AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07286

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07286.t1 MTR_1g087040 94.737 152 6 1 1 152 1 150 5.11e-103 291
MS.gene07286.t1 MTR_7g109510 48.344 151 63 2 1 151 1 136 1.43e-31 110
MS.gene07286.t1 MTR_4g070860 36.090 133 70 2 15 132 16 148 9.86e-24 90.9
MS.gene07286.t1 MTR_1g022495 36.709 158 87 4 7 151 2 159 1.77e-23 90.1
MS.gene07286.t1 MTR_3g117120 44.211 95 53 0 15 109 16 110 5.45e-23 89.0
MS.gene07286.t1 MTR_8g086020 37.415 147 82 4 1 140 1 144 3.97e-22 86.7
MS.gene07286.t1 MTR_3g112220 41.772 79 46 0 26 104 1 79 1.93e-15 67.8
MS.gene07286.t1 MTR_7g029400 38.298 94 55 1 7 97 59 152 4.05e-14 66.6
MS.gene07286.t1 MTR_5g015090 36.667 90 53 1 8 93 36 125 2.35e-13 63.9
MS.gene07286.t1 MTR_8g015250 40.217 92 51 2 10 97 51 142 1.31e-12 62.4
MS.gene07286.t1 MTR_4g097440 32.292 96 62 1 26 121 72 164 5.47e-12 60.8
MS.gene07286.t1 MTR_7g017880 36.697 109 62 2 26 133 84 186 8.01e-12 60.5
MS.gene07286.t1 MTR_3g103360 34.375 96 63 0 13 108 40 135 8.27e-12 59.7
MS.gene07286.t1 MTR_1g017570 40.000 85 47 1 28 108 59 143 9.59e-11 56.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07286.t1 AT3G62420 45.614 114 59 2 27 140 23 133 2.54e-27 99.8
MS.gene07286.t1 AT2G18160 44.554 101 55 1 24 124 26 125 3.26e-24 92.4
MS.gene07286.t1 AT1G75390 40.000 120 68 1 26 141 38 157 2.93e-23 90.1
MS.gene07286.t1 AT4G34590 48.810 84 43 0 26 109 24 107 3.97e-22 87.0
MS.gene07286.t1 AT1G75390 50.000 88 39 2 10 93 19 105 1.20e-19 79.3
MS.gene07286.t1 AT5G38800 38.462 91 56 0 8 98 51 141 4.27e-13 63.5
MS.gene07286.t1 AT3G30530 37.755 98 55 1 7 98 53 150 7.28e-13 63.2
MS.gene07286.t1 AT2G22850 43.038 79 44 1 24 101 123 201 1.78e-11 60.1
MS.gene07286.t1 AT2G22850 43.038 79 44 1 24 101 123 201 1.78e-11 60.1
MS.gene07286.t1 AT5G15830 43.284 67 37 1 3 69 49 114 6.56e-11 58.2
MS.gene07286.t1 AT1G13600 36.364 110 65 2 10 119 67 171 8.08e-11 58.2

Find 32 sgRNAs with CRISPR-Local

Find 33 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGTGATGCCATAGAATAATT+TGG 0.207768 1.4:+69387933 None:intergenic
ACCTTATCCAAATTATTCTA+TGG 0.288233 1.4:-69387940 MS.gene07286:CDS
TCAGGTTCAGGTTCAGGTTC+AGG 0.293663 1.4:-69388323 MS.gene07286:CDS
GTAAGTTCAGGTTCAGGTTC+AGG 0.297252 1.4:-69388329 MS.gene07286:CDS
CTGTTGTTGCTTCCTCAGTC+TGG 0.324562 1.4:+69388220 None:intergenic
GAACCTGAACCTGAACTTAC+AGG 0.327424 1.4:+69388332 None:intergenic
CCGTCTCGCGCGGAGTATTA+AGG 0.331337 1.4:-69388150 MS.gene07286:CDS
ATGATCCTCTTCTCAAACCT+TGG 0.395871 1.4:-69387968 MS.gene07286:CDS
CAGAGCGCAGACTAAAGAAT+TGG 0.417439 1.4:-69388081 MS.gene07286:CDS
TTTCAATCTACCGGCTTCTC+CGG 0.421529 1.4:+69388184 None:intergenic
ATTCAACGTCCTGTAAGTTC+AGG 0.426014 1.4:-69388341 MS.gene07286:CDS
CGTCCTGTAAGTTCAGGTTC+AGG 0.436352 1.4:-69388335 MS.gene07286:CDS
AAGAATTGGAGGCTCAGTTT+CGG 0.469404 1.4:-69388067 MS.gene07286:CDS
GCCATAGAATAATTTGGATA+AGG 0.470081 1.4:+69387939 None:intergenic
TTCAGGAGGTTCAGACGGTA+TGG 0.508548 1.4:-69388306 MS.gene07286:CDS
GGAGGAAGCGTATGTGAAGA+TGG 0.510835 1.4:-69388123 MS.gene07286:CDS
TGGATCGTCTCGCGGATTCA+CGG 0.514018 1.4:+69388240 None:intergenic
CAGACTGAGGAAGCAACAAC+AGG 0.520449 1.4:-69388219 MS.gene07286:CDS
AGCGCAGACTAAAGAATTGG+AGG 0.533603 1.4:-69388078 MS.gene07286:CDS
GAAGATCTGACCGGAGAAGC+CGG 0.539519 1.4:-69388194 MS.gene07286:CDS
CGCGCGGAGTATTAAGGCGA+CGG 0.554524 1.4:-69388144 MS.gene07286:CDS
GGTTCAGGTTCAGGTTCAGG+AGG 0.572738 1.4:-69388320 MS.gene07286:CDS
CCTTAATACTCCGCGCGAGA+CGG 0.575439 1.4:+69388150 None:intergenic
AGAAGAGGATCATCAATCAA+AGG 0.595685 1.4:+69387978 None:intergenic
ATGAACCAAGGTTTGAGAAG+AGG 0.601064 1.4:+69387963 None:intergenic
TTTGGATAAGGTATGAACCA+AGG 0.606535 1.4:+69387951 None:intergenic
TCAGGTTCAGGAGGTTCAGA+CGG 0.613881 1.4:-69388311 MS.gene07286:CDS
CCTCAGTCTGGATCGTCTCG+CGG 0.620361 1.4:+69388232 None:intergenic
CCGCGAGACGATCCAGACTG+AGG 0.625229 1.4:-69388232 MS.gene07286:CDS
CAACAGGTTGAAGATCTGAC+CGG 0.629630 1.4:-69388203 MS.gene07286:CDS
TCGAGAATGACCGTCTCGCG+CGG 0.638159 1.4:-69388160 MS.gene07286:CDS
GCGGAGTATTAAGGCGACGG+AGG 0.691194 1.4:-69388141 MS.gene07286:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! ACCTTATCCAAATTATTCTA+TGG - chr1.4:69388324-69388343 MS.gene07286:CDS 25.0%
GCCATAGAATAATTTGGATA+AGG + chr1.4:69388328-69388347 None:intergenic 30.0%
TGTGATGCCATAGAATAATT+TGG + chr1.4:69388334-69388353 None:intergenic 30.0%
! ATTCTCGATTTTCAATCTAC+CGG + chr1.4:69388092-69388111 None:intergenic 30.0%
AGAAGAGGATCATCAATCAA+AGG + chr1.4:69388289-69388308 None:intergenic 35.0%
TTTGGATAAGGTATGAACCA+AGG + chr1.4:69388316-69388335 None:intergenic 35.0%
AAGAATTGGAGGCTCAGTTT+CGG - chr1.4:69388197-69388216 MS.gene07286:CDS 40.0%
ATGAACCAAGGTTTGAGAAG+AGG + chr1.4:69388304-69388323 None:intergenic 40.0%
ATGATCCTCTTCTCAAACCT+TGG - chr1.4:69388296-69388315 MS.gene07286:CDS 40.0%
ATTCAACGTCCTGTAAGTTC+AGG - chr1.4:69387923-69387942 MS.gene07286:CDS 40.0%
AGCGCAGACTAAAGAATTGG+AGG - chr1.4:69388186-69388205 MS.gene07286:CDS 45.0%
CAGAGCGCAGACTAAAGAAT+TGG - chr1.4:69388183-69388202 MS.gene07286:CDS 45.0%
GAACCTGAACCTGAACTTAC+AGG + chr1.4:69387935-69387954 None:intergenic 45.0%
! CAACAGGTTGAAGATCTGAC+CGG - chr1.4:69388061-69388080 MS.gene07286:CDS 45.0%
! GTAAGTTCAGGTTCAGGTTC+AGG - chr1.4:69387935-69387954 MS.gene07286:CDS 45.0%
!! TTTCAATCTACCGGCTTCTC+CGG + chr1.4:69388083-69388102 None:intergenic 45.0%
CAGACTGAGGAAGCAACAAC+AGG - chr1.4:69388045-69388064 MS.gene07286:CDS 50.0%
TCAGGTTCAGGAGGTTCAGA+CGG - chr1.4:69387953-69387972 MS.gene07286:CDS 50.0%
TTCAGGAGGTTCAGACGGTA+TGG - chr1.4:69387958-69387977 MS.gene07286:CDS 50.0%
! CGTCCTGTAAGTTCAGGTTC+AGG - chr1.4:69387929-69387948 MS.gene07286:CDS 50.0%
! CTGTTGTTGCTTCCTCAGTC+TGG + chr1.4:69388047-69388066 None:intergenic 50.0%
! GGAGGAAGCGTATGTGAAGA+TGG - chr1.4:69388141-69388160 MS.gene07286:CDS 50.0%
! TCAGGTTCAGGTTCAGGTTC+AGG - chr1.4:69387941-69387960 MS.gene07286:CDS 50.0%
CCTTAATACTCCGCGCGAGA+CGG + chr1.4:69388117-69388136 None:intergenic 55.0%
GAAGATCTGACCGGAGAAGC+CGG - chr1.4:69388070-69388089 MS.gene07286:CDS 55.0%
TGGATCGTCTCGCGGATTCA+CGG + chr1.4:69388027-69388046 None:intergenic 55.0%
! GGTTCAGGTTCAGGTTCAGG+AGG - chr1.4:69387944-69387963 MS.gene07286:CDS 55.0%
CCTCAGTCTGGATCGTCTCG+CGG + chr1.4:69388035-69388054 None:intergenic 60.0%
TCGAGAATGACCGTCTCGCG+CGG - chr1.4:69388104-69388123 MS.gene07286:CDS 60.0%
! CCGTCTCGCGCGGAGTATTA+AGG - chr1.4:69388114-69388133 MS.gene07286:CDS 60.0%
! CGCGCGGAGTATTAAGGCGA+CGG - chr1.4:69388120-69388139 MS.gene07286:CDS 60.0%
! GCGGAGTATTAAGGCGACGG+AGG - chr1.4:69388123-69388142 MS.gene07286:CDS 60.0%
CCGCGAGACGATCCAGACTG+AGG - chr1.4:69388032-69388051 MS.gene07286:CDS 65.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 69387914 69388372 69387914 ID=MS.gene07286
chr1.4 mRNA 69387914 69388372 69387914 ID=MS.gene07286.t1;Parent=MS.gene07286
chr1.4 exon 69387914 69388372 69387914 ID=MS.gene07286.t1.exon1;Parent=MS.gene07286.t1
chr1.4 CDS 69387914 69388372 69387914 ID=cds.MS.gene07286.t1;Parent=MS.gene07286.t1
Gene Sequence

>MS.gene07286

ATGGCTTCGATTCAACGTCCTGTAAGTTCAGGTTCAGGTTCAGGTTCAGGAGGTTCAGACGGTATGGATCTACAAATCGACGAACGAAAACGCAAGAGAATGCTGTCAAACCGTGAATCCGCGAGACGATCCAGACTGAGGAAGCAACAACAGGTTGAAGATCTGACCGGAGAAGCCGGTAGATTGAAAATCGAGAATGACCGTCTCGCGCGGAGTATTAAGGCGACGGAGGAAGCGTATGTGAAGATGGAAGCTGCTAATGATGTTATCAGAGCGCAGACTAAAGAATTGGAGGCTCAGTTTCGGTTTCTGAATTCTGTAATTGATGCTGCTGCTGCTGAAGAGACTAATAGTTTCTCTGTTGATGATGTGCCTTTGATTGATGATCCTCTTCTCAAACCTTGGTTCATACCTTATCCAAATTATTCTATGGCATCACATGAAATGATGCTGCGTTGA
Protein sequence

>MS.gene07286.t1

MASIQRPVSSGSGSGSGGSDGMDLQIDERKRKRMLSNRESARRSRLRKQQQVEDLTGEAGRLKIENDRLARSIKATEEAYVKMEAANDVIRAQTKELEAQFRFLNSVIDAAAAEETNSFSVDDVPLIDDPLLKPWFIPYPNYSMASHEMMLR