AlfalfaGEDB | MS.gene07295

M. sativa cultivar XinJiangDaYe / MS.gene07295

KEGG: -
GO: -
PFAM: PF03514.17

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07295.t1	RHN81027.1	97.5	480	12	0	8	487	41	520	8.50E-267	929.1

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07295.t1	Q8GXW1	27.1	398	258	8	99	485	169	545	5.4e-36	153.7

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07295.t1	G7ICX5	97.5	480	12	0	8	487	1	480	6.1e-267	929.1

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene07295.t1	TF	GRAS

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07295.t1	MTR_1g086970	97.500	480	12	0	8	487	1	480	0.0	976
MS.gene07295.t1	MTR_7g109580	58.761	468	172	9	29	487	101	556	0.0	525
MS.gene07295.t1	MTR_2g034250	45.272	497	242	10	15	487	97	587	1.57e-130	391
MS.gene07295.t1	MTR_2g034260	44.534	494	250	10	6	487	105	586	4.30e-130	390
MS.gene07295.t1	MTR_2g034280	44.490	490	241	9	20	487	97	577	5.74e-127	381
MS.gene07295.t1	MTR_4g104020	30.691	391	244	13	108	485	144	520	3.65e-41	154
MS.gene07295.t1	MTR_7g027190	28.967	397	261	11	100	485	287	673	1.77e-39	152
MS.gene07295.t1	MTR_3g065980	26.161	409	256	10	99	486	158	541	1.47e-38	147
MS.gene07295.t1	MTR_8g442410	29.136	405	263	12	92	485	134	525	2.02e-38	147
MS.gene07295.t1	MTR_7g062120	28.351	388	254	11	108	485	367	740	2.47e-34	137

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07295.t1	AT3G03450	27.068	399	259	8	99	486	169	546	3.55e-43	160
MS.gene07295.t1	AT1G66350	25.907	386	263	7	102	486	144	507	1.72e-42	158
MS.gene07295.t1	AT2G01570	25.954	393	266	8	99	486	210	582	3.39e-40	153
MS.gene07295.t1	AT5G17490	27.806	392	249	10	104	486	151	517	1.21e-39	150
MS.gene07295.t1	AT1G14920	26.768	396	259	9	99	486	158	530	3.22e-39	149
MS.gene07295.t1	AT3G46600	26.750	400	243	14	105	485	3	371	5.88e-35	134
MS.gene07295.t1	AT2G29065	26.703	367	239	9	136	484	273	627	6.97e-35	138
MS.gene07295.t1	AT5G59450	27.250	400	246	14	108	485	222	598	1.07e-34	137
MS.gene07295.t1	AT3G46600	26.799	403	245	14	102	485	176	547	1.32e-34	136
MS.gene07295.t1	AT3G46600	26.799	403	245	14	102	485	208	579	2.36e-34	136

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Find 68 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATGAGAGAAACAAGGAGA+CGG	0.612044	1.4:-69316667	MS.gene07295:CDS
AACAAACCTATTCGCAAACA+AGG	0.532041	1.4:+69316116	None:intergenic
AAGATGAAGAAAGGAAGGTT+AGG	0.502253	1.4:-69315969	MS.gene07295:CDS
AAGCCATGATATCAATTCCA+CGG	0.616214	1.4:-69317163	MS.gene07295:CDS
AAGCCGTGGAATTGATATCA+TGG	0.533713	1.4:+69317160	None:intergenic
AAGGCTAAGTTAGAAGAAAT+TGG	0.303167	1.4:-69316393	MS.gene07295:CDS
AATAATCTTTACATTCCTTA+TGG	0.249550	1.4:-69316945	MS.gene07295:CDS
AATCAAGTAATGCAATTTGC+TGG	0.354696	1.4:-69316579	MS.gene07295:CDS
AATGGTAAGTGTTTAATAAT+TGG	0.167586	1.4:-69315808	MS.gene07295:CDS
AATTGCATTACTTGATTGAA+AGG	0.446453	1.4:+69316585	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAGGCTAAGTTAGAAGAAAT+TGG	-	chr1.4:69316566-69316585	MS.gene07295:CDS	30.0%
AATCAAGTAATGCAATTTGC+TGG	-	chr1.4:69316380-69316399	MS.gene07295:CDS	30.0%
ACTATGGTATCAAATTCTGA+TGG	-	chr1.4:69316737-69316756	MS.gene07295:CDS	30.0%
ATGGAAGCAATATTATCTGA+AGG	-	chr1.4:69316944-69316963	MS.gene07295:CDS	30.0%
GAAGATATAATAACACTTGC+AGG	-	chr1.4:69315954-69315973	MS.gene07295:CDS	30.0%
GACGGAATTGATTGATAAAA+TGG	-	chr1.4:69316310-69316329	MS.gene07295:CDS	30.0%
GTGTTTAATAATTGGATGGA+AGG	-	chr1.4:69317159-69317178	MS.gene07295:CDS	30.0%
GTTACTAAAAAGTTTGACTG+TGG	-	chr1.4:69317106-69317125	MS.gene07295:CDS	30.0%
GTTTAATAATTGGATGGAAG+GGG	-	chr1.4:69317161-69317180	MS.gene07295:CDS	30.0%
TAGATCAAATGAGAGAAACA+AGG	-	chr1.4:69316286-69316305	MS.gene07295:CDS	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	69315759	69317222	69315759	ID=MS.gene07295
chr1.4	mRNA	69315759	69317222	69315759	ID=MS.gene07295.t1;Parent=MS.gene07295
chr1.4	exon	69315759	69317222	69315759	ID=MS.gene07295.t1.exon1;Parent=MS.gene07295.t1
chr1.4	CDS	69315759	69317222	69315759	ID=cds.MS.gene07295.t1;Parent=MS.gene07295.t1

Gene Sequence

>MS.gene07295

ATGGAGAAGTTACTACAAGATCTAGAGCAAGAAAATCAAGCCATGATATCAATTCCACGGCTTAAGCAGGTGCAAATGCCACCACCATCTTCTTTAGCATCACTTGAGCTATTGAAAAATTATGGAAGCAAGTTCAATAAGCAAAAAAGCAAAAGCAATGTTGAAACTTGTGTAAGTCCACAAAAGTTGTCAACTGAAGATATAATAACACTTGCAGGAACGAAGTATATCCAAAACTCATCACTGTGGCATGATAATAATCTTTACATTCCTTATGGAACTGTTCTTGGAAGCTTGTTGTCGGACGAAGAAAATAAACAAGTTGAACTAGCTCAGTTTGTTCTAGCAGCAGCTGAGAGAGTAGGTTGTCAACAATTTGAACGTGCAAACATGTTACTTTCACATTTTCATCTCATGAATGAAAGTGGAAATATTGGAAGCACAGTTCAGAGACTTCTTTTCCATTTCTCTCAAGCACTTCAAGAGAGAATCAACAGAGAAACCGGACGAGGAAAGATGAAACTGGATAGATCAAATGAGAGAAACAAGGAGACGGAATTGATTGATAAAATGGAATTAGATACTAACATAGTAGTTAAATGTCATCAAAAAATTCCTTTCAATCAAGTAATGCAATTTGCTGGTGTACAAGCTATAGTGGAACATGTTGCATCACAAACAAAAATCCATTTGATTCATCTTAATATTGGATGTGGAGTGATGAGTACATGTTTCATGCAAGCATTAGTAGACCGCAAGGAAAAGCCGGTTGAAATTCTGAAGATCACAGCTATTGGATTTTCTAACAAGGCTAAGTTAGAAGAAATTGGCAAAAGTTTAATGAGTTTTGCTGAATCCTTGAACATACCCTTTTTATATAACATAGTTTTTGTTGAAGATGCTATGGAGATTAAGTTAGATCAATTTGAAATTGAATATGATGAAGCTGTTGCTATTTATTCTCCATGTTCACTTAGGACTATGGTATCAAATTCTGATGGCTTGGAGAATTTGATGAAGGTGATAAGAAAAATGAAACCGTTTATTATGATAGTACTTGAACTTGAAGCAAACCATAATTCACCCTTGTTTGCGAATAGGTTTGTTGAAGCATTGTTCTTTTACTCAGCATTTTTTGACTGTGTTGATACTTGTATAAAAGAGGATTATGAGTGTAGAGTGATAATGGAAGCAATATTATCTGAAGGGATAAGAAACATTGTTGGTTTGGAAGATGAAGAAAGGAAGGTTAGGAATGTGAAGATTGATGTTTGGAGAAGGTTCTTTGCAAGGTATAGAATGGTAGAAACTGGATTCAGTGAGTCTTCAATTTATCAAGCTAATTTAGTTACTAAAAAGTTTGACTGTGGGAAGTTTTGTACTATAGACAAAAATGGTAAGTGTTTAATAATTGGATGGAAGGGGACTCCAATTCATTCAGTCTCAGCTTGGAAGTTTCTTTGA

Protein sequence

>MS.gene07295.t1

MEKLLQDLEQENQAMISIPRLKQVQMPPPSSLASLELLKNYGSKFNKQKSKSNVETCVSPQKLSTEDIITLAGTKYIQNSSLWHDNNLYIPYGTVLGSLLSDEENKQVELAQFVLAAAERVGCQQFERANMLLSHFHLMNESGNIGSTVQRLLFHFSQALQERINRETGRGKMKLDRSNERNKETELIDKMELDTNIVVKCHQKIPFNQVMQFAGVQAIVEHVASQTKIHLIHLNIGCGVMSTCFMQALVDRKEKPVEILKITAIGFSNKAKLEEIGKSLMSFAESLNIPFLYNIVFVEDAMEIKLDQFEIEYDEAVAIYSPCSLRTMVSNSDGLENLMKVIRKMKPFIMIVLELEANHNSPLFANRFVEALFFYSAFFDCVDTCIKEDYECRVIMEAILSEGIRNIVGLEDEERKVRNVKIDVWRRFFARYRMVETGFSESSIYQANLVTKKFDCGKFCTIDKNGKCLIIGWKGTPIHSVSAWKFL