AlfalfaGEDB | MS.gene072775

M. sativa cultivar XinJiangDaYe / MS.gene072775

KEGG: -
GO: GO:0003674 GO:0005488 GO:0005515
PFAM: PF06203.17

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072775.t1	XP_003611074.1	90.1	252	23	2	1	251	1	251	5.60E-121	443.7

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
No data available in table

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072775.t1	G7KC16	90.1	252	23	2	1	251	1	251	4.0e-121	443.7

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene072775.t1	TR	Others

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072775.t1	MTR_5g010120	90.079	252	23	2	1	251	1	251	2.90e-158	439
MS.gene072775.t1	MTR_8g098725	49.505	202	69	5	65	240	97	291	1.27e-53	176
MS.gene072775.t1	MTR_8g098725	47.573	206	71	5	65	240	97	295	8.60e-52	171
MS.gene072775.t1	MTR_2g068730	54.545	88	37	1	148	232	136	223	4.04e-22	92.4
MS.gene072775.t1	MTR_2g096080	42.609	115	44	2	129	221	90	204	9.35e-20	85.9
MS.gene072775.t1	MTR_1g044785	47.917	96	41	2	141	236	107	193	1.84e-19	84.3
MS.gene072775.t1	MTR_1g073350	51.948	77	37	0	139	215	283	359	1.23e-18	84.7
MS.gene072775.t1	MTR_4g061823	37.584	149	65	4	89	221	58	194	3.31e-17	78.6
MS.gene072775.t1	MTR_4g061910	37.584	149	65	4	89	221	58	194	3.31e-17	78.6
MS.gene072775.t1	MTR_1g008220	47.126	87	39	2	138	220	194	277	2.87e-16	76.6

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene072775.t1	AT5G41380	48.370	184	73	6	58	221	83	264	5.95e-40	140
MS.gene072775.t1	AT1G63820	49.342	152	54	5	117	248	117	265	5.01e-35	127
MS.gene072775.t1	AT3G12890	53.191	94	41	2	154	247	121	211	5.14e-21	89.4
MS.gene072775.t1	AT1G04500	40.602	133	58	3	103	234	234	346	1.79e-19	87.0
MS.gene072775.t1	AT1G04500	40.602	133	58	3	103	234	234	346	1.79e-19	87.0
MS.gene072775.t1	AT1G04500	40.602	133	58	3	103	234	225	337	1.87e-19	86.7
MS.gene072775.t1	AT2G33350	66.667	57	19	0	163	219	301	357	9.98e-18	82.0
MS.gene072775.t1	AT2G33350	66.667	57	19	0	163	219	302	358	1.10e-17	82.0
MS.gene072775.t1	AT2G33350	66.667	57	19	0	163	219	290	346	1.15e-17	82.0
MS.gene072775.t1	AT2G33350	66.667	57	19	0	163	219	310	366	1.20e-17	82.0

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Find 54 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGAGGAATGTCAAAT+GGG	0.562447	5.3:+3844379	MS.gene072775:CDS
AAATTTCCAAGCTCACTGGT+TGG	0.575257	5.3:-3844331	None:intergenic
AATTCATGCCATTGGAAATT+TGG	0.108434	5.3:-3844514	None:intergenic
ACGTATGCGTGTTCGATTGT+CGG	0.459741	5.3:-3845238	None:intergenic
ACTACCAAAAGTTAATGCAG+AGG	0.669632	5.3:+3844416	MS.gene072775:CDS
ACTGTTTCAATGTAATTGAA+AGG	0.489675	5.3:-3844247	None:intergenic
ACTTCATCCGTTTGAGACCC+AGG	0.506527	5.3:-3844838	None:intergenic
AGAGAAATTTCCAAGCTCAC+TGG	0.521246	5.3:-3844335	None:intergenic
AGCTCCTCTGCATTAACTTT+TGG	0.308354	5.3:-3844420	None:intergenic
AGCTTACCATAACACACAGA+TGG	0.590460	5.3:+3844486	MS.gene072775:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACTGAGGAATGTCAAAT+GGG	+	chr5.3:3844379-3844398	MS.gene072775:CDS	30.0%
AATTCATGCCATTGGAAATT+TGG	-	chr5.3:3844517-3844536	None:intergenic	30.0%
AGAAAAAGAAAAGAAGGAAG+AGG	-	chr5.3:3844308-3844327	None:intergenic	30.0%
ATAAAAGAAAGTTGTTCCCT+CGG	+	chr5.3:3845057-3845076	MS.gene072775:intron	30.0%
ATTCATGCCATTGGAAATTT+GGG	-	chr5.3:3844516-3844535	None:intergenic	30.0%
GAAATTTCTCTACTTTGGAT+GGG	+	chr5.3:3844347-3844366	MS.gene072775:CDS	30.0%
GTTTAATACTTGCATCAATG+TGG	+	chr5.3:3845422-3845441	MS.gene072775:intron	30.0%
TGGAGAAGAAAAAGAAAAGA+AGG	-	chr5.3:3844314-3844333	None:intergenic	30.0%
TTACATTGAAACAGTTCAAG+AGG	+	chr5.3:3844255-3844274	MS.gene072775:CDS	30.0%
ACTACCAAAAGTTAATGCAG+AGG	+	chr5.3:3844416-3844435	MS.gene072775:CDS	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr5.3	gene	3844160	3845514	3844160	ID=MS.gene072775
chr5.3	mRNA	3844160	3845514	3844160	ID=MS.gene072775.t1;Parent=MS.gene072775
chr5.3	exon	3844160	3844603	3844160	ID=MS.gene072775.t1.exon1;Parent=MS.gene072775.t1
chr5.3	CDS	3844160	3844603	3844160	ID=cds.MS.gene072775.t1;Parent=MS.gene072775.t1
chr5.3	exon	3844826	3844960	3844826	ID=MS.gene072775.t1.exon2;Parent=MS.gene072775.t1
chr5.3	CDS	3844826	3844960	3844826	ID=cds.MS.gene072775.t1;Parent=MS.gene072775.t1
chr5.3	exon	3845216	3845350	3845216	ID=MS.gene072775.t1.exon3;Parent=MS.gene072775.t1
chr5.3	CDS	3845216	3845350	3845216	ID=cds.MS.gene072775.t1;Parent=MS.gene072775.t1
chr5.3	exon	3845473	3845514	3845473	ID=MS.gene072775.t1.exon4;Parent=MS.gene072775.t1
chr5.3	CDS	3845473	3845514	3845473	ID=cds.MS.gene072775.t1;Parent=MS.gene072775.t1

Gene Sequence

>MS.gene072775

ATGTCTTCTGATAGTGATATCTATAATATTGATATTTCTTTCCATACACATTCAAGCCCAAACAAAGTCTCTTATGATCCTTATTACCCTTTCAATTACATTGAAACAGTTCAAGAGGAATTGAACAATCCTCTTGACTCATTTTCCTCTTCCTTCTTTTCTTTTTCTTCTCCAACCAGTGAGCTTGGAAATTTCTCTACTTTGGATGGGTCTCGTTTGAAAACTGAGGAATGTCAAATGGGTGTAGATTACTCCTACTACCAAAAGTTAATGCAGAGGAGCTATAACTGTAACAATTTTCATGAGAAACCTGATTTTCCTTCTGAAGCTTACCATAACACACAGATGGACTCCCCAAATTTCCAATGGCATGAATTGAGCTCCCCCGAAAATAGTTTCTTCACCGGACAAATAAGAAGGGCGTGCAGTGCAGGAGATTTGCAGGTTAAAATAACCTAACACCGTGGTTTACCATATATTTTTTTGAGCATCCACACTTCAGATTAAAAAATGTATATGGTGTCCGATACGTATTAATTTAATCATTTTCATTTTTTAAATGTAGGGTTTGATCTCCGTGTTTATGTCTATTATTTGATAGTAGATGGTTTATTTATTTATTTATTTAATTTTATCTGTATTAAAGGTATGCTGTTTATTGATCAGAACATGGCAACACCTGGGTCTCAAACGGATGAAGTAAATTTCAAAGTAGGGCGTTACAGTCTGGAAGAAAGAAAAGAAAAAATCTCTAGATATAGAGCCAAGAGAAGCCGGAGGAAGTTCAACAAGATTATTAAGGTATAACACTAATTAACTACTGCGAGTTTGCGTATGCTCCAACATTACCTGAAAGGAGCATATCTGAACTCATTAACCACTGTTATAATGTTATATATAAAAGAAAGTTGTTCCCTCGGAAGAAAAAAAAATATCAACTTTACTAGTAATAATTGAAGGACGAGAATGATGTATTAGTTTATTAAAGATTCCACATATTTTTTGTTTCTAAATATGTGGTTTCGTTTGTTGTTTTTAATTGTGGTACCACAACAGTATGAATGCCGAAAGACACTAGCCGACAATCGAACACGCATACGTGGCAGATTCGCAAGTAACGACAAAACCAACGAGATTCCAAAACCTGTGTGTTCAAAAACAGAAAAATATCATCATGAAGACGAGTTCTGGGTAAGTACGATTTTATGTATTGAATCTTAACATTTACATTATTATGTAATTAATATTAACTAAATTAAACTGTTTAATACTTGCATCAATGTGGTGTTAATATTATTGAGGTTTTTATTTAGGTTGATTTCATTGAAGGGTTAAATGACGAGCACCTCTATTAA

Protein sequence

>MS.gene072775.t1

MSSDSDIYNIDISFHTHSSPNKVSYDPYYPFNYIETVQEELNNPLDSFSSSFFSFSSPTSELGNFSTLDGSRLKTEECQMGVDYSYYQKLMQRSYNCNNFHEKPDFPSEAYHNTQMDSPNFQWHELSSPENSFFTGQIRRACSAGDLQNMATPGSQTDEVNFKVGRYSLEERKEKISRYRAKRSRRKFNKIIKYECRKTLADNRTRIRGRFASNDKTNEIPKPVCSKTEKYHHEDEFWVDFIEGLNDEHLY