AlfalfaGEDB | MS.gene073645

M. sativa cultivar XinJiangDaYe / MS.gene073645

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF02362.24

Nr:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073645.t1	RHN78231.1	89	154	17	0	14	167	144	297	4.20E-69	271.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073645.t1	A0A396JNG9	89.0	154	17	0	14	167	144	297	3.0e-69	271.2

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TFs/TRs:

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Gene ID	Type	Classification
MS.gene073645.t1	TF	B3

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network:

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Gene1	Gene2	correlation coefficient	p_value	FDR
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PPI:

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene073645.t1	MTR_1g035640	52.000	200	87	4	8	206	139	330	3.61e-55	182
MS.gene073645.t1	MTR_1g035620	50.746	201	61	10	5	199	141	309	4.88e-45	154
MS.gene073645.t1	MTR_1g035490	44.388	196	82	4	6	199	61	231	6.04e-44	150
MS.gene073645.t1	MTR_1g035570	45.361	194	93	4	20	211	156	338	1.50e-41	145
MS.gene073645.t1	MTR_1g050845	51.724	203	64	8	5	201	128	302	2.80e-41	149
MS.gene073645.t1	MTR_1g035650	89.855	69	7	0	5	73	135	203	2.44e-37	129
MS.gene073645.t1	MTR_6g061070	40.777	206	103	6	9	200	171	371	7.35e-36	131
MS.gene073645.t1	MTR_5g044670	31.875	160	94	5	20	167	161	317	7.62e-18	80.5
MS.gene073645.t1	MTR_1g035540	57.447	47	20	0	118	164	31	77	2.02e-12	62.4
MS.gene073645.t1	MTR_8g103940	35.433	127	72	5	47	173	232	348	1.30e-11	63.5

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Find 28 sgRNAs with CRISPR-Local

Find 86 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAATCATCTACTGTGACCAC+TGG	0.561649	1.1:+35169234	MS.gene073645:CDS
AACTTTGCAGGTAGTAATGG+AGG	0.609546	1.1:+35168573	MS.gene073645:intron
ACATACCAAATAAAGACCTT+TGG	0.466172	1.1:-35168712	None:intergenic
AGCAAGAAATTATCATCCTT+TGG	0.369605	1.1:+35168650	MS.gene073645:CDS
ATATATACAGGATGTACCAA+TGG	0.596542	1.1:+35169743	MS.gene073645:intron
ATGATAATAGTGAATAAAAG+TGG	0.508803	1.1:-35169330	None:intergenic
ATGTCACAATCTTACGCCAA+AGG	0.623356	1.1:+35168696	MS.gene073645:CDS
ATTGAAACAGAGGATACCAA+GGG	0.683744	1.1:+35169110	MS.gene073645:intron
ATTTATAGTTGGTGAAGATA+TGG	0.400385	1.1:+35169176	MS.gene073645:CDS
CATCTACTGTGACCACTGGT+TGG	0.552617	1.1:+35169238	MS.gene073645:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AATTTATTTGACCCTTGACT+AGG	+	chr1.1:35169474-35169493	MS.gene073645:intron	30.0%
ACAAACTGATATTGAAACAG+AGG	+	chr1.1:35169100-35169119	MS.gene073645:intron	30.0%
ACATACCAAATAAAGACCTT+TGG	-	chr1.1:35168715-35168734	None:intergenic	30.0%
AGCAAGAAATTATCATCCTT+TGG	+	chr1.1:35168650-35168669	MS.gene073645:CDS	30.0%
ATATATACAGGATGTACCAA+TGG	+	chr1.1:35169743-35169762	MS.gene073645:intron	30.0%
ATGTAGCTCCACAAAATATT+TGG	-	chr1.1:35168084-35168103	None:intergenic	30.0%
CAAGGGTCAAATAAATTAAG+TGG	-	chr1.1:35169471-35169490	None:intergenic	30.0%
CATACATCTCAAAGGAAAAA+AGG	+	chr1.1:35168396-35168415	MS.gene073645:intron	30.0%
CCAATGGAATTTATGAAAAG+AGG	+	chr1.1:35169759-35169778	MS.gene073645:CDS	30.0%
CTTCTTTATAACCTAGTCAA+GGG	-	chr1.1:35169488-35169507	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
chr1.1	gene	35167955	35169790	35167955	ID=MS.gene073645
chr1.1	mRNA	35167955	35169790	35167955	ID=MS.gene073645.t1;Parent=MS.gene073645
chr1.1	exon	35167955	35167965	35167955	ID=MS.gene073645.t1.exon1;Parent=MS.gene073645.t1
chr1.1	CDS	35167955	35167965	35167955	ID=cds.MS.gene073645.t1;Parent=MS.gene073645.t1
chr1.1	exon	35168410	35168471	35168410	ID=MS.gene073645.t1.exon2;Parent=MS.gene073645.t1
chr1.1	CDS	35168410	35168471	35168410	ID=cds.MS.gene073645.t1;Parent=MS.gene073645.t1
chr1.1	exon	35168583	35168728	35168583	ID=MS.gene073645.t1.exon3;Parent=MS.gene073645.t1
chr1.1	CDS	35168583	35168728	35168583	ID=cds.MS.gene073645.t1;Parent=MS.gene073645.t1
chr1.1	exon	35169120	35169399	35169120	ID=MS.gene073645.t1.exon4;Parent=MS.gene073645.t1
chr1.1	CDS	35169120	35169399	35169120	ID=cds.MS.gene073645.t1;Parent=MS.gene073645.t1
chr1.1	exon	35169496	35169597	35169496	ID=MS.gene073645.t1.exon5;Parent=MS.gene073645.t1
chr1.1	CDS	35169496	35169597	35169496	ID=cds.MS.gene073645.t1;Parent=MS.gene073645.t1
chr1.1	exon	35169753	35169790	35169753	ID=MS.gene073645.t1.exon6;Parent=MS.gene073645.t1
chr1.1	CDS	35169753	35169790	35169753	ID=cds.MS.gene073645.t1;Parent=MS.gene073645.t1

Gene Sequence

>MS.gene073645

ATGGAGAGCTTGTAAGCATTTTATATTAACTTCATGTTTATTTCTTTCATTCCTTTTAAAAATGTTAAAAAATTACACAATCCAACAACTATACTTATTCAAAAATATTTATCTTGCAGAGGGTTCCCAAATATTTTGTGGAGCTACATTGGAAAAATGTATCAAATCCAGTAGTCTTTACTCTCCCAAATGGTGCTAAACAAGATGTATTTTGGGTGGAGCGTGACGGTGATATTTGGTTTCATAAAAATTGGGAAAAAATTGCGAAATCTCTTATACGTGGACATGTTCTAGTTTTTAAATATAAAGGAGGATCATGTTTCAAAGTTGAAATATTTGGTTGCAATACATTGGAAATAGACTATTCCAATATAAAAGTTGAAGCTGAAGAAGTTGTTACAGTGAGTGATGACAATGTTACTAATATTAATGGTGCTGGTACATACATCTCAAAGGAAAAAAGGTGGCAAGAGAAAAATGAATATGGATCTTGATGCTAATCAGAAAAAAATCTCAGGTAATTACATCTAATAACTTTTTTTTAAGATTTTTATTTCATATTTTTACTTGTAAATGTTTAAATTAGTTTTTTTTATTTTTTTTATTTTTTATATATGTAACTTTGCAGGTAGTAATGGAGGAGGTATGATTAAGAAGGCTAAAAAATGCTTAAGAAATGAAACTGTTAAAGAGAGAGCAAGAAATTATCATCCTTTGGAAAATCCCAGTTTTGAAGTTGAAATGTCACAATCTTACGCCAAAGGTCTTTATTTGGTATGTTTTCTATCAAAATTCTCTATTTTGGCTTTTATTAATAATTTCATTTTTTTTAAGGAATATTTCTAATAAATTTTATTTTGAACTGTGAATTGATGATCTTCACTTTTTTTTTTGCTAAAAATAACTGACTTTAATATTAATAATTTATATTAGTTTATCATTTAAAAAAATAAAAAAAATTATATTTGTTTATCATAATGAAAATAATTTTCTAATAAATAAAATATCTATCAATTATTTTTAAGAGAAAATTGTCAAAATAGGATTCATATTTTAAGCCTTCATTAGATTTTGTTTTATAAAAGTGTAACAAATGGATAAAAATATATAAAAGTAATAGTAGCAAGTATTTATTTTTTTTTTTAACAAACTGATATTGAAACAGAGGATACCAAGGGATTTTTCGAGAGAATACTTGAAAAAGTTGGAAGAGACAGCAAGATTTATAGTTGGTGAAGATATGGCTATAGAAGTGAAGTTGAATTTTGATGATACCACTAAATCATCTACTGTGACCACTGGTTGGAAGACATTTAGCAAAAAATATAATTTGCAAGTTGGTGATGTATGTAAATTTGTGATGACTCAATGCCAACCACTTTTATTCACTATTATCATTACTCGAGCAAGAGAGGAACCTAACTCTAAGAAGTTGAAAATGCAAGGTCTTTTATTTTTTTTCTTATTAAAAATGCTTTTAATTGATATTTGAATAACATCTATAGCTTTGTCTCCACTTAATTTATTTGACCCTTGACTAGGTTATAAAGAAGGGATCTCTTGTAGTGATGACAATGCTAAGAGCAAAGACATTAAAGGAACTTCCAGTTCTAGGAGCTGCCCAAAAATACATCTACTCAGGGGTATTTTTCTAACTTTAAAGTTATCAATTTTAATAAATTATTTTCAATTATATGTTTGAACATTGTTTTTCCTTTGGTACTTGAATTTCTCAATAACTTACAAGTCTTTGTACTATGATGTTTTTAGTTGTATATTTAAACTTTTATATATACAGGATGTACCAATGGAATTTATGAAAAGAGGATGTCATGA

Protein sequence

>MS.gene073645.t1

MESLKKGGKRKMNMDLDANQKKISGSNGGGMIKKAKKCLRNETVKERARNYHPLENPSFEVEMSQSYAKGLYLRIPRDFSREYLKKLEETARFIVGEDMAIEVKLNFDDTTKSSTVTTGWKTFSKKYNLQVGDVCKFVMTQCQPLLFTIIITRAREEPNSKKLKMQGYKEGISCSDDNAKSKDIKGTSSSRSCPKIHLLRGCTNGIYEKRMS