AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073376

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073376.t1 MTR_1g057920 97.087 103 3 0 115 217 1 103 3.21e-71 213
MS.gene073376.t1 MTR_7g113010 46.914 243 61 7 29 220 290 515 1.01e-52 179
MS.gene073376.t1 MTR_6g017200 65.546 119 38 1 102 217 9 127 1.14e-48 157
MS.gene073376.t1 MTR_0402s0040 73.684 95 25 0 123 217 1 95 4.34e-42 149
MS.gene073376.t1 MTR_2g070590 72.619 84 23 0 134 217 3 86 2.62e-37 127
MS.gene073376.t1 MTR_0137s0040 58.654 104 41 1 115 216 1 104 1.00e-34 120
MS.gene073376.t1 MTR_0291s0060 70.270 74 22 0 125 198 12 85 7.19e-30 107
MS.gene073376.t1 MTR_5g015480 55.340 103 42 1 115 217 27 125 5.63e-28 107
MS.gene073376.t1 MTR_1g091013 48.052 77 29 1 140 216 4 69 8.79e-15 67.0
MS.gene073376.t1 MTR_4g102720 41.429 70 40 1 148 217 7 75 8.92e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 57 sgRNAs with CRISPR-Local

Find 170 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGTGATGAACATTTCGTTCA+TGG 0.222929 2.4:+54584117 None:intergenic
TTCCAGATTGTGTCGATAAA+TGG 0.224458 2.4:-54584151 MS.gene073376:CDS
TTTGGTATTCGTCGAGAATA+TGG 0.287007 2.4:-54583993 MS.gene073376:CDS
CCATGCATCGTCCCCTCTTC+TGG 0.307554 2.4:-54584686 MS.gene073376:CDS
AATGATTACAGTAAAAGAAT+TGG 0.319753 2.4:-54584017 MS.gene073376:CDS
TACAGTAAAAGAATTGGATT+TGG 0.322823 2.4:-54584011 MS.gene073376:CDS
ACCCATTTATCGACACAATC+TGG 0.348105 2.4:+54584149 None:intergenic
TTCATTCATCTCTCAATAAC+AGG 0.354642 2.4:-54584724 MS.gene073376:CDS
CTTCGCTTCACAGTTCTTTG+AGG 0.374046 2.4:-54585323 MS.gene073376:CDS
GGAAATAAAAGCAGAATAGA+TGG 0.381196 2.4:-54583972 MS.gene073376:CDS
GAGCAGAAAGACAGAATAGA+TGG 0.403020 2.4:-54583866 MS.gene073376:CDS
TGCCAATAGTGGACCTCACT+TGG 0.426768 2.4:-54584081 MS.gene073376:CDS
TCCCAAGTGAGGTCCACTAT+TGG 0.441740 2.4:+54584079 None:intergenic
TCCAGATTGTGTCGATAAAT+GGG 0.451957 2.4:-54584150 MS.gene073376:CDS
CGATGCATGGAGTGGGGCAG+AGG 0.452027 2.4:+54584699 None:intergenic
GAGGGGACGATGCATGGAGT+GGG 0.461143 2.4:+54584692 None:intergenic
AAAGAAAGTATTGCCAATAG+TGG 0.465982 2.4:-54584092 MS.gene073376:CDS
AATGGATGAGGTTGTTGAAA+TGG 0.467837 2.4:-54586538 MS.gene073376:CDS
GAGATGAACGGCGGAACAAT+CGG 0.484862 2.4:+54584758 None:intergenic
CCGCCGTTCATCTCAATCGC+TGG 0.487567 2.4:-54584749 MS.gene073376:CDS
TCTGATACCATGATTGCATA+AGG 0.500320 2.4:+54585288 None:intergenic
CCAGAAGAGGGGACGATGCA+TGG 0.510778 2.4:+54584686 None:intergenic
CAACATAATTACACAGCTGA+GGG 0.525854 2.4:+54586495 None:intergenic
GCAGTTTGAATCTGAGGCGG+TGG 0.555318 2.4:-54584054 MS.gene073376:CDS
GGGAAACGAAGAGGTTCTGA+GGG 0.565476 2.4:+54584802 None:intergenic
AGAGGGGACGATGCATGGAG+TGG 0.567367 2.4:+54584691 None:intergenic
TCCGCCAGTGTCATCTCTGA+AGG 0.567435 2.4:-54585349 MS.gene073376:CDS
GGGAATGCAGTTTGAATCTG+AGG 0.567573 2.4:-54584060 MS.gene073376:CDS
GCCAATAGTGGACCTCACTT+GGG 0.571514 2.4:-54584080 MS.gene073376:CDS
AGGGAAACGAAGAGGTTCTG+AGG 0.574050 2.4:+54584801 None:intergenic
CGGTTGTTGACGCTGATGAG+AGG 0.578012 2.4:+54584781 None:intergenic
AGACAAAGGTTACCAGAAGA+GGG 0.579967 2.4:+54584674 None:intergenic
AAACGACGGCGACGAGACAA+AGG 0.585953 2.4:+54584660 None:intergenic
TCAACATAATTACACAGCTG+AGG 0.591713 2.4:+54586494 None:intergenic
GGTTGTTGACGCTGATGAGA+GGG 0.595302 2.4:+54584782 None:intergenic
GAGACAAAGGTTACCAGAAG+AGG 0.598921 2.4:+54584673 None:intergenic
GGATGAGGTTGTTGAAATGG+TGG 0.599899 2.4:-54586535 MS.gene073376:CDS
AGGGGACGATGCATGGAGTG+GGG 0.610284 2.4:+54584693 None:intergenic
TCTGCTCTAGACTCCGCCGT+CGG 0.614843 2.4:+54583882 None:intergenic
ACGTACTTTCTCAGAACACA+TGG 0.616421 2.4:-54584186 MS.gene073376:intron
TCAGAGATGACACTGGCGGA+AGG 0.623318 2.4:+54585352 None:intergenic
GATTGTTTGATTTGAAACGA+CGG 0.641951 2.4:+54584646 None:intergenic
AAGATTCACATGCTTCAAAG+AGG 0.641966 2.4:-54583934 MS.gene073376:CDS
AGATTCACATGCTTCAAAGA+GGG 0.653445 2.4:-54583933 MS.gene073376:CDS
GGTTGTTGAAATGGTGGACG+AGG 0.655225 2.4:-54586529 MS.gene073376:CDS
TCTGAATTGAGAAATGGATG+AGG 0.656124 2.4:-54586550 None:intergenic
TCAAACTGCATTCCCAAGTG+AGG 0.657944 2.4:+54584068 None:intergenic
CTGATGAGAGGGAAACGAAG+AGG 0.664098 2.4:+54584793 None:intergenic
TGTCAAAAGACGTCAACCGA+CGG 0.667892 2.4:-54583898 MS.gene073376:CDS
ACACCAGCGATTGAGATGAA+CGG 0.683609 2.4:+54584746 None:intergenic
GACAAAGGTTACCAGAAGAG+GGG 0.686375 2.4:+54584675 None:intergenic
TCCTTCAGAGATGACACTGG+CGG 0.686635 2.4:+54585348 None:intergenic
ATGAACGGCGGAACAATCGG+CGG 0.695148 2.4:+54584761 None:intergenic
GAGTCCTTCAGAGATGACAC+TGG 0.698617 2.4:+54585345 None:intergenic
CAAAAGACGTCAACCGACGG+CGG 0.716712 2.4:-54583895 MS.gene073376:CDS
AATGCAGTTTGAATCTGAGG+CGG 0.731388 2.4:-54584057 MS.gene073376:CDS
CCAGCGATTGAGATGAACGG+CGG 0.741374 2.4:+54584749 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAGAAAAGAATAAACATAA+AGG + chr2.4:54586154-54586173 None:intergenic 15.0%
!!! ATAATTTTCTGAAAATATGT+AGG + chr2.4:54584565-54584584 None:intergenic 15.0%
!!! TACATATTTTCAGAAAATTA+TGG - chr2.4:54584564-54584583 MS.gene073376:intron 15.0%
!! AATGATTACAGTAAAAGAAT+TGG - chr2.4:54586377-54586396 MS.gene073376:intron 20.0%
!! CATATTTATATATCTGAATG+AGG - chr2.4:54584929-54584948 MS.gene073376:intron 20.0%
!! TAAACAAATTGTGAATAACT+AGG + chr2.4:54584720-54584739 None:intergenic 20.0%
!! TCAACTAATAAGTTCAAAAT+GGG + chr2.4:54584809-54584828 None:intergenic 20.0%
!! TGGTATTAAAAAGAAAGAAA+AGG + chr2.4:54584463-54584482 None:intergenic 20.0%
!!! CATTTTTAGCAATAAAACAA+TGG + chr2.4:54584672-54584691 None:intergenic 20.0%
!!! TATGTTCTTTGTTATTGTTA+AGG - chr2.4:54585327-54585346 MS.gene073376:CDS 20.0%
! AGACTAATTTATGACACTAA+AGG + chr2.4:54584236-54584255 None:intergenic 25.0%
! GAAATGTTGAAAACAACAAA+CGG + chr2.4:54586132-54586151 None:intergenic 25.0%
! GTCAACTAATAAGTTCAAAA+TGG + chr2.4:54584810-54584829 None:intergenic 25.0%
! TACAGTAAAAGAATTGGATT+TGG - chr2.4:54586383-54586402 MS.gene073376:intron 25.0%
! TAGCAATAAAACAATGGAAA+AGG + chr2.4:54584666-54584685 None:intergenic 25.0%
! TATAATGTCTGAAAAATCAG+AGG - chr2.4:54584385-54584404 MS.gene073376:intron 25.0%
!! AGTGTCATAAATTAGTCTAT+AGG - chr2.4:54584238-54584257 MS.gene073376:intron 25.0%
!! GTGGTATGTGAATTTTATAT+CGG - chr2.4:54585787-54585806 MS.gene073376:intron 25.0%
!!! CTATTGATTTGTGCATTATT+TGG - chr2.4:54585812-54585831 MS.gene073376:intron 25.0%
!!! GTTTTTTTGAACTTTGGATT+TGG - chr2.4:54584338-54584357 MS.gene073376:intron 25.0%
!!! TATATGGTCTAAGCTTTATT+TGG - chr2.4:54585846-54585865 MS.gene073376:intron 25.0%
!!! TATTTGGTTTGTACATTTTC+TGG - chr2.4:54586001-54586020 MS.gene073376:intron 25.0%
!!! TTTGGTTTGTGAATCATATA+TGG - chr2.4:54585830-54585849 MS.gene073376:intron 25.0%
AGCACATGAATAAGAATAGT+AGG + chr2.4:54586061-54586080 None:intergenic 30.0%
ATAGGTGTTTGATGAAATTC+TGG - chr2.4:54583947-54583966 MS.gene073376:CDS 30.0%
ATGAATACAGCAAGAAACTT+TGG + chr2.4:54584762-54584781 None:intergenic 30.0%
CGGATAAAAGATGATCTATA+TGG - chr2.4:54585360-54585379 MS.gene073376:intron 30.0%
CTAAAATACACTTGTAGAGT+TGG + chr2.4:54584426-54584445 None:intergenic 30.0%
CTGTATTCATGAATCTTGAA+TGG - chr2.4:54584772-54584791 MS.gene073376:CDS 30.0%
GGAAATAAAAGCAGAATAGA+TGG - chr2.4:54586422-54586441 MS.gene073376:intron 30.0%
TAGTTGCAAATTGTCTTGAT+TGG - chr2.4:54584210-54584229 MS.gene073376:intron 30.0%
TCATGAATCTTGAATGGAAT+TGG - chr2.4:54584778-54584797 MS.gene073376:CDS 30.0%
TTCATTCATCTCTCAATAAC+AGG - chr2.4:54585670-54585689 MS.gene073376:intron 30.0%
! CGTAGATTTTCATTAACGTA+AGG - chr2.4:54584596-54584615 MS.gene073376:intron 30.0%
!! AAAGAAAGTATTGCCAATAG+TGG - chr2.4:54586302-54586321 MS.gene073376:intron 30.0%
!! ATCAAGGAAAGTATTGTGTA+AGG + chr2.4:54584034-54584053 None:intergenic 30.0%
!! GATTGTTTGATTTGAAACGA+CGG + chr2.4:54585751-54585770 None:intergenic 30.0%
!! TCAAGGAAAGTATTGTGTAA+GGG + chr2.4:54584033-54584052 None:intergenic 30.0%
!!! GTGGAAGTTTTTTTGAACTT+TGG - chr2.4:54584332-54584351 MS.gene073376:intron 30.0%
!!! GTTTGATGAAATTCTGGTTT+TGG - chr2.4:54583953-54583972 MS.gene073376:CDS 30.0%
!!! TTCTAAGATGCTTCCAATTT+TGG - chr2.4:54584293-54584312 MS.gene073376:intron 30.0%
AAGATTCACATGCTTCAAAG+AGG - chr2.4:54586460-54586479 MS.gene073376:intron 35.0%
AATGTCTGAAAAATCAGAGG+TGG - chr2.4:54584388-54584407 MS.gene073376:intron 35.0%
ACAAATGTTGAGAAATGGTC+AGG + chr2.4:54585009-54585028 None:intergenic 35.0%
AGAACCCAAATTCTTAACTG+TGG - chr2.4:54584059-54584078 MS.gene073376:CDS 35.0%
AGATTCACATGCTTCAAAGA+GGG - chr2.4:54586461-54586480 MS.gene073376:intron 35.0%
AGTTCAAAAAAACTTCCACC+AGG + chr2.4:54584331-54584350 None:intergenic 35.0%
CAAATGCAGTAACTAATGTG+TGG + chr2.4:54584489-54584508 None:intergenic 35.0%
CAACATAATTACACAGCTGA+GGG + chr2.4:54583902-54583921 None:intergenic 35.0%
CACACATTAGTTACTGCATT+TGG - chr2.4:54584487-54584506 MS.gene073376:intron 35.0%
CATTGTTTACTGTGACGAAT+AGG - chr2.4:54583929-54583948 MS.gene073376:CDS 35.0%
CCAACACAAATGTTGAGAAA+TGG + chr2.4:54585014-54585033 None:intergenic 35.0%
CCATTTCTCAACATTTGTGT+TGG - chr2.4:54585011-54585030 MS.gene073376:intron 35.0%
CTACTGCTTTAATATGCTCA+TGG - chr2.4:54585131-54585150 MS.gene073376:intron 35.0%
GAACCCAAATTCTTAACTGT+GGG - chr2.4:54584060-54584079 MS.gene073376:CDS 35.0%
GTTCAAAAAAACTTCCACCA+GGG + chr2.4:54584330-54584349 None:intergenic 35.0%
TACAGCAAGAAACTTTGGTT+TGG + chr2.4:54584757-54584776 None:intergenic 35.0%
TAGGAACTAAGTATACCTTG+CGG + chr2.4:54584701-54584720 None:intergenic 35.0%
TATCCATTCTACATCACATG+CGG - chr2.4:54583988-54584007 MS.gene073376:CDS 35.0%
TCAACATAATTACACAGCTG+AGG + chr2.4:54583903-54583922 None:intergenic 35.0%
TCTGATACCATGATTGCATA+AGG + chr2.4:54585109-54585128 None:intergenic 35.0%
TGAATCTTGAATGGAATTGG+CGG - chr2.4:54584781-54584800 MS.gene073376:CDS 35.0%
TGATGTGTTTATGAGACTCT+TGG - chr2.4:54584964-54584983 MS.gene073376:intron 35.0%
TTTGGTATTCGTCGAGAATA+TGG - chr2.4:54586401-54586420 MS.gene073376:intron 35.0%
! AGATCATCTTTTATCCGACA+TGG + chr2.4:54585357-54585376 None:intergenic 35.0%
! CATGAGTATTGTGCATTTAC+TGG - chr2.4:54585963-54585982 MS.gene073376:intron 35.0%
! CTTTTGACCTTATGCAATCA+TGG - chr2.4:54585099-54585118 MS.gene073376:intron 35.0%
! GATCATCTTTTATCCGACAT+GGG + chr2.4:54585356-54585375 None:intergenic 35.0%
! TCCAGATTGTGTCGATAAAT+GGG - chr2.4:54586244-54586263 MS.gene073376:intron 35.0%
! TTCCAGATTGTGTCGATAAA+TGG - chr2.4:54586243-54586262 MS.gene073376:intron 35.0%
!! ACTCGTCTTAGTATTGATAG+TGG - chr2.4:54585168-54585187 MS.gene073376:intron 35.0%
!! ATTGGAAGCATCTTAGAACT+TGG + chr2.4:54584291-54584310 None:intergenic 35.0%
!! GATGCTTCCAATTTTGGTAA+TGG - chr2.4:54584299-54584318 MS.gene073376:intron 35.0%
!!! ATCTCTGTCGTTTTAAACAG+TGG - chr2.4:54585768-54585787 MS.gene073376:intron 35.0%
!!! TTCCAATTTTGGTAATGGCA+TGG - chr2.4:54584304-54584323 MS.gene073376:intron 35.0%
AAGACCCTAAATGGGGTAAA+TGG + chr2.4:54585416-54585435 None:intergenic 40.0%
AATGCAGTTTGAATCTGAGG+CGG - chr2.4:54586337-54586356 MS.gene073376:intron 40.0%
ACCCATTTATCGACACAATC+TGG + chr2.4:54586248-54586267 None:intergenic 40.0%
ACGTACTTTCTCAGAACACA+TGG - chr2.4:54586208-54586227 MS.gene073376:intron 40.0%
ACTCATCCAAGACCCTAAAT+GGG + chr2.4:54585424-54585443 None:intergenic 40.0%
AGACAAAGGTTACCAGAAGA+GGG + chr2.4:54585723-54585742 None:intergenic 40.0%
AGCTCCATTTACCCCATTTA+GGG - chr2.4:54585409-54585428 MS.gene073376:intron 40.0%
AGTTGACACTTGATGCATGT+GGG - chr2.4:54584823-54584842 MS.gene073376:intron 40.0%
ATTGTTAAGGAGTCCCATGT+CGG - chr2.4:54585340-54585359 MS.gene073376:CDS 40.0%
CAAATTCTTAACTGTGGGCT+GGG - chr2.4:54584065-54584084 MS.gene073376:CDS 40.0%
CAGCCCACAGTTAAGAATTT+GGG + chr2.4:54584066-54584085 None:intergenic 40.0%
CAGTAACTAATGTGTGGCAT+TGG + chr2.4:54584483-54584502 None:intergenic 40.0%
CGTGATGAACATTTCGTTCA+TGG + chr2.4:54586280-54586299 None:intergenic 40.0%
CTCAACATTTGTGTTGGACA+GGG - chr2.4:54585017-54585036 MS.gene073376:intron 40.0%
GACACATGAGTTCCTTAAGT+TGG - chr2.4:54585194-54585213 MS.gene073376:intron 40.0%
GAGCAGAAAGACAGAATAGA+TGG - chr2.4:54586528-54586547 MS.gene073376:CDS 40.0%
GTGATGATATGTGGCTGAAA+TGG - chr2.4:54585226-54585245 MS.gene073376:intron 40.0%
TAAATGGAGCTGGTGGTAAA+TGG + chr2.4:54585400-54585419 None:intergenic 40.0%
TAACCGCATGTGATGTAGAA+TGG + chr2.4:54583994-54584013 None:intergenic 40.0%
TAGTTGACACTTGATGCATG+TGG - chr2.4:54584822-54584841 MS.gene073376:intron 40.0%
TCAACATTTGTGTTGGACAG+GGG - chr2.4:54585018-54585037 MS.gene073376:intron 40.0%
TCTCAACATTTGTGTTGGAC+AGG - chr2.4:54585016-54585035 MS.gene073376:intron 40.0%
TTCTACATCACATGCGGTTA+TGG - chr2.4:54583994-54584013 MS.gene073376:CDS 40.0%
TTGGTGGAAGTGATGATATG+TGG - chr2.4:54585217-54585236 MS.gene073376:intron 40.0%
! ATTTACCCCATTTAGGGTCT+TGG - chr2.4:54585415-54585434 MS.gene073376:intron 40.0%
! ATTTGGGTTCTGTGCAATCA+AGG + chr2.4:54584050-54584069 None:intergenic 40.0%
! CGTGGCAAAAATGGATTCAA+TGG - chr2.4:54585448-54585467 MS.gene073376:intron 40.0%
! GTTTTTGCATCGCTGCTATT+TGG - chr2.4:54585985-54586004 MS.gene073376:intron 40.0%
!! CATGAGTTCCTTAAGTTGGT+TGG - chr2.4:54585198-54585217 MS.gene073376:intron 40.0%
ACACCAGCGATTGAGATGAA+CGG + chr2.4:54585651-54585670 None:intergenic 45.0%
AGATGATCTATATGGAGCGC+AGG - chr2.4:54585368-54585387 MS.gene073376:intron 45.0%
ATAGCCATACTAGTGTGTCG+TGG + chr2.4:54585304-54585323 None:intergenic 45.0%
CACTCATCCAAGACCCTAAA+TGG + chr2.4:54585425-54585444 None:intergenic 45.0%
CACTTCCACCAACCAACTTA+AGG + chr2.4:54585209-54585228 None:intergenic 45.0%
CAGCTCCATTTACCCCATTT+AGG - chr2.4:54585408-54585427 MS.gene073376:intron 45.0%
CCAAATTCTTAACTGTGGGC+TGG - chr2.4:54584064-54584083 MS.gene073376:CDS 45.0%
CCAGCCCACAGTTAAGAATT+TGG + chr2.4:54584067-54584086 None:intergenic 45.0%
CTAAAAATGCTAGCTCCGCA+AGG - chr2.4:54584683-54584702 MS.gene073376:CDS 45.0%
CTAAATGGGGTAAATGGAGC+TGG + chr2.4:54585410-54585429 None:intergenic 45.0%
CTCATCCAAGACCCTAAATG+GGG + chr2.4:54585423-54585442 None:intergenic 45.0%
CTTCGCTTCACAGTTCTTTG+AGG - chr2.4:54585071-54585090 MS.gene073376:intron 45.0%
GACAAAGGTTACCAGAAGAG+GGG + chr2.4:54585722-54585741 None:intergenic 45.0%
GAGACAAAGGTTACCAGAAG+AGG + chr2.4:54585724-54585743 None:intergenic 45.0%
GGATGAGGTTGTTGAAATGG+TGG - chr2.4:54583859-54583878 MS.gene073376:CDS 45.0%
GGCCATGCCATTACCAAAAT+TGG + chr2.4:54584309-54584328 None:intergenic 45.0%
GGGAATGCAGTTTGAATCTG+AGG - chr2.4:54586334-54586353 MS.gene073376:intron 45.0%
TCAAACTGCATTCCCAAGTG+AGG + chr2.4:54586329-54586348 None:intergenic 45.0%
TGAGTGACACGTGGCAAAAA+TGG - chr2.4:54585439-54585458 MS.gene073376:intron 45.0%
TGGCTGAAATGGTGAAGAGA+AGG - chr2.4:54585237-54585256 MS.gene073376:intron 45.0%
TGTCAAAAGACGTCAACCGA+CGG - chr2.4:54586496-54586515 MS.gene073376:intron 45.0%
! GAGTGTTGCAGGAGATTTAC+AGG + chr2.4:54584642-54584661 None:intergenic 45.0%
!! ATGGAAAAGGAGAGTGTTGC+AGG + chr2.4:54584653-54584672 None:intergenic 45.0%
!! GAGTTCCTTAAGTTGGTTGG+TGG - chr2.4:54585201-54585220 MS.gene073376:intron 45.0%
!! TAATTAAAAAAAAACATTAA+AGG - chr2.4:54585504-54585523 MS.gene073376:intron 5.0%
AATGGGGTAAATGGAGCTGG+TGG + chr2.4:54585407-54585426 None:intergenic 50.0%
ACACTTGATGCATGTGGGCT+AGG - chr2.4:54584828-54584847 MS.gene073376:intron 50.0%
AGGGAAACGAAGAGGTTCTG+AGG + chr2.4:54585596-54585615 None:intergenic 50.0%
CTGATGAGAGGGAAACGAAG+AGG + chr2.4:54585604-54585623 None:intergenic 50.0%
GACACCACGACACACTAGTA+TGG - chr2.4:54585297-54585316 MS.gene073376:CDS 50.0%
GAGATGAACGGCGGAACAAT+CGG + chr2.4:54585639-54585658 None:intergenic 50.0%
GAGTCCTTCAGAGATGACAC+TGG + chr2.4:54585052-54585071 None:intergenic 50.0%
GGTGTCCAAAATCGACGAGT+CGG + chr2.4:54585283-54585302 None:intergenic 50.0%
GGTTGTTGACGCTGATGAGA+GGG + chr2.4:54585615-54585634 None:intergenic 50.0%
GTGTCCAAAATCGACGAGTC+GGG + chr2.4:54585282-54585301 None:intergenic 50.0%
TCCCAAGTGAGGTCCACTAT+TGG + chr2.4:54586318-54586337 None:intergenic 50.0%
TCCTTCAGAGATGACACTGG+CGG + chr2.4:54585049-54585068 None:intergenic 50.0%
TGTCCAAAATCGACGAGTCG+GGG + chr2.4:54585281-54585300 None:intergenic 50.0%
! GGGAAACGAAGAGGTTCTGA+GGG + chr2.4:54585595-54585614 None:intergenic 50.0%
!! GCCAATAGTGGACCTCACTT+GGG - chr2.4:54586314-54586333 MS.gene073376:intron 50.0%
!! GGTTGTTGAAATGGTGGACG+AGG - chr2.4:54583865-54583884 MS.gene073376:CDS 50.0%
!! TGCCAATAGTGGACCTCACT+TGG - chr2.4:54586313-54586332 MS.gene073376:intron 50.0%
!!! TTTTGGTAATGGCATGGCCC+TGG - chr2.4:54584310-54584329 MS.gene073376:intron 50.0%
AAACGACGGCGACGAGACAA+AGG + chr2.4:54585737-54585756 None:intergenic 55.0%
ATGAACGGCGGAACAATCGG+CGG + chr2.4:54585636-54585655 None:intergenic 55.0%
CAAAAGACGTCAACCGACGG+CGG - chr2.4:54586499-54586518 MS.gene073376:intron 55.0%
CCAGCGATTGAGATGAACGG+CGG + chr2.4:54585648-54585667 None:intergenic 55.0%
CGGTTGTTGACGCTGATGAG+AGG + chr2.4:54585616-54585635 None:intergenic 55.0%
GGTCTTGGATGAGTGACACG+TGG - chr2.4:54585430-54585449 MS.gene073376:intron 55.0%
GTCCAAAATCGACGAGTCGG+GGG + chr2.4:54585280-54585299 None:intergenic 55.0%
TCAGAGATGACACTGGCGGA+AGG + chr2.4:54585045-54585064 None:intergenic 55.0%
TCCAAAATCGACGAGTCGGG+GGG + chr2.4:54585279-54585298 None:intergenic 55.0%
TCCGCCAGTGTCATCTCTGA+AGG - chr2.4:54585045-54585064 MS.gene073376:intron 55.0%
! GCAGTTTGAATCTGAGGCGG+TGG - chr2.4:54586340-54586359 MS.gene073376:intron 55.0%
AGAGGGGACGATGCATGGAG+TGG + chr2.4:54585706-54585725 None:intergenic 60.0%
AGGGGACGATGCATGGAGTG+GGG + chr2.4:54585704-54585723 None:intergenic 60.0%
CAAAATCGACGAGTCGGGGG+GGG + chr2.4:54585277-54585296 None:intergenic 60.0%
CCAAAATCGACGAGTCGGGG+GGG + chr2.4:54585278-54585297 None:intergenic 60.0%
CCAGAAGAGGGGACGATGCA+TGG + chr2.4:54585711-54585730 None:intergenic 60.0%
CCATGCATCGTCCCCTCTTC+TGG - chr2.4:54585708-54585727 MS.gene073376:intron 60.0%
CCGCCGTTCATCTCAATCGC+TGG - chr2.4:54585645-54585664 MS.gene073376:intron 60.0%
GAGGGGACGATGCATGGAGT+GGG + chr2.4:54585705-54585724 None:intergenic 60.0%
! TCTGCTCTAGACTCCGCCGT+CGG + chr2.4:54586515-54586534 None:intergenic 60.0%
!! TGGTAATGGCATGGCCCTGG+TGG - chr2.4:54584313-54584332 MS.gene073376:intron 60.0%
CGATGCATGGAGTGGGGCAG+AGG + chr2.4:54585698-54585717 None:intergenic 65.0%
!!! CCCCCCCGACTCGTCGATTT+TGG - chr2.4:54585275-54585294 MS.gene073376:intron 65.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 54583857 54586559 54583857 ID=MS.gene073376
chr2.4 mRNA 54583857 54586559 54583857 ID=MS.gene073376.t1;Parent=MS.gene073376
chr2.4 exon 54586507 54586559 54586507 ID=MS.gene073376.t1.exon1;Parent=MS.gene073376.t1
chr2.4 CDS 54586507 54586559 54586507 ID=cds.MS.gene073376.t1;Parent=MS.gene073376.t1
chr2.4 exon 54585296 54585378 54585296 ID=MS.gene073376.t1.exon2;Parent=MS.gene073376.t1
chr2.4 CDS 54585296 54585378 54585296 ID=cds.MS.gene073376.t1;Parent=MS.gene073376.t1
chr2.4 exon 54584627 54584827 54584627 ID=MS.gene073376.t1.exon3;Parent=MS.gene073376.t1
chr2.4 CDS 54584627 54584827 54584627 ID=cds.MS.gene073376.t1;Parent=MS.gene073376.t1
chr2.4 exon 54583857 54584194 54583857 ID=MS.gene073376.t1.exon4;Parent=MS.gene073376.t1
chr2.4 CDS 54583857 54584194 54583857 ID=cds.MS.gene073376.t1;Parent=MS.gene073376.t1
Gene Sequence

>MS.gene073376

ATGGATGAGGTTGTTGAAATGGTGGACGAGGCTTCTGATAAACCCTCAGCTGTGTAATTATGTTGAGAATTTCATTGTTTACTGTGACGAATAGGTGTTTGATGAAATTCTGGTTTTGGTTGCTCTTTAATTATCCATTCTACATCACATGCGGTTATGGTTCTTCTGATGAGCCCTTACACAATACTTTCCTTGATTGCACAGAACCCAAATTCTTAACTGTGGGCTGGGTGTTAATTAAGTGTGTGTTTAACAAATGTGTTGCTGAATTAACGTAGATTATTTTTTCTGTTTTGCAGTTGCAAAAATAAAATTAATTTAGATTTTGTTATTAATTTGATTAAAATTGACTGTAGTTGCAAATTGTCTTGATTGGCCTTTAGTGTCATAAATTAGTCTATAGGTTTTTGAGAGTTTTGTGTGTGTTTTTGCCAAGTTCTAAGATGCTTCCAATTTTGGTAATGGCATGGCCCTGGTGGAAGTTTTTTTGAACTTTGGATTTGGTTTAATTTTCATAAGAATGAAAATTATAATGTCTGAAAAATCAGAGGTGGTGACTAGTTGAACCAACTCTACAAGTGTATTTTAGCGAGTTTTGTTTTGCCTTTTCTTTCTTTTTAATACCAATGCCACACATTAGTTACTGCATTTGGTACTAGTTTGTTTTCTTGATATATATCTTTGAACATGTACAACATTTTCTATCCTACATATTTTCAGAAAATTATGGTATCTTTTTCGTAGATTTTCATTAACGTAAGGAAATGATGTTATTATTTGATCCTGTAAATCTCCTGCAACACTCTCCTTTTCCATTGTTTTATTGCTAAAAATGCTAGCTCCGCAAGGTATACTTAGTTCCTAGTTATTCACAATTTGTTTATTTGATTCATGATACCAAACCAAAGTTTCTTGCTGTATTCATGAATCTTGAATGGAATTGGCGGTTCCCATTTTGAACTTATTAGTTGACACTTGATGCATGTGGGCTAGGATACTGTCTTCATGAATTGTAATTGTATAATGTTAGTGTACACACTGAACTCTGAGAACTTGTACAGAATTGCTACTGCATATTTATATATCTGAATGAGGTAAATCACATCGTGATGTGTTTATGAGACTCTTGGATTACTCTTCTGTTCATTACCTGACCATTTCTCAACATTTGTGTTGGACAGGGGACCTTCCGCCAGTGTCATCTCTGAAGGACTCTTCGCTTCACAGTTCTTTGAGGCAACACTTTTGACCTTATGCAATCATGGTATCAGATGCTACTGCTTTAATATGCTCATGGATAATTGATGCACAACTCGTCTTAGTATTGATAGTGGCATGACACATGAGTTCCTTAAGTTGGTTGGTGGAAGTGATGATATGTGGCTGAAATGGTGAAGAGAAGGATTGTAAAAGTTCACCCCCCCCGACTCGTCGATTTTGGACACCACGACACACTAGTATGGCTATAGTTATGTTCTTTGTTATTGTTAAGGAGTCCCATGTCGGATAAAAGATGATCTATATGGAGCGCAGGTATGCTCCATTTACCACCAGCTCCATTTACCCCATTTAGGGTCTTGGATGAGTGACACGTGGCAAAAATGGATTCAATGGTTCAGATTAAAATAAATAAAACATCAGTTTTTTTAATTAAAAAAAAACATTAAAGGAGAAACACGCGCTTCTCTTCTCTTCTTCTCTCATACGTGAACGTTGCTCACTCTCTTCTCAGCTTCCCTCAGAACCTCTTCGTTTCCCTCTCATCAGCGTCAACAACCGCCGATTGTTCCGCCGTTCATCTCAATCGCTGGTGTTCATTCATCTCTCAATAACAGGATCCTCTGCCCCACTCCATGCATCGTCCCCTCTTCTGGTAACCTTTGTCTCGTCGCCGTCGTTTCAAATCAAACAATCTCTGTCGTTTTAAACAGTGGTATGTGAATTTTATATCGGTGCTATTGATTTGTGCATTATTTGGTTTGTGAATCATATATGGTCTAAGCTTTATTTGGTTTGTGCATTGCTGCTATGAGTTTTATATCGCTGCTATTGATTTGTTCTGTTAAGACTACTGCTACTATTAGCTGCTATGTGATCTATTTCAAACATGAGTATTGTGCATTTACTGGTTTTTGCATCGCTGCTATTTGGTTTGTACATTTTCTGGTTTTTTAAACGCTGCTATTGCTAATTCTGCTATTCCTACTATTCTTATTCATGTGCTATCTAATTTATATGATGAAATTTCTTGTATGCACTTCTTTAATTTAAACCGTTTGTTGTTTTCAACATTTCCTTTATGTTTATTCTTTTCTTATTGCTCATCACAATCTCTGACACTAAGACTCTGTACGTACTTTCTCAGAACACATGGATTCATCATCAATTCCAGATTGTGTCGATAAATGGGTGAACAATATCCATGAACGAAATGTTCATCACGAGAAAGAAAGTATTGCCAATAGTGGACCTCACTTGGGAATGCAGTTTGAATCTGAGGCGGTGGCATATGAATTTTATAATGATTACAGTAAAAGAATTGGATTTGGTATTCGTCGAGAATATGGAAATAAAAGCAGAATAGATGGAGTTTTGACTTCGAGAAGATTCACATGCTTCAAAGAGGGTGCGCGAGTTGTTGTCAAAAGACGTCAACCGACGGCGGAGTCTAGAGCAGAAAGACAGAATAGATGGAGTTTTTAA
Protein sequence

>MS.gene073376.t1

MDEVVEMVDEASDKPSAVGPSASVISEGLFASQFFEATLLTLCNHASLRTSSFPSHQRQQPPIVPPFISIAGVHSSLNNRILCPTPCIVPSSGNLCLVAVVSNQTISVVLNSEHMDSSSIPDCVDKWVNNIHERNVHHEKESIANSGPHLGMQFESEAVAYEFYNDYSKRIGFGIRREYGNKSRIDGVLTSRRFTCFKEGARVVVKRRQPTAESRAERQNRWSF