AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072060

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072060.t1 MTR_2g006150 91.538 520 11 10 1 520 1 487 0.0 917
MS.gene072060.t1 MTR_2g006150 93.966 464 8 9 1 464 1 444 0.0 845
MS.gene072060.t1 MTR_5g039600 92.045 88 6 1 131 217 61 148 2.69e-49 175
MS.gene072060.t1 MTR_4g108370 65.909 132 33 4 77 205 44 166 6.13e-44 161
MS.gene072060.t1 MTR_7g028160 52.632 152 62 3 108 255 21 166 2.38e-38 142
MS.gene072060.t1 MTR_1g114380 80.488 82 13 1 127 208 27 105 6.18e-38 140
MS.gene072060.t1 MTR_1g101810 57.500 120 45 2 106 224 38 152 3.15e-36 137
MS.gene072060.t1 MTR_1g038650 76.190 84 17 1 121 204 20 100 3.88e-36 134
MS.gene072060.t1 MTR_1g101810 57.500 120 45 2 106 224 68 182 8.20e-36 137
MS.gene072060.t1 MTR_8g033070 78.947 76 16 0 133 208 77 152 2.25e-35 135
MS.gene072060.t1 MTR_1g063870 80.282 71 14 0 128 198 41 111 9.74e-35 130
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072060.t1 AT1G58100 46.824 425 129 17 77 477 13 364 7.13e-81 258
MS.gene072060.t1 AT1G35560 43.027 337 94 10 133 463 52 296 2.02e-59 200
MS.gene072060.t1 AT1G58100 41.647 425 127 19 77 477 13 340 2.62e-57 196
MS.gene072060.t1 AT1G72010 40.878 296 102 10 127 399 54 299 5.56e-48 171
MS.gene072060.t1 AT1G69690 58.599 157 38 3 131 275 46 187 1.36e-41 152
MS.gene072060.t1 AT5G23280 76.190 84 17 1 125 208 31 111 4.59e-37 137
MS.gene072060.t1 AT2G45680 57.600 125 41 3 90 213 39 152 4.35e-36 138
MS.gene072060.t1 AT5G08330 78.750 80 14 1 127 206 25 101 1.25e-35 133
MS.gene072060.t1 AT3G27010 53.600 125 42 2 73 197 31 139 7.54e-35 133
MS.gene072060.t1 AT3G27010 53.600 125 42 2 73 197 31 139 7.54e-35 133
MS.gene072060.t1 AT5G51910 78.205 78 16 1 132 208 52 129 7.98e-34 130
MS.gene072060.t1 AT5G51910 78.205 78 16 1 132 208 52 129 7.98e-34 130
MS.gene072060.t1 AT2G37000 80.000 60 12 0 136 195 43 102 3.36e-27 108
MS.gene072060.t1 AT5G41030 51.546 97 35 1 133 229 65 149 1.55e-26 108
MS.gene072060.t1 AT3G45150 61.905 63 24 0 133 195 14 76 1.24e-21 92.4

Find 151 sgRNAs with CRISPR-Local

Find 164 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAGGCCATCACCTTTCATTA+TGG 0.235639 2.4:+76169601 MS.gene072060:CDS
CTTGTTGTTGGTGGTGGGGA+TGG 0.241546 2.4:-76169523 None:intergenic
AGGTTCAATGTTCCAGCTTC+TGG 0.242129 2.4:+76170727 MS.gene072060:CDS
GTTTGAAGAAGGTTGAATAT+TGG 0.260266 2.4:-76169633 None:intergenic
TTGAAGAAAATGGTTGAATT+TGG 0.273936 2.4:-76169802 None:intergenic
TCACCAACAACAGGATGATA+TGG 0.275830 2.4:+76170411 MS.gene072060:CDS
TTGTTGTTGGTGGTGGGGAT+GGG 0.292871 2.4:-76169522 None:intergenic
TTCCAGTTGACCAGAGAATT+AGG 0.308053 2.4:+76169944 MS.gene072060:CDS
GATAGTCATTGTGAGGTATT+AGG 0.316778 2.4:-76171009 None:intergenic
GACGGTGGTGGAGGAGGTGG+TGG 0.325996 2.4:+76170442 MS.gene072060:CDS
AATTTGGTTGTTGTTGTTGT+TGG 0.326907 2.4:-76169786 None:intergenic
GACTGAACATGAGGTGTAAA+AGG 0.331793 2.4:-76170649 None:intergenic
ATGATTCTTGTTGTTGGTGG+TGG 0.333014 2.4:-76169529 None:intergenic
CGGGGAGTGGAAGTACAATT+TGG 0.342926 2.4:+76170554 MS.gene072060:CDS
TCTTCTCCACTCTCATCATT+AGG 0.347346 2.4:-76170982 None:intergenic
TGTTGTTGGTGGTGGGGATG+GGG 0.348885 2.4:-76169521 None:intergenic
CGCCCATATCATCCTGTTGT+TGG 0.355044 2.4:-76170414 None:intergenic
AGGCCATCACCTTTCATTAT+GGG 0.359459 2.4:+76169602 MS.gene072060:CDS
GTGGGGATGGGGATGAATAT+TGG 0.363141 2.4:-76169510 None:intergenic
GAAGGTGACGGTGGTGGAGG+AGG 0.367290 2.4:+76170436 MS.gene072060:CDS
GGCTGGGATTGTTCCGGTGC+CGG 0.367521 2.4:-76170035 None:intergenic
CTGTCCTTGGTGGATCGTTT+TGG 0.369093 2.4:-76169857 None:intergenic
TACCAACACGCTCCTCTGTT+AGG 0.370083 2.4:+76170187 MS.gene072060:CDS
GTTGTTGCTGTTGCTGTTGT+TGG 0.372670 2.4:-76170231 None:intergenic
TGGACGTGGTGGTTTACAAT+TGG 0.382459 2.4:+76170777 MS.gene072060:CDS
TGATTCTTGTTGTTGGTGGT+GGG 0.388715 2.4:-76169528 None:intergenic
GTATCAACCATCACAGCATT+TGG 0.390115 2.4:+76170807 MS.gene072060:CDS
GCGGTTGAAGTTGAAGAAAA+TGG 0.393103 2.4:-76169812 None:intergenic
GGCCTAATTCTCTGGTCAAC+TGG 0.396838 2.4:-76169946 None:intergenic
GTAGCCCACATGGCTGTTGC+CGG 0.398412 2.4:-76170529 None:intergenic
GGATTTGTGGCCTAATTCTC+TGG 0.401966 2.4:-76169954 None:intergenic
GATTCTTGTTGTTGGTGGTG+GGG 0.404293 2.4:-76169527 None:intergenic
GGTGGTGGAGGAGGTGGTGG+TGG 0.404546 2.4:+76170445 MS.gene072060:CDS
GTGAGGATGATTCTTGTTGT+TGG 0.411900 2.4:-76169535 None:intergenic
TTGTTTCACCATCGGATTTG+TGG 0.412133 2.4:-76169967 None:intergenic
CCACTCTCATCATTAGGTTG+AGG 0.418843 2.4:-76170976 None:intergenic
GGTGACGGTGGTGGAGGAGG+TGG 0.419720 2.4:+76170439 MS.gene072060:CDS
TATCAACCATCACAGCATTT+GGG 0.420346 2.4:+76170808 MS.gene072060:CDS
GGACGTGGTGGTTTACAATT+GGG 0.421245 2.4:+76170778 MS.gene072060:CDS
TCAACAACATTCAATGTCAT+TGG 0.422221 2.4:+76170933 MS.gene072060:CDS
TTGTTGTTGTTGGATACATC+TGG 0.423257 2.4:-76169776 None:intergenic
GACGTTGAGCGTTGAGAAAT+TGG 0.424642 2.4:-76170056 None:intergenic
AAGAGTTGTTAACGTTGAAA+TGG 0.427409 2.4:-76170894 None:intergenic
GAGGGAGAAGAGTTTGAAGA+AGG 0.435751 2.4:-76169644 None:intergenic
GGTGGTGGTGTTGAATTTCA+AGG 0.437658 2.4:+76170754 MS.gene072060:CDS
CAGATCTGGTGGCAATTGCC+AGG 0.437859 2.4:-76169742 None:intergenic
TCATCATTAGGTTGAGGTTG+AGG 0.438959 2.4:-76170970 None:intergenic
CTGATGAACGCTGATCAAAT+TGG 0.440741 2.4:+76170256 MS.gene072060:CDS
GGGATGGGGATGAATATTGG+TGG 0.440791 2.4:-76169507 None:intergenic
GAAATTGGCTGGGATTGTTC+CGG 0.446496 2.4:-76170041 None:intergenic
GGCGAAGGTGACGGTGGTGG+AGG 0.448389 2.4:+76170433 MS.gene072060:CDS
GGCTGTATCTGATTCCAACT+TGG 0.449042 2.4:+76170834 MS.gene072060:CDS
ACAGCCATGTGGGCTACAGC+GGG 0.455998 2.4:+76170535 MS.gene072060:CDS
GGAGCTTCTTCTTCTTCTGT+AGG 0.458812 2.4:+76170466 MS.gene072060:CDS
ACCAACACGCTCCTCTGTTA+GGG 0.462020 2.4:+76170188 MS.gene072060:CDS
GGTGATGGCCTTCCATCAAA+AGG 0.462353 2.4:-76169590 None:intergenic
AATGTTCCAGCTTCTGGTGG+TGG 0.463360 2.4:+76170733 MS.gene072060:CDS
CTCAGTTACATTTCATGCCA+AGG 0.466286 2.4:+76170707 MS.gene072060:CDS
CAAAACAAATCACCAACAAC+AGG 0.468328 2.4:+76170402 MS.gene072060:CDS
AGGATTTGCAACCGCGGTGG+CGG 0.472490 2.4:-76169834 None:intergenic
ATTTGCAACCGCGGTGGCGG+CGG 0.474554 2.4:-76169831 None:intergenic
CCATTCAATTGTTTCACCAT+CGG 0.476071 2.4:-76169975 None:intergenic
TTGAGCGTTGAGAAATTGGC+TGG 0.476634 2.4:-76170052 None:intergenic
CCAGCTTCTGGTGGTGGTGG+TGG 0.477601 2.4:+76170739 MS.gene072060:CDS
ACACCTCATTGATGCTTCCC+TGG 0.479655 2.4:+76169724 MS.gene072060:CDS
GCTTCTTCAACAAGCGGAGC+CGG 0.481221 2.4:+76169997 MS.gene072060:CDS
TCGACCGTCAAATATGTTAC+CGG 0.485929 2.4:+76170510 MS.gene072060:CDS
ATCATCGCTGCCACCGGCAC+CGG 0.486326 2.4:+76170022 MS.gene072060:CDS
ATGGGCGAAGGTGACGGTGG+TGG 0.487166 2.4:+76170430 MS.gene072060:CDS
AGGATGATTCTTGTTGTTGG+TGG 0.493938 2.4:-76169532 None:intergenic
ATGTGGGCTACAGCGGGGAG+TGG 0.494234 2.4:+76170541 MS.gene072060:CDS
GCTGTATCTGATTCCAACTT+GGG 0.503244 2.4:+76170835 MS.gene072060:CDS
ACGAGAATTACATGAGAAAA+CGG 0.506668 2.4:+76170293 MS.gene072060:CDS
CTTAATGCTTATACAAGAGC+TGG 0.507717 2.4:+76170871 MS.gene072060:CDS
CATGTTCAGTCTCAAAATCA+AGG 0.508213 2.4:+76170661 MS.gene072060:CDS
AGCAGCAAACATACCCAAGT+TGG 0.510675 2.4:-76170848 None:intergenic
ACCATCACAGCATTTGGGAT+TGG 0.511721 2.4:+76170813 MS.gene072060:CDS
GTTCCAGCTTCTGGTGGTGG+TGG 0.513092 2.4:+76170736 MS.gene072060:CDS
CCGGCGATCATCGCTGCCAC+CGG 0.513461 2.4:+76170016 MS.gene072060:CDS
CAAAAGTTGACGGCCGTGGC+CGG 0.517328 2.4:+76169885 MS.gene072060:CDS
CCGGTGGCAGCGATGATCGC+CGG 0.518014 2.4:-76170016 None:intergenic
CAAAATGAAAGTGGCGGTGA+TGG 0.520198 2.4:+76170352 MS.gene072060:CDS
GTTGCTGTTGCTGTTGTTGG+TGG 0.522829 2.4:-76170228 None:intergenic
TGGTACGCAGCTTCTTCGTA+AGG 0.525067 2.4:-76170169 None:intergenic
TTCAATGTTCCAGCTTCTGG+TGG 0.527514 2.4:+76170730 MS.gene072060:CDS
CACCAACAACAGGATGATAT+GGG 0.530307 2.4:+76170412 MS.gene072060:CDS
CCGATGGTGAAACAATTGAA+TGG 0.532182 2.4:+76169975 MS.gene072060:CDS
GCATTCGAATTCTCCGGCCA+CGG 0.535337 2.4:-76169898 None:intergenic
TGCTGATGATGAGAATTTGA+AGG 0.536098 2.4:-76169557 None:intergenic
TCGCCGGCATTCGAATTCTC+CGG 0.537835 2.4:-76169904 None:intergenic
AACAACAATCAAAATGAAAG+TGG 0.537872 2.4:+76170343 MS.gene072060:CDS
CACACAAAAGTTGACGGCCG+TGG 0.538934 2.4:+76169881 MS.gene072060:CDS
TGGAAGAGGAGGTTCAGATC+TGG 0.540562 2.4:-76169756 None:intergenic
TGTTGTTGGTGGTGTTGATG+AGG 0.542903 2.4:-76170217 None:intergenic
ACAGTGTAGAGCCGCTGCTA+CGG 0.544103 2.4:-76170084 None:intergenic
AGAAGGTTGAATATTGGAGA+TGG 0.548864 2.4:-76169627 None:intergenic
GACAGGCACACAAAAGTTGA+CGG 0.553080 2.4:+76169875 MS.gene072060:CDS
TTGTTGGATACATCTGGAAG+AGG 0.554700 2.4:-76169770 None:intergenic
ACTCCCCGCTGTAGCCCACA+TGG 0.560090 2.4:-76170539 None:intergenic
GTGATGGCCTTCCATCAAAA+GGG 0.560570 2.4:-76169589 None:intergenic
AACAGCCATGTGGGCTACAG+CGG 0.560758 2.4:+76170534 MS.gene072060:CDS
GTTGCCGGTAACATATTTGA+CGG 0.564703 2.4:-76170514 None:intergenic
CAAAACGATCCACCAAGGAC+AGG 0.572544 2.4:+76169858 MS.gene072060:CDS
GATGATATGGGCGAAGGTGA+CGG 0.576236 2.4:+76170424 MS.gene072060:CDS
TGGGATTGTTCCGGTGCCGG+TGG 0.577716 2.4:-76170032 None:intergenic
GTTGAATTTCAAGGTGGACG+TGG 0.582295 2.4:+76170763 MS.gene072060:CDS
ACTCTTGCTGCACATGTCGC+CGG 0.582995 2.4:-76169920 None:intergenic
GTTGCAGCACCACCACTCAC+TGG 0.588807 2.4:-76170583 None:intergenic
TTCTCATCATCAGCAGCTTG+TGG 0.593589 2.4:+76169565 MS.gene072060:CDS
TGGCCGGAGAATTCGAATGC+CGG 0.599117 2.4:+76169901 MS.gene072060:CDS
GAGATGGAGCCCATAATGAA+AGG 0.601517 2.4:-76169611 None:intergenic
GAGCCCATAATGAAAGGTGA+TGG 0.605278 2.4:-76169605 None:intergenic
TTGGATACATCTGGAAGAGG+AGG 0.605386 2.4:-76169767 None:intergenic
TGAATGGCTTCTTCAACAAG+CGG 0.607294 2.4:+76169991 MS.gene072060:CDS
ATGATGAGAATTTGAAGGTG+AGG 0.608155 2.4:-76169552 None:intergenic
GGCATGAAATGTAACTGAGA+GGG 0.612083 2.4:-76170703 None:intergenic
GCCAATCCCAAATGCTGTGA+TGG 0.613902 2.4:-76170814 None:intergenic
TGGAGGATTTGCAACCGCGG+TGG 0.616693 2.4:-76169837 None:intergenic
GAAGCTGGAACATTGAACCT+TGG 0.618817 2.4:-76170724 None:intergenic
GAGTGGAAGTACAATTTGGA+TGG 0.623430 2.4:+76170558 MS.gene072060:CDS
CAAAACAACAAGCAGAAGAG+AGG 0.623869 2.4:+76169482 MS.gene072060:CDS
GATATGGGCGAAGGTGACGG+TGG 0.625298 2.4:+76170427 MS.gene072060:CDS
AGATCTGGTGGCAATTGCCA+GGG 0.625421 2.4:-76169741 None:intergenic
CCTCAACCTAATGATGAGAG+TGG 0.625980 2.4:+76170976 MS.gene072060:CDS
CAACAGGATGATATGGGCGA+AGG 0.628122 2.4:+76170418 MS.gene072060:CDS
AACTTCAACCGCCGCCACCG+CGG 0.629590 2.4:+76169823 MS.gene072060:CDS
GAAAGTGGCGGTGATGGTGA+TGG 0.632003 2.4:+76170358 MS.gene072060:CDS
AAGAGGAGGTTCAGATCTGG+TGG 0.633145 2.4:-76169753 None:intergenic
AACGTCTCACTCCGTAGCAG+CGG 0.639266 2.4:+76170073 MS.gene072060:CDS
ACCCTAACAGAGGAGCGTGT+TGG 0.640297 2.4:-76170189 None:intergenic
TGAGCGTTGAGAAATTGGCT+GGG 0.640396 2.4:-76170051 None:intergenic
GAATTAGGCCACAAATCCGA+TGG 0.643115 2.4:+76169959 MS.gene072060:CDS
AGCATCAATGAGGTGTTGAG+TGG 0.643841 2.4:-76169717 None:intergenic
CTGAGAGGAGCTGATTTCGA+CGG 0.644392 2.4:-76170115 None:intergenic
AACAATCAAAATGAAAGTGG+CGG 0.649002 2.4:+76170346 MS.gene072060:CDS
GCATCAATGAGGTGTTGAGT+GGG 0.650961 2.4:-76169716 None:intergenic
GTTACCGGCAACAGCCATGT+GGG 0.654057 2.4:+76170525 MS.gene072060:CDS
TGTTACCGGCAACAGCCATG+TGG 0.658913 2.4:+76170524 MS.gene072060:CDS
GGTAGTAGATAGTCATTGTG+AGG 0.659915 2.4:-76171016 None:intergenic
GAATTTCAAGGTGGACGTGG+TGG 0.660159 2.4:+76170766 MS.gene072060:CDS
TGGCATGAAATGTAACTGAG+AGG 0.665182 2.4:-76170704 None:intergenic
TCCTCCAAAACGATCCACCA+AGG 0.665860 2.4:+76169853 MS.gene072060:CDS
CAGCCATGTGGGCTACAGCG+GGG 0.667527 2.4:+76170536 MS.gene072060:CDS
AGAGGAGCTGATTTCGACGG+CGG 0.670392 2.4:-76170112 None:intergenic
AGACACAGACACAAAACATG+TGG 0.683636 2.4:+76170626 MS.gene072060:CDS
TTGCCAGGGAAGCATCAATG+AGG 0.690178 2.4:-76169727 None:intergenic
ATTTGGATGGTACCAGTGAG+TGG 0.695345 2.4:+76170571 MS.gene072060:CDS
TGAGGATGAAACCCTAACAG+AGG 0.710378 2.4:-76170199 None:intergenic
TGGATGGTACCAGTGAGTGG+TGG 0.715883 2.4:+76170574 MS.gene072060:CDS
AGCGCGCTATGAAAACTGAG+AGG 0.721312 2.4:-76170130 None:intergenic
GGTGGTGTTGAATTTCAAGG+TGG 0.744371 2.4:+76170757 MS.gene072060:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TGATTTGTTTTGAAACTTTT+GGG - chr2.4:76170394-76170413 None:intergenic 20.0%
! AACAACAATCAAAATGAAAG+TGG + chr2.4:76170343-76170362 MS.gene072060:CDS 25.0%
! TTGAAGAAAATGGTTGAATT+TGG - chr2.4:76169805-76169824 None:intergenic 25.0%
!!! GTGATTTGTTTTGAAACTTT+TGG - chr2.4:76170395-76170414 None:intergenic 25.0%
AACAATCAAAATGAAAGTGG+CGG + chr2.4:76170346-76170365 MS.gene072060:CDS 30.0%
AAGAGTTGTTAACGTTGAAA+TGG - chr2.4:76170897-76170916 None:intergenic 30.0%
ACGAGAATTACATGAGAAAA+CGG + chr2.4:76170293-76170312 MS.gene072060:CDS 30.0%
GTTTGAAGAAGGTTGAATAT+TGG - chr2.4:76169636-76169655 None:intergenic 30.0%
TCAACAACATTCAATGTCAT+TGG + chr2.4:76170933-76170952 MS.gene072060:CDS 30.0%
TGAAGAAGAAGAAGAAGAAT+TGG - chr2.4:76169669-76169688 None:intergenic 30.0%
! AATTTGGTTGTTGTTGTTGT+TGG - chr2.4:76169789-76169808 None:intergenic 30.0%
! TCTGTAGGTAATTCTTTTTC+TGG + chr2.4:76170481-76170500 MS.gene072060:CDS 30.0%
AGAAGAAGAAGAAGAATTGG+AGG - chr2.4:76169666-76169685 None:intergenic 35.0%
AGAAGGTTGAATATTGGAGA+TGG - chr2.4:76169630-76169649 None:intergenic 35.0%
ATGATGAGAATTTGAAGGTG+AGG - chr2.4:76169555-76169574 None:intergenic 35.0%
CAAAACAAATCACCAACAAC+AGG + chr2.4:76170402-76170421 MS.gene072060:CDS 35.0%
CATGTTCAGTCTCAAAATCA+AGG + chr2.4:76170661-76170680 MS.gene072060:CDS 35.0%
CCATTCAATTGTTTCACCAT+CGG - chr2.4:76169978-76169997 None:intergenic 35.0%
CTTAATGCTTATACAAGAGC+TGG + chr2.4:76170871-76170890 MS.gene072060:CDS 35.0%
GAAGAAGAAGAAGAATTGGA+GGG - chr2.4:76169665-76169684 None:intergenic 35.0%
TATCAACCATCACAGCATTT+GGG + chr2.4:76170808-76170827 MS.gene072060:CDS 35.0%
! TGCTGATGATGAGAATTTGA+AGG - chr2.4:76169560-76169579 None:intergenic 35.0%
! TTGTTGTTGTTGGATACATC+TGG - chr2.4:76169779-76169798 None:intergenic 35.0%
!!! TGATTTTGAGACTGAACATG+AGG - chr2.4:76170661-76170680 None:intergenic 35.0%
AAGGCCATCACCTTTCATTA+TGG + chr2.4:76169601-76169620 MS.gene072060:CDS 40.0%
AGACACAGACACAAAACATG+TGG + chr2.4:76170626-76170645 MS.gene072060:CDS 40.0%
AGGCCATCACCTTTCATTAT+GGG + chr2.4:76169602-76169621 MS.gene072060:CDS 40.0%
CAAAACAACAAGCAGAAGAG+AGG + chr2.4:76169482-76169501 MS.gene072060:CDS 40.0%
CACCAACAACAGGATGATAT+GGG + chr2.4:76170412-76170431 MS.gene072060:CDS 40.0%
CCGATGGTGAAACAATTGAA+TGG + chr2.4:76169975-76169994 MS.gene072060:CDS 40.0%
CTCAGTTACATTTCATGCCA+AGG + chr2.4:76170707-76170726 MS.gene072060:CDS 40.0%
CTGATGAACGCTGATCAAAT+TGG + chr2.4:76170256-76170275 MS.gene072060:CDS 40.0%
GACTGAACATGAGGTGTAAA+AGG - chr2.4:76170652-76170671 None:intergenic 40.0%
GCGGTTGAAGTTGAAGAAAA+TGG - chr2.4:76169815-76169834 None:intergenic 40.0%
GCTGTATCTGATTCCAACTT+GGG + chr2.4:76170835-76170854 MS.gene072060:CDS 40.0%
GGCATGAAATGTAACTGAGA+GGG - chr2.4:76170706-76170725 None:intergenic 40.0%
GTATCAACCATCACAGCATT+TGG + chr2.4:76170807-76170826 MS.gene072060:CDS 40.0%
GTTGCCGGTAACATATTTGA+CGG - chr2.4:76170517-76170536 None:intergenic 40.0%
TCACCAACAACAGGATGATA+TGG + chr2.4:76170411-76170430 MS.gene072060:CDS 40.0%
TCGACCGTCAAATATGTTAC+CGG + chr2.4:76170510-76170529 MS.gene072060:CDS 40.0%
TCTTCTCCACTCTCATCATT+AGG - chr2.4:76170985-76171004 None:intergenic 40.0%
TGAATGGCTTCTTCAACAAG+CGG + chr2.4:76169991-76170010 MS.gene072060:CDS 40.0%
TGGCATGAAATGTAACTGAG+AGG - chr2.4:76170707-76170726 None:intergenic 40.0%
TTCCAGTTGACCAGAGAATT+AGG + chr2.4:76169944-76169963 MS.gene072060:CDS 40.0%
TTGTTGGATACATCTGGAAG+AGG - chr2.4:76169773-76169792 None:intergenic 40.0%
TTGTTTCACCATCGGATTTG+TGG - chr2.4:76169970-76169989 None:intergenic 40.0%
! GAGTGGAAGTACAATTTGGA+TGG + chr2.4:76170558-76170577 MS.gene072060:CDS 40.0%
! GTGAGGATGATTCTTGTTGT+TGG - chr2.4:76169538-76169557 None:intergenic 40.0%
!! AGGATGATTCTTGTTGTTGG+TGG - chr2.4:76169535-76169554 None:intergenic 40.0%
!! ATGATTCTTGTTGTTGGTGG+TGG - chr2.4:76169532-76169551 None:intergenic 40.0%
!! TCATCATTAGGTTGAGGTTG+AGG - chr2.4:76170973-76170992 None:intergenic 40.0%
!! TGATTCTTGTTGTTGGTGGT+GGG - chr2.4:76169531-76169550 None:intergenic 40.0%
AGCAGCAAACATACCCAAGT+TGG - chr2.4:76170851-76170870 None:intergenic 45.0%
AGGTTCAATGTTCCAGCTTC+TGG + chr2.4:76170727-76170746 MS.gene072060:CDS 45.0%
ATTTGGATGGTACCAGTGAG+TGG + chr2.4:76170571-76170590 MS.gene072060:CDS 45.0%
CAAAATGAAAGTGGCGGTGA+TGG + chr2.4:76170352-76170371 MS.gene072060:CDS 45.0%
CCTCAACCTAATGATGAGAG+TGG + chr2.4:76170976-76170995 MS.gene072060:CDS 45.0%
GAAATTGGCTGGGATTGTTC+CGG - chr2.4:76170044-76170063 None:intergenic 45.0%
GAAGCTGGAACATTGAACCT+TGG - chr2.4:76170727-76170746 None:intergenic 45.0%
GAATTAGGCCACAAATCCGA+TGG + chr2.4:76169959-76169978 MS.gene072060:CDS 45.0%
GACAGGCACACAAAAGTTGA+CGG + chr2.4:76169875-76169894 MS.gene072060:CDS 45.0%
GAGATGGAGCCCATAATGAA+AGG - chr2.4:76169614-76169633 None:intergenic 45.0%
GAGCCCATAATGAAAGGTGA+TGG - chr2.4:76169608-76169627 None:intergenic 45.0%
GAGGGAGAAGAGTTTGAAGA+AGG - chr2.4:76169647-76169666 None:intergenic 45.0%
GGACGTGGTGGTTTACAATT+GGG + chr2.4:76170778-76170797 MS.gene072060:CDS 45.0%
GGAGCTTCTTCTTCTTCTGT+AGG + chr2.4:76170466-76170485 MS.gene072060:CDS 45.0%
GGATTTGTGGCCTAATTCTC+TGG - chr2.4:76169957-76169976 None:intergenic 45.0%
GGCTGTATCTGATTCCAACT+TGG + chr2.4:76170834-76170853 MS.gene072060:CDS 45.0%
GTGATGGCCTTCCATCAAAA+GGG - chr2.4:76169592-76169611 None:intergenic 45.0%
GTTGAATTTCAAGGTGGACG+TGG + chr2.4:76170763-76170782 MS.gene072060:CDS 45.0%
TGAGGATGAAACCCTAACAG+AGG - chr2.4:76170202-76170221 None:intergenic 45.0%
TGGACGTGGTGGTTTACAAT+TGG + chr2.4:76170777-76170796 MS.gene072060:CDS 45.0%
TTCAATGTTCCAGCTTCTGG+TGG + chr2.4:76170730-76170749 MS.gene072060:CDS 45.0%
TTCTCATCATCAGCAGCTTG+TGG + chr2.4:76169565-76169584 MS.gene072060:CDS 45.0%
TTGGATACATCTGGAAGAGG+AGG - chr2.4:76169770-76169789 None:intergenic 45.0%
! AACTTTTGTGTGCCTGTCCT+TGG - chr2.4:76169873-76169892 None:intergenic 45.0%
! ACCATCACAGCATTTGGGAT+TGG + chr2.4:76170813-76170832 MS.gene072060:CDS 45.0%
! CCACTCTCATCATTAGGTTG+AGG - chr2.4:76170979-76170998 None:intergenic 45.0%
! GGTGGTGGTGTTGAATTTCA+AGG + chr2.4:76170754-76170773 MS.gene072060:CDS 45.0%
! GGTGGTGTTGAATTTCAAGG+TGG + chr2.4:76170757-76170776 MS.gene072060:CDS 45.0%
! GTTGTTGCTGTTGCTGTTGT+TGG - chr2.4:76170234-76170253 None:intergenic 45.0%
! TTGTGGTCCCTTTTGATGGA+AGG + chr2.4:76169582-76169601 MS.gene072060:CDS 45.0%
!! AGCATCAATGAGGTGTTGAG+TGG - chr2.4:76169720-76169739 None:intergenic 45.0%
!! GACGTTGAGCGTTGAGAAAT+TGG - chr2.4:76170059-76170078 None:intergenic 45.0%
!! GATTCTTGTTGTTGGTGGTG+GGG - chr2.4:76169530-76169549 None:intergenic 45.0%
!! GCATCAATGAGGTGTTGAGT+GGG - chr2.4:76169719-76169738 None:intergenic 45.0%
!! TGAGCGTTGAGAAATTGGCT+GGG - chr2.4:76170054-76170073 None:intergenic 45.0%
!! TGTTGTTGGTGGTGTTGATG+AGG - chr2.4:76170220-76170239 None:intergenic 45.0%
!! TTGAGCGTTGAGAAATTGGC+TGG - chr2.4:76170055-76170074 None:intergenic 45.0%
!! TTTTGGAGGATTTGCAACCG+CGG - chr2.4:76169843-76169862 None:intergenic 45.0%
AAGAGGAGGTTCAGATCTGG+TGG - chr2.4:76169756-76169775 None:intergenic 50.0%
AATGTTCCAGCTTCTGGTGG+TGG + chr2.4:76170733-76170752 MS.gene072060:CDS 50.0%
ACACCTCATTGATGCTTCCC+TGG + chr2.4:76169724-76169743 MS.gene072060:CDS 50.0%
ACCAACACGCTCCTCTGTTA+GGG + chr2.4:76170188-76170207 MS.gene072060:CDS 50.0%
AGATCTGGTGGCAATTGCCA+GGG - chr2.4:76169744-76169763 None:intergenic 50.0%
AGCGCGCTATGAAAACTGAG+AGG - chr2.4:76170133-76170152 None:intergenic 50.0%
CAAAACGATCCACCAAGGAC+AGG + chr2.4:76169858-76169877 MS.gene072060:CDS 50.0%
CAACAGGATGATATGGGCGA+AGG + chr2.4:76170418-76170437 MS.gene072060:CDS 50.0%
CGCCCATATCATCCTGTTGT+TGG - chr2.4:76170417-76170436 None:intergenic 50.0%
GAATTTCAAGGTGGACGTGG+TGG + chr2.4:76170766-76170785 MS.gene072060:CDS 50.0%
GATGATATGGGCGAAGGTGA+CGG + chr2.4:76170424-76170443 MS.gene072060:CDS 50.0%
GCCAATCCCAAATGCTGTGA+TGG - chr2.4:76170817-76170836 None:intergenic 50.0%
GGCCTAATTCTCTGGTCAAC+TGG - chr2.4:76169949-76169968 None:intergenic 50.0%
GGGATGGGGATGAATATTGG+TGG - chr2.4:76169510-76169529 None:intergenic 50.0%
GTGGGGATGGGGATGAATAT+TGG - chr2.4:76169513-76169532 None:intergenic 50.0%
TACCAACACGCTCCTCTGTT+AGG + chr2.4:76170187-76170206 MS.gene072060:CDS 50.0%
TCCTCCAAAACGATCCACCA+AGG + chr2.4:76169853-76169872 MS.gene072060:CDS 50.0%
TGGAAGAGGAGGTTCAGATC+TGG - chr2.4:76169759-76169778 None:intergenic 50.0%
TGGTACGCAGCTTCTTCGTA+AGG - chr2.4:76170172-76170191 None:intergenic 50.0%
! CAGCTTGTGGTCCCTTTTGA+TGG + chr2.4:76169578-76169597 MS.gene072060:CDS 50.0%
! CGGGGAGTGGAAGTACAATT+TGG + chr2.4:76170554-76170573 MS.gene072060:CDS 50.0%
! CTGAGAGGAGCTGATTTCGA+CGG - chr2.4:76170118-76170137 None:intergenic 50.0%
! GGTGATGGCCTTCCATCAAA+AGG - chr2.4:76169593-76169612 None:intergenic 50.0%
! GTTGCTGTTGCTGTTGTTGG+TGG - chr2.4:76170231-76170250 None:intergenic 50.0%
! TTTTCATAGCGCGCTTGCAC+TGG + chr2.4:76170138-76170157 MS.gene072060:CDS 50.0%
! TTTTGTGTGCCTGTCCTTGG+TGG - chr2.4:76169870-76169889 None:intergenic 50.0%
!! CTGTCCTTGGTGGATCGTTT+TGG - chr2.4:76169860-76169879 None:intergenic 50.0%
!! TCCTTGGTGGATCGTTTTGG+AGG - chr2.4:76169857-76169876 None:intergenic 50.0%
!! TTGCCAGGGAAGCATCAATG+AGG - chr2.4:76169730-76169749 None:intergenic 50.0%
!! TTGTTGTTGGTGGTGGGGAT+GGG - chr2.4:76169525-76169544 None:intergenic 50.0%
AACGTCTCACTCCGTAGCAG+CGG + chr2.4:76170073-76170092 MS.gene072060:CDS 55.0%
ACAGTGTAGAGCCGCTGCTA+CGG - chr2.4:76170087-76170106 None:intergenic 55.0%
ACTCTTGCTGCACATGTCGC+CGG - chr2.4:76169923-76169942 None:intergenic 55.0%
CACACAAAAGTTGACGGCCG+TGG + chr2.4:76169881-76169900 MS.gene072060:CDS 55.0%
CAGATCTGGTGGCAATTGCC+AGG - chr2.4:76169745-76169764 None:intergenic 55.0%
GCATTCGAATTCTCCGGCCA+CGG - chr2.4:76169901-76169920 None:intergenic 55.0%
GCTTCTTCAACAAGCGGAGC+CGG + chr2.4:76169997-76170016 MS.gene072060:CDS 55.0%
GTTACCGGCAACAGCCATGT+GGG + chr2.4:76170525-76170544 MS.gene072060:CDS 55.0%
TCGCCGGCATTCGAATTCTC+CGG - chr2.4:76169907-76169926 None:intergenic 55.0%
TGGATGGTACCAGTGAGTGG+TGG + chr2.4:76170574-76170593 MS.gene072060:CDS 55.0%
TGGCCGGAGAATTCGAATGC+CGG + chr2.4:76169901-76169920 MS.gene072060:CDS 55.0%
TGTTACCGGCAACAGCCATG+TGG + chr2.4:76170524-76170543 MS.gene072060:CDS 55.0%
! AACAGCCATGTGGGCTACAG+CGG + chr2.4:76170534-76170553 MS.gene072060:CDS 55.0%
! ACCCTAACAGAGGAGCGTGT+TGG - chr2.4:76170192-76170211 None:intergenic 55.0%
! AGAGGAGCTGATTTCGACGG+CGG - chr2.4:76170115-76170134 None:intergenic 55.0%
! GAAAGTGGCGGTGATGGTGA+TGG + chr2.4:76170358-76170377 MS.gene072060:CDS 55.0%
!! CTTGTTGTTGGTGGTGGGGA+TGG - chr2.4:76169526-76169545 None:intergenic 55.0%
!! TGTTGTTGGTGGTGGGGATG+GGG - chr2.4:76169524-76169543 None:intergenic 55.0%
AGGATTTGCAACCGCGGTGG+CGG - chr2.4:76169837-76169856 None:intergenic 60.0%
CAAAAGTTGACGGCCGTGGC+CGG + chr2.4:76169885-76169904 MS.gene072060:CDS 60.0%
GTAGCCCACATGGCTGTTGC+CGG - chr2.4:76170532-76170551 None:intergenic 60.0%
GTTGCAGCACCACCACTCAC+TGG - chr2.4:76170586-76170605 None:intergenic 60.0%
TGGAGGATTTGCAACCGCGG+TGG - chr2.4:76169840-76169859 None:intergenic 60.0%
! ACAGCCATGTGGGCTACAGC+GGG + chr2.4:76170535-76170554 MS.gene072060:CDS 60.0%
! GATATGGGCGAAGGTGACGG+TGG + chr2.4:76170427-76170446 MS.gene072060:CDS 60.0%
!! GTTCCAGCTTCTGGTGGTGG+TGG + chr2.4:76170736-76170755 MS.gene072060:CDS 60.0%
AACTTCAACCGCCGCCACCG+CGG + chr2.4:76169823-76169842 MS.gene072060:CDS 65.0%
ACTCCCCGCTGTAGCCCACA+TGG - chr2.4:76170542-76170561 None:intergenic 65.0%
ATCATCGCTGCCACCGGCAC+CGG + chr2.4:76170022-76170041 MS.gene072060:CDS 65.0%
CCACCACCACCACCAGAAGC+TGG - chr2.4:76170742-76170761 None:intergenic 65.0%
GGCTGGGATTGTTCCGGTGC+CGG - chr2.4:76170038-76170057 None:intergenic 65.0%
! ATGGGCGAAGGTGACGGTGG+TGG + chr2.4:76170430-76170449 MS.gene072060:CDS 65.0%
! ATGTGGGCTACAGCGGGGAG+TGG + chr2.4:76170541-76170560 MS.gene072060:CDS 65.0%
! ATTTGCAACCGCGGTGGCGG+CGG - chr2.4:76169834-76169853 None:intergenic 65.0%
! CAGCCATGTGGGCTACAGCG+GGG + chr2.4:76170536-76170555 MS.gene072060:CDS 65.0%
! TGGGATTGTTCCGGTGCCGG+TGG - chr2.4:76170035-76170054 None:intergenic 65.0%
!! CCAGCTTCTGGTGGTGGTGG+TGG + chr2.4:76170739-76170758 MS.gene072060:CDS 65.0%
!! GAAGGTGACGGTGGTGGAGG+AGG + chr2.4:76170436-76170455 MS.gene072060:CDS 65.0%
CCGGCGATCATCGCTGCCAC+CGG + chr2.4:76170016-76170035 MS.gene072060:CDS 70.0%
CCGGTGGCAGCGATGATCGC+CGG - chr2.4:76170019-76170038 None:intergenic 70.0%
! GGCGAAGGTGACGGTGGTGG+AGG + chr2.4:76170433-76170452 MS.gene072060:CDS 70.0%
!! GACGGTGGTGGAGGAGGTGG+TGG + chr2.4:76170442-76170461 MS.gene072060:CDS 70.0%
!! GGTGACGGTGGTGGAGGAGG+TGG + chr2.4:76170439-76170458 MS.gene072060:CDS 70.0%
!! GGTGGTGGAGGAGGTGGTGG+TGG + chr2.4:76170445-76170464 MS.gene072060:CDS 70.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 76169464 76171026 76169464 ID=MS.gene072060
chr2.4 mRNA 76169464 76171026 76169464 ID=MS.gene072060.t1;Parent=MS.gene072060
chr2.4 exon 76169464 76171026 76169464 ID=MS.gene072060.t1.exon1;Parent=MS.gene072060.t1
chr2.4 CDS 76169464 76171026 76169464 ID=cds.MS.gene072060.t1;Parent=MS.gene072060.t1
Gene Sequence

>MS.gene072060

ATGGAACTATCAGATTTACAAAACAACAAGCAGAAGAGAGGCACCACCAATATTCATCCCCATCCCCACCACCAACAACAAGAATCATCCTCACCTTCAAATTCTCATCATCAGCAGCTTGTGGTCCCTTTTGATGGAAGGCCATCACCTTTCATTATGGGCTCCATCTCCAATATTCAACCTTCTTCAAACTCTTCTCCCTCCAATTCTTCTTCTTCTTCTTCAGCTTCAGCTTCAGCTTCAGCTTCTGCACCCACTCAACACCTCATTGATGCTTCCCTGGCAATTGCCACCAGATCTGAACCTCCTCTTCCAGATGTATCCAACAACAACAACAACCAAATTCAACCATTTTCTTCAACTTCAACCGCCGCCACCGCGGTTGCAAATCCTCCAAAACGATCCACCAAGGACAGGCACACAAAAGTTGACGGCCGTGGCCGGAGAATTCGAATGCCGGCGACATGTGCAGCAAGAGTTTTCCAGTTGACCAGAGAATTAGGCCACAAATCCGATGGTGAAACAATTGAATGGCTTCTTCAACAAGCGGAGCCGGCGATCATCGCTGCCACCGGCACCGGAACAATCCCAGCCAATTTCTCAACGCTCAACGTCTCACTCCGTAGCAGCGGCTCTACACTGTCTGCGCCGCCGTCGAAATCAGCTCCTCTCAGTTTTCATAGCGCGCTTGCACTGGCTCATCATCCTTACGAAGAAGCTGCGTACCAACACGCTCCTCTGTTAGGGTTTCATCCTCATCAACACCACCAACAACAGCAACAGCAACAACAACTGATGAACGCTGATCAAATTGGTGAAGCACTTTCAAACGAGAATTACATGAGAAAACGGTATAGAGAAGATCTTTTCAAAGATGATAACAACAATCAAAATGAAAGTGGCGGTGATGGTGATGGTTCTTCTTCTCCCAAAAGTTTCAAAACAAATCACCAACAACAGGATGATATGGGCGAAGGTGACGGTGGTGGAGGAGGTGGTGGTGGAGCTTCTTCTTCTTCTGTAGGTAATTCTTTTTCTGGACTTCTTCGACCGTCAAATATGTTACCGGCAACAGCCATGTGGGCTACAGCGGGGAGTGGAAGTACAATTTGGATGGTACCAGTGAGTGGTGGTGCTGCAACTTCTTCAAATTCAGAGACACAGACACAGACACAAAACATGTGGCCTTTTACACCTCATGTTCAGTCTCAAAATCAAGGCAACGCTGCTGCTGTAACTCCCTCTCAGTTACATTTCATGCCAAGGTTCAATGTTCCAGCTTCTGGTGGTGGTGGTGGTGTTGAATTTCAAGGTGGACGTGGTGGTTTACAATTGGGTTCAGCGTATCAACCATCACAGCATTTGGGATTGGCTGTATCTGATTCCAACTTGGGTATGTTTGCTGCTCTTAATGCTTATACAAGAGCTGGCCATTTCAACGTTAACAACTCTTCTGATCATCATCATCATCAACAACATTCAATGTCATTGGACTCTCATTCTCAACCTCAACCTCAACCTAATGATGAGAGTGGAGAAGATGCTCCTAATACCTCACAATGA
Protein sequence

>MS.gene072060.t1

MELSDLQNNKQKRGTTNIHPHPHHQQQESSSPSNSHHQQLVVPFDGRPSPFIMGSISNIQPSSNSSPSNSSSSSSASASASASAPTQHLIDASLAIATRSEPPLPDVSNNNNNQIQPFSSTSTAATAVANPPKRSTKDRHTKVDGRGRRIRMPATCAARVFQLTRELGHKSDGETIEWLLQQAEPAIIAATGTGTIPANFSTLNVSLRSSGSTLSAPPSKSAPLSFHSALALAHHPYEEAAYQHAPLLGFHPHQHHQQQQQQQQLMNADQIGEALSNENYMRKRYREDLFKDDNNNQNESGGDGDGSSSPKSFKTNHQQQDDMGEGDGGGGGGGGASSSSVGNSFSGLLRPSNMLPATAMWATAGSGSTIWMVPVSGGAATSSNSETQTQTQNMWPFTPHVQSQNQGNAAAVTPSQLHFMPRFNVPASGGGGGVEFQGGRGGLQLGSAYQPSQHLGLAVSDSNLGMFAALNAYTRAGHFNVNNSSDHHHHQQHSMSLDSHSQPQPQPNDESGEDAPNTSQ