AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene071306

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071306.t1 MTR_8g464430 59.028 144 35 2 52 171 1 144 6.12e-44 142
MS.gene071306.t1 MTR_4g094430 51.240 121 45 2 57 164 169 288 9.18e-33 118
MS.gene071306.t1 MTR_2g032060 47.934 121 49 2 57 164 169 288 9.34e-30 110
MS.gene071306.t1 MTR_8g461270 41.667 144 67 3 33 163 196 335 1.44e-29 111
MS.gene071306.t1 MTR_4g007750 47.500 120 48 2 57 163 186 303 1.55e-28 107
MS.gene071306.t1 MTR_8g464440 88.462 52 6 0 1 52 24 75 1.06e-27 101
MS.gene071306.t1 MTR_1g075610 45.000 120 51 2 57 163 191 308 2.96e-26 101
MS.gene071306.t1 MTR_3g085650 39.062 128 58 3 57 164 581 708 2.26e-20 87.4
MS.gene071306.t1 MTR_4g103810 39.062 128 58 3 57 164 275 402 2.37e-20 87.0
MS.gene071306.t1 MTR_3g085650 39.062 128 58 3 57 164 581 708 2.57e-20 87.4
MS.gene071306.t1 MTR_4g103810 38.281 128 59 3 57 164 385 512 2.59e-20 87.4
MS.gene071306.t1 MTR_3g085650 39.062 128 58 3 57 164 581 708 3.46e-20 87.0
MS.gene071306.t1 MTR_4g061320 36.000 125 64 3 57 166 206 329 1.56e-19 84.3
MS.gene071306.t1 MTR_4g087620 36.719 128 64 5 57 170 221 345 2.60e-19 84.0
MS.gene071306.t1 MTR_8g098845 37.600 125 62 4 57 166 218 341 5.84e-19 82.8
MS.gene071306.t1 MTR_8g080190 37.500 128 60 3 57 164 550 677 8.34e-19 83.2
MS.gene071306.t1 MTR_7g078690 36.885 122 61 4 57 163 204 324 2.49e-18 81.3
MS.gene071306.t1 MTR_5g010030 36.154 130 67 4 57 171 207 335 1.56e-17 79.0
MS.gene071306.t1 MTR_3g460950 35.000 120 65 1 57 163 199 318 1.58e-17 79.0
MS.gene071306.t1 MTR_3g060330 35.000 120 65 1 57 163 199 318 1.58e-17 79.0
MS.gene071306.t1 MTR_6g080470 36.667 120 61 2 57 163 177 294 4.83e-17 77.8
MS.gene071306.t1 MTR_3g054080 35.000 120 63 2 57 163 179 296 5.04e-17 77.8
MS.gene071306.t1 MTR_8g028720 33.607 122 65 3 57 163 203 323 3.00e-16 75.1
MS.gene071306.t1 MTR_3g112210 37.398 123 59 5 57 163 199 319 3.89e-16 75.1
MS.gene071306.t1 MTR_6g087920 35.714 126 67 2 52 163 275 400 5.24e-16 74.7
MS.gene071306.t1 MTR_8g086000 33.607 122 65 3 57 163 199 319 7.18e-16 74.3
MS.gene071306.t1 MTR_8g086000 33.607 122 65 3 57 163 242 362 7.40e-16 74.3
MS.gene071306.t1 MTR_3g040810 38.017 121 60 3 57 163 203 322 1.04e-15 73.9
MS.gene071306.t1 MTR_3g096960 31.618 136 65 3 57 165 196 330 4.57e-15 72.0
MS.gene071306.t1 MTR_3g040520 35.833 120 64 1 57 163 205 324 5.17e-15 72.0
MS.gene071306.t1 MTR_3g085650 38.393 112 49 3 57 148 581 692 8.01e-15 71.6
MS.gene071306.t1 MTR_8g106960 37.405 131 63 6 57 170 288 416 8.62e-15 71.6
MS.gene071306.t1 MTR_8g106960 37.405 131 63 6 57 170 288 416 8.98e-15 71.2
MS.gene071306.t1 MTR_5g008860 33.058 121 65 3 57 163 306 424 5.88e-14 68.9
MS.gene071306.t1 MTR_7g110060 35.385 130 68 3 47 162 231 358 7.50e-14 68.6
MS.gene071306.t1 MTR_8g103240 32.540 126 71 2 52 163 288 413 9.74e-14 68.2
MS.gene071306.t1 MTR_4g117490 38.281 128 60 6 57 167 195 320 9.93e-14 68.6
MS.gene071306.t1 MTR_1g086370 34.351 131 70 3 47 163 112 240 1.04e-13 67.8
MS.gene071306.t1 MTR_1g086370 34.351 131 70 3 47 163 198 326 1.14e-13 68.2
MS.gene071306.t1 MTR_4g117490 38.710 124 57 6 57 163 195 316 1.46e-13 67.8
MS.gene071306.t1 MTR_1g086370 34.351 131 70 3 47 163 231 359 1.46e-13 67.8
MS.gene071306.t1 MTR_2g005620 35.115 131 69 3 47 163 246 374 1.76e-13 67.4
MS.gene071306.t1 MTR_2g021330 37.500 128 61 6 57 167 195 320 2.24e-13 67.4
MS.gene071306.t1 MTR_8g071230 30.579 121 71 1 57 164 164 284 2.78e-13 67.0
MS.gene071306.t1 MTR_2g021330 37.500 128 61 6 57 167 195 320 2.85e-13 67.0
MS.gene071306.t1 MTR_4g109090 31.967 122 67 3 57 164 271 390 3.02e-13 67.0
MS.gene071306.t1 MTR_1g084760 33.588 131 71 3 47 163 231 359 4.51e-13 66.2
MS.gene071306.t1 MTR_4g109090 31.967 122 67 3 57 164 123 242 6.79e-13 65.9
MS.gene071306.t1 MTR_3g460980 33.058 121 64 4 58 163 170 288 1.44e-12 64.7
MS.gene071306.t1 MTR_3g060390 33.058 121 64 4 58 163 166 284 1.46e-12 64.7
MS.gene071306.t1 MTR_5g026960 29.752 121 72 1 57 164 164 284 1.72e-12 64.7
MS.gene071306.t1 MTR_7g076030 33.588 131 71 3 47 163 190 318 3.38e-12 63.5
MS.gene071306.t1 MTR_2g083940 34.711 121 63 3 57 163 200 318 4.35e-12 63.5
MS.gene071306.t1 MTR_2g083940 34.711 121 63 3 57 163 254 372 4.52e-12 63.5
MS.gene071306.t1 MTR_4g061130 37.607 117 64 5 57 170 205 315 4.62e-12 63.2
MS.gene071306.t1 MTR_2g016490 35.537 121 62 4 57 163 206 324 6.31e-12 63.2
MS.gene071306.t1 MTR_7g076030 33.588 131 71 3 47 163 297 425 6.87e-12 63.2
MS.gene071306.t1 MTR_8g080190 43.939 66 24 1 57 109 550 615 7.01e-12 63.2
MS.gene071306.t1 MTR_5g091680 37.903 124 58 5 57 163 192 313 7.57e-12 62.8
MS.gene071306.t1 MTR_1g098300 33.607 122 61 3 57 163 292 408 7.99e-12 62.8
MS.gene071306.t1 MTR_5g091680 37.500 128 61 5 57 167 192 317 8.40e-12 62.8
MS.gene071306.t1 MTR_5g091680 37.903 124 58 5 57 163 190 311 8.40e-12 62.8
MS.gene071306.t1 MTR_5g091680 37.903 124 58 5 57 163 192 313 8.48e-12 62.8
MS.gene071306.t1 MTR_6g013030 31.496 127 65 4 57 166 276 397 9.05e-12 62.8
MS.gene071306.t1 MTR_2g020210 32.231 121 66 3 57 163 268 386 1.27e-11 62.4
MS.gene071306.t1 MTR_8g012450 28.800 125 75 2 57 167 164 288 1.28e-11 62.0
MS.gene071306.t1 MTR_8g012450 28.800 125 75 2 57 167 164 288 1.30e-11 62.0
MS.gene071306.t1 MTR_3g098760 33.058 121 65 4 57 163 263 381 2.83e-11 61.2
MS.gene071306.t1 MTR_7g011800 31.967 122 66 3 57 163 286 405 2.98e-11 61.2
MS.gene071306.t1 MTR_6g079470 37.405 131 63 6 57 170 259 387 3.22e-11 61.2
MS.gene071306.t1 MTR_8g432510 32.836 134 74 3 47 166 295 426 6.09e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene071306.t1 AT2G38620 47.500 120 48 2 57 163 124 241 1.85e-29 108
MS.gene071306.t1 AT2G38620 47.500 120 48 2 57 163 186 303 6.05e-29 108
MS.gene071306.t1 AT3G54180 46.667 120 49 2 57 163 184 301 3.72e-28 106
MS.gene071306.t1 AT3G48750 46.341 123 52 2 57 166 169 290 5.53e-28 105
MS.gene071306.t1 AT1G76540 46.667 120 49 2 57 163 187 304 3.84e-27 104
MS.gene071306.t1 AT1G20930 45.833 120 50 2 57 163 189 306 9.97e-27 103
MS.gene071306.t1 AT3G59790 38.843 121 59 3 57 163 226 345 1.05e-20 87.8
MS.gene071306.t1 AT1G18040 37.190 121 59 3 57 163 175 292 8.52e-20 85.5
MS.gene071306.t1 AT1G07880 36.885 122 61 3 57 163 199 319 1.97e-19 84.0
MS.gene071306.t1 AT1G67580 36.719 128 61 3 57 164 571 698 1.16e-18 82.8
MS.gene071306.t1 AT1G67580 36.719 128 61 3 57 164 571 698 1.16e-18 82.8
MS.gene071306.t1 AT1G73690 37.190 121 59 3 57 163 174 291 3.69e-18 80.9
MS.gene071306.t1 AT1G01560 36.066 122 62 3 57 163 206 326 7.36e-18 79.7
MS.gene071306.t1 AT4G11330 35.385 130 68 4 57 171 209 337 1.04e-17 79.3
MS.gene071306.t1 AT3G45640 36.923 130 66 4 57 171 204 332 1.25e-17 79.3
MS.gene071306.t1 AT5G63370 37.209 129 57 4 57 163 467 593 1.69e-17 79.3
MS.gene071306.t1 AT5G63370 37.209 129 57 4 57 163 467 593 1.69e-17 79.3
MS.gene071306.t1 AT5G63370 37.209 129 57 4 57 163 467 593 1.69e-17 79.3
MS.gene071306.t1 AT5G63370 37.209 129 57 4 57 163 467 593 1.69e-17 79.3
MS.gene071306.t1 AT5G63370 37.209 129 57 4 57 163 467 593 1.69e-17 79.3
MS.gene071306.t1 AT5G63370 37.209 129 57 4 57 163 333 459 2.64e-17 78.6
MS.gene071306.t1 AT5G63370 37.209 129 57 4 57 163 333 459 2.64e-17 78.6
MS.gene071306.t1 AT4G01370 36.885 122 61 4 57 163 209 329 4.42e-17 77.8
MS.gene071306.t1 AT2G43790 37.500 128 63 5 57 170 229 353 5.80e-17 77.4
MS.gene071306.t1 AT2G46070 38.211 123 58 5 57 163 207 327 6.50e-17 77.0
MS.gene071306.t1 AT2G46070 38.211 123 58 5 57 163 241 361 6.65e-17 77.4
MS.gene071306.t1 AT5G63610 34.559 136 61 3 57 165 201 335 1.20e-16 76.6
MS.gene071306.t1 AT1G59580 38.710 124 56 5 57 163 199 319 1.22e-16 76.3
MS.gene071306.t1 AT1G59580 38.710 124 56 5 57 163 199 319 1.22e-16 76.3
MS.gene071306.t1 AT5G10270 38.017 121 60 3 57 163 206 325 2.17e-16 76.3
MS.gene071306.t1 AT5G64960 34.167 120 66 1 57 163 206 325 5.93e-16 74.7
MS.gene071306.t1 AT3G01085 36.364 121 62 3 57 163 281 400 5.96e-16 74.7
MS.gene071306.t1 AT3G01085 36.364 121 62 3 57 163 281 400 5.96e-16 74.7
MS.gene071306.t1 AT3G01085 36.364 121 62 3 57 163 281 400 6.02e-16 74.7
MS.gene071306.t1 AT5G64960 34.167 120 66 1 57 163 153 272 6.28e-16 74.7
MS.gene071306.t1 AT1G10210 37.398 123 59 5 57 163 199 319 8.71e-16 73.9
MS.gene071306.t1 AT1G10210 37.398 123 59 5 57 163 199 319 8.71e-16 73.9
MS.gene071306.t1 AT1G10210 37.398 123 59 5 57 163 199 319 8.71e-16 73.9
MS.gene071306.t1 AT1G66750 35.000 120 63 2 57 163 176 293 8.98e-16 73.9
MS.gene071306.t1 AT4G19110 33.058 121 68 1 57 164 164 284 9.86e-15 71.2
MS.gene071306.t1 AT2G01450 38.168 131 62 5 57 170 186 314 1.03e-14 71.2
MS.gene071306.t1 AT2G01450 38.168 131 62 5 57 170 186 314 1.03e-14 71.2
MS.gene071306.t1 AT2G01450 38.168 131 62 5 57 170 186 314 1.03e-14 71.2
MS.gene071306.t1 AT2G01450 38.168 131 62 5 57 170 186 314 1.03e-14 71.2
MS.gene071306.t1 AT4G19110 33.058 121 68 1 57 164 164 284 1.19e-14 70.9
MS.gene071306.t1 AT4G19110 33.058 121 68 1 57 164 108 228 1.34e-14 70.9
MS.gene071306.t1 AT2G18170 30.464 151 71 5 35 163 181 319 2.42e-14 70.1
MS.gene071306.t1 AT4G13020 32.258 124 71 1 57 167 172 295 4.92e-14 69.3
MS.gene071306.t1 AT4G13020 32.258 124 71 1 57 167 172 295 4.92e-14 69.3
MS.gene071306.t1 AT4G13020 32.258 124 71 1 57 167 173 296 5.31e-14 68.9
MS.gene071306.t1 AT4G13020 32.258 124 71 1 57 167 164 287 5.47e-14 68.9
MS.gene071306.t1 AT4G13020 32.258 124 71 1 57 167 164 287 5.48e-14 68.9
MS.gene071306.t1 AT1G57870 35.878 131 68 3 47 163 238 366 5.57e-14 68.9
MS.gene071306.t1 AT4G13020 32.258 124 71 1 57 167 164 287 5.60e-14 68.9
MS.gene071306.t1 AT4G13020 32.258 124 71 1 57 167 173 296 5.90e-14 68.9
MS.gene071306.t1 AT4G13020 32.258 124 71 1 57 167 164 287 6.28e-14 68.9
MS.gene071306.t1 AT1G57870 35.878 131 68 3 47 163 238 366 7.02e-14 68.6
MS.gene071306.t1 AT1G57870 35.878 131 68 3 47 163 238 366 7.02e-14 68.6
MS.gene071306.t1 AT1G57870 35.878 131 68 3 47 163 238 366 7.02e-14 68.6
MS.gene071306.t1 AT4G36450 32.540 126 61 3 57 163 196 316 8.68e-14 68.2
MS.gene071306.t1 AT1G73670 38.636 132 56 7 57 168 260 386 1.27e-13 68.2
MS.gene071306.t1 AT4G28980 34.127 126 69 2 52 163 293 418 1.64e-13 67.8
MS.gene071306.t1 AT4G28980 34.127 126 69 2 52 163 293 418 1.64e-13 67.8
MS.gene071306.t1 AT4G28980 34.127 126 69 2 52 163 293 418 1.64e-13 67.8
MS.gene071306.t1 AT3G05840 33.588 131 71 3 47 163 229 357 1.64e-13 67.8
MS.gene071306.t1 AT3G05840 33.588 131 71 3 47 163 229 357 1.64e-13 67.8
MS.gene071306.t1 AT5G19010 38.281 128 60 6 57 167 195 320 1.77e-13 67.8
MS.gene071306.t1 AT5G39420 33.058 121 65 3 57 163 271 389 1.78e-13 67.8
MS.gene071306.t1 AT1G01560 33.613 119 61 4 57 158 206 323 2.14e-13 67.0
MS.gene071306.t1 AT5G14640 34.351 131 70 3 47 163 230 358 3.27e-13 66.6
MS.gene071306.t1 AT5G14640 34.351 131 70 3 47 163 230 358 3.27e-13 66.6
MS.gene071306.t1 AT5G26751 33.588 131 71 3 47 163 225 353 3.86e-13 66.6
MS.gene071306.t1 AT1G09840 35.659 129 67 3 47 161 112 238 5.22e-13 65.9
MS.gene071306.t1 AT1G09840 35.659 129 67 3 47 161 239 365 8.52e-13 65.5
MS.gene071306.t1 AT1G09840 35.659 129 67 3 47 161 239 365 8.52e-13 65.5
MS.gene071306.t1 AT1G09840 35.659 129 67 3 47 161 239 365 8.52e-13 65.5
MS.gene071306.t1 AT1G09840 35.659 129 67 3 47 161 239 365 8.52e-13 65.5
MS.gene071306.t1 AT1G09840 35.659 129 67 3 47 161 239 365 8.52e-13 65.5
MS.gene071306.t1 AT1G09840 35.659 129 67 3 47 161 239 365 8.52e-13 65.5
MS.gene071306.t1 AT1G09840 35.659 129 67 3 47 161 239 365 8.52e-13 65.5
MS.gene071306.t1 AT1G09840 35.659 129 67 3 47 161 239 365 8.52e-13 65.5
MS.gene071306.t1 AT1G09840 35.659 129 67 3 47 161 239 365 8.52e-13 65.5
MS.gene071306.t1 AT1G09840 35.659 129 67 3 47 161 239 365 8.52e-13 65.5
MS.gene071306.t1 AT4G00720 33.588 131 71 3 47 163 294 422 2.18e-12 64.3
MS.gene071306.t1 AT2G46070 35.398 113 55 5 57 153 207 317 3.01e-12 63.9
MS.gene071306.t1 AT1G53050 34.711 121 63 3 57 163 300 418 3.59e-12 63.9
MS.gene071306.t1 AT1G53050 34.711 121 63 3 57 163 300 418 3.59e-12 63.9
MS.gene071306.t1 AT5G45430 30.579 121 71 1 57 164 164 284 5.39e-12 63.5
MS.gene071306.t1 AT5G45430 30.579 121 71 1 57 164 164 284 6.28e-12 63.2
MS.gene071306.t1 AT1G54610 29.167 120 71 2 57 163 284 402 6.88e-12 63.2
MS.gene071306.t1 AT1G54610 29.167 120 71 2 57 163 284 402 6.88e-12 63.2
MS.gene071306.t1 AT1G54610 29.167 120 71 2 57 163 284 402 6.88e-12 63.2
MS.gene071306.t1 AT1G09600 33.058 121 65 4 57 163 329 447 7.19e-12 63.2
MS.gene071306.t1 AT2G38620 49.123 57 24 1 57 113 186 237 8.23e-12 62.0
MS.gene071306.t1 AT2G38620 49.123 57 24 1 57 113 186 237 8.23e-12 62.0
MS.gene071306.t1 AT2G42880 40.310 129 54 7 57 166 195 319 1.15e-11 62.4
MS.gene071306.t1 AT2G42880 40.310 129 54 7 57 166 195 319 1.46e-11 62.0
MS.gene071306.t1 AT3G61160 30.534 131 75 3 47 163 258 386 1.55e-11 62.0
MS.gene071306.t1 AT3G61160 30.534 131 75 3 47 163 258 386 1.70e-11 62.0
MS.gene071306.t1 AT3G61160 30.534 131 75 3 47 163 265 393 1.83e-11 61.6
MS.gene071306.t1 AT1G06390 32.061 131 73 3 47 163 226 354 1.94e-11 61.6
MS.gene071306.t1 AT1G06390 32.061 131 73 3 47 163 226 354 1.94e-11 61.6
MS.gene071306.t1 AT5G63370 42.466 73 29 1 57 116 467 539 2.32e-11 61.6
MS.gene071306.t1 AT5G63370 42.466 73 29 1 57 116 467 539 2.32e-11 61.6
MS.gene071306.t1 AT5G63370 42.466 73 29 1 57 116 333 405 2.61e-11 61.2
MS.gene071306.t1 AT5G44290 32.787 122 66 3 57 164 303 422 3.12e-11 61.2
MS.gene071306.t1 AT5G44290 32.787 122 66 3 57 164 303 422 3.37e-11 61.2
MS.gene071306.t1 AT5G44290 32.787 122 66 3 57 164 303 422 3.95e-11 60.8
MS.gene071306.t1 AT5G44290 32.787 122 66 3 57 164 303 422 3.95e-11 60.8
MS.gene071306.t1 AT5G44290 32.787 122 66 3 57 164 303 422 3.95e-11 60.8
MS.gene071306.t1 AT5G44290 32.787 122 66 3 57 164 303 422 3.95e-11 60.8
MS.gene071306.t1 AT5G44290 32.787 122 66 3 57 164 303 422 3.95e-11 60.8
MS.gene071306.t1 AT2G30980 33.058 121 65 3 57 163 238 356 4.41e-11 60.8
MS.gene071306.t1 AT1G03740 30.400 125 65 4 57 164 379 498 9.56e-11 59.7

Find 45 sgRNAs with CRISPR-Local

Find 85 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAGCTGAAATCCTGCTATT+TGG 0.134409 8.2:-45882435 None:intergenic
TGCAGTTGATATCTGGTCTA+TGG 0.259046 8.2:+45881808 MS.gene071306:CDS
AAGATTGACCCGACAATATT+GGG 0.286282 8.2:-45880919 None:intergenic
ATCAACTGCAGTTGAGTAAT+TGG 0.289792 8.2:-45881795 None:intergenic
AAAGATTGACCCGACAATAT+TGG 0.297856 8.2:-45880920 None:intergenic
ATGAATACCTTTGGAACAAT+TGG 0.311260 8.2:-45882073 None:intergenic
CAGCTGAAATCCTGCTATTT+GGG 0.318029 8.2:-45882434 None:intergenic
CTGCTATCTATTCAGATTGC+AGG 0.356258 8.2:+45881096 MS.gene071306:intron
TAAAGCACCTGAACCTTTGA+TGG 0.361768 8.2:+45881766 MS.gene071306:CDS
ATATGGTCTTGATCTTCTTT+CGG 0.371973 8.2:+45882207 MS.gene071306:CDS
ACTCAACTGCAGTTGATATC+TGG 0.396574 8.2:+45881801 MS.gene071306:CDS
AGACCATATGGTTCGAGCTC+AGG 0.416059 8.2:-45882193 None:intergenic
GCATATTTGCTGAGATGCTT+AGG 0.419785 8.2:+45881834 MS.gene071306:CDS
GTAGAACCCATCAAAGGTTC+AGG 0.434339 8.2:-45881773 None:intergenic
GTTCATCATTACCAATGAAC+AGG 0.435723 8.2:-45881863 None:intergenic
GTGGTTAAAATTCGAAGATA+TGG 0.441943 8.2:-45882474 None:intergenic
CGAGGCCAGGTTTCTTCAGT+TGG 0.445405 8.2:-45882010 None:intergenic
TTAGGCTCGAACCTGTTCAT+TGG 0.452136 8.2:+45881852 MS.gene071306:CDS
TCGAAAACACGTCGCCAATC+CGG 0.480236 8.2:+45881177 MS.gene071306:CDS
GACCATATGGTTCGAGCTCA+GGG 0.480813 8.2:-45882192 None:intergenic
GAAAGAAGATCAAGACCATA+TGG 0.490142 8.2:-45882205 None:intergenic
TTTGCCTGTGCCCAAATAGC+AGG 0.491915 8.2:+45882424 MS.gene071306:CDS
TTCCCTGAGCTCGAACCATA+TGG 0.494924 8.2:+45882190 MS.gene071306:CDS
AATACCTTTGGAACAATTGG+AGG 0.500345 8.2:-45882070 None:intergenic
TTGGACCTCCCCAATATTGT+CGG 0.500470 8.2:+45880910 MS.gene071306:CDS
ATTCAGATTGCAGGCAGTGA+TGG 0.510492 8.2:+45881105 MS.gene071306:intron
AGGAGCTTCATCAATCATCA+TGG 0.514486 8.2:-45882497 None:intergenic
CAACCCTCCAATTGTTCCAA+AGG 0.531079 8.2:+45882066 MS.gene071306:CDS
GCAGTTGATATCTGGTCTAT+GGG 0.534989 8.2:+45881809 MS.gene071306:CDS
AAATCCTGCTATTTGGGCAC+AGG 0.541827 8.2:-45882428 None:intergenic
TATAGAAGACACACGAGGCC+AGG 0.545839 8.2:-45882023 None:intergenic
GGTTCAGGTGCTTTATAGCA+CGG 0.550189 8.2:-45881758 None:intergenic
TGGACCTCCCCAATATTGTC+GGG 0.558796 8.2:+45880911 MS.gene071306:CDS
AAAGCACCTGAACCTTTGAT+GGG 0.566858 8.2:+45881767 MS.gene071306:CDS
GCTCAGGGAATTCTGCTGCT+AGG 0.577996 8.2:-45882177 None:intergenic
GTACGCCAACTGAAGAAACC+TGG 0.610822 8.2:+45882005 MS.gene071306:CDS
GGAGCTTCATCAATCATCAT+GGG 0.611673 8.2:-45882496 None:intergenic
ATACCTTTGGAACAATTGGA+GGG 0.612490 8.2:-45882069 None:intergenic
GCAGTGATGGAAACTGAGGA+TGG 0.629963 8.2:+45881118 MS.gene071306:CDS
AGATTGACCCGACAATATTG+GGG 0.646668 8.2:-45880918 None:intergenic
TAATTGGTAGAACCCATCAA+AGG 0.667309 8.2:-45881779 None:intergenic
GCTTCATCAATCATCATGGG+TGG 0.679817 8.2:-45882493 None:intergenic
TTGACCCGACAATATTGGGG+AGG 0.695566 8.2:-45880915 None:intergenic
GCAGGCAGTGATGGAAACTG+AGG 0.718238 8.2:+45881114 MS.gene071306:CDS
TTGCATATAGAAGACACACG+AGG 0.817903 8.2:-45882028 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATATTGCTTATTTATAAA+AGG - chr8.2:45882131-45882150 None:intergenic 10.0%
!!! ATTAATAACTTCATTTTTTT+TGG - chr8.2:45881700-45881719 None:intergenic 10.0%
!!! TTAATAACTTCATTTTTTTT+GGG - chr8.2:45881699-45881718 None:intergenic 10.0%
!! AATAGAAACACAATAAATTA+TGG - chr8.2:45881320-45881339 None:intergenic 15.0%
!! AGAAATCATAAAAATAGTTA+TGG - chr8.2:45881931-45881950 None:intergenic 15.0%
!! ATTTATTCATTAATAGAGTA+GGG - chr8.2:45881670-45881689 None:intergenic 15.0%
!!! ATTTATTTTCAATTTTCAGA+TGG + chr8.2:45881730-45881749 MS.gene071306:intron 15.0%
!!! TTTATTTTCAATTTTCAGAT+GGG + chr8.2:45881731-45881750 MS.gene071306:intron 15.0%
!! AATTAATTCTCAAAGATATG+AGG - chr8.2:45882244-45882263 None:intergenic 20.0%
!! ATCTAGTGAATGTTTATTAA+TGG + chr8.2:45882373-45882392 MS.gene071306:intron 20.0%
!! CAATATTATATGCAAACTAT+TGG + chr8.2:45882143-45882162 MS.gene071306:intron 20.0%
!! CATTTATTCATTAATAGAGT+AGG - chr8.2:45881671-45881690 None:intergenic 20.0%
!! TGCTTATTTATAAAAGGTAA+TGG - chr8.2:45882125-45882144 None:intergenic 20.0%
!! TTATATATTAGATGCAAACA+AGG + chr8.2:45881439-45881458 MS.gene071306:intron 20.0%
!! TTTAAGCAGATTGAAAATAT+AGG + chr8.2:45882332-45882351 MS.gene071306:intron 20.0%
!!! ATCTGAAATCTAATATATTC+TGG + chr8.2:45881563-45881582 MS.gene071306:intron 20.0%
! ATAAACATTCACTAGATCAA+TGG - chr8.2:45882370-45882389 None:intergenic 25.0%
! ATATCTTATAGTGCAAGAAA+AGG + chr8.2:45881342-45881361 MS.gene071306:intron 25.0%
! CAAAATCAATAAATGTGACA+AGG - chr8.2:45880996-45881015 None:intergenic 25.0%
!! AGATATTGGATGCAATATTT+TGG + chr8.2:45881889-45881908 MS.gene071306:intron 25.0%
!!! TATCTTTGTTTTCAACATCA+TGG + chr8.2:45881292-45881311 MS.gene071306:intron 25.0%
!!! TTTTATATGTAAAGCTTGCT+TGG + chr8.2:45881983-45882002 MS.gene071306:intron 25.0%
AAGTAGAACATGAATACCTT+TGG - chr8.2:45882085-45882104 None:intergenic 30.0%
AATATTGCATCCAATATCTG+CGG - chr8.2:45881888-45881907 None:intergenic 30.0%
CATATTCGCAAAATTCTACA+CGG + chr8.2:45881538-45881557 MS.gene071306:intron 30.0%
GTGGTTAAAATTCGAAGATA+TGG - chr8.2:45882477-45882496 None:intergenic 30.0%
! ATATGGTCTTGATCTTCTTT+CGG + chr8.2:45882207-45882226 MS.gene071306:CDS 30.0%
! GCAAACTATTGGATTTTGTA+GGG + chr8.2:45882154-45882173 MS.gene071306:intron 30.0%
! TGCAAACTATTGGATTTTGT+AGG + chr8.2:45882153-45882172 MS.gene071306:intron 30.0%
! TTTGGAATGCTTAAAAACTC+CGG - chr8.2:45881199-45881218 None:intergenic 30.0%
!! AGTTTTTAAGCATTCCAAAC+TGG + chr8.2:45881200-45881219 MS.gene071306:CDS 30.0%
!! ATGAATACCTTTGGAACAAT+TGG - chr8.2:45882076-45882095 None:intergenic 30.0%
!!! TGACTTACTTTTTTCCAGTT+TGG - chr8.2:45881217-45881236 None:intergenic 30.0%
AAAGATTGACCCGACAATAT+TGG - chr8.2:45880923-45880942 None:intergenic 35.0%
AAGATTGACCCGACAATATT+GGG - chr8.2:45880922-45880941 None:intergenic 35.0%
AAGCTATCTTGACTTTAGTG+CGG + chr8.2:45881618-45881637 MS.gene071306:intron 35.0%
AATGCTTAAAAACTCCGGAT+TGG - chr8.2:45881194-45881213 None:intergenic 35.0%
ATCAACTGCAGTTGAGTAAT+TGG - chr8.2:45881798-45881817 None:intergenic 35.0%
GAAAGAAGATCAAGACCATA+TGG - chr8.2:45882208-45882227 None:intergenic 35.0%
GTTCATCATTACCAATGAAC+AGG - chr8.2:45881866-45881885 None:intergenic 35.0%
TAATGATGAACCGCAGATAT+TGG + chr8.2:45881875-45881894 MS.gene071306:intron 35.0%
TAATTGGTAGAACCCATCAA+AGG - chr8.2:45881782-45881801 None:intergenic 35.0%
TTAATAGAGTAGGGTGAAAG+AGG - chr8.2:45881661-45881680 None:intergenic 35.0%
! CGTGTAGAATTTTGCGAATA+TGG - chr8.2:45881540-45881559 None:intergenic 35.0%
! TAATGGCAAGTGTTTTGTGT+TGG + chr8.2:45882390-45882409 MS.gene071306:intron 35.0%
! TCTGGCTTCAGTTGAAAAAT+TGG + chr8.2:45881581-45881600 MS.gene071306:intron 35.0%
!! AATACCTTTGGAACAATTGG+AGG - chr8.2:45882073-45882092 None:intergenic 35.0%
!! ATACCTTTGGAACAATTGGA+GGG - chr8.2:45882072-45882091 None:intergenic 35.0%
AGATTGACCCGACAATATTG+GGG - chr8.2:45880921-45880940 None:intergenic 40.0%
CTGCTATCTATTCAGATTGC+AGG + chr8.2:45881096-45881115 MS.gene071306:intron 40.0%
GAAAAATCTGCAAGGACAAG+AGG - chr8.2:45881487-45881506 None:intergenic 40.0%
TCAGCTGAAATCCTGCTATT+TGG - chr8.2:45882438-45882457 None:intergenic 40.0%
TTCACGCAGAAAAATCTGCA+AGG - chr8.2:45881495-45881514 None:intergenic 40.0%
TTGCATATAGAAGACACACG+AGG - chr8.2:45882031-45882050 None:intergenic 40.0%
! AAAGCACCTGAACCTTTGAT+GGG + chr8.2:45881767-45881786 MS.gene071306:CDS 40.0%
! ACTCAACTGCAGTTGATATC+TGG + chr8.2:45881801-45881820 MS.gene071306:CDS 40.0%
! CAGCTGAAATCCTGCTATTT+GGG - chr8.2:45882437-45882456 None:intergenic 40.0%
! GCAGTTGATATCTGGTCTAT+GGG + chr8.2:45881809-45881828 MS.gene071306:CDS 40.0%
! GCATATTTGCTGAGATGCTT+AGG + chr8.2:45881834-45881853 MS.gene071306:CDS 40.0%
! TAAAGCACCTGAACCTTTGA+TGG + chr8.2:45881766-45881785 MS.gene071306:CDS 40.0%
! TGCAGTTGATATCTGGTCTA+TGG + chr8.2:45881808-45881827 MS.gene071306:CDS 40.0%
ATAGAGTAGGGTGAAAGAGG+AGG - chr8.2:45881658-45881677 None:intergenic 45.0%
ATTCAGATTGCAGGCAGTGA+TGG + chr8.2:45881105-45881124 MS.gene071306:intron 45.0%
CAACCCTCCAATTGTTCCAA+AGG + chr8.2:45882066-45882085 MS.gene071306:CDS 45.0%
CTGCAAGGACAAGAGGAAAA+CGG - chr8.2:45881480-45881499 None:intergenic 45.0%
GGTTCAGGTGCTTTATAGCA+CGG - chr8.2:45881761-45881780 None:intergenic 45.0%
GTAGAACCCATCAAAGGTTC+AGG - chr8.2:45881776-45881795 None:intergenic 45.0%
TTAGGCTCGAACCTGTTCAT+TGG + chr8.2:45881852-45881871 MS.gene071306:CDS 45.0%
TTGGACCTCCCCAATATTGT+CGG + chr8.2:45880910-45880929 MS.gene071306:CDS 45.0%
! AAATCCTGCTATTTGGGCAC+AGG - chr8.2:45882431-45882450 None:intergenic 45.0%
AGACCATATGGTTCGAGCTC+AGG - chr8.2:45882196-45882215 None:intergenic 50.0%
GACCATATGGTTCGAGCTCA+GGG - chr8.2:45882195-45882214 None:intergenic 50.0%
GTACGCCAACTGAAGAAACC+TGG + chr8.2:45882005-45882024 MS.gene071306:CDS 50.0%
TATAGAAGACACACGAGGCC+AGG - chr8.2:45882026-45882045 None:intergenic 50.0%
TCGAAAACACGTCGCCAATC+CGG + chr8.2:45881177-45881196 MS.gene071306:CDS 50.0%
TGGACCTCCCCAATATTGTC+GGG + chr8.2:45880911-45880930 MS.gene071306:CDS 50.0%
TTCCCTGAGCTCGAACCATA+TGG + chr8.2:45882190-45882209 MS.gene071306:CDS 50.0%
TTGACCCGACAATATTGGGG+AGG - chr8.2:45880918-45880937 None:intergenic 50.0%
TTTCTGCGTGAAGCTCTCTC+TGG + chr8.2:45881503-45881522 MS.gene071306:intron 50.0%
TTTGCCTGTGCCCAAATAGC+AGG + chr8.2:45882424-45882443 MS.gene071306:CDS 50.0%
! GCAGTGATGGAAACTGAGGA+TGG + chr8.2:45881118-45881137 MS.gene071306:CDS 50.0%
CGAGGCCAGGTTTCTTCAGT+TGG - chr8.2:45882013-45882032 None:intergenic 55.0%
GCGTGAAGCTCTCTCTGGAT+TGG + chr8.2:45881508-45881527 MS.gene071306:intron 55.0%
GCTCAGGGAATTCTGCTGCT+AGG - chr8.2:45882180-45882199 None:intergenic 55.0%
! GCAGGCAGTGATGGAAACTG+AGG + chr8.2:45881114-45881133 MS.gene071306:CDS 55.0%


Chromosome Type Strat End Strand Name
chr8.2 gene 45880898 45882509 45880898 ID=MS.gene071306
chr8.2 mRNA 45880898 45882509 45880898 ID=MS.gene071306.t1;Parent=MS.gene071306
chr8.2 exon 45880898 45880932 45880898 ID=MS.gene071306.t1.exon1;Parent=MS.gene071306.t1
chr8.2 CDS 45880898 45880932 45880898 ID=cds.MS.gene071306.t1;Parent=MS.gene071306.t1
chr8.2 exon 45881111 45881228 45881111 ID=MS.gene071306.t1.exon2;Parent=MS.gene071306.t1
chr8.2 CDS 45881111 45881228 45881111 ID=cds.MS.gene071306.t1;Parent=MS.gene071306.t1
chr8.2 exon 45881749 45881873 45881749 ID=MS.gene071306.t1.exon3;Parent=MS.gene071306.t1
chr8.2 CDS 45881749 45881873 45881749 ID=cds.MS.gene071306.t1;Parent=MS.gene071306.t1
chr8.2 exon 45881997 45882087 45881997 ID=MS.gene071306.t1.exon4;Parent=MS.gene071306.t1
chr8.2 CDS 45881997 45882087 45881997 ID=cds.MS.gene071306.t1;Parent=MS.gene071306.t1
chr8.2 exon 45882175 45882228 45882175 ID=MS.gene071306.t1.exon5;Parent=MS.gene071306.t1
chr8.2 CDS 45882175 45882228 45882175 ID=cds.MS.gene071306.t1;Parent=MS.gene071306.t1
chr8.2 exon 45882417 45882509 45882417 ID=MS.gene071306.t1.exon6;Parent=MS.gene071306.t1
chr8.2 CDS 45882417 45882509 45882417 ID=cds.MS.gene071306.t1;Parent=MS.gene071306.t1
Gene Sequence

>MS.gene071306

ATGCTCAAAAACTTGGACCTCCCCAATATTGTCGGGTCAATCTTTTTCTGTTACAGTTAGCTTCTTTGTAGATATTAATTGATATATCATTCATGCCTTGTCACATTTATTGATTTTGTGATTTTAGTATAAGATGCATATATATATTCATTATTTAATGATGTTGATTCATTCACAGAAGTGATTTATATGTGTTTTCTGCTATCTATTCAGATTGCAGGCAGTGATGGAAACTGAGGATGGAGTGTTTCTTGTTTATGAGCATTTTCAAACTAGTCTTCGAAAACACGTCGCCAATCCGGAGTTTTTAAGCATTCCAAACTGGAAAAAAGTAAGTCAACTTTTGTTAACAATTCTGTGTAGAAATCTGTGTGATTCTAATCAAACTTGCATATATCTTTGTTTTCAACATCATGGTTCCATAATTTATTGTGTTTCTATTAGATATCTTATAGTGCAAGAAAAGGAAGAAGAATTTATGAAACAATTATATATCTTATTTCAATTATAGTTTTCACTTTTGTTTGAAGTTTGATAATTATTATATATTAGATGCAAACAAGGTTGCTATAATATAATCCGTTTTCCTCTTGTCCTTGCAGATTTTTCTGCGTGAAGCTCTCTCTGGATTGGCTTCTGCCATATTCGCAAAATTCTACACGGAGATCTGAAATCTAATATATTCTGGCTTCAGTTGAAAAATTGGATGTCAAGAAAGTGAAGCTATCTTGACTTTAGTGCGGCAAAAACTATTGAACCTCCTCTTTCACCCTACTCTATTAATGAATAAATGTTACTCCCAAAAAAAATGAAGTTATTAATGCTTTTGCAAATTTATTTTCAATTTTCAGATGGGTAATCCGTGCTATAAAGCACCTGAACCTTTGATGGGTTCTACCAATTACTCAACTGCAGTTGATATCTGGTCTATGGGATGCATATTTGCTGAGATGCTTAGGCTCGAACCTGTTCATTGGTAATGATGAACCGCAGATATTGGATGCAATATTTTGGTAATTTCATCAAAACACCATAACTATTTTTATGATTTCTCTGTACTTATATGAAATTAATGAGTTGAACATTTTTATATGTAAAGCTTGCTTGGTACGCCAACTGAAGAAACCTGGCCTCGTGTGTCTTCTATATGCAACTATATTGAAGCATTCAACCCTCCAATTGTTCCAAAGGTATTCATGTTCTACTTTTGATCAATTTTTTGTTCCATTACCTTTTATAAATAAGCAATATTATATGCAAACTATTGGATTTTGTAGGGCCTAGCAGCAGAATTCCCTGAGCTCGAACCATATGGTCTTGATCTTCTTTCGGTAAATTAACTGCCTCATATCTTTGAGAATTAATTCGCTTGTAAAACTTTTAGAATTTATTTATCATGCACTCACATCGCAAAATATTTTTCACAATAGCTATTTTAAGCAGATTGAAAATATAGGTAGTTAACTCTTCCATTGATCTAGTGAATGTTTATTAATGGCAAGTGTTTTGTGTTGGACAGAATATGCTTTGCCTGTGCCCAAATAGCAGGATTTCAGCTGATGAAGCTCTTGAGCATCCATATCTTCGAATTTTAACCACCCATGATGATTGA
Protein sequence

>MS.gene071306.t1

MLKNLDLPNIVGLQAVMETEDGVFLVYEHFQTSLRKHVANPEFLSIPNWKKMGNPCYKAPEPLMGSTNYSTAVDIWSMGCIFAEMLRLEPVHCLLGTPTEETWPRVSSICNYIEAFNPPIVPKGLAAEFPELEPYGLDLLSNMLCLCPNSRISADEALEHPYLRILTTHDD