AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071026


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071026.t1 MTR_1g013130 35.374 147 67 6 63 209 76 194 4.60e-12 65.1
MS.gene071026.t1 MTR_3g011990 45.977 87 30 4 67 146 72 148 1.56e-11 63.9
MS.gene071026.t1 MTR_3g012010 49.254 67 27 1 66 125 71 137 1.85e-11 63.5
MS.gene071026.t1 MTR_8g466760 46.667 75 27 3 77 148 86 150 3.33e-11 62.8
MS.gene071026.t1 MTR_7g026977 36.111 108 60 2 26 126 83 188 5.32e-11 62.4
MS.gene071026.t1 MTR_3g065020 71.795 39 9 1 80 118 82 118 8.22e-11 60.8
MS.gene071026.t1 MTR_4g006575 31.034 145 80 4 88 228 69 197 8.58e-11 60.8
MS.gene071026.t1 MTR_4g006600 31.034 145 80 4 88 228 69 197 8.58e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071026.t1 AT5G10970 41.935 124 43 7 63 178 84 186 4.58e-12 65.1
MS.gene071026.t1 AT5G25160 40.206 97 50 3 82 178 57 145 4.53e-11 61.6
MS.gene071026.t1 AT1G80730 68.293 41 11 1 78 118 60 98 4.73e-11 61.6

Find 80 sgRNAs with CRISPR-Local

Find 88 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTTGATTTCGACTCTAATTT+TGG 0.159297 7.3:-34939581 None:intergenic
TGAATTATGCCTTCTCTATT+AGG 0.177692 7.3:-34940265 None:intergenic
CCCCAAGCAACAACACAATT+TGG 0.187401 7.3:+34940100 MS.gene071026:CDS
TGGTGTGAGTGCCAAGTCTT+AGG 0.231168 7.3:-34939661 None:intergenic
CCCAAATTGTGTTGTTGCTT+GGG 0.273537 7.3:-34940101 None:intergenic
GTTGTTGCTTGGGGTGAATT+TGG 0.275394 7.3:-34940091 None:intergenic
CCCAAAGTGTTTGACCTTTG+TGG 0.277762 7.3:-34940169 None:intergenic
AGGTCAAACACTTTGGGTTT+TGG 0.290151 7.3:+34940175 MS.gene071026:CDS
CAGGTTGCAAGGGGTGTATT+TGG 0.297330 7.3:-34940052 None:intergenic
GTTCACGCTTATGAGCATTT+TGG 0.317761 7.3:-34939832 None:intergenic
CCACAAAGGTCAAACACTTT+GGG 0.340923 7.3:+34940169 MS.gene071026:CDS
TTGGTCACATGGGCGCCAAT+TGG 0.347934 7.3:-34939974 None:intergenic
CAAGGGTTTGGAAGTGGACT+TGG 0.360530 7.3:-34939681 None:intergenic
GTGTTTGACCTTTGTGGAAT+TGG 0.364677 7.3:-34940163 None:intergenic
TCCCAAATTGTGTTGTTGCT+TGG 0.377648 7.3:-34940102 None:intergenic
TTCTCCACTTCTCAAGCTCT+TGG 0.384550 7.3:+34939803 MS.gene071026:CDS
TAGAAACCTCAAACGCAAAA+TGG 0.385914 7.3:+34939550 MS.gene071026:CDS
TCCACCGTTCTCCAAGGGTT+TGG 0.389944 7.3:-34939693 None:intergenic
GCTTGGCTCGGTTCACCATA+TGG 0.400606 7.3:+34940007 MS.gene071026:CDS
TAATAGAGAAGGCATAATTC+AGG 0.407241 7.3:+34940267 MS.gene071026:CDS
CCCAAGCAACAACACAATTT+GGG 0.410223 7.3:+34940101 MS.gene071026:CDS
TCCACAAAGGTCAAACACTT+TGG 0.411221 7.3:+34940168 MS.gene071026:CDS
CACCATATGGTGGCTATGGA+GGG 0.414143 7.3:+34940020 MS.gene071026:CDS
TGACCAATGTCTTTCCTGCT+TGG 0.417465 7.3:+34939990 MS.gene071026:CDS
TTAGGATCACGAGATAATAA+TGG 0.420098 7.3:-34940247 None:intergenic
TGGTCACATGGGCGCCAATT+GGG 0.428506 7.3:-34939973 None:intergenic
ACCACTTTCTGCTCAGGTAA+AGG 0.442903 7.3:-34939749 None:intergenic
TCAAGCTCTATTCACTATCA+AGG 0.445678 7.3:+34939917 MS.gene071026:CDS
AATGTCTTTCCTGCTTGGCT+CGG 0.451878 7.3:+34939995 MS.gene071026:CDS
TGATCAATGAAATTTCTTTG+AGG 0.466939 7.3:-34940205 None:intergenic
GTTCTCCAAGGGTTTGGAAG+TGG 0.473154 7.3:-34939687 None:intergenic
TCCACTTCTCAAGCTCTTGG+AGG 0.477317 7.3:+34939806 MS.gene071026:CDS
TCACCATATGGTGGCTATGG+AGG 0.477975 7.3:+34940019 MS.gene071026:CDS
GCTAACTTGCACAACCCAAT+TGG 0.486494 7.3:+34939959 MS.gene071026:CDS
ACCCCTCCATAGCCACCATA+TGG 0.490038 7.3:-34940022 None:intergenic
GGTGTGAGTGCCAAGTCTTA+GGG 0.491546 7.3:-34939660 None:intergenic
GGTTCACCATATGGTGGCTA+TGG 0.499351 7.3:+34940016 MS.gene071026:CDS
TAAAAGGAGAGAGGAAGAGA+TGG 0.501837 7.3:+34939877 MS.gene071026:CDS
AACTCTACCACTTTCTGCTC+AGG 0.502340 7.3:-34939755 None:intergenic
CCAAATTGTGTTGTTGCTTG+GGG 0.502858 7.3:-34940100 None:intergenic
CAAATACACCCCTTGCAACC+TGG 0.503232 7.3:+34940053 MS.gene071026:CDS
TGGCATTTGCATCACATACC+AGG 0.518397 7.3:-34940071 None:intergenic
CAAGTCCACTTCCAAACCCT+TGG 0.519689 7.3:+34939682 MS.gene071026:CDS
GTTGATGATGAGGACACATT+GGG 0.531489 7.3:-34940295 None:intergenic
TCTATCCTCATCATTTCCAA+TGG 0.532227 7.3:-34939531 None:intergenic
CAGGAAAGACATTGGTCACA+TGG 0.533199 7.3:-34939985 None:intergenic
ATTTCCAATGGAAATTCTCT+CGG 0.535410 7.3:-34939519 None:intergenic
TATGGTGAACCGAGCCAAGC+AGG 0.538944 7.3:-34940004 None:intergenic
GCATCACATACCAGGTTGCA+AGG 0.542521 7.3:-34940063 None:intergenic
ATTAGAGTCGAAATCAAAGA+AGG 0.542585 7.3:+34939586 MS.gene071026:CDS
GAATAGAGCTTGAAAACCAT+AGG 0.547197 7.3:-34939907 None:intergenic
TCAACATCAGATTCAGAGGT+TGG 0.549087 7.3:-34939626 None:intergenic
TAGCACTACGGAAATATGAA+TGG 0.550571 7.3:-34939940 None:intergenic
ACCATATGGTGGCTATGGAG+GGG 0.551080 7.3:+34940021 MS.gene071026:CDS
AACCTCTGAATCTGATGTTG+AGG 0.555993 7.3:+34939628 MS.gene071026:CDS
ACCTCCAAGAGCTTGAGAAG+TGG 0.556669 7.3:-34939807 None:intergenic
AATGGAAGATAAAAGGAGAG+AGG 0.557828 7.3:+34939868 MS.gene071026:CDS
TGAATGTCCACCGTTCTCCA+AGG 0.561681 7.3:-34939699 None:intergenic
AAGAGATGGATTCAACCCTA+TGG 0.564603 7.3:+34939891 MS.gene071026:CDS
GAATGTCCACCGTTCTCCAA+GGG 0.565536 7.3:-34939698 None:intergenic
ACCTTTACCTGAGCAGAAAG+TGG 0.574184 7.3:+34939748 MS.gene071026:CDS
ATGTCCGAGAGAATTTCCAT+TGG 0.586763 7.3:+34939515 MS.gene071026:CDS
CATCACATACCAGGTTGCAA+GGG 0.588137 7.3:-34940062 None:intergenic
AATAGAGCTTGAAAACCATA+GGG 0.593787 7.3:-34939906 None:intergenic
ATCCTCAACATCAGATTCAG+AGG 0.595365 7.3:-34939630 None:intergenic
AGTTGATGATGAGGACACAT+TGG 0.595890 7.3:-34940296 None:intergenic
TCTCGTGATCCTAATAGAGA+AGG 0.597683 7.3:+34940256 MS.gene071026:CDS
AATTTCCATTGGAAATGATG+AGG 0.604935 7.3:+34939526 MS.gene071026:CDS
CCTCAAACGCAAAATGGTGG+AGG 0.605406 7.3:+34939556 MS.gene071026:CDS
GATTTACTAACCCTAAGACT+TGG 0.606458 7.3:+34939650 MS.gene071026:CDS
ACTTCCAAACCCTTGGAGAA+CGG 0.610472 7.3:+34939689 MS.gene071026:CDS
AAACCTCAAACGCAAAATGG+TGG 0.616518 7.3:+34939553 MS.gene071026:CDS
GGTTGTGCAAGTTAGCACTA+CGG 0.648362 7.3:-34939952 None:intergenic
TCCAAACCCTTGGAGAACGG+TGG 0.656704 7.3:+34939692 MS.gene071026:CDS
GAGCCAAGCAGGAAAGACAT+TGG 0.656849 7.3:-34939993 None:intergenic
ATCACATACCAGGTTGCAAG+GGG 0.662631 7.3:-34940061 None:intergenic
AGGAAAGACATTGGTCACAT+GGG 0.665048 7.3:-34939984 None:intergenic
TGGCTCGGTTCACCATATGG+TGG 0.670478 7.3:+34940010 MS.gene071026:CDS
GGTTGATTGAGTTGATGATG+AGG 0.677458 7.3:-34940305 None:intergenic
CTGCATTGCCAATTCCACAA+AGG 0.690145 7.3:+34940155 MS.gene071026:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAAAAAATGGAAGATAAA+AGG + chr7.3:34939861-34939880 MS.gene071026:CDS 15.0%
! TGAACGTGTCTTAAAAAAAA+TGG + chr7.3:34939850-34939869 MS.gene071026:CDS 25.0%
! TGATCAATGAAATTTCTTTG+AGG - chr7.3:34940208-34940227 None:intergenic 25.0%
!!! AATTTGGGATGACTAATTTT+TGG + chr7.3:34940116-34940135 MS.gene071026:CDS 25.0%
!!! ATTTGGGATGACTAATTTTT+GGG + chr7.3:34940117-34940136 MS.gene071026:CDS 25.0%
!!! TTTGATTTCGACTCTAATTT+TGG - chr7.3:34939584-34939603 None:intergenic 25.0%
AATAGAGCTTGAAAACCATA+GGG - chr7.3:34939909-34939928 None:intergenic 30.0%
AATTTCCATTGGAAATGATG+AGG + chr7.3:34939526-34939545 MS.gene071026:CDS 30.0%
ATTAGAGTCGAAATCAAAGA+AGG + chr7.3:34939586-34939605 MS.gene071026:CDS 30.0%
ATTTCCAATGGAAATTCTCT+CGG - chr7.3:34939522-34939541 None:intergenic 30.0%
TGAATTATGCCTTCTCTATT+AGG - chr7.3:34940268-34940287 None:intergenic 30.0%
TTAGGATCACGAGATAATAA+TGG - chr7.3:34940250-34940269 None:intergenic 30.0%
! TAATAGAGAAGGCATAATTC+AGG + chr7.3:34940267-34940286 MS.gene071026:CDS 30.0%
!! TTTGGGATGACTAATTTTTG+GGG + chr7.3:34940118-34940137 MS.gene071026:CDS 30.0%
AATGGAAGATAAAAGGAGAG+AGG + chr7.3:34939868-34939887 MS.gene071026:CDS 35.0%
GAATAGAGCTTGAAAACCAT+AGG - chr7.3:34939910-34939929 None:intergenic 35.0%
GATTTACTAACCCTAAGACT+TGG + chr7.3:34939650-34939669 MS.gene071026:CDS 35.0%
TAGAAACCTCAAACGCAAAA+TGG + chr7.3:34939550-34939569 MS.gene071026:CDS 35.0%
TAGCACTACGGAAATATGAA+TGG - chr7.3:34939943-34939962 None:intergenic 35.0%
TCAAGCTCTATTCACTATCA+AGG + chr7.3:34939917-34939936 MS.gene071026:CDS 35.0%
TCTATCCTCATCATTTCCAA+TGG - chr7.3:34939534-34939553 None:intergenic 35.0%
AAACCTCAAACGCAAAATGG+TGG + chr7.3:34939553-34939572 MS.gene071026:CDS 40.0%
AACCTCTGAATCTGATGTTG+AGG + chr7.3:34939628-34939647 MS.gene071026:CDS 40.0%
AGGAAAGACATTGGTCACAT+GGG - chr7.3:34939987-34940006 None:intergenic 40.0%
ATCCTCAACATCAGATTCAG+AGG - chr7.3:34939633-34939652 None:intergenic 40.0%
ATGTCCGAGAGAATTTCCAT+TGG + chr7.3:34939515-34939534 MS.gene071026:CDS 40.0%
CCACAAAGGTCAAACACTTT+GGG + chr7.3:34940169-34940188 MS.gene071026:CDS 40.0%
CCCAAGCAACAACACAATTT+GGG + chr7.3:34940101-34940120 MS.gene071026:CDS 40.0%
GTGTTTGACCTTTGTGGAAT+TGG - chr7.3:34940166-34940185 None:intergenic 40.0%
TAAAAGGAGAGAGGAAGAGA+TGG + chr7.3:34939877-34939896 MS.gene071026:CDS 40.0%
TCAACATCAGATTCAGAGGT+TGG - chr7.3:34939629-34939648 None:intergenic 40.0%
TCCACAAAGGTCAAACACTT+TGG + chr7.3:34940168-34940187 MS.gene071026:CDS 40.0%
TCTCGTGATCCTAATAGAGA+AGG + chr7.3:34940256-34940275 MS.gene071026:CDS 40.0%
! AAGAGATGGATTCAACCCTA+TGG + chr7.3:34939891-34939910 MS.gene071026:CDS 40.0%
! CCAAATTGTGTTGTTGCTTG+GGG - chr7.3:34940103-34940122 None:intergenic 40.0%
! CCCAAATTGTGTTGTTGCTT+GGG - chr7.3:34940104-34940123 None:intergenic 40.0%
! GTTCACGCTTATGAGCATTT+TGG - chr7.3:34939835-34939854 None:intergenic 40.0%
! TCCCAAATTGTGTTGTTGCT+TGG - chr7.3:34940105-34940124 None:intergenic 40.0%
!! AGGTCAAACACTTTGGGTTT+TGG + chr7.3:34940175-34940194 MS.gene071026:CDS 40.0%
!! AGTTGATGATGAGGACACAT+TGG - chr7.3:34940299-34940318 None:intergenic 40.0%
!! GGTTGATTGAGTTGATGATG+AGG - chr7.3:34940308-34940327 None:intergenic 40.0%
!! GTTGATGATGAGGACACATT+GGG - chr7.3:34940298-34940317 None:intergenic 40.0%
!!! ATGACTAATTTTTGGGGTGG+AGG + chr7.3:34940124-34940143 MS.gene071026:CDS 40.0%
AACTCTACCACTTTCTGCTC+AGG - chr7.3:34939758-34939777 None:intergenic 45.0%
AATGTCTTTCCTGCTTGGCT+CGG + chr7.3:34939995-34940014 MS.gene071026:CDS 45.0%
ACCACTTTCTGCTCAGGTAA+AGG - chr7.3:34939752-34939771 None:intergenic 45.0%
ACCTTTACCTGAGCAGAAAG+TGG + chr7.3:34939748-34939767 MS.gene071026:CDS 45.0%
ACTTCCAAACCCTTGGAGAA+CGG + chr7.3:34939689-34939708 MS.gene071026:CDS 45.0%
ATCACATACCAGGTTGCAAG+GGG - chr7.3:34940064-34940083 None:intergenic 45.0%
CAGGAAAGACATTGGTCACA+TGG - chr7.3:34939988-34940007 None:intergenic 45.0%
CATCACATACCAGGTTGCAA+GGG - chr7.3:34940065-34940084 None:intergenic 45.0%
CCCCAAGCAACAACACAATT+TGG + chr7.3:34940100-34940119 MS.gene071026:CDS 45.0%
CTGCATTGCCAATTCCACAA+AGG + chr7.3:34940155-34940174 MS.gene071026:CDS 45.0%
GGTTGTGCAAGTTAGCACTA+CGG - chr7.3:34939955-34939974 None:intergenic 45.0%
TGACCAATGTCTTTCCTGCT+TGG + chr7.3:34939990-34940009 MS.gene071026:CDS 45.0%
TGGCATTTGCATCACATACC+AGG - chr7.3:34940074-34940093 None:intergenic 45.0%
TTCTCCACTTCTCAAGCTCT+TGG + chr7.3:34939803-34939822 MS.gene071026:CDS 45.0%
! CCCAAAGTGTTTGACCTTTG+TGG - chr7.3:34940172-34940191 None:intergenic 45.0%
! GCTAACTTGCACAACCCAAT+TGG + chr7.3:34939959-34939978 MS.gene071026:CDS 45.0%
! GTTGTTGCTTGGGGTGAATT+TGG - chr7.3:34940094-34940113 None:intergenic 45.0%
!! GGGATGACTAATTTTTGGGG+TGG + chr7.3:34940121-34940140 MS.gene071026:CDS 45.0%
ACCATATGGTGGCTATGGAG+GGG + chr7.3:34940021-34940040 MS.gene071026:CDS 50.0%
ACCTCCAAGAGCTTGAGAAG+TGG - chr7.3:34939810-34939829 None:intergenic 50.0%
CAAATACACCCCTTGCAACC+TGG + chr7.3:34940053-34940072 MS.gene071026:CDS 50.0%
CAAGTCCACTTCCAAACCCT+TGG + chr7.3:34939682-34939701 MS.gene071026:CDS 50.0%
CACCATATGGTGGCTATGGA+GGG + chr7.3:34940020-34940039 MS.gene071026:CDS 50.0%
CCTCAAACGCAAAATGGTGG+AGG + chr7.3:34939556-34939575 MS.gene071026:CDS 50.0%
GAATGTCCACCGTTCTCCAA+GGG - chr7.3:34939701-34939720 None:intergenic 50.0%
GAGCCAAGCAGGAAAGACAT+TGG - chr7.3:34939996-34940015 None:intergenic 50.0%
GCATCACATACCAGGTTGCA+AGG - chr7.3:34940066-34940085 None:intergenic 50.0%
GGTGTGAGTGCCAAGTCTTA+GGG - chr7.3:34939663-34939682 None:intergenic 50.0%
GGTTCACCATATGGTGGCTA+TGG + chr7.3:34940016-34940035 MS.gene071026:CDS 50.0%
GTTCTCCAAGGGTTTGGAAG+TGG - chr7.3:34939690-34939709 None:intergenic 50.0%
TCACCATATGGTGGCTATGG+AGG + chr7.3:34940019-34940038 MS.gene071026:CDS 50.0%
TCCACTTCTCAAGCTCTTGG+AGG + chr7.3:34939806-34939825 MS.gene071026:CDS 50.0%
TGAATGTCCACCGTTCTCCA+AGG - chr7.3:34939702-34939721 None:intergenic 50.0%
TGGTGTGAGTGCCAAGTCTT+AGG - chr7.3:34939664-34939683 None:intergenic 50.0%
! CAAGGGTTTGGAAGTGGACT+TGG - chr7.3:34939684-34939703 None:intergenic 50.0%
! CAGGTTGCAAGGGGTGTATT+TGG - chr7.3:34940055-34940074 None:intergenic 50.0%
! CCTCCACCATTTTGCGTTTG+AGG - chr7.3:34939559-34939578 None:intergenic 50.0%
ACCCCTCCATAGCCACCATA+TGG - chr7.3:34940025-34940044 None:intergenic 55.0%
TATGGTGAACCGAGCCAAGC+AGG - chr7.3:34940007-34940026 None:intergenic 55.0%
TCCAAACCCTTGGAGAACGG+TGG + chr7.3:34939692-34939711 MS.gene071026:CDS 55.0%
TCCACCGTTCTCCAAGGGTT+TGG - chr7.3:34939696-34939715 None:intergenic 55.0%
TGGTCACATGGGCGCCAATT+GGG - chr7.3:34939976-34939995 None:intergenic 55.0%
TTGGTCACATGGGCGCCAAT+TGG - chr7.3:34939977-34939996 None:intergenic 55.0%
!! GCTTGGCTCGGTTCACCATA+TGG + chr7.3:34940007-34940026 MS.gene071026:CDS 55.0%
!! TGGCTCGGTTCACCATATGG+TGG + chr7.3:34940010-34940029 MS.gene071026:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 34939515 34940330 34939515 ID=MS.gene071026
chr7.3 mRNA 34939515 34940330 34939515 ID=MS.gene071026.t1;Parent=MS.gene071026
chr7.3 exon 34939515 34940330 34939515 ID=MS.gene071026.t1.exon1;Parent=MS.gene071026.t1
chr7.3 CDS 34939515 34940330 34939515 ID=cds.MS.gene071026.t1;Parent=MS.gene071026.t1
Gene Sequence

>MS.gene071026

ATGTCCGAGAGAATTTCCATTGGAAATGATGAGGATAGAAACCTCAAACGCAAAATGGTGGAGGAGCCAAAATTAGAGTCGAAATCAAAGAAGGAGTCAAAGACAATGACACCAACCTCTGAATCTGATGTTGAGGATTTACTAACCCTAAGACTTGGCACTCACACCAAGTCCACTTCCAAACCCTTGGAGAACGGTGGACATTCAAGTTCATTTCAAAACACTACTGATGAACCTTTACCTGAGCAGAAAGTGGTAGAGTTCTCTTGTAATTATTGTGACAAGAAATTCTCCACTTCTCAAGCTCTTGGAGGTCACCAAAATGCTCATAAGCGTGAACGTGTCTTAAAAAAAATGGAAGATAAAAGGAGAGAGGAAGAGATGGATTCAACCCTATGGTTTTCAAGCTCTATTCACTATCAAGGCCATTCATATTTCCGTAGTGCTAACTTGCACAACCCAATTGGCGCCCATGTGACCAATGTCTTTCCTGCTTGGCTCGGTTCACCATATGGTGGCTATGGAGGGGTGTATATGCCAAATACACCCCTTGCAACCTGGTATGTGATGCAAATGCCAAATTCACCCCAAGCAACAACACAATTTGGGATGACTAATTTTTGGGGTGGAGGTCAAAATGCTGCATTGCCAATTCCACAAAGGTCAAACACTTTGGGTTTTGGACTTTTTCCTCAAAGAAATTTCATTGATCAACTTCGTTCTCACACTCTTCCATTATTATCTCGTGATCCTAATAGAGAAGGCATAATTCAGGAAAATCCCAATGTGTCCTCATCATCAACTCAATCAACCTAG

Protein sequence

>MS.gene071026.t1

MSERISIGNDEDRNLKRKMVEEPKLESKSKKESKTMTPTSESDVEDLLTLRLGTHTKSTSKPLENGGHSSSFQNTTDEPLPEQKVVEFSCNYCDKKFSTSQALGGHQNAHKRERVLKKMEDKRREEEMDSTLWFSSSIHYQGHSYFRSANLHNPIGAHVTNVFPAWLGSPYGGYGGVYMPNTPLATWYVMQMPNSPQATTQFGMTNFWGGGQNAALPIPQRSNTLGFGLFPQRNFIDQLRSHTLPLLSRDPNREGIIQENPNVSSSSTQST