AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070578

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070578.t1 MTR_5g040740 73.536 461 70 5 1 460 1 410 0.0 644
MS.gene070578.t1 MTR_5g040880 58.588 524 140 9 1 456 1 515 0.0 546
MS.gene070578.t1 MTR_5g082140 57.602 467 172 5 1 450 1 458 1.13e-175 500
MS.gene070578.t1 MTR_2g024430 59.102 423 153 9 1 416 9 418 3.45e-155 450
MS.gene070578.t1 MTR_5g061220 47.092 533 192 10 1 456 8 527 8.73e-143 419
MS.gene070578.t1 MTR_1g058210 46.537 462 213 11 1 448 1 442 2.63e-120 359
MS.gene070578.t1 MTR_2g006270 45.720 479 199 12 4 454 5 450 1.49e-119 357
MS.gene070578.t1 MTR_2g006380 42.678 478 204 13 6 454 14 450 6.51e-108 327
MS.gene070578.t1 MTR_3g073420 40.173 463 224 10 2 421 9 461 2.91e-96 301
MS.gene070578.t1 MTR_5g060780 46.499 357 162 8 1 344 1 341 8.58e-92 284
MS.gene070578.t1 MTR_5g460920 46.499 357 162 8 1 344 1 341 8.33e-91 281
MS.gene070578.t1 MTR_5g061890 46.218 357 163 8 1 344 1 341 1.08e-87 273
MS.gene070578.t1 MTR_5g060630 40.412 485 205 12 1 411 1 475 5.39e-85 270
MS.gene070578.t1 MTR_5g460740 40.412 485 205 12 1 411 1 475 5.39e-85 270
MS.gene070578.t1 MTR_2g094570 34.874 476 231 11 9 424 8 464 3.71e-78 256
MS.gene070578.t1 MTR_2g094570 34.874 476 231 11 9 424 8 464 5.47e-78 256
MS.gene070578.t1 MTR_2g094570 34.874 476 231 11 9 424 64 520 1.91e-77 256
MS.gene070578.t1 MTR_1g064430 35.449 457 220 8 9 405 24 465 1.09e-75 250
MS.gene070578.t1 MTR_1g064430 35.449 457 220 8 9 405 37 478 1.54e-75 250
MS.gene070578.t1 MTR_4g058930 34.874 476 221 12 1 410 1 453 7.39e-74 245
MS.gene070578.t1 MTR_4g058930 34.874 476 221 12 1 410 1 453 1.59e-73 244
MS.gene070578.t1 MTR_1g094960 33.740 492 229 14 9 434 16 476 8.69e-73 241
MS.gene070578.t1 MTR_7g101280 32.627 472 228 13 9 411 28 478 3.24e-68 229
MS.gene070578.t1 MTR_0041s0110 32.627 472 228 13 9 411 28 478 3.24e-68 229
MS.gene070578.t1 MTR_5g062970 54.545 198 84 2 9 203 35 229 1.70e-65 211
MS.gene070578.t1 MTR_7g101275 40.070 287 139 7 23 292 3 273 4.95e-61 204
MS.gene070578.t1 MTR_0041s0120 37.755 294 148 6 23 292 3 285 8.74e-56 191
MS.gene070578.t1 MTR_2g093740 35.040 371 211 12 12 358 56 420 2.50e-50 182
MS.gene070578.t1 MTR_2g093740 35.040 371 211 12 12 358 56 420 4.19e-50 182
MS.gene070578.t1 MTR_2g093740 35.040 371 211 12 12 358 56 420 5.98e-50 182
MS.gene070578.t1 MTR_2g014770 32.591 359 185 11 12 337 53 387 2.58e-49 178
MS.gene070578.t1 MTR_2g014770 32.591 359 185 11 12 337 53 387 4.90e-49 178
MS.gene070578.t1 MTR_4g088210 33.242 364 180 12 12 337 38 376 9.42e-49 177
MS.gene070578.t1 MTR_4g088210 33.242 364 180 12 12 337 38 376 1.04e-48 177
MS.gene070578.t1 MTR_2g093740 33.854 384 211 13 12 358 56 433 8.64e-47 172
MS.gene070578.t1 MTR_2g093740 33.854 384 211 13 12 358 56 433 1.13e-46 172
MS.gene070578.t1 MTR_2g093740 33.854 384 211 13 12 358 56 433 1.45e-46 172
MS.gene070578.t1 MTR_8g100050 32.055 365 216 9 12 356 59 411 4.43e-46 171
MS.gene070578.t1 MTR_8g100050 32.055 365 216 9 12 356 59 411 4.51e-46 170
MS.gene070578.t1 MTR_4g021580 28.947 418 250 10 10 406 17 408 1.16e-45 168
MS.gene070578.t1 MTR_4g021580 28.947 418 250 10 10 406 17 408 1.73e-45 168
MS.gene070578.t1 MTR_4g060460 34.324 370 215 10 12 358 54 418 2.82e-45 169
MS.gene070578.t1 MTR_4g060460 34.324 370 215 10 12 358 54 418 2.97e-45 168
MS.gene070578.t1 MTR_5g060770 34.986 363 169 11 61 411 7 314 6.22e-45 160
MS.gene070578.t1 MTR_8g101360 30.874 366 194 11 9 340 28 368 8.86e-45 168
MS.gene070578.t1 MTR_2g018690 29.793 386 206 12 9 359 20 375 1.07e-43 164
MS.gene070578.t1 MTR_2g005240 32.723 382 212 10 12 364 23 388 3.72e-43 161
MS.gene070578.t1 MTR_7g062540 30.184 434 248 15 12 414 14 423 6.04e-43 161
MS.gene070578.t1 MTR_8g079492 30.585 376 215 12 9 359 20 374 7.50e-42 159
MS.gene070578.t1 MTR_8g079492 30.585 376 215 12 9 359 20 374 7.71e-42 159
MS.gene070578.t1 MTR_3g064050 30.343 379 213 12 9 359 23 378 2.46e-41 157
MS.gene070578.t1 MTR_2g043250 30.595 353 208 10 9 339 20 357 1.42e-40 155
MS.gene070578.t1 MTR_8g027440 32.378 349 194 11 12 337 29 358 1.52e-40 154
MS.gene070578.t1 MTR_4g124900 30.303 363 221 10 9 351 20 370 2.19e-40 155
MS.gene070578.t1 MTR_4g124900 30.303 363 221 10 9 351 20 370 2.26e-40 155
MS.gene070578.t1 MTR_5g076270 29.534 386 207 13 9 359 21 376 3.31e-40 154
MS.gene070578.t1 MTR_1g024025 29.661 354 207 9 9 337 40 376 3.84e-40 154
MS.gene070578.t1 MTR_2g093740 36.614 254 149 7 114 358 40 290 7.69e-36 140
MS.gene070578.t1 MTR_2g093740 36.614 254 149 7 114 358 40 290 1.11e-35 140
MS.gene070578.t1 MTR_2g093740 34.831 267 149 8 114 358 40 303 1.65e-32 130
MS.gene070578.t1 MTR_2g093740 34.831 267 149 8 114 358 40 303 2.18e-32 130
MS.gene070578.t1 MTR_8g079492 30.921 304 180 10 72 359 3 292 2.52e-32 130
MS.gene070578.t1 MTR_5g460910 30.667 375 168 15 51 411 13 309 1.56e-26 110
MS.gene070578.t1 MTR_8g446900 26.879 346 200 11 61 375 12 335 6.35e-24 103
MS.gene070578.t1 MTR_5g062960 36.559 186 89 4 235 415 2 163 7.79e-23 96.3
MS.gene070578.t1 MTR_5g046120 40.351 114 59 4 53 161 15 124 3.90e-15 72.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070578.t1 AT1G77850 36.923 455 215 10 9 410 16 451 1.63e-91 289
MS.gene070578.t1 AT1G77850 36.923 455 215 10 9 410 16 451 3.10e-91 289
MS.gene070578.t1 AT4G30080 35.000 460 213 9 9 406 16 451 2.65e-74 246
MS.gene070578.t1 AT2G28350 37.530 421 174 12 4 356 2 401 1.39e-72 242
MS.gene070578.t1 AT2G28350 37.530 421 174 12 4 356 2 401 1.60e-72 241
MS.gene070578.t1 AT2G33860 34.097 349 193 10 12 337 54 388 2.07e-51 184
MS.gene070578.t1 AT5G60450 36.441 354 190 12 9 337 38 381 9.72e-47 172
MS.gene070578.t1 AT4G23980 31.534 352 202 9 12 341 12 346 1.04e-46 171
MS.gene070578.t1 AT4G23980 31.534 352 202 9 12 341 12 346 1.07e-46 171
MS.gene070578.t1 AT5G60450 36.441 354 190 12 9 337 62 405 1.13e-46 172
MS.gene070578.t1 AT1G35520 33.155 374 201 13 12 360 27 376 3.85e-45 166
MS.gene070578.t1 AT5G62000 30.997 371 214 9 12 357 61 414 5.28e-44 165
MS.gene070578.t1 AT5G62000 30.997 371 214 9 12 357 61 414 5.28e-44 165
MS.gene070578.t1 AT5G62000 30.997 371 214 9 12 357 61 414 5.28e-44 165
MS.gene070578.t1 AT5G62000 30.997 371 214 9 12 357 61 414 5.28e-44 165
MS.gene070578.t1 AT5G62000 30.997 371 214 9 12 357 61 414 5.54e-44 165
MS.gene070578.t1 AT2G46530 31.180 356 195 10 12 340 21 353 3.71e-43 160
MS.gene070578.t1 AT5G37020 30.890 382 233 12 9 370 19 389 3.79e-43 162
MS.gene070578.t1 AT5G37020 30.890 382 233 12 9 370 19 389 5.80e-43 162
MS.gene070578.t1 AT3G61830 31.250 352 194 8 12 337 25 354 6.23e-43 160
MS.gene070578.t1 AT2G46530 31.180 356 195 10 12 340 42 374 7.67e-43 160
MS.gene070578.t1 AT1G30330 29.319 382 213 10 9 359 22 377 1.16e-42 161
MS.gene070578.t1 AT1G30330 29.319 382 213 10 9 359 22 377 1.16e-42 161
MS.gene070578.t1 AT1G30330 29.319 382 213 10 9 359 20 375 1.36e-42 161
MS.gene070578.t1 AT1G59750 30.623 369 212 9 12 354 22 372 2.89e-42 159
MS.gene070578.t1 AT1G34410 31.283 374 208 13 12 360 27 376 3.31e-42 158
MS.gene070578.t1 AT1G59750 30.623 369 212 9 12 354 22 372 3.32e-42 159
MS.gene070578.t1 AT1G59750 30.623 369 212 9 12 354 22 372 3.32e-42 159
MS.gene070578.t1 AT1G59750 30.623 369 212 9 12 354 22 372 3.44e-42 159
MS.gene070578.t1 AT1G35240 31.016 374 209 12 12 360 27 376 4.57e-42 157
MS.gene070578.t1 AT1G19220 29.793 386 203 12 9 356 20 375 4.90e-42 160
MS.gene070578.t1 AT1G34390 30.481 374 209 13 12 360 27 374 2.76e-41 155
MS.gene070578.t1 AT1G34310 30.749 374 210 11 12 360 27 376 6.56e-41 154
MS.gene070578.t1 AT1G19850 30.659 349 211 9 9 337 40 377 1.21e-39 152
MS.gene070578.t1 AT1G19850 30.659 349 211 9 9 337 51 388 1.33e-39 152
MS.gene070578.t1 AT1G35540 31.143 350 193 11 12 337 27 352 1.98e-39 150
MS.gene070578.t1 AT5G20730 30.163 368 220 10 9 353 21 374 1.08e-38 150
MS.gene070578.t1 AT5G20730 30.163 368 220 10 9 353 21 374 1.20e-38 150
MS.gene070578.t1 AT5G20730 30.435 368 218 11 9 353 21 373 3.41e-38 149
MS.gene070578.t1 AT2G33860 35.443 237 131 7 114 337 4 231 5.69e-37 141
MS.gene070578.t1 AT1G34170 29.830 352 197 11 12 337 25 352 6.26e-34 134
MS.gene070578.t1 AT3G61830 31.457 302 162 7 59 337 24 303 6.89e-33 131
MS.gene070578.t1 AT1G34170 32.950 261 144 7 12 255 25 271 1.24e-31 127
MS.gene070578.t1 AT1G34170 32.950 261 144 7 12 255 25 271 2.71e-31 126
MS.gene070578.t1 AT5G37020 32.210 267 166 8 114 370 33 294 8.26e-31 126
MS.gene070578.t1 AT1G43950 35.981 214 112 6 12 218 27 222 1.93e-30 117
MS.gene070578.t1 AT2G46530 31.452 248 129 7 114 340 39 266 3.74e-28 117

Find 118 sgRNAs with CRISPR-Local

Find 165 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTCAATATCATAGGTTAAT+TGG 0.114277 5.3:+28544004 MS.gene070578:intron
AGGATTCCATTTAATCTTAA+TGG 0.197798 5.3:-28543350 None:intergenic
CCTGGTTCAATTATGTTATT+TGG 0.201738 5.3:+28544336 MS.gene070578:CDS
GGGAGAGTGTGCTAGGGTTT+GGG 0.238185 5.3:-28542676 None:intergenic
CATGTTTACTACTTCCCATT+AGG 0.255048 5.3:+28542629 MS.gene070578:CDS
GAAGAAAGAAACTCTATCTT+TGG 0.259664 5.3:-28544189 None:intergenic
AGGGAGAGTGTGCTAGGGTT+TGG 0.295398 5.3:-28542677 None:intergenic
ATGTGTTCTAAGTGCCCTAA+TGG 0.313804 5.3:-28542644 None:intergenic
AGGAAGCCATTAAGATTAAA+TGG 0.321373 5.3:+28543344 MS.gene070578:CDS
TAAACATGAGAATGTAGTTT+TGG 0.329887 5.3:-28542614 None:intergenic
GGTCGTTAGTGACGGGAGTT+AGG 0.352092 5.3:-28542793 None:intergenic
GGGGTTGAGGTTGTTTCCTC+TGG 0.358289 5.3:+28542857 MS.gene070578:CDS
ATGTTTACTACTTCCCATTA+GGG 0.360944 5.3:+28542630 MS.gene070578:CDS
CCGCATCAACTTCTTCCAAC+TGG 0.361848 5.3:+28544295 MS.gene070578:CDS
GTCAATATCATAGGTTAATT+GGG 0.372441 5.3:+28544005 MS.gene070578:intron
GGAATCCGCCGGCAGAAGTT+TGG 0.393817 5.3:+28543187 MS.gene070578:CDS
AAGAAAGAAACTCTATCTTT+GGG 0.407439 5.3:-28544188 None:intergenic
TTCTAGAGCGAGTCAGTCCT+TGG 0.420887 5.3:+28544049 MS.gene070578:CDS
ATTCAACGATGTTTCATTGA+AGG 0.425033 5.3:-28544223 None:intergenic
AAAATCTGGCAGTGCTGCGC+CGG 0.426459 5.3:+28542575 MS.gene070578:CDS
AAAGAGATGCTCAAAATTTG+AGG 0.427251 5.3:+28543413 MS.gene070578:CDS
CACTCCAGTTAGTGGTGATC+AGG 0.431295 5.3:-28543066 None:intergenic
GGTTACCGAGGCTGTAGAAT+TGG 0.432772 5.3:+28543237 MS.gene070578:CDS
AGCATGCGCCATGGACGATT+AGG 0.434996 5.3:-28543459 None:intergenic
ATTATCCTACTGCTGAAGTT+TGG 0.436257 5.3:+28543290 MS.gene070578:CDS
GACACCTGATCACCACTAAC+TGG 0.440049 5.3:+28543062 MS.gene070578:CDS
TGCTTTGCTCGATGACATGC+AGG 0.446126 5.3:+28544251 MS.gene070578:CDS
GAAAGTTTGATTCTCAATCC+AGG 0.447460 5.3:-28543370 None:intergenic
TTCGTGAAGACGAAGATGAC+GGG 0.459706 5.3:+28542837 MS.gene070578:CDS
TGTAGTTAATGCTAAGGTTG+TGG 0.462109 5.3:+28543321 MS.gene070578:CDS
ATTAAGATTAAATGGAATCC+TGG 0.463670 5.3:+28543352 MS.gene070578:CDS
AACAAACTCACTCCAGTTAG+TGG 0.468954 5.3:-28543074 None:intergenic
TTTACACGATTCAGATATCA+AGG 0.473052 5.3:+28544386 MS.gene070578:CDS
TCGATGACATGCAGGGAGCC+AGG 0.473164 5.3:+28544259 MS.gene070578:CDS
TGCCTTACGCAATGATATAG+AGG 0.477833 5.3:+28544152 MS.gene070578:CDS
AGGACATTCACGGCAAAGAA+TGG 0.478604 5.3:+28543005 MS.gene070578:CDS
TGGAGAAGGGAGAGTGTGCT+AGG 0.478757 5.3:-28542683 None:intergenic
GGAGAGTGTGCTAGGGTTTG+GGG 0.481560 5.3:-28542675 None:intergenic
ATCAACCGCGGAGACGGTGC+AGG 0.485860 5.3:-28542726 None:intergenic
GAGACGGTGCAGGGAATGAA+TGG 0.491412 5.3:-28542716 None:intergenic
TCTTCGTCTTCACGAACCTC+AGG 0.495857 5.3:-28542830 None:intergenic
TGTGTTCTAAGTGCCCTAAT+GGG 0.500812 5.3:-28542643 None:intergenic
AAATCTGGCAGTGCTGCGCC+GGG 0.508255 5.3:+28542576 MS.gene070578:CDS
TCGTGTAAATCACTTCCAAC+AGG 0.511200 5.3:-28544372 None:intergenic
AAGAGATGCTCAAAATTTGA+GGG 0.513384 5.3:+28543414 MS.gene070578:CDS
TCTAGAGCGAGTCAGTCCTT+GGG 0.516580 5.3:+28544050 MS.gene070578:CDS
ATTAATCTTGCGCATTCGCT+AGG 0.519508 5.3:-28542929 None:intergenic
AGCACACTCTCCCTTCTCCA+TGG 0.523233 5.3:+28542686 MS.gene070578:CDS
AGACGAAGATGACGGGGTTG+AGG 0.523787 5.3:+28542844 MS.gene070578:CDS
CTCCGCCAATTCTACAGCCT+CGG 0.528093 5.3:-28543242 None:intergenic
CAACTTGGAGGTCAAGTGGC+GGG 0.528362 5.3:-28542955 None:intergenic
CCTCAGGAGGTTCGTGGACA+CGG 0.532546 5.3:-28542814 None:intergenic
GATAGAGTTTCTTTCTTCAG+CGG 0.534938 5.3:+28544194 MS.gene070578:CDS
TGGCAGCACCAAACTTCTGC+CGG 0.534995 5.3:-28543195 None:intergenic
CACGAACCTCAGGAGGTTCG+TGG 0.537961 5.3:-28542820 None:intergenic
GATTCCAACGGAGATCGTTA+CGG 0.541776 5.3:-28543170 None:intergenic
GTCTCAAGAACTCTCAGTAA+AGG 0.546981 5.3:+28542985 MS.gene070578:CDS
GTTGACGAGAAGAAACTGAT+TGG 0.549713 5.3:+28543094 MS.gene070578:CDS
GAGTAGATCAACCGCGGAGA+CGG 0.550031 5.3:-28542732 None:intergenic
TCGTCCATGGCGCATGCTTG+AGG 0.553392 5.3:+28543464 MS.gene070578:CDS
AAGAAACTCTATCTTTGGGA+TGG 0.558090 5.3:-28544184 None:intergenic
TACCGAGGCTGTAGAATTGG+CGG 0.561717 5.3:+28543240 MS.gene070578:CDS
AGCTTCGCCACGTCTACCGT+GGG 0.564056 5.3:+28543029 MS.gene070578:CDS
GTGGACACGGTCGTTAGTGA+CGG 0.566445 5.3:-28542801 None:intergenic
AGTGTGCTAGGGTTTGGGGA+TGG 0.567118 5.3:-28542671 None:intergenic
CGGTGCAGGGAATGAATGGA+CGG 0.567424 5.3:-28542712 None:intergenic
AGTGGTGATCAGGTGTCGCA+AGG 0.570426 5.3:-28543056 None:intergenic
GAATGAATGGACGGGAACCA+TGG 0.573275 5.3:-28542703 None:intergenic
GGATGAACATAAAGCCTCGC+AGG 0.575663 5.3:+28544026 MS.gene070578:CDS
CGATCTCCGTTGGAATCCGC+CGG 0.580469 5.3:+28543176 MS.gene070578:CDS
ATGGACGGGAACCATGGAGA+AGG 0.582820 5.3:-28542697 None:intergenic
GTTAGGTTGCTGAGGAGACA+TGG 0.585884 5.3:-28542540 None:intergenic
GAAATAAGCTCAACCTCCCA+AGG 0.586576 5.3:-28544066 None:intergenic
CTCTCAGTAAAGGACATTCA+CGG 0.586911 5.3:+28542995 MS.gene070578:CDS
TGGACGGGAACCATGGAGAA+GGG 0.587085 5.3:-28542696 None:intergenic
GGTCAGATCATACGTCCTGT+TGG 0.588139 5.3:+28544357 MS.gene070578:CDS
TGGACACGGTCGTTAGTGAC+GGG 0.588676 5.3:-28542800 None:intergenic
TCCCTCTATATCATTGCGTA+AGG 0.589411 5.3:-28544154 None:intergenic
TCGTCTTCACGAACCTCAGG+AGG 0.589597 5.3:-28542827 None:intergenic
CCAAATAACATAATTGAACC+AGG 0.590972 5.3:-28544336 None:intergenic
TCACACCAAACTTCAGCAGT+AGG 0.591735 5.3:-28543295 None:intergenic
TATCTGCTCCTAATCGTCCA+TGG 0.595153 5.3:+28543451 MS.gene070578:CDS
ACTCCGTCTGATGCTAATAA+CGG 0.598328 5.3:+28542890 MS.gene070578:CDS
TACACGATTCAGATATCAAG+GGG 0.600673 5.3:+28544388 MS.gene070578:CDS
TGGATGCAGTGCATGTATGT+TGG 0.601398 5.3:-28544088 None:intergenic
GTTCGTGAAGACGAAGATGA+CGG 0.606081 5.3:+28542836 MS.gene070578:CDS
GTGGTGATCAGGTGTCGCAA+GGG 0.611088 5.3:-28543055 None:intergenic
AGAATTGGCGGAGAAGAACA+TGG 0.611636 5.3:+28543252 MS.gene070578:CDS
GCCTTACGCAATGATATAGA+GGG 0.612343 5.3:+28544153 MS.gene070578:CDS
ACTGCCGTAACGATCTCCGT+TGG 0.613324 5.3:+28543166 MS.gene070578:CDS
AGTTAATGCTAAGGTTGTGG+AGG 0.615178 5.3:+28543324 MS.gene070578:CDS
TCTTGCGCATTCGCTAGGTA+CGG 0.617532 5.3:-28542924 None:intergenic
AGGGAGTCCCACGGTAGACG+TGG 0.627277 5.3:-28543036 None:intergenic
CTTCTGCCGGCGGATTCCAA+CGG 0.627932 5.3:-28543182 None:intergenic
TATACCTCAAGCATGCGCCA+TGG 0.631418 5.3:-28543468 None:intergenic
GGTGCAGGGAATGAATGGAC+GGG 0.631616 5.3:-28542711 None:intergenic
AGAGCGAGTCAGTCCTTGGG+AGG 0.631664 5.3:+28544053 MS.gene070578:CDS
CAGCACCAAACTTCTGCCGG+CGG 0.635134 5.3:-28543192 None:intergenic
TTACACGATTCAGATATCAA+GGG 0.635502 5.3:+28544387 MS.gene070578:CDS
TCAACCGCGGAGACGGTGCA+GGG 0.637633 5.3:-28542725 None:intergenic
CCAGTTGGAAGAAGTTGATG+CGG 0.638085 5.3:-28544295 None:intergenic
TTTACTGAGAGTTCTTGAGA+CGG 0.640719 5.3:-28542983 None:intergenic
TCAATACGGTTAGGTTGCTG+AGG 0.643017 5.3:-28542548 None:intergenic
AAGCTTCGCCACGTCTACCG+TGG 0.643601 5.3:+28543028 MS.gene070578:CDS
TAGTTTGACGAAGACTTCGT+CGG 0.645961 5.3:-28542768 None:intergenic
CCGTGTCCACGAACCTCCTG+AGG 0.646741 5.3:+28542814 MS.gene070578:CDS
GGAGAAGGGAGAGTGTGCTA+GGG 0.651561 5.3:-28542682 None:intergenic
TCAACTTGGAGGTCAAGTGG+CGG 0.652917 5.3:-28542956 None:intergenic
GACGAGAAGAAACTGATTGG+TGG 0.658536 5.3:+28543097 MS.gene070578:CDS
GCTTTGCTCGATGACATGCA+GGG 0.669755 5.3:+28544252 MS.gene070578:CDS
CCACCGTTATTAGCATCAGA+CGG 0.670947 5.3:-28542893 None:intergenic
GTCAGCGAGTAGATCAACCG+CGG 0.673456 5.3:-28542738 None:intergenic
CATTCCCTGCACCGTCTCCG+CGG 0.679524 5.3:+28542721 MS.gene070578:CDS
GGTGTCGCAAGGGAGTCCCA+CGG 0.699974 5.3:-28543045 None:intergenic
TCGTGAAGACGAAGATGACG+GGG 0.700053 5.3:+28542838 MS.gene070578:CDS
GACTCGCTCTAGAACCTGCG+AGG 0.710261 5.3:-28544040 None:intergenic
CCGTCTGATGCTAATAACGG+TGG 0.711385 5.3:+28542893 MS.gene070578:CDS
ACACGATTCAGATATCAAGG+GGG 0.776881 5.3:+28544389 MS.gene070578:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAGTTTTTTTTTCTTTTTTT+GGG + chr5.3:28543810-28543829 MS.gene070578:intron 10.0%
!!! TAAGTTTTTTTTTCTTTTTT+TGG + chr5.3:28543809-28543828 MS.gene070578:intron 10.0%
!!! TTTTGTTTAATAAATGTTAT+CGG + chr5.3:28543561-28543580 MS.gene070578:intron 10.0%
!! AGAAACTTTATACTTGAAAT+AGG + chr5.3:28543781-28543800 MS.gene070578:intron 20.0%
!! ATTATAGTTAATTCATGATG+TGG - chr5.3:28543755-28543774 None:intergenic 20.0%
!! ATTGTGCAATTAAATTTAAG+TGG + chr5.3:28543701-28543720 MS.gene070578:intron 20.0%
!! CAAAATTCGTTATATTTACA+GGG - chr5.3:28543918-28543937 None:intergenic 20.0%
!! GTATGTACAATATACTTTAT+TGG + chr5.3:28543874-28543893 MS.gene070578:intron 20.0%
!! TCAAAATTCGTTATATTTAC+AGG - chr5.3:28543919-28543938 None:intergenic 20.0%
!!! AATGTTTAAGATTAACACAT+TGG - chr5.3:28543678-28543697 None:intergenic 20.0%
!!! ATATCCTATATTTTTCACAT+TGG - chr5.3:28543652-28543671 None:intergenic 20.0%
!!! ATGTAGTTTTGGAATTTTAA+AGG - chr5.3:28542606-28542625 None:intergenic 20.0%
!!! ATTTTTCATGTCAATATCAT+AGG + chr5.3:28543996-28544015 MS.gene070578:intron 20.0%
!!! GATGTTTTAAATGATTGATA+CGG + chr5.3:28543964-28543983 MS.gene070578:intron 20.0%
!!! TGATTTTTTCATGAAAACTA+AGG - chr5.3:28543128-28543147 None:intergenic 20.0%
! AAGAAAGAAACTCTATCTTT+GGG - chr5.3:28544191-28544210 None:intergenic 25.0%
! AGGATTCCATTTAATCTTAA+TGG - chr5.3:28543353-28543372 None:intergenic 25.0%
! ATTAAGATTAAATGGAATCC+TGG + chr5.3:28543352-28543371 MS.gene070578:CDS 25.0%
! CACTAAGATAACAGAAAAAT+CGG + chr5.3:28543216-28543235 MS.gene070578:CDS 25.0%
! GTCAATATCATAGGTTAATT+GGG + chr5.3:28544005-28544024 MS.gene070578:intron 25.0%
! TGTCAATATCATAGGTTAAT+TGG + chr5.3:28544004-28544023 MS.gene070578:intron 25.0%
! TGTTCCAATGTGAAAAATAT+AGG + chr5.3:28543645-28543664 MS.gene070578:intron 25.0%
!! TAAACATGAGAATGTAGTTT+TGG - chr5.3:28542617-28542636 None:intergenic 25.0%
!! TGATTTTGTAGTTAATGCTA+AGG + chr5.3:28543315-28543334 MS.gene070578:CDS 25.0%
!!! TTTTTCTTTTTTTGGGCAAA+TGG + chr5.3:28543817-28543836 MS.gene070578:intron 25.0%
!!! TTTTTTGATCAGTTCATGTT+AGG + chr5.3:28543615-28543634 MS.gene070578:intron 25.0%
AAAGAGATGCTCAAAATTTG+AGG + chr5.3:28543413-28543432 MS.gene070578:CDS 30.0%
AACACCAAAAAATTTAGTCC+TGG + chr5.3:28544318-28544337 MS.gene070578:CDS 30.0%
AAGAGATGCTCAAAATTTGA+GGG + chr5.3:28543414-28543433 MS.gene070578:CDS 30.0%
AATGTTATCGGTCAAAGTTA+TGG + chr5.3:28543573-28543592 MS.gene070578:intron 30.0%
AGGAAGCCATTAAGATTAAA+TGG + chr5.3:28543344-28543363 MS.gene070578:CDS 30.0%
ATGTTTACTACTTCCCATTA+GGG + chr5.3:28542630-28542649 MS.gene070578:CDS 30.0%
ATTCAACGATGTTTCATTGA+AGG - chr5.3:28544226-28544245 None:intergenic 30.0%
CCAAATAACATAATTGAACC+AGG - chr5.3:28544339-28544358 None:intergenic 30.0%
CCTGGTTCAATTATGTTATT+TGG + chr5.3:28544336-28544355 MS.gene070578:CDS 30.0%
GAAGAAAGAAACTCTATCTT+TGG - chr5.3:28544192-28544211 None:intergenic 30.0%
TCAAAGTTATGGTTCATGTT+AGG + chr5.3:28543584-28543603 MS.gene070578:intron 30.0%
TTACACGATTCAGATATCAA+GGG + chr5.3:28544387-28544406 MS.gene070578:CDS 30.0%
TTTACACGATTCAGATATCA+AGG + chr5.3:28544386-28544405 MS.gene070578:CDS 30.0%
! CGATTTTTCTGTTATCTTAG+TGG - chr5.3:28543218-28543237 None:intergenic 30.0%
! GTTTTCATGAAAAAATCAGC+TGG + chr5.3:28543130-28543149 MS.gene070578:CDS 30.0%
! TATTTTCATTGCATCCTCTA+CGG - chr5.3:28544422-28544441 None:intergenic 30.0%
!! ATTTTTGGATCAATACGGTT+AGG - chr5.3:28542560-28542579 None:intergenic 30.0%
!!! AAATTTTTTGGTGTTCCAGT+TGG - chr5.3:28544313-28544332 None:intergenic 30.0%
!!! TGAACCAGGACTAAATTTTT+TGG - chr5.3:28544325-28544344 None:intergenic 30.0%
ATGAAAAAATCAGCTGGAAC+TGG + chr5.3:28543136-28543155 MS.gene070578:CDS 35.0%
ATTATCCTACTGCTGAAGTT+TGG + chr5.3:28543290-28543309 MS.gene070578:CDS 35.0%
CATGTTTACTACTTCCCATT+AGG + chr5.3:28542629-28542648 MS.gene070578:CDS 35.0%
GAAAGTTTGATTCTCAATCC+AGG - chr5.3:28543373-28543392 None:intergenic 35.0%
GATAGAGTTTCTTTCTTCAG+CGG + chr5.3:28544194-28544213 MS.gene070578:CDS 35.0%
GGTACGATATGACTCAAAAA+AGG - chr5.3:28543734-28543753 None:intergenic 35.0%
TACACGATTCAGATATCAAG+GGG + chr5.3:28544388-28544407 MS.gene070578:CDS 35.0%
TGTAGTTAATGCTAAGGTTG+TGG + chr5.3:28543321-28543340 MS.gene070578:CDS 35.0%
TTTACTGAGAGTTCTTGAGA+CGG - chr5.3:28542986-28543005 None:intergenic 35.0%
! AAGAAACTCTATCTTTGGGA+TGG - chr5.3:28544187-28544206 None:intergenic 35.0%
! ACCGTATTGATCCAAAAATC+TGG + chr5.3:28542561-28542580 MS.gene070578:CDS 35.0%
!! GCCAGATTTTTGGATCAATA+CGG - chr5.3:28542565-28542584 None:intergenic 35.0%
!!! TCCTAAGCTTTTTTGTTCGA+GGG - chr5.3:28544119-28544138 None:intergenic 35.0%
AACAAACTCACTCCAGTTAG+TGG - chr5.3:28543077-28543096 None:intergenic 40.0%
AACAGAAAAATCGGTTACCG+AGG + chr5.3:28543225-28543244 MS.gene070578:CDS 40.0%
ACACGATTCAGATATCAAGG+GGG + chr5.3:28544389-28544408 MS.gene070578:CDS 40.0%
ACTCCGTCTGATGCTAATAA+CGG + chr5.3:28542890-28542909 MS.gene070578:CDS 40.0%
AGTTAATGCTAAGGTTGTGG+AGG + chr5.3:28543324-28543343 MS.gene070578:CDS 40.0%
ATTAATCTTGCGCATTCGCT+AGG - chr5.3:28542932-28542951 None:intergenic 40.0%
CTCTCAGTAAAGGACATTCA+CGG + chr5.3:28542995-28543014 MS.gene070578:CDS 40.0%
GCCTTACGCAATGATATAGA+GGG + chr5.3:28544153-28544172 MS.gene070578:CDS 40.0%
GTCTCAAGAACTCTCAGTAA+AGG + chr5.3:28542985-28543004 MS.gene070578:CDS 40.0%
GTTGACGAGAAGAAACTGAT+TGG + chr5.3:28543094-28543113 MS.gene070578:CDS 40.0%
TAGTTTGACGAAGACTTCGT+CGG - chr5.3:28542771-28542790 None:intergenic 40.0%
TCCCTCGAACAAAAAAGCTT+AGG + chr5.3:28544115-28544134 MS.gene070578:CDS 40.0%
TCCCTCTATATCATTGCGTA+AGG - chr5.3:28544157-28544176 None:intergenic 40.0%
TCGTGTAAATCACTTCCAAC+AGG - chr5.3:28544375-28544394 None:intergenic 40.0%
TGCCTTACGCAATGATATAG+AGG + chr5.3:28544152-28544171 MS.gene070578:CDS 40.0%
! ATGTGTTCTAAGTGCCCTAA+TGG - chr5.3:28542647-28542666 None:intergenic 40.0%
! TGTGTTCTAAGTGCCCTAAT+GGG - chr5.3:28542646-28542665 None:intergenic 40.0%
! TTTTCAACTTGGAGGTCAAG+TGG - chr5.3:28542962-28542981 None:intergenic 40.0%
!! TGGAATTTTAAAGGCAGACC+CGG - chr5.3:28542597-28542616 None:intergenic 40.0%
!!! GTCCTAAGCTTTTTTGTTCG+AGG - chr5.3:28544120-28544139 None:intergenic 40.0%
!!! TTTTTTGTTCGAGGGAACTG+TGG - chr5.3:28544111-28544130 None:intergenic 40.0%
AGAATTGGCGGAGAAGAACA+TGG + chr5.3:28543252-28543271 MS.gene070578:CDS 45.0%
AGGACATTCACGGCAAAGAA+TGG + chr5.3:28543005-28543024 MS.gene070578:CDS 45.0%
CCACCGTTATTAGCATCAGA+CGG - chr5.3:28542896-28542915 None:intergenic 45.0%
GAAATAAGCTCAACCTCCCA+AGG - chr5.3:28544069-28544088 None:intergenic 45.0%
GACGAGAAGAAACTGATTGG+TGG + chr5.3:28543097-28543116 MS.gene070578:CDS 45.0%
GATTCCAACGGAGATCGTTA+CGG - chr5.3:28543173-28543192 None:intergenic 45.0%
GTTCGTGAAGACGAAGATGA+CGG + chr5.3:28542836-28542855 MS.gene070578:CDS 45.0%
TATCTGCTCCTAATCGTCCA+TGG + chr5.3:28543451-28543470 MS.gene070578:CDS 45.0%
TCACACCAAACTTCAGCAGT+AGG - chr5.3:28543298-28543317 None:intergenic 45.0%
TGCGGAAAAAAGATTGTGCC+TGG - chr5.3:28544280-28544299 None:intergenic 45.0%
TGGATGCAGTGCATGTATGT+TGG - chr5.3:28544091-28544110 None:intergenic 45.0%
TTCGTGAAGACGAAGATGAC+GGG + chr5.3:28542837-28542856 MS.gene070578:CDS 45.0%
! CTTGAGACGGCTTTTCAACT+TGG - chr5.3:28542973-28542992 None:intergenic 45.0%
! TCAATACGGTTAGGTTGCTG+AGG - chr5.3:28542551-28542570 None:intergenic 45.0%
!! CCAGTTGGAAGAAGTTGATG+CGG - chr5.3:28544298-28544317 None:intergenic 45.0%
CACTCCAGTTAGTGGTGATC+AGG - chr5.3:28543069-28543088 None:intergenic 50.0%
CCGCATCAACTTCTTCCAAC+TGG + chr5.3:28544295-28544314 MS.gene070578:CDS 50.0%
CCGTCTGATGCTAATAACGG+TGG + chr5.3:28542893-28542912 MS.gene070578:CDS 50.0%
CGATTCAGATATCAAGGGGG+AGG + chr5.3:28544392-28544411 MS.gene070578:CDS 50.0%
GAATGAATGGACGGGAACCA+TGG - chr5.3:28542706-28542725 None:intergenic 50.0%
GACACCTGATCACCACTAAC+TGG + chr5.3:28543062-28543081 MS.gene070578:CDS 50.0%
GCTTTGCTCGATGACATGCA+GGG + chr5.3:28544252-28544271 MS.gene070578:CDS 50.0%
GGATGAACATAAAGCCTCGC+AGG + chr5.3:28544026-28544045 MS.gene070578:CDS 50.0%
GGTCAGATCATACGTCCTGT+TGG + chr5.3:28544357-28544376 MS.gene070578:CDS 50.0%
GGTTACCGAGGCTGTAGAAT+TGG + chr5.3:28543237-28543256 MS.gene070578:CDS 50.0%
TACCGAGGCTGTAGAATTGG+CGG + chr5.3:28543240-28543259 MS.gene070578:CDS 50.0%
TATACCTCAAGCATGCGCCA+TGG - chr5.3:28543471-28543490 None:intergenic 50.0%
TCAACTTGGAGGTCAAGTGG+CGG - chr5.3:28542959-28542978 None:intergenic 50.0%
TCAGATATCAAGGGGGAGGA+TGG + chr5.3:28544396-28544415 MS.gene070578:CDS 50.0%
TCGTGAAGACGAAGATGACG+GGG + chr5.3:28542838-28542857 MS.gene070578:CDS 50.0%
TCTTCGTCTTCACGAACCTC+AGG - chr5.3:28542833-28542852 None:intergenic 50.0%
TCTTGCGCATTCGCTAGGTA+CGG - chr5.3:28542927-28542946 None:intergenic 50.0%
TGCTTTGCTCGATGACATGC+AGG + chr5.3:28544251-28544270 MS.gene070578:CDS 50.0%
! CGGAGTTAGAGTTTTGCCAG+AGG - chr5.3:28542876-28542895 None:intergenic 50.0%
! GAGACGGCTTTTCAACTTGG+AGG - chr5.3:28542970-28542989 None:intergenic 50.0%
! TCTAGAGCGAGTCAGTCCTT+GGG + chr5.3:28544050-28544069 MS.gene070578:CDS 50.0%
!! TTCTAGAGCGAGTCAGTCCT+TGG + chr5.3:28544049-28544068 MS.gene070578:CDS 50.0%
AAAATCTGGCAGTGCTGCGC+CGG + chr5.3:28542575-28542594 MS.gene070578:CDS 55.0%
ACTGCCGTAACGATCTCCGT+TGG + chr5.3:28543166-28543185 MS.gene070578:CDS 55.0%
AGCACACTCTCCCTTCTCCA+TGG + chr5.3:28542686-28542705 MS.gene070578:CDS 55.0%
AGCATGCGCCATGGACGATT+AGG - chr5.3:28543462-28543481 None:intergenic 55.0%
AGTGTGCTAGGGTTTGGGGA+TGG - chr5.3:28542674-28542693 None:intergenic 55.0%
ATGGACGGGAACCATGGAGA+AGG - chr5.3:28542700-28542719 None:intergenic 55.0%
CAACTTGGAGGTCAAGTGGC+GGG - chr5.3:28542958-28542977 None:intergenic 55.0%
CGGTGCAGGGAATGAATGGA+CGG - chr5.3:28542715-28542734 None:intergenic 55.0%
CTCCGCCAATTCTACAGCCT+CGG - chr5.3:28543245-28543264 None:intergenic 55.0%
GAGACGGTGCAGGGAATGAA+TGG - chr5.3:28542719-28542738 None:intergenic 55.0%
GAGTAGATCAACCGCGGAGA+CGG - chr5.3:28542735-28542754 None:intergenic 55.0%
GGTGCAGGGAATGAATGGAC+GGG - chr5.3:28542714-28542733 None:intergenic 55.0%
GTCAGCGAGTAGATCAACCG+CGG - chr5.3:28542741-28542760 None:intergenic 55.0%
GTGGACACGGTCGTTAGTGA+CGG - chr5.3:28542804-28542823 None:intergenic 55.0%
TCGTCTTCACGAACCTCAGG+AGG - chr5.3:28542830-28542849 None:intergenic 55.0%
TGGACACGGTCGTTAGTGAC+GGG - chr5.3:28542803-28542822 None:intergenic 55.0%
TGGACGGGAACCATGGAGAA+GGG - chr5.3:28542699-28542718 None:intergenic 55.0%
TGGCAGCACCAAACTTCTGC+CGG - chr5.3:28543198-28543217 None:intergenic 55.0%
! AGGGAGAGTGTGCTAGGGTT+TGG - chr5.3:28542680-28542699 None:intergenic 55.0%
! AGTGGTGATCAGGTGTCGCA+AGG - chr5.3:28543059-28543078 None:intergenic 55.0%
! GCGCAGCACTGCCAGATTTT+TGG - chr5.3:28542575-28542594 None:intergenic 55.0%
! GGAGAAGGGAGAGTGTGCTA+GGG - chr5.3:28542685-28542704 None:intergenic 55.0%
! GGAGAGTGTGCTAGGGTTTG+GGG - chr5.3:28542678-28542697 None:intergenic 55.0%
! GGGAGAGTGTGCTAGGGTTT+GGG - chr5.3:28542679-28542698 None:intergenic 55.0%
! GGGGTTGAGGTTGTTTCCTC+TGG + chr5.3:28542857-28542876 MS.gene070578:CDS 55.0%
! GGTCGTTAGTGACGGGAGTT+AGG - chr5.3:28542796-28542815 None:intergenic 55.0%
! GTGGTGATCAGGTGTCGCAA+GGG - chr5.3:28543058-28543077 None:intergenic 55.0%
! TGGAGAAGGGAGAGTGTGCT+AGG - chr5.3:28542686-28542705 None:intergenic 55.0%
!! AGACGAAGATGACGGGGTTG+AGG + chr5.3:28542844-28542863 MS.gene070578:CDS 55.0%
AAATCTGGCAGTGCTGCGCC+GGG + chr5.3:28542576-28542595 MS.gene070578:CDS 60.0%
AAGCTTCGCCACGTCTACCG+TGG + chr5.3:28543028-28543047 MS.gene070578:CDS 60.0%
AGAGCGAGTCAGTCCTTGGG+AGG + chr5.3:28544053-28544072 MS.gene070578:CDS 60.0%
AGCTTCGCCACGTCTACCGT+GGG + chr5.3:28543029-28543048 MS.gene070578:CDS 60.0%
CACGAACCTCAGGAGGTTCG+TGG - chr5.3:28542823-28542842 None:intergenic 60.0%
CAGCACCAAACTTCTGCCGG+CGG - chr5.3:28543195-28543214 None:intergenic 60.0%
CCTCAGGAGGTTCGTGGACA+CGG - chr5.3:28542817-28542836 None:intergenic 60.0%
CGATCTCCGTTGGAATCCGC+CGG + chr5.3:28543176-28543195 MS.gene070578:CDS 60.0%
GGAATCCGCCGGCAGAAGTT+TGG + chr5.3:28543187-28543206 MS.gene070578:CDS 60.0%
TCGATGACATGCAGGGAGCC+AGG + chr5.3:28544259-28544278 MS.gene070578:CDS 60.0%
TCGTCCATGGCGCATGCTTG+AGG + chr5.3:28543464-28543483 MS.gene070578:CDS 60.0%
! CTTCTGCCGGCGGATTCCAA+CGG - chr5.3:28543185-28543204 None:intergenic 60.0%
!! GACTCGCTCTAGAACCTGCG+AGG - chr5.3:28544043-28544062 None:intergenic 60.0%
AAGGGGGAGGATGGCCGTAG+AGG + chr5.3:28544405-28544424 MS.gene070578:CDS 65.0%
AGGGAGTCCCACGGTAGACG+TGG - chr5.3:28543039-28543058 None:intergenic 65.0%
ATCAACCGCGGAGACGGTGC+AGG - chr5.3:28542729-28542748 None:intergenic 65.0%
CATTCCCTGCACCGTCTCCG+CGG + chr5.3:28542721-28542740 MS.gene070578:CDS 65.0%
CCGTGTCCACGAACCTCCTG+AGG + chr5.3:28542814-28542833 MS.gene070578:CDS 65.0%
TCAACCGCGGAGACGGTGCA+GGG - chr5.3:28542728-28542747 None:intergenic 65.0%
! GGTGTCGCAAGGGAGTCCCA+CGG - chr5.3:28543048-28543067 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.3 gene 28542542 28544452 28542542 ID=MS.gene070578
chr5.3 mRNA 28542542 28544452 28542542 ID=MS.gene070578.t1;Parent=MS.gene070578
chr5.3 exon 28542542 28543485 28542542 ID=MS.gene070578.t1.exon1;Parent=MS.gene070578.t1
chr5.3 CDS 28542542 28543485 28542542 ID=cds.MS.gene070578.t1;Parent=MS.gene070578.t1
chr5.3 exon 28543519 28543522 28543519 ID=MS.gene070578.t1.exon2;Parent=MS.gene070578.t1
chr5.3 CDS 28543519 28543522 28543519 ID=cds.MS.gene070578.t1;Parent=MS.gene070578.t1
chr5.3 exon 28544018 28544452 28544018 ID=MS.gene070578.t1.exon3;Parent=MS.gene070578.t1
chr5.3 CDS 28544018 28544452 28544018 ID=cds.MS.gene070578.t1;Parent=MS.gene070578.t1
Gene Sequence

>MS.gene070578

ATGTCTCCTCAGCAACCTAACCGTATTGATCCAAAAATCTGGCAGTGCTGCGCCGGGTCTGCCTTTAAAATTCCAAAACTACATTCTCATGTTTACTACTTCCCATTAGGGCACTTAGAACACATATGTCCATCCCCAAACCCTAGCACACTCTCCCTTCTCCATGGTTCCCGTCCATTCATTCCCTGCACCGTCTCCGCGGTTGATCTACTCGCTGACTCTCGTACCGACGAAGTCTTCGTCAAACTACTCCTAACTCCCGTCACTAACGACCGTGTCCACGAACCTCCTGAGGTTCGTGAAGACGAAGATGACGGGGTTGAGGTTGTTTCCTCTGGCAAAACTCTAACTCCGTCTGATGCTAATAACGGTGGTGCATTCTCCGTACCTAGCGAATGCGCAAGATTAATCTTCCCGCCACTTGACCTCCAAGTTGAAAAGCCGTCTCAAGAACTCTCAGTAAAGGACATTCACGGCAAAGAATGGAAGCTTCGCCACGTCTACCGTGGGACTCCCTTGCGACACCTGATCACCACTAACTGGAGTGAGTTTGTTGACGAGAAGAAACTGATTGGTGGTGATTCCTTAGTTTTCATGAAAAAATCAGCTGGAACTGGAACTGAAACTGCCGTAACGATCTCCGTTGGAATCCGCCGGCAGAAGTTTGGTGCTGCCACTAAGATAACAGAAAAATCGGTTACCGAGGCTGTAGAATTGGCGGAGAAGAACATGGCTTTCGAAGTTGTGTATTATCCTACTGCTGAAGTTTGGTGTGATTTTGTAGTTAATGCTAAGGTTGTGGAGGAAGCCATTAAGATTAAATGGAATCCTGGATTGAGAATCAAACTTTCTTTGAAGAAAGACAATTCTCAAAGAGATGCTCAAAATTTGAGGGAACAATATCTACTCTATCTGCTCCTAATCGTCCATGGCGCATGCTTGAGGTATATATTTATATATGCTCTCTCTTCTTTTAGTCTTGTTATATTTCAACTGCACAATGTTATTATATGTAGTATTTTTGTTTAATAAATGTTATCGGTCAAAGTTATGGTTCATGTTAGGAACTATATTTTTTTGATCAGTTCATGTTAGGAACTATATGTTCCAATGTGAAAAATATAGGATATGTTCCAATGTGTTAATCTTAAACATTAATATTGTGCAATTAAATTTAAGTGGCTTAATTCCTTTTTTGAGTCATATCGTACCACATCATGAATTAACTATAATGACTTTAGAAACTTTATACTTGAAATAGGTCGAATAAGTTTTTTTTTCTTTTTTTGGGCAAATGGTCGAATAAGTTGTCTCACTTGTGTGTTTACATATGTATGTACAATATACTTTATTGGATTAAATAAGTTTCTCGTCCCTGTAAATATAACGAATTTTGATTTTAATCTCTAAAAAATAAAATGAGATGTTTTAAATGATTGATACGGATACTTATCATTTTTCATGTCAATATCATAGGTTAATTGGGATGAACATAAAGCCTCGCAGGTTCTAGAGCGAGTCAGTCCTTGGGAGGTTGAGCTTATTTCCAACATACATGCACTGCATCCACAGTTCCCTCGAACAAAAAAGCTTAGGACTGCTAAAGATTCTGCCTTACGCAATGATATAGAGGGAGACTCTTCCATCCCAAAGATAGAGTTTCTTTCTTCAGCGGAAAGTTCCTTCAATGAAACATCGTTGAATAGCAATGCTTTGCTCGATGACATGCAGGGAGCCAGGCACAATCTTTTTTCCGCATCAACTTCTTCCAACTGGAACACCAAAAAATTTAGTCCTGGTTCAATTATGTTATTTGGTCAGATCATACGTCCTGTTGGAAGTGATTTACACGATTCAGATATCAAGGGGGAGGATGGCCGTAGAGGATGCAATGAAAATAGTCTTGTGTAG
Protein sequence

>MS.gene070578.t1

MSPQQPNRIDPKIWQCCAGSAFKIPKLHSHVYYFPLGHLEHICPSPNPSTLSLLHGSRPFIPCTVSAVDLLADSRTDEVFVKLLLTPVTNDRVHEPPEVREDEDDGVEVVSSGKTLTPSDANNGGAFSVPSECARLIFPPLDLQVEKPSQELSVKDIHGKEWKLRHVYRGTPLRHLITTNWSEFVDEKKLIGGDSLVFMKKSAGTGTETAVTISVGIRRQKFGAATKITEKSVTEAVELAEKNMAFEVVYYPTAEVWCDFVVNAKVVEEAIKIKWNPGLRIKLSLKKDNSQRDAQNLREQYLLYLLLIVHGACLSLVNWDEHKASQVLERVSPWEVELISNIHALHPQFPRTKKLRTAKDSALRNDIEGDSSIPKIEFLSSAESSFNETSLNSNALLDDMQGARHNLFSASTSSNWNTKKFSPGSIMLFGQIIRPVGSDLHDSDIKGEDGRRGCNENSLV