Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07190.t1 | XP_013469195.1 | 77.3 | 194 | 35 | 2 | 1 | 185 | 1 | 194 | 2.50E-70 | 275 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07190.t1 | O82248 | 78.8 | 99 | 21 | 0 | 34 | 132 | 51 | 149 | 1.6e-41 | 170.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07190.t1 | I3TAW4 | 77.3 | 194 | 35 | 2 | 1 | 185 | 1 | 194 | 1.8e-70 | 275.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene07190.t1 | TF | NF-YB |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07190.t1 | MTR_1g088860 | 91.753 | 194 | 7 | 3 | 1 | 185 | 1 | 194 | 1.49e-125 | 351 |
| MS.gene07190.t1 | MTR_4g119500 | 78.095 | 105 | 23 | 0 | 24 | 128 | 12 | 116 | 2.60e-61 | 186 |
| MS.gene07190.t1 | MTR_0392s0020 | 68.992 | 129 | 37 | 1 | 24 | 152 | 8 | 133 | 1.43e-60 | 185 |
| MS.gene07190.t1 | MTR_1g083070 | 74.000 | 100 | 26 | 0 | 34 | 133 | 4 | 103 | 6.40e-53 | 165 |
| MS.gene07190.t1 | MTR_4g133938 | 64.407 | 118 | 39 | 1 | 35 | 152 | 20 | 134 | 1.41e-52 | 164 |
| MS.gene07190.t1 | MTR_2g026710 | 64.407 | 118 | 36 | 1 | 33 | 150 | 16 | 127 | 1.07e-51 | 163 |
| MS.gene07190.t1 | MTR_8g093920 | 72.277 | 101 | 28 | 0 | 33 | 133 | 39 | 139 | 1.81e-51 | 164 |
| MS.gene07190.t1 | MTR_7g061270 | 62.162 | 111 | 42 | 0 | 32 | 142 | 24 | 134 | 1.00e-50 | 161 |
| MS.gene07190.t1 | MTR_7g100650 | 62.162 | 111 | 42 | 0 | 32 | 142 | 24 | 134 | 1.57e-50 | 160 |
| MS.gene07190.t1 | MTR_3g058980 | 60.504 | 119 | 47 | 0 | 33 | 151 | 26 | 144 | 1.79e-50 | 161 |
| MS.gene07190.t1 | MTR_5g095740 | 71.875 | 96 | 27 | 0 | 33 | 128 | 22 | 117 | 1.25e-49 | 159 |
| MS.gene07190.t1 | MTR_8g091720 | 71.429 | 98 | 28 | 0 | 33 | 130 | 27 | 124 | 2.68e-49 | 159 |
| MS.gene07190.t1 | MTR_1g072790 | 60.714 | 112 | 44 | 0 | 33 | 144 | 28 | 139 | 8.19e-49 | 156 |
| MS.gene07190.t1 | MTR_4g133952 | 59.829 | 117 | 44 | 2 | 13 | 128 | 38 | 152 | 4.82e-48 | 156 |
| MS.gene07190.t1 | MTR_1g039040 | 56.604 | 106 | 46 | 0 | 32 | 137 | 4 | 109 | 1.77e-42 | 140 |
| MS.gene07190.t1 | MTR_1g029070 | 44.954 | 109 | 60 | 0 | 19 | 127 | 37 | 145 | 3.91e-28 | 103 |
| MS.gene07190.t1 | MTR_5g095900 | 44.565 | 92 | 51 | 0 | 31 | 122 | 13 | 104 | 3.88e-21 | 85.9 |
| MS.gene07190.t1 | MTR_1g029100 | 40.000 | 80 | 48 | 0 | 39 | 118 | 7 | 86 | 2.08e-18 | 76.3 |
| MS.gene07190.t1 | MTR_2g056000 | 32.727 | 110 | 72 | 1 | 3 | 112 | 12 | 119 | 1.54e-17 | 76.6 |
| MS.gene07190.t1 | MTR_1g028480 | 46.875 | 64 | 34 | 0 | 64 | 127 | 19 | 82 | 3.81e-16 | 70.9 |
| MS.gene07190.t1 | MTR_4g112380 | 32.051 | 78 | 53 | 0 | 35 | 112 | 12 | 89 | 3.45e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07190.t1 | AT2G47810 | 77.451 | 102 | 23 | 0 | 31 | 132 | 48 | 149 | 4.46e-58 | 179 |
| MS.gene07190.t1 | AT1G09030 | 57.143 | 140 | 56 | 1 | 32 | 171 | 1 | 136 | 8.22e-53 | 165 |
| MS.gene07190.t1 | AT5G47640 | 68.468 | 111 | 32 | 1 | 33 | 140 | 26 | 136 | 2.19e-52 | 166 |
| MS.gene07190.t1 | AT4G14540 | 75.000 | 96 | 24 | 0 | 33 | 128 | 20 | 115 | 6.14e-52 | 164 |
| MS.gene07190.t1 | AT2G38880 | 64.865 | 111 | 38 | 1 | 32 | 141 | 19 | 129 | 2.92e-51 | 161 |
| MS.gene07190.t1 | AT2G13570 | 73.267 | 101 | 27 | 0 | 33 | 133 | 35 | 135 | 2.99e-51 | 164 |
| MS.gene07190.t1 | AT2G38880 | 64.865 | 111 | 38 | 1 | 32 | 141 | 19 | 129 | 3.83e-51 | 161 |
| MS.gene07190.t1 | AT2G38880 | 64.865 | 111 | 38 | 1 | 32 | 141 | 19 | 129 | 3.83e-51 | 161 |
| MS.gene07190.t1 | AT2G38880 | 64.865 | 111 | 38 | 1 | 32 | 141 | 19 | 129 | 3.83e-51 | 161 |
| MS.gene07190.t1 | AT3G53340 | 50.336 | 149 | 67 | 1 | 32 | 180 | 27 | 168 | 5.78e-51 | 162 |
| MS.gene07190.t1 | AT3G53340 | 50.336 | 149 | 67 | 1 | 32 | 180 | 27 | 168 | 5.78e-51 | 162 |
| MS.gene07190.t1 | AT2G38880 | 65.455 | 110 | 37 | 1 | 32 | 141 | 19 | 127 | 3.30e-50 | 159 |
| MS.gene07190.t1 | AT2G37060 | 63.063 | 111 | 41 | 0 | 32 | 142 | 28 | 138 | 4.76e-50 | 159 |
| MS.gene07190.t1 | AT2G37060 | 63.063 | 111 | 41 | 0 | 32 | 142 | 28 | 138 | 4.76e-50 | 159 |
| MS.gene07190.t1 | AT2G37060 | 63.063 | 111 | 41 | 0 | 32 | 142 | 28 | 138 | 4.76e-50 | 159 |
| MS.gene07190.t1 | AT2G38880 | 73.118 | 93 | 25 | 0 | 32 | 124 | 19 | 111 | 2.35e-49 | 155 |
| MS.gene07190.t1 | AT2G38880 | 73.118 | 93 | 25 | 0 | 32 | 124 | 19 | 111 | 6.14e-49 | 156 |
| MS.gene07190.t1 | AT2G38880 | 73.118 | 93 | 25 | 0 | 32 | 124 | 19 | 111 | 6.14e-49 | 156 |
| MS.gene07190.t1 | AT3G53340 | 72.043 | 93 | 26 | 0 | 32 | 124 | 27 | 119 | 6.93e-49 | 155 |
| MS.gene07190.t1 | AT3G53340 | 69.697 | 99 | 29 | 1 | 32 | 130 | 27 | 124 | 8.81e-49 | 155 |
| MS.gene07190.t1 | AT3G53340 | 69.697 | 99 | 29 | 1 | 32 | 130 | 27 | 124 | 8.81e-49 | 155 |
| MS.gene07190.t1 | AT2G38880 | 73.118 | 93 | 25 | 0 | 32 | 124 | 19 | 111 | 9.38e-49 | 155 |
| MS.gene07190.t1 | AT2G38880 | 73.118 | 93 | 25 | 0 | 32 | 124 | 19 | 111 | 9.38e-49 | 155 |
| MS.gene07190.t1 | AT2G38880 | 73.118 | 93 | 25 | 0 | 32 | 124 | 19 | 111 | 9.38e-49 | 155 |
| MS.gene07190.t1 | AT5G47670 | 63.265 | 98 | 36 | 0 | 32 | 129 | 27 | 124 | 6.42e-46 | 150 |
| MS.gene07190.t1 | AT5G47670 | 62.626 | 99 | 37 | 0 | 31 | 129 | 55 | 153 | 1.23e-45 | 150 |
| MS.gene07190.t1 | AT5G47670 | 62.626 | 99 | 37 | 0 | 31 | 129 | 55 | 153 | 1.23e-45 | 150 |
| MS.gene07190.t1 | AT1G21970 | 50.862 | 116 | 57 | 0 | 19 | 134 | 44 | 159 | 2.97e-41 | 139 |
| MS.gene07190.t1 | AT5G23090 | 35.052 | 97 | 61 | 1 | 35 | 129 | 12 | 108 | 1.45e-17 | 75.9 |
| MS.gene07190.t1 | AT5G23090 | 38.462 | 78 | 48 | 0 | 35 | 112 | 12 | 89 | 1.22e-16 | 73.6 |
| MS.gene07190.t1 | AT5G23090 | 38.462 | 78 | 48 | 0 | 35 | 112 | 12 | 89 | 1.22e-16 | 73.6 |
| MS.gene07190.t1 | AT5G23090 | 38.462 | 78 | 48 | 0 | 35 | 112 | 12 | 89 | 1.22e-16 | 73.6 |
| MS.gene07190.t1 | AT5G08190 | 32.292 | 96 | 63 | 1 | 35 | 128 | 12 | 107 | 4.90e-16 | 72.0 |
| MS.gene07190.t1 | AT5G08190 | 34.615 | 78 | 51 | 0 | 35 | 112 | 12 | 89 | 6.85e-15 | 69.3 |
Find 35 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CATGGTTTCTTTAGCATCTT+TGG | 0.164527 | 1.4:+70537667 | None:intergenic |
| CCTCTTCTATGTTCAATAAT+AGG | 0.214525 | 1.4:-70537337 | MS.gene07190:CDS |
| GATGATGTTTGTTGGGCTTT+GGG | 0.221205 | 1.4:-70537557 | MS.gene07190:CDS |
| TGATGTTGTTACCCTTATTT+TGG | 0.229982 | 1.4:+70537453 | None:intergenic |
| TGATGATGTTTGTTGGGCTT+TGG | 0.269661 | 1.4:-70537558 | MS.gene07190:CDS |
| GGAAAGAGGGAACCAAAATA+AGG | 0.312778 | 1.4:-70537465 | MS.gene07190:CDS |
| TGTTGGGCTTTGGGGACACT+AGG | 0.347462 | 1.4:-70537548 | MS.gene07190:CDS |
| AAAGGTATTTGTATAAGTAT+AGG | 0.352771 | 1.4:-70537499 | MS.gene07190:CDS |
| TTTGTATAAGTATAGGGAAT+TGG | 0.359649 | 1.4:-70537492 | MS.gene07190:CDS |
| GAAGCAACATTCACTTTCCT+TGG | 0.401544 | 1.4:+70537359 | None:intergenic |
| ATCCGACCAACATTAGCTAT+TGG | 0.403010 | 1.4:+70537725 | None:intergenic |
| AAGGTATTTGTATAAGTATA+GGG | 0.417767 | 1.4:-70537498 | MS.gene07190:CDS |
| GAATGGTGATGATGTTTGTT+GGG | 0.429389 | 1.4:-70537564 | MS.gene07190:CDS |
| GAAACATATGATTATGAAGA+TGG | 0.453321 | 1.4:-70537395 | MS.gene07190:CDS |
| ACATGGGTGATAACAACAAA+GGG | 0.487792 | 1.4:-70537844 | MS.gene07190:CDS |
| GAAAGAGGGAACCAAAATAA+GGG | 0.501997 | 1.4:-70537464 | MS.gene07190:CDS |
| GTTGGGCTTTGGGGACACTA+GGG | 0.503961 | 1.4:-70537547 | MS.gene07190:CDS |
| ATGATGTTTGTTGGGCTTTG+GGG | 0.505210 | 1.4:-70537556 | MS.gene07190:CDS |
| TGAATGGTGATGATGTTTGT+TGG | 0.510123 | 1.4:-70537565 | MS.gene07190:CDS |
| CTCTGAGACACACTCTTGCA+TGG | 0.513185 | 1.4:+70537649 | None:intergenic |
| GAGAAGCGCAAGACAGTGAA+TGG | 0.520201 | 1.4:-70537581 | MS.gene07190:CDS |
| AGGGAATTGGATGCGGAAAG+AGG | 0.523773 | 1.4:-70537479 | MS.gene07190:CDS |
| TTGCTTCCAATAGCTAATGT+TGG | 0.538809 | 1.4:-70537731 | MS.gene07190:CDS |
| TTCAAAATCCCCTTGACTGA+AGG | 0.540445 | 1.4:+70537306 | None:intergenic |
| GGGAATTGGATGCGGAAAGA+GGG | 0.541973 | 1.4:-70537478 | MS.gene07190:CDS |
| CCTATTATTGAACATAGAAG+AGG | 0.550260 | 1.4:+70537337 | None:intergenic |
| TAAGTATAGGGAATTGGATG+CGG | 0.557603 | 1.4:-70537486 | MS.gene07190:CDS |
| ATTACGCTGAGCCACTCAAA+AGG | 0.563009 | 1.4:-70537517 | MS.gene07190:CDS |
| GAACAGTGACCTTCAGTCAA+GGG | 0.574883 | 1.4:-70537315 | MS.gene07190:CDS |
| TTCCAATAGCTAATGTTGGT+CGG | 0.585707 | 1.4:-70537727 | MS.gene07190:CDS |
| ATGGTAAGTCACAAACTCCA+AGG | 0.613914 | 1.4:-70537376 | MS.gene07190:CDS |
| AACAGTGACCTTCAGTCAAG+GGG | 0.614235 | 1.4:-70537314 | MS.gene07190:CDS |
| AACATGGGTGATAACAACAA+AGG | 0.619511 | 1.4:-70537845 | MS.gene07190:CDS |
| GGAACAGTGACCTTCAGTCA+AGG | 0.622121 | 1.4:-70537316 | MS.gene07190:CDS |
| AGCATCTGACAAGTGTCACA+AGG | 0.642405 | 1.4:-70537603 | MS.gene07190:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | AAAGGTATTTGTATAAGTAT+AGG | - | chr1.4:70537650-70537669 | MS.gene07190:CDS | 20.0% |
| !!! | AAGGTATTTGTATAAGTATA+GGG | - | chr1.4:70537651-70537670 | MS.gene07190:CDS | 20.0% |
| ! | GAAACATATGATTATGAAGA+TGG | - | chr1.4:70537754-70537773 | MS.gene07190:CDS | 25.0% |
| ! | TTTGTATAAGTATAGGGAAT+TGG | - | chr1.4:70537657-70537676 | MS.gene07190:CDS | 25.0% |
| !! | TTATACAAATACCTTTTGAG+TGG | + | chr1.4:70537646-70537665 | None:intergenic | 25.0% |
| CCTATTATTGAACATAGAAG+AGG | + | chr1.4:70537815-70537834 | None:intergenic | 30.0% | |
| CCTCTTCTATGTTCAATAAT+AGG | - | chr1.4:70537812-70537831 | MS.gene07190:CDS | 30.0% | |
| !! | TCTTTGGAGATTTTTGCATT+TGG | + | chr1.4:70537469-70537488 | None:intergenic | 30.0% |
| !! | TGATGTTGTTACCCTTATTT+TGG | + | chr1.4:70537699-70537718 | None:intergenic | 30.0% |
| !!! | GAGTTTGTTAGTTTTGTAAC+TGG | - | chr1.4:70537520-70537539 | MS.gene07190:CDS | 30.0% |
| CATGGTTTCTTTAGCATCTT+TGG | + | chr1.4:70537485-70537504 | None:intergenic | 35.0% | |
| GAAAGAGGGAACCAAAATAA+GGG | - | chr1.4:70537685-70537704 | MS.gene07190:CDS | 35.0% | |
| TAAGTATAGGGAATTGGATG+CGG | - | chr1.4:70537663-70537682 | MS.gene07190:CDS | 35.0% | |
| TTCCAATAGCTAATGTTGGT+CGG | - | chr1.4:70537422-70537441 | MS.gene07190:CDS | 35.0% | |
| TTGCTTCCAATAGCTAATGT+TGG | - | chr1.4:70537418-70537437 | MS.gene07190:CDS | 35.0% | |
| ! | GAATGGTGATGATGTTTGTT+GGG | - | chr1.4:70537585-70537604 | MS.gene07190:CDS | 35.0% |
| ! | TGAATGGTGATGATGTTTGT+TGG | - | chr1.4:70537584-70537603 | MS.gene07190:CDS | 35.0% |
| ! | TTGGAGATTTTTGCATTTGG+TGG | + | chr1.4:70537466-70537485 | None:intergenic | 35.0% |
| ATCCGACCAACATTAGCTAT+TGG | + | chr1.4:70537427-70537446 | None:intergenic | 40.0% | |
| ATGGTAAGTCACAAACTCCA+AGG | - | chr1.4:70537773-70537792 | MS.gene07190:CDS | 40.0% | |
| GAAGCAACATTCACTTTCCT+TGG | + | chr1.4:70537793-70537812 | None:intergenic | 40.0% | |
| GGAAAGAGGGAACCAAAATA+AGG | - | chr1.4:70537684-70537703 | MS.gene07190:CDS | 40.0% | |
| ! | ATGATGTTTGTTGGGCTTTG+GGG | - | chr1.4:70537593-70537612 | MS.gene07190:CDS | 40.0% |
| ! | GATGATGTTTGTTGGGCTTT+GGG | - | chr1.4:70537592-70537611 | MS.gene07190:CDS | 40.0% |
| ! | TGATGATGTTTGTTGGGCTT+TGG | - | chr1.4:70537591-70537610 | MS.gene07190:CDS | 40.0% |
| AACAGTGACCTTCAGTCAAG+GGG | - | chr1.4:70537835-70537854 | MS.gene07190:CDS | 45.0% | |
| AGCATCTGACAAGTGTCACA+AGG | - | chr1.4:70537546-70537565 | MS.gene07190:CDS | 45.0% | |
| ATTACGCTGAGCCACTCAAA+AGG | - | chr1.4:70537632-70537651 | MS.gene07190:CDS | 45.0% | |
| GAACAGTGACCTTCAGTCAA+GGG | - | chr1.4:70537834-70537853 | MS.gene07190:CDS | 45.0% | |
| AGGGAATTGGATGCGGAAAG+AGG | - | chr1.4:70537670-70537689 | MS.gene07190:CDS | 50.0% | |
| GGAACAGTGACCTTCAGTCA+AGG | - | chr1.4:70537833-70537852 | MS.gene07190:CDS | 50.0% | |
| GGGAATTGGATGCGGAAAGA+GGG | - | chr1.4:70537671-70537690 | MS.gene07190:CDS | 50.0% | |
| ! | GAGAAGCGCAAGACAGTGAA+TGG | - | chr1.4:70537568-70537587 | MS.gene07190:CDS | 50.0% |
| !! | CTCTGAGACACACTCTTGCA+TGG | + | chr1.4:70537503-70537522 | None:intergenic | 50.0% |
| !! | GTTGGGCTTTGGGGACACTA+GGG | - | chr1.4:70537602-70537621 | MS.gene07190:CDS | 55.0% |
| !! | TGTTGGGCTTTGGGGACACT+AGG | - | chr1.4:70537601-70537620 | MS.gene07190:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 70537307 | 70537864 | 70537307 | ID=MS.gene07190 |
| chr1.4 | mRNA | 70537307 | 70537864 | 70537307 | ID=MS.gene07190.t1;Parent=MS.gene07190 |
| chr1.4 | exon | 70537307 | 70537864 | 70537307 | ID=MS.gene07190.t1.exon1;Parent=MS.gene07190.t1 |
| chr1.4 | CDS | 70537307 | 70537864 | 70537307 | ID=cds.MS.gene07190.t1;Parent=MS.gene07190.t1 |
| Gene Sequence |
| Protein sequence |
