AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene070773

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070773.t1 MTR_4g049790 72.381 630 107 6 1 630 1 563 0.0 813
MS.gene070773.t1 MTR_4g047580 67.164 603 135 9 28 630 1 540 0.0 724
MS.gene070773.t1 MTR_4g049760 69.596 569 119 6 33 601 8 522 0.0 694
MS.gene070773.t1 MTR_2g088000 44.612 464 173 14 206 607 150 591 1.40e-93 301
MS.gene070773.t1 MTR_4g132430 57.018 228 84 6 345 566 105 324 7.32e-73 239
MS.gene070773.t1 MTR_4g132430 57.018 228 84 6 345 566 239 458 5.45e-72 241
MS.gene070773.t1 MTR_2g023930 45.378 357 135 10 265 579 192 530 3.84e-70 238
MS.gene070773.t1 MTR_3g053330 45.344 247 100 5 343 578 222 444 4.29e-63 216
MS.gene070773.t1 MTR_7g110720 46.640 253 101 6 352 603 174 393 1.29e-56 197
MS.gene070773.t1 MTR_7g110720 46.640 253 101 6 352 603 201 420 1.66e-56 197
MS.gene070773.t1 MTR_2g045360 49.020 204 89 5 352 543 196 396 6.92e-48 174
MS.gene070773.t1 MTR_7g009730 51.613 124 54 1 324 441 170 293 7.09e-37 146
MS.gene070773.t1 MTR_4g094412 37.453 267 135 6 352 593 228 487 1.70e-35 140
MS.gene070773.t1 MTR_4g094412 37.453 267 135 6 352 593 219 478 2.78e-35 139
MS.gene070773.t1 MTR_4g107970 53.020 149 57 3 352 487 163 311 4.96e-33 134
MS.gene070773.t1 MTR_4g107970 53.020 149 57 3 352 487 240 388 7.66e-33 134
MS.gene070773.t1 MTR_3g056100 44.037 109 57 2 340 445 231 338 2.99e-24 106
MS.gene070773.t1 MTR_3g056100 44.037 109 57 2 340 445 244 351 3.22e-24 106
MS.gene070773.t1 MTR_5g094430 55.000 80 35 1 350 429 213 291 4.42e-24 105
MS.gene070773.t1 MTR_3g056100 44.037 109 57 2 340 445 375 482 4.46e-24 107
MS.gene070773.t1 MTR_3g056100 44.037 109 57 2 340 445 388 495 4.62e-24 107
MS.gene070773.t1 MTR_5g094430 55.000 80 35 1 350 429 235 313 5.01e-24 105
MS.gene070773.t1 MTR_7g011820 49.462 93 46 1 347 439 390 481 5.61e-24 106
MS.gene070773.t1 MTR_5g042920 54.321 81 36 1 349 429 302 381 9.01e-24 105
MS.gene070773.t1 MTR_5g042920 36.111 108 65 3 368 475 152 255 3.49e-11 66.2
MS.gene070773.t1 MTR_5g094430 55.000 80 35 1 350 429 347 425 1.38e-23 105
MS.gene070773.t1 MTR_4g007060 35.099 151 74 4 344 494 105 231 2.59e-23 100
MS.gene070773.t1 MTR_4g007060 35.099 151 74 4 344 494 139 265 4.18e-23 100
MS.gene070773.t1 MTR_4g082580 51.852 81 38 1 349 429 260 339 9.95e-23 101
MS.gene070773.t1 MTR_5g074400 48.387 93 47 1 337 429 390 481 1.40e-22 102
MS.gene070773.t1 MTR_3g009470 54.321 81 36 1 349 429 477 556 1.82e-22 102
MS.gene070773.t1 MTR_3g009470 43.750 64 34 2 368 431 281 342 4.81e-11 66.2
MS.gene070773.t1 MTR_3g031220 46.316 95 50 1 334 428 332 425 4.38e-22 100
MS.gene070773.t1 MTR_3g031220 40.000 90 45 4 342 431 180 260 3.67e-12 69.7
MS.gene070773.t1 MTR_7g071120 54.321 81 36 1 349 429 306 385 6.73e-22 100
MS.gene070773.t1 MTR_4g013260 53.165 79 36 1 349 427 318 395 1.13e-21 99.4
MS.gene070773.t1 MTR_4g013260 41.860 86 43 3 351 434 170 250 8.13e-12 68.6
MS.gene070773.t1 MTR_5g044695 67.925 53 17 0 345 397 55 107 1.43e-21 90.5
MS.gene070773.t1 MTR_5g097490 53.012 83 37 2 348 429 232 313 2.76e-21 97.1
MS.gene070773.t1 MTR_7g080000 53.947 76 34 1 354 429 419 493 3.74e-21 98.6
MS.gene070773.t1 MTR_7g109800 45.745 94 46 2 352 445 130 218 4.37e-21 94.7
MS.gene070773.t1 MTR_3g106060 41.837 98 56 1 328 424 98 195 4.86e-21 94.4
MS.gene070773.t1 MTR_7g080020 50.617 81 39 1 349 429 475 554 5.31e-21 98.2
MS.gene070773.t1 MTR_4g094588 41.667 108 62 1 354 461 165 271 9.37e-21 94.4
MS.gene070773.t1 MTR_1g013790 36.290 124 64 4 318 427 39 161 1.56e-20 92.0
MS.gene070773.t1 MTR_6g038890 67.925 53 17 0 345 397 166 218 1.62e-20 90.9
MS.gene070773.t1 MTR_2g105060 48.718 78 38 1 353 428 153 230 2.67e-20 92.8
MS.gene070773.t1 MTR_2g105060 48.718 78 38 1 353 428 78 155 2.77e-20 90.9
MS.gene070773.t1 MTR_1g013760 44.565 92 51 0 352 443 125 216 4.42e-20 91.3
MS.gene070773.t1 MTR_1g009613 56.716 67 29 0 368 434 263 329 1.83e-19 90.5
MS.gene070773.t1 MTR_8g096730 37.736 106 65 1 325 430 28 132 1.93e-19 85.9
MS.gene070773.t1 MTR_2g033820 48.718 78 39 1 354 431 190 266 2.57e-19 90.9
MS.gene070773.t1 MTR_7g100510 42.857 98 45 2 330 427 334 420 2.69e-19 91.3
MS.gene070773.t1 MTR_7g100510 44.444 72 38 2 368 439 190 259 1.75e-11 67.0
MS.gene070773.t1 MTR_1g015140 51.351 74 35 1 352 425 87 159 3.36e-19 85.5
MS.gene070773.t1 MTR_7g079010 45.263 95 48 2 354 448 128 218 3.81e-19 87.0
MS.gene070773.t1 MTR_4g094908 56.452 62 27 0 368 429 267 328 5.40e-19 89.4
MS.gene070773.t1 MTR_1g086790 44.444 90 45 2 354 443 145 229 1.15e-18 88.2
MS.gene070773.t1 MTR_3g104750 39.252 107 57 2 319 425 63 161 1.22e-18 84.0
MS.gene070773.t1 MTR_8g087000 53.968 63 29 0 367 429 77 139 2.03e-18 85.5
MS.gene070773.t1 MTR_7g028415 52.857 70 32 1 357 426 83 151 2.05e-18 83.6
MS.gene070773.t1 MTR_7g028710 52.857 70 32 1 357 426 83 151 2.05e-18 83.6
MS.gene070773.t1 MTR_8g087000 53.968 63 29 0 367 429 76 138 2.07e-18 85.5
MS.gene070773.t1 MTR_5g016610 56.667 60 26 0 367 426 199 258 2.29e-18 85.9
MS.gene070773.t1 MTR_2g024350 34.043 141 81 3 362 491 3 142 2.83e-18 84.7
MS.gene070773.t1 MTR_2g032030 31.792 173 98 5 284 437 166 337 2.95e-18 88.6
MS.gene070773.t1 MTR_2g032030 31.792 173 98 5 284 437 166 337 2.96e-18 88.2
MS.gene070773.t1 MTR_7g109600 42.857 91 49 1 363 453 49 136 2.99e-18 85.5
MS.gene070773.t1 MTR_1g070455 57.895 57 24 0 368 424 294 350 7.69e-18 86.3
MS.gene070773.t1 MTR_4g130900 35.669 157 82 4 292 431 268 422 1.17e-17 86.7
MS.gene070773.t1 MTR_5g091390 47.436 78 40 1 354 431 144 220 1.18e-17 84.7
MS.gene070773.t1 MTR_2g083870 45.570 79 43 0 358 436 71 149 1.80e-17 83.2
MS.gene070773.t1 MTR_2g016770 51.562 64 31 0 364 427 32 95 2.82e-17 81.6
MS.gene070773.t1 MTR_8g092010 34.559 136 82 5 298 430 39 170 3.92e-17 80.1
MS.gene070773.t1 MTR_2g016770 51.562 64 31 0 364 427 24 87 4.11e-17 80.9
MS.gene070773.t1 MTR_7g079040 48.000 75 39 0 363 437 150 224 5.89e-17 82.8
MS.gene070773.t1 MTR_8g007270 48.101 79 40 1 346 424 133 210 7.31e-17 80.5
MS.gene070773.t1 MTR_4g122530 46.053 76 40 1 353 428 194 268 8.24e-17 83.6
MS.gene070773.t1 MTR_2g024380 44.186 86 46 1 353 438 239 322 1.27e-16 83.6
MS.gene070773.t1 MTR_8g028565 46.667 75 40 0 363 437 151 225 2.49e-16 81.3
MS.gene070773.t1 MTR_3g095080 42.391 92 51 2 354 445 131 220 3.67e-16 78.6
MS.gene070773.t1 MTR_4g127040 53.448 58 27 0 367 424 245 302 4.27e-16 80.1
MS.gene070773.t1 MTR_8g098945 44.444 72 40 0 366 437 128 199 4.68e-16 79.3
MS.gene070773.t1 MTR_3g095080 42.391 92 51 2 354 445 155 244 5.41e-16 78.6
MS.gene070773.t1 MTR_5g043880 47.436 78 40 1 347 424 104 180 5.70e-16 79.0
MS.gene070773.t1 MTR_1g099600 40.860 93 53 1 365 457 183 273 2.35e-15 77.8
MS.gene070773.t1 MTR_1g077780 46.053 76 40 1 354 429 49 123 3.95e-15 73.6
MS.gene070773.t1 MTR_3g095040 46.667 75 39 1 354 428 105 178 5.68e-15 74.7
MS.gene070773.t1 MTR_8g092140 48.611 72 35 2 354 424 146 216 1.26e-14 74.3
MS.gene070773.t1 MTR_1g078260 48.611 72 35 2 354 424 234 304 6.09e-14 75.1
MS.gene070773.t1 MTR_6g086555 47.500 80 40 2 354 432 193 271 9.75e-14 73.9
MS.gene070773.t1 MTR_3g108960 42.029 69 39 1 356 424 304 371 1.24e-12 70.5
MS.gene070773.t1 MTR_3g031220 40.000 90 45 4 342 431 180 260 1.43e-12 70.1
MS.gene070773.t1 MTR_4g007060 40.984 61 36 0 344 404 139 199 4.58e-12 67.0
MS.gene070773.t1 MTR_0117s0040 42.029 69 39 1 356 424 64 131 5.32e-12 66.6
MS.gene070773.t1 MTR_3g009470 43.750 64 34 2 368 431 281 342 2.63e-11 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene070773.t1 AT4G04450 41.463 492 197 15 105 578 85 503 2.66e-84 275
MS.gene070773.t1 AT4G04450 41.079 482 193 15 115 578 1 409 9.94e-81 262
MS.gene070773.t1 AT1G62300 50.923 271 109 9 343 594 287 552 2.05e-67 230
MS.gene070773.t1 AT4G22070 57.209 215 70 4 346 547 276 481 2.35e-61 214
MS.gene070773.t1 AT4G22070 57.209 215 70 4 346 547 276 481 2.35e-61 214
MS.gene070773.t1 AT4G22070 57.209 215 70 4 346 547 276 481 2.35e-61 214
MS.gene070773.t1 AT4G22070 57.209 215 70 4 346 547 276 481 2.35e-61 214
MS.gene070773.t1 AT4G01720 42.712 295 128 8 301 575 170 443 3.27e-46 171
MS.gene070773.t1 AT5G15130 36.900 271 120 7 347 579 165 422 7.90e-39 150
MS.gene070773.t1 AT5G15130 36.900 271 120 7 347 579 207 464 8.07e-39 151
MS.gene070773.t1 AT1G68150 62.887 97 36 0 352 448 220 316 1.09e-38 147
MS.gene070773.t1 AT1G69810 45.455 176 85 5 321 495 103 268 6.36e-36 138
MS.gene070773.t1 AT1G69810 45.455 176 85 5 321 495 161 326 7.74e-36 139
MS.gene070773.t1 AT1G18860 30.709 381 207 10 140 505 48 386 1.78e-34 138
MS.gene070773.t1 AT2G03340 44.828 116 54 2 314 429 371 476 3.19e-24 107
MS.gene070773.t1 AT2G03340 51.667 60 27 2 368 427 251 308 1.24e-11 68.2
MS.gene070773.t1 AT1G13960 55.696 79 34 1 349 427 238 315 6.15e-24 104
MS.gene070773.t1 AT1G13960 47.222 72 36 2 368 439 77 146 7.28e-12 67.8
MS.gene070773.t1 AT4G26640 52.500 80 37 1 350 429 188 266 7.00e-24 104
MS.gene070773.t1 AT4G26640 52.500 80 37 1 350 429 188 266 7.00e-24 104
MS.gene070773.t1 AT4G26640 52.500 80 37 1 350 429 188 266 7.00e-24 104
MS.gene070773.t1 AT4G26640 52.500 80 37 1 350 429 188 266 7.00e-24 104
MS.gene070773.t1 AT1G13960 55.696 79 34 1 349 427 391 468 8.26e-24 106
MS.gene070773.t1 AT1G13960 47.222 72 36 2 368 439 230 299 5.93e-12 68.9
MS.gene070773.t1 AT2G38470 54.321 81 36 1 349 429 344 423 1.60e-23 105
MS.gene070773.t1 AT2G38470 37.363 91 54 3 337 427 155 242 8.04e-11 65.5
MS.gene070773.t1 AT4G26640 52.500 80 37 1 350 429 324 402 1.96e-23 105
MS.gene070773.t1 AT4G26640 52.500 80 37 1 350 429 292 370 2.08e-23 104
MS.gene070773.t1 AT4G26640 52.500 80 37 1 350 429 364 442 2.80e-23 104
MS.gene070773.t1 AT4G31800 43.810 105 54 1 346 445 85 189 2.83e-22 97.1
MS.gene070773.t1 AT4G31800 43.810 105 54 1 346 445 150 254 4.32e-22 97.8
MS.gene070773.t1 AT2G04880 45.055 91 49 1 337 427 253 342 7.05e-22 99.8
MS.gene070773.t1 AT2G04880 49.275 69 31 3 363 430 107 172 8.71e-12 68.2
MS.gene070773.t1 AT5G56270 53.086 81 37 1 349 429 469 548 8.11e-22 100
MS.gene070773.t1 AT2G04880 45.055 91 49 1 337 427 277 366 9.25e-22 99.8
MS.gene070773.t1 AT2G04880 49.275 69 31 3 363 430 107 172 9.64e-12 68.2
MS.gene070773.t1 AT3G01080 51.899 79 37 1 349 427 288 365 2.61e-21 97.4
MS.gene070773.t1 AT3G01080 51.899 79 37 1 349 427 326 403 2.69e-21 97.8
MS.gene070773.t1 AT2G25000 32.584 178 103 4 267 430 14 188 3.05e-21 94.0
MS.gene070773.t1 AT1G29860 46.809 94 45 2 352 445 121 209 4.36e-21 94.4
MS.gene070773.t1 AT5G07100 48.235 85 43 1 347 431 121 204 5.10e-21 92.4
MS.gene070773.t1 AT5G07100 48.235 85 43 1 347 431 121 204 5.10e-21 92.4
MS.gene070773.t1 AT5G07100 48.235 85 43 1 347 431 121 204 5.10e-21 92.4
MS.gene070773.t1 AT5G07100 48.235 85 43 1 347 431 121 204 5.10e-21 92.4
MS.gene070773.t1 AT1G80840 46.154 78 40 1 353 428 130 207 7.36e-21 94.0
MS.gene070773.t1 AT5G07100 48.235 85 43 1 347 431 214 297 9.95e-21 94.0
MS.gene070773.t1 AT5G41570 45.455 99 49 2 354 452 85 178 1.38e-20 90.1
MS.gene070773.t1 AT4G18170 44.000 100 55 1 354 453 159 257 1.68e-20 93.6
MS.gene070773.t1 AT1G64000 53.247 77 35 1 349 425 96 171 1.75e-20 90.5
MS.gene070773.t1 AT2G25000 34.211 152 94 3 282 430 18 166 1.88e-20 91.3
MS.gene070773.t1 AT2G25000 34.211 152 94 3 282 430 18 166 1.88e-20 91.3
MS.gene070773.t1 AT2G25000 34.211 152 94 3 282 430 61 209 3.65e-20 91.3
MS.gene070773.t1 AT5G26170 52.778 72 33 1 353 424 99 169 4.46e-20 88.6
MS.gene070773.t1 AT5G46350 54.054 74 33 1 354 427 170 242 1.09e-19 91.3
MS.gene070773.t1 AT2G30250 46.512 86 45 1 346 431 307 391 1.68e-19 91.7
MS.gene070773.t1 AT1G29280 51.429 70 34 0 358 427 65 134 1.76e-19 89.0
MS.gene070773.t1 AT2G23320 59.322 59 24 0 368 426 241 299 1.82e-19 90.5
MS.gene070773.t1 AT5G49520 46.250 80 42 1 352 431 206 284 2.78e-19 90.9
MS.gene070773.t1 AT4G30935 40.000 125 71 3 335 457 299 421 4.92e-19 90.9
MS.gene070773.t1 AT2G30590 57.812 64 26 1 368 430 314 377 6.19e-19 89.7
MS.gene070773.t1 AT5G13080 52.055 73 34 1 354 426 54 125 1.09e-18 83.6
MS.gene070773.t1 AT1G55600 48.684 76 38 1 354 429 294 368 1.73e-18 89.4
MS.gene070773.t1 AT4G26440 51.316 76 36 1 354 429 359 433 2.63e-18 89.4
MS.gene070773.t1 AT3G58710 46.575 73 39 0 358 430 62 134 5.81e-18 85.1
MS.gene070773.t1 AT3G58710 46.575 73 39 0 358 430 61 133 6.22e-18 84.7
MS.gene070773.t1 AT5G28650 60.377 53 21 0 372 424 267 319 6.82e-18 85.9
MS.gene070773.t1 AT4G24240 56.667 60 26 0 367 426 281 340 8.03e-18 85.9
MS.gene070773.t1 AT2G46130 50.000 72 35 1 354 425 17 87 1.16e-17 79.3
MS.gene070773.t1 AT3G01970 51.389 72 34 1 353 424 51 121 1.27e-17 80.5
MS.gene070773.t1 AT3G62340 52.703 74 34 1 354 427 105 177 1.47e-17 84.0
MS.gene070773.t1 AT3G04670 57.895 57 24 0 368 424 263 319 1.57e-17 84.7
MS.gene070773.t1 AT2G24570 59.649 57 23 0 368 424 244 300 1.78e-17 84.3
MS.gene070773.t1 AT2G47260 45.783 83 44 1 352 434 159 240 1.89e-17 84.7
MS.gene070773.t1 AT5G64810 46.809 94 46 2 334 424 74 166 2.68e-17 81.3
MS.gene070773.t1 AT2G44745 39.286 112 63 3 316 424 92 201 3.02e-17 81.6
MS.gene070773.t1 AT5G52830 45.455 77 42 0 361 437 159 235 3.21e-17 84.0
MS.gene070773.t1 AT4G01250 46.429 84 43 1 354 437 117 198 3.75e-17 83.2
MS.gene070773.t1 AT4G31550 57.895 57 24 0 368 424 247 303 4.59e-17 83.2
MS.gene070773.t1 AT4G31550 57.895 57 24 0 368 424 246 302 4.62e-17 83.2
MS.gene070773.t1 AT2G34830 43.011 93 46 1 349 434 190 282 4.87e-17 84.3
MS.gene070773.t1 AT1G30650 43.011 93 46 1 349 434 192 284 5.24e-17 84.3
MS.gene070773.t1 AT2G34830 43.011 93 46 1 349 434 199 291 6.12e-17 84.3
MS.gene070773.t1 AT4G23550 44.872 78 43 0 362 439 135 212 2.46e-16 80.9
MS.gene070773.t1 AT5G45050 25.926 243 161 6 295 530 1082 1312 6.97e-16 82.4
MS.gene070773.t1 AT5G45050 25.926 243 161 6 295 530 971 1201 7.44e-16 82.4
MS.gene070773.t1 AT5G45050 25.926 243 161 6 295 530 1110 1340 8.12e-16 82.4
MS.gene070773.t1 AT4G39410 50.704 71 34 1 354 424 210 279 1.40e-15 78.6
MS.gene070773.t1 AT1G69310 44.000 75 41 1 350 424 130 203 1.70e-15 77.8
MS.gene070773.t1 AT1G69310 44.000 75 41 1 350 424 130 203 1.70e-15 77.8
MS.gene070773.t1 AT1G69310 44.000 75 41 1 350 424 130 203 1.70e-15 77.8
MS.gene070773.t1 AT2G37260 53.846 65 29 1 362 426 264 327 2.47e-15 78.6
MS.gene070773.t1 AT2G37260 48.529 68 28 3 368 430 86 151 2.24e-11 66.2
MS.gene070773.t1 AT2G37260 53.846 65 29 1 362 426 262 325 2.56e-15 78.2
MS.gene070773.t1 AT2G37260 48.529 68 28 3 368 430 84 149 2.28e-11 66.2
MS.gene070773.t1 AT2G37260 53.846 65 29 1 362 426 344 407 3.79e-15 78.6
MS.gene070773.t1 AT2G37260 48.529 68 28 3 368 430 166 231 2.56e-11 66.6
MS.gene070773.t1 AT2G46130 47.222 72 33 2 354 425 17 83 2.63e-13 67.0
MS.gene070773.t1 AT2G21900 42.029 69 39 1 362 430 104 171 3.06e-13 69.7
MS.gene070773.t1 AT5G45260 40.625 96 51 2 348 441 1193 1284 1.83e-12 71.2
MS.gene070773.t1 AT4G31800 48.438 64 28 1 346 404 150 213 7.07e-12 66.2

Find 115 sgRNAs with CRISPR-Local

Find 217 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTGTTACACCATCTTTAT+TGG 0.095497 4.4:+52430913 MS.gene070773:CDS
CCTAACTGATCTTGACTTCT+AGG 0.119265 4.4:-52432896 None:intergenic
CCAAAACTCTGAGGGATTAA+AGG 0.176833 4.4:-52434230 None:intergenic
GACAAAGCTGCAGGAATATT+TGG 0.214770 4.4:-52434407 None:intergenic
GTTCTCTTCTCTTCTGAATT+AGG 0.215204 4.4:-52431182 None:intergenic
GCTGCTATTACATCCATTAT+TGG 0.245842 4.4:+52434431 MS.gene070773:CDS
TAAAGTTTAGGTCTTGTAAT+TGG 0.259816 4.4:-52434622 None:intergenic
CCTTTAATCCCTCAGAGTTT+TGG 0.262834 4.4:+52434230 MS.gene070773:CDS
ACTGGGGCCAAGGAAATTTA+TGG 0.276997 4.4:+52432565 MS.gene070773:CDS
ACTGTTTGAGCAGTAATATT+TGG 0.280261 4.4:-52434362 None:intergenic
GGGAAAGAGTTGTTATTAAC+TGG 0.286280 4.4:-52430665 None:intergenic
GCGTTCCTTTGTGGAGTATT+TGG 0.301041 4.4:-52434191 None:intergenic
CGTAAGGGTAAGAAAGTTAA+AGG 0.311737 4.4:+52432485 MS.gene070773:CDS
GATAAGGTTAAGGAAGTTAA+AGG 0.318235 4.4:+52432725 MS.gene070773:CDS
AGAGCTTGAACAAACGAAAA+TGG 0.333537 4.4:+52432376 MS.gene070773:CDS
GATAAGGTTAAGGAAGTTAA+AGG 0.335137 4.4:+52432605 MS.gene070773:CDS
AGCTGACCCTTCACAAATAT+TGG 0.335185 4.4:+52434334 MS.gene070773:CDS
CCAAGGAAATTTATGGAACT+TGG 0.341755 4.4:+52432572 MS.gene070773:CDS
AGAAGAAACTTACATGCTTT+CGG 0.356423 4.4:-52433259 None:intergenic
GGATGTCAATGGAGAAAGTA+TGG 0.356478 4.4:+52433164 MS.gene070773:CDS
CTACTAGTATTGATAGTAAT+AGG 0.368547 4.4:-52431128 None:intergenic
AGAGAAGAGAACTAAGAGTA+TGG 0.375742 4.4:+52431193 MS.gene070773:CDS
AACTGAGAATGGTGGAGTAC+TGG 0.377375 4.4:+52432547 MS.gene070773:CDS
CTGCAGCGATAATAAGCTTT+AGG 0.381386 4.4:-52433215 None:intergenic
TCCCTTTCCTAGTCAATATA+AGG 0.384368 4.4:+52430756 MS.gene070773:CDS
AACTGATTTGCGTTCCTTTG+TGG 0.385254 4.4:-52434200 None:intergenic
TTTCAGATGGCTATTCTTCA+AGG 0.389126 4.4:+52432353 MS.gene070773:intron
AAAAGGAAATTTGATGATGC+TGG 0.391734 4.4:+52432692 MS.gene070773:CDS
GCTGCTGATAATAATAATAA+TGG 0.393320 4.4:+52434482 MS.gene070773:CDS
GCCATTTCTTTAGCTGCTGC+AGG 0.395885 4.4:-52433996 None:intergenic
TCAATTGTAATAGTTGGATA+TGG 0.399033 4.4:-52434155 None:intergenic
CTTTAGCTGCTGCAGGTAAA+GGG 0.402829 4.4:-52433989 None:intergenic
GAAATTTATGGAACTTGGAT+TGG 0.404712 4.4:+52432577 MS.gene070773:CDS
TCTTTAGCTGCTGCAGGTAA+AGG 0.405534 4.4:-52433990 None:intergenic
ACTTGGATTGGCTACTGATA+AGG 0.406023 4.4:+52432589 MS.gene070773:CDS
TAACATCCTCATTCTTACTT+AGG 0.406214 4.4:-52432961 None:intergenic
GCAGCAGCAGTGTTGTTGTT+TGG 0.408470 4.4:-52434464 None:intergenic
CTTTAACTTTCTTACCCTTA+CGG 0.409546 4.4:-52432484 None:intergenic
GCTACCATTGCTATTTCCTT+CGG 0.410299 4.4:-52434516 None:intergenic
TCAAGGTTGTTAGTATCATC+AGG 0.414586 4.4:-52430686 None:intergenic
TGATGGGAAGGAACGAAATC+AGG 0.420611 4.4:+52434595 MS.gene070773:CDS
CCAAGTTCCATAAATTTCCT+TGG 0.423079 4.4:-52432572 None:intergenic
GTGTAATCAATTGTAATAGT+TGG 0.425606 4.4:-52434161 None:intergenic
AACTTACATGCTTTCGGACT+GGG 0.430854 4.4:-52433253 None:intergenic
AAAATGAGAATTAAGAATGA+TGG 0.434196 4.4:+52432659 MS.gene070773:CDS
TGGCTGAGTATCACCAATAA+TGG 0.440113 4.4:-52434444 None:intergenic
ATTGGCTACTGATAAGGTTA+AGG 0.444001 4.4:+52432595 MS.gene070773:CDS
GTATGAATATGATCATTGTC+AGG 0.450321 4.4:-52430893 None:intergenic
ACCTGCAGCAGCTAAAGAAA+TGG 0.455287 4.4:+52433995 MS.gene070773:CDS
GTTTCATGAGATGATGAGAT+TGG 0.455627 4.4:-52430803 None:intergenic
ATTGGCTACTGATAAGGTTA+AGG 0.459989 4.4:+52432715 MS.gene070773:CDS
AGCTTTAGTTCCAATAAAGA+TGG 0.463299 4.4:-52430923 None:intergenic
GACCTTATATTGACTAGGAA+AGG 0.470626 4.4:-52430758 None:intergenic
ACTGAGAATGGTGGAGTACT+GGG 0.475263 4.4:+52432548 MS.gene070773:CDS
CCTAGAAGTCAAGATCAGTT+AGG 0.482042 4.4:+52432896 MS.gene070773:CDS
ACATGTGGCATCAACAATGA+TGG 0.482281 4.4:+52434578 MS.gene070773:CDS
GAAATTTGATGATGCTGGAT+TGG 0.483257 4.4:+52432697 MS.gene070773:CDS
ATATGATCATTGTCAGGGAC+AGG 0.484701 4.4:-52430887 None:intergenic
GCATCAACATCAAGCAATGA+TGG 0.494138 4.4:+52434056 MS.gene070773:CDS
GTTGGTGTCAAGGCAATGTA+AGG 0.496162 4.4:+52432805 MS.gene070773:CDS
TGCTGGATTGGCTACTGATA+AGG 0.503894 4.4:+52432709 MS.gene070773:CDS
GCTTTCGGACTGGGCAACCA+TGG 0.504496 4.4:-52433244 None:intergenic
ATGATGGAGAATTAATGAAA+AGG 0.504955 4.4:+52432675 MS.gene070773:CDS
AAACTTACATGCTTTCGGAC+TGG 0.512195 4.4:-52433254 None:intergenic
GAGAGTGATGCAAGACCGTA+AGG 0.513027 4.4:+52432469 MS.gene070773:CDS
GGTATTGCCAATATTTGTGA+AGG 0.514432 4.4:-52434341 None:intergenic
TCATGAAACAAGAACAGAGT+TGG 0.518840 4.4:+52430817 MS.gene070773:CDS
TAGTAGTGATCAATCTATGA+TGG 0.520634 4.4:+52431145 MS.gene070773:CDS
CGATAATAAGCTTTAGGACA+CGG 0.531100 4.4:-52433209 None:intergenic
ACCTTATATTGACTAGGAAA+GGG 0.537487 4.4:-52430757 None:intergenic
TTATTGAGTTAGATCACTGA+TGG 0.542017 4.4:+52433143 MS.gene070773:intron
TGGGACTAAGTTTCAACTCA+AGG 0.543159 4.4:-52430703 None:intergenic
GTTGTAATCACAACTTATGA+AGG 0.543545 4.4:+52433954 MS.gene070773:CDS
ATGGTGGAGTACTGGGGCCA+AGG 0.545172 4.4:+52432555 MS.gene070773:CDS
CTTTCGGACTGGGCAACCAT+GGG 0.546480 4.4:-52433243 None:intergenic
AGGAATTTCCAAAACTCTGA+GGG 0.547821 4.4:-52434238 None:intergenic
ACTTCAGAATGGTGGAGAGT+TGG 0.548067 4.4:+52432787 MS.gene070773:CDS
AGTATTGATAGTAATAGGAA+TGG 0.556477 4.4:-52431123 None:intergenic
AAAGGAAAAGAAGTGTTCAC+TGG 0.557236 4.4:+52432743 MS.gene070773:CDS
GTATTGATAGTAATAGGAAT+GGG 0.563883 4.4:-52431122 None:intergenic
TATCGCTGCAGCACGACCCA+TGG 0.564450 4.4:+52433227 MS.gene070773:CDS
ATAGCAGCTGACAAAGCTGC+AGG 0.574982 4.4:-52434416 None:intergenic
GAAGAGAACTAAGAGTATGG+AGG 0.575623 4.4:+52431196 MS.gene070773:CDS
GATAATAAGCTTTAGGACAC+GGG 0.575823 4.4:-52433208 None:intergenic
GATCAATCTATGATGGATGA+TGG 0.577380 4.4:+52431152 MS.gene070773:CDS
AGATCACTGATGGATGTCAA+TGG 0.579642 4.4:+52433153 MS.gene070773:intron
AGCAACAAAATTATCACATG+TGG 0.580366 4.4:+52434563 MS.gene070773:CDS
TAGCTGCTGCAGGTAAAGGG+TGG 0.582781 4.4:-52433986 None:intergenic
GTATTGCCAATATTTGTGAA+GGG 0.582929 4.4:-52434340 None:intergenic
CATGTGGCATCAACAATGAT+GGG 0.583795 4.4:+52434579 MS.gene070773:CDS
TACAAATGCTGATATTGTGA+TGG 0.589604 4.4:+52432841 MS.gene070773:CDS
GATAGTCCTAAGTAAGAATG+AGG 0.591251 4.4:+52432955 MS.gene070773:CDS
ATGGTGGAGAGTTGGTGTCA+AGG 0.595504 4.4:+52432795 MS.gene070773:CDS
AATATTTGGACTAGCAACAA+TGG 0.600303 4.4:-52434393 None:intergenic
TGGCATCAACAATGATGGGA+AGG 0.604261 4.4:+52434583 MS.gene070773:CDS
ATGAGATGATGAGATTGGAG+AGG 0.604597 4.4:-52430798 None:intergenic
ATTAACTGGAACTGAAGCCA+TGG 0.605747 4.4:-52430651 None:intergenic
TTGAGGGATCAGAGAAGCTG+AGG 0.609486 4.4:-52434258 None:intergenic
TATGAATATGATCATTGTCA+GGG 0.613441 4.4:-52430892 None:intergenic
GATGTCAATGGAGAAAGTAT+GGG 0.615340 4.4:+52433165 MS.gene070773:CDS
TGGAGGACCTTATATTGACT+AGG 0.616940 4.4:-52430763 None:intergenic
TTAAAACTTAACAGGTACAA+AGG 0.618245 4.4:+52433913 MS.gene070773:intron
AAGTCAAGATCAGTTAGGAT+CGG 0.620379 4.4:+52432901 MS.gene070773:CDS
TATGATCATTGTCAGGGACA+GGG 0.624825 4.4:-52430886 None:intergenic
CAAGGTTGTTAGTATCATCA+GGG 0.625070 4.4:-52430685 None:intergenic
GTCACCGAAGGAAATAGCAA+TGG 0.626521 4.4:+52434512 MS.gene070773:CDS
AATGGCAACAATGTCACCGA+AGG 0.626879 4.4:+52434500 MS.gene070773:CDS
GAGGAATTTCCAAAACTCTG+AGG 0.632072 4.4:-52434239 None:intergenic
AGCTAGATCAGAAGAAACCA+TGG 0.635656 4.4:+52433033 MS.gene070773:CDS
GGACAGAGACAACTTCATGA+TGG 0.651443 4.4:-52430865 None:intergenic
GAATAGATTACAATTACATG+CGG 0.660089 4.4:+52432435 MS.gene070773:CDS
AATCTCCAAATACTCCACAA+AGG 0.678231 4.4:+52434186 MS.gene070773:CDS
CTGAGAATGGTGGAGTACTG+GGG 0.687268 4.4:+52432549 MS.gene070773:CDS
GTTGCAATACTTGAAGAACA+AGG 0.694121 4.4:-52434116 None:intergenic
AGAGTGATGCAAGACCGTAA+GGG 0.707223 4.4:+52432470 MS.gene070773:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATAATCACATTAAAAA+CGG - chr4.4:52432317-52432336 None:intergenic 10.0%
!! ATATATATATATATGATTCT+TGG + chr4.4:52431488-52431507 MS.gene070773:intron 10.0%
!! GTAAAAAAAATAATAACAAT+GGG - chr4.4:52430975-52430994 None:intergenic 10.0%
!! TATAACATAATTATTATTTG+TGG - chr4.4:52431631-52431650 None:intergenic 10.0%
!!! ATTAATTCTTTTTAGTAAAA+AGG - chr4.4:52433121-52433140 None:intergenic 10.0%
!! AGCAAAAAAAATTAAAGTTT+AGG - chr4.4:52434637-52434656 None:intergenic 15.0%
!! CGTAAAAAAAATAATAACAA+TGG - chr4.4:52430976-52430995 None:intergenic 15.0%
!! TAATACAATTAAAACTTAAC+AGG + chr4.4:52433905-52433924 MS.gene070773:intron 15.0%
!! TAATTAAATTACTTATGAAG+TGG + chr4.4:52433861-52433880 MS.gene070773:intron 15.0%
!! TGTATTATACTTATAAATGT+AGG - chr4.4:52431555-52431574 None:intergenic 15.0%
!! TTTAGTAAATACATGTAATT+TGG - chr4.4:52433293-52433312 None:intergenic 15.0%
!!! TTTTGACTCAATAAAATTAA+AGG - chr4.4:52431286-52431305 None:intergenic 15.0%
!! AAAAAATTTAAAAGAAAGCG+AGG - chr4.4:52431424-52431443 None:intergenic 20.0%
!! AAAATGAGAATTAAGAATGA+TGG + chr4.4:52432659-52432678 MS.gene070773:CDS 20.0%
!! ATATATATATGATTCTTGGT+TGG + chr4.4:52431492-52431511 MS.gene070773:intron 20.0%
!! TAATTTCATTAAATTCTGCT+AGG - chr4.4:52431242-52431261 None:intergenic 20.0%
!!! AATTTTATTGAGTCAAAACT+GGG + chr4.4:52431288-52431307 MS.gene070773:intron 20.0%
!!! AATTTTGATTGGTTTTGATT+AGG - chr4.4:52434287-52434306 None:intergenic 20.0%
!!! ACATGCATATTTTAAAACAT+TGG + chr4.4:52433468-52433487 MS.gene070773:intron 20.0%
!!! TAATTTTATTGAGTCAAAAC+TGG + chr4.4:52431287-52431306 MS.gene070773:intron 20.0%
!!! TAATTTTTTTCACAAAGAAG+AGG - chr4.4:52432126-52432145 None:intergenic 20.0%
!!! TGTTAATTTTGATAATGTTG+AGG + chr4.4:52432979-52432998 MS.gene070773:CDS 20.0%
!!! TTCTACTATGTTATTCTTTT+TGG + chr4.4:52431442-52431461 MS.gene070773:intron 20.0%
! AAAATGTAACAGTTTATGTC+AGG - chr4.4:52431995-52432014 None:intergenic 25.0%
! AAAGGAAAAAAAGTCTTTGA+TGG + chr4.4:52432503-52432522 MS.gene070773:CDS 25.0%
! AACAATGGGATATTGAATTA+TGG - chr4.4:52430961-52430980 None:intergenic 25.0%
! ATTTAATTAGCGTACTATGT+CGG - chr4.4:52431819-52431838 None:intergenic 25.0%
! CAATAAACTCAAATCCAAAA+TGG + chr4.4:52433821-52433840 MS.gene070773:intron 25.0%
! CACTGACATACATATAAAAT+CGG - chr4.4:52432076-52432095 None:intergenic 25.0%
! CCAACAAAATCAAAATACAT+TGG - chr4.4:52432217-52432236 None:intergenic 25.0%
! GAATAGATTACAATTACATG+CGG + chr4.4:52432435-52432454 MS.gene070773:CDS 25.0%
! GCTGCTGATAATAATAATAA+TGG + chr4.4:52434482-52434501 MS.gene070773:CDS 25.0%
! GTCATATTCATTAATTAACG+TGG - chr4.4:52431723-52431742 None:intergenic 25.0%
! GTGTAATCAATTGTAATAGT+TGG - chr4.4:52434164-52434183 None:intergenic 25.0%
! TAAATACATAGATTGCTGAA+TGG - chr4.4:52433681-52433700 None:intergenic 25.0%
! TATGAATATGATCATTGTCA+GGG - chr4.4:52430895-52430914 None:intergenic 25.0%
! TCAAAATATGCAGTTATGTT+TGG + chr4.4:52433731-52433750 MS.gene070773:intron 25.0%
! TCAATTGTAATAGTTGGATA+TGG - chr4.4:52434158-52434177 None:intergenic 25.0%
! TTAAAACTTAACAGGTACAA+AGG + chr4.4:52433913-52433932 MS.gene070773:intron 25.0%
!! AATCACATTAAAAACGGATT+TGG - chr4.4:52432311-52432330 None:intergenic 25.0%
!! ACTAAAAATGTTTCTGTATG+TGG - chr4.4:52433543-52433562 None:intergenic 25.0%
!! ATGATGGAGAATTAATGAAA+AGG + chr4.4:52432675-52432694 MS.gene070773:CDS 25.0%
!! CAAAACCAATCAAAATTTTC+TGG + chr4.4:52434290-52434309 MS.gene070773:CDS 25.0%
!! GTATTGATAGTAATAGGAAT+GGG - chr4.4:52431125-52431144 None:intergenic 25.0%
!! TAAAGTTTAGGTCTTGTAAT+TGG - chr4.4:52434625-52434644 None:intergenic 25.0%
!! TATGTTTCTATGCTAGAAAA+TGG + chr4.4:52433642-52433661 MS.gene070773:intron 25.0%
!! TGTGATAATTTTGTTGCTAT+TGG - chr4.4:52434561-52434580 None:intergenic 25.0%
!! TTCATTGAACTATTTTCAGA+TGG + chr4.4:52432340-52432359 MS.gene070773:intron 25.0%
!!! AGTATTGATAGTAATAGGAA+TGG - chr4.4:52431126-52431145 None:intergenic 25.0%
!!! CCAATGTATTTTGATTTTGT+TGG + chr4.4:52432214-52432233 MS.gene070773:intron 25.0%
!!! CTACTAGTATTGATAGTAAT+AGG - chr4.4:52431131-52431150 None:intergenic 25.0%
!!! CTATGTTATTCTTTTTGGAA+AGG + chr4.4:52431447-52431466 MS.gene070773:intron 25.0%
AAGAAAAAGAGACTTCAGAA+TGG + chr4.4:52432776-52432795 MS.gene070773:CDS 30.0%
ACGTTCGAATAAAACATGTT+AGG - chr4.4:52432280-52432299 None:intergenic 30.0%
ACTGTTTGAGCAGTAATATT+TGG - chr4.4:52434365-52434384 None:intergenic 30.0%
AGAAGAAACTTACATGCTTT+CGG - chr4.4:52433262-52433281 None:intergenic 30.0%
AGCAACAAAATTATCACATG+TGG + chr4.4:52434563-52434582 MS.gene070773:CDS 30.0%
AGTTTATGTCAGGATCATAT+TGG - chr4.4:52431985-52432004 None:intergenic 30.0%
ATAATGCGAAAGCAATCAAT+AGG - chr4.4:52433334-52433353 None:intergenic 30.0%
CGTTCGAATAAAACATGTTA+GGG - chr4.4:52432279-52432298 None:intergenic 30.0%
GAAATTTATGGAACTTGGAT+TGG + chr4.4:52432577-52432596 MS.gene070773:CDS 30.0%
GATAAGGTTAAGGAAGTTAA+AGG + chr4.4:52432605-52432624 MS.gene070773:CDS 30.0%
GTATGAATATGATCATTGTC+AGG - chr4.4:52430896-52430915 None:intergenic 30.0%
GTATTGCCAATATTTGTGAA+GGG - chr4.4:52434343-52434362 None:intergenic 30.0%
GTTGTAATCACAACTTATGA+AGG + chr4.4:52433954-52433973 MS.gene070773:CDS 30.0%
TAACATCCTCATTCTTACTT+AGG - chr4.4:52432964-52432983 None:intergenic 30.0%
TAATGCGAAAGCAATCAATA+GGG - chr4.4:52433333-52433352 None:intergenic 30.0%
TACAAATGCTGATATTGTGA+TGG + chr4.4:52432841-52432860 MS.gene070773:CDS 30.0%
TACTGTTACACCATCTTTAT+TGG + chr4.4:52430913-52430932 MS.gene070773:CDS 30.0%
TAGAACACTGTTAACATGAT+GGG + chr4.4:52432189-52432208 MS.gene070773:intron 30.0%
TAGTAGTGATCAATCTATGA+TGG + chr4.4:52431145-52431164 MS.gene070773:CDS 30.0%
TTAGAACACTGTTAACATGA+TGG + chr4.4:52432188-52432207 MS.gene070773:intron 30.0%
TTATTGAGTTAGATCACTGA+TGG + chr4.4:52433143-52433162 MS.gene070773:intron 30.0%
! ACACTTTTACATACTTACCA+TGG - chr4.4:52433053-52433072 None:intergenic 30.0%
! ACCTTATATTGACTAGGAAA+GGG - chr4.4:52430760-52430779 None:intergenic 30.0%
! AGCTTTAGTTCCAATAAAGA+TGG - chr4.4:52430926-52430945 None:intergenic 30.0%
! CATGTTAACAGTGTTCTAAA+AGG - chr4.4:52432187-52432206 None:intergenic 30.0%
! CCTCAATACTCAAATTTTCA+GGG + chr4.4:52431947-52431966 MS.gene070773:intron 30.0%
! CTTTAACTTTCTTACCCTTA+CGG - chr4.4:52432487-52432506 None:intergenic 30.0%
! TATATGATTCTTGGTTGGTT+TGG + chr4.4:52431497-52431516 MS.gene070773:intron 30.0%
!! AAAAGGAAATTTGATGATGC+TGG + chr4.4:52432692-52432711 MS.gene070773:CDS 30.0%
!! AATATTTGGACTAGCAACAA+TGG - chr4.4:52434396-52434415 None:intergenic 30.0%
!! AGAAACATAAGCTTTGAAAG+TGG - chr4.4:52433631-52433650 None:intergenic 30.0%
!! CCTGAAAATTTGAGTATTGA+GGG - chr4.4:52431949-52431968 None:intergenic 30.0%
!! GTAGTCCAGAAAATTTTGAT+TGG - chr4.4:52434298-52434317 None:intergenic 30.0%
!!! CTGCATATTTTGATTTGCAA+TGG - chr4.4:52433724-52433743 None:intergenic 30.0%
!!! GGTTTTGATTAGGTATTTGA+GGG - chr4.4:52434277-52434296 None:intergenic 30.0%
!!! TGGTTTTGATTAGGTATTTG+AGG - chr4.4:52434278-52434297 None:intergenic 30.0%
AAAAAGAGAACTGAGAATGG+TGG + chr4.4:52432539-52432558 MS.gene070773:CDS 35.0%
AAAAAGAGACTTCAGAATGG+TGG + chr4.4:52432779-52432798 MS.gene070773:CDS 35.0%
AAAATGGTAGCTACTTAGCT+AGG + chr4.4:52433837-52433856 MS.gene070773:intron 35.0%
AAGTCAAGATCAGTTAGGAT+CGG + chr4.4:52432901-52432920 MS.gene070773:CDS 35.0%
AATCTCCAAATACTCCACAA+AGG + chr4.4:52434186-52434205 MS.gene070773:CDS 35.0%
ACCCACGTACACAAAAATAT+GGG + chr4.4:52431391-52431410 MS.gene070773:intron 35.0%
ACTTAGTCCCAACAAAAACA+TGG + chr4.4:52430715-52430734 MS.gene070773:CDS 35.0%
AGAACACTGTTAACATGATG+GGG + chr4.4:52432190-52432209 MS.gene070773:intron 35.0%
AGAGAAGAGAACTAAGAGTA+TGG + chr4.4:52431193-52431212 MS.gene070773:CDS 35.0%
AGAGCTTGAACAAACGAAAA+TGG + chr4.4:52432376-52432395 MS.gene070773:CDS 35.0%
AGGAATTTCCAAAACTCTGA+GGG - chr4.4:52434241-52434260 None:intergenic 35.0%
ATTGGCTACTGATAAGGTTA+AGG + chr4.4:52432595-52432614 MS.gene070773:CDS 35.0%
CAAGGTTGTTAGTATCATCA+GGG - chr4.4:52430688-52430707 None:intergenic 35.0%
CCAAGGAAATTTATGGAACT+TGG + chr4.4:52432572-52432591 MS.gene070773:CDS 35.0%
CCAAGTTCCATAAATTTCCT+TGG - chr4.4:52432575-52432594 None:intergenic 35.0%
CGTAAGGGTAAGAAAGTTAA+AGG + chr4.4:52432485-52432504 MS.gene070773:CDS 35.0%
GAGAAAAAGAGAACTGAGAA+TGG + chr4.4:52432536-52432555 MS.gene070773:CDS 35.0%
GATAGTCCTAAGTAAGAATG+AGG + chr4.4:52432955-52432974 MS.gene070773:CDS 35.0%
GATCTCACTATTACAATGCA+TGG + chr4.4:52432161-52432180 MS.gene070773:intron 35.0%
GCTGCTATTACATCCATTAT+TGG + chr4.4:52434431-52434450 MS.gene070773:CDS 35.0%
GGTATTGCCAATATTTGTGA+AGG - chr4.4:52434344-52434363 None:intergenic 35.0%
GTTCTCTTCTCTTCTGAATT+AGG - chr4.4:52431185-52431204 None:intergenic 35.0%
GTTGCAATACTTGAAGAACA+AGG - chr4.4:52434119-52434138 None:intergenic 35.0%
GTTTCATGAGATGATGAGAT+TGG - chr4.4:52430806-52430825 None:intergenic 35.0%
TATGGGCAAAAACTATCGAA+AGG + chr4.4:52433182-52433201 MS.gene070773:CDS 35.0%
TCAAGGTTGTTAGTATCATC+AGG - chr4.4:52430689-52430708 None:intergenic 35.0%
TCACGTAATAAGCACATTGA+TGG - chr4.4:52433781-52433800 None:intergenic 35.0%
TCATGAAACAAGAACAGAGT+TGG + chr4.4:52430817-52430836 MS.gene070773:CDS 35.0%
TCCCTTTCCTAGTCAATATA+AGG + chr4.4:52430756-52430775 MS.gene070773:CDS 35.0%
TCGACACTCTTTAGATTTGA+AGG + chr4.4:52431328-52431347 MS.gene070773:intron 35.0%
TTAACGTGGTAGCATATCAT+TGG - chr4.4:52431709-52431728 None:intergenic 35.0%
TTATTGCATTCAGCAAATCC+AGG - chr4.4:52433807-52433826 None:intergenic 35.0%
TTTCAGATGGCTATTCTTCA+AGG + chr4.4:52432353-52432372 MS.gene070773:intron 35.0%
! CCCTCAATACTCAAATTTTC+AGG + chr4.4:52431946-52431965 MS.gene070773:intron 35.0%
! CCCTGAAAATTTGAGTATTG+AGG - chr4.4:52431950-52431969 None:intergenic 35.0%
! GACCTTATATTGACTAGGAA+AGG - chr4.4:52430761-52430780 None:intergenic 35.0%
! GATGTCAATGGAGAAAGTAT+GGG + chr4.4:52433165-52433184 MS.gene070773:CDS 35.0%
! GGGAAAGAGTTGTTATTAAC+TGG - chr4.4:52430668-52430687 None:intergenic 35.0%
! TAGCTAAGTAGCTACCATTT+TGG - chr4.4:52433838-52433857 None:intergenic 35.0%
!! AAAGGAAAAGAAGTGTTCAC+TGG + chr4.4:52432743-52432762 MS.gene070773:CDS 35.0%
!! AGAGGTTTGATTTTCATTGG+AGG - chr4.4:52430783-52430802 None:intergenic 35.0%
!! ATGTTTTATTCGAACGTGAG+AGG + chr4.4:52432283-52432302 MS.gene070773:intron 35.0%
!! CGATAATAAGCTTTAGGACA+CGG - chr4.4:52433212-52433231 None:intergenic 35.0%
!! GAAATTTGATGATGCTGGAT+TGG + chr4.4:52432697-52432716 MS.gene070773:CDS 35.0%
!! GATAATAAGCTTTAGGACAC+GGG - chr4.4:52433211-52433230 None:intergenic 35.0%
!! GATCAATCTATGATGGATGA+TGG + chr4.4:52431152-52431171 MS.gene070773:CDS 35.0%
!! GTAGCATCCATGTTTTTGTT+GGG - chr4.4:52430725-52430744 None:intergenic 35.0%
!! TCTTTAGATTTGAAGGTGTC+TGG + chr4.4:52431335-52431354 MS.gene070773:intron 35.0%
!! TGGAGAGGTTTGATTTTCAT+TGG - chr4.4:52430786-52430805 None:intergenic 35.0%
!! TGTAGCATCCATGTTTTTGT+TGG - chr4.4:52430726-52430745 None:intergenic 35.0%
!!! TTCTTTTTTCGCATACAGCT+TGG + chr4.4:52431098-52431117 MS.gene070773:intron 35.0%
AAACTTACATGCTTTCGGAC+TGG - chr4.4:52433257-52433276 None:intergenic 40.0%
AACTTACATGCTTTCGGACT+GGG - chr4.4:52433256-52433275 None:intergenic 40.0%
ACATGTGGCATCAACAATGA+TGG + chr4.4:52434578-52434597 MS.gene070773:CDS 40.0%
AGCTAGATCAGAAGAAACCA+TGG + chr4.4:52433033-52433052 MS.gene070773:CDS 40.0%
AGCTGACCCTTCACAAATAT+TGG + chr4.4:52434334-52434353 MS.gene070773:CDS 40.0%
ATATGATCATTGTCAGGGAC+AGG - chr4.4:52430890-52430909 None:intergenic 40.0%
ATGAGATGATGAGATTGGAG+AGG - chr4.4:52430801-52430820 None:intergenic 40.0%
ATGGTCGTTGTATCGAAGAT+TGG - chr4.4:52433762-52433781 None:intergenic 40.0%
CATGTGGCATCAACAATGAT+GGG + chr4.4:52434579-52434598 MS.gene070773:CDS 40.0%
CATGTTTGTGACTGTGATCT+AGG - chr4.4:52433453-52433472 None:intergenic 40.0%
CCTAACTGATCTTGACTTCT+AGG - chr4.4:52432899-52432918 None:intergenic 40.0%
CCTAGAAGTCAAGATCAGTT+AGG + chr4.4:52432896-52432915 MS.gene070773:CDS 40.0%
CTGCAGCGATAATAAGCTTT+AGG - chr4.4:52433218-52433237 None:intergenic 40.0%
GACAAAGCTGCAGGAATATT+TGG - chr4.4:52434410-52434429 None:intergenic 40.0%
GACCCACGTACACAAAAATA+TGG + chr4.4:52431390-52431409 MS.gene070773:intron 40.0%
GAGGAATTTCCAAAACTCTG+AGG - chr4.4:52434242-52434261 None:intergenic 40.0%
GATTTGATATGCCTTGCACA+TGG + chr4.4:52433571-52433590 MS.gene070773:intron 40.0%
GCATCAACATCAAGCAATGA+TGG + chr4.4:52434056-52434075 MS.gene070773:CDS 40.0%
GGATCATATTGGTGCAATGA+CGG - chr4.4:52431974-52431993 None:intergenic 40.0%
GGATGTCAATGGAGAAAGTA+TGG + chr4.4:52433164-52433183 MS.gene070773:CDS 40.0%
GTACACAAAAATATGGGCCA+AGG + chr4.4:52431397-52431416 MS.gene070773:intron 40.0%
TATGATCATTGTCAGGGACA+GGG - chr4.4:52430889-52430908 None:intergenic 40.0%
TGGCTGAGTATCACCAATAA+TGG - chr4.4:52434447-52434466 None:intergenic 40.0%
TGGGACTAAGTTTCAACTCA+AGG - chr4.4:52430706-52430725 None:intergenic 40.0%
TTAAAAGAAAGCGAGGACCT+TGG - chr4.4:52431417-52431436 None:intergenic 40.0%
TTACGCACCTAACACATCAA+TGG + chr4.4:52433698-52433717 MS.gene070773:intron 40.0%
TTGTTATCCAACCATGTGCA+AGG - chr4.4:52433585-52433604 None:intergenic 40.0%
! AGATCACTGATGGATGTCAA+TGG + chr4.4:52433153-52433172 MS.gene070773:intron 40.0%
! CCAAAACTCTGAGGGATTAA+AGG - chr4.4:52434233-52434252 None:intergenic 40.0%
! CCTTTAATCCCTCAGAGTTT+TGG + chr4.4:52434230-52434249 MS.gene070773:CDS 40.0%
! GAAGAGAACTAAGAGTATGG+AGG + chr4.4:52431196-52431215 MS.gene070773:CDS 40.0%
! TCTAGGTTTTCTCATTGACG+AGG - chr4.4:52432882-52432901 None:intergenic 40.0%
! TGGAGGACCTTATATTGACT+AGG - chr4.4:52430766-52430785 None:intergenic 40.0%
!! AACTGATTTGCGTTCCTTTG+TGG - chr4.4:52434203-52434222 None:intergenic 40.0%
!! ACTTGGATTGGCTACTGATA+AGG + chr4.4:52432589-52432608 MS.gene070773:CDS 40.0%
!! GCCCATATTTTTGTGTACGT+GGG - chr4.4:52431395-52431414 None:intergenic 40.0%
!! GCTACCATTGCTATTTCCTT+CGG - chr4.4:52434519-52434538 None:intergenic 40.0%
AATGGCAACAATGTCACCGA+AGG + chr4.4:52434500-52434519 MS.gene070773:CDS 45.0%
ACCTGCAGCAGCTAAAGAAA+TGG + chr4.4:52433995-52434014 MS.gene070773:CDS 45.0%
ACTGGGGCCAAGGAAATTTA+TGG + chr4.4:52432565-52432584 MS.gene070773:CDS 45.0%
AGAGTGATGCAAGACCGTAA+GGG + chr4.4:52432470-52432489 MS.gene070773:CDS 45.0%
CAATGTGTGACACCCATACA+GGG + chr4.4:52431364-52431383 MS.gene070773:intron 45.0%
CTTTAGCTGCTGCAGGTAAA+GGG - chr4.4:52433992-52434011 None:intergenic 45.0%
GCAATGGCCATTGATGTGTT+AGG - chr4.4:52433708-52433727 None:intergenic 45.0%
GCAGAAATGCATGACTCTCA+TGG - chr4.4:52431596-52431615 None:intergenic 45.0%
GGACAGAGACAACTTCATGA+TGG - chr4.4:52430868-52430887 None:intergenic 45.0%
GTCACCGAAGGAAATAGCAA+TGG + chr4.4:52434512-52434531 MS.gene070773:CDS 45.0%
GTGCTTATTACGTGAATGCC+TGG + chr4.4:52433786-52433805 MS.gene070773:intron 45.0%
TCAATGTGTGACACCCATAC+AGG + chr4.4:52431363-52431382 MS.gene070773:intron 45.0%
TCTTTAGCTGCTGCAGGTAA+AGG - chr4.4:52433993-52434012 None:intergenic 45.0%
TGATATGCCTTGCACATGGT+TGG + chr4.4:52433575-52433594 MS.gene070773:intron 45.0%
TGATGGGAAGGAACGAAATC+AGG + chr4.4:52434595-52434614 MS.gene070773:CDS 45.0%
! AACTGAGAATGGTGGAGTAC+TGG + chr4.4:52432547-52432566 MS.gene070773:CDS 45.0%
! ACTGAGAATGGTGGAGTACT+GGG + chr4.4:52432548-52432567 MS.gene070773:CDS 45.0%
! ACTTCAGAATGGTGGAGAGT+TGG + chr4.4:52432787-52432806 MS.gene070773:CDS 45.0%
! GCGTTCCTTTGTGGAGTATT+TGG - chr4.4:52434194-52434213 None:intergenic 45.0%
! GTATGGGTGTCACACATTGA+CGG - chr4.4:52431363-52431382 None:intergenic 45.0%
! TATGGGTGTCACACATTGAC+GGG - chr4.4:52431362-52431381 None:intergenic 45.0%
! TGGCATCAACAATGATGGGA+AGG + chr4.4:52434583-52434602 MS.gene070773:CDS 45.0%
!! GGCCCATATTTTTGTGTACG+TGG - chr4.4:52431396-52431415 None:intergenic 45.0%
!! GTTGGTGTCAAGGCAATGTA+AGG + chr4.4:52432805-52432824 MS.gene070773:CDS 45.0%
!! TGCTGGATTGGCTACTGATA+AGG + chr4.4:52432709-52432728 MS.gene070773:CDS 45.0%
!!! TTATTTTCTAAAATTATTAT+TGG + chr4.4:52432021-52432040 MS.gene070773:intron 5.0%
!!! TTTTATATTTTTCTTTTATT+TGG + chr4.4:52431848-52431867 MS.gene070773:intron 5.0%
ATAGCAGCTGACAAAGCTGC+AGG - chr4.4:52434419-52434438 None:intergenic 50.0%
GAGAGTGATGCAAGACCGTA+AGG + chr4.4:52432469-52432488 MS.gene070773:CDS 50.0%
GCCATTTCTTTAGCTGCTGC+AGG - chr4.4:52433999-52434018 None:intergenic 50.0%
TTGAGGGATCAGAGAAGCTG+AGG - chr4.4:52434261-52434280 None:intergenic 50.0%
! GCAGCAGCAGTGTTGTTGTT+TGG - chr4.4:52434467-52434486 None:intergenic 50.0%
!! ATGGTGGAGAGTTGGTGTCA+AGG + chr4.4:52432795-52432814 MS.gene070773:CDS 50.0%
!! CTGAGAATGGTGGAGTACTG+GGG + chr4.4:52432549-52432568 MS.gene070773:CDS 50.0%
ACGTGGGTCTGTCCCTGTAT+GGG - chr4.4:52431379-52431398 None:intergenic 55.0%
TACGTGGGTCTGTCCCTGTA+TGG - chr4.4:52431380-52431399 None:intergenic 55.0%
TAGCTGCTGCAGGTAAAGGG+TGG - chr4.4:52433989-52434008 None:intergenic 55.0%
! CTTTCGGACTGGGCAACCAT+GGG - chr4.4:52433246-52433265 None:intergenic 55.0%
TATCGCTGCAGCACGACCCA+TGG + chr4.4:52433227-52433246 MS.gene070773:CDS 60.0%
! GCTTTCGGACTGGGCAACCA+TGG - chr4.4:52433247-52433266 None:intergenic 60.0%
!! ATGGTGGAGTACTGGGGCCA+AGG + chr4.4:52432555-52432574 MS.gene070773:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 52430653 52434667 52430653 ID=MS.gene070773
chr4.4 mRNA 52430653 52434667 52430653 ID=MS.gene070773.t1;Parent=MS.gene070773
chr4.4 exon 52430653 52430949 52430653 ID=MS.gene070773.t1.exon1;Parent=MS.gene070773.t1
chr4.4 CDS 52430653 52430949 52430653 ID=cds.MS.gene070773.t1;Parent=MS.gene070773.t1
chr4.4 exon 52431116 52431217 52431116 ID=MS.gene070773.t1.exon2;Parent=MS.gene070773.t1
chr4.4 CDS 52431116 52431217 52431116 ID=cds.MS.gene070773.t1;Parent=MS.gene070773.t1
chr4.4 exon 52432359 52433054 52432359 ID=MS.gene070773.t1.exon3;Parent=MS.gene070773.t1
chr4.4 CDS 52432359 52433054 52432359 ID=cds.MS.gene070773.t1;Parent=MS.gene070773.t1
chr4.4 exon 52433155 52433268 52433155 ID=MS.gene070773.t1.exon4;Parent=MS.gene070773.t1
chr4.4 CDS 52433155 52433268 52433155 ID=cds.MS.gene070773.t1;Parent=MS.gene070773.t1
chr4.4 exon 52433927 52434667 52433927 ID=MS.gene070773.t1.exon5;Parent=MS.gene070773.t1
chr4.4 CDS 52433927 52434667 52433927 ID=cds.MS.gene070773.t1;Parent=MS.gene070773.t1
Gene Sequence

>MS.gene070773

ATGGCTTCAGTTCCAGTTAATAACAACTCTTTCCCTGATGATACTAACAACCTTGAGTTGAAACTTAGTCCCAACAAAAACATGGATGCTACACATAGTACTATCCCTTTCCTAGTCAATATAAGGTCCTCCAATGAAAATCAAACCTCTCCAATCTCATCATCTCATGAAACAAGAACAGAGTTGGATTTCTTTGATGACAACAACAATGACCATCATGAAGTTGTCTCTGTCCCTGTCCCTGACAATGATCATATTCATACTGTTACACCATCTTTATTGGAACTAAAGCTAAGCGTGAGTGACCATAATTCAATATCCCATTGTTATTATTTTTTTTACGTATTATATTATATATATTGTTTTTAAATTTCATAGCTTTTGAATCTGTAATCGCAAAAAAAAACATTTATTTTTTATGATTATCTTTTTAATTTGATGAATATTCTTTTTTCGCATACAGCTTGGTCCCATTCCTATTACTATCAATACTAGTAGTGATCAATCTATGATGGATGATGGCATGTCACCTAATTCAGAAGAGAAGAGAACTAAGAGTATGGAGGTAATGTTATTTTTATTAAGACCTAGCAGAATTTAATGAAATTATATATGATGAACATTTTTTTTCCTTTAATTTTATTGAGTCAAAACTGGGTGATTTTATATATTGCATCGACACTCTTTAGATTTGAAGGTGTCTGGACCCGTCAATGTGTGACACCCATACAGGGACAGACCCACGTACACAAAAATATGGGCCAAGGTCCTCGCTTTCTTTTAAATTTTTTCTACTATGTTATTCTTTTTGGAAAGGAAATATATATATATATATATATATATATATATGATTCTTGGTTGGTTTGGCTTGTTAGTTTTTGTCTCTTACTCTTATATGACCTACATTTATAAGTATAATACAATATAATGTTATATGCAACCATGAGAGTCATGCATTTCTGCTCTTTATTTCAACCACAAATAATAATTATGTTATATTGAGTTAATTTAACTAGTATATTTTGTCAACGTACAATTATTTTATACATGCATCCAATGATATGCTACCACGTTAATTAATGAATATGACAACTCATGTGTTTTCATATTTATTAATTGATGTAGCAGCACATCATTGAATGAATGTGTAAAATAATTGTACACCGACATAGTACGCTAATTAAATTCTTTCATATTTTATATTTTTCTTTTATTTGGTTTCATTTTTTAAAAGATACTAGTTTAATATTTTAAATGTAAATTGAATTTATTTACTCATTATTTTAAATGCTGCCCTCAATACTCAAATTTTCAGGGTCCGTCATTGCACCAATATGATCCTGACATAAACTGTTACATTTTGTTGTTTTATTTTCTAAAATTATTATTGGTTGTTGATGTGTCAGTTTTAATGTTTTGTCCGATTTTATATGTATGTCAGTGTTTATAGAATATTTTTGTGATTTGAATCCTCTTCTTTGTGAAAAAAATTACTGAGTTATGTTAATGATCTCACTATTACAATGCATGGCCTTTTAGAACACTGTTAACATGATGGGGTCCAATGTATTTTGATTTTGTTGGATAATCGAATGCAATTTAATAGTGAAATGATTATTATAACCCTAACATGTTTTATTCGAACGTGAGAGGATCCAAATCCGTTTTTAATGTGATTATATTTGAGTTCATTGAACTATTTTCAGATGGCTATTCTTCAAGGAGAGCTTGAACAAACGAAAATGGAGAACTGTAGACTAAAATTGATGTATGATCTGCTTAGAATAGATTACAATTACATGCGGAAGCGTTTCGAGAGAGTGATGCAAGACCGTAAGGGTAAGAAAGTTAAAGGAAAAAAAGTCTTTGATGGAAAATTTAAAGAGAAAAAGAGAACTGAGAATGGTGGAGTACTGGGGCCAAGGAAATTTATGGAACTTGGATTGGCTACTGATAAGGTTAAGGAAGTTAAAGGACAAAAAGTTTCTGATCAAAAATTTGTAGAGAAAATGAGAATTAAGAATGATGGAGAATTAATGAAAAGGAAATTTGATGATGCTGGATTGGCTACTGATAAGGTTAAGGAAGTTAAAGGAAAAGAAGTGTTCACTGGAAAATGTGAAAAGAAAAAGAGACTTCAGAATGGTGGAGAGTTGGTGTCAAGGCAATGTAAGGATCTCGTGTTGATTACAAATGCTGATATTGTGATGGATCACGAAGCTTCGTCCTCGTCAATGAGAAAACCTAGAAGTCAAGATCAGTTAGGATCGGCGATGAAAAACATCGATGTTGCTTCGAATGAGATAGTCCTAAGTAAGAATGAGGATGTTAATTTTGATAATGTTGAGGATTCATTGACAAAAGCAAGAGTTTGTATTAGAGCTAGATCAGAAGAAACCATGGTAAGTATGTAAAAGTGTATATTAACTTTCAACTTTGCAAGTTTTTATATCATAATTTATTGTCCTTTTTACTAAAAAGAATTAATTTTTATTGAGTTAGATCACTGATGGATGTCAATGGAGAAAGTATGGGCAAAAACTATCGAAAGGAAACCCGTGTCCTAAAGCTTATTATCGCTGCAGCACGACCCATGGTTGCCCAGTCCGAAAGCATGTAAGTTTCTTCTGTAAATATCCAAATTACATGTATTTACTAAAGTTTTAAATTTATGTAGCCCTATTGATTGCTTTCGCATTATTATATTTAATAGTATTATAGAGAATTATAGTCAAATATGATTAATGCAACGTCAATAACATTAAATTCACAACGACATTAAACTCTTTGATTGTTGCCTAGATCACAGTCACAAACATGCATATTTTAAAACATTGGTCTTTAAATCATTATTTTATGTATGTTTTCAATTAATTAAAAATTAACTCCACATACAGAAACATTTTTAGTAATTGATTGATTTGATATGCCTTGCACATGGTTGGATAACAATATAATTTAATTGAAATTTGTTTCCACTTTCAAAGCTTATGTTTCTATGCTAGAAAATGGATTTGTATTTGCTCCATTCAGCAATCTATGTATTTACGCACCTAACACATCAATGGCCATTGCAAATCAAAATATGCAGTTATGTTTGGATCATCCAATCTTCGATACAACGACCATCAATGTGCTTATTACGTGAATGCCTGGATTTGCTGAATGCAATAAACTCAAATCCAAAATGGTAGCTACTTAGCTAGGTTAATTAAATTACTTATGAAGTGGTCTGTAATGTAATGACATATTTAATACAATTAAAACTTAACAGGTACAAAGGTGTGCTTTTGACAAAACAGTTGTAATCACAACTTATGAAGGAAATCACAACCACCCTTTACCTGCAGCAGCTAAAGAAATGGCAAAAACAACTTCAGCAGCTGCAAAGATACTTCTTTCAGCATCAACATCAAGCAATGATGGACAATTAAATGCAAATTTACTCACAAGAACACTTCTTCCTTGTTCTTCAAGTATTGCAACTATATCAGCTTCTGCTCCATATCCAACTATTACAATTGATTACACTCAATCTCCAAATACTCCACAAAGGAACGCAAATCAGTTTCAAACTCCTTTAATCCCTCAGAGTTTTGGAAATTCCTCAGCTTCTCTGATCCCTCAAATACCTAATCAAAACCAATCAAAATTTTCTGGACTACATATGTCGAAAGATGCAGCTGACCCTTCACAAATATTGGCAATACCAAATATTACTGCTCAAACAGTTAATGTTGCCATTGTTGCTAGTCCAAATATTCCTGCAGCTTTGTCAGCTGCTATTACATCCATTATTGGTGATACTCAGCCAAACAACAACACTGCTGCTGCTGATAATAATAATAATGGCAACAATGTCACCGAAGGAAATAGCAATGGTAGCAACAATAATGTCACAGAAGCCAATAGCAACAAAATTATCACATGTGGCATCAACAATGATGGGAAGGAACGAAATCAGGAATACCAATTACAAGACCTAAACTTTAATTTTTTTTGCTCAAAGATTTAG
Protein sequence

>MS.gene070773.t1

MASVPVNNNSFPDDTNNLELKLSPNKNMDATHSTIPFLVNIRSSNENQTSPISSSHETRTELDFFDDNNNDHHEVVSVPVPDNDHIHTVTPSLLELKLSLGPIPITINTSSDQSMMDDGMSPNSEEKRTKSMEMAILQGELEQTKMENCRLKLMYDLLRIDYNYMRKRFERVMQDRKGKKVKGKKVFDGKFKEKKRTENGGVLGPRKFMELGLATDKVKEVKGQKVSDQKFVEKMRIKNDGELMKRKFDDAGLATDKVKEVKGKEVFTGKCEKKKRLQNGGELVSRQCKDLVLITNADIVMDHEASSSSMRKPRSQDQLGSAMKNIDVASNEIVLSKNEDVNFDNVEDSLTKARVCIRARSEETMITDGCQWRKYGQKLSKGNPCPKAYYRCSTTHGCPVRKHVQRCAFDKTVVITTYEGNHNHPLPAAAKEMAKTTSAAAKILLSASTSSNDGQLNANLLTRTLLPCSSSIATISASAPYPTITIDYTQSPNTPQRNANQFQTPLIPQSFGNSSASLIPQIPNQNQSKFSGLHMSKDAADPSQILAIPNITAQTVNVAIVASPNIPAALSAAITSIIGDTQPNNNTAAADNNNNGNNVTEGNSNGSNNNVTEANSNKIITCGINNDGKERNQEYQLQDLNFNFFCSKI