AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070516


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070516.t1 MTR_5g085390 94.468 235 10 3 1 233 1 234 1.65e-138 388
MS.gene070516.t1 MTR_6g011200 56.967 244 84 6 1 233 1 234 6.08e-81 243
MS.gene070516.t1 MTR_6g011250 63.121 141 50 1 1 139 1 141 3.18e-59 185
MS.gene070516.t1 MTR_6g011230 47.257 237 81 11 1 228 1 202 6.72e-55 174
MS.gene070516.t1 MTR_4g099230 63.910 133 43 1 10 142 3 130 1.11e-54 172
MS.gene070516.t1 MTR_6g004550 55.556 126 50 2 37 159 1 123 1.90e-37 128
MS.gene070516.t1 MTR_4g107450 54.717 106 47 1 8 113 9 113 1.06e-31 117
MS.gene070516.t1 MTR_8g017090 53.333 105 48 1 7 111 10 113 1.20e-29 112
MS.gene070516.t1 MTR_3g073690 45.098 102 55 1 10 111 10 110 3.73e-28 106
MS.gene070516.t1 MTR_7g033800 34.545 165 80 4 7 159 9 157 2.34e-27 103
MS.gene070516.t1 MTR_3g452660 44.762 105 57 1 7 111 5 108 4.77e-27 105
MS.gene070516.t1 MTR_4g083680 32.164 171 97 5 10 178 16 169 4.77e-27 102
MS.gene070516.t1 MTR_5g017950 43.810 105 58 1 7 111 6 109 9.70e-27 105
MS.gene070516.t1 MTR_8g040900 47.126 87 45 1 3 89 1 86 2.47e-26 102
MS.gene070516.t1 MTR_4g105170 42.056 107 60 2 7 112 9 114 1.08e-25 99.4
MS.gene070516.t1 MTR_6g005080 43.878 98 54 1 7 104 8 104 2.03e-25 97.4
MS.gene070516.t1 MTR_6g005070 43.878 98 54 1 7 104 7 103 2.50e-25 97.4
MS.gene070516.t1 MTR_3g077240 42.857 98 55 1 7 104 8 104 4.43e-25 97.8
MS.gene070516.t1 MTR_2g100020 45.283 106 57 1 8 113 18 122 5.27e-25 99.8
MS.gene070516.t1 MTR_5g083960 36.449 107 64 2 1 107 34 136 8.10e-25 97.4
MS.gene070516.t1 MTR_5g075020 38.938 113 64 3 1 112 38 146 1.11e-24 97.8
MS.gene070516.t1 MTR_5g075020 38.938 113 64 3 1 112 42 150 1.44e-24 97.8
MS.gene070516.t1 MTR_3g031660 36.441 118 74 1 10 127 8 124 3.09e-24 95.5
MS.gene070516.t1 MTR_5g093010 32.639 144 88 4 10 149 6 144 4.38e-24 94.0
MS.gene070516.t1 MTR_2g068760 42.105 95 50 2 10 104 14 103 8.67e-24 93.6
MS.gene070516.t1 MTR_2g093310 48.750 80 40 1 10 89 34 112 1.68e-23 94.0
MS.gene070516.t1 MTR_7g028905 37.864 103 63 1 10 112 6 107 7.61e-23 90.1
MS.gene070516.t1 MTR_3g094690 38.182 110 60 3 1 110 42 143 8.27e-23 93.2
MS.gene070516.t1 MTR_5g083230 42.169 83 47 1 7 89 4 85 2.22e-22 90.1
MS.gene070516.t1 MTR_5g083010 38.053 113 61 3 5 113 7 114 2.71e-22 90.1
MS.gene070516.t1 MTR_4g060950 43.158 95 52 2 10 103 25 118 3.83e-22 90.1
MS.gene070516.t1 MTR_3g071590 34.459 148 87 5 10 157 9 146 1.21e-21 88.2
MS.gene070516.t1 MTR_3g094690 37.273 110 60 3 1 110 42 142 2.09e-21 89.4
MS.gene070516.t1 MTR_3g071420 38.614 101 57 2 5 105 10 105 2.57e-21 87.8
MS.gene070516.t1 MTR_8g079620 40.367 109 61 2 3 111 5 109 2.91e-21 89.0
MS.gene070516.t1 MTR_1g095850 42.553 94 53 1 10 103 73 165 5.39e-21 88.2
MS.gene070516.t1 MTR_8g079660 40.367 109 61 2 3 111 5 109 1.52e-20 87.8
MS.gene070516.t1 MTR_5g080470 33.654 104 67 2 9 111 7 109 4.81e-17 76.6
MS.gene070516.t1 MTR_8g079580 37.615 109 63 3 3 110 5 109 1.99e-16 75.9
MS.gene070516.t1 MTR_1g070220 36.000 100 62 2 10 108 16 114 6.46e-16 73.9
MS.gene070516.t1 MTR_8g036105 35.922 103 64 2 10 111 19 120 7.55e-16 73.9
MS.gene070516.t1 MTR_7g096530 33.654 104 67 2 10 112 15 117 7.88e-16 73.9
MS.gene070516.t1 MTR_6g027710 33.898 118 68 4 3 114 37 150 1.44e-15 73.6
MS.gene070516.t1 MTR_8g009730 30.469 128 87 2 9 136 10 135 9.08e-15 70.9
MS.gene070516.t1 MTR_6g027700 30.645 124 78 3 10 126 8 130 1.68e-14 70.1
MS.gene070516.t1 MTR_7g074990 33.000 100 65 2 10 108 21 119 2.95e-14 70.1
MS.gene070516.t1 MTR_1g070205 34.783 115 65 5 10 123 13 118 4.81e-14 69.3
MS.gene070516.t1 MTR_8g036085 32.039 103 68 2 10 111 24 125 5.13e-14 69.3
MS.gene070516.t1 MTR_4g088035 25.157 159 108 2 9 157 6 163 1.13e-12 65.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070516.t1 AT3G47870 56.863 102 43 1 9 110 36 136 2.48e-36 130
MS.gene070516.t1 AT3G13850 48.246 114 58 1 8 121 35 147 7.96e-30 112
MS.gene070516.t1 AT1G65620 30.348 201 130 2 7 207 7 197 1.54e-27 104
MS.gene070516.t1 AT1G65620 30.348 201 130 2 7 207 7 197 1.54e-27 104
MS.gene070516.t1 AT1G65620 30.348 201 130 2 7 207 7 197 1.54e-27 104
MS.gene070516.t1 AT1G65620 30.348 201 130 2 7 207 7 197 1.54e-27 104
MS.gene070516.t1 AT1G65620 30.348 201 130 2 7 207 7 197 1.54e-27 104
MS.gene070516.t1 AT5G66870 44.118 102 56 1 10 111 8 108 1.20e-26 105
MS.gene070516.t1 AT3G27650 42.342 111 62 2 7 116 37 146 2.20e-26 100
MS.gene070516.t1 AT2G40470 34.247 146 84 5 1 145 15 149 1.90e-25 99.0
MS.gene070516.t1 AT2G40470 34.247 146 84 5 1 145 40 174 3.95e-25 99.0
MS.gene070516.t1 AT1G72980 42.975 121 67 2 9 128 13 132 5.77e-25 98.2
MS.gene070516.t1 AT5G63090 40.566 106 62 1 7 112 9 113 6.00e-25 97.4
MS.gene070516.t1 AT5G63090 40.566 106 62 1 7 112 9 113 6.00e-25 97.4
MS.gene070516.t1 AT5G63090 40.566 106 62 1 7 112 9 113 6.00e-25 97.4
MS.gene070516.t1 AT5G63090 40.566 106 62 1 7 112 9 113 6.00e-25 97.4
MS.gene070516.t1 AT2G30340 37.037 108 64 2 1 108 48 151 4.47e-24 97.1
MS.gene070516.t1 AT2G30340 37.037 108 64 2 1 108 47 150 4.82e-24 97.1
MS.gene070516.t1 AT1G31320 47.500 80 41 1 10 89 14 92 5.40e-24 94.7
MS.gene070516.t1 AT1G07900 48.750 80 40 1 10 89 34 112 1.17e-22 91.7
MS.gene070516.t1 AT2G23660 41.176 102 59 1 10 111 6 106 1.71e-22 94.0
MS.gene070516.t1 AT2G23660 41.176 102 59 1 10 111 6 106 1.71e-22 94.0
MS.gene070516.t1 AT2G23660 41.176 102 59 1 10 111 6 106 1.71e-22 94.0
MS.gene070516.t1 AT2G28500 48.750 80 40 1 10 89 56 134 3.56e-22 91.3
MS.gene070516.t1 AT3G26660 34.615 104 67 1 8 111 4 106 6.48e-22 87.8
MS.gene070516.t1 AT3G26620 32.174 115 77 1 8 122 4 117 7.45e-22 87.4
MS.gene070516.t1 AT2G30130 36.522 115 71 2 10 124 9 121 2.10e-21 88.2
MS.gene070516.t1 AT1G16530 34.921 126 76 4 10 133 15 136 2.16e-21 87.4
MS.gene070516.t1 AT1G06280 40.385 104 54 3 7 110 22 117 1.09e-20 86.7
MS.gene070516.t1 AT3G50510 39.091 110 65 2 5 113 8 116 1.25e-20 86.3
MS.gene070516.t1 AT3G50510 39.091 110 65 2 5 113 8 116 1.25e-20 86.3
MS.gene070516.t1 AT3G11090 35.644 101 60 2 5 105 7 102 3.04e-20 84.7
MS.gene070516.t1 AT5G15060 45.205 73 39 1 10 81 18 90 3.20e-17 75.9
MS.gene070516.t1 AT2G19820 40.000 80 48 0 10 89 13 92 5.70e-17 74.7
MS.gene070516.t1 AT4G00210 31.343 134 89 3 10 142 12 143 5.62e-16 74.3
MS.gene070516.t1 AT3G03760 35.294 102 64 2 10 110 52 152 1.08e-15 74.7
MS.gene070516.t1 AT5G35900 31.068 103 70 1 9 111 5 106 2.67e-15 72.4
MS.gene070516.t1 AT4G00220 32.673 101 66 2 12 111 20 119 6.54e-15 71.6
MS.gene070516.t1 AT2G42430 37.805 82 49 2 10 90 16 96 2.70e-14 70.5
MS.gene070516.t1 AT5G06080 30.597 134 81 4 9 141 7 129 8.95e-14 67.8
MS.gene070516.t1 AT2G31310 31.298 131 72 4 10 127 8 133 1.64e-13 67.0
MS.gene070516.t1 AT3G58190 32.039 103 68 2 10 111 12 113 4.77e-13 66.2
MS.gene070516.t1 AT2G45420 31.683 101 67 2 12 111 40 139 7.52e-13 66.6
MS.gene070516.t1 AT2G42440 30.303 99 67 2 10 107 8 105 9.47e-13 65.9
MS.gene070516.t1 AT2G45410 32.673 101 66 2 12 111 19 118 5.50e-12 63.2

Find 40 sgRNAs with CRISPR-Local

Find 47 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTAAATATTGGGATATT+TGG 0.141273 5.4:-67549233 None:intergenic
TCACCAACATTGTTAAATAT+TGG 0.190888 5.4:-67549242 None:intergenic
CTTGGTGTTATTATGAATTA+TGG 0.198268 5.4:+67549089 MS.gene070516:CDS
AGCTAGAACAACATTTGATT+TGG 0.264146 5.4:+67549481 MS.gene070516:CDS
TCATTGCTTGATAATCCTTT+TGG 0.267871 5.4:-67549413 None:intergenic
CATGCTGCACAAGCCTTGTT+TGG 0.269582 5.4:-67548849 None:intergenic
TCCTTTAGCTCCTTATTTCC+CGG 0.285923 5.4:+67548905 MS.gene070516:CDS
GGAATTTGAGAACTAGTATA+AGG 0.287802 5.4:-67549212 None:intergenic
AATATCCGACTCAATAATTA+TGG 0.288561 5.4:-67549039 None:intergenic
GCTAGAACAACATTTGATTT+GGG 0.292314 5.4:+67549482 MS.gene070516:CDS
CACCAACATTGTTAAATATT+GGG 0.294726 5.4:-67549241 None:intergenic
GGTTTATCGGCCGGGAAATA+AGG 0.295833 5.4:-67548915 None:intergenic
AGCAACGCTCATCGTTTGTT+TGG 0.383470 5.4:+67548948 MS.gene070516:CDS
GGATGACAATTCTAATTTGA+TGG 0.388591 5.4:+67549388 MS.gene070516:CDS
CCTCATGATGTTAACTATAG+TGG 0.400420 5.4:+67549350 MS.gene070516:CDS
AGATATGTTAAAGCAGTCTA+TGG 0.416875 5.4:+67549112 MS.gene070516:CDS
CTTTAGTATCCTTTGTATGT+TGG 0.435097 5.4:-67548976 None:intergenic
CGTGCAAAATCTCCCGTTCA+TGG 0.444453 5.4:+67549062 MS.gene070516:CDS
ATCCCAATATTTAACAATGT+TGG 0.449694 5.4:+67549239 MS.gene070516:CDS
GACGACCTTGACGACCTTGA+TGG 0.460725 5.4:+67549443 MS.gene070516:CDS
TTTGGTTTAATGATATTCAT+TGG 0.470812 5.4:-67548831 None:intergenic
TCTAATTTGATGGTACCAAA+AGG 0.475215 5.4:+67549398 MS.gene070516:CDS
ACACCAAGACATCCATGAAC+GGG 0.497015 5.4:-67549074 None:intergenic
TCTCCCGTTCATGGATGTCT+TGG 0.510462 5.4:+67549071 MS.gene070516:CDS
AAAGCAGTCTATGGAAGAGT+TGG 0.512156 5.4:+67549121 MS.gene070516:CDS
AAGGATACTAAAGGAAATCA+AGG 0.515917 5.4:+67548986 MS.gene070516:CDS
GCCGGGAAATAAGGAGCTAA+AGG 0.524058 5.4:-67548906 None:intergenic
CAACATACAAAGGATACTAA+AGG 0.551295 5.4:+67548977 MS.gene070516:CDS
CTAGAACAACATTTGATTTG+GGG 0.562151 5.4:+67549483 MS.gene070516:CDS
GAGATCCATAATTATTGAGT+CGG 0.580015 5.4:+67549034 MS.gene070516:CDS
CCACTATAGTTAACATCATG+AGG 0.583308 5.4:-67549350 None:intergenic
GTTCATCAACACCACCATCA+AGG 0.592727 5.4:-67549457 None:intergenic
TTGGTGTTTCCAACATACAA+AGG 0.602738 5.4:+67548967 MS.gene070516:CDS
AACACCAAGACATCCATGAA+CGG 0.603796 5.4:-67549075 None:intergenic
GAATATCATTAAACCAAACA+AGG 0.609165 5.4:+67548836 MS.gene070516:CDS
CACCACCATCAAGGTCGTCA+AGG 0.611148 5.4:-67549448 None:intergenic
CATGATGTTAACTATAGTGG+TGG 0.632189 5.4:+67549353 MS.gene070516:CDS
TAGTGGTGGCGACTATGTCA+TGG 0.648101 5.4:+67549367 MS.gene070516:CDS
CACTATAGTTAACATCATGA+GGG 0.659397 5.4:-67549349 None:intergenic
GACCTTGACGACCTTGATGG+TGG 0.717140 5.4:+67549446 MS.gene070516:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GGATAATAATAATAATAATT+AGG - chr5.4:67549272-67549291 None:intergenic 10.0%
!!! ATATTATTATTTTCACTATT+GGG - chr5.4:67549293-67549312 None:intergenic 10.0%
!!! CATATTATTATTTTCACTAT+TGG - chr5.4:67549294-67549313 None:intergenic 15.0%
!! TTGTTAAATATTGGGATATT+TGG - chr5.4:67549236-67549255 None:intergenic 20.0%
! AATATCCGACTCAATAATTA+TGG - chr5.4:67549042-67549061 None:intergenic 25.0%
! ATCCCAATATTTAACAATGT+TGG + chr5.4:67549239-67549258 MS.gene070516:CDS 25.0%
! CACCAACATTGTTAAATATT+GGG - chr5.4:67549244-67549263 None:intergenic 25.0%
! GAATATCATTAAACCAAACA+AGG + chr5.4:67548836-67548855 MS.gene070516:CDS 25.0%
! TCACCAACATTGTTAAATAT+TGG - chr5.4:67549245-67549264 None:intergenic 25.0%
!!! CTTGGTGTTATTATGAATTA+TGG + chr5.4:67549089-67549108 MS.gene070516:CDS 25.0%
!!! GCTAAACATTTTTGGTTTAT+CGG - chr5.4:67548931-67548950 None:intergenic 25.0%
AAGGATACTAAAGGAAATCA+AGG + chr5.4:67548986-67549005 MS.gene070516:CDS 30.0%
AGATATGTTAAAGCAGTCTA+TGG + chr5.4:67549112-67549131 MS.gene070516:CDS 30.0%
AGCTAGAACAACATTTGATT+TGG + chr5.4:67549481-67549500 MS.gene070516:CDS 30.0%
CAACATACAAAGGATACTAA+AGG + chr5.4:67548977-67548996 MS.gene070516:CDS 30.0%
CACTATAGTTAACATCATGA+GGG - chr5.4:67549352-67549371 None:intergenic 30.0%
CTTTAGTATCCTTTGTATGT+TGG - chr5.4:67548979-67548998 None:intergenic 30.0%
GAGATCCATAATTATTGAGT+CGG + chr5.4:67549034-67549053 MS.gene070516:CDS 30.0%
GGAATTTGAGAACTAGTATA+AGG - chr5.4:67549215-67549234 None:intergenic 30.0%
GGATGACAATTCTAATTTGA+TGG + chr5.4:67549388-67549407 MS.gene070516:CDS 30.0%
! GCTAGAACAACATTTGATTT+GGG + chr5.4:67549482-67549501 MS.gene070516:CDS 30.0%
! TCTAATTTGATGGTACCAAA+AGG + chr5.4:67549398-67549417 MS.gene070516:CDS 30.0%
!! ACATTTGATTTGGGGAAAAA+AGG + chr5.4:67549491-67549510 MS.gene070516:CDS 30.0%
!! CTAGAACAACATTTGATTTG+GGG + chr5.4:67549483-67549502 MS.gene070516:CDS 30.0%
!! TCATTGCTTGATAATCCTTT+TGG - chr5.4:67549416-67549435 None:intergenic 30.0%
CATGATGTTAACTATAGTGG+TGG + chr5.4:67549353-67549372 MS.gene070516:CDS 35.0%
CCACTATAGTTAACATCATG+AGG - chr5.4:67549353-67549372 None:intergenic 35.0%
CCTCATGATGTTAACTATAG+TGG + chr5.4:67549350-67549369 MS.gene070516:CDS 35.0%
! TTGGTGTTTCCAACATACAA+AGG + chr5.4:67548967-67548986 MS.gene070516:CDS 35.0%
!! TGAGCGTTGCTAAACATTTT+TGG - chr5.4:67548939-67548958 None:intergenic 35.0%
!!! AACATTTTTGGTTTATCGGC+CGG - chr5.4:67548927-67548946 None:intergenic 35.0%
AAAGCAGTCTATGGAAGAGT+TGG + chr5.4:67549121-67549140 MS.gene070516:CDS 40.0%
AACACCAAGACATCCATGAA+CGG - chr5.4:67549078-67549097 None:intergenic 40.0%
! TCCTTTAGCTCCTTATTTCC+CGG + chr5.4:67548905-67548924 MS.gene070516:CDS 40.0%
!!! ACATTTTTGGTTTATCGGCC+GGG - chr5.4:67548926-67548945 None:intergenic 40.0%
ACACCAAGACATCCATGAAC+GGG - chr5.4:67549077-67549096 None:intergenic 45.0%
AGCAACGCTCATCGTTTGTT+TGG + chr5.4:67548948-67548967 MS.gene070516:CDS 45.0%
GTTCATCAACACCACCATCA+AGG - chr5.4:67549460-67549479 None:intergenic 45.0%
CGTGCAAAATCTCCCGTTCA+TGG + chr5.4:67549062-67549081 MS.gene070516:CDS 50.0%
GCCGGGAAATAAGGAGCTAA+AGG - chr5.4:67548909-67548928 None:intergenic 50.0%
GGTTTATCGGCCGGGAAATA+AGG - chr5.4:67548918-67548937 None:intergenic 50.0%
TCTCCCGTTCATGGATGTCT+TGG + chr5.4:67549071-67549090 MS.gene070516:CDS 50.0%
! TAGTGGTGGCGACTATGTCA+TGG + chr5.4:67549367-67549386 MS.gene070516:CDS 50.0%
!! CATGCTGCACAAGCCTTGTT+TGG - chr5.4:67548852-67548871 None:intergenic 50.0%
CACCACCATCAAGGTCGTCA+AGG - chr5.4:67549451-67549470 None:intergenic 55.0%
GACCTTGACGACCTTGATGG+TGG + chr5.4:67549446-67549465 MS.gene070516:CDS 55.0%
GACGACCTTGACGACCTTGA+TGG + chr5.4:67549443-67549462 MS.gene070516:CDS 55.0%


Chromosome Type Strat End Strand Name
chr5.4 gene 67548834 67549535 67548834 ID=MS.gene070516
chr5.4 mRNA 67548834 67549535 67548834 ID=MS.gene070516.t1;Parent=MS.gene070516
chr5.4 exon 67548834 67549535 67548834 ID=MS.gene070516.t1.exon1;Parent=MS.gene070516.t1
chr5.4 CDS 67548834 67549535 67548834 ID=cds.MS.gene070516.t1;Parent=MS.gene070516.t1
Gene Sequence

>MS.gene070516

ATGAATATCATTAAACCAAACAAGGCTTGTGCAGCATGCAAGTATCAAAGAAGAAAGTGTTCAAGTGAGTGTCCTTTAGCTCCTTATTTCCCGGCCGATAAACCAAAAATGTTTAGCAACGCTCATCGTTTGTTTGGTGTTTCCAACATACAAAGGATACTAAAGGAAATCAAGGACGACGATCAAAAAGATGATGCGATGAGATCCATAATTATTGAGTCGGATATTCGTGCAAAATCTCCCGTTCATGGATGTCTTGGTGTTATTATGAATTATGGAGATATGTTAAAGCAGTCTATGGAAGAGTTGGATCATGTAAAAACGTTACTAGCTTATTGTAACGATTTACAACAAAAACAACATCTTCATTCTTCTTTACCTTATACTAGTTCTCAAATTCCAAATATCCCAATATTTAACAATGTTGGTGATGTTCCTAATTATTATTATTATTATCCCAATAGTGAAAATAATAATATGATGAGAGCATCTTCTTCTTATATTAGTGACATCATCCCTCATGATGTTAACTATAGTGGTGGCGACTATGTCATGGATGACAATTCTAATTTGATGGTACCAAAAGGATTATCAAGCAATGAAGAATCAGACGACCTTGACGACCTTGATGGTGGTGTTGATGAACAAGCTAGAACAACATTTGATTTGGGGAAAAAAGGAAGATTTTCAGATAGTGATTAA

Protein sequence

>MS.gene070516.t1

MNIIKPNKACAACKYQRRKCSSECPLAPYFPADKPKMFSNAHRLFGVSNIQRILKEIKDDDQKDDAMRSIIIESDIRAKSPVHGCLGVIMNYGDMLKQSMEELDHVKTLLAYCNDLQQKQHLHSSLPYTSSQIPNIPIFNNVGDVPNYYYYYPNSENNNMMRASSSYISDIIPHDVNYSGGDYVMDDNSNLMVPKGLSSNEESDDLDDLDGGVDEQARTTFDLGKKGRFSDSD