AlfalfaGEDB | MS.gene07332

M. sativa cultivar XinJiangDaYe / MS.gene07332

KEGG: K09422
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF00249.34

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07332.t1	AHY94990.1	95.7	278	0	1	1	266	1	278	1.90E-151	545

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07332.t1	Q9LDE1	73.2	149	40	0	19	167	5	153	1.1e-64	248.1

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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07332.t1	A0A024A419	95.7	278	0	1	1	266	1	278	1.4e-151	545.0

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene07332.t1	TF	MYB

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI:

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07332.t1	MTR_1g086530	91.367	278	12	1	1	266	1	278	0.0	520
MS.gene07332.t1	MTR_1g086510	56.275	247	96	4	26	264	14	256	4.39e-89	265
MS.gene07332.t1	MTR_4g123040	73.759	141	37	0	26	166	17	157	1.26e-70	220
MS.gene07332.t1	MTR_2g033170	70.748	147	43	0	20	166	11	157	5.92e-70	219
MS.gene07332.t1	MTR_1g110460	60.959	146	57	0	33	178	26	171	2.97e-67	211
MS.gene07332.t1	MTR_7g035075	68.966	116	36	0	33	148	14	129	1.45e-57	183
MS.gene07332.t1	MTR_6g006030	72.222	108	30	0	33	140	14	121	4.62e-57	182
MS.gene07332.t1	MTR_1g045610	69.159	107	33	0	32	138	20	126	1.71e-51	169
MS.gene07332.t1	MTR_1g073170	65.714	105	36	0	34	138	21	125	3.97e-49	163
MS.gene07332.t1	MTR_4g063100	58.974	117	45	1	25	141	2	115	3.74e-46	155

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07332.t1	AT2G47190	48.846	260	107	5	26	265	13	266	1.72e-78	238
MS.gene07332.t1	AT3G06490	73.649	148	39	0	19	166	5	152	1.53e-74	230
MS.gene07332.t1	AT1G48000	67.347	147	47	1	33	178	32	178	5.01e-73	224
MS.gene07332.t1	AT5G49620	74.468	141	36	0	28	168	21	161	1.61e-71	222
MS.gene07332.t1	AT1G68320	66.901	142	47	0	33	174	19	160	6.61e-70	217
MS.gene07332.t1	AT5G49620	67.742	155	36	1	28	168	21	175	8.04e-68	213
MS.gene07332.t1	AT1G25340	54.301	186	66	3	34	219	19	185	4.64e-66	207
MS.gene07332.t1	AT1G25340	52.151	186	65	4	34	219	19	180	1.27e-60	193
MS.gene07332.t1	AT1G25340	52.151	186	65	4	34	219	19	180	3.62e-60	193
MS.gene07332.t1	AT3G27810	72.897	107	29	0	33	139	20	126	1.38e-57	184

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Find 72 sgRNAs with CRISPR-Local

Find 84 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATAGCGGAACAATTGCC+TGG	0.485517	1.4:+69031057	MS.gene07332:CDS
AAACATCCAAGAATTTGAAC+AGG	0.422533	1.4:+69031422	MS.gene07332:CDS
AAACCAATATTCATGTAATG+AGG	0.554808	1.4:+69030441	MS.gene07332:CDS
AACATCCAAGAATTTGAACA+GGG	0.633519	1.4:+69031423	MS.gene07332:CDS
AACTCCGTCGCTCGCTGTTC+GGG	0.308012	1.4:+69030599	MS.gene07332:CDS
AAGAATTTGAACAGGGGAAT+GGG	0.400055	1.4:+69031430	MS.gene07332:CDS
AAGTTTGGATTCTCTCAATG+AGG	0.626202	1.4:-69031203	None:intergenic
ACAAGTTTCACTATGGCTAT+AGG	0.431216	1.4:-69031250	None:intergenic
ACAATGAAATAAAGAACTAT+TGG	0.404212	1.4:+69031088	MS.gene07332:CDS
ACACGTACCGATTGCCCCAA+CGG	0.518769	1.4:-69030736	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAACATCCAAGAATTTGAAC+AGG	+	chr1.4:69031422-69031441	MS.gene07332:CDS	30.0%
AACATCCAAGAATTTGAACA+GGG	+	chr1.4:69031423-69031442	MS.gene07332:CDS	30.0%
ACTCCTCATTACATGAATAT+TGG	-	chr1.4:69030447-69030466	None:intergenic	30.0%
ATATTTCTTGCAGCAACAAT+TGG	+	chr1.4:69031488-69031507	MS.gene07332:CDS	30.0%
ATGTAAACAGTCAACAGTTT+AGG	+	chr1.4:69031151-69031170	MS.gene07332:CDS	30.0%
GATACTATGGTGATAAAGAA+AGG	+	chr1.4:69030509-69030528	MS.gene07332:CDS	30.0%
TACTATTCTGATGAACTATG+TGG	+	chr1.4:69030553-69030572	MS.gene07332:CDS	30.0%
TGTAAACAGTCAACAGTTTA+GGG	+	chr1.4:69031152-69031171	MS.gene07332:CDS	30.0%
TGTAAGTCAAGAAGATACTA+TGG	+	chr1.4:69030496-69030515	MS.gene07332:CDS	30.0%
AAACAAGGTTTGTGTGAAAG+AGG	-	chr1.4:69030946-69030965	None:intergenic	35.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	69030425	69031524	69030425	ID=MS.gene07332
chr1.4	mRNA	69030425	69031524	69030425	ID=MS.gene07332.t1;Parent=MS.gene07332
chr1.4	exon	69030425	69030750	69030425	ID=MS.gene07332.t1.exon1;Parent=MS.gene07332.t1
chr1.4	CDS	69030425	69030750	69030425	ID=cds.MS.gene07332.t1;Parent=MS.gene07332.t1
chr1.4	exon	69031050	69031524	69031050	ID=MS.gene07332.t1.exon2;Parent=MS.gene07332.t1
chr1.4	CDS	69031050	69031524	69031050	ID=cds.MS.gene07332.t1;Parent=MS.gene07332.t1

Gene Sequence

>MS.gene07332

ATGGATACCAATTACAAAACCAATATTCATGTAATGAGGAGTTTTTCAGATTGTGGTTCATCAGTAACGGATGTAAGTCAAGAAGATACTATGGTGATAAAGAAAGGTCCATGGACAGAAGAGGAGGATACTATTCTGATGAACTATGTGGCAGTCCACGGAGATGGTCACTGGAACTCCGTCGCTCGCTGTTCGGGTCTAAAGAGAACCGGGAAAAGCTGCAGATTAAGATGGTTGAACTACTTACGTCCAAATGTTCGTCGAGGGAACATCACTCTCCAAGAACAAGTCTTGATTCTTGATCTTCATTCCCGTTGGGGCAATCGGTACGTGTATATATATGCATGTAAACTATGTAATTGGTTAATTTATATATTAAGGTTGCATTATGATCGTGCACTACAAGAAATAAGACATTAAGCAACGATTAATTTGTTAGCAACATGCATATAGCTCATTTAACTCGTTGTAATAAATTGTTTAATTTCTTTCTATTGTAATATTATAAACATTTTTTTCCTCTTTCACACAAACCTTGTTTTCTCTAAAAAAAAACCTTGTTTAGTTTTAGAAGGAAATAGAGTTGGTCAAATTAACTAACATGATATTCTGTTATTTAATGAAGGTGGTCTAAAATAGCGGAACAATTGCCTGGAAGAACGGACAATGAAATAAAGAACTATTGGAGAACAAGAGTGGTGAAGCAAGCTAGGCAACTAAAATGTGATGTAAACAGTCAACAGTTTAGGGACACATTGCGTTTAGTATGGATTCCACGCCTCATTGAGAGAATCCAAACTTCTCGTGCAACTGTAGATACTAATTCCTATAGCCATAGTGAAACTTGTGGGGCAAATGCAAATCCTCCTATGTTGCAGTCTGAGGTTTCATCGTACTCGTCAGGGGTCGATGTTCAAGTACAATCGAACGTATCTTACAATTTAATGGATTGTGCGGAACTGGGATCCACGTGGCAGGAGTGGAACTGCTTAGACTCAAACATCCAAGAATTTGAACAGGGGAATGGGTTTGGTGGTTCAGAATTGTGGACAGATGAAAACATATATTTCTTGCAGCAACAATTGGCTGATGATTTATGA

Protein sequence

>MS.gene07332.t1

MDTNYKTNIHVMRSFSDCGSSVTDVSQEDTMVIKKGPWTEEEDTILMNYVAVHGDGHWNSVARCSGLKRTGKSCRLRWLNYLRPNVRRGNITLQEQVLILDLHSRWGNRWSKIAEQLPGRTDNEIKNYWRTRVVKQARQLKCDVNSQQFRDTLRLVWIPRLIERIQTSRATVDTNSYSHSETCGANANPPMLQSEVSSYSSGVDVQVQSNVSYNLMDCAELGSTWQEWNCLDSNIQEFEQGNGFGGSELWTDENIYFLQQQLADDL