 6 131 114 237 7.66e-49 158
MS.gene070303.t1 MTR_4g087620 57.143 126 52 2 6 131 114 237 5.73e-48 158
MS.gene070303.t1 MTR_4g061130 57.143 126 52 2 6 131 98 221 6.32e-45 150
MS.gene070303.t1 MTR_3g460980 58.333 132 35 3 3 131 71 185 1.02e-44 149
MS.gene070303.t1 MTR_3g060350 70.213 94 18 1 6 99 96 179 1.82e-37 129
MS.gene070303.t1 MTR_4g117490 55.882 102 42 2 22 120 99 200 3.41e-35 127
MS.gene070303.t1 MTR_2g021330 54.902 102 43 2 22 120 99 200 3.82e-35 125
MS.gene070303.t1 MTR_8g461040 49.153 118 56 3 6 120 85 201 4.72e-35 127
MS.gene070303.t1 MTR_4g117490 55.882 102 42 2 22 120 99 200 5.51e-35 127
MS.gene070303.t1 MTR_6g079470 50.000 118 55 3 6 120 148 264 6.11e-35 127
MS.gene070303.t1 MTR_3g089510 47.458 118 58 2 6 120 72 188 9.50e-35 126
MS.gene070303.t1 MTR_3g089510 47.458 118 58 2 6 120 84 200 1.00e-34 126
MS.gene070303.t1 MTR_2g021330 54.902 102 43 2 22 120 99 200 1.61e-34 125
MS.gene070303.t1 MTR_2g072840 46.610 118 59 2 6 120 84 200 2.48e-34 124
MS.gene070303.t1 MTR_5g091680 47.458 118 58 2 6 120 81 197 3.71e-34 123
MS.gene070303.t1 MTR_2g072840 46.610 118 59 2 6 120 84 200 5.00e-34 124
MS.gene070303.t1 MTR_5g091680 47.458 118 58 2 6 120 81 197 5.13e-34 123
MS.gene070303.t1 MTR_5g091680 47.458 118 58 2 6 120 79 195 6.02e-34 123
MS.gene070303.t1 MTR_5g091680 47.458 118 58 2 6 120 81 197 6.13e-34 123
MS.gene070303.t1 MTR_2g072840 50.980 102 47 1 22 120 9 110 1.78e-33 122
MS.gene070303.t1 MTR_8g106960 47.458 118 58 3 6 120 177 293 2.50e-33 122
MS.gene070303.t1 MTR_8g106960 47.458 118 58 3 6 120 177 293 2.56e-33 122
MS.gene070303.t1 MTR_3g460960 50.758 132 18 3 6 137 96 180 2.80e-31 112
MS.gene070303.t1 MTR_8g086010 43.089 123 36 2 8 128 192 282 4.02e-25 99.0
MS.gene070303.t1 MTR_8g080190 39.844 128 72 2 5 131 443 566 1.11e-24 98.6
MS.gene070303.t1 MTR_8g080190 39.844 128 72 2 5 131 443 566 1.13e-24 98.6
MS.gene070303.t1 MTR_8g012450 43.636 110 57 4 24 131 74 180 2.72e-24 96.3
MS.gene070303.t1 MTR_8g012450 43.119 109 59 3 24 131 74 180 9.79e-24 95.1
MS.gene070303.t1 MTR_3g085650 37.500 128 75 2 5 131 474 597 2.93e-23 94.4
MS.gene070303.t1 MTR_3g085650 37.500 128 75 2 5 131 474 597 2.96e-23 94.4
MS.gene070303.t1 MTR_3g085650 37.500 128 75 2 5 131 474 597 2.96e-23 94.4
MS.gene070303.t1 MTR_3g085650 37.500 128 75 2 5 131 474 597 2.98e-23 94.4
MS.gene070303.t1 MTR_4g103810 44.860 107 58 1 26 131 185 291 3.32e-23 93.6
MS.gene070303.t1 MTR_4g103810 44.860 107 58 1 26 131 295 401 5.09e-23 93.6
MS.gene070303.t1 MTR_1g098300 43.119 109 60 2 25 131 200 308 4.05e-22 91.3
MS.gene070303.t1 MTR_4g094430 41.964 112 62 2 23 131 74 185 9.70e-22 88.2
MS.gene070303.t1 MTR_4g007750 38.571 140 73 3 5 131 63 202 1.37e-21 88.2
MS.gene070303.t1 MTR_2g032060 38.760 129 70 3 6 131 63 185 4.42e-21 86.3
MS.gene070303.t1 MTR_4g078290 42.609 115 56 3 23 131 201 311 4.46e-21 88.2
MS.gene070303.t1 MTR_4g078290 42.609 115 56 3 23 131 201 311 4.46e-21 88.2
MS.gene070303.t1 MTR_5g008860 41.593 113 56 3 25 131 214 322 6.54e-21 87.8
MS.gene070303.t1 MTR_4g109090 36.667 120 72 3 14 131 22 139 8.35e-21 87.4
MS.gene070303.t1 MTR_4g109090 36.667 120 72 3 14 131 170 287 8.94e-21 87.4
MS.gene070303.t1 MTR_6g013030 40.367 109 63 2 25 131 184 292 1.04e-20 87.0
MS.gene070303.t1 MTR_2g090405 43.478 115 55 3 23 131 213 323 1.27e-20 87.0
MS.gene070303.t1 MTR_7g011800 38.739 111 66 2 23 131 192 302 1.87e-20 86.3
MS.gene070303.t1 MTR_6g080470 39.370 127 72 3 9 131 68 193 2.05e-20 85.9
MS.gene070303.t1 MTR_8g092290 42.202 109 61 2 25 131 215 323 3.03e-20 85.9
MS.gene070303.t1 MTR_2g085200 39.344 122 66 4 14 131 199 316 4.45e-20 85.5
MS.gene070303.t1 MTR_5g026960 40.367 109 60 4 25 131 75 180 1.20e-19 84.0
MS.gene070303.t1 MTR_3g098760 40.323 124 62 4 14 131 162 279 1.56e-19 83.6
MS.gene070303.t1 MTR_8g071230 37.615 109 63 4 25 131 75 180 9.30e-19 81.3
MS.gene070303.t1 MTR_3g054080 38.053 113 63 3 23 131 86 195 1.16e-18 80.9
MS.gene070303.t1 MTR_4g118780 36.885 122 69 4 14 131 169 286 3.03e-18 80.1
MS.gene070303.t1 MTR_3g040520 38.182 110 64 3 25 131 113 221 8.60e-18 78.6
MS.gene070303.t1 MTR_7g114300 37.097 124 66 5 14 131 172 289 9.70e-18 78.6
MS.gene070303.t1 MTR_7g114300 37.097 124 66 5 14 131 172 289 1.00e-17 78.6
MS.gene070303.t1 MTR_1g081870 40.708 113 57 3 25 131 196 304 1.03e-17 78.6
MS.gene070303.t1 MTR_7g114300 40.708 113 57 4 25 131 181 289 1.77e-17 77.8
MS.gene070303.t1 MTR_7g114300 40.708 113 57 4 25 131 181 289 1.79e-17 77.4
MS.gene070303.t1 MTR_2g020210 37.097 124 66 4 14 131 167 284 1.85e-17 77.8
MS.gene070303.t1 MTR_3g040810 37.273 110 65 3 25 131 111 219 2.72e-17 77.4
MS.gene070303.t1 MTR_1g075610 38.760 129 70 3 12 131 79 207 3.56e-17 76.3
MS.gene070303.t1 MTR_8g099770 40.367 109 63 2 25 131 164 272 4.31e-17 76.6
MS.gene070303.t1 MTR_3g096960 38.136 118 62 3 25 131 95 212 5.13e-17 76.6
MS.gene070303.t1 MTR_8g461270 40.708 113 61 3 25 131 121 233 6.14e-17 75.9
MS.gene070303.t1 MTR_4g035835 36.905 84 52 1 20 102 827 910 1.64e-14 69.3
MS.gene070303.t1 MTR_5g069000 36.905 84 52 1 20 102 831 914 2.05e-14 69.3
MS.gene070303.t1 MTR_7g076030 37.383 107 64 3 27 131 111 216 2.94e-14 68.6
MS.gene070303.t1 MTR_7g076030 37.383 107 64 3 27 131 218 323 8.01e-14 67.4
MS.gene070303.t1 MTR_7g110060 35.514 107 66 3 27 131 152 257 2.97e-13 65.9
MS.gene070303.t1 MTR_7g110060 35.514 107 66 3 27 131 152 257 3.64e-13 65.5
MS.gene070303.t1 MTR_1g086370 32.836 134 78 6 6 131 128 257 5.22e-13 65.1
MS.gene070303.t1 MTR_1g086370 34.579 107 67 3 27 131 33 138 6.49e-13 64.3
MS.gene070303.t1 MTR_1g086370 34.579 107 67 3 27 131 119 224 6.75e-13 64.7
MS.gene070303.t1 MTR_6g470940 34.524 84 54 1 20 102 945 1028 8.13e-13 64.7
MS.gene070303.t1 MTR_1g048630 32.979 94 63 0 28 121 82 175 1.06e-12 64.3
MS.gene070303.t1 MTR_8g024590 31.148 122 83 1 1 121 66 187 1.33e-12 63.9
MS.gene070303.t1 MTR_8g024600 31.148 122 83 1 1 121 66 187 1.33e-12 63.9
MS.gene070303.t1 MTR_2g083940 32.593 135 77 6 6 131 141 270 1.73e-12 63.5
MS.gene070303.t1 MTR_1g084760 33.333 111 67 4 27 131 148 257 2.61e-12 63.2
MS.gene070303.t1 MTR_3g466480 34.653 101 59 2 5 104 80 174 2.73e-12 62.8
MS.gene070303.t1 MTR_2g083940 34.579 107 67 3 27 131 111 216 3.36e-12 62.8
MS.gene070303.t1 MTR_2g005620 39.286 84 48 3 50 131 190 272 4.97e-12 62.0
MS.gene070303.t1 MTR_8g024600 35.714 98 62 1 25 121 7 104 4.98e-12 62.0
MS.gene070303.t1 MTR_1g113960 49.091 55 28 0 60 114 408 462 5.62e-12 62.0
MS.gene070303.t1 MTR_1g113960 49.091 55 28 0 60 114 408 462 5.65e-12 62.0
MS.gene070303.t1 MTR_1g103270 43.750 80 39 1 58 131 208 287 7.95e-12 61.6
MS.gene070303.t1 MTR_5g023150 49.091 55 28 0 60 114 305 359 8.60e-12 61.6
MS.gene070303.t1 MTR_5g023150 49.091 55 28 0 60 114 402 456 9.23e-12 61.6
MS.gene070303.t1 MTR_2g016340 41.096 73 41 2 25 96 85 156 1.48e-11 60.8
MS.gene070303.t1 MTR_4g114670 32.653 98 65 1 25 121 84 181 2.92e-11 60.1
MS.gene070303.t1 MTR_4g029020 33.962 106 61 2 5 107 71 170 3.44e-11 59.7
MS.gene070303.t1 MTR_5g067150 31.356 118 76 1 4 121 67 179 5.22e-11 59.3
MS.gene070303.t1 MTR_6g048250 37.838 74 45 1 24 96 91 164 6.12e-11 58.9
MS.gene070303.t1 MTR_5g067150 31.624 117 75 1 5 121 68 179 6.15e-11 58.9
MS.gene070303.t1 MTR_6g048250 37.838 74 45 1 24 96 91 164 6.32e-11 58.9
MS.gene070303.t1 MTR_5g067150 31.624 117 75 1 5 121 68 179 6.48e-11 58.9
MS.gene070303.t1 MTR_4g114670 30.702 114 77 2 25 137 84 196 7.05e-11 58.9
MS.gene070303.t1 MTR_3g099920 29.358 109 75 2 24 131 91 198 8.30e-11 58.5
MS.gene070303.t1 MTR_1g111180 35.577 104 55 4 27 123 756 854 8.77e-11 58.9
MS.gene070303.t1 MTR_1g111180 35.577 104 55 4 27 123 757 855 8.77e-11 58.9
MS.gene070303.t1 MTR_4g006970 34.409 93 53 3 5 96 79 164 9.54e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070303.t1 AT1G59580 73.016 126 33 1 6 131 91 215 2.98e-63 197
MS.gene070303.t1 AT1G59580 73.016 126 33 1 6 131 91 215 2.98e-63 197
MS.gene070303.t1 AT2G18170 73.810 126 32 1 6 131 91 215 3.65e-63 197
MS.gene070303.t1 AT1G10210 73.016 126 33 1 6 131 91 215 6.82e-62 194
MS.gene070303.t1 AT1G10210 73.016 126 33 1 6 131 91 215 6.82e-62 194
MS.gene070303.t1 AT1G10210 73.016 126 33 1 6 131 91 215 6.82e-62 194
MS.gene070303.t1 AT4G36450 69.841 126 34 2 6 131 91 212 1.72e-57 182
MS.gene070303.t1 AT1G07880 61.905 126 46 2 6 131 92 215 3.98e-52 166
MS.gene070303.t1 AT1G07880 61.905 126 46 2 6 131 92 215 3.92e-51 166
MS.gene070303.t1 AT4G01370 61.111 126 47 2 6 131 102 225 4.42e-51 166
MS.gene070303.t1 AT1G01560 60.317 126 48 2 6 131 99 222 2.55e-49 159
MS.gene070303.t1 AT1G01560 60.317 126 48 2 6 131 99 222 2.55e-49 159
MS.gene070303.t1 AT1G01560 60.317 126 48 2 6 131 99 222 5.98e-49 160
MS.gene070303.t1 AT1G01560 60.317 126 48 2 6 131 99 222 1.08e-48 160
MS.gene070303.t1 AT4G11330 59.524 126 49 2 6 131 102 225 9.37e-48 157
MS.gene070303.t1 AT2G43790 56.349 126 53 2 6 131 122 245 4.95e-47 156
MS.gene070303.t1 AT3G45640 57.937 126 51 2 6 131 97 220 3.83e-46 153
MS.gene070303.t1 AT2G46070 54.762 126 55 2 6 131 100 223 9.55e-44 146
MS.gene070303.t1 AT2G46070 54.762 126 55 2 6 131 100 223 4.53e-43 145
MS.gene070303.t1 AT2G46070 54.762 126 55 2 6 131 134 257 6.59e-43 145
MS.gene070303.t1 AT3G59790 51.587 126 59 2 6 131 119 242 1.07e-41 142
MS.gene070303.t1 AT3G14720 45.385 130 66 4 6 131 84 212 1.73e-35 127
MS.gene070303.t1 AT3G14720 45.385 130 66 4 6 131 84 212 1.73e-35 127
MS.gene070303.t1 AT2G42880 49.153 118 56 3 6 120 84 200 3.09e-35 126
MS.gene070303.t1 AT3G14720 45.385 130 66 4 6 131 84 212 6.74e-35 127
MS.gene070303.t1 AT3G18040 49.153 118 56 3 6 120 77 193 7.30e-35 126
MS.gene070303.t1 AT3G18040 49.153 118 56 3 6 120 82 198 7.65e-35 126
MS.gene070303.t1 AT2G42880 49.153 118 56 3 6 120 84 200 9.27e-35 126
MS.gene070303.t1 AT1G73670 50.000 118 55 3 6 120 149 265 1.01e-34 126
MS.gene070303.t1 AT1G18150 49.153 118 56 3 6 120 163 279 1.37e-34 126
MS.gene070303.t1 AT1G18150 49.153 118 56 3 6 120 163 279 1.37e-34 126
MS.gene070303.t1 AT1G18150 49.153 118 56 3 6 120 163 279 1.37e-34 126
MS.gene070303.t1 AT3G18040 53.922 102 44 2 22 120 9 110 1.42e-34 124
MS.gene070303.t1 AT3G18040 49.153 118 56 3 6 120 191 307 1.55e-34 126
MS.gene070303.t1 AT3G18040 49.153 118 56 3 6 120 191 307 1.73e-34 126
MS.gene070303.t1 AT5G19010 53.922 102 44 2 22 120 99 200 3.96e-34 124
MS.gene070303.t1 AT1G53510 47.458 118 58 3 6 120 84 200 4.65e-34 125
MS.gene070303.t1 AT2G01450 46.610 118 59 2 6 120 75 191 1.17e-33 122
MS.gene070303.t1 AT2G01450 46.610 118 59 2 6 120 75 191 1.17e-33 122
MS.gene070303.t1 AT2G01450 46.610 118 59 2 6 120 75 191 1.17e-33 122
MS.gene070303.t1 AT2G01450 46.610 118 59 2 6 120 75 191 1.17e-33 122
MS.gene070303.t1 AT5G63370 39.370 127 73 2 6 131 226 349 2.08e-25 99.8
MS.gene070303.t1 AT4G13020 44.954 109 57 3 24 131 74 180 2.87e-25 99.0
MS.gene070303.t1 AT4G13020 44.954 109 57 3 24 131 74 180 2.92e-25 99.4
MS.gene070303.t1 AT4G13020 44.954 109 57 3 24 131 74 180 3.03e-25 99.4
MS.gene070303.t1 AT5G63370 39.370 127 73 2 6 131 226 349 3.13e-25 99.8
MS.gene070303.t1 AT5G63370 39.370 127 73 2 6 131 226 349 3.13e-25 99.8
MS.gene070303.t1 AT4G13020 44.954 109 57 3 24 131 74 180 3.18e-25 98.6
MS.gene070303.t1 AT4G13020 44.954 109 57 3 24 131 83 189 3.28e-25 99.4
MS.gene070303.t1 AT5G63370 39.370 127 73 2 6 131 360 483 3.97e-25 99.8
MS.gene070303.t1 AT5G63370 39.370 127 73 2 6 131 360 483 3.97e-25 99.8
MS.gene070303.t1 AT4G13020 44.954 109 57 3 24 131 82 188 3.99e-25 99.0
MS.gene070303.t1 AT4G13020 44.954 109 57 3 24 131 82 188 4.02e-25 99.0
MS.gene070303.t1 AT4G13020 44.954 109 57 3 24 131 83 189 4.08e-25 98.6
MS.gene070303.t1 AT5G63370 39.370 127 73 2 6 131 360 483 4.34e-25 99.8
MS.gene070303.t1 AT5G63370 39.370 127 73 2 6 131 360 483 4.34e-25 99.8
MS.gene070303.t1 AT5G63370 39.370 127 73 2 6 131 360 483 4.34e-25 99.8
MS.gene070303.t1 AT5G63370 39.370 127 73 2 6 131 360 483 4.34e-25 99.8
MS.gene070303.t1 AT5G63370 39.370 127 73 2 6 131 360 483 4.34e-25 99.8
MS.gene070303.t1 AT1G67580 37.795 127 74 2 6 131 465 587 5.88e-22 90.9
MS.gene070303.t1 AT1G67580 37.795 127 74 2 6 131 465 587 5.88e-22 90.9
MS.gene070303.t1 AT2G38620 40.870 115 61 2 24 131 88 202 1.68e-21 87.0
MS.gene070303.t1 AT2G38620 40.870 115 61 2 24 131 88 202 1.68e-21 87.0
MS.gene070303.t1 AT1G73690 39.091 110 66 1 23 131 81 190 2.80e-21 88.2
MS.gene070303.t1 AT2G38620 40.870 115 61 2 24 131 88 202 2.97e-21 87.0
MS.gene070303.t1 AT1G71530 43.860 114 54 3 24 131 220 329 6.03e-21 87.0
MS.gene070303.t1 AT3G48750 36.567 134 76 3 1 131 58 185 9.89e-21 85.5
MS.gene070303.t1 AT1G71530 44.248 113 53 3 25 131 221 329 1.40e-20 86.7
MS.gene070303.t1 AT1G71530 44.248 113 53 3 25 131 221 329 1.54e-20 86.7
MS.gene070303.t1 AT1G71530 44.248 113 53 3 25 131 221 329 1.84e-20 86.7
MS.gene070303.t1 AT1G54610 40.164 122 65 4 14 131 183 300 4.76e-20 85.1
MS.gene070303.t1 AT1G54610 40.164 122 65 4 14 131 183 300 4.76e-20 85.1
MS.gene070303.t1 AT1G54610 40.164 122 65 4 14 131 183 300 4.76e-20 85.1
MS.gene070303.t1 AT1G18670 36.364 121 72 3 14 131 196 314 4.99e-20 85.1
MS.gene070303.t1 AT1G18670 36.364 121 72 3 14 131 196 314 5.03e-20 85.1
MS.gene070303.t1 AT1G18670 36.364 121 72 3 14 131 196 314 5.04e-20 85.1
MS.gene070303.t1 AT5G44290 44.248 113 53 3 25 131 211 319 7.31e-20 84.7
MS.gene070303.t1 AT5G44290 44.248 113 53 3 25 131 211 319 7.80e-20 84.7
MS.gene070303.t1 AT5G44290 44.248 113 53 3 25 131 211 319 7.83e-20 84.7
MS.gene070303.t1 AT5G44290 44.248 113 53 3 25 131 211 319 7.83e-20 84.7
MS.gene070303.t1 AT5G44290 44.248 113 53 3 25 131 211 319 7.83e-20 84.7
MS.gene070303.t1 AT5G44290 44.248 113 53 3 25 131 211 319 7.83e-20 84.7
MS.gene070303.t1 AT5G44290 44.248 113 53 3 25 131 211 319 7.83e-20 84.7
MS.gene070303.t1 AT1G18040 38.182 110 67 1 23 131 82 191 9.72e-20 84.0
MS.gene070303.t1 AT5G50860 38.843 121 68 4 14 131 179 296 1.06e-19 84.3
MS.gene070303.t1 AT5G50860 38.843 121 68 4 14 131 179 296 1.13e-19 84.3
MS.gene070303.t1 AT4G22940 37.121 132 70 4 6 131 162 286 1.20e-19 84.0
MS.gene070303.t1 AT4G22940 37.121 132 70 4 6 131 162 286 1.43e-19 84.0
MS.gene070303.t1 AT1G53050 39.516 124 63 4 14 131 199 316 1.64e-19 84.0
MS.gene070303.t1 AT1G53050 39.516 124 63 4 14 131 199 316 1.64e-19 84.0
MS.gene070303.t1 AT1G74330 35.246 122 73 3 14 131 186 305 2.55e-19 83.2
MS.gene070303.t1 AT1G74330 35.246 122 73 3 14 131 186 305 2.58e-19 83.2
MS.gene070303.t1 AT1G74330 35.246 122 73 3 14 131 186 305 2.58e-19 83.2
MS.gene070303.t1 AT4G10010 41.284 109 62 2 25 131 50 158 3.41e-19 82.8
MS.gene070303.t1 AT4G10010 41.284 109 62 2 25 131 230 338 3.84e-19 82.8
MS.gene070303.t1 AT1G03740 40.708 113 57 3 25 131 287 395 5.08e-19 82.4
MS.gene070303.t1 AT1G03740 40.708 113 57 3 25 131 287 395 5.12e-19 82.4
MS.gene070303.t1 AT1G33770 42.478 113 55 3 25 131 215 323 7.15e-19 82.0
MS.gene070303.t1 AT2G38620 36.154 130 80 2 3 131 13 140 8.43e-19 79.7
MS.gene070303.t1 AT3G54180 40.000 115 62 2 24 131 86 200 9.16e-19 80.5
MS.gene070303.t1 AT4G19110 38.532 109 62 4 25 131 19 124 1.11e-18 80.9
MS.gene070303.t1 AT1G66750 38.889 108 65 1 25 131 85 192 1.65e-18 80.1
MS.gene070303.t1 AT4G19110 38.532 109 62 4 25 131 75 180 2.78e-18 80.1
MS.gene070303.t1 AT4G19110 38.532 109 62 4 25 131 75 180 2.79e-18 80.1
MS.gene070303.t1 AT5G39420 38.739 111 62 3 25 131 179 287 3.59e-18 80.1
MS.gene070303.t1 AT3G01085 37.615 109 66 2 25 131 189 297 5.28e-18 79.3
MS.gene070303.t1 AT3G01085 37.615 109 66 2 25 131 189 297 5.28e-18 79.3
MS.gene070303.t1 AT3G01085 37.615 109 66 2 25 131 189 297 5.28e-18 79.3
MS.gene070303.t1 AT1G76540 37.209 129 72 3 12 131 75 203 7.74e-18 78.2
MS.gene070303.t1 AT5G63610 40.179 112 61 2 26 131 106 217 1.16e-17 78.2
MS.gene070303.t1 AT5G64960 37.615 109 66 2 25 131 61 169 3.87e-17 77.0
MS.gene070303.t1 AT5G10270 37.615 109 66 2 25 131 114 222 4.04e-17 77.0
MS.gene070303.t1 AT5G64960 37.615 109 66 2 25 131 114 222 4.06e-17 77.0
MS.gene070303.t1 AT1G20930 36.434 129 73 3 12 131 77 205 4.96e-17 75.9
MS.gene070303.t1 AT5G45430 37.037 108 65 3 25 131 75 180 7.13e-17 76.3
MS.gene070303.t1 AT5G45430 37.037 108 65 3 25 131 75 180 7.14e-17 76.3
MS.gene070303.t1 AT3G05050 37.615 109 66 2 25 131 212 320 3.27e-16 74.3
MS.gene070303.t1 AT3G05050 37.615 109 66 2 25 131 212 320 3.27e-16 74.3
MS.gene070303.t1 AT3G05050 37.615 109 66 2 25 131 212 320 3.27e-16 74.3
MS.gene070303.t1 AT5G62310 39.286 84 50 1 20 102 822 905 6.03e-16 73.6
MS.gene070303.t1 AT1G09600 40.541 111 60 3 25 131 237 345 4.44e-14 68.2
MS.gene070303.t1 AT1G45160 37.931 87 53 1 20 105 738 824 8.74e-14 67.4
MS.gene070303.t1 AT1G45160 37.931 87 53 1 20 105 738 824 9.80e-14 67.4
MS.gene070303.t1 AT1G57700 40.708 113 57 4 25 131 220 328 2.00e-13 66.2
MS.gene070303.t1 AT3G17850 35.714 84 53 1 20 102 950 1033 3.12e-13 65.9
MS.gene070303.t1 AT2G30980 34.862 109 64 4 27 131 149 254 4.09e-13 65.5
MS.gene070303.t1 AT1G57870 41.667 84 46 3 50 131 182 264 5.00e-13 65.1
MS.gene070303.t1 AT1G57870 41.667 84 46 3 50 131 182 264 5.00e-13 65.1
MS.gene070303.t1 AT1G57870 41.667 84 46 3 50 131 182 264 5.00e-13 65.1
MS.gene070303.t1 AT1G50240 32.979 94 63 0 28 121 81 174 5.91e-13 65.1
MS.gene070303.t1 AT1G50240 32.979 94 63 0 28 121 81 174 5.91e-13 65.1
MS.gene070303.t1 AT1G57870 41.667 84 46 3 50 131 182 264 6.37e-13 64.7
MS.gene070303.t1 AT5G10930 37.363 91 51 1 6 96 73 157 6.57e-13 64.7
MS.gene070303.t1 AT1G09840 39.286 84 48 3 50 131 183 265 2.10e-12 63.2
MS.gene070303.t1 AT1G09840 39.286 84 48 3 50 131 183 265 2.10e-12 63.2
MS.gene070303.t1 AT1G09840 39.286 84 48 3 50 131 183 265 2.10e-12 63.2
MS.gene070303.t1 AT1G09840 39.286 84 48 3 50 131 183 265 2.10e-12 63.2
MS.gene070303.t1 AT1G09840 39.286 84 48 3 50 131 183 265 2.10e-12 63.2
MS.gene070303.t1 AT1G09840 39.286 84 48 3 50 131 183 265 2.10e-12 63.2
MS.gene070303.t1 AT1G09840 39.286 84 48 3 50 131 183 265 2.10e-12 63.2
MS.gene070303.t1 AT1G09840 39.286 84 48 3 50 131 183 265 2.10e-12 63.2
MS.gene070303.t1 AT1G09840 39.286 84 48 3 50 131 183 265 2.10e-12 63.2
MS.gene070303.t1 AT1G09840 39.286 84 48 3 50 131 183 265 2.10e-12 63.2
MS.gene070303.t1 AT1G06390 33.645 107 68 3 27 131 147 252 2.20e-12 63.2
MS.gene070303.t1 AT1G06390 33.645 107 68 3 27 131 147 252 2.20e-12 63.2
MS.gene070303.t1 AT2G30360 34.951 103 59 3 1 101 77 173 2.71e-12 63.2
MS.gene070303.t1 AT1G48490 33.333 84 55 1 20 102 896 979 2.82e-12 63.2
MS.gene070303.t1 AT1G48490 33.333 84 55 1 20 102 896 979 2.82e-12 63.2
MS.gene070303.t1 AT1G48490 33.333 84 55 1 20 102 896 979 2.82e-12 63.2
MS.gene070303.t1 AT1G48490 33.333 84 55 1 20 102 897 980 2.82e-12 63.2
MS.gene070303.t1 AT2G38490 33.333 135 81 4 5 137 87 214 2.83e-12 62.8
MS.gene070303.t1 AT1G30270 38.961 77 46 1 25 100 7 83 3.29e-12 62.8
MS.gene070303.t1 AT1G09840 34.579 107 67 3 27 131 33 138 3.55e-12 62.4
MS.gene070303.t1 AT4G00720 37.500 96 57 3 38 131 226 320 3.77e-12 62.8
MS.gene070303.t1 AT5G26751 33.333 111 67 4 27 131 142 251 3.97e-12 62.4
MS.gene070303.t1 AT3G05840 33.333 111 67 4 27 131 146 255 4.48e-12 62.4
MS.gene070303.t1 AT3G05840 33.333 111 67 4 27 131 146 255 4.48e-12 62.4
MS.gene070303.t1 AT1G13350 36.538 104 54 4 27 123 523 621 6.50e-12 62.0
MS.gene070303.t1 AT1G13350 36.538 104 54 4 27 123 550 648 6.98e-12 62.0
MS.gene070303.t1 AT1G13350 36.538 104 54 4 27 123 596 694 8.77e-12 61.6
MS.gene070303.t1 AT1G30270 36.458 96 54 2 6 100 91 180 1.06e-11 61.2
MS.gene070303.t1 AT1G30270 36.458 96 54 2 6 100 91 180 1.06e-11 61.2
MS.gene070303.t1 AT5G25110 34.409 93 51 3 6 96 103 187 1.10e-11 61.2
MS.gene070303.t1 AT3G01090 37.838 74 45 1 24 96 114 187 1.22e-11 61.2
MS.gene070303.t1 AT3G01090 37.838 74 45 1 24 96 91 164 1.28e-11 61.2
MS.gene070303.t1 AT3G01090 37.838 74 45 1 24 96 91 164 1.28e-11 61.2
MS.gene070303.t1 AT3G29160 37.838 74 45 1 24 96 92 165 1.59e-11 60.8
MS.gene070303.t1 AT2G25090 33.696 92 54 2 6 96 76 161 2.00e-11 60.5
MS.gene070303.t1 AT3G29160 37.838 74 45 1 24 96 92 165 2.26e-11 60.5
MS.gene070303.t1 AT3G29160 37.838 74 45 1 24 96 92 165 2.26e-11 60.5
MS.gene070303.t1 AT5G14640 31.776 107 70 3 27 131 151 256 2.93e-11 60.1
MS.gene070303.t1 AT5G14640 31.776 107 70 3 27 131 151 256 2.93e-11 60.1
MS.gene070303.t1 AT4G24400 34.783 92 59 1 46 137 104 194 4.42e-11 59.7
MS.gene070303.t1 AT4G24400 34.783 92 59 1 46 137 104 194 4.50e-11 59.7
MS.gene070303.t1 AT4G35780 47.273 55 29 0 60 114 399 453 4.53e-11 59.7
MS.gene070303.t1 AT4G38470 45.614 57 31 0 58 114 395 451 4.71e-11 59.3
MS.gene070303.t1 AT4G38470 45.614 57 31 0 58 114 395 451 4.78e-11 59.3
MS.gene070303.t1 AT4G38470 45.614 57 31 0 58 114 395 451 4.81e-11 59.3
MS.gene070303.t1 AT1G18350 37.838 74 44 1 24 97 117 188 6.59e-11 58.9
MS.gene070303.t1 AT1G32320 38.667 75 45 1 24 97 114 188 6.61e-11 58.9
MS.gene070303.t1 AT5G45820 35.870 92 53 1 5 96 71 156 7.42e-11 58.9
MS.gene070303.t1 AT1G73500 36.486 74 45 1 24 97 119 190 8.77e-11 58.5
MS.gene070303.t1 AT1G78290 36.190 105 63 3 35 137 84 186 9.45e-11 58.5
MS.gene070303.t1 AT1G78290 36.190 105 63 3 35 137 84 186 9.45e-11 58.5
MS.gene070303.t1 AT1G30640 32.099 81 51 1 22 98 94 174 9.67e-11 58.5
MS.gene070303.t1 AT1G30640 32.099 81 51 1 22 98 187 267 9.99e-11 58.5
MS.gene070303.t1 AT1G30640 32.099 81 51 1 22 98 187 267 9.99e-11 58.5

Find 22 sgRNAs with CRISPR-Local

Find 36 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACCATTGCCGATATTTCT+TGG 0.281243 3.3:-48666309 MS.gene070303:CDS
CGGCAATGGTCATTGGAAAT+TGG 0.300842 3.3:+48666320 None:intergenic
TGAGCAAATTTGCAGGCTTC+AGG 0.314387 3.3:+48665846 None:intergenic
GCTCGGACCAATCAAGTTAA+TGG 0.324476 3.3:-48665782 MS.gene070303:CDS
AATCTCGCCATTAACTTGAT+TGG 0.360614 3.3:+48665775 None:intergenic
CTATTAGCATGTGACAATTA+CGG 0.396118 3.3:-48665707 MS.gene070303:CDS
TCATTAATGAGCAAATTTGC+AGG 0.410176 3.3:+48665839 None:intergenic
GAAATATCGGCAATGGTCAT+TGG 0.411829 3.3:+48666313 None:intergenic
CTACTTTGTCTATGAACTCA+TGG 0.455179 3.3:-48666378 MS.gene070303:CDS
GTACCTGCGCCAAGAAATAT+CGG 0.461903 3.3:+48666300 None:intergenic
TAAAGTTGCTTAGACATGTC+CGG 0.526853 3.3:-48666467 None:intergenic
TTGCCGATATTTCTTGGCGC+AGG 0.547464 3.3:-48666303 MS.gene070303:intron
CAAAGTGATCACATATGTGC+CGG 0.561565 3.3:+48666448 None:intergenic
GCAAGAATGCATCCCACGGA+TGG 0.565016 3.3:+48665679 None:intergenic
TATGTGACTTCGGACTTGCT+CGG 0.583313 3.3:-48665799 MS.gene070303:CDS
GCGCCAAGAAATATCGGCAA+TGG 0.597426 3.3:+48666306 None:intergenic
CAGAGTATATTGTCACGCGT+TGG 0.604813 3.3:-48665748 MS.gene070303:CDS
CTCAGCAAGAATGCATCCCA+CGG 0.610293 3.3:+48665675 None:intergenic
GTCATTGGAAATTGGCTCAG+AGG 0.613015 3.3:+48666328 None:intergenic
GATTTGAAGATATGTGACTT+CGG 0.636250 3.3:-48665809 MS.gene070303:CDS
AATTACGGAACATCCATCCG+TGG 0.663559 3.3:-48665692 MS.gene070303:CDS
ATTACGGAACATCCATCCGT+GGG 0.671330 3.3:-48665691 MS.gene070303:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATATTTTCAGTTATTTCTAT+TGG - chr3.3:48665866-48665885 MS.gene070303:CDS 15.0%
!! AGAAAATTATAGCTCATAAT+AGG + chr3.3:48666072-48666091 None:intergenic 20.0%
!! ATTACTATTCCTATTATCAA+TGG + chr3.3:48666140-48666159 None:intergenic 20.0%
!! TAGAAATAACTGAAAATATG+AGG + chr3.3:48665865-48665884 None:intergenic 20.0%
! AAACTCAAATTAAAGGTAGT+TGG - chr3.3:48665906-48665925 MS.gene070303:intron 25.0%
! AACTAGAGGATGAAAATTAT+TGG - chr3.3:48665969-48665988 MS.gene070303:intron 25.0%
! TCAGTCAAAACTCAAATTAA+AGG - chr3.3:48665899-48665918 MS.gene070303:CDS 25.0%
! TTATAGAATGTATGAACTAG+AGG - chr3.3:48665955-48665974 MS.gene070303:intron 25.0%
!!! CCAAAAAACAACTTGTTTTA+AGG - chr3.3:48665723-48665742 MS.gene070303:CDS 25.0%
!!! CCTTAAAACAAGTTGTTTTT+TGG + chr3.3:48665726-48665745 None:intergenic 25.0%
CTATTAGCATGTGACAATTA+CGG - chr3.3:48666421-48666440 MS.gene070303:CDS 30.0%
GATTTGAAGATATGTGACTT+CGG - chr3.3:48666319-48666338 MS.gene070303:CDS 30.0%
TCACATTAATTTCCCAACTT+GGG + chr3.3:48665995-48666014 None:intergenic 30.0%
TCATTAATGAGCAAATTTGC+AGG + chr3.3:48666292-48666311 None:intergenic 30.0%
! TGTTGATCACCATTGATAAT+AGG - chr3.3:48666128-48666147 MS.gene070303:intron 30.0%
!!! CTCTTATTTTAGTTGCTTGA+AGG - chr3.3:48666220-48666239 MS.gene070303:intron 30.0%
CTACTTTGTCTATGAACTCA+TGG - chr3.3:48665750-48665769 MS.gene070303:CDS 35.0%
CTCACATTAATTTCCCAACT+TGG + chr3.3:48665996-48666015 None:intergenic 35.0%
GAAAATTATTGGCCCAAGTT+GGG - chr3.3:48665980-48665999 MS.gene070303:intron 35.0%
TGAAAATTATTGGCCCAAGT+TGG - chr3.3:48665979-48665998 MS.gene070303:intron 35.0%
! AATCTCGCCATTAACTTGAT+TGG + chr3.3:48666356-48666375 None:intergenic 35.0%
CAAAGTGATCACATATGTGC+CGG + chr3.3:48665683-48665702 None:intergenic 40.0%
GAAATATCGGCAATGGTCAT+TGG + chr3.3:48665818-48665837 None:intergenic 40.0%
TGACCATTGCCGATATTTCT+TGG - chr3.3:48665819-48665838 MS.gene070303:CDS 40.0%
AATTACGGAACATCCATCCG+TGG - chr3.3:48666436-48666455 MS.gene070303:CDS 45.0%
ATTACGGAACATCCATCCGT+GGG - chr3.3:48666437-48666456 MS.gene070303:CDS 45.0%
CAGAGTATATTGTCACGCGT+TGG - chr3.3:48666380-48666399 MS.gene070303:CDS 45.0%
CGGCAATGGTCATTGGAAAT+TGG + chr3.3:48665811-48665830 None:intergenic 45.0%
GCTCGGACCAATCAAGTTAA+TGG - chr3.3:48666346-48666365 MS.gene070303:CDS 45.0%
GTACCTGCGCCAAGAAATAT+CGG + chr3.3:48665831-48665850 None:intergenic 45.0%
TGAGCAAATTTGCAGGCTTC+AGG + chr3.3:48666285-48666304 None:intergenic 45.0%
! GTCATTGGAAATTGGCTCAG+AGG + chr3.3:48665803-48665822 None:intergenic 45.0%
! TATGTGACTTCGGACTTGCT+CGG - chr3.3:48666329-48666348 MS.gene070303:CDS 45.0%
GCGCCAAGAAATATCGGCAA+TGG + chr3.3:48665825-48665844 None:intergenic 50.0%
TTGCCGATATTTCTTGGCGC+AGG - chr3.3:48665825-48665844 MS.gene070303:CDS 50.0%
GCAAGAATGCATCCCACGGA+TGG + chr3.3:48666452-48666471 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr3.3 gene 48665676 48666474 48665676 ID=MS.gene070303
chr3.3 mRNA 48665676 48666474 48665676 ID=MS.gene070303.t1;Parent=MS.gene070303
chr3.3 exon 48666304 48666474 48666304 ID=MS.gene070303.t1.exon1;Parent=MS.gene070303.t1
chr3.3 CDS 48666304 48666474 48666304 ID=cds.MS.gene070303.t1;Parent=MS.gene070303.t1
chr3.3 exon 48665676 48665918 48665676 ID=MS.gene070303.t1.exon2;Parent=MS.gene070303.t1
chr3.3 CDS 48665676 48665918 48665676 ID=cds.MS.gene070303.t1;Parent=MS.gene070303.t1
Gene Sequence

>MS.gene070303

ATGTCCGGCACATATGTGATCACTTTGAAAGATGTTATGATACTAGACCAAAAAACAACTTGTTTTAAGGATGTCTACTTTGTCTATGAACTCATGGATACTGATCTTCATCATGTTATCAAGTCCTCTGAGCCAATTTCCAATGACCATTGCCGATATTTCTTGGCGCAGGTACTAATTTCAATTCCTCATATTTTCAGTTATTTCTATTGGCTTTACATTTTCAGTCAAAACTCAAATTAAAGGTAGTTGGTCTTGAATGAGTATACACTGTCATTCTTATAGAATGTATGAACTAGAGGATGAAAATTATTGGCCCAAGTTGGGAAATTAATGTGAGCTTAACATTGATTATTGATTAAAATAAAGTTCTTGTGAAAAGATTCAAAAGAGCCTATTATGAGCTATAATTTTCTTGTCTGCATTTTTTTAATATATGTTTTTGAGTATATTGTTGATCACCATTGATAATAGGAATAGTAATATACTAGTACAAAGCAATTCACTTAGTTTACGTATCTTTCTGATTGTTACTAATCATAAACTCTTATTTTAGTTGCTTGAAGGTCTTAATCATCTTCATTCTGCAAATATTCTTCATCGTGACCTGAAGCCTGCAAATTTGCTCATTAATGAAAATTGTGATTTGAAGATATGTGACTTCGGACTTGCTCGGACCAATCAAGTTAATGGCGAGATTATGACAGAGTATATTGTCACGCGTTGGTATCATGCTCTAGAGCTTCTATTAGCATGTGACAATTACGGAACATCCATCCGTGGGATGCATTCTTGCTGA
Protein sequence

>MS.gene070303.t1

MSGTYVITLKDVMILDQKTTCFKDVYFVYELMDTDLHHVIKSSEPISNDHCRYFLAQLLEGLNHLHSANILHRDLKPANLLINENCDLKICDFGLARTNQVNGEIMTEYIVTRWYHALELLLACDNYGTSIRGMHSC