AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073381

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073381.t1 MTR_2g437240 78.606 416 83 2 1 416 1 410 0.0 655
MS.gene073381.t1 MTR_2g437200 73.515 404 101 2 1 404 1 398 0.0 597
MS.gene073381.t1 MTR_2g438020 73.890 383 99 1 37 419 70 451 0.0 568
MS.gene073381.t1 MTR_2g437150 65.250 400 100 3 25 420 42 406 0.0 516
MS.gene073381.t1 MTR_2g437170 64.851 404 102 4 19 417 35 403 0.0 512
MS.gene073381.t1 MTR_2g437160 62.060 398 113 3 25 420 50 411 8.87e-173 489
MS.gene073381.t1 MTR_2g437120 63.198 394 107 3 27 418 40 397 2.64e-170 483
MS.gene073381.t1 MTR_2g437100 65.979 388 89 4 25 404 43 395 1.81e-169 481
MS.gene073381.t1 MTR_2g437960 63.351 382 103 2 38 417 53 399 1.56e-167 476
MS.gene073381.t1 MTR_2g437960 63.351 382 103 2 38 417 53 399 1.98e-167 476
MS.gene073381.t1 MTR_2g438010 59.569 418 123 3 13 419 36 418 9.22e-166 472
MS.gene073381.t1 MTR_2g437180 61.558 398 108 4 25 420 42 396 3.26e-165 469
MS.gene073381.t1 MTR_2g076320 59.905 419 122 3 12 419 15 398 3.43e-163 465
MS.gene073381.t1 MTR_2g437990 59.189 419 124 4 12 419 15 397 2.58e-161 461
MS.gene073381.t1 MTR_2g437130 55.000 400 134 4 21 419 32 386 1.67e-143 414
MS.gene073381.t1 MTR_2g437260 52.439 410 153 5 1 408 1 370 1.81e-143 414
MS.gene073381.t1 MTR_2g437940 56.927 397 100 4 25 419 42 369 1.51e-141 409
MS.gene073381.t1 MTR_2g437080 49.626 401 158 4 18 416 24 382 1.36e-130 381
MS.gene073381.t1 MTR_2g436440 49.626 401 158 4 18 416 30 388 3.93e-130 380
MS.gene073381.t1 MTR_2g437060 50.000 394 157 3 21 412 38 393 2.42e-127 373
MS.gene073381.t1 MTR_2g437020 50.882 397 148 6 20 412 25 378 7.29e-127 372
MS.gene073381.t1 MTR_2g437040 47.870 399 164 4 20 416 26 382 1.64e-121 358
MS.gene073381.t1 MTR_4g119550 47.244 381 162 3 33 412 46 388 3.66e-117 347
MS.gene073381.t1 MTR_2g437030 47.103 397 166 4 22 416 114 468 5.21e-116 347
MS.gene073381.t1 MTR_2g436400 45.209 407 176 6 7 408 17 381 5.28e-110 328
MS.gene073381.t1 MTR_2g436460 45.600 375 165 5 32 405 41 377 4.59e-100 303
MS.gene073381.t1 MTR_2g437160 73.936 188 49 0 233 420 133 320 4.80e-97 293
MS.gene073381.t1 MTR_2g437160 32.683 205 86 3 25 180 50 251 2.04e-23 100
MS.gene073381.t1 MTR_4g120380 43.514 370 171 2 33 402 39 370 1.89e-95 291
MS.gene073381.t1 MTR_4g119580 42.971 377 176 3 26 402 33 370 3.91e-95 290
MS.gene073381.t1 MTR_4g120380 44.000 350 158 2 33 382 39 350 7.91e-90 278
MS.gene073381.t1 MTR_2g019810 41.481 405 180 8 7 404 5 359 3.57e-82 257
MS.gene073381.t1 MTR_2g019840 39.950 398 197 6 16 411 21 378 4.90e-82 257
MS.gene073381.t1 MTR_2g437090 55.556 171 76 0 247 417 1 171 4.21e-65 206
MS.gene073381.t1 MTR_3g085240 46.371 248 72 7 1 243 21 212 1.20e-51 174
MS.gene073381.t1 MTR_3g085240 58.696 92 21 1 304 395 174 248 5.11e-25 103
MS.gene073381.t1 MTR_4g119570 38.889 252 118 2 151 402 14 229 2.13e-50 170
MS.gene073381.t1 MTR_3g450500 27.708 397 242 5 15 402 10 370 2.97e-47 166
MS.gene073381.t1 MTR_4g007490 28.997 369 223 4 37 402 36 368 5.17e-46 163
MS.gene073381.t1 MTR_2g436370 41.573 178 92 4 9 179 18 190 4.39e-32 121
MS.gene073381.t1 MTR_8g080990 28.012 332 200 4 69 398 74 368 1.11e-29 119
MS.gene073381.t1 MTR_8g081000 25.146 342 217 3 63 402 81 385 1.74e-28 115
MS.gene073381.t1 MTR_8g081000 25.146 342 217 3 63 402 81 385 2.14e-28 115
MS.gene073381.t1 MTR_8g081000 25.146 342 217 3 63 402 81 385 3.55e-28 115
MS.gene073381.t1 MTR_4g049400 41.732 127 67 2 25 144 37 163 5.35e-25 102
MS.gene073381.t1 MTR_2g436380 42.424 99 54 2 247 345 1 96 5.40e-16 78.2
MS.gene073381.t1 MTR_8g012210 20.065 309 238 5 118 418 63 370 1.84e-12 68.6
MS.gene073381.t1 MTR_4g119615 53.704 54 24 1 341 394 22 74 2.05e-11 60.5
MS.gene073381.t1 MTR_2g019830 56.452 62 26 1 344 405 140 200 8.05e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073381.t1 AT5G07900 30.750 400 236 4 8 401 35 399 5.99e-56 190
MS.gene073381.t1 AT1G21150 30.548 383 218 4 20 399 11 348 3.82e-48 168
MS.gene073381.t1 AT1G21150 30.685 365 215 3 38 399 55 384 1.66e-47 167
MS.gene073381.t1 AT1G21150 30.685 365 215 3 38 399 119 448 5.68e-47 167
MS.gene073381.t1 AT1G61980 27.179 390 205 7 37 387 50 399 1.12e-35 136
MS.gene073381.t1 AT1G61980 27.179 390 205 7 37 387 50 399 1.12e-35 136
MS.gene073381.t1 AT1G61980 27.179 390 205 7 37 387 50 399 1.12e-35 136
MS.gene073381.t1 AT1G61990 26.730 419 221 8 23 401 36 408 3.43e-34 132
MS.gene073381.t1 AT1G61990 26.730 419 221 8 23 401 36 408 3.43e-34 132
MS.gene073381.t1 AT1G61970 27.110 391 208 11 37 389 50 401 9.02e-34 131
MS.gene073381.t1 AT1G61970 27.110 391 208 11 37 389 50 401 9.02e-34 131
MS.gene073381.t1 AT1G61970 27.110 391 208 11 37 389 50 401 9.02e-34 131
MS.gene073381.t1 AT1G61970 27.110 391 208 11 37 389 50 401 9.02e-34 131
MS.gene073381.t1 AT1G61970 27.110 391 208 11 37 389 50 401 9.02e-34 131
MS.gene073381.t1 AT1G61970 27.110 391 208 11 37 389 50 401 9.02e-34 131
MS.gene073381.t1 AT1G61970 27.110 391 208 11 37 389 50 401 9.02e-34 131
MS.gene073381.t1 AT1G62120 25.714 385 208 5 22 364 33 381 7.28e-29 117
MS.gene073381.t1 AT3G46950 27.687 307 177 6 13 316 24 288 2.62e-28 116
MS.gene073381.t1 AT5G64950 24.810 395 241 8 23 402 23 376 4.94e-25 106
MS.gene073381.t1 AT1G62150 23.367 398 259 10 37 393 56 448 5.00e-24 103
MS.gene073381.t1 AT1G61960 28.525 305 175 6 17 316 27 293 1.12e-23 103
MS.gene073381.t1 AT5G23930 26.359 368 233 10 30 364 46 408 4.34e-22 98.6
MS.gene073381.t1 AT1G62085 25.693 397 234 11 39 420 55 405 5.33e-22 98.2
MS.gene073381.t1 AT1G62085 25.693 397 234 11 39 420 55 405 5.33e-22 98.2
MS.gene073381.t1 AT1G62110 27.500 280 164 6 39 316 52 294 1.03e-19 91.3
MS.gene073381.t1 AT1G56380 23.989 371 240 12 40 387 17 368 3.44e-18 86.3
MS.gene073381.t1 AT1G56380 23.989 371 240 12 40 387 28 379 3.67e-18 86.3
MS.gene073381.t1 AT1G79220 21.067 356 233 7 73 418 82 399 3.22e-12 68.2

Find 73 sgRNAs with CRISPR-Local

Find 97 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAACTTGATTTCACGAATTT+AGG 0.087763 2.4:+54531816 None:intergenic
CATCCTTCATTGACTACTTT+TGG 0.124797 2.4:-54531432 MS.gene073381:CDS
GTCAATTTGATGTCTGATTT+TGG 0.159011 2.4:-54531558 MS.gene073381:CDS
CGAACAAGGCCATCTATATT+TGG 0.170334 2.4:-54531504 MS.gene073381:CDS
CAAGTTTGTTTGCTCCTTTA+AGG 0.209354 2.4:-54531058 MS.gene073381:CDS
ATAATCAGATTCCTCCTTAA+AGG 0.220620 2.4:+54531044 None:intergenic
TAGAGGAAGTTAAGGGTTTA+GGG 0.232380 2.4:-54531458 MS.gene073381:CDS
TTAGAGGAAGTTAAGGGTTT+AGG 0.237816 2.4:-54531459 MS.gene073381:CDS
AGACCAGCCCCATTTGTTAA+AGG 0.263441 2.4:+54531350 None:intergenic
TGGCATGCCATTCACAAATT+AGG 0.295156 2.4:-54530968 None:intergenic
ATGCCATTCTTTGTTTAATT+CGG 0.306732 2.4:-54531629 MS.gene073381:CDS
AACTTGATTTCACGAATTTA+GGG 0.317503 2.4:+54531817 None:intergenic
AACTTCTCAGAATATCTAAA+TGG 0.329338 2.4:+54531090 None:intergenic
AAGCTAGATTTCAAAATTGT+AGG 0.330980 2.4:+54531711 None:intergenic
ACAAAACATCTGTGGTAGTT+TGG 0.331948 2.4:+54531206 None:intergenic
TCCTACCTCATTCACAAATT+CGG 0.344796 2.4:-54532083 MS.gene073381:CDS
TGCGGTTGAGGTTGTTGATA+TGG 0.356705 2.4:+54532175 None:intergenic
ATTGAAACCAACATCAAATT+AGG 0.361121 2.4:+54531294 None:intergenic
TAAGTCATTAGAGGAAGTTA+AGG 0.381295 2.4:-54531466 MS.gene073381:CDS
GACATTCTTCGTATGGTTGT+AGG 0.384943 2.4:-54531843 MS.gene073381:CDS
AAGAAGTTGAGAACTGAATC+TGG 0.385783 2.4:+54531999 None:intergenic
AGAATATTGGAGTGCGGTTG+AGG 0.397179 2.4:+54532163 None:intergenic
TCCTTTGAACCAAATATAGA+TGG 0.398078 2.4:+54531495 None:intergenic
ACAAAGAATGGCATCCTTGT+TGG 0.401490 2.4:+54531638 None:intergenic
AAGTTGACACCTTTAACAAA+TGG 0.405620 2.4:-54531359 MS.gene073381:CDS
ATCAGAGGAAGAAGCACCTT+TGG 0.406640 2.4:+54531758 None:intergenic
TGAGGAATCTTAGAGAATAT+TGG 0.425832 2.4:+54532150 None:intergenic
GGATTTGATTAAGTCATTAG+AGG 0.426999 2.4:-54531475 MS.gene073381:CDS
AAGTCATTAGAGGAAGTTAA+GGG 0.427528 2.4:-54531465 MS.gene073381:CDS
GCCATCTATATTTGGTTCAA+AGG 0.427799 2.4:-54531496 MS.gene073381:CDS
GAAATTACCTAATTTGTGAA+TGG 0.433382 2.4:+54530961 None:intergenic
AGTTGACACCTTTAACAAAT+GGG 0.435374 2.4:-54531358 MS.gene073381:CDS
CTCAACTTCTTCAAAAGTCA+TGG 0.435992 2.4:-54531987 MS.gene073381:CDS
TCAATTGGGTTGGAATTCCT+TGG 0.440659 2.4:-54531235 MS.gene073381:CDS
GTCAGAGGTAGAAGCACCTT+TGG 0.453352 2.4:+54531863 None:intergenic
AGCTAGATTTCAAAATTGTA+GGG 0.454084 2.4:+54531712 None:intergenic
TACCTCTGACATTCTTCGTA+TGG 0.462578 2.4:-54531850 MS.gene073381:CDS
TTTCATGCAATGCCCACAAA+AGG 0.467899 2.4:-54531914 MS.gene073381:CDS
ATGCCGAATTAAACAAAGAA+TGG 0.473359 2.4:+54531626 None:intergenic
ATTATCCACAGTAAGGTGAT+AGG 0.484709 2.4:+54531581 None:intergenic
TCTAAATGGTGTAACTAAGC+TGG 0.495852 2.4:+54531104 None:intergenic
TTTGGGTCAATCAATTGGGT+TGG 0.506924 2.4:-54531245 MS.gene073381:CDS
GTTGCAGCCTAATTTGATGT+TGG 0.507199 2.4:-54531301 MS.gene073381:CDS
AACTGCATAACTGAGGCCCT+TGG 0.522658 2.4:+54531159 None:intergenic
ACACCTTTAACAAATGGGGC+TGG 0.542117 2.4:-54531353 MS.gene073381:CDS
ACCCCAAAAGTAGTCAATGA+AGG 0.557831 2.4:+54531429 None:intergenic
TTGCTAGATTGCTTCGAACA+AGG 0.560619 2.4:-54531518 MS.gene073381:CDS
AATTGACATTATCCACAGTA+AGG 0.560808 2.4:+54531574 None:intergenic
TGGAGAAAAGGATCATTCCA+AGG 0.570897 2.4:-54531176 MS.gene073381:CDS
TGGTAGTTTGGCAAGTGCCA+AGG 0.586106 2.4:+54531218 None:intergenic
TGAGGTTGTTGATATGGCAA+AGG 0.590012 2.4:+54532181 None:intergenic
GTTTGTTTGCTCCTTTAAGG+AGG 0.603996 2.4:-54531055 MS.gene073381:CDS
AGAAACCGAATTTGTGAATG+AGG 0.604803 2.4:+54532078 None:intergenic
ACCGAATTTGTGAATGAGGT+AGG 0.607992 2.4:+54532082 None:intergenic
AATGAAACTTGCATACACAA+TGG 0.609213 2.4:-54530998 MS.gene073381:CDS
TGAATTTGAATCTGAAGCGA+TGG 0.615539 2.4:+54532121 None:intergenic
TAACAATGCAACAATATCAG+AGG 0.616711 2.4:+54531743 None:intergenic
TGAATCTGAAGCGATGGTAG+TGG 0.621211 2.4:+54532127 None:intergenic
GTTGACACCTTTAACAAATG+GGG 0.635802 2.4:-54531357 MS.gene073381:CDS
GCATTGCATGAAAGAAGCCA+TGG 0.636869 2.4:+54531924 None:intergenic
AAACTAAAACAAAACATCTG+TGG 0.637333 2.4:+54531198 None:intergenic
GGAGAAAAGGATCATTCCAA+GGG 0.639024 2.4:-54531175 MS.gene073381:CDS
TTGATATGGCAAAGGCCTTG+CGG 0.640767 2.4:+54532189 None:intergenic
GGTATTCATAGAAAATGCAG+TGG 0.641012 2.4:+54531602 None:intergenic
GCATACACAATGGAAAACAA+TGG 0.644592 2.4:-54530988 MS.gene073381:CDS
CTGAAGCGATGGTAGTGGTG+AGG 0.647119 2.4:+54532132 None:intergenic
TCTTAGAGAATATTGGAGTG+CGG 0.670463 2.4:+54532157 None:intergenic
AACCATACGAAGAATGTCAG+AGG 0.672395 2.4:+54531848 None:intergenic
GAATACCTATCACCTTACTG+TGG 0.678196 2.4:-54531586 MS.gene073381:CDS
CAAAAGAAACTGCATAACTG+AGG 0.684507 2.4:+54531152 None:intergenic
GCATCATCAAAAGAGAACCA+TGG 0.694296 2.4:-54531941 MS.gene073381:CDS
TAGGTTTCTGAAAACCAACA+AGG 0.701717 2.4:-54531652 MS.gene073381:CDS
TTATCTATTAAAGCTATACG+AGG 0.742253 2.4:-54531025 MS.gene073381:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TACTTAAAAATTCAAAAAGA+GGG + chr2.4:54531508-54531527 None:intergenic 15.0%
!! TGATTAAAAATTGAAACTTT+GGG + chr2.4:54531295-54531314 None:intergenic 15.0%
!! AATGTATGAGTAAAAAACTT+TGG - chr2.4:54531789-54531808 MS.gene073381:CDS 20.0%
!! ATGTATGAGTAAAAAACTTT+GGG - chr2.4:54531790-54531809 MS.gene073381:CDS 20.0%
!! CTACTTAAAAATTCAAAAAG+AGG + chr2.4:54531509-54531528 None:intergenic 20.0%
!! GTGATTAAAAATTGAAACTT+TGG + chr2.4:54531296-54531315 None:intergenic 20.0%
!!! CTCTTTTTGAATTTTTAAGT+AGG - chr2.4:54531507-54531526 MS.gene073381:CDS 20.0%
!!! TTTCAATTTTTAATCACCAA+AGG - chr2.4:54531299-54531318 MS.gene073381:CDS 20.0%
!!! TTTCATTTTTTTCTATCCAA+AGG - chr2.4:54531404-54531423 MS.gene073381:CDS 20.0%
! AAACTAAAACAAAACATCTG+TGG + chr2.4:54531983-54532002 None:intergenic 25.0%
! AAACTTGATTTCACGAATTT+AGG + chr2.4:54531365-54531384 None:intergenic 25.0%
! AACTTCTCAGAATATCTAAA+TGG + chr2.4:54532091-54532110 None:intergenic 25.0%
! AACTTGATTTCACGAATTTA+GGG + chr2.4:54531364-54531383 None:intergenic 25.0%
! AAGCTAGATTTCAAAATTGT+AGG + chr2.4:54531470-54531489 None:intergenic 25.0%
! AGCTAGATTTCAAAATTGTA+GGG + chr2.4:54531469-54531488 None:intergenic 25.0%
! ATTGAAACCAACATCAAATT+AGG + chr2.4:54531887-54531906 None:intergenic 25.0%
! TTATCTATTAAAGCTATACG+AGG - chr2.4:54532153-54532172 MS.gene073381:CDS 25.0%
!! ATGCCATTCTTTGTTTAATT+CGG - chr2.4:54531549-54531568 MS.gene073381:CDS 25.0%
!! TGATTCGATAATGAGTTTTT+GGG - chr2.4:54531916-54531935 MS.gene073381:CDS 25.0%
!!! ACAGATGTTTTGTTTTAGTT+TGG - chr2.4:54531982-54532001 MS.gene073381:CDS 25.0%
!!! TACAATTTTGAAATCTAGCT+TGG - chr2.4:54531469-54531488 MS.gene073381:CDS 25.0%
!!! TTGATTCGATAATGAGTTTT+TGG - chr2.4:54531915-54531934 MS.gene073381:CDS 25.0%
!!! TTTGTTTTAGTTTGGAGAAA+AGG - chr2.4:54531990-54532009 MS.gene073381:CDS 25.0%
!!! TTTTTTACTCATACATTGCT+TGG + chr2.4:54531786-54531805 None:intergenic 25.0%
AAGTCATTAGAGGAAGTTAA+GGG - chr2.4:54531713-54531732 MS.gene073381:CDS 30.0%
AAGTTGACACCTTTAACAAA+TGG - chr2.4:54531819-54531838 MS.gene073381:CDS 30.0%
AATGAAACTTGCATACACAA+TGG - chr2.4:54532180-54532199 MS.gene073381:CDS 30.0%
AATTGACATTATCCACAGTA+AGG + chr2.4:54531607-54531626 None:intergenic 30.0%
AGTTGACACCTTTAACAAAT+GGG - chr2.4:54531820-54531839 MS.gene073381:CDS 30.0%
ATAATCAGATTCCTCCTTAA+AGG + chr2.4:54532137-54532156 None:intergenic 30.0%
ATGCCGAATTAAACAAAGAA+TGG + chr2.4:54531555-54531574 None:intergenic 30.0%
GGATTTGATTAAGTCATTAG+AGG - chr2.4:54531703-54531722 MS.gene073381:CDS 30.0%
TAACAATGCAACAATATCAG+AGG + chr2.4:54531438-54531457 None:intergenic 30.0%
TAAGTCATTAGAGGAAGTTA+AGG - chr2.4:54531712-54531731 MS.gene073381:CDS 30.0%
TCCTTTGAACCAAATATAGA+TGG + chr2.4:54531686-54531705 None:intergenic 30.0%
TGAGGAATCTTAGAGAATAT+TGG + chr2.4:54531031-54531050 None:intergenic 30.0%
! ATGCAGTTTCTTTTGATGAA+AGG - chr2.4:54532034-54532053 MS.gene073381:CDS 30.0%
! GTCAATTTGATGTCTGATTT+TGG - chr2.4:54531620-54531639 MS.gene073381:CDS 30.0%
!! ATCCTTCATTGACTACTTTT+GGG - chr2.4:54531747-54531766 MS.gene073381:CDS 30.0%
!!! AGTTTTTGGGTCAATCAATT+GGG - chr2.4:54531929-54531948 MS.gene073381:CDS 30.0%
!!! GGTTTTGAAACGAAGATTTT+TGG + chr2.4:54531150-54531169 None:intergenic 30.0%
AAGAAGTTGAGAACTGAATC+TGG + chr2.4:54531182-54531201 None:intergenic 35.0%
ACAAAACATCTGTGGTAGTT+TGG + chr2.4:54531975-54531994 None:intergenic 35.0%
AGAAACCGAATTTGTGAATG+AGG + chr2.4:54531103-54531122 None:intergenic 35.0%
ATTATCCACAGTAAGGTGAT+AGG + chr2.4:54531600-54531619 None:intergenic 35.0%
CAAAAGAAACTGCATAACTG+AGG + chr2.4:54532029-54532048 None:intergenic 35.0%
CTCAACTTCTTCAAAAGTCA+TGG - chr2.4:54531191-54531210 MS.gene073381:CDS 35.0%
GCATACACAATGGAAAACAA+TGG - chr2.4:54532190-54532209 MS.gene073381:CDS 35.0%
GGTATTCATAGAAAATGCAG+TGG + chr2.4:54531579-54531598 None:intergenic 35.0%
GTTGACACCTTTAACAAATG+GGG - chr2.4:54531821-54531840 MS.gene073381:CDS 35.0%
TAGAGGAAGTTAAGGGTTTA+GGG - chr2.4:54531720-54531739 MS.gene073381:CDS 35.0%
TCCTACCTCATTCACAAATT+CGG - chr2.4:54531095-54531114 MS.gene073381:CDS 35.0%
TCTAAATGGTGTAACTAAGC+TGG + chr2.4:54532077-54532096 None:intergenic 35.0%
TCTTAGAGAATATTGGAGTG+CGG + chr2.4:54531024-54531043 None:intergenic 35.0%
TTAGAGGAAGTTAAGGGTTT+AGG - chr2.4:54531719-54531738 MS.gene073381:CDS 35.0%
! CAAGTTTGTTTGCTCCTTTA+AGG - chr2.4:54532120-54532139 MS.gene073381:CDS 35.0%
! GCCATCTATATTTGGTTCAA+AGG - chr2.4:54531682-54531701 MS.gene073381:CDS 35.0%
! TAGGTTTCTGAAAACCAACA+AGG - chr2.4:54531526-54531545 MS.gene073381:CDS 35.0%
! TGAATTTGAATCTGAAGCGA+TGG + chr2.4:54531060-54531079 None:intergenic 35.0%
!! CATCCTTCATTGACTACTTT+TGG - chr2.4:54531746-54531765 MS.gene073381:CDS 35.0%
!! GAAACTTTTTCTATTCCGCA+AGG - chr2.4:54530974-54530993 MS.gene073381:CDS 35.0%
!! TCCTTCATTGACTACTTTTG+GGG - chr2.4:54531748-54531767 MS.gene073381:CDS 35.0%
!!! AACTGAATCTGGTTTTTGTG+AGG + chr2.4:54531171-54531190 None:intergenic 35.0%
!!! GAGTTTTTGGGTCAATCAAT+TGG - chr2.4:54531928-54531947 MS.gene073381:CDS 35.0%
AACCATACGAAGAATGTCAG+AGG + chr2.4:54531333-54531352 None:intergenic 40.0%
ACAAAGAATGGCATCCTTGT+TGG + chr2.4:54531543-54531562 None:intergenic 40.0%
ACCCCAAAAGTAGTCAATGA+AGG + chr2.4:54531752-54531771 None:intergenic 40.0%
ACCGAATTTGTGAATGAGGT+AGG + chr2.4:54531099-54531118 None:intergenic 40.0%
CGAACAAGGCCATCTATATT+TGG - chr2.4:54531674-54531693 MS.gene073381:CDS 40.0%
GAATACCTATCACCTTACTG+TGG - chr2.4:54531592-54531611 MS.gene073381:CDS 40.0%
GACATTCTTCGTATGGTTGT+AGG - chr2.4:54531335-54531354 MS.gene073381:CDS 40.0%
GCATCATCAAAAGAGAACCA+TGG - chr2.4:54531237-54531256 MS.gene073381:CDS 40.0%
GGAGAAAAGGATCATTCCAA+GGG - chr2.4:54532003-54532022 MS.gene073381:CDS 40.0%
GTTGCAGCCTAATTTGATGT+TGG - chr2.4:54531877-54531896 MS.gene073381:CDS 40.0%
TACCTCTGACATTCTTCGTA+TGG - chr2.4:54531328-54531347 MS.gene073381:CDS 40.0%
TGAGGTTGTTGATATGGCAA+AGG + chr2.4:54531000-54531019 None:intergenic 40.0%
TGGAGAAAAGGATCATTCCA+AGG - chr2.4:54532002-54532021 MS.gene073381:CDS 40.0%
TTGCTAGATTGCTTCGAACA+AGG - chr2.4:54531660-54531679 MS.gene073381:CDS 40.0%
TTTCATGCAATGCCCACAAA+AGG - chr2.4:54531264-54531283 MS.gene073381:CDS 40.0%
TTTGGGTCAATCAATTGGGT+TGG - chr2.4:54531933-54531952 MS.gene073381:CDS 40.0%
! GTTTGTTTGCTCCTTTAAGG+AGG - chr2.4:54532123-54532142 MS.gene073381:CDS 40.0%
!! ACTTTGGGAGAATCCTTTTG+TGG + chr2.4:54531280-54531299 None:intergenic 40.0%
!! CTTTGGGAGAATCCTTTTGT+GGG + chr2.4:54531279-54531298 None:intergenic 40.0%
!! TCAATTGGGTTGGAATTCCT+TGG - chr2.4:54531943-54531962 MS.gene073381:CDS 40.0%
ACACCTTTAACAAATGGGGC+TGG - chr2.4:54531825-54531844 MS.gene073381:CDS 45.0%
AGAATATTGGAGTGCGGTTG+AGG + chr2.4:54531018-54531037 None:intergenic 45.0%
GCATTGCATGAAAGAAGCCA+TGG + chr2.4:54531257-54531276 None:intergenic 45.0%
TGCGGTTGAGGTTGTTGATA+TGG + chr2.4:54531006-54531025 None:intergenic 45.0%
TTGATATGGCAAAGGCCTTG+CGG + chr2.4:54530992-54531011 None:intergenic 45.0%
! AGACCAGCCCCATTTGTTAA+AGG + chr2.4:54531831-54531850 None:intergenic 45.0%
! TGAATCTGAAGCGATGGTAG+TGG + chr2.4:54531054-54531073 None:intergenic 45.0%
!! ATCAGAGGAAGAAGCACCTT+TGG + chr2.4:54531423-54531442 None:intergenic 45.0%
!!! GACTACTTTTGGGGTTGCTT+TGG - chr2.4:54531757-54531776 MS.gene073381:CDS 45.0%
AACTGCATAACTGAGGCCCT+TGG + chr2.4:54532022-54532041 None:intergenic 50.0%
TGGTAGTTTGGCAAGTGCCA+AGG + chr2.4:54531963-54531982 None:intergenic 50.0%
!! GTCAGAGGTAGAAGCACCTT+TGG + chr2.4:54531318-54531337 None:intergenic 50.0%
!! CTGAAGCGATGGTAGTGGTG+AGG + chr2.4:54531049-54531068 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 54530969 54532231 54530969 ID=MS.gene073381
chr2.4 mRNA 54530969 54532231 54530969 ID=MS.gene073381.t1;Parent=MS.gene073381
chr2.4 exon 54530969 54532231 54530969 ID=MS.gene073381.t1.exon1;Parent=MS.gene073381.t1
chr2.4 CDS 54530969 54532231 54530969 ID=cds.MS.gene073381.t1;Parent=MS.gene073381.t1
Gene Sequence

>MS.gene073381

ATGTTGAAACTTTTTCTATTCCGCAAGGCCTTTGCCATATCAACAACCTCAACCGCACTCCAATATTCTCTAAGATTCCTCACCACTACCATCGCTTCAGATTCAAATTCAAATTCATTCGCTGTATCCTACCTCATTCACAAATTCGGTTTCTCACATCAATTCGCACTCAAAGCTTCCAAAAATCTTCGTTTCAAAACCTCACAAAAACCAGATTCAGTTCTCAACTTCTTCAAAAGTCATGGTTTCACTGACTCAGATATACACAGCATCATCAAAAGAGAACCATGGCTTCTTTCATGCAATGCCCACAAAAGGATTCTCCCAAAGTTTCAATTTTTAATCACCAAAGGTGCTTCTACCTCTGACATTCTTCGTATGGTTGTAGGTAACCCTAAATTCGTGAAATCAAGTTTGAAGAATCATGAAACTATGTTTCATTTTTTTCTATCCAAAGGTGCTTCTTCCTCTGATATTGTTGCATTGTTAACTGCTAACCCTACAATTTTGAAATCTAGCTTGGAGAGACGAATAATCCCTCTTTTTGAATTTTTAAGTAGGTTTCTGAAAACCAACAAGGATGCCATTCTTTGTTTAATTCGGCATTCCACTGCATTTTCTATGAATACCTATCACCTTACTGTGGATAATGTCAATTTGATGTCTGATTTTGGAGTTTCTGATTCTACAATTGCTAGATTGCTTCGAACAAGGCCATCTATATTTGGTTCAAAGGATTTGATTAAGTCATTAGAGGAAGTTAAGGGTTTAGGGTTTCATCCTTCATTGACTACTTTTGGGGTTGCTTTGGTAGCCAAGCAATGTATGAGTAAAAAACTTTGGGATGAGAAAGTTGACACCTTTAACAAATGGGGCTGGTCTGATGAAGCTGTTATTCAAACATTTCAGTTGCAGCCTAATTTGATGTTGGTTTCAATTGATAAGATTGATTCGATAATGAGTTTTTGGGTCAATCAATTGGGTTGGAATTCCTTGGCACTTGCCAAACTACCACAGATGTTTTGTTTTAGTTTGGAGAAAAGGATCATTCCAAGGGCCTCAGTTATGCAGTTTCTTTTGATGAAAGGTTTGCGAAAAAAGAATGCCAGCTTAGTTACACCATTTAGATATTCTGAGAAGTTGTTCTTGAACAAGTTTGTTTGCTCCTTTAAGGAGGAATCTGATTATCTATTAAAGCTATACGAGGAAAAAATGAAACTTGCATACACAATGGAAAACAATGGCATGCCATTCACAAATTAG
Protein sequence

>MS.gene073381.t1

MLKLFLFRKAFAISTTSTALQYSLRFLTTTIASDSNSNSFAVSYLIHKFGFSHQFALKASKNLRFKTSQKPDSVLNFFKSHGFTDSDIHSIIKREPWLLSCNAHKRILPKFQFLITKGASTSDILRMVVGNPKFVKSSLKNHETMFHFFLSKGASSSDIVALLTANPTILKSSLERRIIPLFEFLSRFLKTNKDAILCLIRHSTAFSMNTYHLTVDNVNLMSDFGVSDSTIARLLRTRPSIFGSKDLIKSLEEVKGLGFHPSLTTFGVALVAKQCMSKKLWDEKVDTFNKWGWSDEAVIQTFQLQPNLMLVSIDKIDSIMSFWVNQLGWNSLALAKLPQMFCFSLEKRIIPRASVMQFLLMKGLRKKNASLVTPFRYSEKLFLNKFVCSFKEESDYLLKLYEEKMKLAYTMENNGMPFTN