Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070434.t1 | RHN66404.1 | 86.6 | 187 | 25 | 0 | 1 | 187 | 1 | 187 | 9.30E-73 | 283.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070434.t1 | Q8GTS1 | 46.1 | 167 | 81 | 4 | 4 | 162 | 10 | 175 | 2.1e-25 | 117.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070434.t1 | A0A396IPX9 | 86.6 | 187 | 25 | 0 | 1 | 187 | 1 | 187 | 6.7e-73 | 283.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene070434.t1 | TF | bZIP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070434.t1 | MTR_4g073100 | 44.828 | 203 | 86 | 5 | 1 | 183 | 1 | 197 | 3.71e-51 | 166 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene070434.t1 | AT3G51960 | 50.000 | 168 | 74 | 4 | 4 | 162 | 10 | 176 | 6.85e-48 | 156 |
| MS.gene070434.t1 | AT3G51960 | 50.299 | 167 | 74 | 4 | 4 | 162 | 10 | 175 | 9.52e-48 | 155 |
| MS.gene070434.t1 | AT3G51960 | 56.250 | 128 | 50 | 1 | 41 | 162 | 10 | 137 | 1.30e-47 | 154 |
| MS.gene070434.t1 | AT2G16770 | 52.288 | 153 | 60 | 5 | 29 | 169 | 18 | 169 | 2.22e-47 | 155 |
| MS.gene070434.t1 | AT2G16770 | 52.288 | 153 | 60 | 5 | 29 | 169 | 18 | 169 | 2.22e-47 | 155 |
| MS.gene070434.t1 | AT4G35040 | 48.087 | 183 | 71 | 8 | 1 | 165 | 1 | 177 | 3.08e-45 | 150 |
| MS.gene070434.t1 | AT4G35040 | 48.087 | 183 | 71 | 8 | 1 | 165 | 1 | 177 | 3.08e-45 | 150 |
| MS.gene070434.t1 | AT4G35040 | 48.087 | 183 | 71 | 8 | 1 | 165 | 1 | 177 | 3.08e-45 | 150 |
| MS.gene070434.t1 | AT4G35040 | 48.087 | 183 | 71 | 8 | 1 | 165 | 1 | 177 | 3.08e-45 | 150 |
Find 22 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAGGCAGAAACAGCTTATT+TGG | 0.185081 | 3.3:+25540629 | MS.gene070434:CDS |
| TGAAAATTGCTACATGATTC+TGG | 0.201465 | 3.3:-25540384 | None:intergenic |
| GTCAAGGTTAAGGAACATTT+TGG | 0.239454 | 3.3:+25540734 | MS.gene070434:CDS |
| GGAAAAGTTGACAATGAATT+AGG | 0.247864 | 3.3:+25540768 | MS.gene070434:CDS |
| AAAGTATAGGGAGAAGAAAA+AGG | 0.319200 | 3.3:+25540611 | MS.gene070434:CDS |
| GTAGTTTCCTTAAAAGTTGT+TGG | 0.338670 | 3.3:-25540683 | None:intergenic |
| AAGCTGAATTGTCAAGGTTA+AGG | 0.395629 | 3.3:+25540724 | MS.gene070434:CDS |
| ACACAAATTCAAGGCCAAAA+AGG | 0.410549 | 3.3:+25540562 | MS.gene070434:CDS |
| ACATGCAGGATCATCATCTA+AGG | 0.417339 | 3.3:+25540830 | MS.gene070434:CDS |
| GTCCATTAGTATTACTCATG+TGG | 0.478158 | 3.3:-25540876 | None:intergenic |
| AAGAAAGAAAATATGGAAGA+TGG | 0.479572 | 3.3:+25540312 | None:intergenic |
| CTGAAGATGACACAAATTCA+AGG | 0.502147 | 3.3:+25540553 | MS.gene070434:CDS |
| CCATGTTTGCATCCACATGC+AGG | 0.525489 | 3.3:+25540816 | MS.gene070434:CDS |
| TTTAAGGAAACTACAAGGGC+AGG | 0.534031 | 3.3:+25540692 | MS.gene070434:CDS |
| TACTTGAAGCTGAATTGTCA+AGG | 0.544162 | 3.3:+25540718 | MS.gene070434:CDS |
| CCTGCATGTGGATGCAAACA+TGG | 0.553344 | 3.3:-25540816 | None:intergenic |
| TGTGTGTGATAAGCATCATC+AGG | 0.565504 | 3.3:-25540495 | None:intergenic |
| TGATAAGCATCATCAGGACC+AGG | 0.614933 | 3.3:-25540489 | None:intergenic |
| ACTGTGTTCATAGATGTTGA+AGG | 0.626655 | 3.3:-25540408 | None:intergenic |
| TTAGATGATGATCCTGCATG+TGG | 0.661059 | 3.3:-25540828 | None:intergenic |
| ACTCACACATGTAATCCACC+TGG | 0.668836 | 3.3:+25540471 | MS.gene070434:CDS |
| TAAGCATCATCAGGACCAGG+TGG | 0.696090 | 3.3:-25540486 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AGAAGCTGTTAAAAAGTATA+GGG | + | chr3.3:25540599-25540618 | MS.gene070434:CDS | 25.0% |
| !!! | AACTTTTAAGGAAACTACAA+GGG | + | chr3.3:25540688-25540707 | MS.gene070434:CDS | 25.0% |
| AAAGTATAGGGAGAAGAAAA+AGG | + | chr3.3:25540611-25540630 | MS.gene070434:CDS | 30.0% | |
| GGAAAAGTTGACAATGAATT+AGG | + | chr3.3:25540768-25540787 | MS.gene070434:CDS | 30.0% | |
| GTAGTTTCCTTAAAAGTTGT+TGG | - | chr3.3:25540686-25540705 | None:intergenic | 30.0% | |
| TGAAAATTGCTACATGATTC+TGG | - | chr3.3:25540387-25540406 | None:intergenic | 30.0% | |
| ! | GAGAAGCTGTTAAAAAGTAT+AGG | + | chr3.3:25540598-25540617 | MS.gene070434:CDS | 30.0% |
| ! | TTGTGAACCAACAACTTTTA+AGG | + | chr3.3:25540676-25540695 | MS.gene070434:CDS | 30.0% |
| !! | AACATTTTGGTACAGCTTAA+GGG | + | chr3.3:25540747-25540766 | MS.gene070434:CDS | 30.0% |
| !! | CAACTTTTAAGGAAACTACA+AGG | + | chr3.3:25540687-25540706 | MS.gene070434:CDS | 30.0% |
| AAAGGCAGAAACAGCTTATT+TGG | + | chr3.3:25540629-25540648 | MS.gene070434:CDS | 35.0% | |
| AAGCTGAATTGTCAAGGTTA+AGG | + | chr3.3:25540724-25540743 | MS.gene070434:CDS | 35.0% | |
| ACACAAATTCAAGGCCAAAA+AGG | + | chr3.3:25540562-25540581 | MS.gene070434:CDS | 35.0% | |
| ACTGTGTTCATAGATGTTGA+AGG | - | chr3.3:25540411-25540430 | None:intergenic | 35.0% | |
| CTGAAGATGACACAAATTCA+AGG | + | chr3.3:25540553-25540572 | MS.gene070434:CDS | 35.0% | |
| TACTTGAAGCTGAATTGTCA+AGG | + | chr3.3:25540718-25540737 | MS.gene070434:CDS | 35.0% | |
| ! | CATGGCAGATTTTTCTCAAA+AGG | - | chr3.3:25540801-25540820 | None:intergenic | 35.0% |
| ! | GTCAAGGTTAAGGAACATTT+TGG | + | chr3.3:25540734-25540753 | MS.gene070434:CDS | 35.0% |
| !! | AGTGTGAATGCATGTTTTTG+TGG | - | chr3.3:25540454-25540473 | None:intergenic | 35.0% |
| !! | GAACATTTTGGTACAGCTTA+AGG | + | chr3.3:25540746-25540765 | MS.gene070434:CDS | 35.0% |
| ACATGCAGGATCATCATCTA+AGG | + | chr3.3:25540830-25540849 | MS.gene070434:CDS | 40.0% | |
| TGTGTGTGATAAGCATCATC+AGG | - | chr3.3:25540498-25540517 | None:intergenic | 40.0% | |
| TTAGATGATGATCCTGCATG+TGG | - | chr3.3:25540831-25540850 | None:intergenic | 40.0% | |
| TTTAAGGAAACTACAAGGGC+AGG | + | chr3.3:25540692-25540711 | MS.gene070434:CDS | 40.0% | |
| ! | CTATTGCCAGAAGTCCTTTT+TGG | - | chr3.3:25540579-25540598 | None:intergenic | 40.0% |
| ACTCACACATGTAATCCACC+TGG | + | chr3.3:25540471-25540490 | MS.gene070434:CDS | 45.0% | |
| TCAAGGCCAAAAAGGACTTC+TGG | + | chr3.3:25540570-25540589 | MS.gene070434:CDS | 45.0% | |
| TGATAAGCATCATCAGGACC+AGG | - | chr3.3:25540492-25540511 | None:intergenic | 45.0% | |
| CCATGTTTGCATCCACATGC+AGG | + | chr3.3:25540816-25540835 | MS.gene070434:CDS | 50.0% | |
| CCTGCATGTGGATGCAAACA+TGG | - | chr3.3:25540819-25540838 | None:intergenic | 50.0% | |
| TAAGCATCATCAGGACCAGG+TGG | - | chr3.3:25540489-25540508 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 25540324 | 25540887 | 25540324 | ID=MS.gene070434 |
| chr3.3 | mRNA | 25540324 | 25540887 | 25540324 | ID=MS.gene070434.t1;Parent=MS.gene070434 |
| chr3.3 | exon | 25540324 | 25540887 | 25540324 | ID=MS.gene070434.t1.exon1;Parent=MS.gene070434.t1 |
| chr3.3 | CDS | 25540324 | 25540887 | 25540324 | ID=cds.MS.gene070434.t1;Parent=MS.gene070434.t1 |
| Gene Sequence |
| Protein sequence |
