Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071077.t1 | XP_024640580.1 | 100 | 172 | 0 | 0 | 1 | 172 | 1 | 172 | 9.80E-77 | 296.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071077.t1 | Q76EJ0 | 55.7 | 167 | 66 | 6 | 9 | 172 | 1 | 162 | 3.7e-40 | 166.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071077.t1 | G7JWX9 | 100.0 | 171 | 0 | 0 | 1 | 171 | 1 | 171 | 2.7e-76 | 294.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene071077.t1 | TF | C2C2-YABBY |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071077.t1 | MTR_5g046230 | 100.000 | 171 | 0 | 0 | 1 | 171 | 1 | 171 | 5.26e-126 | 353 |
| MS.gene071077.t1 | MTR_4g114730 | 43.636 | 165 | 64 | 4 | 8 | 155 | 5 | 157 | 9.68e-41 | 136 |
| MS.gene071077.t1 | MTR_2g087740 | 48.485 | 165 | 67 | 6 | 7 | 155 | 1 | 163 | 8.52e-40 | 134 |
| MS.gene071077.t1 | MTR_4g050300 | 43.312 | 157 | 77 | 3 | 7 | 155 | 1 | 153 | 8.79e-39 | 130 |
| MS.gene071077.t1 | MTR_4g025900 | 44.654 | 159 | 61 | 4 | 19 | 155 | 25 | 178 | 1.53e-35 | 123 |
| MS.gene071077.t1 | MTR_4g101660 | 45.399 | 163 | 71 | 3 | 10 | 155 | 12 | 173 | 4.36e-35 | 122 |
| MS.gene071077.t1 | MTR_5g034030 | 42.568 | 148 | 74 | 2 | 17 | 153 | 12 | 159 | 1.43e-32 | 115 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071077.t1 | AT1G69180 | 66.857 | 175 | 51 | 4 | 1 | 171 | 1 | 172 | 2.86e-64 | 195 |
| MS.gene071077.t1 | AT1G08465 | 48.408 | 157 | 62 | 5 | 12 | 155 | 3 | 153 | 5.55e-40 | 134 |
| MS.gene071077.t1 | AT2G26580 | 44.444 | 153 | 60 | 3 | 15 | 155 | 7 | 146 | 1.71e-38 | 129 |
| MS.gene071077.t1 | AT2G26580 | 44.444 | 153 | 60 | 3 | 15 | 155 | 7 | 146 | 1.71e-38 | 129 |
| MS.gene071077.t1 | AT2G45190 | 47.024 | 168 | 65 | 7 | 10 | 155 | 17 | 182 | 2.59e-37 | 128 |
| MS.gene071077.t1 | AT4G00180 | 39.779 | 181 | 71 | 5 | 10 | 155 | 17 | 194 | 2.51e-31 | 113 |
| MS.gene071077.t1 | AT1G23420 | 37.107 | 159 | 81 | 3 | 18 | 157 | 20 | 178 | 4.23e-28 | 104 |
| MS.gene071077.t1 | AT1G23420 | 37.342 | 158 | 80 | 3 | 19 | 157 | 52 | 209 | 7.97e-28 | 105 |
| MS.gene071077.t1 | AT4G00180 | 35.758 | 165 | 69 | 4 | 26 | 155 | 1 | 163 | 6.34e-22 | 88.2 |
| MS.gene071077.t1 | AT2G45190 | 72.727 | 55 | 14 | 1 | 101 | 155 | 65 | 118 | 3.94e-21 | 85.1 |
Find 37 sgRNAs with CRISPR-Local
Find 85 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATTGAACTGGTTGGTGAATT+TGG | 0.207087 | 5.1:-34297450 | None:intergenic |
| CCATGGTAACTTTCTCTTCA+TGG | 0.253602 | 5.1:-34295967 | None:intergenic |
| ACAACAAATGGCGGTGGTCT+AGG | 0.360978 | 5.1:-34296750 | None:intergenic |
| TGCTTACGTTTGACAACAAA+TGG | 0.370125 | 5.1:-34296762 | None:intergenic |
| CAACAAATGGCGGTGGTCTA+GGG | 0.442200 | 5.1:-34296749 | None:intergenic |
| TTTAGTTGCAGAAATTGAAC+TGG | 0.447192 | 5.1:-34297463 | None:intergenic |
| GGAAGACGGTGCTTCTTCTC+AGG | 0.456267 | 5.1:-34296943 | None:intergenic |
| ATTGCACTTACTTCATGAAA+CGG | 0.472131 | 5.1:-34296978 | None:intergenic |
| TGATCACACCCTCAGTCTCC+AGG | 0.486490 | 5.1:+34296441 | MS.gene071077:CDS |
| CAACTTTATCACAGTGGGCT+AGG | 0.506444 | 5.1:+34297421 | MS.gene071077:intron |
| AGAGAAAGTTACCATGGACT+TGG | 0.516644 | 5.1:+34295973 | MS.gene071077:CDS |
| CACTGAAAGCTTCTCGATGT+GGG | 0.523718 | 5.1:-34297250 | None:intergenic |
| TTTGGAACTTGGTGGTCTTG+TGG | 0.524145 | 5.1:-34296407 | None:intergenic |
| GCTTCTCGATGTGGGATCTG+AGG | 0.524810 | 5.1:-34297242 | None:intergenic |
| CTTCTCGATGTGGGATCTGA+GGG | 0.529903 | 5.1:-34297241 | None:intergenic |
| ATGAAACGGTTGTATGCAGA+TGG | 0.547267 | 5.1:-34296964 | None:intergenic |
| GCACTGAAAGCTTCTCGATG+TGG | 0.561704 | 5.1:-34297251 | None:intergenic |
| TCGATGTGGGATCTGAGGGT+TGG | 0.564009 | 5.1:-34297237 | None:intergenic |
| ATTATTGAACCTGGAGACTG+AGG | 0.580682 | 5.1:-34296450 | None:intergenic |
| TAACAAAGGTGTTGATCAGA+TGG | 0.588546 | 5.1:-34296001 | None:intergenic |
| AGTGACTCAGTTGATGTAGT+TGG | 0.590905 | 5.1:-34296723 | None:intergenic |
| GTTGCAGAAATTGAACTGGT+TGG | 0.594902 | 5.1:-34297459 | None:intergenic |
| AACAAATGGCGGTGGTCTAG+GGG | 0.595027 | 5.1:-34296748 | None:intergenic |
| GTTGGTGAAGAAGAAGATGA+TGG | 0.609575 | 5.1:-34296705 | None:intergenic |
| CGTTTGACAACAAATGGCGG+TGG | 0.615312 | 5.1:-34296756 | None:intergenic |
| AGATGGTGGAACCAAGTCCA+TGG | 0.626957 | 5.1:-34295984 | None:intergenic |
| CCATGAAGAGAAAGTTACCA+TGG | 0.629385 | 5.1:+34295967 | MS.gene071077:CDS |
| GTATCTAGCAGCCTCTTGCA+TGG | 0.634316 | 5.1:-34296340 | None:intergenic |
| CAAAGGTGTTGATCAGATGG+TGG | 0.641754 | 5.1:-34295998 | None:intergenic |
| GGTTGTATGCAGATGGAAGA+CGG | 0.643999 | 5.1:-34296957 | None:intergenic |
| GTGAAGAAGAAGATGATGGT+TGG | 0.655028 | 5.1:-34296701 | None:intergenic |
| AGTTGCAACGAACGTAACAA+AGG | 0.660534 | 5.1:-34296015 | None:intergenic |
| AGGTTGGCATACCATGCAAG+AGG | 0.666035 | 5.1:+34296329 | MS.gene071077:intron |
| TTATTGAACCTGGAGACTGA+GGG | 0.671255 | 5.1:-34296449 | None:intergenic |
| TTACGTTTGACAACAAATGG+CGG | 0.678472 | 5.1:-34296759 | None:intergenic |
| GATACTGTGACAGTGAAGTG+TGG | 0.709816 | 5.1:+34296358 | MS.gene071077:CDS |
| GTCTTGTGGTGAGAAAAGAG+AGG | 0.744847 | 5.1:-34296393 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATTAATTAATTAATAAAA+AGG | - | chr5.1:34296617-34296636 | None:intergenic | 0.0% |
| !! | TGATATATGAATAATATTAA+AGG | - | chr5.1:34296075-34296094 | None:intergenic | 10.0% |
| !!! | AAATTTTTCTAAAATATGTA+GGG | + | chr5.1:34296285-34296304 | MS.gene071077:intron | 10.0% |
| !!! | AGTATATATTATTTTAAACA+GGG | - | chr5.1:34297118-34297137 | None:intergenic | 10.0% |
| !!! | TAAATTTTTCTAAAATATGT+AGG | + | chr5.1:34296284-34296303 | MS.gene071077:intron | 10.0% |
| !!! | TAGTATATATTATTTTAAAC+AGG | - | chr5.1:34297119-34297138 | None:intergenic | 10.0% |
| !!! | TTTTTTATTAGCTACATATA+TGG | + | chr5.1:34296238-34296257 | MS.gene071077:intron | 15.0% |
| !! | TTTAATACTGTCTCTAAATT+TGG | + | chr5.1:34296200-34296219 | MS.gene071077:intron | 20.0% |
| !!! | ATATTATTTTAAACAGGGTA+AGG | - | chr5.1:34297113-34297132 | None:intergenic | 20.0% |
| !!! | ATGAAAAATGCAAACATTTT+TGG | - | chr5.1:34297282-34297301 | None:intergenic | 20.0% |
| !!! | ATGATGAGTTTTATATGTAT+TGG | + | chr5.1:34296590-34296609 | MS.gene071077:intron | 20.0% |
| !!! | CATCTATATTTTTTGTTTTG+AGG | + | chr5.1:34296512-34296531 | MS.gene071077:intron | 20.0% |
| !!! | CCTCTTTTATTTTCTTATTA+GGG | + | chr5.1:34297302-34297321 | MS.gene071077:intron | 20.0% |
| !!! | TATTATTTTAAACAGGGTAA+GGG | - | chr5.1:34297112-34297131 | None:intergenic | 20.0% |
| !!! | TCCTCTTTTATTTTCTTATT+AGG | + | chr5.1:34297301-34297320 | MS.gene071077:intron | 20.0% |
| ! | CCCTAATAAGAAAATAAAAG+AGG | - | chr5.1:34297305-34297324 | None:intergenic | 25.0% |
| ! | CTAAACAAAATTATTGAACC+TGG | - | chr5.1:34296462-34296481 | None:intergenic | 25.0% |
| ! | TAATATATATAGCACTGCTT+TGG | + | chr5.1:34297334-34297353 | MS.gene071077:intron | 25.0% |
| ATTCCAAGACTATAGTATTC+TGG | + | chr5.1:34297534-34297553 | MS.gene071077:intron | 30.0% | |
| ATTGCACTTACTTCATGAAA+CGG | - | chr5.1:34296981-34297000 | None:intergenic | 30.0% | |
| CTTCGTCTATAATTTCGTAT+AGG | - | chr5.1:34296173-34296192 | None:intergenic | 30.0% | |
| GATATTTCCTTAGAGAGTAA+TGG | - | chr5.1:34296798-34296817 | None:intergenic | 30.0% | |
| TAACCAGAATACTATAGTCT+TGG | - | chr5.1:34297540-34297559 | None:intergenic | 30.0% | |
| TTACACAACTTTATCACAGT+GGG | + | chr5.1:34297416-34297435 | MS.gene071077:intron | 30.0% | |
| TTAGAGAGTAATGGTGAAAA+GGG | - | chr5.1:34296789-34296808 | None:intergenic | 30.0% | |
| TTGTGTAACACAAGTTAGTT+TGG | - | chr5.1:34297404-34297423 | None:intergenic | 30.0% | |
| TTTAGTTGCAGAAATTGAAC+TGG | - | chr5.1:34297466-34297485 | None:intergenic | 30.0% | |
| ! | AGTTAACAAGTTGAGAATGA+TGG | - | chr5.1:34296103-34296122 | None:intergenic | 30.0% |
| ! | TGTGTAACACAAGTTAGTTT+GGG | - | chr5.1:34297403-34297422 | None:intergenic | 30.0% |
| !! | AATACTTTTGTTTGTAGCAG+GGG | + | chr5.1:34296658-34296677 | MS.gene071077:intron | 30.0% |
| !! | AGAATACTTTTGTTTGTAGC+AGG | + | chr5.1:34296656-34296675 | MS.gene071077:intron | 30.0% |
| !! | ATACTTTTTATCTGTCAGAG+AGG | + | chr5.1:34297193-34297212 | MS.gene071077:intron | 30.0% |
| !! | GAATACTTTTGTTTGTAGCA+GGG | + | chr5.1:34296657-34296676 | MS.gene071077:intron | 30.0% |
| !! | GTGATCAACAGTTTGATTTT+TGG | - | chr5.1:34296428-34296447 | None:intergenic | 30.0% |
| !! | GTTTGTTATGTTATGTAGGT+TGG | + | chr5.1:34296313-34296332 | MS.gene071077:intron | 30.0% |
| !! | TGTGGTTTGTTATGTTATGT+AGG | + | chr5.1:34296309-34296328 | MS.gene071077:intron | 30.0% |
| !! | TTCTAAAATATGTAGGGTTG+TGG | + | chr5.1:34296291-34296310 | MS.gene071077:intron | 30.0% |
| !!! | ACAGTTTGATTTTTGGAACT+TGG | - | chr5.1:34296421-34296440 | None:intergenic | 30.0% |
| AACCAACCATAACTACAAGA+TGG | + | chr5.1:34297071-34297090 | MS.gene071077:intron | 35.0% | |
| CTTAGAGAGTAATGGTGAAA+AGG | - | chr5.1:34296790-34296809 | None:intergenic | 35.0% | |
| GGGATTTACAGTAGCAAAAA+AGG | + | chr5.1:34296678-34296697 | MS.gene071077:CDS | 35.0% | |
| GTGTTACCATCTTGTAGTTA+TGG | - | chr5.1:34297080-34297099 | None:intergenic | 35.0% | |
| GTTACACAACTTTATCACAG+TGG | + | chr5.1:34297415-34297434 | MS.gene071077:intron | 35.0% | |
| TACCATCTTGTAGTTATGGT+TGG | - | chr5.1:34297076-34297095 | None:intergenic | 35.0% | |
| TGCTTACGTTTGACAACAAA+TGG | - | chr5.1:34296765-34296784 | None:intergenic | 35.0% | |
| TTACGTTTGACAACAAATGG+CGG | - | chr5.1:34296762-34296781 | None:intergenic | 35.0% | |
| ! | AACAAGTTGAGAATGATGGA+TGG | - | chr5.1:34296099-34296118 | None:intergenic | 35.0% |
| ! | CTATAGTCTTGGAATATGCA+TGG | - | chr5.1:34297529-34297548 | None:intergenic | 35.0% |
| ! | CTTTTCACCATTACTCTCTA+AGG | + | chr5.1:34296788-34296807 | MS.gene071077:intron | 35.0% |
| ! | TTTTGTATGCTTATGTGTGC+AGG | + | chr5.1:34297565-34297584 | MS.gene071077:intron | 35.0% |
| !! | ATTGAACTGGTTGGTGAATT+TGG | - | chr5.1:34297453-34297472 | None:intergenic | 35.0% |
| !! | TAACAAAGGTGTTGATCAGA+TGG | - | chr5.1:34296004-34296023 | None:intergenic | 35.0% |
| !!! | GTTTGATTTTTGGAACTTGG+TGG | - | chr5.1:34296418-34296437 | None:intergenic | 35.0% |
| AGAGAAAGTTACCATGGACT+TGG | + | chr5.1:34295973-34295992 | MS.gene071077:CDS | 40.0% | |
| AGTTGCAACGAACGTAACAA+AGG | - | chr5.1:34296018-34296037 | None:intergenic | 40.0% | |
| ATGAAACGGTTGTATGCAGA+TGG | - | chr5.1:34296967-34296986 | None:intergenic | 40.0% | |
| ATTATTGAACCTGGAGACTG+AGG | - | chr5.1:34296453-34296472 | None:intergenic | 40.0% | |
| CCATGAAGAGAAAGTTACCA+TGG | + | chr5.1:34295967-34295986 | MS.gene071077:CDS | 40.0% | |
| CCATGGTAACTTTCTCTTCA+TGG | - | chr5.1:34295970-34295989 | None:intergenic | 40.0% | |
| GGTGAAGTTTACATACAGCT+AGG | - | chr5.1:34296054-34296073 | None:intergenic | 40.0% | |
| GTTGCAGAAATTGAACTGGT+TGG | - | chr5.1:34297462-34297481 | None:intergenic | 40.0% | |
| TTATTGAACCTGGAGACTGA+GGG | - | chr5.1:34296452-34296471 | None:intergenic | 40.0% | |
| ! | AGTGACTCAGTTGATGTAGT+TGG | - | chr5.1:34296726-34296745 | None:intergenic | 40.0% |
| ! | GTGAAGAAGAAGATGATGGT+TGG | - | chr5.1:34296704-34296723 | None:intergenic | 40.0% |
| ! | GTTGGTGAAGAAGAAGATGA+TGG | - | chr5.1:34296708-34296727 | None:intergenic | 40.0% |
| CACTGAAAGCTTCTCGATGT+GGG | - | chr5.1:34297253-34297272 | None:intergenic | 45.0% | |
| GATACTGTGACAGTGAAGTG+TGG | + | chr5.1:34296358-34296377 | MS.gene071077:CDS | 45.0% | |
| GGTTGTATGCAGATGGAAGA+CGG | - | chr5.1:34296960-34296979 | None:intergenic | 45.0% | |
| GTCTTGTGGTGAGAAAAGAG+AGG | - | chr5.1:34296396-34296415 | None:intergenic | 45.0% | |
| ! | CAACTTTATCACAGTGGGCT+AGG | + | chr5.1:34297421-34297440 | MS.gene071077:intron | 45.0% |
| ! | TTTGGAACTTGGTGGTCTTG+TGG | - | chr5.1:34296410-34296429 | None:intergenic | 45.0% |
| !! | CAAAGGTGTTGATCAGATGG+TGG | - | chr5.1:34296001-34296020 | None:intergenic | 45.0% |
| AACAAATGGCGGTGGTCTAG+GGG | - | chr5.1:34296751-34296770 | None:intergenic | 50.0% | |
| ACAACAAATGGCGGTGGTCT+AGG | - | chr5.1:34296753-34296772 | None:intergenic | 50.0% | |
| AGGTTGGCATACCATGCAAG+AGG | + | chr5.1:34296329-34296348 | MS.gene071077:intron | 50.0% | |
| CAACAAATGGCGGTGGTCTA+GGG | - | chr5.1:34296752-34296771 | None:intergenic | 50.0% | |
| CGTTTGACAACAAATGGCGG+TGG | - | chr5.1:34296759-34296778 | None:intergenic | 50.0% | |
| CTTCTCGATGTGGGATCTGA+GGG | - | chr5.1:34297244-34297263 | None:intergenic | 50.0% | |
| GCACTGAAAGCTTCTCGATG+TGG | - | chr5.1:34297254-34297273 | None:intergenic | 50.0% | |
| GTATCTAGCAGCCTCTTGCA+TGG | - | chr5.1:34296343-34296362 | None:intergenic | 50.0% | |
| !! | AGATGGTGGAACCAAGTCCA+TGG | - | chr5.1:34295987-34296006 | None:intergenic | 50.0% |
| GCTTCTCGATGTGGGATCTG+AGG | - | chr5.1:34297245-34297264 | None:intergenic | 55.0% | |
| GGAAGACGGTGCTTCTTCTC+AGG | - | chr5.1:34296946-34296965 | None:intergenic | 55.0% | |
| TCGATGTGGGATCTGAGGGT+TGG | - | chr5.1:34297240-34297259 | None:intergenic | 55.0% | |
| TGATCACACCCTCAGTCTCC+AGG | + | chr5.1:34296441-34296460 | MS.gene071077:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 34295962 | 34297592 | 34295962 | ID=MS.gene071077 |
| chr5.1 | mRNA | 34295962 | 34297592 | 34295962 | ID=MS.gene071077.t1;Parent=MS.gene071077 |
| chr5.1 | exon | 34295962 | 34296057 | 34295962 | ID=MS.gene071077.t1.exon1;Parent=MS.gene071077.t1 |
| chr5.1 | CDS | 34295962 | 34296057 | 34295962 | ID=cds.MS.gene071077.t1;Parent=MS.gene071077.t1 |
| chr5.1 | exon | 34296331 | 34296462 | 34296331 | ID=MS.gene071077.t1.exon2;Parent=MS.gene071077.t1 |
| chr5.1 | CDS | 34296331 | 34296462 | 34296331 | ID=cds.MS.gene071077.t1;Parent=MS.gene071077.t1 |
| chr5.1 | exon | 34296678 | 34296777 | 34296678 | ID=MS.gene071077.t1.exon3;Parent=MS.gene071077.t1 |
| chr5.1 | CDS | 34296678 | 34296777 | 34296678 | ID=cds.MS.gene071077.t1;Parent=MS.gene071077.t1 |
| chr5.1 | exon | 34296941 | 34296989 | 34296941 | ID=MS.gene071077.t1.exon4;Parent=MS.gene071077.t1 |
| chr5.1 | CDS | 34296941 | 34296989 | 34296941 | ID=cds.MS.gene071077.t1;Parent=MS.gene071077.t1 |
| chr5.1 | exon | 34297211 | 34297286 | 34297211 | ID=MS.gene071077.t1.exon5;Parent=MS.gene071077.t1 |
| chr5.1 | CDS | 34297211 | 34297286 | 34297211 | ID=cds.MS.gene071077.t1;Parent=MS.gene071077.t1 |
| chr5.1 | exon | 34297435 | 34297494 | 34297435 | ID=MS.gene071077.t1.exon6;Parent=MS.gene071077.t1 |
| chr5.1 | CDS | 34297435 | 34297494 | 34297435 | ID=cds.MS.gene071077.t1;Parent=MS.gene071077.t1 |
| chr5.1 | exon | 34297587 | 34297592 | 34297587 | ID=MS.gene071077.t1.exon7;Parent=MS.gene071077.t1 |
| chr5.1 | CDS | 34297587 | 34297592 | 34297587 | ID=cds.MS.gene071077.t1;Parent=MS.gene071077.t1 |
| Gene Sequence |
| Protein sequence |
