AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene072695

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072695.t1 MTR_7g109580 91.087 561 40 3 1 556 1 556 0.0 1047
MS.gene072695.t1 MTR_1g086970 58.212 481 179 9 89 556 9 480 0.0 528
MS.gene072695.t1 MTR_2g034260 43.561 528 276 8 41 556 69 586 2.88e-137 410
MS.gene072695.t1 MTR_2g034250 45.570 474 237 6 104 556 114 587 3.13e-131 395
MS.gene072695.t1 MTR_2g034280 41.399 529 266 6 71 556 50 577 7.88e-130 391
MS.gene072695.t1 MTR_3g065980 27.848 395 258 5 176 555 159 541 1.60e-44 166
MS.gene072695.t1 MTR_7g027190 29.975 397 252 10 175 554 286 673 1.56e-42 162
MS.gene072695.t1 MTR_7g074650 32.240 366 227 9 194 554 443 792 8.71e-41 158
MS.gene072695.t1 MTR_7g062120 29.843 382 249 9 184 554 367 740 2.12e-38 150
MS.gene072695.t1 MTR_4g104020 27.475 404 263 12 171 554 127 520 1.67e-36 142
MS.gene072695.t1 MTR_1g069725 28.232 379 253 9 179 553 99 462 2.05e-34 136
MS.gene072695.t1 MTR_2g026250 27.666 347 235 8 217 554 257 596 2.63e-34 137
MS.gene072695.t1 MTR_4g102790 28.205 351 231 10 216 553 319 661 7.08e-34 137
MS.gene072695.t1 MTR_5g094450 27.273 385 256 10 185 554 157 532 2.56e-33 134
MS.gene072695.t1 MTR_4g064200 27.604 384 251 12 184 553 279 649 3.33e-33 134
MS.gene072695.t1 MTR_4g077760 30.081 369 239 11 194 554 198 555 1.96e-32 131
MS.gene072695.t1 MTR_2g097310 26.984 378 261 9 184 553 267 637 3.62e-32 131
MS.gene072695.t1 MTR_2g097410 28.982 383 251 11 184 553 366 740 4.92e-32 131
MS.gene072695.t1 MTR_4g064160 26.509 381 261 8 184 553 311 683 7.55e-31 127
MS.gene072695.t1 MTR_4g076140 26.735 490 266 16 137 554 1 469 1.16e-30 125
MS.gene072695.t1 MTR_2g097467 26.385 379 261 8 184 553 284 653 1.47e-30 126
MS.gene072695.t1 MTR_3g056110 25.197 381 265 9 185 554 171 542 1.67e-30 125
MS.gene072695.t1 MTR_2g097463 26.110 383 257 8 184 553 285 654 1.89e-30 126
MS.gene072695.t1 MTR_2g097473 26.371 383 256 8 184 553 284 653 2.09e-30 126
MS.gene072695.t1 MTR_4g076020 28.689 366 231 9 194 554 89 429 2.91e-30 123
MS.gene072695.t1 MTR_4g133660 25.434 346 242 8 217 553 204 542 6.23e-30 124
MS.gene072695.t1 MTR_3g089055 27.704 379 252 10 188 554 197 565 2.92e-29 122
MS.gene072695.t1 MTR_3g089055 27.704 379 252 10 188 554 202 570 3.46e-29 122
MS.gene072695.t1 MTR_4g064150 26.410 390 264 10 179 553 351 732 3.81e-29 122
MS.gene072695.t1 MTR_4g064120 25.648 386 266 9 178 549 243 621 4.14e-29 122
MS.gene072695.t1 MTR_2g097390 25.000 380 266 9 184 553 270 640 1.28e-28 120
MS.gene072695.t1 MTR_2g082090 25.792 442 281 15 145 553 151 578 1.34e-28 120
MS.gene072695.t1 MTR_8g442410 26.735 389 260 12 181 554 147 525 4.89e-28 118
MS.gene072695.t1 MTR_2g097350 26.509 381 259 10 184 553 269 639 1.00e-26 115
MS.gene072695.t1 MTR_2g097380 24.863 366 235 9 194 553 229 560 2.10e-26 113
MS.gene072695.t1 MTR_4g064180 24.543 383 274 8 178 549 243 621 2.83e-26 113
MS.gene072695.t1 MTR_5g097480 24.176 364 249 10 209 554 190 544 5.16e-26 112
MS.gene072695.t1 MTR_3g072710 26.396 394 267 13 179 553 110 499 2.81e-25 109
MS.gene072695.t1 MTR_4g097080 24.372 398 282 8 166 554 116 503 3.66e-25 109
MS.gene072695.t1 MTR_7g057230 27.799 259 179 3 299 553 402 656 5.68e-25 109
MS.gene072695.t1 MTR_1g096030 25.721 416 253 15 176 556 67 461 8.22e-25 107
MS.gene072695.t1 MTR_3g022830 23.923 418 252 9 194 555 30 437 8.57e-25 107
MS.gene072695.t1 MTR_1g029420 27.177 379 254 12 188 554 224 592 1.73e-24 108
MS.gene072695.t1 MTR_5g058860 24.250 400 268 13 178 553 108 496 1.02e-21 99.0
MS.gene072695.t1 MTR_0092s0100 27.273 374 245 11 190 554 373 728 1.96e-19 92.8
MS.gene072695.t1 MTR_0092s0100 27.273 374 245 11 190 554 376 731 2.00e-19 92.8
MS.gene072695.t1 MTR_4g026485 24.661 369 260 8 190 554 270 624 2.79e-19 92.0
MS.gene072695.t1 MTR_5g015490 24.242 363 262 5 204 554 129 490 8.96e-19 89.7
MS.gene072695.t1 MTR_6g047750 27.306 271 173 7 299 554 401 662 3.59e-18 88.6
MS.gene072695.t1 MTR_8g093070 29.932 294 175 14 274 553 219 495 3.79e-18 87.8
MS.gene072695.t1 MTR_5g009080 26.923 260 183 4 140 399 8 260 6.68e-15 77.8
MS.gene072695.t1 MTR_5g009080 30.573 157 97 6 404 554 328 478 1.94e-13 73.2
MS.gene072695.t1 MTR_8g077940 23.011 352 236 10 217 554 211 541 6.67e-13 71.6
MS.gene072695.t1 MTR_5g015950 22.910 323 200 10 274 553 129 445 1.40e-12 70.5
MS.gene072695.t1 MTR_3g053270 22.910 323 200 10 274 553 129 445 1.40e-12 70.5
MS.gene072695.t1 MTR_2g099110 23.464 358 248 11 216 554 101 451 6.00e-12 68.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene072695.t1 AT3G03450 28.682 387 259 6 176 555 170 546 1.72e-44 166
MS.gene072695.t1 AT2G01570 27.979 386 258 7 176 555 211 582 1.04e-43 164
MS.gene072695.t1 AT1G14920 28.125 384 260 6 176 555 159 530 1.98e-43 162
MS.gene072695.t1 AT1G66350 28.418 373 251 6 184 555 150 507 6.02e-43 160
MS.gene072695.t1 AT5G17490 28.232 379 257 5 180 555 151 517 1.54e-41 157
MS.gene072695.t1 AT3G54220 31.507 365 232 9 194 553 298 649 1.16e-38 150
MS.gene072695.t1 AT3G46600 29.261 352 227 9 215 554 30 371 2.48e-38 145
MS.gene072695.t1 AT3G46600 28.021 389 258 9 178 554 201 579 2.91e-38 149
MS.gene072695.t1 AT3G46600 28.021 389 258 9 178 554 169 547 3.75e-38 148
MS.gene072695.t1 AT5G41920 31.398 379 236 12 184 554 38 400 3.99e-38 145
MS.gene072695.t1 AT2G29065 28.205 390 251 9 184 553 247 627 1.12e-37 147
MS.gene072695.t1 AT5G59450 25.455 385 265 8 184 554 222 598 4.88e-34 137
MS.gene072695.t1 AT1G07530 27.154 383 255 9 185 553 392 764 3.00e-33 135
MS.gene072695.t1 AT2G29060 28.534 382 253 10 185 553 317 691 6.63e-33 134
MS.gene072695.t1 AT2G29060 28.534 382 253 10 185 553 280 654 6.73e-33 134
MS.gene072695.t1 AT1G07520 26.368 402 260 12 175 553 140 528 1.54e-32 131
MS.gene072695.t1 AT1G07520 26.368 402 260 12 175 553 140 528 1.54e-32 131
MS.gene072695.t1 AT1G07520 26.368 402 260 12 175 553 304 692 1.99e-32 132
MS.gene072695.t1 AT1G07520 26.368 402 260 12 175 553 305 693 2.11e-32 132
MS.gene072695.t1 AT5G66770 28.718 390 255 10 177 554 206 584 7.51e-32 130
MS.gene072695.t1 AT2G37650 26.440 382 260 8 184 554 330 701 1.17e-31 130
MS.gene072695.t1 AT2G37650 26.440 382 260 8 184 554 342 713 1.60e-31 130
MS.gene072695.t1 AT4G17230 25.131 382 266 8 184 554 153 525 2.11e-31 128
MS.gene072695.t1 AT4G17230 25.131 382 266 8 184 554 153 525 2.11e-31 128
MS.gene072695.t1 AT1G63100 26.012 346 237 7 223 553 312 653 2.22e-30 126
MS.gene072695.t1 AT1G63100 26.012 346 237 7 223 553 287 628 2.44e-30 126
MS.gene072695.t1 AT2G04890 25.867 375 263 8 185 554 49 413 4.66e-29 119
MS.gene072695.t1 AT1G55580 26.290 407 260 9 186 554 41 445 3.17e-27 115
MS.gene072695.t1 AT3G50650 27.013 385 256 11 183 554 170 542 4.62e-27 115
MS.gene072695.t1 AT1G50600 25.065 383 263 10 185 554 155 526 6.63e-27 115
MS.gene072695.t1 AT1G50600 25.065 383 263 10 185 554 226 597 1.15e-26 114
MS.gene072695.t1 AT1G50600 25.065 383 263 10 185 554 230 601 1.20e-26 114
MS.gene072695.t1 AT1G50420 23.669 507 286 15 117 554 5 479 8.74e-26 111
MS.gene072695.t1 AT5G48150 23.647 351 245 9 217 554 150 490 1.81e-24 107
MS.gene072695.t1 AT5G48150 23.647 351 245 9 217 554 150 490 1.81e-24 107
MS.gene072695.t1 AT5G48150 23.647 351 245 9 217 554 150 490 1.81e-24 107
MS.gene072695.t1 AT5G48150 23.647 351 245 9 217 554 150 490 1.81e-24 107
MS.gene072695.t1 AT4G08250 24.876 402 253 15 179 553 97 476 6.61e-24 105
MS.gene072695.t1 AT4G37650 22.871 411 293 6 162 554 125 529 5.00e-20 94.0
MS.gene072695.t1 AT4G36710 24.877 406 268 14 165 555 103 486 1.36e-19 92.4
MS.gene072695.t1 AT1G21450 24.606 317 225 7 188 495 224 535 4.18e-17 85.1
MS.gene072695.t1 AT3G49950 24.650 357 236 9 220 553 61 407 5.76e-16 80.5
MS.gene072695.t1 AT5G52510 23.551 276 188 7 298 554 369 640 5.43e-14 75.5
MS.gene072695.t1 AT2G45160 23.547 344 231 13 224 554 315 639 3.56e-12 69.7
MS.gene072695.t1 AT3G60630 24.834 302 189 13 274 554 338 622 4.46e-12 69.3

Find 69 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TACTAAAGCTTCTTCAAATT+TGG 0.128771 7.3:+5424219 MS.gene072695:CDS
CCTTATAGATTTGACCTTAA+TGG 0.142996 7.3:+5424349 MS.gene072695:CDS
TAAAACAGGTTTATATAAAA+AGG 0.190685 7.3:-5424923 None:intergenic
AATTGCATAACTTGATTAAA+AGG 0.206067 7.3:-5424667 None:intergenic
AATCAAGTTATGCAATTTAC+TGG 0.238145 7.3:+5424673 MS.gene072695:CDS
GAATGGGAAGTGTTTGATAT+TGG 0.247092 7.3:+5425446 MS.gene072695:CDS
ATTAAAGTCCTTAGAAAATA+TGG 0.247192 7.3:-5425018 None:intergenic
CTCAATATTCTTCTTCTCAT+TGG 0.265071 7.3:+5424299 MS.gene072695:CDS
AATATCAAGGCAGAATTCTA+TGG 0.265930 7.3:+5425038 MS.gene072695:CDS
CAGCTTGGAGGTTTCTTTGA+TGG 0.281090 7.3:+5425499 MS.gene072695:CDS
GTTGTTGTTGATCATGATTT+TGG 0.285842 7.3:-5424000 None:intergenic
CCATTAAGGTCAAATCTATA+AGG 0.308445 7.3:-5424349 None:intergenic
GAAACAGGAAGAGTGATTAA+AGG 0.316278 7.3:+5424568 MS.gene072695:CDS
CTTCCTCAGATAAACCATTA+AGG 0.338720 7.3:-5424363 None:intergenic
AACATATCTTCTACTAAAAC+AGG 0.348026 7.3:-5424937 None:intergenic
ATGATTTCTCAGAATCTCTA+AGG 0.348847 7.3:-5424164 None:intergenic
GGTAGTTGTTCATTCTCATT+TGG 0.361963 7.3:-5423962 None:intergenic
CAAATCTATAAGGGTGTGTT+TGG 0.366485 7.3:-5424339 None:intergenic
GAAATGTGAAAATTGATGTT+TGG 0.381287 7.3:+5425307 MS.gene072695:CDS
AAGAACAAAATTGAAGAAAC+TGG 0.386217 7.3:+5424862 MS.gene072695:CDS
AATGGGAAGTGTTTGATATT+GGG 0.407459 7.3:+5425447 MS.gene072695:CDS
GAGAGAATAGAGAAAGAAAC+AGG 0.418963 7.3:+5424553 MS.gene072695:CDS
GAAGGAATAATAAGAGTAGC+TGG 0.427571 7.3:+5424265 MS.gene072695:CDS
TAGAAAATATGGAGAATAAA+CGG 0.437645 7.3:-5425007 None:intergenic
TTCTAAGGACTTTAATATCA+AGG 0.440021 7.3:+5425025 MS.gene072695:CDS
AAACAGGAAGAGTGATTAAA+GGG 0.451323 7.3:+5424569 MS.gene072695:CDS
TTTGCGATGTGGAAAAGAAT+GGG 0.457035 7.3:+5425430 MS.gene072695:CDS
GCAAGCACTTGCTGAGAGAC+AGG 0.457628 7.3:+5424789 MS.gene072695:CDS
TTAGAGATTCTGAGAAATCA+TGG 0.458237 7.3:+5424166 MS.gene072695:CDS
GTGTCTAAAGAGTTTGATCA+TGG 0.459768 7.3:+5425402 MS.gene072695:CDS
CATTAAGGTCAAATCTATAA+GGG 0.469089 7.3:-5424348 None:intergenic
GGTATAGAATGGTGGAAACT+AGG 0.481546 7.3:+5425346 MS.gene072695:CDS
ATTCTTTGCAAGGTATAGAA+TGG 0.481831 7.3:+5425335 MS.gene072695:CDS
ATGTGTTTACCAAGCTGAAT+TGG 0.482417 7.3:+5425380 MS.gene072695:CDS
CAATGGAATTCTTCCATCAC+AGG 0.487853 7.3:+5424484 MS.gene072695:CDS
TGAAGCGTTGTTCTTCTACT+CGG 0.492367 7.3:+5425161 MS.gene072695:CDS
ACAGAAGGAATATTATCTGC+AGG 0.492833 7.3:+5425240 MS.gene072695:CDS
AAGTGAGTTACTTGACAAGA+TGG 0.495737 7.3:+5424609 MS.gene072695:CDS
TTGAACACAATTTCCTGTGA+TGG 0.496409 7.3:-5424497 None:intergenic
TGTATTCAATCTCAGCTTGG+AGG 0.499895 7.3:+5425487 MS.gene072695:CDS
AGAAATATTGTCGCGTCAGA+AGG 0.509809 7.3:+5425267 MS.gene072695:CDS
CAACAACAAGAATTCGAAGT+TGG 0.519349 7.3:+5424016 MS.gene072695:CDS
TGTTCATTCTCATTTGGAAG+AGG 0.520704 7.3:-5423956 None:intergenic
CTTTAGACACCAATTCAGCT+TGG 0.526186 7.3:-5425389 None:intergenic
GTGTTTGATATTGGGATGGA+AGG 0.529103 7.3:+5425455 MS.gene072695:CDS
TGTTTGATATTGGGATGGAA+GGG 0.535874 7.3:+5425456 MS.gene072695:CDS
AGAACAACGCTTCAACGAAT+CGG 0.538399 7.3:-5425152 None:intergenic
TCAAGTACTATCATTATACA+TGG 0.539208 7.3:-5425093 None:intergenic
TTTGGAGAAGATTCTTTGCA+AGG 0.540946 7.3:+5425325 MS.gene072695:CDS
TCTAAGGATGATAAATATTG+TGG 0.555614 7.3:-5424148 None:intergenic
CTTGCATCAAAGCTATGCAC+TGG 0.559445 7.3:-5424771 None:intergenic
GTTTGATATTGGGATGGAAG+GGG 0.560675 7.3:+5425457 MS.gene072695:CDS
CAATGTATTCAATCTCAGCT+TGG 0.567255 7.3:+5425484 MS.gene072695:CDS
CAAGCTGAGATTGAATACAT+TGG 0.568796 7.3:-5425483 None:intergenic
GGAAGTGTTTGATATTGGGA+TGG 0.581535 7.3:+5425451 MS.gene072695:CDS
AGTGAGTTACTTGACAAGAT+GGG 0.584301 7.3:+5424610 MS.gene072695:CDS
TCGCGTCAGAAGGTAGAGAA+AGG 0.588622 7.3:+5425277 MS.gene072695:CDS
CATCAAAAGATGTCAACAGA+AGG 0.596462 7.3:+5424247 MS.gene072695:CDS
CAGAAGGAATATTATCTGCA+GGG 0.601009 7.3:+5425241 MS.gene072695:CDS
TGCTGAGAGACAGGATTGCA+TGG 0.602620 7.3:+5424798 MS.gene072695:CDS
CTTTGCAAGGTATAGAATGG+TGG 0.606333 7.3:+5425338 MS.gene072695:CDS
TGACCTTAATGGTTTATCTG+AGG 0.606438 7.3:+5424360 MS.gene072695:CDS
GAGTGTAGGATGATAACAGA+AGG 0.608979 7.3:+5425225 MS.gene072695:CDS
TTCTTCTACAAAGTCATGAG+TGG 0.609126 7.3:-5423870 None:intergenic
TAATAAGAGTAGCTGGAGCA+AGG 0.609556 7.3:+5424272 MS.gene072695:CDS
TGAGCGAGGAGGATGAGTGT+AGG 0.628200 7.3:+5425211 MS.gene072695:CDS
TGTCTAAAGAGTTTGATCAT+GGG 0.633104 7.3:+5425403 MS.gene072695:CDS
GGCTGAAACATGTATGAGCG+AGG 0.648686 7.3:+5425197 MS.gene072695:CDS
TGAAACATGTATGAGCGAGG+AGG 0.773647 7.3:+5425200 MS.gene072695:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAACAGGTTTATATAAAAA+GGG - chr7.3:5424925-5424944 None:intergenic 15.0%
!! TAAAACAGGTTTATATAAAA+AGG - chr7.3:5424926-5424945 None:intergenic 15.0%
!!! AATTACTTTTATATTGTCAA+TGG + chr7.3:5424467-5424486 MS.gene072695:CDS 15.0%
!! AATTGCATAACTTGATTAAA+AGG - chr7.3:5424670-5424689 None:intergenic 20.0%
!! ATTAAAGTCCTTAGAAAATA+TGG - chr7.3:5425021-5425040 None:intergenic 20.0%
!! TAGAAAATATGGAGAATAAA+CGG - chr7.3:5425010-5425029 None:intergenic 20.0%
!!! AGATGATTTTGAAATTGAAA+AGG + chr7.3:5424972-5424991 MS.gene072695:CDS 20.0%
!!! ATAGATTTTGATATAAGAAG+TGG + chr7.3:5424745-5424764 MS.gene072695:CDS 20.0%
!!! ATATTGTTTTCTAAAGGTTT+TGG - chr7.3:5424118-5424137 None:intergenic 20.0%
!!! TAGATTTTGATATAAGAAGT+GGG + chr7.3:5424746-5424765 MS.gene072695:CDS 20.0%
!!! TATTTTGTTGATTAGTTTCT+AGG - chr7.3:5424072-5424091 None:intergenic 20.0%
!!! TTCTGAATATTGTTTTCTAA+AGG - chr7.3:5424124-5424143 None:intergenic 20.0%
!!! TTTATTCTCCATATTTTCTA+AGG + chr7.3:5425010-5425029 MS.gene072695:CDS 20.0%
! AACATATCTTCTACTAAAAC+AGG - chr7.3:5424940-5424959 None:intergenic 25.0%
! AAGAACAAAATTGAAGAAAC+TGG + chr7.3:5424862-5424881 MS.gene072695:CDS 25.0%
! AATCAAGTTATGCAATTTAC+TGG + chr7.3:5424673-5424692 MS.gene072695:CDS 25.0%
! CATTAAGGTCAAATCTATAA+GGG - chr7.3:5424351-5424370 None:intergenic 25.0%
! GAAATGTGAAAATTGATGTT+TGG + chr7.3:5425307-5425326 MS.gene072695:CDS 25.0%
! TACTAAAGCTTCTTCAAATT+TGG + chr7.3:5424219-5424238 MS.gene072695:CDS 25.0%
! TCAAGTACTATCATTATACA+TGG - chr7.3:5425096-5425115 None:intergenic 25.0%
! TCTAAGGATGATAAATATTG+TGG - chr7.3:5424151-5424170 None:intergenic 25.0%
!! AAAAACTATGTTGTTTGTCT+TGG - chr7.3:5423904-5423923 None:intergenic 25.0%
!! TTCTAAGGACTTTAATATCA+AGG + chr7.3:5425025-5425044 MS.gene072695:CDS 25.0%
!! TTGCATGGTTGAAATTTTTA+AGG + chr7.3:5424813-5424832 MS.gene072695:CDS 25.0%
!!! AGATTTTGATATAAGAAGTG+GGG + chr7.3:5424747-5424766 MS.gene072695:CDS 25.0%
!!! ATTTTTAAGGTAAGTGCTAT+TGG + chr7.3:5424826-5424845 MS.gene072695:CDS 25.0%
!!! TGTTTTAGTAGAAGATATGT+TGG + chr7.3:5424939-5424958 MS.gene072695:CDS 25.0%
!!! TTTTCTAAAGGTTTTGGAAT+TGG - chr7.3:5424112-5424131 None:intergenic 25.0%
!!! TTTTTCTGGAATACAATTGT+TGG + chr7.3:5423919-5423938 MS.gene072695:CDS 25.0%
AAACAGGAAGAGTGATTAAA+GGG + chr7.3:5424569-5424588 MS.gene072695:CDS 30.0%
AATATCAAGGCAGAATTCTA+TGG + chr7.3:5425038-5425057 MS.gene072695:CDS 30.0%
ATGATTTCTCAGAATCTCTA+AGG - chr7.3:5424167-5424186 None:intergenic 30.0%
ATTCTTTGCAAGGTATAGAA+TGG + chr7.3:5425335-5425354 MS.gene072695:CDS 30.0%
CCATTAAGGTCAAATCTATA+AGG - chr7.3:5424352-5424371 None:intergenic 30.0%
CCTTATAGATTTGACCTTAA+TGG + chr7.3:5424349-5424368 MS.gene072695:CDS 30.0%
CTCAATATTCTTCTTCTCAT+TGG + chr7.3:5424299-5424318 MS.gene072695:CDS 30.0%
TGTCTAAAGAGTTTGATCAT+GGG + chr7.3:5425403-5425422 MS.gene072695:CDS 30.0%
! GACAAACAACATAGTTTTTC+TGG + chr7.3:5423905-5423924 MS.gene072695:CDS 30.0%
! TTAGAGATTCTGAGAAATCA+TGG + chr7.3:5424166-5424185 MS.gene072695:CDS 30.0%
!! AATGGGAAGTGTTTGATATT+GGG + chr7.3:5425447-5425466 MS.gene072695:CDS 30.0%
!! GTTGTTGTTGATCATGATTT+TGG - chr7.3:5424003-5424022 None:intergenic 30.0%
!! TTTTGGTTGTTGAACATGTT+GGG - chr7.3:5423986-5424005 None:intergenic 30.0%
!!! ATTTTGGTTGTTGAACATGT+TGG - chr7.3:5423987-5424006 None:intergenic 30.0%
AAGTGAGTTACTTGACAAGA+TGG + chr7.3:5424609-5424628 MS.gene072695:CDS 35.0%
ACAGAAGGAATATTATCTGC+AGG + chr7.3:5425240-5425259 MS.gene072695:CDS 35.0%
AGTCATGAGTGGAAGAAAAA+TGG - chr7.3:5423862-5423881 None:intergenic 35.0%
AGTGAGTTACTTGACAAGAT+GGG + chr7.3:5424610-5424629 MS.gene072695:CDS 35.0%
ATGTGTTTACCAAGCTGAAT+TGG + chr7.3:5425380-5425399 MS.gene072695:CDS 35.0%
CAACAACAAGAATTCGAAGT+TGG + chr7.3:5424016-5424035 MS.gene072695:CDS 35.0%
CAAGCTGAGATTGAATACAT+TGG - chr7.3:5425486-5425505 None:intergenic 35.0%
CAATGTATTCAATCTCAGCT+TGG + chr7.3:5425484-5425503 MS.gene072695:CDS 35.0%
CAGAAGGAATATTATCTGCA+GGG + chr7.3:5425241-5425260 MS.gene072695:CDS 35.0%
CATCAAAAGATGTCAACAGA+AGG + chr7.3:5424247-5424266 MS.gene072695:CDS 35.0%
CTTCCTCAGATAAACCATTA+AGG - chr7.3:5424366-5424385 None:intergenic 35.0%
GAAACAGGAAGAGTGATTAA+AGG + chr7.3:5424568-5424587 MS.gene072695:CDS 35.0%
GAAGGAATAATAAGAGTAGC+TGG + chr7.3:5424265-5424284 MS.gene072695:CDS 35.0%
GAGAGAATAGAGAAAGAAAC+AGG + chr7.3:5424553-5424572 MS.gene072695:CDS 35.0%
GGTAGTTGTTCATTCTCATT+TGG - chr7.3:5423965-5423984 None:intergenic 35.0%
GTGTCTAAAGAGTTTGATCA+TGG + chr7.3:5425402-5425421 MS.gene072695:CDS 35.0%
TGACCTTAATGGTTTATCTG+AGG + chr7.3:5424360-5424379 MS.gene072695:CDS 35.0%
TGTTCATTCTCATTTGGAAG+AGG - chr7.3:5423959-5423978 None:intergenic 35.0%
TGTTTGATATTGGGATGGAA+GGG + chr7.3:5425456-5425475 MS.gene072695:CDS 35.0%
TTCTTCTACAAAGTCATGAG+TGG - chr7.3:5423873-5423892 None:intergenic 35.0%
TTGAACACAATTTCCTGTGA+TGG - chr7.3:5424500-5424519 None:intergenic 35.0%
TTTGCGATGTGGAAAAGAAT+GGG + chr7.3:5425430-5425449 MS.gene072695:CDS 35.0%
TTTGGAGAAGATTCTTTGCA+AGG + chr7.3:5425325-5425344 MS.gene072695:CDS 35.0%
! CAAATCTATAAGGGTGTGTT+TGG - chr7.3:5424342-5424361 None:intergenic 35.0%
! TTTTGCGATGTGGAAAAGAA+TGG + chr7.3:5425429-5425448 MS.gene072695:CDS 35.0%
!! GAATGGGAAGTGTTTGATAT+TGG + chr7.3:5425446-5425465 MS.gene072695:CDS 35.0%
!!! GTTTTGGAATTGGAACTTTG+TGG - chr7.3:5424102-5424121 None:intergenic 35.0%
AGAAATATTGTCGCGTCAGA+AGG + chr7.3:5425267-5425286 MS.gene072695:CDS 40.0%
AGAACAACGCTTCAACGAAT+CGG - chr7.3:5425155-5425174 None:intergenic 40.0%
CAATGGAATTCTTCCATCAC+AGG + chr7.3:5424484-5424503 MS.gene072695:CDS 40.0%
CTTTAGACACCAATTCAGCT+TGG - chr7.3:5425392-5425411 None:intergenic 40.0%
CTTTGCAAGGTATAGAATGG+TGG + chr7.3:5425338-5425357 MS.gene072695:CDS 40.0%
GAGTGTAGGATGATAACAGA+AGG + chr7.3:5425225-5425244 MS.gene072695:CDS 40.0%
GTGTTTGATATTGGGATGGA+AGG + chr7.3:5425455-5425474 MS.gene072695:CDS 40.0%
GTTTGATATTGGGATGGAAG+GGG + chr7.3:5425457-5425476 MS.gene072695:CDS 40.0%
TAATAAGAGTAGCTGGAGCA+AGG + chr7.3:5424272-5424291 MS.gene072695:CDS 40.0%
TGTATTCAATCTCAGCTTGG+AGG + chr7.3:5425487-5425506 MS.gene072695:CDS 40.0%
! GGTATAGAATGGTGGAAACT+AGG + chr7.3:5425346-5425365 MS.gene072695:CDS 40.0%
! TCATGGGAAATTTTGCGATG+TGG + chr7.3:5425419-5425438 MS.gene072695:CDS 40.0%
!! GGAAGTGTTTGATATTGGGA+TGG + chr7.3:5425451-5425470 MS.gene072695:CDS 40.0%
!! TGAAGCGTTGTTCTTCTACT+CGG + chr7.3:5425161-5425180 MS.gene072695:CDS 40.0%
!!! CTACTCGGCTTTTTTCGATA+TGG + chr7.3:5425176-5425195 MS.gene072695:CDS 40.0%
CTTGCATCAAAGCTATGCAC+TGG - chr7.3:5424774-5424793 None:intergenic 45.0%
TGAAACATGTATGAGCGAGG+AGG + chr7.3:5425200-5425219 MS.gene072695:CDS 45.0%
GGCTGAAACATGTATGAGCG+AGG + chr7.3:5425197-5425216 MS.gene072695:CDS 50.0%
TCGCGTCAGAAGGTAGAGAA+AGG + chr7.3:5425277-5425296 MS.gene072695:CDS 50.0%
TGCTGAGAGACAGGATTGCA+TGG + chr7.3:5424798-5424817 MS.gene072695:CDS 50.0%
TGAGCGAGGAGGATGAGTGT+AGG + chr7.3:5425211-5425230 MS.gene072695:CDS 55.0%
! GCAAGCACTTGCTGAGAGAC+AGG + chr7.3:5424789-5424808 MS.gene072695:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 5423848 5425518 5423848 ID=MS.gene072695
chr7.3 mRNA 5423848 5425518 5423848 ID=MS.gene072695.t1;Parent=MS.gene072695
chr7.3 exon 5423848 5425518 5423848 ID=MS.gene072695.t1.exon1;Parent=MS.gene072695.t1
chr7.3 CDS 5423848 5425518 5423848 ID=cds.MS.gene072695.t1;Parent=MS.gene072695.t1
Gene Sequence

>MS.gene072695

ATGGAAATTGACCATTTTTCTTCCACTCATGACTTTGTAGAAGAAGTTGTGTTCCAAGACAAACAACATAGTTTTTCTGGAATACAATTGTTGGAAGAAATTAACAATCCTCTTCCAAATGAGAATGAACAACTACCCAACATGTTCAACAACCAAAATCATGATCAACAACAACAAGAATTCGAAGTTGGTAATTTCTTGTCATTAGTAGAAGATATCAACCTAGAAACTAATCAACAAAATAATCATCACCACAAAGTTCCAATTCCAAAACCTTTAGAAAACAATATTCAGAAGATGCCACAATATTTATCATCCTTAGAGATTCTGAGAAATCATGGAAGCAGATTCAACAAGAAAAACACAATCAGTACTAAAGCTTCTTCAAATTTGGAACAGCATCAAAAGATGTCAACAGAAGGAATAATAAGAGTAGCTGGAGCAAGGTATACTCAATATTCTTCTTCTCATTGGAGTGAAAATTTTTGCATCCAAACACACCCTTATAGATTTGACCTTAATGGTTTATCTGAGGAAGAAAATAGAGACATTGAACTTGCACAGTTTCTCTATGTAGCAGCTGAAAGAGTAAGTTTGCAACAATTTGAACGCGCAAAAAAATTACTTTTATATTGTCAATGGAATTCTTCCATCACAGGAAATTGTGTTCAAAGAATTGTCTTTCATTTTGCACAAGCACTTCAAGAGAGAATAGAGAAAGAAACAGGAAGAGTGATTAAAGGGTCAGATAAAAATGAAGAAAGTGAGTTACTTGACAAGATGGGTTCAAAAAAAGTACTTTTGTGTCATCAAAAACTTCCTTTTAATCAAGTTATGCAATTTACTGGAATACAAGCTATAGTTGAACATGTTAAGTTTGAGACTAAAATTCATCTAATAGATTTTGATATAAGAAGTGGGGTCCAGTGCATAGCTTTGATGCAAGCACTTGCTGAGAGACAGGATTGCATGGTTGAAATTTTTAAGGTAAGTGCTATTGGACTTAATACATGCAAGAACAAAATTGAAGAAACTGGCAAAAATTTAGCTAGTTTTGCTGAATCATTGAACTTGCCCTTTTTATATAAACCTGTTTTAGTAGAAGATATGTTGGAAATCAAAGAAGATGATTTTGAAATTGAAAAGGATGAAGCTGTTGCCGTTTATTCTCCATATTTTCTAAGGACTTTAATATCAAGGCAGAATTCTATGGAAAATTTGATGAGAGTTTTGAGATATATCAAACCATGTATAATGATAGTACTTGAAACTGAAGCAAGTCTTAATTCACAGTCTTTTGTGAACCGATTCGTTGAAGCGTTGTTCTTCTACTCGGCTTTTTTCGATATGGCTGAAACATGTATGAGCGAGGAGGATGAGTGTAGGATGATAACAGAAGGAATATTATCTGCAGGGATAAGAAATATTGTCGCGTCAGAAGGTAGAGAAAGGACTGTTAGAAATGTGAAAATTGATGTTTGGAGAAGATTCTTTGCAAGGTATAGAATGGTGGAAACTAGGTTTAGTGAGACATGTGTTTACCAAGCTGAATTGGTGTCTAAAGAGTTTGATCATGGGAAATTTTGCGATGTGGAAAAGAATGGGAAGTGTTTGATATTGGGATGGAAGGGGACTCCAATGTATTCAATCTCAGCTTGGAGGTTTCTTTGA
Protein sequence

>MS.gene072695.t1

MEIDHFSSTHDFVEEVVFQDKQHSFSGIQLLEEINNPLPNENEQLPNMFNNQNHDQQQQEFEVGNFLSLVEDINLETNQQNNHHHKVPIPKPLENNIQKMPQYLSSLEILRNHGSRFNKKNTISTKASSNLEQHQKMSTEGIIRVAGARYTQYSSSHWSENFCIQTHPYRFDLNGLSEEENRDIELAQFLYVAAERVSLQQFERAKKLLLYCQWNSSITGNCVQRIVFHFAQALQERIEKETGRVIKGSDKNEESELLDKMGSKKVLLCHQKLPFNQVMQFTGIQAIVEHVKFETKIHLIDFDIRSGVQCIALMQALAERQDCMVEIFKVSAIGLNTCKNKIEETGKNLASFAESLNLPFLYKPVLVEDMLEIKEDDFEIEKDEAVAVYSPYFLRTLISRQNSMENLMRVLRYIKPCIMIVLETEASLNSQSFVNRFVEALFFYSAFFDMAETCMSEEDECRMITEGILSAGIRNIVASEGRERTVRNVKIDVWRRFFARYRMVETRFSETCVYQAELVSKEFDHGKFCDVEKNGKCLILGWKGTPMYSISAWRFL