AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene073435

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073435.t1 MTR_2g438020 79.481 385 78 1 29 413 70 453 0.0 611
MS.gene073435.t1 MTR_2g437240 72.182 417 112 2 1 414 1 416 0.0 598
MS.gene073435.t1 MTR_2g437200 76.633 398 91 1 1 396 1 398 0.0 597
MS.gene073435.t1 MTR_2g437960 65.116 387 98 2 30 414 53 404 2.54e-176 498
MS.gene073435.t1 MTR_2g437960 65.285 386 97 2 30 413 53 403 1.56e-175 497
MS.gene073435.t1 MTR_2g437120 64.359 390 101 3 22 409 43 396 1.61e-168 479
MS.gene073435.t1 MTR_2g076320 62.657 399 109 3 23 416 40 403 1.10e-166 473
MS.gene073435.t1 MTR_2g437990 63.131 396 105 4 23 413 40 399 1.99e-166 474
MS.gene073435.t1 MTR_2g438010 61.461 397 113 3 23 414 60 421 2.91e-166 473
MS.gene073435.t1 MTR_2g437170 61.139 404 112 5 21 414 42 410 4.61e-164 467
MS.gene073435.t1 MTR_2g437150 61.153 399 112 4 21 411 42 405 6.12e-163 464
MS.gene073435.t1 MTR_2g437180 62.374 396 102 5 21 411 42 395 5.81e-162 461
MS.gene073435.t1 MTR_2g437160 60.406 394 114 4 21 408 50 407 3.26e-157 450
MS.gene073435.t1 MTR_2g437940 60.302 398 91 4 21 416 42 374 1.67e-154 441
MS.gene073435.t1 MTR_2g437100 60.567 388 106 4 21 396 43 395 3.78e-152 437
MS.gene073435.t1 MTR_2g437130 55.527 389 133 3 25 412 38 387 1.04e-138 402
MS.gene073435.t1 MTR_2g437260 47.922 409 167 6 1 401 1 371 1.43e-123 363
MS.gene073435.t1 MTR_2g437020 50.000 394 154 5 20 409 29 383 6.04e-123 362
MS.gene073435.t1 MTR_2g437060 48.711 388 159 3 19 404 44 393 1.13e-121 358
MS.gene073435.t1 MTR_2g437080 46.939 392 168 3 19 408 29 382 1.88e-121 358
MS.gene073435.t1 MTR_2g436440 46.898 403 174 3 9 409 25 389 8.46e-119 351
MS.gene073435.t1 MTR_2g437030 45.293 393 175 3 19 409 115 469 3.96e-113 340
MS.gene073435.t1 MTR_4g119550 45.407 381 169 3 25 404 46 388 1.50e-111 333
MS.gene073435.t1 MTR_2g436400 45.812 382 165 5 22 400 39 381 6.68e-111 331
MS.gene073435.t1 MTR_2g437040 45.545 404 173 5 8 408 23 382 2.28e-110 329
MS.gene073435.t1 MTR_2g436460 47.200 375 159 4 24 397 41 377 9.79e-102 307
MS.gene073435.t1 MTR_4g120380 44.054 370 169 2 24 393 38 369 6.31e-97 295
MS.gene073435.t1 MTR_4g120380 44.444 351 157 2 24 374 38 350 6.40e-92 283
MS.gene073435.t1 MTR_4g119580 43.094 362 168 2 32 393 46 369 7.52e-89 274
MS.gene073435.t1 MTR_2g437160 64.623 212 72 1 200 408 105 316 3.30e-88 271
MS.gene073435.t1 MTR_2g437160 31.707 205 84 4 21 172 50 251 5.96e-20 90.5
MS.gene073435.t1 MTR_2g019810 42.667 375 163 7 29 396 30 359 3.62e-83 259
MS.gene073435.t1 MTR_2g019840 39.633 381 186 7 24 401 37 376 8.71e-80 251
MS.gene073435.t1 MTR_2g437090 55.422 166 74 0 239 404 1 166 1.77e-59 191
MS.gene073435.t1 MTR_4g119570 40.000 250 114 2 143 392 14 227 1.35e-52 176
MS.gene073435.t1 MTR_3g450500 30.055 366 216 4 32 393 40 369 6.49e-50 173
MS.gene073435.t1 MTR_4g007490 30.055 366 215 5 32 393 39 367 1.14e-46 164
MS.gene073435.t1 MTR_3g085240 46.392 194 53 4 1 186 21 171 3.40e-37 136
MS.gene073435.t1 MTR_3g085240 55.556 90 23 1 298 387 176 248 2.78e-22 95.9
MS.gene073435.t1 MTR_2g436370 38.728 173 69 2 21 191 37 174 1.47e-30 117
MS.gene073435.t1 MTR_8g080990 27.928 333 199 5 61 390 74 368 3.13e-29 117
MS.gene073435.t1 MTR_8g081000 26.039 361 227 6 59 416 85 408 3.57e-29 118
MS.gene073435.t1 MTR_8g081000 26.706 337 208 5 59 393 85 384 7.73e-29 117
MS.gene073435.t1 MTR_8g081000 26.706 337 208 5 59 393 85 384 8.90e-29 116
MS.gene073435.t1 MTR_4g049400 46.875 128 57 3 21 137 37 164 1.96e-28 112
MS.gene073435.t1 MTR_2g436380 58.333 60 25 0 239 298 1 60 9.30e-17 80.5
MS.gene073435.t1 MTR_5g068860 20.923 325 209 6 67 348 143 462 4.00e-11 65.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene073435.t1 AT5G07900 31.250 384 216 5 23 393 51 399 2.87e-57 193
MS.gene073435.t1 AT1G21150 30.303 363 215 3 32 391 57 384 2.09e-46 164
MS.gene073435.t1 AT1G21150 30.303 363 215 3 32 391 121 448 6.85e-46 164
MS.gene073435.t1 AT1G21150 29.781 366 219 3 29 391 18 348 1.75e-45 161
MS.gene073435.t1 AT1G61990 25.874 429 230 7 8 393 25 408 2.80e-33 129
MS.gene073435.t1 AT1G61990 25.874 429 230 7 8 393 25 408 2.80e-33 129
MS.gene073435.t1 AT1G61970 27.226 393 195 11 32 379 53 399 2.41e-31 124
MS.gene073435.t1 AT1G61970 27.226 393 195 11 32 379 53 399 2.41e-31 124
MS.gene073435.t1 AT1G61970 27.226 393 195 11 32 379 53 399 2.41e-31 124
MS.gene073435.t1 AT1G61970 27.226 393 195 11 32 379 53 399 2.41e-31 124
MS.gene073435.t1 AT1G61970 27.226 393 195 11 32 379 53 399 2.41e-31 124
MS.gene073435.t1 AT1G61970 27.226 393 195 11 32 379 53 399 2.41e-31 124
MS.gene073435.t1 AT1G61970 27.226 393 195 11 32 379 53 399 2.41e-31 124
MS.gene073435.t1 AT1G62120 26.923 364 194 6 29 356 54 381 4.51e-31 124
MS.gene073435.t1 AT1G61980 27.390 387 202 8 32 379 53 399 1.23e-30 122
MS.gene073435.t1 AT1G61980 27.390 387 202 8 32 379 53 399 1.23e-30 122
MS.gene073435.t1 AT1G61980 27.390 387 202 8 32 379 53 399 1.23e-30 122
MS.gene073435.t1 AT5G64950 27.177 379 232 6 24 393 32 375 9.51e-29 116
MS.gene073435.t1 AT3G46950 28.070 285 154 6 32 311 53 291 3.91e-24 104
MS.gene073435.t1 AT1G61960 28.417 278 161 3 32 308 53 293 5.88e-24 103
MS.gene073435.t1 AT1G62150 26.573 286 157 4 32 308 59 300 1.12e-23 103
MS.gene073435.t1 AT1G62085 27.554 323 172 7 32 339 56 331 9.12e-23 100
MS.gene073435.t1 AT1G62085 27.554 323 172 7 32 339 56 331 9.12e-23 100
MS.gene073435.t1 AT1G62110 25.784 287 160 4 32 309 53 295 3.72e-21 95.5
MS.gene073435.t1 AT1G62010 24.857 350 186 6 64 376 71 380 4.19e-21 95.1
MS.gene073435.t1 AT5G23930 22.892 415 206 9 32 375 56 427 3.81e-17 83.6
MS.gene073435.t1 AT1G56380 25.474 369 237 14 32 377 28 381 1.58e-13 72.0
MS.gene073435.t1 AT1G56380 25.474 369 237 14 32 377 17 370 1.65e-13 72.0
MS.gene073435.t1 AT2G44020 20.708 367 266 8 9 357 82 441 3.36e-13 71.6

Find 76 sgRNAs with CRISPR-Local

Find 98 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CACTCAAGGCCATCTATATT+TGG 0.148576 2.4:-53787462 MS.gene073435:CDS
GATCCTTCATTGACTACTTT+TGG 0.148781 2.4:-53787390 MS.gene073435:CDS
ATCAGTTTGATGACTGATTT+TGG 0.150667 2.4:-53787516 MS.gene073435:CDS
TTAATTTGGTGATGAGATTT+TGG 0.155421 2.4:-53787221 MS.gene073435:CDS
TTCAATTGATAAGATTAATT+TGG 0.211307 2.4:-53787235 MS.gene073435:CDS
TTAGTTATAGTTTACATAAA+AGG 0.215972 2.4:-53787146 MS.gene073435:CDS
AAACTATAACTAAACATATT+TGG 0.236813 2.4:+53787156 None:intergenic
TGGAGGAAGTAAAGAGTTTA+GGG 0.240494 2.4:-53787416 MS.gene073435:CDS
TTGGAGGAAGTAAAGAGTTT+AGG 0.267899 2.4:-53787417 MS.gene073435:CDS
TTTCTCGGTCGTAATGCTTT+TGG 0.287185 2.4:-53788131 MS.gene073435:CDS
AACTGATTTGATTAAATCAT+TGG 0.289242 2.4:-53787436 MS.gene073435:CDS
ATTGAAGTCAACATCAAATT+AGG 0.302705 2.4:+53787252 None:intergenic
ATGAGGTAGGATACGGTAAA+TGG 0.313971 2.4:+53788053 None:intergenic
AAACCGTTATTCGAGTATTT+AGG 0.319173 2.4:-53787281 MS.gene073435:CDS
AAGCTAATGTGTAAAATTGT+AGG 0.327956 2.4:+53787669 None:intergenic
TAGAGGTAACCCTCAGTTCT+TGG 0.332921 2.4:-53787784 MS.gene073435:CDS
ATTTGATGTCTTTAAGAAAT+GGG 0.334222 2.4:-53787316 MS.gene073435:CDS
GAAGCTTTGAGTGCGGATTC+AGG 0.344614 2.4:+53788011 None:intergenic
AATTTGATGTCTTTAAGAAA+TGG 0.359065 2.4:-53787317 MS.gene073435:CDS
CGATTCTCAGAATAAGTAAA+TGG 0.361831 2.4:+53787048 None:intergenic
GTCAGATTCAGAAGCACCTT+TGG 0.364937 2.4:+53787821 None:intergenic
AGATCAAAATCTTATACATT+TGG 0.374741 2.4:+53786912 None:intergenic
AAGAAGTTGAGAACTGAATC+AGG 0.382302 2.4:+53787957 None:intergenic
GACATTGTCCGCATGGTTAG+AGG 0.397781 2.4:-53787801 MS.gene073435:CDS
ATCAGATGAAGAAGCACCTT+TGG 0.404344 2.4:+53787716 None:intergenic
TAGTTATAGTTTACATAAAA+GGG 0.406121 2.4:-53787145 MS.gene073435:CDS
TCATTTAGGTTGGGATTCCT+TGG 0.419185 2.4:-53787193 MS.gene073435:CDS
TTGGGTCAATCATTTAGGTT+GGG 0.436055 2.4:-53787202 MS.gene073435:CDS
TCAGTTGAACCAAATATAGA+TGG 0.441181 2.4:+53787453 None:intergenic
TCAATGTTGAAAGCATTTCT+CGG 0.441510 2.4:-53788146 MS.gene073435:CDS
CATTTACTTATTCTGAGAAT+CGG 0.448897 2.4:-53787047 MS.gene073435:CDS
TCAAAATCTTATACATTTGG+TGG 0.453156 2.4:+53786915 None:intergenic
TTTCAGCTTTAAGGAGGAAT+CGG 0.464344 2.4:-53787007 MS.gene073435:CDS
ACATACGCAATATCATCAAA+AGG 0.472800 2.4:-53787908 MS.gene073435:CDS
CAAATACAACGGAGAACAAT+GGG 0.473276 2.4:-53786945 MS.gene073435:CDS
TTTGGGTCAATCATTTAGGT+TGG 0.474131 2.4:-53787203 MS.gene073435:CDS
AGCTAATGTGTAAAATTGTA+GGG 0.492224 2.4:+53787670 None:intergenic
TACTCGAATAACGGTTTCAT+CGG 0.500961 2.4:+53787287 None:intergenic
AGCTTAATTCCAAGAACTGA+GGG 0.508231 2.4:+53787775 None:intergenic
TTTGATGTCTTTAAGAAATG+GGG 0.510130 2.4:-53787315 MS.gene073435:CDS
AATTGCAAAACTGAGGCCCT+TGG 0.520839 2.4:+53787117 None:intergenic
GTGAAGAGGTGGTAGTGGTA+AGG 0.521046 2.4:+53788078 None:intergenic
TACATAAAAGGGTCATTCCA+AGG 0.527481 2.4:-53787134 MS.gene073435:CDS
TGAGGTAGGATACGGTAAAT+GGG 0.529833 2.4:+53788054 None:intergenic
CATACGCAATATCATCAAAA+GGG 0.549145 2.4:-53787907 MS.gene073435:CDS
TGGAAATTTAGCAAGTGCCA+AGG 0.550727 2.4:+53787176 None:intergenic
GCCCCAAAAGTAGTCAATGA+AGG 0.551784 2.4:+53787387 None:intergenic
CTCTTTGGAAGCTTTGAGTG+CGG 0.552416 2.4:+53788004 None:intergenic
GTAGGGTTTGAAGTTAACAA+TGG 0.555837 2.4:+53787687 None:intergenic
AACTGATATTAGCCACCGTA+AGG 0.557576 2.4:+53787532 None:intergenic
TTGCTAGATTGCTTCACTCA+AGG 0.565363 2.4:-53787476 MS.gene073435:CDS
TACGGTAAATGGGTGTGAAG+AGG 0.567945 2.4:+53788064 None:intergenic
TGATTTGATTAAATCATTGG+AGG 0.568194 2.4:-53787433 MS.gene073435:CDS
GCAAATACAACGGAGAACAA+TGG 0.572984 2.4:-53786946 MS.gene073435:CDS
TTCAAATTTGTCGATGAGGT+AGG 0.573096 2.4:+53788040 None:intergenic
TGAATCTGACATTGTCCGCA+TGG 0.573592 2.4:-53787808 MS.gene073435:CDS
GCTGCTTTGAAAGCCAAGAA+AGG 0.575078 2.4:-53787366 MS.gene073435:CDS
AGACCTAAATACTCGAATAA+CGG 0.575195 2.4:+53787278 None:intergenic
GGTAAATGGGTGTGAAGAGG+TGG 0.576624 2.4:+53788067 None:intergenic
TTTGTCGATGAGGTAGGATA+CGG 0.586173 2.4:+53788046 None:intergenic
TATTAGCCACCGTAAGGCGA+TGG 0.602409 2.4:+53787538 None:intergenic
ACATAAAAGGGTCATTCCAA+GGG 0.603881 2.4:-53787133 MS.gene073435:CDS
AGAATTCAAATTTGTCGATG+AGG 0.604058 2.4:+53788036 None:intergenic
AAGCTTAATTCCAAGAACTG+AGG 0.606825 2.4:+53787774 None:intergenic
TGGGTGTGAAGAGGTGGTAG+TGG 0.611966 2.4:+53788073 None:intergenic
ATGTCTTTAAGAAATGGGGT+TGG 0.618819 2.4:-53787311 MS.gene073435:CDS
ATATCATCAAAAGGGTACCA+TGG 0.628317 2.4:-53787899 MS.gene073435:CDS
GTATTGCAGGAAAGAATCCA+TGG 0.640699 2.4:+53787882 None:intergenic
CAAAAGAAATTGCAAAACTG+AGG 0.646915 2.4:+53787110 None:intergenic
TGAGTGAAGCAATCTAGCAA+TGG 0.650793 2.4:+53787479 None:intergenic
AATGAAACTTGCAAATACAA+CGG 0.655557 2.4:-53786956 MS.gene073435:CDS
TAATTGCCATCGCCTTACGG+TGG 0.656944 2.4:-53787544 MS.gene073435:CDS
AAGGTTCTTTAAAACTGACA+AGG 0.658226 2.4:-53787610 MS.gene073435:CDS
GATATTGCGTATGTCTGAGT+CGG 0.676717 2.4:+53787917 None:intergenic
ATGTCGAATTAAGCAAACAA+CGG 0.712898 2.4:+53787584 None:intergenic
GAGGGTTACCTCTAACCATG+CGG 0.732169 2.4:+53787793 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAACTATAACTAAACATATT+TGG + chr2.4:53787905-53787924 None:intergenic 15.0%
!! TAGTTATAGTTTACATAAAA+GGG - chr2.4:53787913-53787932 MS.gene073435:CDS 15.0%
!! TTAGTTATAGTTTACATAAA+AGG - chr2.4:53787912-53787931 MS.gene073435:CDS 15.0%
!! TTCAATTGATAAGATTAATT+TGG - chr2.4:53787823-53787842 MS.gene073435:CDS 15.0%
!!! TTTGATTATTTTTTATCCAA+AGG - chr2.4:53787326-53787345 MS.gene073435:CDS 15.0%
!! AGAGTAAAAATTGAAACTTT+GGG + chr2.4:53787217-53787236 None:intergenic 20.0%
!! AGGTATTAGTAAAAAACTTT+GGG - chr2.4:53787712-53787731 MS.gene073435:CDS 20.0%
!! TTCTTAACAATTCAAAAAGA+GGG + chr2.4:53787430-53787449 None:intergenic 20.0%
!!! AACTGATTTGATTAAATCAT+TGG - chr2.4:53787622-53787641 MS.gene073435:CDS 20.0%
!!! AAGGTATTAGTAAAAAACTT+TGG - chr2.4:53787711-53787730 MS.gene073435:CDS 20.0%
!!! AATTTGATGTCTTTAAGAAA+TGG - chr2.4:53787741-53787760 MS.gene073435:CDS 20.0%
!!! ATTTGATGTCTTTAAGAAAT+GGG - chr2.4:53787742-53787761 MS.gene073435:CDS 20.0%
!!! TTTTTTACTAATACCTTTCT+TGG + chr2.4:53787708-53787727 None:intergenic 20.0%
! AAGCTAATGTGTAAAATTGT+AGG + chr2.4:53787392-53787411 None:intergenic 25.0%
! AATGAAACTTGCAAATACAA+CGG - chr2.4:53788102-53788121 MS.gene073435:CDS 25.0%
! AGCTAATGTGTAAAATTGTA+GGG + chr2.4:53787391-53787410 None:intergenic 25.0%
! ATTGAAGTCAACATCAAATT+AGG + chr2.4:53787809-53787828 None:intergenic 25.0%
! CTTCTTAACAATTCAAAAAG+AGG + chr2.4:53787431-53787450 None:intergenic 25.0%
! GAGAGTAAAAATTGAAACTT+TGG + chr2.4:53787218-53787237 None:intergenic 25.0%
! TCAAAATCTTATACATTTGG+TGG + chr2.4:53788146-53788165 None:intergenic 25.0%
! TGATTTGATTAAATCATTGG+AGG - chr2.4:53787625-53787644 MS.gene073435:CDS 25.0%
!! CATTTACTTATTCTGAGAAT+CGG - chr2.4:53788011-53788030 MS.gene073435:CDS 25.0%
!! CTCTTTTTGAATTGTTAAGA+AGG - chr2.4:53787429-53787448 MS.gene073435:CDS 25.0%
!! TACAATTTTACACATTAGCT+TGG - chr2.4:53787391-53787410 MS.gene073435:CDS 25.0%
!! TTGCAATTTCTTTTGATGAA+AGG - chr2.4:53787956-53787975 MS.gene073435:CDS 25.0%
!! TTTCAATTTTTACTCTCCAA+AGG - chr2.4:53787221-53787240 MS.gene073435:CDS 25.0%
!! TTTGATGTCTTTAAGAAATG+GGG - chr2.4:53787743-53787762 MS.gene073435:CDS 25.0%
!!! TAATTTGGTGATGAGATTTT+GGG - chr2.4:53787838-53787857 MS.gene073435:CDS 25.0%
!!! TTAATTTGGTGATGAGATTT+TGG - chr2.4:53787837-53787856 MS.gene073435:CDS 25.0%
AAACCGTTATTCGAGTATTT+AGG - chr2.4:53787777-53787796 MS.gene073435:CDS 30.0%
ACATACGCAATATCATCAAA+AGG - chr2.4:53787150-53787169 MS.gene073435:CDS 30.0%
AGAATTCAAATTTGTCGATG+AGG + chr2.4:53787025-53787044 None:intergenic 30.0%
AGACCTAAATACTCGAATAA+CGG + chr2.4:53787783-53787802 None:intergenic 30.0%
ATGTCGAATTAAGCAAACAA+CGG + chr2.4:53787477-53787496 None:intergenic 30.0%
CAAAAGAAATTGCAAAACTG+AGG + chr2.4:53787951-53787970 None:intergenic 30.0%
CATACGCAATATCATCAAAA+GGG - chr2.4:53787151-53787170 MS.gene073435:CDS 30.0%
CGATTCTCAGAATAAGTAAA+TGG + chr2.4:53788013-53788032 None:intergenic 30.0%
TCAGTTGAACCAAATATAGA+TGG + chr2.4:53787608-53787627 None:intergenic 30.0%
!! AAGGTTCTTTAAAACTGACA+AGG - chr2.4:53787448-53787467 MS.gene073435:CDS 30.0%
!! AGATTTTGGGTCAATCATTT+AGG - chr2.4:53787851-53787870 MS.gene073435:CDS 30.0%
!! ATCAGTTTGATGACTGATTT+TGG - chr2.4:53787542-53787561 MS.gene073435:CDS 30.0%
!! ATCCTTCATTGACTACTTTT+GGG - chr2.4:53787669-53787688 MS.gene073435:CDS 30.0%
!!! GGTTTTGAAATAAAGCTCTT+TGG + chr2.4:53787072-53787091 None:intergenic 30.0%
AAGAAGTTGAGAACTGAATC+AGG + chr2.4:53787104-53787123 None:intergenic 35.0%
AAGCTTAATTCCAAGAACTG+AGG + chr2.4:53787287-53787306 None:intergenic 35.0%
ACATAAAAGGGTCATTCCAA+GGG - chr2.4:53787925-53787944 MS.gene073435:CDS 35.0%
AGCTTAATTCCAAGAACTGA+GGG + chr2.4:53787286-53787305 None:intergenic 35.0%
CAAATACAACGGAGAACAAT+GGG - chr2.4:53788113-53788132 MS.gene073435:CDS 35.0%
TACATAAAAGGGTCATTCCA+AGG - chr2.4:53787924-53787943 MS.gene073435:CDS 35.0%
TACTCGAATAACGGTTTCAT+CGG + chr2.4:53787774-53787793 None:intergenic 35.0%
TGGAGGAAGTAAAGAGTTTA+GGG - chr2.4:53787642-53787661 MS.gene073435:CDS 35.0%
TTCAAATTTGTCGATGAGGT+AGG + chr2.4:53787021-53787040 None:intergenic 35.0%
TTGGAGGAAGTAAAGAGTTT+AGG - chr2.4:53787641-53787660 MS.gene073435:CDS 35.0%
TTTGGGTCAATCATTTAGGT+TGG - chr2.4:53787855-53787874 MS.gene073435:CDS 35.0%
! AACTGAATCAGGTTTTTGTG+AGG + chr2.4:53787093-53787112 None:intergenic 35.0%
! ATGTCTTTAAGAAATGGGGT+TGG - chr2.4:53787747-53787766 MS.gene073435:CDS 35.0%
! CAAGTGTGTTTTCAGCTTTA+AGG - chr2.4:53788042-53788061 MS.gene073435:CDS 35.0%
! GTAGGGTTTGAAGTTAACAA+TGG + chr2.4:53787374-53787393 None:intergenic 35.0%
! TTGGGTCAATCATTTAGGTT+GGG - chr2.4:53787856-53787875 MS.gene073435:CDS 35.0%
!! ATATCATCAAAAGGGTACCA+TGG - chr2.4:53787159-53787178 MS.gene073435:CDS 35.0%
!! GATCCTTCATTGACTACTTT+TGG - chr2.4:53787668-53787687 MS.gene073435:CDS 35.0%
!! TCCTTCATTGACTACTTTTG+GGG - chr2.4:53787670-53787689 MS.gene073435:CDS 35.0%
!! TTTCAGCTTTAAGGAGGAAT+CGG - chr2.4:53788051-53788070 MS.gene073435:CDS 35.0%
!! TTTTAATTGCCATCGCCTTA+CGG - chr2.4:53787511-53787530 MS.gene073435:CDS 35.0%
AACTGATATTAGCCACCGTA+AGG + chr2.4:53787529-53787548 None:intergenic 40.0%
ATGAGGTAGGATACGGTAAA+TGG + chr2.4:53787008-53787027 None:intergenic 40.0%
CACTCAAGGCCATCTATATT+TGG - chr2.4:53787596-53787615 MS.gene073435:CDS 40.0%
GATATTGCGTATGTCTGAGT+CGG + chr2.4:53787144-53787163 None:intergenic 40.0%
GCAAATACAACGGAGAACAA+TGG - chr2.4:53788112-53788131 MS.gene073435:CDS 40.0%
GTATTGCAGGAAAGAATCCA+TGG + chr2.4:53787179-53787198 None:intergenic 40.0%
TGAGGTAGGATACGGTAAAT+GGG + chr2.4:53787007-53787026 None:intergenic 40.0%
TGAGTGAAGCAATCTAGCAA+TGG + chr2.4:53787582-53787601 None:intergenic 40.0%
TGGAAATTTAGCAAGTGCCA+AGG + chr2.4:53787885-53787904 None:intergenic 40.0%
TTGCTAGATTGCTTCACTCA+AGG - chr2.4:53787582-53787601 MS.gene073435:CDS 40.0%
TTTGTCGATGAGGTAGGATA+CGG + chr2.4:53787015-53787034 None:intergenic 40.0%
! TTTCTCGGTCGTAATGCTTT+TGG - chr2.4:53786927-53786946 MS.gene073435:CDS 40.0%
!! AATCGTTTTGTGGGTATTGC+AGG + chr2.4:53787192-53787211 None:intergenic 40.0%
!! ACTTTGGGAGAATCGTTTTG+TGG + chr2.4:53787202-53787221 None:intergenic 40.0%
!! ATCAGATGAAGAAGCACCTT+TGG + chr2.4:53787345-53787364 None:intergenic 40.0%
!! CTTTGGGAGAATCGTTTTGT+GGG + chr2.4:53787201-53787220 None:intergenic 40.0%
!! TCATTTAGGTTGGGATTCCT+TGG - chr2.4:53787865-53787884 MS.gene073435:CDS 40.0%
!!! GTGTGTTTTCAGCTTTAAGG+AGG - chr2.4:53788045-53788064 MS.gene073435:CDS 40.0%
AATTGCAAAACTGAGGCCCT+TGG + chr2.4:53787944-53787963 None:intergenic 45.0%
GCCCCAAAAGTAGTCAATGA+AGG + chr2.4:53787674-53787693 None:intergenic 45.0%
TACGGTAAATGGGTGTGAAG+AGG + chr2.4:53786997-53787016 None:intergenic 45.0%
TAGAGGTAACCCTCAGTTCT+TGG - chr2.4:53787274-53787293 MS.gene073435:CDS 45.0%
TGAATCTGACATTGTCCGCA+TGG - chr2.4:53787250-53787269 MS.gene073435:CDS 45.0%
!! CTCTTTGGAAGCTTTGAGTG+CGG + chr2.4:53787057-53787076 None:intergenic 45.0%
!! GCTGCTTTGAAAGCCAAGAA+AGG - chr2.4:53787692-53787711 MS.gene073435:CDS 45.0%
!! GTCAGATTCAGAAGCACCTT+TGG + chr2.4:53787240-53787259 None:intergenic 45.0%
GACATTGTCCGCATGGTTAG+AGG - chr2.4:53787257-53787276 MS.gene073435:CDS 50.0%
GAGGGTTACCTCTAACCATG+CGG + chr2.4:53787268-53787287 None:intergenic 50.0%
GGTAAATGGGTGTGAAGAGG+TGG + chr2.4:53786994-53787013 None:intergenic 50.0%
TAATTGCCATCGCCTTACGG+TGG - chr2.4:53787514-53787533 MS.gene073435:CDS 50.0%
TATTAGCCACCGTAAGGCGA+TGG + chr2.4:53787523-53787542 None:intergenic 50.0%
!! GAAGCTTTGAGTGCGGATTC+AGG + chr2.4:53787050-53787069 None:intergenic 50.0%
!! GTGAAGAGGTGGTAGTGGTA+AGG + chr2.4:53786983-53787002 None:intergenic 50.0%
TGGGTGTGAAGAGGTGGTAG+TGG + chr2.4:53786988-53787007 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr2.4 gene 53786915 53788165 53786915 ID=MS.gene073435
chr2.4 mRNA 53786915 53788165 53786915 ID=MS.gene073435.t1;Parent=MS.gene073435
chr2.4 exon 53786915 53788165 53786915 ID=MS.gene073435.t1.exon1;Parent=MS.gene073435.t1
chr2.4 CDS 53786915 53788165 53786915 ID=cds.MS.gene073435.t1;Parent=MS.gene073435.t1
Gene Sequence

>MS.gene073435

ATGTTGAAAGCATTTCTCGGTCGTAATGCTTTTGGCATATCAAATTACTCACAAACACTCAGATTCCTTACCACTACCACCTCTTCACACCCATTTACCGTATCCTACCTCATCGACAAATTTGAATTCTCACCTGAATCCGCACTCAAAGCTTCCAAAGAGCTTTATTTCAAAACCTCACAAAAACCTGATTCAGTTCTCAACTTCTTCAAAAATCACAGTTTCACCGACTCAGACATACGCAATATCATCAAAAGGGTACCATGGATTCTTTCCTGCAATACCCACAAAACGATTCTCCCAAAGTTTCAATTTTTACTCTCCAAAGGTGCTTCTGAATCTGACATTGTCCGCATGGTTAGAGGTAACCCTCAGTTCTTGGAATTAAGCTTGAAGAATCATCAAATTAAGTTTGATTATTTTTTATCCAAAGGTGCTTCTTCATCTGATATTGTTCCATTGTTAACTTCAAACCCTACAATTTTACACATTAGCTTGGAGAAACGAATAATCCCTCTTTTTGAATTGTTAAGAAGGTTCTTTAAAACTGACAAGGATACCGTTGTTTGCTTAATTCGACATTCAACTACATTTACTTTTAATTGCCATCGCCTTACGGTGGCTAATATCAGTTTGATGACTGATTTTGGAGTTTCTGATTCTACCATTGCTAGATTGCTTCACTCAAGGCCATCTATATTTGGTTCAACTGATTTGATTAAATCATTGGAGGAAGTAAAGAGTTTAGGGTTTGATCCTTCATTGACTACTTTTGGGGCTGCTTTGAAAGCCAAGAAAGGTATTAGTAAAAAACTTTGGGATGAGAAATTTGATGTCTTTAAGAAATGGGGTTGGTCCGATGAAACCGTTATTCGAGTATTTAGGTCTCAGCCTAATTTGATGTTGACTTCAATTGATAAGATTAATTTGGTGATGAGATTTTGGGTCAATCATTTAGGTTGGGATTCCTTGGCACTTGCTAAATTTCCAAATATGTTTAGTTATAGTTTACATAAAAGGGTCATTCCAAGGGCCTCAGTTTTGCAATTTCTTTTGATGAAAGGTTTGCGAAAAAAGAATGCAAGCTTAGTCACACCATTTACTTATTCTGAGAATCGGTTCTTGAACAAGTGTGTTTTCAGCTTTAAGGAGGAATCGGATTATCTGTTGAAGCTATATGACGAAAAAATGAAACTTGCAAATACAACGGAGAACAATGGGATGTCATCCACCAAATGTATAAGATTTTGA
Protein sequence

>MS.gene073435.t1

MLKAFLGRNAFGISNYSQTLRFLTTTTSSHPFTVSYLIDKFEFSPESALKASKELYFKTSQKPDSVLNFFKNHSFTDSDIRNIIKRVPWILSCNTHKTILPKFQFLLSKGASESDIVRMVRGNPQFLELSLKNHQIKFDYFLSKGASSSDIVPLLTSNPTILHISLEKRIIPLFELLRRFFKTDKDTVVCLIRHSTTFTFNCHRLTVANISLMTDFGVSDSTIARLLHSRPSIFGSTDLIKSLEEVKSLGFDPSLTTFGAALKAKKGISKKLWDEKFDVFKKWGWSDETVIRVFRSQPNLMLTSIDKINLVMRFWVNHLGWDSLALAKFPNMFSYSLHKRVIPRASVLQFLLMKGLRKKNASLVTPFTYSENRFLNKCVFSFKEESDYLLKLYDEKMKLANTTENNGMSSTKCIRF