AlfalfaGEDB | MS.gene073441

M. sativa cultivar XinJiangDaYe / MS.gene073441

KEGG: -
GO: GO:0000976 GO:0000981 GO:0000987 GO:0001067 GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0003700 GO:0005488 GO:0005515 GO:0006355 GO:0006357 GO:0008150 GO:0009889 GO:0009891 GO:0009893 GO:0010468 GO:0010556 GO:0010557 GO:0010604 GO:0010628 GO:0019219 GO:0019222 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0035326 GO:0043565 GO:0044212 GO:0045893 GO:0045935 GO:0045944 GO:0046983 GO:0048518 GO:0048522 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902680 GO:1903506 GO:1903508 GO:1990837 GO:2000112 GO:2001141
PFAM: PF00319.21

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073441.t1	XP_003594864.1	87.3	158	20	0	1	158	1	158	9.70E-71	276.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073441.t1	Q9FJK3	39.6	154	93	0	1	154	1	154	6.9e-25	115.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073441.t1	G7IND2	87.3	158	20	0	1	158	1	158	7.0e-71	276.2

TFs/TRs:

Gene ID	Type	Classification
MS.gene073441.t1	TF	MADS-M-type

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073441.t1	MTR_2g035580	91.139	158	14	0	1	158	1	158	2.63e-93	267
MS.gene073441.t1	MTR_3g466980	46.203	158	85	0	1	158	1	158	2.70e-46	150
MS.gene073441.t1	MTR_3g467080	49.351	154	77	1	1	153	1	154	5.89e-46	147
MS.gene073441.t1	MTR_4g063790	46.753	154	81	1	1	153	1	154	5.33e-43	140
MS.gene073441.t1	MTR_3g065100	44.156	154	86	0	1	154	1	154	5.25e-38	130
MS.gene073441.t1	MTR_3g031100	42.138	159	91	1	1	158	1	159	7.59e-37	127
MS.gene073441.t1	MTR_4g032620	37.662	154	95	1	1	153	1	154	8.58e-35	121
MS.gene073441.t1	MTR_3g465410	47.541	122	63	1	30	150	2	123	1.36e-33	115
MS.gene073441.t1	MTR_2g016210	38.750	160	93	2	1	157	1	158	6.85e-33	117
MS.gene073441.t1	MTR_3g466830	39.597	149	90	0	4	152	3	151	8.01e-33	114
MS.gene073441.t1	MTR_3g031240	38.462	156	95	1	1	155	1	156	5.24e-31	110
MS.gene073441.t1	MTR_5g075380	33.333	153	102	0	1	153	1	153	1.46e-30	108
MS.gene073441.t1	MTR_4g031910	37.179	156	97	1	1	155	1	156	2.69e-29	107
MS.gene073441.t1	MTR_8g036130	37.975	158	95	2	1	155	1	158	3.05e-29	107
MS.gene073441.t1	MTR_1g090783	33.333	153	101	1	6	158	5	156	1.79e-28	103
MS.gene073441.t1	MTR_5g047580	34.194	155	101	1	1	155	1	154	4.95e-28	102
MS.gene073441.t1	MTR_4g032260	36.943	157	98	1	1	156	1	157	2.64e-27	100
MS.gene073441.t1	MTR_1g077390	33.987	153	100	1	1	153	1	152	5.48e-27	99.4
MS.gene073441.t1	MTR_3g466890	35.417	144	93	0	6	149	5	148	2.82e-26	97.8
MS.gene073441.t1	MTR_1g090697	30.968	155	106	1	1	155	1	154	1.18e-23	90.9
MS.gene073441.t1	MTR_2g035610	36.752	117	74	0	1	117	1	117	2.34e-23	89.0
MS.gene073441.t1	MTR_4g032290	35.032	157	101	1	1	156	1	157	1.08e-22	90.1
MS.gene073441.t1	MTR_4g028720	35.897	156	97	3	1	155	1	154	5.85e-22	86.7
MS.gene073441.t1	MTR_3g466930	36.885	122	77	0	32	153	2	123	1.05e-21	85.1
MS.gene073441.t1	MTR_1g090710	31.210	157	104	3	1	155	1	155	1.18e-21	85.9
MS.gene073441.t1	MTR_7g011950	32.903	155	103	1	1	155	1	154	1.60e-21	85.5
MS.gene073441.t1	MTR_1g084950	34.194	155	101	1	1	155	1	154	2.90e-21	84.7
MS.gene073441.t1	MTR_3g466900	35.246	122	79	0	32	153	2	123	1.01e-20	82.8
MS.gene073441.t1	MTR_1g077320	30.323	155	107	1	1	155	1	154	6.20e-20	81.3
MS.gene073441.t1	MTR_1g077300	36.129	155	92	4	1	153	1	150	5.37e-18	76.3
MS.gene073441.t1	MTR_4g028800	41.096	73	41	1	1	73	1	71	7.90e-18	73.6
MS.gene073441.t1	MTR_5g047560	25.806	155	98	2	1	155	1	138	1.13e-13	64.7
MS.gene073441.t1	MTR_4g019670	28.814	118	80	1	11	128	14	127	5.26e-11	60.1
MS.gene073441.t1	MTR_7g106510	27.273	121	78	2	11	128	14	127	6.76e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073441.t1	AT5G48670	41.667	156	87	2	1	154	1	154	6.84e-32	116
MS.gene073441.t1	AT3G05860	36.913	149	94	0	1	149	1	149	2.48e-29	107
MS.gene073441.t1	AT1G65300	37.086	151	95	0	4	154	3	153	3.03e-29	108
MS.gene073441.t1	AT1G65330	39.333	150	89	2	6	154	5	153	3.43e-29	108
MS.gene073441.t1	AT3G05860	39.103	156	81	3	1	149	1	149	1.32e-28	106
MS.gene073441.t1	AT3G05860	39.103	156	81	3	1	149	1	149	1.47e-28	106
MS.gene073441.t1	AT1G31630	33.333	153	91	3	6	153	5	151	6.79e-24	95.5
MS.gene073441.t1	AT1G31640	35.915	142	90	1	11	152	10	150	1.98e-23	95.5
MS.gene073441.t1	AT5G27810	43.182	88	50	0	30	117	2	89	7.43e-23	87.8
MS.gene073441.t1	AT5G26650	32.237	152	103	0	4	155	2	153	1.68e-22	92.0
MS.gene073441.t1	AT5G27960	31.579	152	104	0	4	155	2	153	1.35e-21	89.0
MS.gene073441.t1	AT1G22590	36.111	108	69	0	11	118	11	118	5.07e-21	84.3
MS.gene073441.t1	AT2G28700	32.680	153	95	3	1	149	1	149	5.85e-20	84.7
MS.gene073441.t1	AT2G40210	32.479	117	79	0	1	117	1	117	9.34e-15	70.5
MS.gene073441.t1	AT5G26580	28.571	154	96	3	1	154	1	140	2.89e-14	69.3
MS.gene073441.t1	AT5G26630	26.623	154	113	0	1	154	1	154	3.26e-14	67.8
MS.gene073441.t1	AT5G06500	28.182	110	75	1	1	110	1	106	4.28e-13	65.1

Find 26 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GAAGATCTATCATTGCTTAT+TGG	0.201999	2.4:-53714040	MS.gene073441:CDS
CTTCTCTTTCGATTTCATTA+TGG	0.221424	2.4:+53714168	None:intergenic
ATTCATACTGGTGAATTTGA+TGG	0.332075	2.4:-53714085	MS.gene073441:CDS
AGATGGTGTTGGACAAGTTT+AGG	0.350542	2.4:-53714252	MS.gene073441:CDS
CTTGGTGAATTCATTCATAC+TGG	0.375906	2.4:-53714097	MS.gene073441:CDS
GTTTAGGAGCTTTCCTGAAT+CGG	0.417309	2.4:-53714236	MS.gene073441:CDS
TAAACTTGTCCAACACCATC+TGG	0.418271	2.4:+53714254	None:intergenic
AGTGAAATCACCACACTTTG+TGG	0.451943	2.4:-53714349	MS.gene073441:CDS
GGAAGACAACATTCAACAAA+AGG	0.464060	2.4:-53714780	MS.gene073441:CDS
ATCTATGATGAAAACAATGA+TGG	0.489972	2.4:-53714307	MS.gene073441:CDS
AGAGAAGTTGAAGAAACAAA+AGG	0.500916	2.4:-53714152	MS.gene073441:CDS
CCTGAATCGGAACAAAGAAA+AGG	0.516491	2.4:-53714223	MS.gene073441:CDS
GGAACAAAGAAAAGGAAAGC+TGG	0.520124	2.4:-53714215	MS.gene073441:CDS
AGTTGAAGAAACAAAAGGAC+AGG	0.532305	2.4:-53714147	MS.gene073441:CDS
AAAAGGAAAGCTGGATCATG+AGG	0.532487	2.4:-53714206	MS.gene073441:CDS
AAGAAAATGAATGATCTACT+TGG	0.551967	2.4:-53714115	MS.gene073441:CDS
ACGGTTTATCCATCAAAAGA+AGG	0.566324	2.4:-53714280	MS.gene073441:CDS
ACATAACTGATGACTTGAAG+AGG	0.573872	2.4:-53714801	MS.gene073441:CDS
AGAAGGAGTCCAGATGGTGT+TGG	0.590630	2.4:-53714263	MS.gene073441:CDS
ACCGAAAAGAAGTCGATGAG+AGG	0.615793	2.4:-53714012	MS.gene073441:CDS
ATCAAAAGAAGGAGTCCAGA+TGG	0.615817	2.4:-53714269	MS.gene073441:CDS
TGCATCAATTCCACAAAGTG+TGG	0.616904	2.4:+53714339	None:intergenic
TGAAAACAATGATGGAGCAA+CGG	0.627126	2.4:-53714299	MS.gene073441:CDS
ACATTCAACAAAAGGAAGCA+TGG	0.630577	2.4:-53714772	MS.gene073441:intron
AAAGGAAAGCTGGATCATGA+GGG	0.661580	2.4:-53714205	MS.gene073441:CDS
CTTTGTGGAATTGATGCATG+TGG	0.684212	2.4:-53714334	MS.gene073441:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTGTTCTTTATAGAAATAA+AGG	+	chr2.4:53714091-53714110	None:intergenic	20.0%
!!	GAAAAAATTTATACTATAGC+TGG	+	chr2.4:53714382-53714401	None:intergenic	20.0%
!!	GAAAATAACGATAATTTCAA+AGG	+	chr2.4:53714340-53714359	None:intergenic	20.0%
!!	TCATAACTGATATGTTATTA+TGG	+	chr2.4:53714317-53714336	None:intergenic	20.0%
!!	TCATATTGATAAATTCCTAA+TGG	+	chr2.4:53714217-53714236	None:intergenic	20.0%
!!!	TTTCATATTTTTAGTATCCA+TGG	+	chr2.4:53714181-53714200	None:intergenic	20.0%
!	AAGAAAATGAATGATCTACT+TGG	-	chr2.4:53714705-53714724	MS.gene073441:intron	25.0%
!	ATCTATGATGAAAACAATGA+TGG	-	chr2.4:53714513-53714532	MS.gene073441:intron	25.0%
!!	ATCATTCATTTTCTTCTTCT+TGG	+	chr2.4:53714700-53714719	None:intergenic	25.0%
!!!	GTTTTTTTTTATCACTACCA+TGG	-	chr2.4:53714161-53714180	MS.gene073441:CDS	25.0%
	AGAGAAGTTGAAGAAACAAA+AGG	-	chr2.4:53714668-53714687	MS.gene073441:intron	30.0%
	ATTCATACTGGTGAATTTGA+TGG	-	chr2.4:53714735-53714754	MS.gene073441:intron	30.0%
	CTTCTCTTTCGATTTCATTA+TGG	+	chr2.4:53714655-53714674	None:intergenic	30.0%
	GAAGATCTATCATTGCTTAT+TGG	-	chr2.4:53714780-53714799	MS.gene073441:CDS	30.0%
!!	GTTTTGTTGTGTTTTTCAAC+AGG	-	chr2.4:53714432-53714451	MS.gene073441:intron	30.0%
	AAGTTGACTGCATAACCATT+AGG	-	chr2.4:53714199-53714218	MS.gene073441:CDS	35.0%
	ACATAACTGATGACTTGAAG+AGG	-	chr2.4:53714019-53714038	MS.gene073441:CDS	35.0%
	ACATTCAACAAAAGGAAGCA+TGG	-	chr2.4:53714048-53714067	MS.gene073441:CDS	35.0%
	ACGGTTTATCCATCAAAAGA+AGG	-	chr2.4:53714540-53714559	MS.gene073441:intron	35.0%
	AGTTGAAGAAACAAAAGGAC+AGG	-	chr2.4:53714673-53714692	MS.gene073441:intron	35.0%
	CTTGGTGAATTCATTCATAC+TGG	-	chr2.4:53714723-53714742	MS.gene073441:intron	35.0%
	GGAAGACAACATTCAACAAA+AGG	-	chr2.4:53714040-53714059	MS.gene073441:CDS	35.0%
!	TGAAAACAATGATGGAGCAA+CGG	-	chr2.4:53714521-53714540	MS.gene073441:intron	35.0%
	AAAAGGAAAGCTGGATCATG+AGG	-	chr2.4:53714614-53714633	MS.gene073441:intron	40.0%
	AAAGGAAAGCTGGATCATGA+GGG	-	chr2.4:53714615-53714634	MS.gene073441:intron	40.0%
	AGTGAAATCACCACACTTTG+TGG	-	chr2.4:53714471-53714490	MS.gene073441:intron	40.0%
	ATCAAAAGAAGGAGTCCAGA+TGG	-	chr2.4:53714551-53714570	MS.gene073441:intron	40.0%
	CCTGAATCGGAACAAAGAAA+AGG	-	chr2.4:53714597-53714616	MS.gene073441:intron	40.0%
	CTTTGTGGAATTGATGCATG+TGG	-	chr2.4:53714486-53714505	MS.gene073441:intron	40.0%
	GGAACAAAGAAAAGGAAAGC+TGG	-	chr2.4:53714605-53714624	MS.gene073441:intron	40.0%
	GTTTAGGAGCTTTCCTGAAT+CGG	-	chr2.4:53714584-53714603	MS.gene073441:intron	40.0%
	TAAACTTGTCCAACACCATC+TGG	+	chr2.4:53714569-53714588	None:intergenic	40.0%
	TGCATCAATTCCACAAAGTG+TGG	+	chr2.4:53714484-53714503	None:intergenic	40.0%
!	AGATGGTGTTGGACAAGTTT+AGG	-	chr2.4:53714568-53714587	MS.gene073441:intron	40.0%
!	ATCTGGACTCCTTCTTTTGA+TGG	+	chr2.4:53714552-53714571	None:intergenic	40.0%
!	CCTTTTCTTTGTTCCGATTC+AGG	+	chr2.4:53714600-53714619	None:intergenic	40.0%
!!!	TCCTCTCATCGACTTCTTTT+CGG	+	chr2.4:53714812-53714831	None:intergenic	40.0%
	ACCGAAAAGAAGTCGATGAG+AGG	-	chr2.4:53714808-53714827	MS.gene073441:CDS	45.0%
	ATTTCCCTCAACTCGCTCAT+GGG	-	chr2.4:53714410-53714429	MS.gene073441:intron	45.0%
!	AAAACCCATGAGCGAGTTGA+GGG	+	chr2.4:53714417-53714436	None:intergenic	45.0%
	AGAAGGAGTCCAGATGGTGT+TGG	-	chr2.4:53714557-53714576	MS.gene073441:intron	50.0%
	CAAAACCCATGAGCGAGTTG+AGG	+	chr2.4:53714418-53714437	None:intergenic	50.0%
	CATTTCCCTCAACTCGCTCA+TGG	-	chr2.4:53714409-53714428	MS.gene073441:intron	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.4	gene	53713991	53714851	53713991	ID=MS.gene073441
chr2.4	mRNA	53713991	53714851	53713991	ID=MS.gene073441.t1;Parent=MS.gene073441
chr2.4	exon	53714773	53714851	53714773	ID=MS.gene073441.t1.exon1;Parent=MS.gene073441.t1
chr2.4	CDS	53714773	53714851	53714773	ID=cds.MS.gene073441.t1;Parent=MS.gene073441.t1
chr2.4	exon	53713991	53714388	53713991	ID=MS.gene073441.t1.exon2;Parent=MS.gene073441.t1
chr2.4	CDS	53713991	53714388	53713991	ID=cds.MS.gene073441.t1;Parent=MS.gene073441.t1

Gene Sequence

>MS.gene073441

ATGGCTGATAAAAATATGAGAGTTGACTACATAACTGATGACTTGAAGAGGAAGACAACATTCAACAAAAGGAAGCATGGTTAGATACTAAATTTCTCCTTTATTTCTATAAAGAACACTCATATCATACAAATTTATTATTATGAATTTTTTCTTGTTCATTTCTTTCAGTTTTTTTTTATCACTACCATGGATACTAAAAATATGAAAGTTGACTGCATAACCATTAGGAATTTATCAATATGAATATAGTTATTTGATGTTTGTTATTTTTTTTGTTGTTGTTGATATCATGAAAAAATTGTAAGCAAATGTTGTTGCAACCATAATAACATATCAGTTATGACCTTTGAAATTATCGTTATTTTCACAAAATTTTAAGATCATGCCAGCTATAGTATAAATTTTTTCAGTTTCACATTTCCCTCAACTCGCTCATGGGTTTTGTTGTGTTTTTCAACAGGTATAACTAAGAAGATTAGTGAAATCACCACACTTTGTGGAATTGATGCATGTGGTATAATCTATGATGAAAACAATGATGGAGCAACGGTTTATCCATCAAAAGAAGGAGTCCAGATGGTGTTGGACAAGTTTAGGAGCTTTCCTGAATCGGAACAAAGAAAAGGAAAGCTGGATCATGAGGGCTTTTTGAGACAATCCATAATGAAATCGAAAGAGAAGTTGAAGAAACAAAAGGACAGGTCCAAGAAGAAGAAAATGAATGATCTACTTGGTGAATTCATTCATACTGGTGAATTTGATGGAAATGTGAGCGAGAATGATCTCGAAGATCTATCATTGCTTATTGGTGATTACCGAAAAGAAGTCGATGAGAGGATCGAATCAAGTCAAACCTGA

Protein sequence

>MS.gene073441.t1

MADKNMRVDYITDDLKRKTTFNKRKHGITKKISEITTLCGIDACGIIYDENNDGATVYPSKEGVQMVLDKFRSFPESEQRKGKLDHEGFLRQSIMKSKEKLKKQKDRSKKKKMNDLLGEFIHTGEFDGNVSENDLEDLSLLIGDYRKEVDERIESSQT