Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071730.t1 | RHN42358.1 | 98.9 | 93 | 1 | 0 | 1 | 93 | 1 | 93 | 5.60E-43 | 183.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071730.t1 | Q39266 | 48.8 | 82 | 27 | 1 | 12 | 93 | 22 | 88 | 4.4e-12 | 72.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071730.t1 | A0A396GPM5 | 98.9 | 93 | 1 | 0 | 1 | 93 | 1 | 93 | 4.0e-43 | 183.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene071730.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071730.t1 | MTR_1g033340 | 46.875 | 128 | 32 | 5 | 1 | 93 | 2 | 128 | 2.74e-27 | 100 |
| MS.gene071730.t1 | MTR_3g065020 | 54.795 | 73 | 31 | 1 | 21 | 93 | 47 | 117 | 1.75e-20 | 82.4 |
| MS.gene071730.t1 | MTR_5g077370 | 48.718 | 78 | 30 | 2 | 23 | 93 | 44 | 118 | 6.94e-19 | 78.2 |
| MS.gene071730.t1 | MTR_4g006575 | 44.898 | 98 | 43 | 3 | 2 | 93 | 6 | 98 | 3.45e-18 | 76.3 |
| MS.gene071730.t1 | MTR_4g006600 | 44.898 | 98 | 43 | 3 | 2 | 93 | 6 | 98 | 3.45e-18 | 76.3 |
| MS.gene071730.t1 | MTR_2g104900 | 91.429 | 35 | 3 | 0 | 59 | 93 | 78 | 112 | 4.72e-18 | 76.3 |
| MS.gene071730.t1 | MTR_1g013130 | 47.191 | 89 | 37 | 2 | 15 | 93 | 37 | 125 | 8.74e-17 | 73.2 |
| MS.gene071730.t1 | MTR_3g106230 | 84.848 | 33 | 5 | 0 | 61 | 93 | 71 | 103 | 5.80e-16 | 70.5 |
| MS.gene071730.t1 | MTR_4g128340 | 90.625 | 32 | 3 | 0 | 62 | 93 | 45 | 76 | 9.49e-16 | 68.9 |
| MS.gene071730.t1 | MTR_1g107055 | 78.125 | 32 | 7 | 0 | 62 | 93 | 64 | 95 | 2.10e-13 | 63.2 |
| MS.gene071730.t1 | MTR_7g083210 | 78.125 | 32 | 7 | 0 | 62 | 93 | 39 | 70 | 6.92e-13 | 61.6 |
| MS.gene071730.t1 | MTR_7g086860 | 59.574 | 47 | 17 | 1 | 50 | 94 | 16 | 62 | 4.11e-12 | 58.9 |
| MS.gene071730.t1 | MTR_8g466760 | 74.194 | 31 | 8 | 0 | 62 | 92 | 98 | 128 | 8.62e-11 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene071730.t1 | AT1G66140 | 86.486 | 37 | 5 | 0 | 57 | 93 | 78 | 114 | 1.90e-18 | 77.8 |
| MS.gene071730.t1 | AT1G24625 | 93.939 | 33 | 2 | 0 | 61 | 93 | 56 | 88 | 2.77e-18 | 76.3 |
| MS.gene071730.t1 | AT1G80730 | 90.909 | 33 | 3 | 0 | 61 | 93 | 65 | 97 | 6.97e-18 | 75.5 |
| MS.gene071730.t1 | AT5G57520 | 87.879 | 33 | 4 | 0 | 61 | 93 | 49 | 81 | 1.30e-16 | 70.5 |
| MS.gene071730.t1 | AT5G10970 | 43.182 | 88 | 45 | 2 | 11 | 93 | 46 | 133 | 2.58e-15 | 69.3 |
| MS.gene071730.t1 | AT5G25160 | 71.795 | 39 | 11 | 0 | 55 | 93 | 52 | 90 | 4.24e-15 | 68.2 |
| MS.gene071730.t1 | AT5G14010 | 78.125 | 32 | 7 | 0 | 62 | 93 | 36 | 67 | 3.10e-13 | 62.0 |
| MS.gene071730.t1 | AT5G01860 | 56.098 | 41 | 18 | 0 | 62 | 102 | 65 | 105 | 1.14e-11 | 58.9 |
| MS.gene071730.t1 | AT5G27880 | 42.254 | 71 | 39 | 1 | 33 | 101 | 47 | 117 | 8.97e-11 | 57.0 |
Find 0 sgRNAs with CRISPR-Local
Find 26 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAATTACAAGAGAATATCCT+TGG | - | 32385:12103-12122 | MS.gene071730:intergenic | 25.0% |
| ATCACTTGAACCCATTAACT+CGG | - | 32385:12029-12048 | MS.gene071730:intergenic | 35.0% | |
| GAAATTGAGTGTCAAGTCAA+GGG | - | 32385:11993-12012 | MS.gene071730:intergenic | 35.0% | |
| TCTCTTGTAATTACTGCAAG+AGG | + | 32385:12110-12129 | MS.gene071730:CDS | 35.0% | |
| TGAAATTGAGTGTCAAGTCA+AGG | - | 32385:11994-12013 | MS.gene071730:intergenic | 35.0% | |
| AATATCCTTGGAGTTACCGA+TGG | - | 32385:12091-12110 | MS.gene07173:intergenic | 40.0% | |
| AGTGATGCTAGTAGTGAAGT+AGG | + | 32385:12043-12062 | MS.gene071730:CDS | 40.0% | |
| GATGACTCCAAACTTGAACT+TGG | + | 32385:11922-11941 | MS.gene071730:CDS | 40.0% | |
| GGTCATAGGATCTAGATCTT+GGG | - | 32385:11972-11991 | MS.gene071730:intergenic | 40.0% | |
| GTCATAGGATCTAGATCTTG+GGG | - | 32385:11971-11990 | MS.gene071730:intergenic | 40.0% | |
| TCATCAGAACGCTCACAAAA+GGG | + | 32385:12162-12181 | MS.gene071730:CDS | 40.0% | |
| TTCTATAGCTCGCAAGCATT+AGG | + | 32385:12136-12155 | MS.gene071730:CDS | 40.0% | |
| ! | TCAGGTTCCAAGTTCAAGTT+TGG | - | 32385:11932-11951 | MS.gene071730:intergenic | 40.0% |
| AGTGTCAAGTCAAGGGTCAT+AGG | - | 32385:11986-12005 | MS.gene071730:intergenic | 45.0% | |
| GGGTCATAGGATCTAGATCT+TGG | - | 32385:11973-11992 | MS.gene071730:intergenic | 45.0% | |
| GTCATCAGAACGCTCACAAA+AGG | + | 32385:12161-12180 | MS.gene071730:CDS | 45.0% | |
| TATAGCTCGCAAGCATTAGG+CGG | + | 32385:12139-12158 | MS.gene071730:CDS | 45.0% | |
| TGAAGTTCATGCTTCGCCAT+CGG | + | 32385:12072-12091 | MS.gene071730:CDS | 45.0% | |
| !! | GGTGCTTCTGACTGATTTTC+AGG | - | 32385:11950-11969 | MS.gene071730:intergenic | 45.0% |
| AGAACGCTCACAAAAGGGAG+AGG | + | 32385:12167-12186 | MS.gene071730:CDS | 50.0% | |
| GGCTAACGTGCAATGCGAAT+GGG | + | 32385:12195-12214 | MS.gene071730:CDS | 50.0% | |
| TCACAAAAGGGAGAGGACGA+TGG | + | 32385:12174-12193 | MS.gene071730:CDS | 50.0% | |
| TCGAGTGACGCCGAGTTAAT+GGG | + | 32385:12016-12035 | MS.gene071730:CDS | 50.0% | |
| TGGCTAACGTGCAATGCGAA+TGG | + | 32385:12194-12213 | MS.gene071730:CDS | 50.0% | |
| TTCGAGTGACGCCGAGTTAA+TGG | + | 32385:12015-12034 | MS.gene071730:CDS | 50.0% | |
| CTTCGCCATCGGTAACTCCA+AGG | + | 32385:12083-12102 | MS.gene071730:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 32385 | gene | 11920 | 12232 | 11920 | ID=MS.gene071730 |
| 32385 | mRNA | 11920 | 12232 | 11920 | ID=MS.gene071730.t1;Parent=MS.gene071730 |
| 32385 | exon | 11920 | 12232 | 11920 | ID=MS.gene071730.t1.exon1;Parent=MS.gene071730.t1 |
| 32385 | CDS | 11920 | 12232 | 11920 | ID=cds.MS.gene071730.t1;Parent=MS.gene071730.t1 |
| Gene Sequence |
| Protein sequence |
