AlfalfaGEDB | MS.gene071681

M. sativa cultivar XinJiangDaYe / MS.gene071681

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071681.t1	RHN69906.1	95.9	73	3	0	1	73	5	77	7.90E-36	159.1

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071681.t1	B9RU15	69.7	66	20	0	1	66	44	109	4.1e-25	114.8

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071681.t1	G7JB04	95.9	73	3	0	1	73	45	117	5.7e-36	159.1

TFs/TRs:

Gene ID	Type	Classification
MS.gene071681.t1	TR	PHD

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071681.t1	MTR_3g095830	95.890	73	3	0	1	73	45	117	6.77e-48	147
MS.gene071681.t1	MTR_3g095840	77.049	61	14	0	3	63	60	120	2.17e-31	112
MS.gene071681.t1	MTR_1g007670	54.167	48	22	0	16	63	26	73	4.25e-16	70.9
MS.gene071681.t1	MTR_5g010300	49.091	55	28	0	11	65	237	291	5.52e-14	65.1
MS.gene071681.t1	MTR_5g010300	49.091	55	28	0	11	65	237	291	5.91e-14	65.1
MS.gene071681.t1	MTR_5g010300	49.091	55	28	0	11	65	237	291	6.22e-14	65.1
MS.gene071681.t1	MTR_5g010300	49.091	55	28	0	11	65	237	291	6.34e-14	64.7
MS.gene071681.t1	MTR_1g007700	50.000	48	20	1	16	63	258	301	8.01e-13	61.6

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene071681.t1	AT5G09790	66.038	53	18	14	66	62	114	7.09e-22	87.0
MS.gene071681.t1	AT5G09790	66.038	53	18	14	66	62	114	7.33e-22	86.7
MS.gene071681.t1	AT5G24330	66.667	48	16	17	64	33	80	8.20e-19	78.2
MS.gene071681.t1	AT1G63490	43.750	64	36	2	65	225	288	8.22e-13	61.6
MS.gene071681.t1	AT1G63490	43.750	64	36	2	65	225	288	8.38e-13	61.6

Find 20 sgRNAs with CRISPR-Local

Find 18 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTAGAGTTCCTATTGGTTCA+TGG	0.294500	3.1:+72696947	MS.gene071681:CDS
GAAGTGTCTGAGACCGGTTC+TGG	0.362102	3.1:+72696924	MS.gene071681:CDS
CCCCAAGTGTTGTTCTGAGC+TGG	0.393884	3.1:+72696975	MS.gene071681:CDS
GGGCAAATCCATGAACCAAT+AGG	0.417784	3.1:-72696955	None:intergenic
CTCCAGCTCAGAACAACACT+TGG	0.460665	3.1:-72696977	None:intergenic
GTTCTGGCTAGAGTTCCTAT+TGG	0.469825	3.1:+72696940	MS.gene071681:CDS
ATGTGTGAGCGATGTGGATC+TGG	0.479905	3.1:+72696847	MS.gene071681:CDS
ATAGGAACTCTAGCCAGAAC+CGG	0.497595	3.1:-72696937	None:intergenic
TCACACAACAAAAGCTCCTC+AGG	0.503103	3.1:-72696877	None:intergenic
TCATATGAAGTGTCTGAGAC+CGG	0.525057	3.1:+72696918	MS.gene071681:CDS
TCCAGCTCAGAACAACACTT+GGG	0.544872	3.1:-72696976	None:intergenic
ATATGCAGTGGTTGAGAAGG+AGG	0.563550	3.1:+72696810	MS.gene071681:CDS
TAAATATGCAGTGGTTGAGA+AGG	0.574113	3.1:+72696807	MS.gene071681:CDS
TTGTGTGACAAATGCGACAA+AGG	0.628248	3.1:+72696892	MS.gene071681:CDS
CGATGACTAATATAATGGCG+AGG	0.630890	3.1:+72696782	None:intergenic
GATGACTAATATAATGGCGA+GGG	0.645664	3.1:+72696783	None:intergenic
GGCGAGGGCTAAATATGCAG+TGG	0.659115	3.1:+72696798	MS.gene071681:CDS
CCAGCTCAGAACAACACTTG+GGG	0.668343	3.1:-72696975	None:intergenic
GACTGCATGTGTGAGCGATG+TGG	0.685024	3.1:+72696841	MS.gene071681:CDS
TGGATCTGGTGAACAACCTG+AGG	0.697540	3.1:+72696861	MS.gene071681:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	TAAATATGCAGTGGTTGAGA+AGG	+	chr3.1:72696807-72696826	MS.gene071681:CDS	35.0%
	TTGTGTGACAAATGCGACAA+AGG	+	chr3.1:72696892-72696911	MS.gene071681:CDS	40.0%
!	TCATATGAAGTGTCTGAGAC+CGG	+	chr3.1:72696918-72696937	MS.gene071681:CDS	40.0%
!!	CTAGAGTTCCTATTGGTTCA+TGG	+	chr3.1:72696947-72696966	MS.gene071681:CDS	40.0%
	ATAGGAACTCTAGCCAGAAC+CGG	-	chr3.1:72696940-72696959	None:intergenic	45.0%
	ATATGCAGTGGTTGAGAAGG+AGG	+	chr3.1:72696810-72696829	MS.gene071681:CDS	45.0%
	GGGCAAATCCATGAACCAAT+AGG	-	chr3.1:72696958-72696977	None:intergenic	45.0%
	TCACACAACAAAAGCTCCTC+AGG	-	chr3.1:72696880-72696899	None:intergenic	45.0%
	TCCAGCTCAGAACAACACTT+GGG	-	chr3.1:72696979-72696998	None:intergenic	45.0%
!	GTTCTGGCTAGAGTTCCTAT+TGG	+	chr3.1:72696940-72696959	MS.gene071681:CDS	45.0%
	ATGTGTGAGCGATGTGGATC+TGG	+	chr3.1:72696847-72696866	MS.gene071681:CDS	50.0%
	CCAGCTCAGAACAACACTTG+GGG	-	chr3.1:72696978-72696997	None:intergenic	50.0%
	CTCCAGCTCAGAACAACACT+TGG	-	chr3.1:72696980-72696999	None:intergenic	50.0%
	TGGATCTGGTGAACAACCTG+AGG	+	chr3.1:72696861-72696880	MS.gene071681:CDS	50.0%
	GACTGCATGTGTGAGCGATG+TGG	+	chr3.1:72696841-72696860	MS.gene071681:CDS	55.0%
	GGCGAGGGCTAAATATGCAG+TGG	+	chr3.1:72696798-72696817	MS.gene071681:CDS	55.0%
!	CCCCAAGTGTTGTTCTGAGC+TGG	+	chr3.1:72696975-72696994	MS.gene071681:CDS	55.0%
!	GAAGTGTCTGAGACCGGTTC+TGG	+	chr3.1:72696924-72696943	MS.gene071681:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.1	gene	72696796	72697017	72696796	ID=MS.gene071681
chr3.1	mRNA	72696796	72697017	72696796	ID=MS.gene071681.t1;Parent=MS.gene071681
chr3.1	exon	72696796	72697017	72696796	ID=MS.gene071681.t1.exon1;Parent=MS.gene071681.t1
chr3.1	CDS	72696796	72697017	72696796	ID=cds.MS.gene071681.t1;Parent=MS.gene071681.t1

Gene Sequence	>MS.gene071681 ATGGCGAGGGCTAAATATGCAGTGGTTGAGAAGGAGGATTACAGTGACTGCATGTGTGAGCGATGTGGATCTGGTGAACAACCTGAGGAGCTTTTGTTGTGTGACAAATGCGACAAAGGTTTTCATATGAAGTGTCTGAGACCGGTTCTGGCTAGAGTTCCTATTGGTTCATGGATTTGCCCCAAGTGTTGTTCTGAGCTGGAGAGAGTGAAAAGTAAGTGA
Protein sequence	>MS.gene071681.t1 MARAKYAVVEKEDYSDCMCERCGSGEQPEELLLCDKCDKGFHMKCLRPVLARVPIGSWICPKCCSELERVKSK