AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071025


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071025.t1 MTR_1g013130 39.200 125 61 5 63 179 76 193 1.82e-12 66.2
MS.gene071025.t1 MTR_3g011990 43.678 87 32 3 67 146 72 148 1.95e-11 63.5
MS.gene071025.t1 MTR_3g012010 49.254 67 27 1 66 125 71 137 2.10e-11 63.5
MS.gene071025.t1 MTR_8g466760 46.667 75 27 3 77 148 86 150 3.81e-11 62.8
MS.gene071025.t1 MTR_7g026977 36.111 108 60 2 26 126 83 188 5.97e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071025.t1 AT5G10970 39.837 123 48 6 63 179 84 186 1.31e-11 63.9
MS.gene071025.t1 AT5G25160 41.837 98 48 4 82 179 57 145 1.74e-11 63.2
MS.gene071025.t1 AT1G80730 68.293 41 11 1 78 118 60 98 5.28e-11 61.6

Find 81 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGACCAATGCCTTTCCTTCT+TGG 0.138422 7.3:+34944013 MS.gene071025:CDS
TTTGATTTCGACTCTAATTT+TGG 0.159297 7.3:-34943601 None:intergenic
TGAATTATGCCTTCTCTATT+AGG 0.184623 7.3:-34944288 None:intergenic
CCCCAAGCAACAACACAATT+TGG 0.187401 7.3:+34944123 MS.gene071025:CDS
TCTTGGCTCGGTTCACCTTA+TGG 0.231125 7.3:+34944030 MS.gene071025:CDS
TGGTGTGAGTGCCAAGTCTT+AGG 0.231168 7.3:-34943681 None:intergenic
CGGGTTGCAAAGGGTGTATT+TGG 0.256836 7.3:-34944075 None:intergenic
CCCAAATTGTGTTGTTGCTT+GGG 0.273537 7.3:-34944124 None:intergenic
GTTGTTGCTTGGGGTGAATT+TGG 0.275394 7.3:-34944114 None:intergenic
CCCAAAGTGTTTGACCTTTG+TGG 0.286115 7.3:-34944192 None:intergenic
GTTCACGCTTATGAGCATTT+TGG 0.317761 7.3:-34943852 None:intergenic
AGGTCAAACACTTTGGGTTT+GGG 0.338136 7.3:+34944198 MS.gene071025:CDS
CCACAAAGGTCAAACACTTT+GGG 0.340923 7.3:+34944192 MS.gene071025:CDS
GTGTTTGACCTTTGTGGAAT+TGG 0.343209 7.3:-34944186 None:intergenic
AAGGTCAAACACTTTGGGTT+TGG 0.344659 7.3:+34944197 MS.gene071025:CDS
CAAGGGTTTGGAAGTGGACT+TGG 0.360530 7.3:-34943701 None:intergenic
GTCACATATTGGACGCCAAT+TGG 0.375665 7.3:-34943994 None:intergenic
TCCCAAATTGTGTTGTTGCT+TGG 0.377648 7.3:-34944125 None:intergenic
TTCTCCACTTCTCAAGCTCT+TGG 0.384550 7.3:+34943823 MS.gene071025:CDS
TAGAAACCTCAAACGCAAAA+TGG 0.385914 7.3:+34943570 MS.gene071025:CDS
TCCACCGTTCTCCAAGGGTT+TGG 0.389944 7.3:-34943713 None:intergenic
CACCTTATGGTGGCTATGGA+GGG 0.398816 7.3:+34944043 MS.gene071025:CDS
GAAAGGCATTGGTCACATAT+TGG 0.406502 7.3:-34944005 None:intergenic
TAATAGAGAAGGCATAATTC+AGG 0.407241 7.3:+34944290 MS.gene071025:CDS
CCCAAGCAACAACACAATTT+GGG 0.410223 7.3:+34944124 MS.gene071025:CDS
TCCACAAAGGTCAAACACTT+TGG 0.411221 7.3:+34944191 MS.gene071025:CDS
TTAGGATCACGAGATAATAA+TGG 0.420098 7.3:-34944270 None:intergenic
ACCACTTTCTGCTCAGGTAA+AGG 0.442903 7.3:-34943769 None:intergenic
TCAAGCTCTATTCACTATCA+AGG 0.445678 7.3:+34943937 MS.gene071025:CDS
AATGCCTTTCCTTCTTGGCT+CGG 0.453136 7.3:+34944018 MS.gene071025:CDS
TTGGCATTTGCATCACATAC+CGG 0.471216 7.3:-34944095 None:intergenic
GTTCTCCAAGGGTTTGGAAG+TGG 0.473154 7.3:-34943707 None:intergenic
TCACATATTGGACGCCAATT+GGG 0.475144 7.3:-34943993 None:intergenic
TCACCTTATGGTGGCTATGG+AGG 0.475493 7.3:+34944042 MS.gene071025:CDS
TCCACTTCTCAAGCTCTTGG+AGG 0.477317 7.3:+34943826 MS.gene071025:CDS
GCTAACTTGCACAACCCAAT+TGG 0.486494 7.3:+34943979 MS.gene071025:CDS
GGTGTGAGTGCCAAGTCTTA+GGG 0.491546 7.3:-34943680 None:intergenic
TAAAAGGAGAGAGGAAGAGA+TGG 0.501837 7.3:+34943897 MS.gene071025:CDS
GGTTCACCTTATGGTGGCTA+TGG 0.502207 7.3:+34944039 MS.gene071025:CDS
AACTCTACCACTTTCTGCTC+AGG 0.502340 7.3:-34943775 None:intergenic
CCAAATTGTGTTGTTGCTTG+GGG 0.502858 7.3:-34944123 None:intergenic
AGTTGATGATGAGGAAACAT+TGG 0.505069 7.3:-34944319 None:intergenic
CAAGTCCACTTCCAAACCCT+TGG 0.519689 7.3:+34943702 MS.gene071025:CDS
GTTGATGATGAGGAAACATT+GGG 0.520842 7.3:-34944318 None:intergenic
TGATCAATGAAATTTCTTCG+AGG 0.520979 7.3:-34944228 None:intergenic
ATCACATACCGGGTTGCAAA+GGG 0.522044 7.3:-34944084 None:intergenic
ACCCCTCCATAGCCACCATA+AGG 0.522093 7.3:-34944045 None:intergenic
TGGCATTTGCATCACATACC+GGG 0.531856 7.3:-34944094 None:intergenic
TCTATCCTCATCATTTCCAA+TGG 0.532227 7.3:-34943551 None:intergenic
ATTTCCAATGGAAATTCTCT+CGG 0.535410 7.3:-34943539 None:intergenic
ACCTTATGGTGGCTATGGAG+GGG 0.537456 7.3:+34944044 MS.gene071025:CDS
ATTAGAGTCGAAATCAAAGA+AGG 0.542585 7.3:+34943606 MS.gene071025:CDS
CAAATACACCCTTTGCAACC+CGG 0.543155 7.3:+34944076 MS.gene071025:CDS
GAATAGAGCTTGAAAACCAT+AGG 0.547197 7.3:-34943927 None:intergenic
TCAACATCAGATTCAGAGGT+TGG 0.549087 7.3:-34943646 None:intergenic
TAGCACTACGGAAATATGAA+TGG 0.550571 7.3:-34943960 None:intergenic
AACCTCTGAATCTGATGTTG+AGG 0.555993 7.3:+34943648 MS.gene071025:CDS
ACCTCCAAGAGCTTGAGAAG+TGG 0.556669 7.3:-34943827 None:intergenic
AATGGAAGATAAAAGGAGAG+AGG 0.557828 7.3:+34943888 MS.gene071025:CDS
TGAATGTCCACCGTTCTCCA+AGG 0.561681 7.3:-34943719 None:intergenic
CATCACATACCGGGTTGCAA+AGG 0.564146 7.3:-34944085 None:intergenic
AAGAGATGGATTCAACCCTA+TGG 0.564603 7.3:+34943911 MS.gene071025:CDS
GAATGTCCACCGTTCTCCAA+GGG 0.565536 7.3:-34943718 None:intergenic
ACCTTTACCTGAGCAGAAAG+TGG 0.574184 7.3:+34943768 MS.gene071025:CDS
TAAGGTGAACCGAGCCAAGA+AGG 0.575815 7.3:-34944027 None:intergenic
TGAACCGAGCCAAGAAGGAA+AGG 0.582959 7.3:-34944022 None:intergenic
GAGCCAAGAAGGAAAGGCAT+TGG 0.584408 7.3:-34944016 None:intergenic
ATGTCCGAGAGAATTTCCAT+TGG 0.586763 7.3:+34943535 MS.gene071025:CDS
AATAGAGCTTGAAAACCATA+GGG 0.593787 7.3:-34943926 None:intergenic
ATCCTCAACATCAGATTCAG+AGG 0.595365 7.3:-34943650 None:intergenic
TCTCGTGATCCTAATAGAGA+AGG 0.597683 7.3:+34944279 MS.gene071025:CDS
AATTTCCATTGGAAATGATG+AGG 0.604935 7.3:+34943546 MS.gene071025:CDS
CCTCAAACGCAAAATGGTGG+AGG 0.605406 7.3:+34943576 MS.gene071025:CDS
GATTTACTAACCCTAAGACT+TGG 0.606458 7.3:+34943670 MS.gene071025:CDS
ACTTCCAAACCCTTGGAGAA+CGG 0.610472 7.3:+34943709 MS.gene071025:CDS
TGGCTCGGTTCACCTTATGG+TGG 0.610898 7.3:+34944033 MS.gene071025:CDS
AAACCTCAAACGCAAAATGG+TGG 0.616518 7.3:+34943573 MS.gene071025:CDS
GGTTGTGCAAGTTAGCACTA+CGG 0.648362 7.3:-34943972 None:intergenic
CGTTGATTGAGTTGATGATG+AGG 0.655857 7.3:-34944328 None:intergenic
TCCAAACCCTTGGAGAACGG+TGG 0.656704 7.3:+34943712 MS.gene071025:CDS
CTGCATTTCCAATTCCACAA+AGG 0.667747 7.3:+34944178 MS.gene071025:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAATGGAAGATAAA+AGG + chr7.3:34943881-34943900 MS.gene071025:CDS 15.0%
! TGAACGTGTCTTAAAAAAAA+TGG + chr7.3:34943870-34943889 MS.gene071025:CDS 25.0%
!!! AATTTGGGATGACTAATTTT+TGG + chr7.3:34944139-34944158 MS.gene071025:CDS 25.0%
!!! ATTTGGGATGACTAATTTTT+GGG + chr7.3:34944140-34944159 MS.gene071025:CDS 25.0%
!!! TTTGATTTCGACTCTAATTT+TGG - chr7.3:34943604-34943623 None:intergenic 25.0%
AATAGAGCTTGAAAACCATA+GGG - chr7.3:34943929-34943948 None:intergenic 30.0%
AATTTCCATTGGAAATGATG+AGG + chr7.3:34943546-34943565 MS.gene071025:CDS 30.0%
ATTAGAGTCGAAATCAAAGA+AGG + chr7.3:34943606-34943625 MS.gene071025:CDS 30.0%
ATTTCCAATGGAAATTCTCT+CGG - chr7.3:34943542-34943561 None:intergenic 30.0%
TGAATTATGCCTTCTCTATT+AGG - chr7.3:34944291-34944310 None:intergenic 30.0%
TGATCAATGAAATTTCTTCG+AGG - chr7.3:34944231-34944250 None:intergenic 30.0%
TTAGGATCACGAGATAATAA+TGG - chr7.3:34944273-34944292 None:intergenic 30.0%
! TAATAGAGAAGGCATAATTC+AGG + chr7.3:34944290-34944309 MS.gene071025:CDS 30.0%
!! TTTGGGATGACTAATTTTTG+GGG + chr7.3:34944141-34944160 MS.gene071025:CDS 30.0%
AATGGAAGATAAAAGGAGAG+AGG + chr7.3:34943888-34943907 MS.gene071025:CDS 35.0%
GAATAGAGCTTGAAAACCAT+AGG - chr7.3:34943930-34943949 None:intergenic 35.0%
GATTTACTAACCCTAAGACT+TGG + chr7.3:34943670-34943689 MS.gene071025:CDS 35.0%
TAGAAACCTCAAACGCAAAA+TGG + chr7.3:34943570-34943589 MS.gene071025:CDS 35.0%
TAGCACTACGGAAATATGAA+TGG - chr7.3:34943963-34943982 None:intergenic 35.0%
TCAAGCTCTATTCACTATCA+AGG + chr7.3:34943937-34943956 MS.gene071025:CDS 35.0%
TCTATCCTCATCATTTCCAA+TGG - chr7.3:34943554-34943573 None:intergenic 35.0%
!! AGTTGATGATGAGGAAACAT+TGG - chr7.3:34944322-34944341 None:intergenic 35.0%
!! GTTGATGATGAGGAAACATT+GGG - chr7.3:34944321-34944340 None:intergenic 35.0%
AAACCTCAAACGCAAAATGG+TGG + chr7.3:34943573-34943592 MS.gene071025:CDS 40.0%
AACCTCTGAATCTGATGTTG+AGG + chr7.3:34943648-34943667 MS.gene071025:CDS 40.0%
ATCCTCAACATCAGATTCAG+AGG - chr7.3:34943653-34943672 None:intergenic 40.0%
ATGTCCGAGAGAATTTCCAT+TGG + chr7.3:34943535-34943554 MS.gene071025:CDS 40.0%
CCACAAAGGTCAAACACTTT+GGG + chr7.3:34944192-34944211 MS.gene071025:CDS 40.0%
CCCAAGCAACAACACAATTT+GGG + chr7.3:34944124-34944143 MS.gene071025:CDS 40.0%
CTGCATTTCCAATTCCACAA+AGG + chr7.3:34944178-34944197 MS.gene071025:CDS 40.0%
GTGTTTGACCTTTGTGGAAT+TGG - chr7.3:34944189-34944208 None:intergenic 40.0%
TAAAAGGAGAGAGGAAGAGA+TGG + chr7.3:34943897-34943916 MS.gene071025:CDS 40.0%
TCAACATCAGATTCAGAGGT+TGG - chr7.3:34943649-34943668 None:intergenic 40.0%
TCACATATTGGACGCCAATT+GGG - chr7.3:34943996-34944015 None:intergenic 40.0%
TCCACAAAGGTCAAACACTT+TGG + chr7.3:34944191-34944210 MS.gene071025:CDS 40.0%
TCTCGTGATCCTAATAGAGA+AGG + chr7.3:34944279-34944298 MS.gene071025:CDS 40.0%
TTGGCATTTGCATCACATAC+CGG - chr7.3:34944098-34944117 None:intergenic 40.0%
! AAGAGATGGATTCAACCCTA+TGG + chr7.3:34943911-34943930 MS.gene071025:CDS 40.0%
! AAGGTCAAACACTTTGGGTT+TGG + chr7.3:34944197-34944216 MS.gene071025:CDS 40.0%
! AGGTCAAACACTTTGGGTTT+GGG + chr7.3:34944198-34944217 MS.gene071025:CDS 40.0%
! CCAAATTGTGTTGTTGCTTG+GGG - chr7.3:34944126-34944145 None:intergenic 40.0%
! CCCAAATTGTGTTGTTGCTT+GGG - chr7.3:34944127-34944146 None:intergenic 40.0%
! GTTCACGCTTATGAGCATTT+TGG - chr7.3:34943855-34943874 None:intergenic 40.0%
! TCCCAAATTGTGTTGTTGCT+TGG - chr7.3:34944128-34944147 None:intergenic 40.0%
!! CGTTGATTGAGTTGATGATG+AGG - chr7.3:34944331-34944350 None:intergenic 40.0%
!! GAAAGGCATTGGTCACATAT+TGG - chr7.3:34944008-34944027 None:intergenic 40.0%
!!! ATGACTAATTTTTGGGGTGG+AGG + chr7.3:34944147-34944166 MS.gene071025:CDS 40.0%
AACTCTACCACTTTCTGCTC+AGG - chr7.3:34943778-34943797 None:intergenic 45.0%
AATGCCTTTCCTTCTTGGCT+CGG + chr7.3:34944018-34944037 MS.gene071025:CDS 45.0%
ACCACTTTCTGCTCAGGTAA+AGG - chr7.3:34943772-34943791 None:intergenic 45.0%
ACCTTTACCTGAGCAGAAAG+TGG + chr7.3:34943768-34943787 MS.gene071025:CDS 45.0%
ACTTCCAAACCCTTGGAGAA+CGG + chr7.3:34943709-34943728 MS.gene071025:CDS 45.0%
ATCACATACCGGGTTGCAAA+GGG - chr7.3:34944087-34944106 None:intergenic 45.0%
CAAATACACCCTTTGCAACC+CGG + chr7.3:34944076-34944095 MS.gene071025:CDS 45.0%
CCCCAAGCAACAACACAATT+TGG + chr7.3:34944123-34944142 MS.gene071025:CDS 45.0%
GGTTGTGCAAGTTAGCACTA+CGG - chr7.3:34943975-34943994 None:intergenic 45.0%
GTCACATATTGGACGCCAAT+TGG - chr7.3:34943997-34944016 None:intergenic 45.0%
TGACCAATGCCTTTCCTTCT+TGG + chr7.3:34944013-34944032 MS.gene071025:CDS 45.0%
TGGCATTTGCATCACATACC+GGG - chr7.3:34944097-34944116 None:intergenic 45.0%
TTCTCCACTTCTCAAGCTCT+TGG + chr7.3:34943823-34943842 MS.gene071025:CDS 45.0%
! CCCAAAGTGTTTGACCTTTG+TGG - chr7.3:34944195-34944214 None:intergenic 45.0%
! GCTAACTTGCACAACCCAAT+TGG + chr7.3:34943979-34943998 MS.gene071025:CDS 45.0%
! GTTGTTGCTTGGGGTGAATT+TGG - chr7.3:34944117-34944136 None:intergenic 45.0%
!! GGGATGACTAATTTTTGGGG+TGG + chr7.3:34944144-34944163 MS.gene071025:CDS 45.0%
ACCTCCAAGAGCTTGAGAAG+TGG - chr7.3:34943830-34943849 None:intergenic 50.0%
ACCTTATGGTGGCTATGGAG+GGG + chr7.3:34944044-34944063 MS.gene071025:CDS 50.0%
CAAGTCCACTTCCAAACCCT+TGG + chr7.3:34943702-34943721 MS.gene071025:CDS 50.0%
CACCTTATGGTGGCTATGGA+GGG + chr7.3:34944043-34944062 MS.gene071025:CDS 50.0%
CATCACATACCGGGTTGCAA+AGG - chr7.3:34944088-34944107 None:intergenic 50.0%
CCTCAAACGCAAAATGGTGG+AGG + chr7.3:34943576-34943595 MS.gene071025:CDS 50.0%
GAATGTCCACCGTTCTCCAA+GGG - chr7.3:34943721-34943740 None:intergenic 50.0%
GGTGTGAGTGCCAAGTCTTA+GGG - chr7.3:34943683-34943702 None:intergenic 50.0%
GGTTCACCTTATGGTGGCTA+TGG + chr7.3:34944039-34944058 MS.gene071025:CDS 50.0%
GTTCTCCAAGGGTTTGGAAG+TGG - chr7.3:34943710-34943729 None:intergenic 50.0%
TAAGGTGAACCGAGCCAAGA+AGG - chr7.3:34944030-34944049 None:intergenic 50.0%
TCACCTTATGGTGGCTATGG+AGG + chr7.3:34944042-34944061 MS.gene071025:CDS 50.0%
TCCACTTCTCAAGCTCTTGG+AGG + chr7.3:34943826-34943845 MS.gene071025:CDS 50.0%
TGAACCGAGCCAAGAAGGAA+AGG - chr7.3:34944025-34944044 None:intergenic 50.0%
TGAATGTCCACCGTTCTCCA+AGG - chr7.3:34943722-34943741 None:intergenic 50.0%
TGGTGTGAGTGCCAAGTCTT+AGG - chr7.3:34943684-34943703 None:intergenic 50.0%
! CAAGGGTTTGGAAGTGGACT+TGG - chr7.3:34943704-34943723 None:intergenic 50.0%
! CCTCCACCATTTTGCGTTTG+AGG - chr7.3:34943579-34943598 None:intergenic 50.0%
! CGGGTTGCAAAGGGTGTATT+TGG - chr7.3:34944078-34944097 None:intergenic 50.0%
! GAGCCAAGAAGGAAAGGCAT+TGG - chr7.3:34944019-34944038 None:intergenic 50.0%
!! TCTTGGCTCGGTTCACCTTA+TGG + chr7.3:34944030-34944049 MS.gene071025:CDS 50.0%
ACCCCTCCATAGCCACCATA+AGG - chr7.3:34944048-34944067 None:intergenic 55.0%
TCCAAACCCTTGGAGAACGG+TGG + chr7.3:34943712-34943731 MS.gene071025:CDS 55.0%
TCCACCGTTCTCCAAGGGTT+TGG - chr7.3:34943716-34943735 None:intergenic 55.0%
!! TGGCTCGGTTCACCTTATGG+TGG + chr7.3:34944033-34944052 MS.gene071025:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 34943535 34944353 34943535 ID=MS.gene071025
chr7.3 mRNA 34943535 34944353 34943535 ID=MS.gene071025.t1;Parent=MS.gene071025
chr7.3 exon 34943535 34944353 34943535 ID=MS.gene071025.t1.exon1;Parent=MS.gene071025.t1
chr7.3 CDS 34943535 34944353 34943535 ID=cds.MS.gene071025.t1;Parent=MS.gene071025.t1
Gene Sequence

>MS.gene071025

ATGTCCGAGAGAATTTCCATTGGAAATGATGAGGATAGAAACCTCAAACGCAAAATGGTGGAGGAGCCAAAATTAGAGTCGAAATCAAAGAAGGAGTCAAAGACAATGACACCAACCTCTGAATCTGATGTTGAGGATTTACTAACCCTAAGACTTGGCACTCACACCAAGTCCACTTCCAAACCCTTGGAGAACGGTGGACATTCAAGTTCATTTCAAAACACTACTGATGAACCTTTACCTGAGCAGAAAGTGGTAGAGTTCTCTTGTAATTATTGTGACAAGAAATTCTCCACTTCTCAAGCTCTTGGAGGTCACCAAAATGCTCATAAGCGTGAACGTGTCTTAAAAAAAATGGAAGATAAAAGGAGAGAGGAAGAGATGGATTCAACCCTATGGTTTTCAAGCTCTATTCACTATCAAGGCCATTCATATTTCCGTAGTGCTAACTTGCACAACCCAATTGGCGTCCAATATGTGACCAATGCCTTTCCTTCTTGGCTCGGTTCACCTTATGGTGGCTATGGAGGGGTGTATATGCCAAATACACCCTTTGCAACCCGGTATGTGATGCAAATGCCAAATTCACCCCAAGCAACAACACAATTTGGGATGACTAATTTTTGGGGTGGAGGTCAAAATGCTGCATTTCCAATTCCACAAAGGTCAAACACTTTGGGTTTGGGACTTTTTCCTCGAAGAAATTTCATTGATCAACTTCGTTCTCATACTCTTCCATTATTATCTCGTGATCCTAATAGAGAAGGCATAATTCAGGCAAATCCCAATGTTTCCTCATCATCAACTCAATCAACGTAG

Protein sequence

>MS.gene071025.t1

MSERISIGNDEDRNLKRKMVEEPKLESKSKKESKTMTPTSESDVEDLLTLRLGTHTKSTSKPLENGGHSSSFQNTTDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHKRERVLKKMEDKRREEEMDSTLWFSSSIHYQGHSYFRSANLHNPIGVQYVTNAFPSWLGSPYGGYGGVYMPNTPFATRYVMQMPNSPQATTQFGMTNFWGGGQNAAFPIPQRSNTLGLGLFPRRNFIDQLRSHTLPLLSRDPNREGIIQANPNVSSSSTQST