AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071014

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071014.t1 MTR_7g069740 91.327 588 47 2 1 587 1 585 0.0 1100
MS.gene071014.t1 MTR_3g072710 27.653 311 197 11 285 585 207 499 8.49e-18 87.0
MS.gene071014.t1 MTR_5g058860 26.368 402 244 15 217 585 114 496 3.00e-17 85.5
MS.gene071014.t1 MTR_7g027190 25.799 438 233 21 201 586 276 673 1.01e-14 77.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071014.t1 AT1G07520 23.645 406 247 16 217 585 313 692 2.60e-12 70.1
MS.gene071014.t1 AT1G07520 23.645 406 247 16 217 585 314 693 2.84e-12 70.1
MS.gene071014.t1 AT1G07520 23.645 406 247 16 217 585 149 528 1.31e-11 67.8
MS.gene071014.t1 AT1G07520 23.645 406 247 16 217 585 149 528 1.31e-11 67.8

Find 95 sgRNAs with CRISPR-Local

Find 130 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAATTGACATCAATTAAA+TGG 0.189338 7.3:-35174824 MS.gene071014:CDS
CACATAATTGATTATTGTTT+TGG 0.189427 7.3:-35174903 MS.gene071014:CDS
ACCAATTTGTGGACTCAAAA+TGG 0.208342 7.3:+35175496 None:intergenic
TTTCTTCATCAATTGGAAAT+TGG 0.210829 7.3:+35175561 None:intergenic
TACTTTGCCACAAGAAAATA+TGG 0.244532 7.3:-35175252 MS.gene071014:CDS
AAATGAGAATCAACTTGTTT+TGG 0.251878 7.3:-35174169 MS.gene071014:CDS
AACTCCCTTCACTTTCTTCT+TGG 0.260798 7.3:+35175453 None:intergenic
CAATTTCAAACCACATGATT+TGG 0.267276 7.3:+35174737 None:intergenic
CTTGTATATCTTCTCTTGAT+TGG 0.318337 7.3:-35174341 MS.gene071014:CDS
GATATGATCATTGGTGAATA+TGG 0.332452 7.3:+35175583 None:intergenic
TCCAAGGTTTCTTCATCAAT+TGG 0.335882 7.3:+35175554 None:intergenic
GATGACCGATCTTCCTCATA+TGG 0.340403 7.3:-35174622 MS.gene071014:CDS
TCTATGTGTTCATAGATTAA+AGG 0.348224 7.3:+35175830 None:intergenic
ATACTCATAAAGTTCCCTTT+TGG 0.349243 7.3:+35174761 None:intergenic
CTTGGAACTTCCATCTCTAA+TGG 0.352756 7.3:-35175537 MS.gene071014:CDS
ATGAAAATTCTGCATCTGTT+TGG 0.356641 7.3:-35174797 MS.gene071014:CDS
AAAGATGAGATTAGCATAGA+AGG 0.374138 7.3:-35174279 MS.gene071014:CDS
GTTGCATTATAAAGCTTTGT+TGG 0.380439 7.3:-35174436 MS.gene071014:CDS
TCTTGTATGCCTCGCCTTTG+AGG 0.385451 7.3:+35175042 None:intergenic
CCTCGGAGACAGTTGTAAAA+TGG 0.387068 7.3:-35175381 MS.gene071014:CDS
AGGAAGGGAATTGTTACTTT+TGG 0.387523 7.3:-35174525 MS.gene071014:CDS
AGTGAGTCTTCCACACTTGA+TGG 0.390562 7.3:-35175216 MS.gene071014:CDS
GAAGGTAGAGGAAAAGGAAT+TGG 0.405087 7.3:-35174715 MS.gene071014:CDS
GAAATTGAAGGTAGAGGAAA+AGG 0.406350 7.3:-35174721 MS.gene071014:CDS
TCTTGAATATTACCAATTTG+TGG 0.407532 7.3:+35175485 None:intergenic
CTCTTGATTGGTTACAAACA+TGG 0.411194 7.3:-35174329 MS.gene071014:CDS
TGAGACACTTGCATATTACT+TGG 0.425483 7.3:-35175093 MS.gene071014:CDS
TTTATCAAGGGAATTTGGTT+GGG 0.433000 7.3:-35174995 MS.gene071014:CDS
TCAACCATGGTGAAGCTATA+CGG 0.433516 7.3:+35175764 None:intergenic
ATTTGATCAAGAACTATTGT+AGG 0.434003 7.3:-35174545 MS.gene071014:CDS
TCCAATTGATGAAGAAACCT+TGG 0.445970 7.3:-35175555 MS.gene071014:CDS
GAAACAGTCTCCAATAGAAA+AGG 0.449571 7.3:+35174864 None:intergenic
TGGAAATTGGATATGATCAT+TGG 0.449728 7.3:+35175574 None:intergenic
GATCAAAATAATGAACGAAA+AGG 0.458900 7.3:-35174676 MS.gene071014:CDS
TCATATCAAGCAAGAATTGA+AGG 0.470729 7.3:-35174195 MS.gene071014:CDS
TGCATCTGTTTGGAATTTCG+AGG 0.475745 7.3:-35174787 MS.gene071014:CDS
ATGACCGATCTTCCTCATAT+GGG 0.477572 7.3:-35174621 MS.gene071014:CDS
CTTCTAACCATTCCCATATG+AGG 0.492028 7.3:+35174609 None:intergenic
ATCAAAATAATGAACGAAAA+GGG 0.498103 7.3:-35174675 MS.gene071014:CDS
TCTGATTTCATGGAAAGTGT+TGG 0.499305 7.3:+35175299 None:intergenic
GGAATTTCGAGGATACCAAA+AGG 0.507394 7.3:-35174776 MS.gene071014:CDS
TCATCCATGATGATGCCATA+TGG 0.508726 7.3:+35175722 None:intergenic
TCATCCATGATGATGCCATA+TGG 0.509020 7.3:+35175677 None:intergenic
CATTAGAGATGGAAGTTCCA+AGG 0.509956 7.3:+35175538 None:intergenic
ATCATGTGGTTTGAAATTGA+AGG 0.512511 7.3:-35174733 MS.gene071014:CDS
ATCAAGAACTATTGTAGGAA+GGG 0.515195 7.3:-35174540 MS.gene071014:CDS
GCTCAAGTAGTGACAAATCA+TGG 0.515932 7.3:-35175071 MS.gene071014:CDS
CATGGTGAAGCCTTGGAGAA+AGG 0.516575 7.3:-35175188 MS.gene071014:CDS
AAAATTCTCCCTGTGGTGCA+TGG 0.518660 7.3:-35175325 MS.gene071014:CDS
GAGAGTACTATTCTCTGAAG+TGG 0.519133 7.3:+35175616 None:intergenic
GTCTCAAGAGAGTCACCAAT+AGG 0.521912 7.3:+35175110 None:intergenic
GATCAAGAACTATTGTAGGA+AGG 0.523430 7.3:-35174541 MS.gene071014:CDS
GGAAGCACATGGTGAAGCCT+TGG 0.523810 7.3:-35175195 MS.gene071014:CDS
TGGTTTGAAATTGAAGGTAG+AGG 0.525487 7.3:-35174727 MS.gene071014:CDS
GATGATGACTATGTTCCATA+TGG 0.526351 7.3:-35175692 MS.gene071014:CDS
GATTCTAATTCCATTAGAGA+TGG 0.530035 7.3:+35175527 None:intergenic
GTTGATGACTATGATCCATA+TGG 0.530988 7.3:-35175737 MS.gene071014:CDS
GAATTTCGAGGATACCAAAA+GGG 0.531446 7.3:-35174775 MS.gene071014:CDS
AACAGATTCTTCACTCTCCA+TGG 0.536819 7.3:-35175853 MS.gene071014:CDS
GATGAGATTAGCATAGAAGG+TGG 0.537000 7.3:-35174276 MS.gene071014:CDS
TGTTGGACTCCATGCACCAC+AGG 0.537815 7.3:+35175316 None:intergenic
CGATCTTCCTCATATGGGAA+TGG 0.548107 7.3:-35174616 MS.gene071014:CDS
CCTTCACAAAATTCTCCCTG+TGG 0.548533 7.3:-35175332 MS.gene071014:CDS
GATTTGAACTATACCCTTCG+AGG 0.553155 7.3:+35175405 None:intergenic
TGTTCATAGATTAAAGGCCA+TGG 0.555928 7.3:+35175836 None:intergenic
GAAATGATCATAAGTGGCAC+TGG 0.557550 7.3:+35175355 None:intergenic
TGAAGGGAAATGATCATAAG+TGG 0.559314 7.3:+35175349 None:intergenic
TGATCCATATGGCATCATCA+TGG 0.565829 7.3:-35175726 MS.gene071014:CDS
TGTTCCATATGGCATCATCA+TGG 0.566239 7.3:-35175681 MS.gene071014:CDS
CATTCCCATATGAGGAAGAT+CGG 0.567563 7.3:+35174617 None:intergenic
CTATACCCTTCGAGGTCATG+TGG 0.569181 7.3:+35175413 None:intergenic
TCTTGATTGGTTACAAACAT+GGG 0.569487 7.3:-35174328 MS.gene071014:CDS
ATTGACATCAATTAAATGGA+GGG 0.571443 7.3:-35174820 MS.gene071014:CDS
AATTGACATCAATTAAATGG+AGG 0.573165 7.3:-35174821 MS.gene071014:CDS
TGAGTGTGAAGTGTTGTTTG+TGG 0.576764 7.3:-35174367 MS.gene071014:CDS
TCAAGTGTGGAAGACTCACT+TGG 0.579458 7.3:+35175219 None:intergenic
TATGAGTATGCCAAATCATG+TGG 0.582299 7.3:-35174747 MS.gene071014:CDS
CAATCAAGAGAAGATATACA+AGG 0.583066 7.3:+35174342 None:intergenic
GAAGTCAGAGAAATGTTGAG+AGG 0.583320 7.3:-35174228 MS.gene071014:CDS
TGGGAAGAGATGAAAAGTGA+TGG 0.606712 7.3:-35174309 MS.gene071014:CDS
GAATACCAAGAAGAAAGTGA+AGG 0.607663 7.3:-35175458 MS.gene071014:CDS
AATACCAAGAAGAAAGTGAA+GGG 0.608570 7.3:-35175457 MS.gene071014:CDS
AATCATGGAGATTACCTCAA+AGG 0.616214 7.3:-35175056 MS.gene071014:CDS
CCATGTGCTTCCATCAAGTG+TGG 0.618160 7.3:+35175206 None:intergenic
ATGATCATAAGTGGCACTGG+CGG 0.631598 7.3:+35175358 None:intergenic
GTCATCAACATGGTCAACCA+TGG 0.639550 7.3:+35175751 None:intergenic
GGGTATAGTTCAAATCCCCT+CGG 0.648026 7.3:-35175398 MS.gene071014:CDS
CCACACTTGATGGAAGCACA+TGG 0.654957 7.3:-35175206 MS.gene071014:CDS
AAGAGAGTCACCAATAGGAG+TGG 0.663623 7.3:+35175115 None:intergenic
GTTGGACTCCATGCACCACA+GGG 0.676745 7.3:+35175317 None:intergenic
AAAATAATGAACGAAAAGGG+CGG 0.679821 7.3:-35174672 MS.gene071014:CDS
TGGAGATTACCTCAAAGGCG+AGG 0.686092 7.3:-35175051 MS.gene071014:CDS
GAACATAGTCATCATCAACA+TGG 0.707488 7.3:+35175699 None:intergenic
ATGGATCATAGTCATCAACA+TGG 0.710348 7.3:+35175741 None:intergenic
TGAGCCGTATAGCTTCACCA+TGG 0.712624 7.3:-35175768 MS.gene071014:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAATAGTTTGAATTTCA+AGG - chr7.3:35175507-35175526 MS.gene071014:CDS 15.0%
!! ATCAAAATAATGAACGAAAA+GGG - chr7.3:35175310-35175329 MS.gene071014:CDS 20.0%
!! TGAAATTGACATCAATTAAA+TGG - chr7.3:35175161-35175180 MS.gene071014:CDS 20.0%
!!! AACTGTTAAGATTTTCTATT+TGG - chr7.3:35175854-35175873 MS.gene071014:CDS 20.0%
!!! ATTGATTATTGTTTTGGAAA+TGG - chr7.3:35175088-35175107 MS.gene071014:CDS 20.0%
!!! CACATAATTGATTATTGTTT+TGG - chr7.3:35175082-35175101 MS.gene071014:CDS 20.0%
! AATTGACATCAATTAAATGG+AGG - chr7.3:35175164-35175183 MS.gene071014:CDS 25.0%
! ATTGACATCAATTAAATGGA+GGG - chr7.3:35175165-35175184 MS.gene071014:CDS 25.0%
! ATTTGATCAAGAACTATTGT+AGG - chr7.3:35175440-35175459 MS.gene071014:CDS 25.0%
! GATCAAAATAATGAACGAAA+AGG - chr7.3:35175309-35175328 MS.gene071014:CDS 25.0%
! TCTATGTGTTCATAGATTAA+AGG + chr7.3:35174158-35174177 None:intergenic 25.0%
! TCTTGAATATTACCAATTTG+TGG + chr7.3:35174503-35174522 None:intergenic 25.0%
! TGATGAAAAATCTGATTTCA+TGG + chr7.3:35174699-35174718 None:intergenic 25.0%
! TTTCTTCATCAATTGGAAAT+TGG + chr7.3:35174427-35174446 None:intergenic 25.0%
!! TCAATTTCCATATTTTCTTG+TGG + chr7.3:35174743-35174762 None:intergenic 25.0%
!!! AAATGAGAATCAACTTGTTT+TGG - chr7.3:35175816-35175835 MS.gene071014:CDS 25.0%
!!! AATTTCAAGGCATTTTTTGA+AGG - chr7.3:35175520-35175539 MS.gene071014:CDS 25.0%
!!! ATTGAGCAAAAATGTTTTGA+TGG - chr7.3:35175735-35175754 MS.gene071014:CDS 25.0%
!!! ATTTCAAGGCATTTTTTGAA+GGG - chr7.3:35175521-35175540 MS.gene071014:CDS 25.0%
!!! CTTTTCAGGTATTTTATCAA+GGG - chr7.3:35174978-35174997 MS.gene071014:CDS 25.0%
AAAATAATGAACGAAAAGGG+CGG - chr7.3:35175313-35175332 MS.gene071014:CDS 30.0%
AAAGATGAGATTAGCATAGA+AGG - chr7.3:35175706-35175725 MS.gene071014:CDS 30.0%
AATACCAAGAAGAAAGTGAA+GGG - chr7.3:35174528-35174547 MS.gene071014:CDS 30.0%
ATCAAGAACTATTGTAGGAA+GGG - chr7.3:35175445-35175464 MS.gene071014:CDS 30.0%
ATGAAAATTCTGCATCTGTT+TGG - chr7.3:35175188-35175207 MS.gene071014:CDS 30.0%
CAATCAAGAGAAGATATACA+AGG + chr7.3:35175646-35175665 None:intergenic 30.0%
CAATTTCAAACCACATGATT+TGG + chr7.3:35175251-35175270 None:intergenic 30.0%
CTTGTATATCTTCTCTTGAT+TGG - chr7.3:35175644-35175663 MS.gene071014:CDS 30.0%
GATATGATCATTGGTGAATA+TGG + chr7.3:35174405-35174424 None:intergenic 30.0%
GATTCTAATTCCATTAGAGA+TGG + chr7.3:35174461-35174480 None:intergenic 30.0%
TACTTTGCCACAAGAAAATA+TGG - chr7.3:35174733-35174752 MS.gene071014:CDS 30.0%
TCATATCAAGCAAGAATTGA+AGG - chr7.3:35175790-35175809 MS.gene071014:CDS 30.0%
TGGAAATTGGATATGATCAT+TGG + chr7.3:35174414-35174433 None:intergenic 30.0%
TTTATCAAGGGAATTTGGTT+GGG - chr7.3:35174990-35175009 MS.gene071014:CDS 30.0%
! ACATGCTTATGAGTTTTTGA+AGG - chr7.3:35175408-35175427 MS.gene071014:CDS 30.0%
! ATACTCATAAAGTTCCCTTT+TGG + chr7.3:35175227-35175246 None:intergenic 30.0%
! ATCATGTGGTTTGAAATTGA+AGG - chr7.3:35175252-35175271 MS.gene071014:CDS 30.0%
! ATGATGATGATGATGATCAA+AGG + chr7.3:35174189-35174208 None:intergenic 30.0%
! CATGTGGAAAATTTTGTGAA+GGG + chr7.3:35174559-35174578 None:intergenic 30.0%
! GGTATTTTATCAAGGGAATT+TGG - chr7.3:35174985-35175004 MS.gene071014:CDS 30.0%
! GTTGCATTATAAAGCTTTGT+TGG - chr7.3:35175549-35175568 MS.gene071014:CDS 30.0%
! TCATGTGGAAAATTTTGTGA+AGG + chr7.3:35174560-35174579 None:intergenic 30.0%
! TCTTGATTGGTTACAAACAT+GGG - chr7.3:35175657-35175676 MS.gene071014:CDS 30.0%
! TTTTATCAAGGGAATTTGGT+TGG - chr7.3:35174989-35175008 MS.gene071014:CDS 30.0%
!!! AGAATCAACTTGTTTTGGAA+TGG - chr7.3:35175821-35175840 MS.gene071014:CDS 30.0%
!!! GCTTTTCAGGTATTTTATCA+AGG - chr7.3:35174977-35174996 MS.gene071014:CDS 30.0%
AATCATGGAGATTACCTCAA+AGG - chr7.3:35174929-35174948 MS.gene071014:CDS 35.0%
ACCAATTTGTGGACTCAAAA+TGG + chr7.3:35174492-35174511 None:intergenic 35.0%
ATGGATCATAGTCATCAACA+TGG + chr7.3:35174247-35174266 None:intergenic 35.0%
GAAACAGTCTCCAATAGAAA+AGG + chr7.3:35175124-35175143 None:intergenic 35.0%
GAAATTGAAGGTAGAGGAAA+AGG - chr7.3:35175264-35175283 MS.gene071014:CDS 35.0%
GAACATAGTCATCATCAACA+TGG + chr7.3:35174289-35174308 None:intergenic 35.0%
GAATACCAAGAAGAAAGTGA+AGG - chr7.3:35174527-35174546 MS.gene071014:CDS 35.0%
GAATTTCGAGGATACCAAAA+GGG - chr7.3:35175210-35175229 MS.gene071014:CDS 35.0%
GATCAAGAACTATTGTAGGA+AGG - chr7.3:35175444-35175463 MS.gene071014:CDS 35.0%
GATGATGACTATGTTCCATA+TGG - chr7.3:35174293-35174312 MS.gene071014:CDS 35.0%
TATGAGTATGCCAAATCATG+TGG - chr7.3:35175238-35175257 MS.gene071014:CDS 35.0%
TCCAAGGTTTCTTCATCAAT+TGG + chr7.3:35174434-35174453 None:intergenic 35.0%
TCCAATTGATGAAGAAACCT+TGG - chr7.3:35174430-35174449 MS.gene071014:CDS 35.0%
TGAAGGGAAATGATCATAAG+TGG + chr7.3:35174639-35174658 None:intergenic 35.0%
TGAGACACTTGCATATTACT+TGG - chr7.3:35174892-35174911 MS.gene071014:CDS 35.0%
TGTTCATAGATTAAAGGCCA+TGG + chr7.3:35174152-35174171 None:intergenic 35.0%
! CTCTTGATTGGTTACAAACA+TGG - chr7.3:35175656-35175675 MS.gene071014:CDS 35.0%
! TCTGATTTCATGGAAAGTGT+TGG + chr7.3:35174689-35174708 None:intergenic 35.0%
! TGGTTTGAAATTGAAGGTAG+AGG - chr7.3:35175258-35175277 MS.gene071014:CDS 35.0%
! TGTTCAATGGCCTTTTCTAT+TGG - chr7.3:35175111-35175130 MS.gene071014:CDS 35.0%
!! GTTGATGACTATGATCCATA+TGG - chr7.3:35174248-35174267 MS.gene071014:CDS 35.0%
!! TCCATTTTGAGTCCACAAAT+TGG - chr7.3:35174488-35174507 MS.gene071014:CDS 35.0%
!!! AGGAAGGGAATTGTTACTTT+TGG - chr7.3:35175460-35175479 MS.gene071014:CDS 35.0%
!!! CAACTTGTTTTGGAATGGAA+AGG - chr7.3:35175826-35175845 MS.gene071014:CDS 35.0%
!!! GGAATTGTTACTTTTGGTGA+TGG - chr7.3:35175466-35175485 MS.gene071014:CDS 35.0%
!!! GTTTTGGAAATGGTGTTCAA+TGG - chr7.3:35175098-35175117 MS.gene071014:CDS 35.0%
AACAGATTCTTCACTCTCCA+TGG - chr7.3:35174132-35174151 MS.gene071014:CDS 40.0%
AACTCCCTTCACTTTCTTCT+TGG + chr7.3:35174535-35174554 None:intergenic 40.0%
ATGACCGATCTTCCTCATAT+GGG - chr7.3:35175364-35175383 MS.gene071014:CDS 40.0%
CAAAAAGTCACCACTCCTAT+TGG - chr7.3:35174860-35174879 MS.gene071014:CDS 40.0%
CATTAGAGATGGAAGTTCCA+AGG + chr7.3:35174450-35174469 None:intergenic 40.0%
CATTCCCATATGAGGAAGAT+CGG + chr7.3:35175371-35175390 None:intergenic 40.0%
CTTCTAACCATTCCCATATG+AGG + chr7.3:35175379-35175398 None:intergenic 40.0%
CTTGGAACTTCCATCTCTAA+TGG - chr7.3:35174448-35174467 MS.gene071014:CDS 40.0%
GAAATGATCATAAGTGGCAC+TGG + chr7.3:35174633-35174652 None:intergenic 40.0%
GAAGGTAGAGGAAAAGGAAT+TGG - chr7.3:35175270-35175289 MS.gene071014:CDS 40.0%
GAAGTCAGAGAAATGTTGAG+AGG - chr7.3:35175757-35175776 MS.gene071014:CDS 40.0%
GATGAGATTAGCATAGAAGG+TGG - chr7.3:35175709-35175728 MS.gene071014:CDS 40.0%
GATTTGAACTATACCCTTCG+AGG + chr7.3:35174583-35174602 None:intergenic 40.0%
GCTCAAGTAGTGACAAATCA+TGG - chr7.3:35174914-35174933 MS.gene071014:CDS 40.0%
GGAATTTCGAGGATACCAAA+AGG - chr7.3:35175209-35175228 MS.gene071014:CDS 40.0%
TCAACCATGGTGAAGCTATA+CGG + chr7.3:35174224-35174243 None:intergenic 40.0%
TGATCCATATGGCATCATCA+TGG - chr7.3:35174259-35174278 MS.gene071014:CDS 40.0%
TGGGAAGAGATGAAAAGTGA+TGG - chr7.3:35175676-35175695 MS.gene071014:CDS 40.0%
TGTTCCATATGGCATCATCA+TGG - chr7.3:35174304-35174323 MS.gene071014:CDS 40.0%
! AATTTTCCACATGACCTCGA+AGG - chr7.3:35174566-35174585 MS.gene071014:CDS 40.0%
! ATTTTACAACTGTCTCCGAG+GGG + chr7.3:35174605-35174624 None:intergenic 40.0%
! ATTTTCCACATGACCTCGAA+GGG - chr7.3:35174567-35174586 MS.gene071014:CDS 40.0%
! CAATAGGAGTGGTGACTTTT+TGG + chr7.3:35174862-35174881 None:intergenic 40.0%
! CACAGGGAGAATTTTGTGAA+GGG + chr7.3:35174655-35174674 None:intergenic 40.0%
! CATTTTACAACTGTCTCCGA+GGG + chr7.3:35174606-35174625 None:intergenic 40.0%
! CGAGGCATACAAGATTTTTC+AGG - chr7.3:35174952-35174971 MS.gene071014:CDS 40.0%
! TCATCCATGATGATGCCATA+TGG + chr7.3:35174266-35174285 None:intergenic 40.0%
! TGCATCTGTTTGGAATTTCG+AGG - chr7.3:35175198-35175217 MS.gene071014:CDS 40.0%
!! GAGAGTACTATTCTCTGAAG+TGG + chr7.3:35174372-35174391 None:intergenic 40.0%
!! TGAGTGTGAAGTGTTGTTTG+TGG - chr7.3:35175618-35175637 MS.gene071014:CDS 40.0%
!! TGAGTTTTTGAAGGTAGCTG+AGG - chr7.3:35175417-35175436 MS.gene071014:CDS 40.0%
!! TGCTTTTTGTCCTTTCTCCA+AGG + chr7.3:35174810-35174829 None:intergenic 40.0%
!!! GATTTTTCAGGCTGCTTTTC+AGG - chr7.3:35174964-35174983 MS.gene071014:CDS 40.0%
AAAATTCTCCCTGTGGTGCA+TGG - chr7.3:35174660-35174679 MS.gene071014:CDS 45.0%
AAGAGAGTCACCAATAGGAG+TGG + chr7.3:35174873-35174892 None:intergenic 45.0%
AGTGAGTCTTCCACACTTGA+TGG - chr7.3:35174769-35174788 MS.gene071014:CDS 45.0%
CCTCGGAGACAGTTGTAAAA+TGG - chr7.3:35174604-35174623 MS.gene071014:CDS 45.0%
CCTTCACAAAATTCTCCCTG+TGG - chr7.3:35174653-35174672 MS.gene071014:CDS 45.0%
CGATCTTCCTCATATGGGAA+TGG - chr7.3:35175369-35175388 MS.gene071014:CDS 45.0%
GATGACCGATCTTCCTCATA+TGG - chr7.3:35175363-35175382 MS.gene071014:CDS 45.0%
GGGTATAGTTCAAATCCCCT+CGG - chr7.3:35174587-35174606 MS.gene071014:CDS 45.0%
GTCATCAACATGGTCAACCA+TGG + chr7.3:35174237-35174256 None:intergenic 45.0%
GTCTCAAGAGAGTCACCAAT+AGG + chr7.3:35174878-35174897 None:intergenic 45.0%
TCAAGTGTGGAAGACTCACT+TGG + chr7.3:35174769-35174788 None:intergenic 45.0%
! ATGATCATAAGTGGCACTGG+CGG + chr7.3:35174630-35174649 None:intergenic 45.0%
! CCACAGGGAGAATTTTGTGA+AGG + chr7.3:35174656-35174675 None:intergenic 45.0%
! CCATTTTACAACTGTCTCCG+AGG + chr7.3:35174607-35174626 None:intergenic 45.0%
!!! GGGCGGAAAGAAAGTGTTTT+TGG - chr7.3:35175330-35175349 MS.gene071014:CDS 45.0%
CATGGTGAAGCCTTGGAGAA+AGG - chr7.3:35174797-35174816 MS.gene071014:CDS 50.0%
CCATGTGCTTCCATCAAGTG+TGG + chr7.3:35174782-35174801 None:intergenic 50.0%
CTATACCCTTCGAGGTCATG+TGG + chr7.3:35174575-35174594 None:intergenic 50.0%
TCTTGTATGCCTCGCCTTTG+AGG + chr7.3:35174946-35174965 None:intergenic 50.0%
TGAGCCGTATAGCTTCACCA+TGG - chr7.3:35174217-35174236 MS.gene071014:CDS 50.0%
TGGAGATTACCTCAAAGGCG+AGG - chr7.3:35174934-35174953 MS.gene071014:CDS 50.0%
! CCACACTTGATGGAAGCACA+TGG - chr7.3:35174779-35174798 MS.gene071014:CDS 50.0%
GTTGGACTCCATGCACCACA+GGG + chr7.3:35174671-35174690 None:intergenic 55.0%
TGTTGGACTCCATGCACCAC+AGG + chr7.3:35174672-35174691 None:intergenic 55.0%
! GGAAGCACATGGTGAAGCCT+TGG - chr7.3:35174790-35174809 MS.gene071014:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 35174122 35175885 35174122 ID=MS.gene071014
chr7.3 mRNA 35174122 35175885 35174122 ID=MS.gene071014.t1;Parent=MS.gene071014
chr7.3 exon 35174122 35175885 35174122 ID=MS.gene071014.t1.exon1;Parent=MS.gene071014.t1
chr7.3 CDS 35174122 35175885 35174122 ID=cds.MS.gene071014.t1;Parent=MS.gene071014.t1
Gene Sequence

>MS.gene071014

ATGAGTCAGAAACAGATTCTTCACTCTCCATGGCCTTTAATCTATGAACACATAGACTCATCAACCTTTGATCATCATCATCATCATCAAGTTGTTGAGCCGTATAGCTTCACCATGGTTGACCATGTTGATGACTATGATCCATATGGCATCATCATGGATGACCATGTTGATGATGACTATGTTCCATATGGCATCATCATGGATGATCATGTTGAGTATGATTATGAATTCAATACTCTATTAACCACTTCAGAGAATAGTACTCTCTCTGAAATCTCCATATTCACCAATGATCATATCCAATTTCCAATTGATGAAGAAACCTTGGAACTTCCATCTCTAATGGAATTAGAATCTTTTGATTCCATTTTGAGTCCACAAATTGGTAATATTCAAGAACATGAATACCAAGAAGAAAGTGAAGGGAGTTTCCCTTCACAAAATTTTCCACATGACCTCGAAGGGTATAGTTCAAATCCCCTCGGAGACAGTTGTAAAATGGCCGCCAGTGCCACTTATGATCATTTCCCTTCACAAAATTCTCCCTGTGGTGCATGGAGTCCAACACTTTCCATGAAATCAGATTTTTCATCAATTCAACAATCACTTACTTTGCCACAAGAAAATATGGAAATTGAAAACCAAGTGAGTCTTCCACACTTGATGGAAGCACATGGTGAAGCCTTGGAGAAAGGACAAAAAGCACTAGCAGAAGTTAATTTAAAGTGTATAAGCCAAAAAGTCACCACTCCTATTGGTGACTCTCTTGAGACACTTGCATATTACTTGGCTCAAGTAGTGACAAATCATGGAGATTACCTCAAAGGCGAGGCATACAAGATTTTTCAGGCTGCTTTTCAGGTATTTTATCAAGGGAATTTGGTTGGGAAAATTGCTCATTTTTCAGCTATTTCATCAATTCTTGAAGCTGCTATGTTAGAAGATTGTGATGAAATTCACATAATTGATTATTGTTTTGGAAATGGTGTTCAATGGCCTTTTCTATTGGAGACTGTTTCAAAAATGAATAAAAAATTGAAATTGACATCAATTAAATGGAGGGATGAAAATTCTGCATCTGTTTGGAATTTCGAGGATACCAAAAGGGAACTTTATGAGTATGCCAAATCATGTGGTTTGAAATTGAAGGTAGAGGAAAAGGAATTGGAAGAATTGATTAGTGAGATCAAAATAATGAACGAAAAGGGCGGAAAGAAAGTGTTTTTGGCTTTCAATTTGATGACCGATCTTCCTCATATGGGAATGGTTAGAAGTAGAAGAAAACATGCTTATGAGTTTTTGAAGGTAGCTGAGGATTTGATCAAGAACTATTGTAGGAAGGGAATTGTTACTTTTGGTGATGGTGATGCATTTGAGAAACTTAAAAATAGTTTGAATTTCAAGGCATTTTTTGAAGGGAATTTGTTGCATTATAAAGCTTTGTTGGAATCAATTGAGTCACAATTTTCAAAAAAATTCTCAGAAGCAAGAATTGAGTGTGAAGTGTTGTTTGTGGCACCTTGTATATCTTCTCTTGATTGGTTACAAACATGGGAAGAGATGAAAAGTGATGGTTATGTTAAAGATGAGATTAGCATAGAAGGTGGAAGATTGAGCAAAAATGTTTTGATGGAAGTCAGAGAAATGTTGAGAGGAAGTGATAGTTCATATCAAGCAAGAATTGAAGGAGAAAATGAGAATCAACTTGTTTTGGAATGGAAAGGAAGTCAACTGTTAAGATTTTCTATTTGGAAAAATTGA
Protein sequence

>MS.gene071014.t1

MSQKQILHSPWPLIYEHIDSSTFDHHHHHQVVEPYSFTMVDHVDDYDPYGIIMDDHVDDDYVPYGIIMDDHVEYDYEFNTLLTTSENSTLSEISIFTNDHIQFPIDEETLELPSLMELESFDSILSPQIGNIQEHEYQEESEGSFPSQNFPHDLEGYSSNPLGDSCKMAASATYDHFPSQNSPCGAWSPTLSMKSDFSSIQQSLTLPQENMEIENQVSLPHLMEAHGEALEKGQKALAEVNLKCISQKVTTPIGDSLETLAYYLAQVVTNHGDYLKGEAYKIFQAAFQVFYQGNLVGKIAHFSAISSILEAAMLEDCDEIHIIDYCFGNGVQWPFLLETVSKMNKKLKLTSIKWRDENSASVWNFEDTKRELYEYAKSCGLKLKVEEKELEELISEIKIMNEKGGKKVFLAFNLMTDLPHMGMVRSRRKHAYEFLKVAEDLIKNYCRKGIVTFGDGDAFEKLKNSLNFKAFFEGNLLHYKALLESIESQFSKKFSEARIECEVLFVAPCISSLDWLQTWEEMKSDGYVKDEISIEGGRLSKNVLMEVREMLRGSDSSYQARIEGENENQLVLEWKGSQLLRFSIWKN