AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073152

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073152.t1 MTR_2g084230 91.262 309 20 2 4 311 6 308 0.0 553
MS.gene073152.t1 MTR_2g084230 90.968 310 21 2 4 312 6 309 0.0 549
MS.gene073152.t1 MTR_8g086410 61.111 144 48 3 30 171 46 183 8.24e-44 155
MS.gene073152.t1 MTR_8g086410 61.111 144 48 3 30 171 48 185 8.56e-44 155
MS.gene073152.t1 MTR_8g086410 58.278 151 48 4 30 171 48 192 3.53e-41 148
MS.gene073152.t1 MTR_7g069660 58.015 131 46 2 30 160 15 136 4.81e-41 146
MS.gene073152.t1 MTR_2g016220 48.039 204 85 5 3 205 7 190 1.43e-40 145
MS.gene073152.t1 MTR_2g016220 53.659 164 64 3 3 165 7 159 1.57e-40 145
MS.gene073152.t1 MTR_2g016220 52.381 168 64 4 3 165 7 163 2.03e-38 140
MS.gene073152.t1 MTR_2g023580 57.732 97 41 0 71 167 31 127 6.58e-33 122
MS.gene073152.t1 MTR_0223s0040 63.636 88 27 1 82 169 5 87 1.77e-31 119
MS.gene073152.t1 MTR_1g112370 59.783 92 34 2 81 169 8 99 5.65e-30 116
MS.gene073152.t1 MTR_3g450310 55.696 79 29 1 73 151 3 75 5.26e-23 95.1
MS.gene073152.t1 MTR_1g021520 64.789 71 22 2 87 156 120 188 2.27e-21 92.4
MS.gene073152.t1 MTR_6g071625 68.966 58 18 0 94 151 201 258 2.05e-20 90.9
MS.gene073152.t1 MTR_5g029470 56.962 79 29 1 94 167 218 296 3.89e-20 90.5
MS.gene073152.t1 MTR_3g064840 68.966 58 18 0 94 151 273 330 5.94e-20 90.1
MS.gene073152.t1 MTR_8g066310 55.696 79 30 1 94 167 172 250 7.45e-20 89.0
MS.gene073152.t1 MTR_7g098250 67.857 56 18 0 96 151 263 318 2.91e-19 88.2
MS.gene073152.t1 MTR_7g115530 66.071 56 19 0 96 151 219 274 4.00e-19 86.3
MS.gene073152.t1 MTR_1g080330 54.878 82 35 2 85 165 166 246 6.65e-19 85.5
MS.gene073152.t1 MTR_1g080330 61.194 67 25 1 85 151 166 231 7.93e-19 86.3
MS.gene073152.t1 MTR_7g115530 66.071 56 19 0 96 151 219 274 8.96e-19 85.5
MS.gene073152.t1 MTR_7g115530 66.071 56 19 0 96 151 219 274 1.11e-18 86.3
MS.gene073152.t1 MTR_7g115530 66.071 56 19 0 96 151 219 274 1.24e-18 85.5
MS.gene073152.t1 MTR_7g093030 56.164 73 31 1 91 162 208 280 7.18e-18 82.4
MS.gene073152.t1 MTR_7g093030 56.164 73 31 1 91 162 208 280 2.08e-17 82.4
MS.gene073152.t1 MTR_7g115530 64.286 56 20 0 96 151 219 274 2.69e-17 80.5
MS.gene073152.t1 MTR_7g115530 67.308 52 17 0 96 147 219 270 3.35e-17 80.9
MS.gene073152.t1 MTR_2g086450 53.333 75 33 2 96 168 46 120 3.63e-17 81.6
MS.gene073152.t1 MTR_5g027440 60.345 58 23 0 91 148 21 78 7.59e-17 79.3
MS.gene073152.t1 MTR_6g032990 62.264 53 20 0 96 148 43 95 1.06e-16 80.5
MS.gene073152.t1 MTR_1g053835 58.209 67 27 1 97 162 260 326 2.12e-16 79.7
MS.gene073152.t1 MTR_7g088070 65.000 60 20 1 97 155 52 111 3.95e-16 77.4
MS.gene073152.t1 MTR_7g088070 65.000 60 20 1 97 155 52 111 4.30e-16 77.4
MS.gene073152.t1 MTR_1g053830 48.684 76 38 1 97 171 20 95 4.93e-16 72.8
MS.gene073152.t1 MTR_1g090670 60.714 56 22 0 96 151 45 100 6.84e-16 77.0
MS.gene073152.t1 MTR_2g027800 60.714 56 22 0 96 151 40 95 1.02e-15 76.6
MS.gene073152.t1 MTR_2g027800 62.264 53 20 0 96 148 40 92 1.61e-15 75.9
MS.gene073152.t1 MTR_2g027860 65.455 55 19 0 97 151 51 105 2.75e-15 75.5
MS.gene073152.t1 MTR_5g041350 52.830 53 25 0 96 148 16 68 5.64e-15 75.1
MS.gene073152.t1 MTR_6g444980 55.357 56 25 0 96 151 36 91 3.50e-14 72.8
MS.gene073152.t1 MTR_6g444980 55.357 56 25 0 96 151 36 91 3.86e-14 72.4
MS.gene073152.t1 MTR_1g053800 58.491 53 22 0 97 149 179 231 1.96e-13 70.1
MS.gene073152.t1 MTR_4g081710 52.830 53 25 0 96 148 18 70 2.10e-13 70.1
MS.gene073152.t1 MTR_7g068600 61.017 59 20 1 97 152 15 73 3.21e-13 68.6
MS.gene073152.t1 MTR_7g093010 58.491 53 22 0 97 149 215 267 9.74e-12 65.5
MS.gene073152.t1 MTR_4g021790 43.243 74 38 2 78 148 148 220 1.35e-11 64.3
MS.gene073152.t1 MTR_4g113140 39.130 92 48 1 65 148 197 288 2.76e-11 64.3
MS.gene073152.t1 MTR_4g086835 45.455 66 36 0 83 148 262 327 3.53e-11 63.9
MS.gene073152.t1 MTR_2g450070 52.727 55 25 1 94 148 212 265 3.63e-11 64.3
MS.gene073152.t1 MTR_2g450070 52.727 55 25 1 94 148 212 265 3.65e-11 64.3
MS.gene073152.t1 MTR_4g064730 53.125 64 29 1 85 148 121 183 3.94e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073152.t1 AT2G38300 73.256 86 23 0 75 160 34 119 6.91e-38 137
MS.gene073152.t1 AT2G42660 80.282 71 14 0 87 157 42 112 2.17e-34 126
MS.gene073152.t1 AT2G40260 75.325 77 19 0 78 154 65 141 6.16e-33 125
MS.gene073152.t1 AT4G04605 76.056 71 17 0 87 157 6 76 3.15e-31 117
MS.gene073152.t1 AT2G02060 69.231 78 22 1 71 148 7 82 2.19e-28 110
MS.gene073152.t1 AT2G02060 69.231 78 22 1 71 148 7 82 2.19e-28 110
MS.gene073152.t1 AT1G14600 67.606 71 23 0 87 157 15 85 1.86e-27 108
MS.gene073152.t1 AT4G04555 61.644 73 28 0 83 155 2 74 5.65e-25 97.1
MS.gene073152.t1 AT4G04580 59.155 71 29 0 87 157 6 76 1.04e-23 95.5
MS.gene073152.t1 AT5G42630 65.079 63 22 0 94 156 104 166 7.57e-22 92.0
MS.gene073152.t1 AT5G42630 65.079 63 22 0 94 156 104 166 1.34e-21 92.8
MS.gene073152.t1 AT1G32240 56.962 79 29 1 94 167 212 290 1.78e-20 90.9
MS.gene073152.t1 AT1G32240 56.962 79 29 1 94 167 212 290 2.41e-20 90.9
MS.gene073152.t1 AT4G17695 62.319 69 24 1 86 154 157 223 3.50e-20 89.7
MS.gene073152.t1 AT5G16560 68.966 58 18 0 94 151 218 275 4.75e-20 90.1
MS.gene073152.t1 AT5G29000 53.947 76 33 1 76 151 214 287 7.98e-18 82.8
MS.gene073152.t1 AT5G29000 53.247 77 34 1 75 151 170 244 1.34e-17 82.8
MS.gene073152.t1 AT5G29000 53.247 77 34 1 75 151 170 244 1.34e-17 82.8
MS.gene073152.t1 AT5G29000 53.247 77 34 1 75 151 213 287 1.52e-17 82.8
MS.gene073152.t1 AT4G28610 65.455 55 19 0 97 151 227 281 1.66e-17 82.8
MS.gene073152.t1 AT3G04450 61.818 55 21 0 97 151 240 294 2.08e-17 81.6
MS.gene073152.t1 AT5G45580 60.345 58 23 0 91 148 3 60 2.51e-17 80.5
MS.gene073152.t1 AT3G04450 61.818 55 21 0 97 151 200 254 3.16e-17 82.0
MS.gene073152.t1 AT3G04450 61.818 55 21 0 97 151 200 254 3.16e-17 82.0
MS.gene073152.t1 AT3G04450 61.818 55 21 0 97 151 240 294 3.18e-17 82.0
MS.gene073152.t1 AT5G45580 60.345 58 23 0 91 148 19 76 3.40e-17 80.5
MS.gene073152.t1 AT5G18240 48.276 87 42 2 80 165 30 114 8.86e-17 80.5
MS.gene073152.t1 AT5G18240 48.276 87 42 2 80 165 30 114 8.86e-17 80.5
MS.gene073152.t1 AT5G18240 48.276 87 42 2 80 165 30 114 1.02e-16 80.5
MS.gene073152.t1 AT5G18240 48.276 87 42 2 80 165 30 114 1.12e-16 80.5
MS.gene073152.t1 AT5G18240 48.276 87 42 2 80 165 30 114 1.12e-16 80.5
MS.gene073152.t1 AT3G13040 67.273 55 18 0 97 151 243 297 1.21e-16 80.5
MS.gene073152.t1 AT3G13040 67.273 55 18 0 97 151 243 297 1.21e-16 80.5
MS.gene073152.t1 AT3G13040 67.273 55 18 0 97 151 243 297 1.21e-16 80.5
MS.gene073152.t1 AT3G12730 38.017 121 65 3 96 216 24 134 4.05e-16 76.6
MS.gene073152.t1 AT2G06020 42.708 96 52 1 78 170 66 161 4.57e-16 77.8
MS.gene073152.t1 AT3G04030 55.357 56 25 0 96 151 46 101 6.74e-16 77.8
MS.gene073152.t1 AT3G04030 55.357 56 25 0 96 151 46 101 7.85e-16 77.8
MS.gene073152.t1 AT2G01060 46.939 98 39 2 97 181 17 114 9.17e-16 76.6
MS.gene073152.t1 AT5G06800 56.452 62 26 1 97 157 194 255 4.16e-15 74.7
MS.gene073152.t1 AT2G20400 63.636 55 20 0 97 151 233 287 4.46e-15 75.5
MS.gene073152.t1 AT2G20400 63.636 55 20 0 97 151 233 287 4.52e-15 75.5
MS.gene073152.t1 AT3G24120 56.604 53 23 0 96 148 42 94 1.23e-14 73.6
MS.gene073152.t1 AT3G24120 56.604 53 23 0 96 148 42 94 1.24e-14 73.6
MS.gene073152.t1 AT4G13640 56.604 53 23 0 96 148 38 90 1.31e-14 73.2
MS.gene073152.t1 AT4G13640 56.604 53 23 0 96 148 38 90 1.36e-14 73.2
MS.gene073152.t1 AT5G06800 56.452 62 26 1 97 157 194 255 2.13e-14 73.6
MS.gene073152.t1 AT5G06800 56.452 62 26 1 97 157 194 255 2.16e-14 73.6
MS.gene073152.t1 AT1G79430 55.357 56 25 0 96 151 35 90 2.22e-14 73.2
MS.gene073152.t1 AT1G69580 54.717 53 24 0 96 148 35 87 1.50e-13 70.9
MS.gene073152.t1 AT1G69580 54.717 53 24 0 96 148 35 87 1.66e-13 70.5
MS.gene073152.t1 AT3G04030 53.571 56 25 1 96 151 46 100 1.98e-13 70.9
MS.gene073152.t1 AT5G59570 57.143 56 23 1 93 148 139 193 1.38e-11 64.7
MS.gene073152.t1 AT5G59570 57.143 56 23 1 93 148 139 193 1.38e-11 64.7
MS.gene073152.t1 AT2G03500 50.746 67 31 1 82 148 222 286 2.96e-11 64.3
MS.gene073152.t1 AT3G62670 44.872 78 38 2 76 148 191 268 3.14e-11 64.3
MS.gene073152.t1 AT5G05090 48.649 74 37 1 75 148 61 133 8.67e-11 62.0
MS.gene073152.t1 AT4G37180 50.847 59 29 0 90 148 205 263 9.21e-11 62.4
MS.gene073152.t1 AT4G31920 37.255 102 63 1 83 184 171 271 9.58e-11 62.8

Find 60 sgRNAs with CRISPR-Local

Find 151 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCACTGAGTACACAATATT+TGG 0.169202 2.2:-13516106 MS.gene073152:CDS
CTTACTTGGCTAGACCTAAT+AGG 0.308735 2.2:+13516247 None:intergenic
GCAAGGAACGCATCACATTA+TGG 0.355879 2.2:-13516980 MS.gene073152:CDS
CGGGTAGTAATTGACTTACT+TGG 0.374141 2.2:+13516233 None:intergenic
TTCACATCTATTTGATGTAA+AGG 0.385780 2.2:-13516296 MS.gene073152:CDS
TCAAAGCTCATGACACTTCA+AGG 0.388453 2.2:-13516858 MS.gene073152:intron
TATGATGGTTGTTTCAATAG+AGG 0.404012 2.2:+13516889 None:intergenic
GTCCTCTCACATTCATCAAC+TGG 0.411306 2.2:+13517454 None:intergenic
ACATGTGAAGAGTCATTTGC+AGG 0.413814 2.2:-13517421 MS.gene073152:intron
GAAATAATATTGCACCTATT+AGG 0.414171 2.2:-13516261 MS.gene073152:CDS
CATCAACTGGAGAACCAACT+TGG 0.425229 2.2:+13517467 None:intergenic
AATAATAATTCTAGTGAAGA+AGG 0.443311 2.2:-13518298 MS.gene073152:CDS
TTATCTCAAGGGGATGATAA+TGG 0.444178 2.2:-13516199 MS.gene073152:CDS
CATCACATTATGGATATGTA+TGG 0.453888 2.2:-13516970 MS.gene073152:CDS
GTGCATGCTGTTGAGAGGCT+TGG 0.454830 2.2:-13518190 MS.gene073152:CDS
AAACCGGCCTCTTGGCAGAT+TGG 0.455960 2.2:-13518457 MS.gene073152:CDS
ACAAAAGATAAATGAAGATC+AGG 0.462114 2.2:+13518211 None:intergenic
TCGGAGAGAAACCGGCCTCT+TGG 0.463390 2.2:-13518465 MS.gene073152:CDS
TTTCAACACCAAAGTGTCCT+TGG 0.465491 2.2:+13516935 None:intergenic
TAGCGGCATCGGAGAGAAAC+CGG 0.466371 2.2:-13518473 MS.gene073152:CDS
AGTGAAAGTAGTAGCGGCAT+CGG 0.474860 2.2:-13518484 MS.gene073152:CDS
AATTCTAGTGAAGAAGGAAA+AGG 0.475804 2.2:-13518292 MS.gene073152:CDS
TTTGTAGATTTCATGAGAGT+GGG 0.483163 2.2:-13516375 MS.gene073152:intron
CTTCCAATCTGCCAAGAGGC+CGG 0.484626 2.2:+13518454 None:intergenic
TTCAATGTCCAAGGACACTT+TGG 0.487141 2.2:-13516943 MS.gene073152:CDS
CATGCTGTTGAGAGGCTTGG+AGG 0.487742 2.2:-13518187 MS.gene073152:CDS
AATGAAGATGCCGTGGATGA+TGG 0.491306 2.2:-13518409 MS.gene073152:CDS
CGAAAATGCCTAGGCTTCGT+TGG 0.491348 2.2:-13518237 MS.gene073152:CDS
CATCACAAGAAGGGAATTTG+AGG 0.500720 2.2:-13518324 MS.gene073152:CDS
ATAATTGATAGAACTAGAAA+AGG 0.502223 2.2:+13516153 None:intergenic
ATTCTAGTGAAGAAGGAAAA+GGG 0.515576 2.2:-13518291 MS.gene073152:CDS
AGGAAAAGGGCGTGGAAGTA+CGG 0.529396 2.2:-13518278 MS.gene073152:CDS
ATTGTGTACTCAGTGAAGAT+AGG 0.533099 2.2:+13516112 None:intergenic
GAACTAGAAAAGGTGCTCCA+TGG 0.547694 2.2:+13516163 None:intergenic
TCACTCTTGACATCTTTGCT+AGG 0.549974 2.2:+13518502 None:intergenic
GCTTTGATTTCATTATATGA+TGG 0.554601 2.2:+13516874 None:intergenic
TCAGGAGTCCAACGAAGCCT+AGG 0.559435 2.2:+13518229 None:intergenic
AATGAGATATCATCACAAGA+AGG 0.560760 2.2:-13518334 MS.gene073152:CDS
GTACACAATATTTGGTTCTG+AGG 0.563826 2.2:-13516098 MS.gene073152:CDS
TATGGAAGATTCAATGTCCA+AGG 0.583731 2.2:-13516952 MS.gene073152:CDS
TGTAGGAGCAACACCCAAGT+TGG 0.585790 2.2:-13517481 MS.gene073152:intron
ATGAGATATCATCACAAGAA+GGG 0.586610 2.2:-13518333 MS.gene073152:CDS
AGTGAAGAAGGAAAAGGGCG+TGG 0.587852 2.2:-13518286 MS.gene073152:CDS
CGATTTAAATGAAGATGCCG+TGG 0.592934 2.2:-13518416 MS.gene073152:CDS
ATCAACTGGAGAACCAACTT+GGG 0.599898 2.2:+13517468 None:intergenic
CTTCAGATCAAGTTCAGTGT+GGG 0.604465 2.2:-13516329 MS.gene073152:CDS
ATGTGAGGTCGAAAATGCCT+AGG 0.605149 2.2:-13518246 MS.gene073152:CDS
TTGTTGCTTATTTCACCACA+AGG 0.610333 2.2:+13518355 None:intergenic
ATGCTGTTGAGAGGCTTGGA+GGG 0.612873 2.2:-13518186 MS.gene073152:CDS
GTCAAGAGTGAAAGTAGTAG+CGG 0.620238 2.2:-13518490 MS.gene073152:CDS
CTCCAGTTGATGAATGTGAG+AGG 0.629384 2.2:-13517456 MS.gene073152:CDS
ATGATCATCTCCATCATCCA+CGG 0.631691 2.2:+13518399 None:intergenic
AAGCTTGATGAGTCTGGCCA+AGG 0.633707 2.2:-13517122 MS.gene073152:intron
CTGATAAGTGAAGGCCCTTG+TGG 0.637131 2.2:-13518370 MS.gene073152:CDS
TGTTTCAATAGAGGAGACGA+TGG 0.646679 2.2:+13516898 None:intergenic
GTTTCAATAGAGGAGACGAT+GGG 0.648257 2.2:+13516899 None:intergenic
TGTTGCTTATTTCACCACAA+GGG 0.657001 2.2:+13518356 None:intergenic
TCTTCAGATCAAGTTCAGTG+TGG 0.702689 2.2:-13516330 MS.gene073152:CDS
CATGTGATACTGATAAGTGA+AGG 0.715293 2.2:-13518379 MS.gene073152:CDS
GTACGGTTAGACAATATGTG+AGG 0.731117 2.2:-13518261 MS.gene073152:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAAAGAAAAATATGTAA+TGG + chr2.2:13518121-13518140 None:intergenic 10.0%
!!! TTTTATTTTCAATTCATTTT+TGG - chr2.2:13517317-13517336 MS.gene073152:intron 10.0%
!! ACATTAAATAATTAAGTGTT+GGG + chr2.2:13517942-13517961 None:intergenic 15.0%
!!! AAATAGAAACATTTAAAACA+TGG - chr2.2:13518166-13518185 MS.gene073152:intron 15.0%
!!! CTTATATAACGTTTTAATTA+TGG - chr2.2:13517424-13517443 MS.gene073152:CDS 15.0%
!! AAAAATGAAAAAACAGTTGA+AGG + chr2.2:13516988-13517007 None:intergenic 20.0%
!! AACAAATAGATCAAAGAATA+AGG + chr2.2:13517068-13517087 None:intergenic 20.0%
!! AATAATAATTCTAGTGAAGA+AGG - chr2.2:13516303-13516322 MS.gene073152:CDS 20.0%
!! ACAAATAGATCAAAGAATAA+GGG + chr2.2:13517067-13517086 None:intergenic 20.0%
!! AGTAATAATGCAGAAATATT+TGG + chr2.2:13518071-13518090 None:intergenic 20.0%
!! ATAATTGATAGAACTAGAAA+AGG + chr2.2:13518451-13518470 None:intergenic 20.0%
!! GACATTAAATAATTAAGTGT+TGG + chr2.2:13517943-13517962 None:intergenic 20.0%
!! TAACAATATTGGATATATGT+AGG - chr2.2:13517103-13517122 MS.gene073152:intron 20.0%
!! TTATCTAATTTCATTACCTT+TGG - chr2.2:13516502-13516521 MS.gene073152:intron 20.0%
!! TTTGGTCATCATATTATTAT+AGG + chr2.2:13516960-13516979 None:intergenic 20.0%
!!! CGTTTTAATTATGGTTTTTT+TGG - chr2.2:13517433-13517452 MS.gene073152:CDS 20.0%
!!! TGATTTTGCTAACAATATTA+AGG + chr2.2:13517517-13517536 None:intergenic 20.0%
!!! TTAGTGGATAATTTCTTAAT+AGG - chr2.2:13516825-13516844 MS.gene073152:intron 20.0%
!!! TTTAATTATGGTTTTTTTGG+AGG - chr2.2:13517436-13517455 MS.gene073152:CDS 20.0%
!!! TTTTTATAGACAAATGTTAG+TGG - chr2.2:13516809-13516828 MS.gene073152:intron 20.0%
! AATCAAAAAGCTTATCTCAA+GGG - chr2.2:13518391-13518410 MS.gene073152:CDS 25.0%
! ACAAAAGATAAATGAAGATC+AGG + chr2.2:13516393-13516412 None:intergenic 25.0%
! CAAACATCAAATATGCTAAA+TGG - chr2.2:13516584-13516603 MS.gene073152:intron 25.0%
! CAAATAGATCAAAGAATAAG+GGG + chr2.2:13517066-13517085 None:intergenic 25.0%
! GAAATAATATTGCACCTATT+AGG - chr2.2:13518340-13518359 MS.gene073152:CDS 25.0%
! TAAATTTGCATAGTGTAATG+TGG - chr2.2:13516476-13516495 MS.gene073152:intron 25.0%
! TTATAGGTATGATCCAAAAA+TGG + chr2.2:13516944-13516963 None:intergenic 25.0%
! TTCACATCTATTTGATGTAA+AGG - chr2.2:13518305-13518324 MS.gene073152:CDS 25.0%
!! AAGAAAAAGTACATTCCAAA+AGG + chr2.2:13517378-13517397 None:intergenic 25.0%
!! AATTTTACCTTCAACAATAC+CGG - chr2.2:13518012-13518031 MS.gene073152:intron 25.0%
!! GCTTTGATTTCATTATATGA+TGG + chr2.2:13517730-13517749 None:intergenic 25.0%
!!! CTTTGATCTATTTGTTTTGA+GGG - chr2.2:13517072-13517091 MS.gene073152:intron 25.0%
!!! TCTTTGATCTATTTGTTTTG+AGG - chr2.2:13517071-13517090 MS.gene073152:intron 25.0%
!!! TTGAGATAAGCTTTTTGATT+CGG + chr2.2:13518391-13518410 None:intergenic 25.0%
AATGAGATATCATCACAAGA+AGG - chr2.2:13516267-13516286 MS.gene073152:CDS 30.0%
AATTCTAGTGAAGAAGGAAA+AGG - chr2.2:13516309-13516328 MS.gene073152:CDS 30.0%
ATCAAAAAGCTTATCTCAAG+GGG - chr2.2:13518392-13518411 MS.gene073152:CDS 30.0%
ATGAGATATCATCACAAGAA+GGG - chr2.2:13516268-13516287 MS.gene073152:CDS 30.0%
ATTCTAGTGAAGAAGGAAAA+GGG - chr2.2:13516310-13516329 MS.gene073152:CDS 30.0%
CATCACATTATGGATATGTA+TGG - chr2.2:13517631-13517650 MS.gene073152:intron 30.0%
GAATCAAAAAGCTTATCTCA+AGG - chr2.2:13518390-13518409 MS.gene073152:CDS 30.0%
GTAGTTCATAGGTATGATTT+GGG + chr2.2:13516912-13516931 None:intergenic 30.0%
GTTATGAGTTGTGTATTTGT+TGG - chr2.2:13516540-13516559 MS.gene073152:intron 30.0%
TGTAGTTCATAGGTATGATT+TGG + chr2.2:13516913-13516932 None:intergenic 30.0%
TTCACTGAGTACACAATATT+TGG - chr2.2:13518495-13518514 MS.gene073152:CDS 30.0%
TTTGTAGATTTCATGAGAGT+GGG - chr2.2:13518226-13518245 MS.gene073152:CDS 30.0%
! AAACAGTTGAAGGTATGATT+TGG + chr2.2:13516978-13516997 None:intergenic 30.0%
! AGCAAAATCATGTTTTTCCA+AGG - chr2.2:13517527-13517546 MS.gene073152:intron 30.0%
! ATTGCGTGTGTAATTTGTAA+TGG - chr2.2:13517272-13517291 MS.gene073152:intron 30.0%
! CTACATCTTCAAACCATTTT+TGG - chr2.2:13516928-13516947 MS.gene073152:CDS 30.0%
! GCAAAATCATGTTTTTCCAA+GGG - chr2.2:13517528-13517547 MS.gene073152:intron 30.0%
! TAACACCTTTAGATTTTGAG+TGG - chr2.2:13516632-13516651 MS.gene073152:intron 30.0%
! TATGATGGTTGTTTCAATAG+AGG + chr2.2:13517715-13517734 None:intergenic 30.0%
! TTGCGTGTGTAATTTGTAAT+GGG - chr2.2:13517273-13517292 MS.gene073152:intron 30.0%
! TTTTGTAGATTTCATGAGAG+TGG - chr2.2:13518225-13518244 MS.gene073152:CDS 30.0%
!! ACTCAAAATCTAAAGGTGTT+AGG + chr2.2:13516633-13516652 None:intergenic 30.0%
!! CAACTCATAACTTGTTTCAT+TGG + chr2.2:13516531-13516550 None:intergenic 30.0%
!! TAAGGTTCTGAAATATACCT+TGG + chr2.2:13517499-13517518 None:intergenic 30.0%
!! TGAGATAAGCTTTTTGATTC+GGG + chr2.2:13518390-13518409 None:intergenic 30.0%
!!! CTATTTGTTTTGAGGGTTTT+TGG - chr2.2:13517079-13517098 MS.gene073152:intron 30.0%
!!! GGGTTTTTGGTTAACAATAT+TGG - chr2.2:13517092-13517111 MS.gene073152:intron 30.0%
ACACTCCACTCAAAATCTAA+AGG + chr2.2:13516640-13516659 None:intergenic 35.0%
AGTAAAAAGCTTGATGAGTC+TGG - chr2.2:13517473-13517492 MS.gene073152:CDS 35.0%
ATTGTGTACTCAGTGAAGAT+AGG + chr2.2:13518492-13518511 None:intergenic 35.0%
CATGTGATACTGATAAGTGA+AGG - chr2.2:13516222-13516241 MS.gene073152:CDS 35.0%
CATGTGATGTATAAAACCCT+TGG + chr2.2:13517547-13517566 None:intergenic 35.0%
CTCTGAATTCTATCGTGATT+AGG - chr2.2:13516747-13516766 MS.gene073152:intron 35.0%
GATTAGGAAGTAGTTCTTTC+CGG - chr2.2:13516763-13516782 MS.gene073152:intron 35.0%
GGTTTGAAGATGTAGTTCAT+AGG + chr2.2:13516923-13516942 None:intergenic 35.0%
GTATCTCTGTCTTGTAATCT+TGG - chr2.2:13516669-13516688 MS.gene073152:intron 35.0%
TATGGAAGATTCAATGTCCA+AGG - chr2.2:13517649-13517668 MS.gene073152:intron 35.0%
TTATCTCAAGGGGATGATAA+TGG - chr2.2:13518402-13518421 MS.gene073152:CDS 35.0%
TTCAGAGATCAATATTGTCG+AGG + chr2.2:13516734-13516753 None:intergenic 35.0%
! ACTCATCAAGCTTTTTACTC+CGG + chr2.2:13517472-13517491 None:intergenic 35.0%
! AGTTCAGAAACATGTCCTTT+TGG - chr2.2:13517360-13517379 MS.gene073152:intron 35.0%
! ATGGTGCAAAACATTTTCCA+TGG - chr2.2:13518421-13518440 MS.gene073152:CDS 35.0%
! TGTTGCTTATTTCACCACAA+GGG + chr2.2:13516248-13516267 None:intergenic 35.0%
! TTGTTGCTTATTTCACCACA+AGG + chr2.2:13516249-13516268 None:intergenic 35.0%
!! GTACACAATATTTGGTTCTG+AGG - chr2.2:13518503-13518522 MS.gene073152:CDS 35.0%
!! GTTGAAGGAGTATCAAGAAT+AGG + chr2.2:13516887-13516906 None:intergenic 35.0%
!! TCCTAACCCTAAAGTTTTGT+TGG - chr2.2:13517905-13517924 MS.gene073152:intron 35.0%
!! TTTAGATTTTGAGTGGAGTG+TGG - chr2.2:13516639-13516658 MS.gene073152:intron 35.0%
!! TTTGTACCCTACAGTTTTGT+AGG + chr2.2:13518041-13518060 None:intergenic 35.0%
!!! CCTAACCCTAAAGTTTTGTT+GGG - chr2.2:13517906-13517925 MS.gene073152:intron 35.0%
!!! TCCTTGCGTTCTATTTTTGT+GGG + chr2.2:13517608-13517627 None:intergenic 35.0%
!!! TTCCTTGCGTTCTATTTTTG+TGG + chr2.2:13517609-13517628 None:intergenic 35.0%
ACATGTGAAGAGTCATTTGC+AGG - chr2.2:13517180-13517199 MS.gene073152:intron 40.0%
ATCAACTGGAGAACCAACTT+GGG + chr2.2:13517136-13517155 None:intergenic 40.0%
ATGATCATCTCCATCATCCA+CGG + chr2.2:13516205-13516224 None:intergenic 40.0%
ATTGTCGAGGAGTTCAGTAA+AGG + chr2.2:13516721-13516740 None:intergenic 40.0%
CATCACAAGAAGGGAATTTG+AGG - chr2.2:13516277-13516296 MS.gene073152:CDS 40.0%
CGATTTAAATGAAGATGCCG+TGG - chr2.2:13516185-13516204 MS.gene073152:CDS 40.0%
CTACGCCCAACAAAACTTTA+GGG + chr2.2:13517914-13517933 None:intergenic 40.0%
CTTACTTGGCTAGACCTAAT+AGG + chr2.2:13518357-13518376 None:intergenic 40.0%
CTTCAGATCAAGTTCAGTGT+GGG - chr2.2:13518272-13518291 MS.gene073152:CDS 40.0%
GAGTATCAAGAATAGGTCAG+GGG + chr2.2:13516880-13516899 None:intergenic 40.0%
GTACGGTTAGACAATATGTG+AGG - chr2.2:13516340-13516359 MS.gene073152:CDS 40.0%
GTCAAGAGTGAAAGTAGTAG+CGG - chr2.2:13516111-13516130 MS.gene073152:CDS 40.0%
GTTTCAATAGAGGAGACGAT+GGG + chr2.2:13517705-13517724 None:intergenic 40.0%
TCAAAGCTCATGACACTTCA+AGG - chr2.2:13517743-13517762 MS.gene073152:intron 40.0%
TCACTCTTGACATCTTTGCT+AGG + chr2.2:13516102-13516121 None:intergenic 40.0%
TCCCACAAAAATAGAACGCA+AGG - chr2.2:13517604-13517623 MS.gene073152:intron 40.0%
TCTTCAGATCAAGTTCAGTG+TGG - chr2.2:13518271-13518290 MS.gene073152:CDS 40.0%
TGTTTCAATAGAGGAGACGA+TGG + chr2.2:13517706-13517725 None:intergenic 40.0%
TTCAATGTCCAAGGACACTT+TGG - chr2.2:13517658-13517677 MS.gene073152:intron 40.0%
TTGTCGAGGAGTTCAGTAAA+GGG + chr2.2:13516720-13516739 None:intergenic 40.0%
! CGGGTAGTAATTGACTTACT+TGG + chr2.2:13518371-13518390 None:intergenic 40.0%
! GGAGTATCAAGAATAGGTCA+GGG + chr2.2:13516881-13516900 None:intergenic 40.0%
! TTTCAACACCAAAGTGTCCT+TGG + chr2.2:13517669-13517688 None:intergenic 40.0%
! TTTTCTTCCAATCTGCCAAG+AGG + chr2.2:13516154-13516173 None:intergenic 40.0%
!! AGGAGTATCAAGAATAGGTC+AGG + chr2.2:13516882-13516901 None:intergenic 40.0%
!! CCCAACAAAACTTTAGGGTT+AGG + chr2.2:13517909-13517928 None:intergenic 40.0%
!! TTTTGGAGGTTCAGATGTAC+CGG - chr2.2:13517450-13517469 MS.gene073152:CDS 40.0%
AATGAAGATGCCGTGGATGA+TGG - chr2.2:13516192-13516211 MS.gene073152:CDS 45.0%
AGTGAAAGTAGTAGCGGCAT+CGG - chr2.2:13516117-13516136 MS.gene073152:CDS 45.0%
ATGTGAGGTCGAAAATGCCT+AGG - chr2.2:13516355-13516374 MS.gene073152:CDS 45.0%
CATCAACTGGAGAACCAACT+TGG + chr2.2:13517137-13517156 None:intergenic 45.0%
CCATGTACGTTGAGTGATGT+GGG - chr2.2:13517837-13517856 MS.gene073152:intron 45.0%
CCTACGCCCAACAAAACTTT+AGG + chr2.2:13517915-13517934 None:intergenic 45.0%
CGGTGTCCTACAAAACTGTA+GGG - chr2.2:13518032-13518051 MS.gene073152:intron 45.0%
CTTGTTTCATTGGTCGCCAA+AGG + chr2.2:13516521-13516540 None:intergenic 45.0%
GCAAGGAACGCATCACATTA+TGG - chr2.2:13517621-13517640 MS.gene073152:intron 45.0%
GTCCTCTCACATTCATCAAC+TGG + chr2.2:13517150-13517169 None:intergenic 45.0%
TAGGACACCGGTATTGTTGA+AGG + chr2.2:13518022-13518041 None:intergenic 45.0%
TCCATGTACGTTGAGTGATG+TGG - chr2.2:13517836-13517855 MS.gene073152:intron 45.0%
TGGAGGGCAAGAAAGTAAGT+GGG - chr2.2:13516431-13516450 MS.gene073152:intron 45.0%
TTGGAGGGCAAGAAAGTAAG+TGG - chr2.2:13516430-13516449 MS.gene073152:intron 45.0%
! CTTTTGTGCATGCTGTTGAG+AGG - chr2.2:13516406-13516425 MS.gene073152:intron 45.0%
! GGTATGATTTGGGACGTTGA+AGG + chr2.2:13516902-13516921 None:intergenic 45.0%
!! CCTACAGTTTTGTAGGACAC+CGG + chr2.2:13518034-13518053 None:intergenic 45.0%
!! CTCCAGTTGATGAATGTGAG+AGG - chr2.2:13517145-13517164 MS.gene073152:intron 45.0%
!! GAACTAGAAAAGGTGCTCCA+TGG + chr2.2:13518441-13518460 None:intergenic 45.0%
!!! CCTAAAGTTTTGTTGGGCGT+AGG - chr2.2:13517912-13517931 MS.gene073152:intron 45.0%
AAGCTTGATGAGTCTGGCCA+AGG - chr2.2:13517479-13517498 MS.gene073152:CDS 50.0%
AGTGAAGAAGGAAAAGGGCG+TGG - chr2.2:13516315-13516334 MS.gene073152:CDS 50.0%
ATGCTGTTGAGAGGCTTGGA+GGG - chr2.2:13516415-13516434 MS.gene073152:intron 50.0%
CCCACATCACTCAACGTACA+TGG + chr2.2:13517840-13517859 None:intergenic 50.0%
CCGGTGTCCTACAAAACTGT+AGG - chr2.2:13518031-13518050 MS.gene073152:intron 50.0%
CGAAAATGCCTAGGCTTCGT+TGG - chr2.2:13516364-13516383 MS.gene073152:CDS 50.0%
CTGATAAGTGAAGGCCCTTG+TGG - chr2.2:13516231-13516250 MS.gene073152:CDS 50.0%
TGTAGGAGCAACACCCAAGT+TGG - chr2.2:13517120-13517139 MS.gene073152:intron 50.0%
! AGGAAAAGGGCGTGGAAGTA+CGG - chr2.2:13516323-13516342 MS.gene073152:CDS 50.0%
! AGTAGTTCTTTCCGGTGTCG+TGG - chr2.2:13516771-13516790 MS.gene073152:intron 50.0%
AAACCGGCCTCTTGGCAGAT+TGG - chr2.2:13516144-13516163 MS.gene073152:CDS 55.0%
ACGCACACAAACCACGACAC+CGG + chr2.2:13516785-13516804 None:intergenic 55.0%
CATGCTGTTGAGAGGCTTGG+AGG - chr2.2:13516414-13516433 MS.gene073152:intron 55.0%
CTTCCAATCTGCCAAGAGGC+CGG + chr2.2:13516150-13516169 None:intergenic 55.0%
GTGCATGCTGTTGAGAGGCT+TGG - chr2.2:13516411-13516430 MS.gene073152:intron 55.0%
TCAGGAGTCCAACGAAGCCT+AGG + chr2.2:13516375-13516394 None:intergenic 55.0%
! TAGCGGCATCGGAGAGAAAC+CGG - chr2.2:13516128-13516147 MS.gene073152:CDS 55.0%
TCGGAGAGAAACCGGCCTCT+TGG - chr2.2:13516136-13516155 MS.gene073152:CDS 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 13516087 13518536 13516087 ID=MS.gene073152
chr2.2 mRNA 13516087 13518536 13516087 ID=MS.gene073152.t1;Parent=MS.gene073152
chr2.2 exon 13518179 13518536 13518179 ID=MS.gene073152.t1.exon1;Parent=MS.gene073152.t1
chr2.2 CDS 13518179 13518536 13518179 ID=cds.MS.gene073152.t1;Parent=MS.gene073152.t1
chr2.2 exon 13517422 13517498 13517422 ID=MS.gene073152.t1.exon2;Parent=MS.gene073152.t1
chr2.2 CDS 13517422 13517498 13517422 ID=cds.MS.gene073152.t1;Parent=MS.gene073152.t1
chr2.2 exon 13517123 13517159 13517123 ID=MS.gene073152.t1.exon3;Parent=MS.gene073152.t1
chr2.2 CDS 13517123 13517159 13517123 ID=cds.MS.gene073152.t1;Parent=MS.gene073152.t1
chr2.2 exon 13516859 13517024 13516859 ID=MS.gene073152.t1.exon4;Parent=MS.gene073152.t1
chr2.2 CDS 13516859 13517024 13516859 ID=cds.MS.gene073152.t1;Parent=MS.gene073152.t1
chr2.2 exon 13516087 13516390 13516087 ID=MS.gene073152.t1.exon5;Parent=MS.gene073152.t1
chr2.2 CDS 13516087 13516390 13516087 ID=cds.MS.gene073152.t1;Parent=MS.gene073152.t1
Gene Sequence

>MS.gene073152

ATGGGGGAAATTCCTAGCAAAGATGTCAAGAGTGAAAGTAGTAGCGGCATCGGAGAGAAACCGGCCTCTTGGCAGATTGGAAGAAAACGATCATTCTTCGATTTAAATGAAGATGCCGTGGATGATGGAGATGATCATGTGATACTGATAAGTGAAGGCCCTTGTGGTGAAATAAGCAACAATGAGATATCATCACAAGAAGGGAATTTGAGGAGCAATAATAATTCTAGTGAAGAAGGAAAAGGGCGTGGAAGTACGGTTAGACAATATGTGAGGTCGAAAATGCCTAGGCTTCGTTGGACTCCTGATCTTCATTTATCTTTTGTGCATGCTGTTGAGAGGCTTGGAGGGCAAGAAAGTAAGTGGGACTCATTTCAATCTTATGAACATAAATTTGCATAGTGTAATGTGGATTTTATCTAATTTCATTACCTTTGGCGACCAATGAAACAAGTTATGAGTTGTGTATTTGTTGGACTCAAGTGTGAGTGACTAGTCAAACATCAAATATGCTAAATGGTTGATACAGAGATAACTCATATACCTAACACCTTTAGATTTTGAGTGGAGTGTGGAGATGTTGTATCTCTGTCTTGTAATCTTGGAACATTGATAATTTGTTGTTCACGACCCTTTACTGAACTCCTCGACAATATTGATCTCTGAATTCTATCGTGATTAGGAAGTAGTTCTTTCCGGTGTCGTGGTTTGTGTGCGTGTATTTTTTATAGACAAATGTTAGTGGATAATTTCTTAATAGGAGAGAAAATTTGTTAGCAACATAATTGATCCCCTGACCTATTCTTGATACTCCTTCAACGTCCCAAATCATACCTATGAACTACATCTTCAAACCATTTTTGGATCATACCTATAATAATATGATGACCAAATCATACCTTCAACTGTTTTTTCATTTTTGTTTTTTCAGTTTATATGAATTGAAGTACTTCTTATACTTATTTAGATATAGACGCCCCTTATTCTTTGATCTATTTGTTTTGAGGGTTTTTGGTTAACAATATTGGATATATGTAGGAGCAACACCCAAGTTGGTTCTCCAGTTGATGAATGTGAGAGGACTTAGCATTGCACATGTGAAGAGTCATTTGCAGGTAATATATATTCTTGTATATAAGTTGATTTATGCACAAAATTGATGTTTCTTAATTTATTGTTTGATTAATTGCGTGTGTAATTTGTAATGGGATAGAGTACATCTTTCATTCTTTTTATTTTCAATTCATTTTTGGTTATAAAAAAAATTGTGTCAAGTTCAGAAACATGTCCTTTTGGAATGTACTTTTTCTTTCTTTCTTTCTTTCTATTCTGTCTTTCTTATATAACGTTTTAATTATGGTTTTTTTGGAGGTTCAGATGTACCGGAGTAAAAAGCTTGATGAGTCTGGCCAAGGTATATTTCAGAACCTTAATATTGTTAGCAAAATCATGTTTTTCCAAGGGTTTTATACATCACATGCATGTTTTATATTCATTTTTGTTTTATTTCAGTTTTGTCCCACAAAAATAGAACGCAAGGAACGCATCACATTATGGATATGTATGGAAGATTCAATGTCCAAGGACACTTTGGTGTTGAAAACAACTATTTGCACCCATCGTCTCCTCTATTGAAACAACCATCATATAATGAAATCAAAGCTCATGACACTTCAAGGTATAGTATAGAAAAAACTTTCAGATTATTTTATTCTTATGCACTACAAATGTAAAAAGTAAAATAAAATTTCCATGTACGTTGAGTGATGTGGGACACAAACACAATGCATCATTAAATATGTGTAAAATTATAATACTTCCTAACCCTAAAGTTTTGTTGGGCGTAGGATATCCCAACACTTAATTATTTAATGTCATATCATTTTTTTACATTAACTTATTTTAAAAAAAAAATGTTTTAAATTAATTTTACCTTCAACAATACCGGTGTCCTACAAAACTGTAGGGTACAAAGAATTTTCCAAATATTTCTGCATTATTACTATTCTAAAAGAATACATACAATGAAAACCATTACATATTTTTCTTTTATAATTATTATAATGAAAATTTTAAATAAAATAGAAACATTTAAAACATGGATGTGAAATTAATTTTGATAATTCTTTAATTGTTAATTTTGTAGATTTCATGAGAGTGGGCTTTTCAATAATAATCACGTGATCTTCAGATCAAGTTCAGTGTGGGACAAAGATTTTTCACATCTATTTGATGTAAAGGATGCAATTACAAGAAATAATATTGCACCTATTAGGTCTAGCCAAGTAAGTCAATTACTACCCGAATCAAAAAGCTTATCTCAAGGGGATGATAATGGTGCAAAACATTTTCCATGGAGCACCTTTTCTAGTTCTATCAATTATCAACACAGCTCTTGCAGTCCTATCTTCACTGAGTACACAATATTTGGTTCTGAGGTATGTCCTTAA
Protein sequence

>MS.gene073152.t1

MGEIPSKDVKSESSSGIGEKPASWQIGRKRSFFDLNEDAVDDGDDHVILISEGPCGEISNNEISSQEGNLRSNNNSSEEGKGRGSTVRQYVRSKMPRLRWTPDLHLSFVHAVERLGGQERATPKLVLQLMNVRGLSIAHVKSHLQMYRSKKLDESGQVLSHKNRTQGTHHIMDMYGRFNVQGHFGVENNYLHPSSPLLKQPSYNEIKAHDTSRFHESGLFNNNHVIFRSSSVWDKDFSHLFDVKDAITRNNIAPIRSSQVSQLLPESKSLSQGDDNGAKHFPWSTFSSSINYQHSSCSPIFTEYTIFGSEVCP