AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071190

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071190.t1 MTR_4g064150 93.605 735 36 1 1 724 1 735 0.0 1388
MS.gene071190.t1 MTR_4g064120 71.988 664 136 7 62 724 14 628 0.0 931
MS.gene071190.t1 MTR_4g064180 71.687 664 138 8 62 724 14 628 0.0 918
MS.gene071190.t1 MTR_2g097410 56.516 775 254 17 1 724 1 743 0.0 816
MS.gene071190.t1 MTR_4g064160 49.277 692 281 13 72 724 26 686 0.0 606
MS.gene071190.t1 MTR_7g062120 45.007 691 330 17 72 724 64 742 0.0 544
MS.gene071190.t1 MTR_2g097467 44.461 668 297 18 75 724 45 656 7.39e-175 516
MS.gene071190.t1 MTR_4g064200 52.446 511 219 9 228 724 152 652 4.91e-174 513
MS.gene071190.t1 MTR_2g097473 44.444 666 300 17 75 724 45 656 1.93e-171 507
MS.gene071190.t1 MTR_2g097463 43.297 649 312 13 85 724 56 657 8.98e-167 495
MS.gene071190.t1 MTR_2g097310 48.117 478 231 7 249 724 178 640 6.22e-157 469
MS.gene071190.t1 MTR_2g097350 50.342 439 202 7 288 724 218 642 4.10e-150 451
MS.gene071190.t1 MTR_2g097390 47.598 458 221 8 272 724 200 643 1.49e-147 445
MS.gene071190.t1 MTR_2g097380 45.295 457 189 9 272 724 164 563 4.86e-131 400
MS.gene071190.t1 MTR_2g082090 32.713 376 248 4 346 721 208 578 6.01e-67 231
MS.gene071190.t1 MTR_5g097480 31.915 376 251 3 346 721 173 543 2.90e-62 218
MS.gene071190.t1 MTR_3g056110 32.540 378 246 6 346 721 171 541 9.34e-60 211
MS.gene071190.t1 MTR_2g026250 29.630 378 260 4 345 721 223 595 6.00e-58 207
MS.gene071190.t1 MTR_4g133660 30.570 386 248 8 345 722 170 543 1.94e-56 202
MS.gene071190.t1 MTR_5g094450 31.606 386 243 9 346 721 157 531 1.01e-55 199
MS.gene071190.t1 MTR_8g442410 30.250 400 243 7 342 724 147 527 3.86e-53 192
MS.gene071190.t1 MTR_1g029420 28.610 374 262 3 348 721 223 591 1.38e-52 192
MS.gene071190.t1 MTR_7g027190 31.034 406 233 8 340 721 290 672 1.67e-52 193
MS.gene071190.t1 MTR_3g089055 28.610 374 262 3 348 721 201 569 1.89e-52 191
MS.gene071190.t1 MTR_3g089055 28.610 374 262 3 348 721 196 564 1.99e-52 191
MS.gene071190.t1 MTR_4g104020 29.648 398 249 8 339 721 138 519 3.64e-52 189
MS.gene071190.t1 MTR_3g065980 30.193 414 252 8 325 721 146 539 4.26e-49 181
MS.gene071190.t1 MTR_7g074650 30.000 370 241 7 354 723 442 793 1.35e-45 175
MS.gene071190.t1 MTR_4g076140 27.074 458 262 9 326 721 21 468 2.33e-44 166
MS.gene071190.t1 MTR_2g034260 29.648 398 250 10 340 721 200 583 3.10e-43 165
MS.gene071190.t1 MTR_2g034250 29.620 395 255 9 340 721 200 584 4.26e-42 162
MS.gene071190.t1 MTR_5g009080 27.126 435 247 10 347 721 53 477 4.52e-40 154
MS.gene071190.t1 MTR_3g022830 26.128 421 250 8 351 721 26 435 9.50e-40 152
MS.gene071190.t1 MTR_4g102790 26.546 388 263 10 346 723 288 663 1.68e-38 152
MS.gene071190.t1 MTR_4g076020 26.954 371 244 5 354 724 88 431 4.07e-38 147
MS.gene071190.t1 MTR_4g097080 28.052 385 257 10 348 723 131 504 6.44e-37 145
MS.gene071190.t1 MTR_2g089100 26.406 409 258 9 347 724 49 445 1.02e-36 144
MS.gene071190.t1 MTR_1g069725 27.763 389 245 14 342 721 101 462 4.65e-36 142
MS.gene071190.t1 MTR_2g034280 27.735 393 262 9 340 721 193 574 3.24e-35 141
MS.gene071190.t1 MTR_1g096030 30.000 400 237 14 347 721 77 458 1.27e-33 135
MS.gene071190.t1 MTR_3g072710 26.551 403 264 10 339 721 109 499 2.04e-32 132
MS.gene071190.t1 MTR_5g015490 25.654 382 274 5 349 723 113 491 1.66e-31 129
MS.gene071190.t1 MTR_4g077760 26.923 364 240 9 366 721 209 554 2.78e-31 129
MS.gene071190.t1 MTR_7g109580 28.205 390 257 12 340 721 179 553 1.53e-30 127
MS.gene071190.t1 MTR_5g015950 23.709 426 268 11 347 724 32 448 1.58e-30 125
MS.gene071190.t1 MTR_3g053270 23.709 426 268 11 347 724 32 448 1.58e-30 125
MS.gene071190.t1 MTR_4g095500 24.582 419 271 9 347 724 56 470 1.84e-30 125
MS.gene071190.t1 MTR_5g058860 28.463 397 241 14 351 722 119 497 8.78e-30 124
MS.gene071190.t1 MTR_7g057230 24.793 363 252 7 366 722 310 657 2.20e-29 124
MS.gene071190.t1 MTR_6g047750 26.031 388 254 12 348 721 293 661 1.07e-28 122
MS.gene071190.t1 MTR_1g086970 26.185 401 258 13 340 721 96 477 5.96e-28 118
MS.gene071190.t1 MTR_8g093070 26.650 409 247 17 335 724 124 498 3.19e-27 116
MS.gene071190.t1 MTR_2g099110 25.667 300 213 4 350 648 74 364 9.45e-22 99.4
MS.gene071190.t1 MTR_0092s0100 22.222 396 287 7 327 721 355 730 1.86e-14 77.8
MS.gene071190.t1 MTR_0092s0100 22.222 396 287 7 327 721 352 727 2.32e-14 77.4
MS.gene071190.t1 MTR_4g026485 27.536 276 180 7 351 622 270 529 2.35e-14 77.0
MS.gene071190.t1 MTR_8g020840 24.093 386 234 14 350 708 171 524 1.12e-13 74.7
MS.gene071190.t1 MTR_3g087300 44.000 75 32 2 305 369 134 208 6.59e-11 62.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071190.t1 AT1G07530 47.143 700 290 17 85 724 88 767 0.0 577
MS.gene071190.t1 AT2G29060 47.641 657 281 15 77 723 53 656 0.0 560
MS.gene071190.t1 AT2G29060 47.455 668 303 14 77 723 53 693 0.0 556
MS.gene071190.t1 AT2G29065 45.622 651 284 15 85 724 39 630 0.0 531
MS.gene071190.t1 AT1G07520 46.142 648 318 11 85 724 71 695 2.93e-179 528
MS.gene071190.t1 AT1G07520 46.142 648 318 11 85 724 72 696 2.99e-179 528
MS.gene071190.t1 AT1G07520 50.947 528 241 8 203 724 16 531 5.63e-178 519
MS.gene071190.t1 AT1G07520 50.947 528 241 8 203 724 16 531 5.63e-178 519
MS.gene071190.t1 AT2G37650 43.491 676 327 18 72 723 58 702 1.08e-166 496
MS.gene071190.t1 AT2G37650 43.491 676 327 18 72 723 70 714 1.10e-166 497
MS.gene071190.t1 AT3G46600 49.889 451 209 5 274 724 116 549 2.12e-151 452
MS.gene071190.t1 AT3G46600 49.889 451 209 5 274 724 148 581 2.80e-151 452
MS.gene071190.t1 AT5G59450 47.500 480 233 7 253 724 132 600 7.37e-148 445
MS.gene071190.t1 AT3G46600 52.646 378 174 2 347 724 1 373 1.73e-140 417
MS.gene071190.t1 AT2G04890 31.915 376 244 4 346 721 49 412 1.90e-59 206
MS.gene071190.t1 AT1G50600 31.915 376 251 3 346 721 155 525 5.01e-59 208
MS.gene071190.t1 AT1G50600 31.915 376 251 3 346 721 226 596 1.95e-58 209
MS.gene071190.t1 AT1G50600 31.915 376 251 3 346 721 230 600 2.12e-58 209
MS.gene071190.t1 AT5G48150 30.319 376 258 3 346 721 118 489 1.53e-56 201
MS.gene071190.t1 AT5G48150 30.319 376 258 3 346 721 118 489 1.53e-56 201
MS.gene071190.t1 AT5G48150 30.319 376 258 3 346 721 118 489 1.53e-56 201
MS.gene071190.t1 AT5G48150 30.319 376 258 3 346 721 118 489 1.53e-56 201
MS.gene071190.t1 AT1G21450 30.319 376 254 5 348 721 223 592 4.46e-54 196
MS.gene071190.t1 AT4G17230 30.676 414 277 5 314 723 119 526 3.68e-52 189
MS.gene071190.t1 AT4G17230 30.676 414 277 5 314 723 119 526 3.68e-52 189
MS.gene071190.t1 AT1G66350 31.234 381 233 10 345 721 150 505 2.51e-47 176
MS.gene071190.t1 AT1G50420 25.463 432 258 5 347 721 54 478 5.31e-46 171
MS.gene071190.t1 AT3G54220 30.025 393 258 8 332 723 275 651 7.45e-46 174
MS.gene071190.t1 AT2G01570 29.948 384 240 10 345 721 219 580 9.50e-46 172
MS.gene071190.t1 AT3G03450 28.460 383 252 9 345 721 178 544 2.75e-45 171
MS.gene071190.t1 AT1G14920 30.105 382 242 9 345 721 167 528 3.21e-45 170
MS.gene071190.t1 AT5G17490 25.952 420 281 10 309 721 119 515 1.41e-41 159
MS.gene071190.t1 AT5G41920 28.084 381 253 6 344 721 37 399 9.50e-41 154
MS.gene071190.t1 AT5G66770 26.667 405 279 8 322 721 192 583 2.13e-36 145
MS.gene071190.t1 AT5G52510 26.154 390 256 10 348 721 266 639 1.11e-35 144
MS.gene071190.t1 AT1G63100 30.931 333 216 8 346 675 273 594 1.49e-35 143
MS.gene071190.t1 AT1G63100 30.931 333 216 8 346 675 248 569 1.55e-35 143
MS.gene071190.t1 AT4G37650 25.191 393 269 10 349 723 145 530 1.37e-33 136
MS.gene071190.t1 AT3G50650 28.972 321 209 5 359 673 184 491 9.23e-33 134
MS.gene071190.t1 AT3G49950 26.854 391 264 10 347 723 27 409 8.53e-31 125
MS.gene071190.t1 AT1G55580 24.821 419 256 11 347 721 41 444 3.79e-25 109
MS.gene071190.t1 AT4G08250 26.448 397 262 14 340 721 95 476 9.67e-25 108
MS.gene071190.t1 AT3G13840 23.023 430 255 16 320 721 128 509 4.35e-14 75.9

Find 161 sgRNAs with CRISPR-Local

Find 182 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGGAATTAATGGACAAATTT+AGG 0.087513 4.2:-40902514 MS.gene071190:CDS
GATGAGATTTAAGAATTTAT+TGG 0.142030 4.2:-40902885 MS.gene071190:CDS
ACCCTCCATATCATTGATTT+TGG 0.161875 4.2:-40903181 MS.gene071190:CDS
TCCATTGAATTCGGAATTAA+TGG 0.174492 4.2:-40902525 MS.gene071190:CDS
AATCAATATGATAGCTTTCT+TGG 0.191887 4.2:-40904423 MS.gene071190:CDS
ATGCCATACCATCGCGTTAT+TGG 0.192133 4.2:-40902970 MS.gene071190:CDS
CATTCTTTGCTACACGGTTT+AGG 0.223208 4.2:-40902754 MS.gene071190:CDS
AAATGCAGAACCGCATTTCT+AGG 0.241890 4.2:+40902839 None:intergenic
CTCCCTAACCATATCCAAAT+TGG 0.268685 4.2:+40903828 None:intergenic
TTTGTTCAGTCAATTGTTAA+TGG 0.288838 4.2:-40902791 MS.gene071190:CDS
ATCAGAGTTATGAATATCTA+AGG 0.292408 4.2:+40904158 None:intergenic
TGAATCATCCAATTTGGATA+TGG 0.301149 4.2:-40903836 MS.gene071190:CDS
CAAACAGCTTCCATTGAATT+CGG 0.304055 4.2:-40902534 MS.gene071190:CDS
ATTCTTTGCTACACGGTTTA+GGG 0.306681 4.2:-40902753 MS.gene071190:CDS
TCACTAGCTTGAGGAAAGTT+TGG 0.320173 4.2:+40904543 None:intergenic
CATCCAATTTGGATATGGTT+AGG 0.330014 4.2:-40903831 MS.gene071190:CDS
TGCCATACCATCGCGTTATT+GGG 0.330847 4.2:-40902969 MS.gene071190:CDS
ACTCTATCCCGGTGATCTTC+AGG 0.333841 4.2:+40903089 None:intergenic
GGATACCAAAATCAATGATA+TGG 0.351602 4.2:+40903176 None:intergenic
CGAGTCTTCCCTCCATTTGA+TGG 0.357746 4.2:+40903571 None:intergenic
AGAAAGCTATCATATTGATT+TGG 0.360772 4.2:+40904426 None:intergenic
AGGAAGCTATCATATTGATT+TGG 0.360772 4.2:+40904459 None:intergenic
TTTATATGCTTCCACATGTT+GGG 0.369926 4.2:-40902408 MS.gene071190:CDS
CATATAAACAGTGGCAGGTT+AGG 0.372839 4.2:-40902574 MS.gene071190:CDS
TGATCAGAAACAGGAGTATC+AGG 0.375983 4.2:+40904066 None:intergenic
ATTGCTGTTGCTGTTGCTGC+TGG 0.378194 4.2:+40904128 None:intergenic
GGCCTTGAGGCACGCCTTGT+CGG 0.379088 4.2:-40903370 MS.gene071190:CDS
CTTCCCTATAGAAGGTTATA+TGG 0.379175 4.2:-40904370 MS.gene071190:CDS
ATTAGCATTCCTGTTGTCAT+TGG 0.384143 4.2:+40903471 None:intergenic
CGCCTTGTCGGCGATGGTAC+TGG 0.387590 4.2:-40903358 MS.gene071190:CDS
TAGTGATGGACTCAGAGATT+TGG 0.396758 4.2:-40903983 MS.gene071190:CDS
ATATCTAAGGGGTGTTGATT+AGG 0.398335 4.2:+40904171 None:intergenic
ATCCCGGTGATCTTCAGGTT+TGG 0.401423 4.2:+40903094 None:intergenic
GGGTGTTGATTAGGAGAAAG+AGG 0.402256 4.2:+40904180 None:intergenic
ATTATCAGAGATGTTTGATA+AGG 0.416031 4.2:-40903677 MS.gene071190:CDS
AGAGAATCCGTTTGTGTTGA+AGG 0.418700 4.2:-40903803 MS.gene071190:CDS
TCCATTAATTCCGAATTCAA+TGG 0.422266 4.2:+40902524 None:intergenic
TACTACCACTTTGGTGAAGA+AGG 0.425321 4.2:-40904507 MS.gene071190:CDS
AACAGGAGTATCAGGTGAAA+GGG 0.430936 4.2:+40904074 None:intergenic
GAGGCCATATAACCTTCTAT+AGG 0.431388 4.2:+40904366 None:intergenic
CTGCCACTGTTTATATGTCT+CGG 0.432575 4.2:+40902580 None:intergenic
ATGAGCTACTGAAGCAGATT+AGG 0.435797 4.2:-40903450 MS.gene071190:CDS
GCCACCACCTTCCCTATAGA+AGG 0.439615 4.2:-40904378 MS.gene071190:CDS
AGGCAACATTCTTCACCCTC+TGG 0.447707 4.2:-40903430 MS.gene071190:CDS
CTGTATATGCCAATGACAAC+AGG 0.450042 4.2:-40903480 MS.gene071190:CDS
ACAAACGGTTACTACCACTT+TGG 0.452669 4.2:-40904516 MS.gene071190:CDS
AGAATGAAACAATAGATTTG+AGG 0.453249 4.2:-40903528 MS.gene071190:CDS
GGCAACATTCTTCACCCTCT+GGG 0.453926 4.2:-40903429 MS.gene071190:CDS
ATGGAAAGAATCGAAGAAAC+TGG 0.455010 4.2:-40903043 MS.gene071190:CDS
ATTTCTCAACCTCATTCTAC+TGG 0.458162 4.2:-40904009 MS.gene071190:CDS
TTTCTTGGTGCTGATCCACT+TGG 0.459039 4.2:-40904408 MS.gene071190:CDS
GTTTCTTCGATTCTTTCCAT+TGG 0.459734 4.2:+40903046 None:intergenic
TTCAAGAAAAGTTTAGAAGA+AGG 0.460061 4.2:-40903901 MS.gene071190:CDS
AGGCCATATAACCTTCTATA+GGG 0.460382 4.2:+40904367 None:intergenic
ATCCAATTTGGATATGGTTA+GGG 0.463279 4.2:-40903830 MS.gene071190:CDS
TTTCTACCCTTCAACACAAA+CGG 0.464697 4.2:+40903796 None:intergenic
CAGTCATTTGTAAGCTAAGT+GGG 0.470257 4.2:+40904241 None:intergenic
GTGGTAACATTGCTGTAACT+TGG 0.472903 4.2:+40902490 None:intergenic
AGGTTATATGGCCTCGGCGA+CGG 0.474767 4.2:-40904358 MS.gene071190:CDS
GACAACAATTGGATGCTTCA+AGG 0.476004 4.2:-40902446 MS.gene071190:CDS
GGGATAGAGTTCCCTTTACC+TGG 0.477006 4.2:-40903076 MS.gene071190:CDS
GTAGAGATCCTCTTGGTGTT+CGG 0.479560 4.2:+40903313 None:intergenic
TGATTAACATGATCAGAAAC+AGG 0.483590 4.2:+40904057 None:intergenic
TGCCACTGTTTATATGTCTC+GGG 0.483709 4.2:+40902581 None:intergenic
CCGCAGCCTCAACTCTTGAC+TGG 0.485147 4.2:-40903862 MS.gene071190:CDS
GAGGAGTCACCTCCATCAAA+TGG 0.486751 4.2:-40903583 MS.gene071190:CDS
CAAGCTAGTGATTTCACAAA+CGG 0.489712 4.2:-40904531 MS.gene071190:CDS
GAATGAGGTTGAGAAATAGC+AGG 0.490215 4.2:+40904015 None:intergenic
TTGGCTCATTACTTTGCCAA+TGG 0.490767 4.2:-40903391 MS.gene071190:CDS
TTGGACGAAAACCAGGTAAA+GGG 0.491920 4.2:+40903065 None:intergenic
ACAATTGGATGCTTCAAGGT+TGG 0.492282 4.2:-40902442 MS.gene071190:CDS
AAGAACAAGAAGAAGAAGCT+TGG 0.493244 4.2:-40903744 MS.gene071190:CDS
CTTTCCATTGGACGAAAACC+AGG 0.495527 4.2:+40903058 None:intergenic
GAAAATGATTCCTTGCAGAA+TGG 0.503064 4.2:-40903619 MS.gene071190:CDS
TTGGCCACTGAAAACCATAT+AGG 0.503360 4.2:+40903155 None:intergenic
TGGGGATGGACCACAGAGGT+TGG 0.505585 4.2:-40903410 MS.gene071190:CDS
CCGGTGATCTTCAGGTTTGG+AGG 0.507786 4.2:+40903097 None:intergenic
TTCCCAATAACGCGATGGTA+TGG 0.510255 4.2:+40902967 None:intergenic
GCAGAATGGACAAGTGAAAA+CGG 0.510872 4.2:-40903605 MS.gene071190:CDS
AAGGCGTGCCTCAAGGCCAT+TGG 0.511609 4.2:+40903375 None:intergenic
TATAGAAGGTTATATGGCCT+CGG 0.515236 4.2:-40904364 MS.gene071190:CDS
CATTCTTCACCCTCTGGGGA+TGG 0.516297 4.2:-40903424 MS.gene071190:CDS
TTGGGAAACAATTCGAGTTG+AGG 0.525214 4.2:-40902951 MS.gene071190:CDS
CGCCGACAAGGCGTGCCTCA+AGG 0.525318 4.2:+40903368 None:intergenic
CTAAACCGTGTAGCAAAGAA+TGG 0.525919 4.2:+40902755 None:intergenic
TCAGTCATTTGTAAGCTAAG+TGG 0.527232 4.2:+40904240 None:intergenic
GGATTCTTCTATTACACAGA+TGG 0.528072 4.2:-40903962 MS.gene071190:CDS
GGTATGGCATTAAACTCAAA+GGG 0.528598 4.2:+40902983 None:intergenic
ATTGGACGAAAACCAGGTAA+AGG 0.530391 4.2:+40903064 None:intergenic
CATTAAACTCAAAGGGAACA+TGG 0.533197 4.2:+40902990 None:intergenic
ATTGATTTGGATCAGCATCA+AGG 0.534036 4.2:+40904439 None:intergenic
AATGAGGTTGAGAAATAGCA+GGG 0.534181 4.2:+40904016 None:intergenic
CGAGACATATAAACAGTGGC+AGG 0.537307 4.2:-40902579 MS.gene071190:CDS
AAACAGGAGTATCAGGTGAA+AGG 0.542644 4.2:+40904073 None:intergenic
GTCAAGAGTTGAGGCTGCGG+AGG 0.544335 4.2:+40903865 None:intergenic
GAGAATCCGTTTGTGTTGAA+GGG 0.545933 4.2:-40903802 MS.gene071190:CDS
CTCCAGTACCATCGCCGACA+AGG 0.546196 4.2:+40903356 None:intergenic
TTAGATATTCATAACTCTGA+TGG 0.546938 4.2:-40904156 MS.gene071190:CDS
GTTCATAAACCAAATCCTGA+TGG 0.550468 4.2:-40904295 MS.gene071190:CDS
TACCACGCGAAAACAAATAC+AGG 0.550992 4.2:-40902682 MS.gene071190:CDS
TCAGAGTTATGAATATCTAA+GGG 0.551258 4.2:+40904159 None:intergenic
TACCAAAATCAATGATATGG+AGG 0.553791 4.2:+40903179 None:intergenic
CTCCAAACCTGAAGATCACC+GGG 0.554977 4.2:-40903096 MS.gene071190:CDS
TGGTATGGCATTAAACTCAA+AGG 0.555962 4.2:+40902982 None:intergenic
AGGTAAAGGGAACTCTATCC+CGG 0.560031 4.2:+40903078 None:intergenic
CCTCCAAACCTGAAGATCAC+CGG 0.561178 4.2:-40903097 MS.gene071190:CDS
AAATCAGTGTCTTCGAGGGA+AGG 0.562271 4.2:+40904333 None:intergenic
GAGGCACGCCTTGTCGGCGA+TGG 0.563864 4.2:-40903364 MS.gene071190:CDS
ATGAACGTTGTAGCTTGTGA+AGG 0.570356 4.2:-40902623 MS.gene071190:CDS
CATTCTACTGGTGTTAGTGA+TGG 0.570556 4.2:-40903997 MS.gene071190:CDS
CCAGTCAAGAGTTGAGGCTG+CGG 0.571987 4.2:+40903862 None:intergenic
ATTCGCCATTCTTTGCTACA+CGG 0.575217 4.2:-40902760 MS.gene071190:CDS
TGGATGCTTCAAGGTTGGAA+AGG 0.577566 4.2:-40902437 MS.gene071190:CDS
TCTTGGTGTTCGGAGAGGAA+TGG 0.578017 4.2:+40903323 None:intergenic
CTCAGCAGTAGAGATCCTCT+TGG 0.580706 4.2:+40903306 None:intergenic
GTGATGTGAGTTGATTATGC+TGG 0.583220 4.2:+40902383 None:intergenic
TACTCCCTTCTTCACCAAAG+TGG 0.586539 4.2:+40904502 None:intergenic
ACTACCACTTTGGTGAAGAA+GGG 0.587510 4.2:-40904506 MS.gene071190:CDS
CATATAACCTTCTATAGGGA+AGG 0.589911 4.2:+40904371 None:intergenic
CTTTGCTACACGGTTTAGGG+AGG 0.591419 4.2:-40902750 MS.gene071190:CDS
ATGGAAGCGCTTGCAATAGT+CGG 0.593097 4.2:+40903009 None:intergenic
TTACTTTGCCAATGGCCTTG+AGG 0.593344 4.2:-40903383 MS.gene071190:CDS
AAACAAATACAGGATGCTGA+TGG 0.598030 4.2:-40902672 MS.gene071190:CDS
CAATTTGGATATGGTTAGGG+AGG 0.598257 4.2:-40903827 MS.gene071190:CDS
CTCTGTGGTCCATCCCCAGA+GGG 0.598960 4.2:+40903415 None:intergenic
ACCTTCTATAGGGAAGGTGG+TGG 0.600256 4.2:+40904377 None:intergenic
TTTCACTTGTCCATTCTGCA+AGG 0.603209 4.2:+40903609 None:intergenic
ACCAAAATCAATGATATGGA+GGG 0.610467 4.2:+40903180 None:intergenic
ATAACCTTCTATAGGGAAGG+TGG 0.616055 4.2:+40904374 None:intergenic
ATACAGGATGCTGATGGAGA+GGG 0.620955 4.2:-40902666 MS.gene071190:CDS
AAGCTAAGTGGGTCATAGAA+CGG 0.623038 4.2:+40904252 None:intergenic
ATTGTTTCCCAATAACGCGA+TGG 0.623674 4.2:+40902962 None:intergenic
GTCTTCCCTCCATTTGATGG+AGG 0.626646 4.2:+40903574 None:intergenic
GAGTCACCTCCATCAAATGG+AGG 0.628730 4.2:-40903580 MS.gene071190:CDS
TCACTAACACCAGTAGAATG+AGG 0.628786 4.2:+40904000 None:intergenic
TCAAAACCAGTCAAGAGTTG+AGG 0.630836 4.2:+40903856 None:intergenic
AATACAGGATGCTGATGGAG+AGG 0.637418 4.2:-40902667 MS.gene071190:CDS
ACAGGAGTATCAGGTGAAAG+GGG 0.642453 4.2:+40904075 None:intergenic
ATTCCTCTCCGAACACCAAG+AGG 0.643154 4.2:-40903321 MS.gene071190:CDS
AGTCACCTCCATCAAATGGA+GGG 0.643984 4.2:-40903579 MS.gene071190:CDS
CCTCTGTGGTCCATCCCCAG+AGG 0.644825 4.2:+40903414 None:intergenic
GCAACATTCTTCACCCTCTG+GGG 0.646104 4.2:-40903428 MS.gene071190:CDS
TTATGCTGGCACCCAACATG+TGG 0.647174 4.2:+40902397 None:intergenic
AAGGTTTAGAGAGAGTTGAG+AGG 0.649221 4.2:-40902604 MS.gene071190:CDS
AAGACTCGTCGTAAGAAACA+AGG 0.649427 4.2:-40903556 MS.gene071190:CDS
ATGAGGTTGAGAAATAGCAG+GGG 0.658762 4.2:+40904017 None:intergenic
GATCCTCTTGGTGTTCGGAG+AGG 0.659641 4.2:+40903318 None:intergenic
AGCTATTAGTGCTATTGACG+AGG 0.669842 4.2:-40903704 MS.gene071190:CDS
CAGAGTTATGAATATCTAAG+GGG 0.672632 4.2:+40904160 None:intergenic
CAACAGCAATAGCAGCGTCG+AGG 0.673827 4.2:-40904115 MS.gene071190:CDS
TTTGTGAAATCACTAGCTTG+AGG 0.676421 4.2:+40904534 None:intergenic
AAGTAATGAGCCAACCTCTG+TGG 0.682694 4.2:+40903400 None:intergenic
GAGGGAAGGATCCGTCGCCG+AGG 0.685761 4.2:+40904347 None:intergenic
TCTTAAAATCAAGAGCAATG+AGG 0.688807 4.2:-40902927 MS.gene071190:CDS
GTGGTGGCTGCAAAACCAAG+TGG 0.689009 4.2:+40904393 None:intergenic
AGCGAACAGTCCTAGAAATG+CGG 0.690521 4.2:-40902849 MS.gene071190:CDS
GGCCCGAGACATATAAACAG+TGG 0.698214 4.2:-40902583 MS.gene071190:CDS
CTGATGGAGAGGGAAAGTAT+CGG 0.714910 4.2:-40902656 MS.gene071190:CDS
TGGTGAAGAAGGGAGTACAG+TGG 0.749828 4.2:-40904496 MS.gene071190:CDS
GAATGGACAAGTGAAAACGG+AGG 0.763230 4.2:-40903602 MS.gene071190:CDS
CCTCTGGGGATGGACCACAG+AGG 0.797161 4.2:-40903414 MS.gene071190:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! GATAAAAACTTTATAAGTAT+TGG + chr4.2:40903813-40903832 None:intergenic 15.0%
!! GATGAGATTTAAGAATTTAT+TGG - chr4.2:40904061-40904080 MS.gene071190:CDS 20.0%
!!! TTCTAAACTTTTCTTGAATA+GGG + chr4.2:40903043-40903062 None:intergenic 20.0%
! AATCAATATGATAGCTTTCT+TGG - chr4.2:40902523-40902542 MS.gene071190:CDS 25.0%
! AGAATGAAACAATAGATTTG+AGG - chr4.2:40903418-40903437 MS.gene071190:CDS 25.0%
! ATCAGAGTTATGAATATCTA+AGG + chr4.2:40902791-40902810 None:intergenic 25.0%
! ATTATCAGAGATGTTTGATA+AGG - chr4.2:40903269-40903288 MS.gene071190:CDS 25.0%
! TCAGAGTTATGAATATCTAA+GGG + chr4.2:40902790-40902809 None:intergenic 25.0%
! TTAGATATTCATAACTCTGA+TGG - chr4.2:40902790-40902809 MS.gene071190:CDS 25.0%
! TTCAAGAAAAGTTTAGAAGA+AGG - chr4.2:40903045-40903064 MS.gene071190:CDS 25.0%
! TTTGTTCAGTCAATTGTTAA+TGG - chr4.2:40904155-40904174 MS.gene071190:CDS 25.0%
!! AAGTATGCAAACTTTTTGAA+AGG + chr4.2:40903702-40903721 None:intergenic 25.0%
!! AAGTTTTTATCAGATAGAGA+AGG - chr4.2:40903822-40903841 MS.gene071190:CDS 25.0%
!! AGAAAGCTATCATATTGATT+TGG + chr4.2:40902523-40902542 None:intergenic 25.0%
!! CTTCTAAACTTTTCTTGAAT+AGG + chr4.2:40903044-40903063 None:intergenic 25.0%
!! GAATCATTTTCATTGCATAA+TGG + chr4.2:40903318-40903337 None:intergenic 25.0%
!! TTTTTGATGTAGACAACAAT+TGG - chr4.2:40904489-40904508 MS.gene071190:CDS 25.0%
!!! ATTGATTTTGGTATCCTATA+TGG - chr4.2:40903777-40903796 MS.gene071190:CDS 25.0%
ACCAAAATCAATGATATGGA+GGG + chr4.2:40903769-40903788 None:intergenic 30.0%
ATCCAATTTGGATATGGTTA+GGG - chr4.2:40903116-40903135 MS.gene071190:CDS 30.0%
ATTGACTGAACAAAAATAGC+AGG + chr4.2:40904149-40904168 None:intergenic 30.0%
CAGAGTTATGAATATCTAAG+GGG + chr4.2:40902789-40902808 None:intergenic 30.0%
CATCAAAAACAAAGTCTTTG+TGG + chr4.2:40904478-40904497 None:intergenic 30.0%
GGATACCAAAATCAATGATA+TGG + chr4.2:40903773-40903792 None:intergenic 30.0%
TACCAAAATCAATGATATGG+AGG + chr4.2:40903770-40903789 None:intergenic 30.0%
TCCATTAATTCCGAATTCAA+TGG + chr4.2:40904425-40904444 None:intergenic 30.0%
TCCATTGAATTCGGAATTAA+TGG - chr4.2:40904421-40904440 MS.gene071190:CDS 30.0%
TCTTAAAATCAAGAGCAATG+AGG - chr4.2:40904019-40904038 MS.gene071190:CDS 30.0%
TGAATCATCCAATTTGGATA+TGG - chr4.2:40903110-40903129 MS.gene071190:CDS 30.0%
TGATTAACATGATCAGAAAC+AGG + chr4.2:40902892-40902911 None:intergenic 30.0%
TTTATATGCTTCCACATGTT+GGG - chr4.2:40904538-40904557 MS.gene071190:CDS 30.0%
! AGGAAGCTATCATATTGATT+TGG + chr4.2:40902490-40902509 None:intergenic 30.0%
! CGGAATTAATGGACAAATTT+AGG - chr4.2:40904432-40904451 MS.gene071190:CDS 30.0%
! TTTTATATGCTTCCACATGT+TGG - chr4.2:40904537-40904556 MS.gene071190:CDS 30.0%
! TTTTTATCAGATAGAGAAGG+TGG - chr4.2:40903825-40903844 MS.gene071190:CDS 30.0%
!!! TGGTTTTGAATCATCCAATT+TGG - chr4.2:40903104-40903123 MS.gene071190:CDS 30.0%
AAACAAATACAGGATGCTGA+TGG - chr4.2:40904274-40904293 MS.gene071190:CDS 35.0%
AAGAACAAGAAGAAGAAGCT+TGG - chr4.2:40903202-40903221 MS.gene071190:CDS 35.0%
AATGAGGTTGAGAAATAGCA+GGG + chr4.2:40902933-40902952 None:intergenic 35.0%
AGGCCATATAACCTTCTATA+GGG + chr4.2:40902582-40902601 None:intergenic 35.0%
ATGGAAAGAATCGAAGAAAC+TGG - chr4.2:40903903-40903922 MS.gene071190:CDS 35.0%
ATTAGCATTCCTGTTGTCAT+TGG + chr4.2:40903478-40903497 None:intergenic 35.0%
ATTCTTTGCTACACGGTTTA+GGG - chr4.2:40904193-40904212 MS.gene071190:CDS 35.0%
ATTTCTCAACCTCATTCTAC+TGG - chr4.2:40902937-40902956 MS.gene071190:CDS 35.0%
CAAACAGCTTCCATTGAATT+CGG - chr4.2:40904412-40904431 MS.gene071190:CDS 35.0%
CAAGCTAGTGATTTCACAAA+CGG - chr4.2:40902415-40902434 MS.gene071190:CDS 35.0%
CAGTCATTTGTAAGCTAAGT+GGG + chr4.2:40902708-40902727 None:intergenic 35.0%
CATATAACCTTCTATAGGGA+AGG + chr4.2:40902578-40902597 None:intergenic 35.0%
CATCCAATTTGGATATGGTT+AGG - chr4.2:40903115-40903134 MS.gene071190:CDS 35.0%
CATTAAACTCAAAGGGAACA+TGG + chr4.2:40903959-40903978 None:intergenic 35.0%
CTTCCCTATAGAAGGTTATA+TGG - chr4.2:40902576-40902595 MS.gene071190:CDS 35.0%
GAAAATGATTCCTTGCAGAA+TGG - chr4.2:40903327-40903346 MS.gene071190:CDS 35.0%
GGATTCTTCTATTACACAGA+TGG - chr4.2:40902984-40903003 MS.gene071190:CDS 35.0%
GGTATGGCATTAAACTCAAA+GGG + chr4.2:40903966-40903985 None:intergenic 35.0%
GTTCATAAACCAAATCCTGA+TGG - chr4.2:40902651-40902670 MS.gene071190:CDS 35.0%
GTTTCTTCGATTCTTTCCAT+TGG + chr4.2:40903903-40903922 None:intergenic 35.0%
TATAGAAGGTTATATGGCCT+CGG - chr4.2:40902582-40902601 MS.gene071190:CDS 35.0%
TCAGTCATTTGTAAGCTAAG+TGG + chr4.2:40902709-40902728 None:intergenic 35.0%
TGAAAAATCAGTGTCTTCGA+GGG + chr4.2:40902620-40902639 None:intergenic 35.0%
TGGTATGGCATTAAACTCAA+AGG + chr4.2:40903967-40903986 None:intergenic 35.0%
TTTCTACCCTTCAACACAAA+CGG + chr4.2:40903153-40903172 None:intergenic 35.0%
! CATTTTCTTCCATCAGGATT+TGG + chr4.2:40902663-40902682 None:intergenic 35.0%
! GATAAGGTTTTGCTTGATGT+CGG - chr4.2:40903285-40903304 MS.gene071190:CDS 35.0%
! GATCAACATTTTCTTCCATC+AGG + chr4.2:40902669-40902688 None:intergenic 35.0%
! GTATCCTATATGGTTTTCAG+TGG - chr4.2:40903787-40903806 MS.gene071190:CDS 35.0%
! TTTGTGAAATCACTAGCTTG+AGG + chr4.2:40902415-40902434 None:intergenic 35.0%
!! ATATCTAAGGGGTGTTGATT+AGG + chr4.2:40902778-40902797 None:intergenic 35.0%
!! ATTGATTTGGATCAGCATCA+AGG + chr4.2:40902510-40902529 None:intergenic 35.0%
!!! ACCCTCCATATCATTGATTT+TGG - chr4.2:40903765-40903784 MS.gene071190:CDS 35.0%
!!! GACACTGATTTTTCAGATAC+TGG - chr4.2:40902625-40902644 MS.gene071190:CDS 35.0%
AAATGCAGAACCGCATTTCT+AGG + chr4.2:40904110-40904129 None:intergenic 40.0%
AAGACTCGTCGTAAGAAACA+AGG - chr4.2:40903390-40903409 MS.gene071190:CDS 40.0%
AAGCTAAGTGGGTCATAGAA+CGG + chr4.2:40902697-40902716 None:intergenic 40.0%
AAGGTTTAGAGAGAGTTGAG+AGG - chr4.2:40904342-40904361 MS.gene071190:CDS 40.0%
ACAAACGGTTACTACCACTT+TGG - chr4.2:40902430-40902449 MS.gene071190:CDS 40.0%
ACAATTGGATGCTTCAAGGT+TGG - chr4.2:40904504-40904523 MS.gene071190:CDS 40.0%
ACTACCACTTTGGTGAAGAA+GGG - chr4.2:40902440-40902459 MS.gene071190:CDS 40.0%
AGAGAATCCGTTTGTGTTGA+AGG - chr4.2:40903143-40903162 MS.gene071190:CDS 40.0%
ATAACCTTCTATAGGGAAGG+TGG + chr4.2:40902575-40902594 None:intergenic 40.0%
ATGAGCTACTGAAGCAGATT+AGG - chr4.2:40903496-40903515 MS.gene071190:CDS 40.0%
ATGAGGTTGAGAAATAGCAG+GGG + chr4.2:40902932-40902951 None:intergenic 40.0%
ATTCGCCATTCTTTGCTACA+CGG - chr4.2:40904186-40904205 MS.gene071190:CDS 40.0%
ATTGGACGAAAACCAGGTAA+AGG + chr4.2:40903885-40903904 None:intergenic 40.0%
ATTGTTTCCCAATAACGCGA+TGG + chr4.2:40903987-40904006 None:intergenic 40.0%
CAATTTGGATATGGTTAGGG+AGG - chr4.2:40903119-40903138 MS.gene071190:CDS 40.0%
CATATAAACAGTGGCAGGTT+AGG - chr4.2:40904372-40904391 MS.gene071190:CDS 40.0%
CATTCTTTGCTACACGGTTT+AGG - chr4.2:40904192-40904211 MS.gene071190:CDS 40.0%
CTAAACCGTGTAGCAAAGAA+TGG + chr4.2:40904194-40904213 None:intergenic 40.0%
CTCCCTAACCATATCCAAAT+TGG + chr4.2:40903121-40903140 None:intergenic 40.0%
CTGAAAAATCAGTGTCTTCG+AGG + chr4.2:40902621-40902640 None:intergenic 40.0%
CTGCCACTGTTTATATGTCT+CGG + chr4.2:40904369-40904388 None:intergenic 40.0%
CTGTATATGCCAATGACAAC+AGG - chr4.2:40903466-40903485 MS.gene071190:CDS 40.0%
GAATGAGGTTGAGAAATAGC+AGG + chr4.2:40902934-40902953 None:intergenic 40.0%
GACAACAATTGGATGCTTCA+AGG - chr4.2:40904500-40904519 MS.gene071190:CDS 40.0%
GAGAATCCGTTTGTGTTGAA+GGG - chr4.2:40903144-40903163 MS.gene071190:CDS 40.0%
GAGGCCATATAACCTTCTAT+AGG + chr4.2:40902583-40902602 None:intergenic 40.0%
GCAGAATGGACAAGTGAAAA+CGG - chr4.2:40903341-40903360 MS.gene071190:CDS 40.0%
GTGGTAACATTGCTGTAACT+TGG + chr4.2:40904459-40904478 None:intergenic 40.0%
TACCACGCGAAAACAAATAC+AGG - chr4.2:40904264-40904283 MS.gene071190:CDS 40.0%
TACTACCACTTTGGTGAAGA+AGG - chr4.2:40902439-40902458 MS.gene071190:CDS 40.0%
TCAAAACCAGTCAAGAGTTG+AGG + chr4.2:40903093-40903112 None:intergenic 40.0%
TCACTAACACCAGTAGAATG+AGG + chr4.2:40902949-40902968 None:intergenic 40.0%
TGCCACTGTTTATATGTCTC+GGG + chr4.2:40904368-40904387 None:intergenic 40.0%
TTGGACGAAAACCAGGTAAA+GGG + chr4.2:40903884-40903903 None:intergenic 40.0%
TTGGCCACTGAAAACCATAT+AGG + chr4.2:40903794-40903813 None:intergenic 40.0%
TTGGCTCATTACTTTGCCAA+TGG - chr4.2:40903555-40903574 MS.gene071190:CDS 40.0%
TTTCACTTGTCCATTCTGCA+AGG + chr4.2:40903340-40903359 None:intergenic 40.0%
! AGCTATTAGTGCTATTGACG+AGG - chr4.2:40903242-40903261 MS.gene071190:CDS 40.0%
! ATGAACGTTGTAGCTTGTGA+AGG - chr4.2:40904323-40904342 MS.gene071190:CDS 40.0%
! CATTCTACTGGTGTTAGTGA+TGG - chr4.2:40902949-40902968 MS.gene071190:CDS 40.0%
! TCACTAGCTTGAGGAAAGTT+TGG + chr4.2:40902406-40902425 None:intergenic 40.0%
!! AAACAGGAGTATCAGGTGAA+AGG + chr4.2:40902876-40902895 None:intergenic 40.0%
!! AACAGGAGTATCAGGTGAAA+GGG + chr4.2:40902875-40902894 None:intergenic 40.0%
!! ATCCTGTATTTGTTTTCGCG+TGG + chr4.2:40904269-40904288 None:intergenic 40.0%
!! TAGTGATGGACTCAGAGATT+TGG - chr4.2:40902963-40902982 MS.gene071190:CDS 40.0%
!! TGATCAGAAACAGGAGTATC+AGG + chr4.2:40902883-40902902 None:intergenic 40.0%
!! TTGGGAAACAATTCGAGTTG+AGG - chr4.2:40903995-40904014 MS.gene071190:CDS 40.0%
!! TTTACCTGGTTTTCGTCCAA+TGG - chr4.2:40903884-40903903 MS.gene071190:CDS 40.0%
AAATCAGTGTCTTCGAGGGA+AGG + chr4.2:40902616-40902635 None:intergenic 45.0%
AAGTAATGAGCCAACCTCTG+TGG + chr4.2:40903549-40903568 None:intergenic 45.0%
AATACAGGATGCTGATGGAG+AGG - chr4.2:40904279-40904298 MS.gene071190:CDS 45.0%
AGCGAACAGTCCTAGAAATG+CGG - chr4.2:40904097-40904116 MS.gene071190:CDS 45.0%
AGGTAAAGGGAACTCTATCC+CGG + chr4.2:40903871-40903890 None:intergenic 45.0%
AGTCACCTCCATCAAATGGA+GGG - chr4.2:40903367-40903386 MS.gene071190:CDS 45.0%
ATACAGGATGCTGATGGAGA+GGG - chr4.2:40904280-40904299 MS.gene071190:CDS 45.0%
ATGCCATACCATCGCGTTAT+TGG - chr4.2:40903976-40903995 MS.gene071190:CDS 45.0%
CGAGACATATAAACAGTGGC+AGG - chr4.2:40904367-40904386 MS.gene071190:CDS 45.0%
CTTTCCATTGGACGAAAACC+AGG + chr4.2:40903891-40903910 None:intergenic 45.0%
GAATGGACAAGTGAAAACGG+AGG - chr4.2:40903344-40903363 MS.gene071190:CDS 45.0%
TACTCCCTTCTTCACCAAAG+TGG + chr4.2:40902447-40902466 None:intergenic 45.0%
TGCCATACCATCGCGTTATT+GGG - chr4.2:40903977-40903996 MS.gene071190:CDS 45.0%
TGGATGCTTCAAGGTTGGAA+AGG - chr4.2:40904509-40904528 MS.gene071190:CDS 45.0%
TTCCCAATAACGCGATGGTA+TGG + chr4.2:40903982-40904001 None:intergenic 45.0%
! CTGATGGAGAGGGAAAGTAT+CGG - chr4.2:40904290-40904309 MS.gene071190:CDS 45.0%
! TTACTTTGCCAATGGCCTTG+AGG - chr4.2:40903563-40903582 MS.gene071190:CDS 45.0%
!! ACAGGAGTATCAGGTGAAAG+GGG + chr4.2:40902874-40902893 None:intergenic 45.0%
!! ATGGAAGCGCTTGCAATAGT+CGG + chr4.2:40903940-40903959 None:intergenic 45.0%
!! GGGTGTTGATTAGGAGAAAG+AGG + chr4.2:40902769-40902788 None:intergenic 45.0%
!! GTAGAGATCCTCTTGGTGTT+CGG + chr4.2:40903636-40903655 None:intergenic 45.0%
!! TTTCTTGGTGCTGATCCACT+TGG - chr4.2:40902538-40902557 MS.gene071190:CDS 45.0%
ACCTTCTATAGGGAAGGTGG+TGG + chr4.2:40902572-40902591 None:intergenic 50.0%
ACTCTATCCCGGTGATCTTC+AGG + chr4.2:40903860-40903879 None:intergenic 50.0%
AGGCAACATTCTTCACCCTC+TGG - chr4.2:40903516-40903535 MS.gene071190:CDS 50.0%
ATTCCTCTCCGAACACCAAG+AGG - chr4.2:40903625-40903644 MS.gene071190:CDS 50.0%
CCTCCAAACCTGAAGATCAC+CGG - chr4.2:40903849-40903868 MS.gene071190:CDS 50.0%
CGAGTCTTCCCTCCATTTGA+TGG + chr4.2:40903378-40903397 None:intergenic 50.0%
CTCAGCAGTAGAGATCCTCT+TGG + chr4.2:40903643-40903662 None:intergenic 50.0%
CTCCAAACCTGAAGATCACC+GGG - chr4.2:40903850-40903869 MS.gene071190:CDS 50.0%
CTTTGCTACACGGTTTAGGG+AGG - chr4.2:40904196-40904215 MS.gene071190:CDS 50.0%
GAGGAGTCACCTCCATCAAA+TGG - chr4.2:40903363-40903382 MS.gene071190:CDS 50.0%
GAGTCACCTCCATCAAATGG+AGG - chr4.2:40903366-40903385 MS.gene071190:CDS 50.0%
GCAACATTCTTCACCCTCTG+GGG - chr4.2:40903518-40903537 MS.gene071190:CDS 50.0%
GGCAACATTCTTCACCCTCT+GGG - chr4.2:40903517-40903536 MS.gene071190:CDS 50.0%
GGCCCGAGACATATAAACAG+TGG - chr4.2:40904363-40904382 MS.gene071190:CDS 50.0%
GGGATAGAGTTCCCTTTACC+TGG - chr4.2:40903870-40903889 MS.gene071190:CDS 50.0%
GTCTTCCCTCCATTTGATGG+AGG + chr4.2:40903375-40903394 None:intergenic 50.0%
TTATGCTGGCACCCAACATG+TGG + chr4.2:40904552-40904571 None:intergenic 50.0%
! ATCCCGGTGATCTTCAGGTT+TGG + chr4.2:40903855-40903874 None:intergenic 50.0%
! ATTGCTGTTGCTGTTGCTGC+TGG + chr4.2:40902821-40902840 None:intergenic 50.0%
! TGGTGAAGAAGGGAGTACAG+TGG - chr4.2:40902450-40902469 MS.gene071190:CDS 50.0%
!! TCTTGGTGTTCGGAGAGGAA+TGG + chr4.2:40903626-40903645 None:intergenic 50.0%
AGGTTATATGGCCTCGGCGA+CGG - chr4.2:40902588-40902607 MS.gene071190:CDS 55.0%
CAACAGCAATAGCAGCGTCG+AGG - chr4.2:40902831-40902850 MS.gene071190:CDS 55.0%
CCAGTCAAGAGTTGAGGCTG+CGG + chr4.2:40903087-40903106 None:intergenic 55.0%
GCCACCACCTTCCCTATAGA+AGG - chr4.2:40902568-40902587 MS.gene071190:CDS 55.0%
GTGGTGGCTGCAAAACCAAG+TGG + chr4.2:40902556-40902575 None:intergenic 55.0%
! CATTCTTCACCCTCTGGGGA+TGG - chr4.2:40903522-40903541 MS.gene071190:CDS 55.0%
! CCGGTGATCTTCAGGTTTGG+AGG + chr4.2:40903852-40903871 None:intergenic 55.0%
!! GATCCTCTTGGTGTTCGGAG+AGG + chr4.2:40903631-40903650 None:intergenic 55.0%
AAGGCGTGCCTCAAGGCCAT+TGG + chr4.2:40903574-40903593 None:intergenic 60.0%
CCGCAGCCTCAACTCTTGAC+TGG - chr4.2:40903084-40903103 MS.gene071190:CDS 60.0%
CTCCAGTACCATCGCCGACA+AGG + chr4.2:40903593-40903612 None:intergenic 60.0%
CTCTGTGGTCCATCCCCAGA+GGG + chr4.2:40903534-40903553 None:intergenic 60.0%
GTCAAGAGTTGAGGCTGCGG+AGG + chr4.2:40903084-40903103 None:intergenic 60.0%
TGGGGATGGACCACAGAGGT+TGG - chr4.2:40903536-40903555 MS.gene071190:CDS 60.0%
CCTCTGTGGTCCATCCCCAG+AGG + chr4.2:40903535-40903554 None:intergenic 65.0%
CGCCTTGTCGGCGATGGTAC+TGG - chr4.2:40903588-40903607 MS.gene071190:CDS 65.0%
GGCCTTGAGGCACGCCTTGT+CGG - chr4.2:40903576-40903595 MS.gene071190:CDS 65.0%
! CCTCTGGGGATGGACCACAG+AGG - chr4.2:40903532-40903551 MS.gene071190:CDS 65.0%
CGCCGACAAGGCGTGCCTCA+AGG + chr4.2:40903581-40903600 None:intergenic 70.0%
GAGGCACGCCTTGTCGGCGA+TGG - chr4.2:40903582-40903601 MS.gene071190:CDS 70.0%
GAGGGAAGGATCCGTCGCCG+AGG + chr4.2:40902602-40902621 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 40902397 40904571 40902397 ID=MS.gene071190
chr4.2 mRNA 40902397 40904571 40902397 ID=MS.gene071190.t1;Parent=MS.gene071190
chr4.2 exon 40902397 40904571 40902397 ID=MS.gene071190.t1.exon1;Parent=MS.gene071190.t1
chr4.2 CDS 40902397 40904571 40902397 ID=cds.MS.gene071190.t1;Parent=MS.gene071190.t1
Gene Sequence

>MS.gene071190

ATGGATCCAAACTTTCCTCAAGCTAGTGATTTCACAAACGGTTACTACCACTTTGGTGAAGAAGGGAGTACAGTGGTCTCTGAGTTTGATCCAAATCAATATGATAGCTTCCTTGATGCTGATCCAAATCAATATGATAGCTTTCTTGGTGCTGATCCACTTGGTTTTGCAGCCACCACCTTCCCTATAGAAGGTTATATGGCCTCGGCGACGGATCCTTCCCTCGAAGACACTGATTTTTCAGATACTGGTAAGTTCATAAACCAAATCCTGATGGAAGAAAATGTTGATCAGAGACCGTTCTATGACCCACTTAGCTTACAAATGACTGAAAAATCATTCTATGATGCTCTTTTACACACAAATAAACCTCTTTCTCCTAATCAACACCCCTTAGATATTCATAACTCTGATGGCACAACCAGCAGCAACAGCAACAGCAATAGCAGCGTCGAGGATTCTCGAGAGTTAAAACCCCTTTCACCTGATACTCCTGTTTCTGATCATGTTAATCATGTTTTTCAGTTTAATTCCCCTGCTATTTCTCAACCTCATTCTACTGGTGTTAGTGATGGACTCAGAGATTTGGATTCTTCTATTACACAGATGGCACAAAACATTTTCAGTGATGTAGATTCTGTTTCCCTATTCAAGAAAAGTTTAGAAGAAGGCAACAAGTTTCTTCCTCCGCAGCCTCAACTCTTGACTGGTTTTGAATCATCCAATTTGGATATGGTTAGGGAGGAAGAGAATCCGTTTGTGTTGAAGGGTAGAAAGAATCACAAGCGCGAAGAAAGTTTTAGTAAAGAACAAGAAGAAGAAGCTTGGAGAAGTAGCAAGCAATCAGCTATTAGTGCTATTGACGAGGATGAATTATCAGAGATGTTTGATAAGGTTTTGCTTGATGTCGGACATTTGCCATTATGCAATGAAAATGATTCCTTGCAGAATGGACAAGTGAAAACGGAGGAGTCACCTCCATCAAATGGAGGGAAGACTCGTCGTAAGAAACAAGGCAAGAAGAATGAAACAATAGATTTGAGGAATCTTCTGCTTATGTGTTCACAATCTGTATATGCCAATGACAACAGGAATGCTAATGAGCTACTGAAGCAGATTAGGCAACATTCTTCACCCTCTGGGGATGGACCACAGAGGTTGGCTCATTACTTTGCCAATGGCCTTGAGGCACGCCTTGTCGGCGATGGTACTGGAGCACAAACATTCCATTCCTCTCCGAACACCAAGAGGATCTCTACTGCTGAGTTTTTGAAAGCATATCAAGTTCATCTATCTACTAGTCCTTTCAAAAAGTTTGCATACTTCTTTGCAAATAAAATGATTATGAAAGCTTCTGCAAATGCTGAAACCCTCCATATCATTGATTTTGGTATCCTATATGGTTTTCAGTGGCCAATACTTATAAAGTTTTTATCAGATAGAGAAGGTGGACCTCCAAACCTGAAGATCACCGGGATAGAGTTCCCTTTACCTGGTTTTCGTCCAATGGAAAGAATCGAAGAAACTGGTCGTCGTCTTGCCGACTATTGCAAGCGCTTCCATGTTCCCTTTGAGTTTAATGCCATACCATCGCGTTATTGGGAAACAATTCGAGTTGAGGATCTTAAAATCAAGAGCAATGAGGTTGTTGTTGTGAACTCTTTGATGAGATTTAAGAATTTATTGGATGAAAGTATTGAAGCGAACAGTCCTAGAAATGCGGTTCTGCATTTAATCAGAAAGATAAATCCTGCTATTTTTGTTCAGTCAATTGTTAATGGATCATATAATTCGCCATTCTTTGCTACACGGTTTAGGGAGGCGCTGTTTCATTTTTCTGCTCTTTATGATATGTTTGACACTGTGATACCACGCGAAAACAAATACAGGATGCTGATGGAGAGGGAAAGTATCGGTAGAGAAGCTATGAACGTTGTAGCTTGTGAAGGTTTAGAGAGAGTTGAGAGGCCCGAGACATATAAACAGTGGCAGGTTAGGAATACAAGAGCTGATTTCAAACAGCTTCCATTGAATTCGGAATTAATGGACAAATTTAGGACCAAGTTACAGCAATGTTACCACAAAGACTTTGTTTTTGATGTAGACAACAATTGGATGCTTCAAGGTTGGAAAGGACGCATTTTATATGCTTCCACATGTTGGGTGCCAGCATAA
Protein sequence

>MS.gene071190.t1

MDPNFPQASDFTNGYYHFGEEGSTVVSEFDPNQYDSFLDADPNQYDSFLGADPLGFAATTFPIEGYMASATDPSLEDTDFSDTGKFINQILMEENVDQRPFYDPLSLQMTEKSFYDALLHTNKPLSPNQHPLDIHNSDGTTSSNSNSNSSVEDSRELKPLSPDTPVSDHVNHVFQFNSPAISQPHSTGVSDGLRDLDSSITQMAQNIFSDVDSVSLFKKSLEEGNKFLPPQPQLLTGFESSNLDMVREEENPFVLKGRKNHKREESFSKEQEEEAWRSSKQSAISAIDEDELSEMFDKVLLDVGHLPLCNENDSLQNGQVKTEESPPSNGGKTRRKKQGKKNETIDLRNLLLMCSQSVYANDNRNANELLKQIRQHSSPSGDGPQRLAHYFANGLEARLVGDGTGAQTFHSSPNTKRISTAEFLKAYQVHLSTSPFKKFAYFFANKMIMKASANAETLHIIDFGILYGFQWPILIKFLSDREGGPPNLKITGIEFPLPGFRPMERIEETGRRLADYCKRFHVPFEFNAIPSRYWETIRVEDLKIKSNEVVVVNSLMRFKNLLDESIEANSPRNAVLHLIRKINPAIFVQSIVNGSYNSPFFATRFREALFHFSALYDMFDTVIPRENKYRMLMERESIGREAMNVVACEGLERVERPETYKQWQVRNTRADFKQLPLNSELMDKFRTKLQQCYHKDFVFDVDNNWMLQGWKGRILYASTCWVPA