Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073645.t1 | RHN78231.1 | 89 | 154 | 17 | 0 | 14 | 167 | 144 | 297 | 4.20E-69 | 271.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073645.t1 | A0A396JNG9 | 89.0 | 154 | 17 | 0 | 14 | 167 | 144 | 297 | 3.0e-69 | 271.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene073645.t1 | TF | B3 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene073645.t1 | MTR_1g035640 | 52.000 | 200 | 87 | 4 | 8 | 206 | 139 | 330 | 3.61e-55 | 182 |
| MS.gene073645.t1 | MTR_1g035620 | 50.746 | 201 | 61 | 10 | 5 | 199 | 141 | 309 | 4.88e-45 | 154 |
| MS.gene073645.t1 | MTR_1g035490 | 44.388 | 196 | 82 | 4 | 6 | 199 | 61 | 231 | 6.04e-44 | 150 |
| MS.gene073645.t1 | MTR_1g035570 | 45.361 | 194 | 93 | 4 | 20 | 211 | 156 | 338 | 1.50e-41 | 145 |
| MS.gene073645.t1 | MTR_1g050845 | 51.724 | 203 | 64 | 8 | 5 | 201 | 128 | 302 | 2.80e-41 | 149 |
| MS.gene073645.t1 | MTR_1g035650 | 89.855 | 69 | 7 | 0 | 5 | 73 | 135 | 203 | 2.44e-37 | 129 |
| MS.gene073645.t1 | MTR_6g061070 | 40.777 | 206 | 103 | 6 | 9 | 200 | 171 | 371 | 7.35e-36 | 131 |
| MS.gene073645.t1 | MTR_5g044670 | 31.875 | 160 | 94 | 5 | 20 | 167 | 161 | 317 | 7.62e-18 | 80.5 |
| MS.gene073645.t1 | MTR_1g035540 | 57.447 | 47 | 20 | 0 | 118 | 164 | 31 | 77 | 2.02e-12 | 62.4 |
| MS.gene073645.t1 | MTR_8g103940 | 35.433 | 127 | 72 | 5 | 47 | 173 | 232 | 348 | 1.30e-11 | 63.5 |
| MS.gene073645.t1 | MTR_1g108780 | 31.333 | 150 | 81 | 6 | 32 | 167 | 163 | 304 | 3.51e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 28 sgRNAs with CRISPR-Local
Find 86 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTACGCCAAAGGTCTTTATT+TGG | 0.224792 | 1.1:+35168707 | MS.gene073645:CDS |
| TGACATTTCAACTTCAAAAC+TGG | 0.262282 | 1.1:-35168679 | None:intergenic |
| TTAAAGGAACTTCCAGTTCT+AGG | 0.280821 | 1.1:+35169547 | MS.gene073645:CDS |
| GCTAAGAGCAAAGACATTAA+AGG | 0.303545 | 1.1:+35169531 | MS.gene073645:CDS |
| AGCAAGAAATTATCATCCTT+TGG | 0.369605 | 1.1:+35168650 | MS.gene073645:CDS |
| ATTTATAGTTGGTGAAGATA+TGG | 0.400385 | 1.1:+35169176 | MS.gene073645:CDS |
| ACATACCAAATAAAGACCTT+TGG | 0.466172 | 1.1:-35168712 | None:intergenic |
| CCAATGGAATTTATGAAAAG+AGG | 0.495389 | 1.1:+35169759 | MS.gene073645:CDS |
| GAGACAGCAAGATTTATAGT+TGG | 0.496722 | 1.1:+35169165 | MS.gene073645:CDS |
| GGTCACAGTAGATGATTTAG+TGG | 0.504279 | 1.1:-35169229 | None:intergenic |
| CCTTGACTAGGTTATAAAGA+AGG | 0.508003 | 1.1:+35169486 | MS.gene073645:intron |
| ATGATAATAGTGAATAAAAG+TGG | 0.508803 | 1.1:-35169330 | None:intergenic |
| TGTAACTTTGCAGGTAGTAA+TGG | 0.513785 | 1.1:+35168570 | MS.gene073645:intron |
| GACATTTCAACTTCAAAACT+GGG | 0.528604 | 1.1:-35168678 | None:intergenic |
| CATCTACTGTGACCACTGGT+TGG | 0.552617 | 1.1:+35169238 | MS.gene073645:CDS |
| AAATCATCTACTGTGACCAC+TGG | 0.561649 | 1.1:+35169234 | MS.gene073645:CDS |
| TGGAGGAGGTATGATTAAGA+AGG | 0.562815 | 1.1:+35168590 | MS.gene073645:CDS |
| CTTGACTAGGTTATAAAGAA+GGG | 0.564516 | 1.1:+35169487 | MS.gene073645:intron |
| TCTAAGAAGTTGAAAATGCA+AGG | 0.567006 | 1.1:+35169378 | MS.gene073645:CDS |
| TAATAGTGAATAAAAGTGGT+TGG | 0.584021 | 1.1:-35169326 | None:intergenic |
| TATCATTACTCGAGCAAGAG+AGG | 0.588412 | 1.1:+35169347 | MS.gene073645:CDS |
| GCTAAATGTCTTCCAACCAG+TGG | 0.593840 | 1.1:-35169250 | None:intergenic |
| ATATATACAGGATGTACCAA+TGG | 0.596542 | 1.1:+35169743 | MS.gene073645:intron |
| AACTTTGCAGGTAGTAATGG+AGG | 0.609546 | 1.1:+35168573 | MS.gene073645:intron |
| ATGTCACAATCTTACGCCAA+AGG | 0.623356 | 1.1:+35168696 | MS.gene073645:CDS |
| TTTGCAGGTAGTAATGGAGG+AGG | 0.632526 | 1.1:+35168576 | MS.gene073645:intron |
| TATTGAAACAGAGGATACCA+AGG | 0.654958 | 1.1:+35169109 | MS.gene073645:intron |
| ATTGAAACAGAGGATACCAA+GGG | 0.683744 | 1.1:+35169110 | MS.gene073645:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTAAACTTTTATATATAC+AGG | + | chr1.1:35169731-35169750 | MS.gene073645:intron | 10.0% |
| !! | TATAAAACAAAATCTAATGA+AGG | - | chr1.1:35169016-35169035 | None:intergenic | 15.0% |
| !!! | AAAAAATATAATTTGCAAGT+TGG | + | chr1.1:35169273-35169292 | MS.gene073645:CDS | 15.0% |
| !!! | ATATTTGGTTTCATAAAAAT+TGG | + | chr1.1:35168186-35168205 | MS.gene073645:intron | 15.0% |
| !!! | GTTCTAGTTTTTAAATATAA+AGG | + | chr1.1:35168242-35168261 | MS.gene073645:intron | 15.0% |
| !!! | TATTTGGTTTCATAAAAATT+GGG | + | chr1.1:35168187-35168206 | MS.gene073645:intron | 15.0% |
| !!! | TTTTATAAAAGTGTAACAAA+TGG | + | chr1.1:35169029-35169048 | MS.gene073645:intron | 15.0% |
| !! | AAAAATATTTATCTTGCAGA+GGG | + | chr1.1:35168055-35168074 | MS.gene073645:intron | 20.0% |
| !! | ATGATAATAGTGAATAAAAG+TGG | - | chr1.1:35169333-35169352 | None:intergenic | 20.0% |
| !! | GACAATGTTACTAATATTAA+TGG | + | chr1.1:35168365-35168384 | MS.gene073645:intron | 20.0% |
| !! | TAAGAGAAAATTGTCAAAAT+AGG | + | chr1.1:35168976-35168995 | MS.gene073645:intron | 20.0% |
| !! | TGTTTCAAAGTTGAAATATT+TGG | + | chr1.1:35168272-35168291 | MS.gene073645:intron | 20.0% |
| !!! | CTAGTTTTTAAATATAAAGG+AGG | + | chr1.1:35168245-35168264 | MS.gene073645:intron | 20.0% |
| !!! | TCTATCAAAATTCTCTATTT+TGG | + | chr1.1:35168737-35168756 | MS.gene073645:intron | 20.0% |
| !!! | TTTTATATATGTAACTTTGC+AGG | + | chr1.1:35168561-35168580 | MS.gene073645:intron | 20.0% |
| !!! | TTTTGAATAAGTATAGTTGT+TGG | - | chr1.1:35168039-35168058 | None:intergenic | 20.0% |
| ! | AATATTTGGTTGCAATACAT+TGG | + | chr1.1:35168286-35168305 | MS.gene073645:intron | 25.0% |
| ! | CAAAAATATTTATCTTGCAG+AGG | + | chr1.1:35168054-35168073 | MS.gene073645:intron | 25.0% |
| ! | TAATAGTGAATAAAAGTGGT+TGG | - | chr1.1:35169329-35169348 | None:intergenic | 25.0% |
| !! | ATTTTCAACTTCTTAGAGTT+AGG | - | chr1.1:35169375-35169394 | None:intergenic | 25.0% |
| !! | TGCTAAACAAGATGTATTTT+GGG | + | chr1.1:35168148-35168167 | MS.gene073645:intron | 25.0% |
| !! | TTCAGCTTCAACTTTTATAT+TGG | - | chr1.1:35168324-35168343 | None:intergenic | 25.0% |
| !!! | ATTTATAGTTGGTGAAGATA+TGG | + | chr1.1:35169176-35169195 | MS.gene073645:CDS | 25.0% |
| !!! | TTTGAACATTGTTTTTCCTT+TGG | + | chr1.1:35169652-35169671 | MS.gene073645:intron | 25.0% |
| AATTTATTTGACCCTTGACT+AGG | + | chr1.1:35169474-35169493 | MS.gene073645:intron | 30.0% | |
| ACAAACTGATATTGAAACAG+AGG | + | chr1.1:35169100-35169119 | MS.gene073645:intron | 30.0% | |
| ACATACCAAATAAAGACCTT+TGG | - | chr1.1:35168715-35168734 | None:intergenic | 30.0% | |
| AGCAAGAAATTATCATCCTT+TGG | + | chr1.1:35168650-35168669 | MS.gene073645:CDS | 30.0% | |
| ATATATACAGGATGTACCAA+TGG | + | chr1.1:35169743-35169762 | MS.gene073645:intron | 30.0% | |
| ATGTAGCTCCACAAAATATT+TGG | - | chr1.1:35168084-35168103 | None:intergenic | 30.0% | |
| CAAGGGTCAAATAAATTAAG+TGG | - | chr1.1:35169471-35169490 | None:intergenic | 30.0% | |
| CATACATCTCAAAGGAAAAA+AGG | + | chr1.1:35168396-35168415 | MS.gene073645:intron | 30.0% | |
| CCAATGGAATTTATGAAAAG+AGG | + | chr1.1:35169759-35169778 | MS.gene073645:CDS | 30.0% | |
| CTTCTTTATAACCTAGTCAA+GGG | - | chr1.1:35169488-35169507 | None:intergenic | 30.0% | |
| CTTGACTAGGTTATAAAGAA+GGG | + | chr1.1:35169487-35169506 | MS.gene073645:intron | 30.0% | |
| GACATTTCAACTTCAAAACT+GGG | - | chr1.1:35168681-35168700 | None:intergenic | 30.0% | |
| GAGAGAATACTTGAAAAAGT+TGG | + | chr1.1:35169140-35169159 | MS.gene073645:CDS | 30.0% | |
| GCTAATCAGAAAAAAATCTC+AGG | + | chr1.1:35168450-35168469 | MS.gene073645:CDS | 30.0% | |
| GTTACTAATATTAATGGTGC+TGG | + | chr1.1:35168371-35168390 | MS.gene073645:intron | 30.0% | |
| TCTAAGAAGTTGAAAATGCA+AGG | + | chr1.1:35169378-35169397 | MS.gene073645:CDS | 30.0% | |
| TGACATTTCAACTTCAAAAC+TGG | - | chr1.1:35168682-35168701 | None:intergenic | 30.0% | |
| TGGCAAGAGAAAAATGAATA+TGG | + | chr1.1:35168419-35168438 | MS.gene073645:CDS | 30.0% | |
| TGTAGCTCCACAAAATATTT+GGG | - | chr1.1:35168083-35168102 | None:intergenic | 30.0% | |
| TTGAGAAATTCAAGTACCAA+AGG | - | chr1.1:35169671-35169690 | None:intergenic | 30.0% | |
| ! | AATATTTTGTGGAGCTACAT+TGG | + | chr1.1:35168084-35168103 | MS.gene073645:intron | 30.0% |
| ! | CCTCTTTTCATAAATTCCAT+TGG | - | chr1.1:35169762-35169781 | None:intergenic | 30.0% |
| ! | GTGCTAAACAAGATGTATTT+TGG | + | chr1.1:35168147-35168166 | MS.gene073645:intron | 30.0% |
| !! | TAAACAAGATGTATTTTGGG+TGG | + | chr1.1:35168151-35168170 | MS.gene073645:intron | 30.0% |
| ACATCTCAAAGGAAAAAAGG+TGG | + | chr1.1:35168399-35168418 | MS.gene073645:intron | 35.0% | |
| ATTGAAACAGAGGATACCAA+GGG | + | chr1.1:35169110-35169129 | MS.gene073645:intron | 35.0% | |
| ATTGCGAAATCTCTTATACG+TGG | + | chr1.1:35168215-35168234 | MS.gene073645:intron | 35.0% | |
| CCAAAAATACATCTACTCAG+GGG | + | chr1.1:35169576-35169595 | MS.gene073645:CDS | 35.0% | |
| CCCAAAAATACATCTACTCA+GGG | + | chr1.1:35169575-35169594 | MS.gene073645:CDS | 35.0% | |
| CCTTCTTTATAACCTAGTCA+AGG | - | chr1.1:35169489-35169508 | None:intergenic | 35.0% | |
| CCTTGACTAGGTTATAAAGA+AGG | + | chr1.1:35169486-35169505 | MS.gene073645:intron | 35.0% | |
| GAGACAGCAAGATTTATAGT+TGG | + | chr1.1:35169165-35169184 | MS.gene073645:CDS | 35.0% | |
| GCTAAGAGCAAAGACATTAA+AGG | + | chr1.1:35169531-35169550 | MS.gene073645:CDS | 35.0% | |
| TATTCTCTCGAAAAATCCCT+TGG | - | chr1.1:35169129-35169148 | None:intergenic | 35.0% | |
| TATTGAAACAGAGGATACCA+AGG | + | chr1.1:35169109-35169128 | MS.gene073645:intron | 35.0% | |
| TGTAACTTTGCAGGTAGTAA+TGG | + | chr1.1:35168570-35168589 | MS.gene073645:intron | 35.0% | |
| TTAAAGGAACTTCCAGTTCT+AGG | + | chr1.1:35169547-35169566 | MS.gene073645:CDS | 35.0% | |
| TTACGCCAAAGGTCTTTATT+TGG | + | chr1.1:35168707-35168726 | MS.gene073645:CDS | 35.0% | |
| ! | ACATCTTGTTTAGCACCATT+TGG | - | chr1.1:35168143-35168162 | None:intergenic | 35.0% |
| ! | CATCTTGTTTAGCACCATTT+GGG | - | chr1.1:35168142-35168161 | None:intergenic | 35.0% |
| ! | CCCTGAGTAGATGTATTTTT+GGG | - | chr1.1:35169578-35169597 | None:intergenic | 35.0% |
| ! | TCAAAACTGGGATTTTCCAA+AGG | - | chr1.1:35168669-35168688 | None:intergenic | 35.0% |
| AAATCATCTACTGTGACCAC+TGG | + | chr1.1:35169234-35169253 | MS.gene073645:CDS | 40.0% | |
| AACTTTGCAGGTAGTAATGG+AGG | + | chr1.1:35168573-35168592 | MS.gene073645:intron | 40.0% | |
| ATGTCACAATCTTACGCCAA+AGG | + | chr1.1:35168696-35168715 | MS.gene073645:CDS | 40.0% | |
| GCCCAAAAATACATCTACTC+AGG | + | chr1.1:35169574-35169593 | MS.gene073645:CDS | 40.0% | |
| GGTCACAGTAGATGATTTAG+TGG | - | chr1.1:35169232-35169251 | None:intergenic | 40.0% | |
| GTAGTCTTTACTCTCCCAAA+TGG | + | chr1.1:35168125-35168144 | MS.gene073645:intron | 40.0% | |
| TATCATTACTCGAGCAAGAG+AGG | + | chr1.1:35169347-35169366 | MS.gene073645:CDS | 40.0% | |
| TGCTGGTACATACATCTCAA+AGG | + | chr1.1:35168388-35168407 | MS.gene073645:intron | 40.0% | |
| TTTGGGAGAGTAAAGACTAC+TGG | - | chr1.1:35168125-35168144 | None:intergenic | 40.0% | |
| ! | CCCCTGAGTAGATGTATTTT+TGG | - | chr1.1:35169579-35169598 | None:intergenic | 40.0% |
| ! | GAGGGTTCCCAAATATTTTG+TGG | + | chr1.1:35168073-35168092 | MS.gene073645:intron | 40.0% |
| ! | TGGAGGAGGTATGATTAAGA+AGG | + | chr1.1:35168590-35168609 | MS.gene073645:CDS | 40.0% |
| GCTAAATGTCTTCCAACCAG+TGG | - | chr1.1:35169253-35169272 | None:intergenic | 45.0% | |
| ! | TTTGCAGGTAGTAATGGAGG+AGG | + | chr1.1:35168576-35168595 | MS.gene073645:intron | 45.0% |
| !!! | AATTTTTTAACATTTTTAAA+AGG | - | chr1.1:35168009-35168028 | None:intergenic | 5.0% |
| !!! | TAATAATTTCATTTTTTTTA+AGG | + | chr1.1:35168767-35168786 | MS.gene073645:intron | 5.0% |
| CATCTACTGTGACCACTGGT+TGG | + | chr1.1:35169238-35169257 | MS.gene073645:CDS | 50.0% | |
| TGGAGCGTGACGGTGATATT+TGG | + | chr1.1:35168171-35168190 | MS.gene073645:intron | 50.0% | |
| ! | TTTTGGGCAGCTCCTAGAAC+TGG | - | chr1.1:35169562-35169581 | None:intergenic | 50.0% |
| !! | GTATTTTGGGTGGAGCGTGA+CGG | + | chr1.1:35168161-35168180 | MS.gene073645:intron | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 35167955 | 35169790 | 35167955 | ID=MS.gene073645 |
| chr1.1 | mRNA | 35167955 | 35169790 | 35167955 | ID=MS.gene073645.t1;Parent=MS.gene073645 |
| chr1.1 | exon | 35167955 | 35167965 | 35167955 | ID=MS.gene073645.t1.exon1;Parent=MS.gene073645.t1 |
| chr1.1 | CDS | 35167955 | 35167965 | 35167955 | ID=cds.MS.gene073645.t1;Parent=MS.gene073645.t1 |
| chr1.1 | exon | 35168410 | 35168471 | 35168410 | ID=MS.gene073645.t1.exon2;Parent=MS.gene073645.t1 |
| chr1.1 | CDS | 35168410 | 35168471 | 35168410 | ID=cds.MS.gene073645.t1;Parent=MS.gene073645.t1 |
| chr1.1 | exon | 35168583 | 35168728 | 35168583 | ID=MS.gene073645.t1.exon3;Parent=MS.gene073645.t1 |
| chr1.1 | CDS | 35168583 | 35168728 | 35168583 | ID=cds.MS.gene073645.t1;Parent=MS.gene073645.t1 |
| chr1.1 | exon | 35169120 | 35169399 | 35169120 | ID=MS.gene073645.t1.exon4;Parent=MS.gene073645.t1 |
| chr1.1 | CDS | 35169120 | 35169399 | 35169120 | ID=cds.MS.gene073645.t1;Parent=MS.gene073645.t1 |
| chr1.1 | exon | 35169496 | 35169597 | 35169496 | ID=MS.gene073645.t1.exon5;Parent=MS.gene073645.t1 |
| chr1.1 | CDS | 35169496 | 35169597 | 35169496 | ID=cds.MS.gene073645.t1;Parent=MS.gene073645.t1 |
| chr1.1 | exon | 35169753 | 35169790 | 35169753 | ID=MS.gene073645.t1.exon6;Parent=MS.gene073645.t1 |
| chr1.1 | CDS | 35169753 | 35169790 | 35169753 | ID=cds.MS.gene073645.t1;Parent=MS.gene073645.t1 |
| Gene Sequence |
| Protein sequence |
