AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073126

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073126.t1 MTR_2g060500 74.101 139 30 1 1 133 7 145 1.98e-71 216
MS.gene073126.t1 MTR_2g060540 73.381 139 31 1 1 133 7 145 4.74e-71 216
MS.gene073126.t1 MTR_2g084495 68.027 147 39 2 1 139 1 147 1.73e-66 207
MS.gene073126.t1 MTR_2g084525 62.143 140 47 1 1 134 1 140 1.90e-60 192
MS.gene073126.t1 MTR_2g084510 54.494 178 67 3 2 165 1 178 2.26e-60 192
MS.gene073126.t1 MTR_8g056180 61.224 147 47 3 133 279 202 338 1.37e-54 180
MS.gene073126.t1 MTR_1g052090 58.503 147 51 2 133 279 223 359 5.48e-45 155
MS.gene073126.t1 MTR_1g044605 62.609 115 37 2 133 247 277 385 1.35e-41 148
MS.gene073126.t1 MTR_7g102790 47.260 146 60 6 1 138 4 140 4.89e-34 123
MS.gene073126.t1 MTR_5g030130 44.966 149 59 6 5 140 36 174 9.16e-34 123
MS.gene073126.t1 MTR_1g062160 46.429 140 60 5 1 134 4 134 2.87e-33 120
MS.gene073126.t1 MTR_5g030130 44.444 135 59 4 5 133 36 160 6.82e-32 117
MS.gene073126.t1 MTR_4g078170 44.118 136 60 4 5 134 16 141 1.13e-31 117
MS.gene073126.t1 MTR_5g007230 42.647 136 62 4 5 134 22 147 6.81e-31 115
MS.gene073126.t1 MTR_8g066820 44.118 136 60 5 5 134 34 159 1.14e-29 112
MS.gene073126.t1 MTR_2g083710 62.903 62 23 0 2 63 4 65 2.03e-22 88.2
MS.gene073126.t1 MTR_2g083720 46.847 111 27 2 1 111 3 81 1.19e-20 84.3
MS.gene073126.t1 MTR_4g094680 38.971 136 72 5 6 135 24 154 4.37e-20 87.0
MS.gene073126.t1 MTR_2g055210 37.857 140 68 7 3 135 100 227 3.78e-18 83.2
MS.gene073126.t1 MTR_7g070630 39.669 121 64 3 143 262 307 419 2.57e-16 78.6
MS.gene073126.t1 MTR_4g116300 38.406 138 64 6 6 135 8 132 1.48e-15 74.7
MS.gene073126.t1 MTR_3g455890 41.414 99 55 2 165 262 316 412 4.07e-14 72.4
MS.gene073126.t1 MTR_7g105490 31.056 161 89 6 110 254 106 260 2.06e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073126.t1 AT1G43000 42.767 159 75 5 1 153 6 154 1.86e-36 129
MS.gene073126.t1 AT1G43000 42.767 159 75 5 1 153 13 161 1.98e-36 129
MS.gene073126.t1 AT4G17900 39.779 181 82 7 5 168 35 205 2.74e-32 119
MS.gene073126.t1 AT4G17900 39.779 181 82 7 5 168 21 191 4.54e-32 118
MS.gene073126.t1 AT1G21000 44.853 136 59 6 5 134 14 139 1.41e-29 112
MS.gene073126.t1 AT1G21000 44.853 136 59 6 5 134 17 142 1.64e-29 112
MS.gene073126.t1 AT1G32700 44.138 145 62 5 6 141 14 148 2.43e-29 110
MS.gene073126.t1 AT2G27930 43.165 139 67 4 6 135 6 141 5.41e-28 106
MS.gene073126.t1 AT2G27930 43.165 139 67 4 6 135 6 141 5.41e-28 106
MS.gene073126.t1 AT2G27930 43.066 137 66 4 6 133 6 139 5.75e-27 103
MS.gene073126.t1 AT2G27930 43.066 137 66 4 6 133 6 139 5.75e-27 103
MS.gene073126.t1 AT1G76590 41.176 136 64 6 5 134 19 144 3.82e-26 103
MS.gene073126.t1 AT1G32700 44.444 117 47 4 34 141 2 109 6.85e-21 87.8
MS.gene073126.t1 AT5G46710 31.579 190 91 8 6 167 30 208 1.03e-20 88.6
MS.gene073126.t1 AT5G46710 31.579 190 91 8 6 167 14 192 1.12e-20 87.8
MS.gene073126.t1 AT5G46710 35.556 135 70 5 6 133 30 154 1.81e-19 83.6
MS.gene073126.t1 AT1G31040 36.765 136 68 6 6 135 21 144 6.49e-17 78.6
MS.gene073126.t1 AT2G12646 36.029 136 70 6 6 135 8 132 2.30e-14 71.6
MS.gene073126.t1 AT2G12646 36.029 136 70 6 6 135 8 132 2.30e-14 71.6
MS.gene073126.t1 AT2G12646 36.029 136 70 6 6 135 5 129 2.75e-14 71.2
MS.gene073126.t1 AT3G60670 33.333 147 77 6 6 142 9 144 1.32e-13 69.3
MS.gene073126.t1 AT2G27930 45.455 77 37 2 62 135 70 144 1.28e-11 62.8
MS.gene073126.t1 AT2G27930 45.455 77 37 2 62 135 70 144 1.28e-11 62.8

Find 78 sgRNAs with CRISPR-Local

Find 196 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTTGGGAGGTATGGCTTGC+TGG 0.208894 2.2:+13187929 MS.gene073126:CDS
CCCGATGATGCTTGATTACT+TGG 0.265273 2.2:+13187912 MS.gene073126:CDS
TTACCAATCTTCATTCTCTT+AGG 0.283981 2.2:-13186003 None:intergenic
ACTCATTCTTCGCTTCGTTT+TGG 0.339624 2.2:-13185590 None:intergenic
TCATCGTCATCAGTGGCCTC+TGG 0.352918 2.2:-13188216 None:intergenic
AGGTATGGCTTGCTGGGCCC+AGG 0.391719 2.2:+13187936 MS.gene073126:CDS
AAGTGGAATCTACGAGATTT+CGG 0.396457 2.2:-13186065 None:intergenic
CTTGGGAGGTATGGCTTGCT+GGG 0.403773 2.2:+13187930 MS.gene073126:CDS
GCTTGATTACTTGGGAGGTA+TGG 0.408829 2.2:+13187921 MS.gene073126:CDS
CAACATGGAGGACTACATTA+TGG 0.417833 2.2:+13188080 MS.gene073126:CDS
ATGGATTGCCATTGCAATCA+AGG 0.427353 2.2:-13185626 None:intergenic
TACAAGCATCTTGACATCCT+TGG 0.432167 2.2:+13185916 MS.gene073126:CDS
CAATCTTCATTCTCTTAGGT+TGG 0.437011 2.2:-13185999 None:intergenic
GAAAGCTCCGCTCCAAAGTA+AGG 0.439419 2.2:-13187988 None:intergenic
CCTCCATGCTCTCTCTGAAC+CGG 0.440729 2.2:-13187835 None:intergenic
CAAATTGACCAACACTGACT+TGG 0.446173 2.2:-13185695 None:intergenic
TGTCAGCAACTTCTCGAGCC+AGG 0.456881 2.2:-13185535 None:intergenic
CATCGTCATCAGTGGCCTCT+GGG 0.467666 2.2:-13188215 None:intergenic
AGAGCATGGAGGACCATCTC+AGG 0.472145 2.2:+13187846 MS.gene073126:CDS
TGCATGGGCTGTGCCTGAGA+TGG 0.473291 2.2:-13187859 None:intergenic
TTGAGGCGCATCCTCCTGCA+TGG 0.474640 2.2:-13187875 None:intergenic
TATGTGGACGGTCCTTACTT+TGG 0.478000 2.2:+13187976 MS.gene073126:CDS
AGGATATGATGGTGGTGCCC+TGG 0.480086 2.2:+13185517 None:intergenic
ATTCAAGTTTGAGAAGAAGA+AGG 0.483391 2.2:+13187798 MS.gene073126:intron
CCCAAGTAATCAAGCATCAT+CGG 0.484975 2.2:-13187913 None:intergenic
CTTCGATCCGACTTTGCTCG+TGG 0.492312 2.2:+13188138 MS.gene073126:CDS
TATCAAAAGCATTGCTCTGA+AGG 0.497620 2.2:-13188041 None:intergenic
TTGTGATGAGTTTCCGTGCA+AGG 0.499988 2.2:-13185657 None:intergenic
CAACCTAAGAGAATGAAGAT+TGG 0.511036 2.2:+13186000 MS.gene073126:CDS
ATCTCAGGCACAGCCCATGC+AGG 0.517303 2.2:+13187861 MS.gene073126:CDS
TGCCAAAGAGCAAAAGTAAG+TGG 0.517393 2.2:-13186082 None:intergenic
ATCCGACTTTGCTCGTGGGA+AGG 0.521419 2.2:+13188143 MS.gene073126:CDS
CCAAGTAATCAAGCATCATC+GGG 0.523946 2.2:-13187912 None:intergenic
TGCTCTGAAGGGACCTCTGA+CGG 0.525412 2.2:-13188029 None:intergenic
AGCATCATCGGGTGTAGTGG+TGG 0.525480 2.2:-13187901 None:intergenic
GGGTGTAGTGGTGGGTATTG+AGG 0.525835 2.2:-13187892 None:intergenic
ATGGAACAGAGGAGACGTGC+TGG 0.526961 2.2:+13188099 MS.gene073126:CDS
TCCATTGTGTGGACCTTGCA+CGG 0.527943 2.2:+13185644 MS.gene073126:CDS
ACATCTGTGATGATGTATCC+TGG 0.531100 2.2:-13187954 None:intergenic
CAACACTGACTTGGATCACT+CGG 0.540238 2.2:-13185686 None:intergenic
GAGACGTGCTGGAGAGAGAG+AGG 0.542882 2.2:+13188110 MS.gene073126:CDS
TGCCTTCCCACGAGCAAAGT+CGG 0.543093 2.2:-13188145 None:intergenic
TTCGATCCGACTTTGCTCGT+GGG 0.549372 2.2:+13188139 MS.gene073126:CDS
CTGCTGATCAGCACCGTCAG+AGG 0.552662 2.2:+13188016 MS.gene073126:CDS
TTCAAGTTTGAGAAGAAGAA+GGG 0.557237 2.2:+13187799 MS.gene073126:intron
CATAATGTAGTCCTCCATGT+TGG 0.561721 2.2:-13188079 None:intergenic
CTGATGACGATGAGCAGTGA+TGG 0.566048 2.2:+13188226 MS.gene073126:CDS
CCACCGGTTCAGAGAGAGCA+TGG 0.567589 2.2:+13187832 MS.gene073126:CDS
GTCAGCAACTTCTCGAGCCA+GGG 0.568625 2.2:-13185534 None:intergenic
TGAGAAGAAGAAGGGTCATG+AGG 0.570909 2.2:+13187807 MS.gene073126:CDS
TCAAGCATCATCGGGTGTAG+TGG 0.579207 2.2:-13187904 None:intergenic
TCACATAAGTTTGAACTCCA+AGG 0.580665 2.2:-13185933 None:intergenic
GTTTGGCACTGAAGCCAACA+TGG 0.583564 2.2:+13188065 MS.gene073126:CDS
ATCAAAAGCATTGCTCTGAA+GGG 0.584370 2.2:-13188040 None:intergenic
CATCTGTGATGATGTATCCT+GGG 0.584898 2.2:-13187953 None:intergenic
CACGAGCAAAGTCGGATCGA+AGG 0.585532 2.2:-13188137 None:intergenic
CCATGCTCTCTCTGAACCGG+TGG 0.589368 2.2:-13187832 None:intergenic
GAGAAGAAGAAGGGTCATGA+GGG 0.593084 2.2:+13187808 MS.gene073126:CDS
GAAGGGTCATGAGGGACCAC+CGG 0.593346 2.2:+13187816 MS.gene073126:CDS
CCGATGATGCTTGATTACTT+GGG 0.599859 2.2:+13187913 MS.gene073126:CDS
CGAGTGATCCAAGTCAGTGT+TGG 0.601681 2.2:+13185687 MS.gene073126:CDS
TACATCATCACAGATGTATG+TGG 0.602379 2.2:+13187960 MS.gene073126:CDS
GCATCATCGGGTGTAGTGGT+GGG 0.603602 2.2:-13187900 None:intergenic
TGAGGCGCATCCTCCTGCAT+GGG 0.606036 2.2:-13187874 None:intergenic
ATGATGCTTGATTACTTGGG+AGG 0.611263 2.2:+13187916 MS.gene073126:CDS
TCACTGCTCATCGTCATCAG+TGG 0.616019 2.2:-13188223 None:intergenic
TCCGTGCAAGGTCCACACAA+TGG 0.632837 2.2:-13185645 None:intergenic
TGCAATGGCAATCCATTGTG+TGG 0.634353 2.2:+13185633 MS.gene073126:CDS
GGACGGTCCTTACTTTGGAG+CGG 0.634749 2.2:+13187981 MS.gene073126:CDS
TCAGGCACAGCCCATGCAGG+AGG 0.637198 2.2:+13187864 MS.gene073126:CDS
AGACGTGCTGGAGAGAGAGA+GGG 0.639721 2.2:+13188111 MS.gene073126:CDS
TGTTTCTTAGGATATGATGG+TGG 0.652828 2.2:+13185509 None:intergenic
CACTGACTTGGATCACTCGG+TGG 0.654344 2.2:-13185683 None:intergenic
TGGTTTGCAGGGTGACCCAG+AGG 0.666688 2.2:+13188200 MS.gene073126:CDS
AGGACTACATTATGGAACAG+AGG 0.684776 2.2:+13188088 MS.gene073126:CDS
TCATCACAGATGTATGTGGA+CGG 0.694083 2.2:+13187964 MS.gene073126:CDS
TGGCACTGAAGCCAACATGG+AGG 0.752121 2.2:+13188068 MS.gene073126:CDS
CCGGTTCAGAGAGAGCATGG+AGG 0.794370 2.2:+13187835 MS.gene073126:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATAAATAAATAAAAAAAT+TGG + chr2.2:13186371-13186390 MS.gene073126:intron 0.0%
!! AATAAATAAATAAAAAAATT+GGG + chr2.2:13186372-13186391 MS.gene073126:intron 0.0%
!! AAAAATAAAATGAATAGAAA+CGG - chr2.2:13185772-13185791 None:intergenic 10.0%
!!! TTTGATTATTTTTTTGAATT+TGG + chr2.2:13185808-13185827 MS.gene073126:intron 10.0%
!! AAAAAAAATCAAAAATCCAT+GGG - chr2.2:13187708-13187727 None:intergenic 15.0%
!! AAAATAAAATGAATAGAAAC+GGG - chr2.2:13185771-13185790 None:intergenic 15.0%
!! ACTATATGAATAAATTTCAA+GGG - chr2.2:13187355-13187374 None:intergenic 15.0%
!! GATATAAAAAATATAGCATA+TGG - chr2.2:13187313-13187332 None:intergenic 15.0%
!! TACTATATGAATAAATTTCA+AGG - chr2.2:13187356-13187375 None:intergenic 15.0%
!!! ACTTTTTTTGTTTTTGTTTT+TGG - chr2.2:13186581-13186600 None:intergenic 15.0%
!!! AGTAATATTATTTCTCTAAA+AGG + chr2.2:13187372-13187391 MS.gene073126:intron 15.0%
!!! TATTTGACTTTTGAAAATAA+TGG + chr2.2:13186681-13186700 MS.gene073126:intron 15.0%
!!! TGATATTTAAATGAGTATTT+TGG + chr2.2:13186341-13186360 MS.gene073126:intron 15.0%
!!! TGTTTGTTACAAAATAAAAA+AGG - chr2.2:13186614-13186633 None:intergenic 15.0%
!! CAAAAAAAATCAAAAATCCA+TGG - chr2.2:13187709-13187728 None:intergenic 20.0%
!! CTTGAATTATAAATCAGATA+AGG + chr2.2:13185854-13185873 MS.gene073126:intron 20.0%
!! TTTGCATATATAAAAGAGAA+TGG + chr2.2:13186809-13186828 MS.gene073126:intron 20.0%
!!! ACTTAATTTAAAACAGTGTT+AGG + chr2.2:13186288-13186307 MS.gene073126:intron 20.0%
!!! GTTCAATTTTTTTTTGTTGA+GGG - chr2.2:13187291-13187310 None:intergenic 20.0%
!!! TATGGATTATGAAATTTGAA+TGG - chr2.2:13187776-13187795 None:intergenic 20.0%
! CAAAATCAGTGATATGATAA+AGG - chr2.2:13186234-13186253 None:intergenic 25.0%
! CTCTTGAATATGAATAAGTA+CGG - chr2.2:13186530-13186549 None:intergenic 25.0%
! TCTAAAAGGTCAAATGTATT+TGG + chr2.2:13187386-13187405 MS.gene073126:intron 25.0%
! TGAATGGAATATGTTATGTA+TGG - chr2.2:13187760-13187779 None:intergenic 25.0%
!!! AACTTTTTAGCCTTTTCTTT+TGG - chr2.2:13186908-13186927 None:intergenic 25.0%
!!! GGTTCAATTTTTTTTTGTTG+AGG - chr2.2:13187292-13187311 None:intergenic 25.0%
ACATCATTATTCCTACCAAT+TGG - chr2.2:13186479-13186498 None:intergenic 30.0%
AGAAAATGAAGAGAATGTAG+AGG + chr2.2:13186976-13186995 MS.gene073126:intron 30.0%
ATATGAATAAGTACGGTGTA+TGG - chr2.2:13186523-13186542 None:intergenic 30.0%
CGTTCAAATTCAAGAAGAAA+AGG + chr2.2:13187070-13187089 MS.gene073126:intron 30.0%
CTTGAATTTGAACGATTAAC+AGG - chr2.2:13187064-13187083 None:intergenic 30.0%
GAAGTCACTTGATTGAAAAT+TGG - chr2.2:13186191-13186210 None:intergenic 30.0%
GCATATATAAAAGAGAATGG+AGG + chr2.2:13186812-13186831 MS.gene073126:intron 30.0%
GGAAGAAATGAAAAGATTAC+TGG + chr2.2:13186859-13186878 MS.gene073126:intron 30.0%
GGTATTAACACCAAAAGAAA+AGG + chr2.2:13186895-13186914 MS.gene073126:intron 30.0%
TATGAATAAGTACGGTGTAT+GGG - chr2.2:13186522-13186541 None:intergenic 30.0%
TTACCAATCTTCATTCTCTT+AGG - chr2.2:13186006-13186025 None:intergenic 30.0%
TTCTTCTTCTCAAACTTGAA+TGG - chr2.2:13187799-13187818 None:intergenic 30.0%
! ACTTTTTGCTTGAACATGAT+CGG + chr2.2:13187524-13187543 MS.gene073126:intron 30.0%
! ATTCAAGTTTGAGAAGAAGA+AGG + chr2.2:13187798-13187817 MS.gene073126:intron 30.0%
! GACAATCACAACATTTTTCA+TGG + chr2.2:13185554-13185573 MS.gene073126:CDS 30.0%
! TCCTGATCTCTAAAAAAATG+TGG + chr2.2:13187464-13187483 MS.gene073126:intron 30.0%
! TTCAAGTTTGAGAAGAAGAA+GGG + chr2.2:13187799-13187818 MS.gene073126:intron 30.0%
!!! ACCACATTTTTTTAGAGATC+AGG - chr2.2:13187468-13187487 None:intergenic 30.0%
AAGAAAAGGAATACCTCATC+GGG + chr2.2:13187084-13187103 MS.gene073126:intron 35.0%
AAGGGAAAAAAATGAGATCG+AGG - chr2.2:13186126-13186145 None:intergenic 35.0%
AAGTGGAATCTACGAGATTT+CGG - chr2.2:13186068-13186087 None:intergenic 35.0%
ATATCTGTAACCCTTTGACT+TGG - chr2.2:13187128-13187147 None:intergenic 35.0%
ATGAATAAGTACGGTGTATG+GGG - chr2.2:13186521-13186540 None:intergenic 35.0%
ATTACTGGAGAAATCGATCA+AGG + chr2.2:13186874-13186893 MS.gene073126:intron 35.0%
CAACCTAAGAGAATGAAGAT+TGG + chr2.2:13186000-13186019 MS.gene073126:CDS 35.0%
CAATCTTCATTCTCTTAGGT+TGG - chr2.2:13186002-13186021 None:intergenic 35.0%
CGGGTATCATATTTATCCAA+CGG - chr2.2:13187606-13187625 None:intergenic 35.0%
CTTCTCAAACTTGAATGGTA+TGG - chr2.2:13187794-13187813 None:intergenic 35.0%
GAAGAAAAGGAATACCTCAT+CGG + chr2.2:13187083-13187102 MS.gene073126:intron 35.0%
GGGTATCATATTTATCCAAC+GGG - chr2.2:13187605-13187624 None:intergenic 35.0%
GGTAGGAATAATGATGTGTA+GGG + chr2.2:13186482-13186501 MS.gene073126:intron 35.0%
GGTATCATATTTATCCAACG+GGG - chr2.2:13187604-13187623 None:intergenic 35.0%
GTAGGAATAATGATGTGTAG+GGG + chr2.2:13186483-13186502 MS.gene073126:intron 35.0%
TAAAAAAAAGTTGCCCGATG+AGG - chr2.2:13187100-13187119 None:intergenic 35.0%
TACATCATCACAGATGTATG+TGG + chr2.2:13187960-13187979 MS.gene073126:CDS 35.0%
TCACATAAGTTTGAACTCCA+AGG - chr2.2:13185936-13185955 None:intergenic 35.0%
TGGTAGGAATAATGATGTGT+AGG + chr2.2:13186481-13186500 MS.gene073126:intron 35.0%
TTTCTTGAACGAAGAAGAGA+TGG + chr2.2:13186838-13186857 MS.gene073126:intron 35.0%
! AAGTTTTGCCTTGATTGCAA+TGG + chr2.2:13185618-13185637 MS.gene073126:CDS 35.0%
! AGCAATGCTTTTGATAGGTT+TGG + chr2.2:13188048-13188067 MS.gene073126:CDS 35.0%
! AGGGTAAGAGCATAAATGTA+CGG - chr2.2:13187336-13187355 None:intergenic 35.0%
! ATCGGTTTAAGGATGTCAAT+GGG + chr2.2:13187542-13187561 MS.gene073126:intron 35.0%
! TATCTGTAACCCTTTGACTT+GGG - chr2.2:13187127-13187146 None:intergenic 35.0%
! TTCAGAGCAATGCTTTTGAT+AGG + chr2.2:13188043-13188062 MS.gene073126:CDS 35.0%
!! ATCAAAAGCATTGCTCTGAA+GGG - chr2.2:13188043-13188062 None:intergenic 35.0%
!! TATCAAAAGCATTGCTCTGA+AGG - chr2.2:13188044-13188063 None:intergenic 35.0%
!! TTCCACTTACTTTTGCTCTT+TGG + chr2.2:13186080-13186099 MS.gene073126:CDS 35.0%
!!! CATCGGGCAACTTTTTTTTA+AGG + chr2.2:13187100-13187119 MS.gene073126:intron 35.0%
!!! CGATCTCATTTTTTTCCCTT+GGG + chr2.2:13186126-13186145 MS.gene073126:intron 35.0%
!!! GATCTCATTTTTTTCCCTTG+GGG + chr2.2:13186127-13186146 MS.gene073126:intron 35.0%
!!! TCGATCTCATTTTTTTCCCT+TGG + chr2.2:13186125-13186144 MS.gene073126:intron 35.0%
!!! TTTTTTTGTTGAGGGAAGCA+TGG - chr2.2:13187283-13187302 None:intergenic 35.0%
AGGACTACATTATGGAACAG+AGG + chr2.2:13188088-13188107 MS.gene073126:CDS 40.0%
ATCATATTTATCCAACGGGG+CGG - chr2.2:13187601-13187620 None:intergenic 40.0%
ATCTCTATACGGGTAATCCA+CGG + chr2.2:13187651-13187670 MS.gene073126:intron 40.0%
ATGATGCTTGATTACTTGGG+AGG + chr2.2:13187916-13187935 MS.gene073126:CDS 40.0%
CAAAAATCCATGGGTATTCG+CGG - chr2.2:13187699-13187718 None:intergenic 40.0%
CAACATGGAGGACTACATTA+TGG + chr2.2:13188080-13188099 MS.gene073126:CDS 40.0%
CATAATGTAGTCCTCCATGT+TGG - chr2.2:13188082-13188101 None:intergenic 40.0%
CCAAGTAATCAAGCATCATC+GGG - chr2.2:13187915-13187934 None:intergenic 40.0%
CCCAAGTAATCAAGCATCAT+CGG - chr2.2:13187916-13187935 None:intergenic 40.0%
CCGATGATGCTTGATTACTT+GGG + chr2.2:13187913-13187932 MS.gene073126:CDS 40.0%
TACAAGCATCTTGACATCCT+TGG + chr2.2:13185916-13185935 MS.gene073126:CDS 40.0%
TCATCACAGATGTATGTGGA+CGG + chr2.2:13187964-13187983 MS.gene073126:CDS 40.0%
TGCCAAAGAGCAAAAGTAAG+TGG - chr2.2:13186085-13186104 None:intergenic 40.0%
! ACATCTGTGATGATGTATCC+TGG - chr2.2:13187957-13187976 None:intergenic 40.0%
! ACTCATTCTTCGCTTCGTTT+TGG - chr2.2:13185593-13185612 None:intergenic 40.0%
! ATGGATTGCCATTGCAATCA+AGG - chr2.2:13185629-13185648 None:intergenic 40.0%
! CAAATTGACCAACACTGACT+TGG - chr2.2:13185698-13185717 None:intergenic 40.0%
! CATCTGTGATGATGTATCCT+GGG - chr2.2:13187956-13187975 None:intergenic 40.0%
! CTTTTCAACCTTAGTGATCC+GGG - chr2.2:13186168-13186187 None:intergenic 40.0%
! GATCGGTTTAAGGATGTCAA+TGG + chr2.2:13187541-13187560 MS.gene073126:intron 40.0%
! GCTTGAACATGATCGGTTTA+AGG + chr2.2:13187531-13187550 MS.gene073126:intron 40.0%
! GCTTTTCAACCTTAGTGATC+CGG - chr2.2:13186169-13186188 None:intergenic 40.0%
! GGGAAGCATGGTTCAAATAT+TGG - chr2.2:13187271-13187290 None:intergenic 40.0%
! GGGTAAGAGCATAAATGTAC+GGG - chr2.2:13187335-13187354 None:intergenic 40.0%
! TATTCCTACCAATTGGCTCA+CGG - chr2.2:13186472-13186491 None:intergenic 40.0%
!! AGCGTGAATGAGTTTTATGC+TGG + chr2.2:13188180-13188199 MS.gene073126:CDS 40.0%
!! ATCTGTAACCCTTTGACTTG+GGG - chr2.2:13187126-13187145 None:intergenic 40.0%
!!! GAGTTTTATGCTGGTTTGCA+GGG + chr2.2:13188189-13188208 MS.gene073126:CDS 40.0%
!!! TGAGTTTTATGCTGGTTTGC+AGG + chr2.2:13188188-13188207 MS.gene073126:CDS 40.0%
!!! TTTTAAGGCCCCAAGTCAAA+GGG + chr2.2:13187115-13187134 MS.gene073126:intron 40.0%
!!! TTTTTAAGGCCCCAAGTCAA+AGG + chr2.2:13187114-13187133 MS.gene073126:intron 40.0%
CACGGGTATTTAGATACCCA+CGG + chr2.2:13187669-13187688 MS.gene073126:intron 45.0%
CCCGATGATGCTTGATTACT+TGG + chr2.2:13187912-13187931 MS.gene073126:CDS 45.0%
GAGAAGAAGAAGGGTCATGA+GGG + chr2.2:13187808-13187827 MS.gene073126:CDS 45.0%
GCTTGATTACTTGGGAGGTA+TGG + chr2.2:13187921-13187940 MS.gene073126:CDS 45.0%
GGAGGAGAAAGACAAGAAGA+CGG + chr2.2:13187019-13187038 MS.gene073126:intron 45.0%
TATGTGGACGGTCCTTACTT+TGG + chr2.2:13187976-13187995 MS.gene073126:CDS 45.0%
TCATATTTATCCAACGGGGC+GGG - chr2.2:13187600-13187619 None:intergenic 45.0%
TCTCTATACGGGTAATCCAC+GGG + chr2.2:13187652-13187671 MS.gene073126:intron 45.0%
TGAAGAGAATGTAGAGGACG+AGG + chr2.2:13186982-13187001 MS.gene073126:intron 45.0%
TGAGAAGAAGAAGGGTCATG+AGG + chr2.2:13187807-13187826 MS.gene073126:CDS 45.0%
TGCAATGGCAATCCATTGTG+TGG + chr2.2:13185633-13185652 MS.gene073126:CDS 45.0%
TTCGTGCGGGTATCTCTATA+CGG + chr2.2:13187640-13187659 MS.gene073126:intron 45.0%
TTGTGATGAGTTTCCGTGCA+AGG - chr2.2:13185660-13185679 None:intergenic 45.0%
! CATTCTTCGCTTCGTTTTGG+TGG - chr2.2:13185590-13185609 None:intergenic 45.0%
! CTTTTGCTCTTTGGCATGCA+AGG + chr2.2:13186089-13186108 MS.gene073126:CDS 45.0%
!! CAACACTGACTTGGATCACT+CGG - chr2.2:13185689-13185708 None:intergenic 45.0%
ACGAATATGAGGACGGGTAC+GGG - chr2.2:13187625-13187644 None:intergenic 50.0%
AGGATGTCAATGGGTATCCG+TGG + chr2.2:13187551-13187570 MS.gene073126:intron 50.0%
ATGTGTCTCCGTGAGCCAAT+TGG + chr2.2:13186461-13186480 MS.gene073126:intron 50.0%
CACGAATATGAGGACGGGTA+CGG - chr2.2:13187626-13187645 None:intergenic 50.0%
CATATTTATCCAACGGGGCG+GGG - chr2.2:13187599-13187618 None:intergenic 50.0%
CGAGTGATCCAAGTCAGTGT+TGG + chr2.2:13185687-13185706 MS.gene073126:CDS 50.0%
CGTGGGTATCTAAATACCCG+TGG - chr2.2:13187671-13187690 None:intergenic 50.0%
GAAAGCTCCGCTCCAAAGTA+AGG - chr2.2:13187991-13188010 None:intergenic 50.0%
GACGAGGATGATGAAGAAGG+AGG + chr2.2:13186998-13187017 MS.gene073126:intron 50.0%
GAGATACCCGCACGAATATG+AGG - chr2.2:13187636-13187655 None:intergenic 50.0%
GAGGACGAGGATGATGAAGA+AGG + chr2.2:13186995-13187014 MS.gene073126:intron 50.0%
GAGGATGATGAAGAAGGAGG+AGG + chr2.2:13187001-13187020 MS.gene073126:intron 50.0%
GGATGTCAATGGGTATCCGT+GGG + chr2.2:13187552-13187571 MS.gene073126:intron 50.0%
TACCCGCACGAATATGAGGA+CGG - chr2.2:13187632-13187651 None:intergenic 50.0%
TACCCGTCCTCATATTCGTG+CGG + chr2.2:13187626-13187645 MS.gene073126:intron 50.0%
TATCATACTGCCCGTACCCA+CGG - chr2.2:13187571-13187590 None:intergenic 50.0%
TCAAGCATCATCGGGTGTAG+TGG - chr2.2:13187907-13187926 None:intergenic 50.0%
TCAATGGGTATCCGTGGGTA+CGG + chr2.2:13187557-13187576 MS.gene073126:intron 50.0%
TCACTGCTCATCGTCATCAG+TGG - chr2.2:13188226-13188245 None:intergenic 50.0%
TCCATTGTGTGGACCTTGCA+CGG + chr2.2:13185644-13185663 MS.gene073126:CDS 50.0%
TCGAGACTATCACACCCCAA+GGG - chr2.2:13186144-13186163 None:intergenic 50.0%
TCGTGCGGGTATCTCTATAC+GGG + chr2.2:13187641-13187660 MS.gene073126:intron 50.0%
TTCGAGACTATCACACCCCA+AGG - chr2.2:13186145-13186164 None:intergenic 50.0%
TTCGATCCGACTTTGCTCGT+GGG + chr2.2:13188139-13188158 MS.gene073126:CDS 50.0%
! GTTTGGCACTGAAGCCAACA+TGG + chr2.2:13188065-13188084 MS.gene073126:CDS 50.0%
ACCCGCACGAATATGAGGAC+GGG - chr2.2:13187631-13187650 None:intergenic 55.0%
ACCCGTCCTCATATTCGTGC+GGG + chr2.2:13187627-13187646 MS.gene073126:intron 55.0%
AGACGTGCTGGAGAGAGAGA+GGG + chr2.2:13188111-13188130 MS.gene073126:CDS 55.0%
AGAGCATGGAGGACCATCTC+AGG + chr2.2:13187846-13187865 MS.gene073126:CDS 55.0%
AGCATCATCGGGTGTAGTGG+TGG - chr2.2:13187904-13187923 None:intergenic 55.0%
ATCCGACTTTGCTCGTGGGA+AGG + chr2.2:13188143-13188162 MS.gene073126:CDS 55.0%
ATGGAACAGAGGAGACGTGC+TGG + chr2.2:13188099-13188118 MS.gene073126:CDS 55.0%
CAATGGGTATCCGTGGGTAC+GGG + chr2.2:13187558-13187577 MS.gene073126:intron 55.0%
CACGAGCAAAGTCGGATCGA+AGG - chr2.2:13188140-13188159 None:intergenic 55.0%
CATCGTCATCAGTGGCCTCT+GGG - chr2.2:13188218-13188237 None:intergenic 55.0%
CCTCCATGCTCTCTCTGAAC+CGG - chr2.2:13187838-13187857 None:intergenic 55.0%
CGGATATCCGCGAATACCCA+TGG + chr2.2:13187689-13187708 MS.gene073126:intron 55.0%
CTTCGATCCGACTTTGCTCG+TGG + chr2.2:13188138-13188157 MS.gene073126:CDS 55.0%
GCATCATCGGGTGTAGTGGT+GGG - chr2.2:13187903-13187922 None:intergenic 55.0%
GGGTATTCGCGGATATCCGT+GGG - chr2.2:13187688-13187707 None:intergenic 55.0%
GGGTGTGATAGTCTCGAACC+CGG + chr2.2:13186147-13186166 MS.gene073126:intron 55.0%
GTCAGCAACTTCTCGAGCCA+GGG - chr2.2:13185537-13185556 None:intergenic 55.0%
GTCTCCGTGAGCCAATTGGT+AGG + chr2.2:13186465-13186484 MS.gene073126:intron 55.0%
GTCTCGAACCCGGATCACTA+AGG + chr2.2:13186157-13186176 MS.gene073126:intron 55.0%
TCATCGTCATCAGTGGCCTC+TGG - chr2.2:13188219-13188238 None:intergenic 55.0%
TCCGTGCAAGGTCCACACAA+TGG - chr2.2:13185648-13185667 None:intergenic 55.0%
TGCCTTCCCACGAGCAAAGT+CGG - chr2.2:13188148-13188167 None:intergenic 55.0%
TGGGTATTCGCGGATATCCG+TGG - chr2.2:13187689-13187708 None:intergenic 55.0%
TGTCAGCAACTTCTCGAGCC+AGG - chr2.2:13185538-13185557 None:intergenic 55.0%
! ACTTGGGAGGTATGGCTTGC+TGG + chr2.2:13187929-13187948 MS.gene073126:CDS 55.0%
! GGACGGTCCTTACTTTGGAG+CGG + chr2.2:13187981-13188000 MS.gene073126:CDS 55.0%
! TGGCACTGAAGCCAACATGG+AGG + chr2.2:13188068-13188087 MS.gene073126:CDS 55.0%
!! CACTGACTTGGATCACTCGG+TGG - chr2.2:13185686-13185705 None:intergenic 55.0%
!! CTTGGGAGGTATGGCTTGCT+GGG + chr2.2:13187930-13187949 MS.gene073126:CDS 55.0%
!! GGGTGTAGTGGTGGGTATTG+AGG - chr2.2:13187895-13187914 None:intergenic 55.0%
!! TGCTCTGAAGGGACCTCTGA+CGG - chr2.2:13188032-13188051 None:intergenic 55.0%
ATCTCAGGCACAGCCCATGC+AGG + chr2.2:13187861-13187880 MS.gene073126:CDS 60.0%
CCATGCTCTCTCTGAACCGG+TGG - chr2.2:13187835-13187854 None:intergenic 60.0%
CTGCTGATCAGCACCGTCAG+AGG + chr2.2:13188016-13188035 MS.gene073126:CDS 60.0%
GAAGGGTCATGAGGGACCAC+CGG + chr2.2:13187816-13187835 MS.gene073126:CDS 60.0%
GAGACGTGCTGGAGAGAGAG+AGG + chr2.2:13188110-13188129 MS.gene073126:CDS 60.0%
TGAGGCGCATCCTCCTGCAT+GGG - chr2.2:13187877-13187896 None:intergenic 60.0%
TTGAGGCGCATCCTCCTGCA+TGG - chr2.2:13187878-13187897 None:intergenic 60.0%
TTTATCCAACGGGGCGGGGA+CGG - chr2.2:13187595-13187614 None:intergenic 60.0%
! CCGGTTCAGAGAGAGCATGG+AGG + chr2.2:13187835-13187854 MS.gene073126:CDS 60.0%
! TGCATGGGCTGTGCCTGAGA+TGG - chr2.2:13187862-13187881 None:intergenic 60.0%
! TGGTTTGCAGGGTGACCCAG+AGG + chr2.2:13188200-13188219 MS.gene073126:CDS 60.0%
!! CCACCGGTTCAGAGAGAGCA+TGG + chr2.2:13187832-13187851 MS.gene073126:CDS 60.0%
TCAGGCACAGCCCATGCAGG+AGG + chr2.2:13187864-13187883 MS.gene073126:CDS 65.0%
!! AGGTATGGCTTGCTGGGCCC+AGG + chr2.2:13187936-13187955 MS.gene073126:CDS 65.0%
GATATCCGTCCCCGCCCCGT+TGG + chr2.2:13187587-13187606 MS.gene073126:intron 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 13185522 13188245 13185522 ID=MS.gene073126
chr2.2 mRNA 13185522 13188245 13185522 ID=MS.gene073126.t1;Parent=MS.gene073126
chr2.2 exon 13185522 13185708 13185522 ID=MS.gene073126.t1.exon1;Parent=MS.gene073126.t1
chr2.2 CDS 13185522 13185708 13185522 ID=cds.MS.gene073126.t1;Parent=MS.gene073126.t1
chr2.2 exon 13185899 13186110 13185899 ID=MS.gene073126.t1.exon2;Parent=MS.gene073126.t1
chr2.2 CDS 13185899 13186110 13185899 ID=cds.MS.gene073126.t1;Parent=MS.gene073126.t1
chr2.2 exon 13187805 13188245 13187805 ID=MS.gene073126.t1.exon3;Parent=MS.gene073126.t1
chr2.2 CDS 13187805 13188245 13187805 ID=cds.MS.gene073126.t1;Parent=MS.gene073126.t1
Gene Sequence

>MS.gene073126

ATGATGGTGGTGCCCTGGCTCGAGAAGTTGCTGACAATCACAACATTTTTCATGGCATGTGAAGTCCACCAAAACGAAGCGAAGAATGAGTGTAACAAGTTTTGCCTTGATTGCAATGGCAATCCATTGTGTGGACCTTGCACGGAAACTCATCACAAAGACCACCGAGTGATCCAAGTCAGTGTTGGTCAATTTGTTAGCTAGATCTATATAATATTTCAATAATCTATCTATCTCTACTTTATACCCGTTTCTATTCATTTTATTTTTCTTACATTCACGCAATTTTGATTATTTTTTTGAATTTGGTATTTTAAATTTGAATCTTGATTCTTGAATTATAAATCAGATAAGGAGATCGTCTTATACTGATGTAGTTAAGACTACTGAAATTTACAAGCATCTTGACATCCTTGGAGTTCAAACTTATGTGATTAGCAACTTTACAGTTGTATTCATAAACAAGAGAGCTCATCTCCAACCTAAGAGAATGAAGATTGGTAAAATTGCTTACTCTAGTGACTCTCTTTGTAAGACATGTGACCGAAATCTCGTAGATTCCACTTACTTTTGCTCTTTGGCATGCAAGGTAGATTCATGACCTCGATCTCATTTTTTTCCCTTGGGGTGTGATAGTCTCGAACCCGGATCACTAAGGTTGAAAAGCCAATTTTCAATCAAGTGACTTCTACAAAAATCGAACACTTTTCCTTTATCATATCACTGATTTTGACTTTGATCAATAAAATTATATTTATCAAAAAGAACTTAATTTAAAACAGTGTTAGGATTAATATCTAGTGAGAATATAATTTTTGTTGATATTTAAATGAGTATTTTGGTAAAAAAAAATAAATAAATAAAAAAATTGGGAGAGTGTTGCTAGATGAGAATAAACTTTCACTTGTTAACATTGAGTTTGTGATTGAAGTTTTCTTTATGTGTCTCCGTGAGCCAATTGGTAGGAATAATGATGTGTAGGGGTCGAAGTTCAAACCCCATACACCGTACTTATTCATATTCAAGAGATAAATTTTTAGTCACTATACTACTTGACCAAAAACAAAAACAAAAAAAGTTTTCTTTATGCCTTTTTTATTTTGTAACAAACATTTTCTTTGAGTTATATAATATTGTTTGTATTATTTATTTGTTGATTTATTTGACTTTTGAAAATAATGGACATTATAGCATACTTTACTTTCTTTCATAACTAATTGTTGTACTTTCGTTTTTTTTTCTTTTTGAAAGAATAATTAATGTTGCATTGTGTGTATATGATACAAGTTTGCATATATAAAAGAGAATGGAGGTTTTTTCTTGAACGAAGAAGAGATGGAAGAAATGAAAAGATTACTGGAGAAATCGATCAAGGTATTAACACCAAAAGAAAAGGCTAAAAAGTTGTGTGAACTAAATCTGAAACGAAAACTAGAAGAAGTAGAAGTTGATACAGAAAATGAAGAGAATGTAGAGGACGAGGATGATGAAGAAGGAGGAGGAGAAAGACAAGAAGACGGAGAAAAAGAAAGTATGCTTCCTGTTAATCGTTCAAATTCAAGAAGAAAAGGAATACCTCATCGGGCAACTTTTTTTTAAGGCCCCAAGTCAAAGGGTTACAGATATTTAAATAATACACTATCGTCTGAGATAGATTTCTAGCTAGATCTACATATATGCTCTTGTAACATTTGATTTCGTCAATATGCTTTACATTTATGCTCTTGTGTTATTAGATTCATTAGACCAATATTTGAACCATGCTTCCCTCAACAAAAAAAAATTGAACCATATGCTATATTTTTTATATCCCGTACATTTATGCTCTTACCCTTGAAATTTATTCATATAGTAATATTATTTCTCTAAAAGGTCAAATGTATTTGGTACACAATTTAGACGAAAAACATTTTACATTTGAATCTCAAAAAGTGAGCAACTATCCTGATCTCTAAAAAAATGTGGTATTTAGTTTGATTTTTAATAGAATAAAAAATGCATGACTTTTTGCTTGAACATGATCGGTTTAAGGATGTCAATGGGTATCCGTGGGTACGGGCAGTATGATATCCGTCCCCGCCCCGTTGGATAAATATGATACCCGTACCCGTCCTCATATTCGTGCGGGTATCTCTATACGGGTAATCCACGGGTATTTAGATACCCACGGATATCCGCGAATACCCATGGATTTTTGATTTTTTTTGTACAAAGAAAAATTCAACTTTTAAAGATCCATACATAACATATTCCATTCAAATTTCATAATCCATACCATTCAAGTTTGAGAAGAAGAAGGGTCATGAGGGACCACCGGTTCAGAGAGAGCATGGAGGACCATCTCAGGCACAGCCCATGCAGGAGGATGCGCCTCAATACCCACCACTACACCCGATGATGCTTGATTACTTGGGAGGTATGGCTTGCTGGGCCCAGGATACATCATCACAGATGTATGTGGACGGTCCTTACTTTGGAGCGGAGCTTTCTCTTGCTGCTGATCAGCACCGTCAGAGGTCCCTTCAGAGCAATGCTTTTGATAGGTTTGGCACTGAAGCCAACATGGAGGACTACATTATGGAACAGAGGAGACGTGCTGGAGAGAGAGAGGGTCGCCTTCGATCCGACTTTGCTCGTGGGAAGGCAGCTAGTGATCAGAGCGTGAATGAGTTTTATGCTGGTTTGCAGGGTGACCCAGAGGCCACTGATGACGATGAGCAGTGA
Protein sequence

>MS.gene073126.t1

MMVVPWLEKLLTITTFFMACEVHQNEAKNECNKFCLDCNGNPLCGPCTETHHKDHRVIQVSVVKTTEIYKHLDILGVQTYVISNFTVVFINKRAHLQPKRMKIGKIAYSSDSLCKTCDRNLVDSTYFCSLACKFEKKKGHEGPPVQREHGGPSQAQPMQEDAPQYPPLHPMMLDYLGGMACWAQDTSSQMYVDGPYFGAELSLAADQHRQRSLQSNAFDRFGTEANMEDYIMEQRRRAGEREGRLRSDFARGKAASDQSVNEFYAGLQGDPEATDDDEQ