Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074199.t1 | RHN50483.1 | 93.9 | 346 | 21 | 0 | 1 | 346 | 11 | 356 | 7.30E-188 | 666.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074199.t1 | Q9FIM0 | 27.6 | 275 | 176 | 8 | 6 | 261 | 6 | 276 | 2.6e-16 | 87.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074199.t1 | A0A072U6V5 | 93.9 | 346 | 21 | 0 | 1 | 346 | 1 | 346 | 5.2e-188 | 666.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene074199.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074199.t1 | MTR_6g018920 | 93.931 | 346 | 21 | 0 | 1 | 346 | 1 | 346 | 0.0 | 688 |
| MS.gene074199.t1 | MTR_1g114730 | 74.157 | 356 | 73 | 3 | 1 | 346 | 1 | 347 | 0.0 | 521 |
| MS.gene074199.t1 | MTR_6g005450 | 66.097 | 351 | 110 | 4 | 1 | 346 | 1 | 347 | 3.14e-160 | 452 |
| MS.gene074199.t1 | MTR_6g005440 | 61.850 | 346 | 101 | 3 | 1 | 346 | 1 | 315 | 1.70e-149 | 424 |
| MS.gene074199.t1 | MTR_4g107170 | 41.935 | 155 | 87 | 1 | 1 | 155 | 1 | 152 | 1.92e-37 | 135 |
| MS.gene074199.t1 | MTR_2g049610 | 43.226 | 155 | 82 | 1 | 1 | 155 | 1 | 149 | 3.36e-37 | 134 |
| MS.gene074199.t1 | MTR_4g045977 | 37.725 | 167 | 101 | 1 | 1 | 167 | 1 | 164 | 9.08e-34 | 129 |
| MS.gene074199.t1 | MTR_2g049530 | 37.662 | 154 | 91 | 2 | 3 | 155 | 5 | 154 | 1.22e-30 | 115 |
| MS.gene074199.t1 | MTR_2g049630 | 38.065 | 155 | 91 | 2 | 2 | 155 | 4 | 154 | 1.50e-28 | 114 |
| MS.gene074199.t1 | MTR_1g115290 | 32.231 | 121 | 81 | 1 | 44 | 164 | 8 | 127 | 5.67e-21 | 88.6 |
| MS.gene074199.t1 | MTR_0061s0010 | 76.744 | 43 | 10 | 0 | 29 | 71 | 1 | 43 | 7.39e-18 | 79.3 |
| MS.gene074199.t1 | MTR_0061s0010 | 71.795 | 39 | 11 | 0 | 131 | 169 | 42 | 80 | 8.15e-11 | 59.7 |
| MS.gene074199.t1 | MTR_3g466980 | 27.044 | 159 | 102 | 5 | 1 | 151 | 1 | 153 | 9.07e-16 | 75.9 |
| MS.gene074199.t1 | MTR_3g102570 | 29.936 | 157 | 94 | 4 | 1 | 157 | 1 | 141 | 1.57e-15 | 76.6 |
| MS.gene074199.t1 | MTR_3g031100 | 25.121 | 207 | 138 | 6 | 1 | 197 | 1 | 200 | 1.87e-15 | 75.5 |
| MS.gene074199.t1 | MTR_3g031240 | 31.746 | 126 | 79 | 3 | 1 | 125 | 1 | 120 | 3.58e-15 | 73.2 |
| MS.gene074199.t1 | MTR_5g075380 | 29.518 | 166 | 101 | 6 | 1 | 157 | 1 | 159 | 2.35e-14 | 70.5 |
| MS.gene074199.t1 | MTR_3g466890 | 32.099 | 162 | 92 | 7 | 3 | 154 | 2 | 155 | 3.25e-14 | 70.1 |
| MS.gene074199.t1 | MTR_3g065100 | 27.309 | 249 | 134 | 9 | 1 | 215 | 1 | 236 | 3.96e-14 | 71.6 |
| MS.gene074199.t1 | MTR_4g032620 | 37.607 | 117 | 58 | 5 | 1 | 116 | 1 | 103 | 4.59e-14 | 71.2 |
| MS.gene074199.t1 | MTR_4g063790 | 28.750 | 160 | 99 | 5 | 1 | 151 | 1 | 154 | 8.79e-14 | 68.9 |
| MS.gene074199.t1 | MTR_8g086290 | 31.293 | 147 | 81 | 4 | 2 | 136 | 12 | 150 | 4.31e-13 | 67.4 |
| MS.gene074199.t1 | MTR_3g466830 | 33.636 | 110 | 66 | 3 | 3 | 111 | 2 | 105 | 1.32e-12 | 65.5 |
| MS.gene074199.t1 | MTR_5g041650 | 29.907 | 107 | 68 | 2 | 1 | 106 | 38 | 138 | 1.87e-12 | 68.2 |
| MS.gene074199.t1 | MTR_5g021270 | 29.375 | 160 | 96 | 5 | 1 | 155 | 1 | 148 | 2.15e-12 | 66.2 |
| MS.gene074199.t1 | MTR_5g021270 | 29.375 | 160 | 96 | 5 | 1 | 155 | 1 | 148 | 2.22e-12 | 66.2 |
| MS.gene074199.t1 | MTR_7g075850 | 26.144 | 153 | 99 | 4 | 1 | 150 | 1 | 142 | 2.75e-12 | 66.2 |
| MS.gene074199.t1 | MTR_4g084740 | 26.415 | 159 | 106 | 2 | 1 | 159 | 1 | 148 | 5.01e-12 | 66.6 |
| MS.gene074199.t1 | MTR_8g079502 | 38.272 | 81 | 38 | 2 | 1 | 69 | 1 | 81 | 5.77e-12 | 63.9 |
| MS.gene074199.t1 | MTR_5g066960 | 46.552 | 58 | 31 | 0 | 1 | 58 | 1 | 58 | 5.81e-12 | 60.8 |
| MS.gene074199.t1 | MTR_8g087860 | 28.369 | 141 | 92 | 3 | 1 | 141 | 17 | 148 | 7.55e-12 | 65.1 |
| MS.gene074199.t1 | MTR_8g033270 | 26.667 | 150 | 101 | 3 | 1 | 150 | 1 | 141 | 1.09e-11 | 64.7 |
| MS.gene074199.t1 | MTR_2g016210 | 32.407 | 108 | 65 | 4 | 2 | 108 | 3 | 103 | 1.18e-11 | 64.3 |
| MS.gene074199.t1 | MTR_3g467080 | 26.220 | 164 | 106 | 7 | 1 | 155 | 1 | 158 | 1.25e-11 | 62.8 |
| MS.gene074199.t1 | MTR_2g017865 | 27.083 | 144 | 90 | 3 | 1 | 141 | 16 | 147 | 1.82e-11 | 63.9 |
| MS.gene074199.t1 | MTR_1g090710 | 25.455 | 165 | 103 | 5 | 1 | 154 | 1 | 156 | 2.25e-11 | 62.0 |
| MS.gene074199.t1 | MTR_4g131030 | 41.667 | 60 | 35 | 0 | 1 | 60 | 1 | 60 | 2.50e-11 | 62.8 |
| MS.gene074199.t1 | MTR_1g047550 | 41.667 | 60 | 35 | 0 | 1 | 60 | 1 | 60 | 2.59e-11 | 62.0 |
| MS.gene074199.t1 | MTR_1g053070 | 53.061 | 49 | 23 | 0 | 1 | 49 | 1 | 49 | 3.04e-11 | 63.2 |
| MS.gene074199.t1 | MTR_1g054265 | 43.333 | 60 | 34 | 0 | 1 | 60 | 1 | 60 | 3.61e-11 | 61.6 |
| MS.gene074199.t1 | MTR_1g029670 | 27.027 | 148 | 88 | 5 | 1 | 144 | 1 | 132 | 4.00e-11 | 61.6 |
| MS.gene074199.t1 | MTR_2g105290 | 26.429 | 140 | 99 | 1 | 1 | 136 | 11 | 150 | 4.02e-11 | 61.6 |
| MS.gene074199.t1 | MTR_5g047580 | 28.125 | 128 | 79 | 4 | 1 | 128 | 1 | 115 | 4.30e-11 | 60.8 |
| MS.gene074199.t1 | MTR_1g090697 | 26.087 | 161 | 106 | 5 | 1 | 154 | 1 | 155 | 4.43e-11 | 60.8 |
| MS.gene074199.t1 | MTR_1g090783 | 26.316 | 152 | 93 | 6 | 3 | 144 | 2 | 144 | 5.23e-11 | 60.8 |
| MS.gene074199.t1 | MTR_2g461710 | 46.939 | 49 | 26 | 0 | 1 | 49 | 1 | 49 | 6.12e-11 | 57.8 |
| MS.gene074199.t1 | MTR_3g088615 | 37.662 | 77 | 42 | 1 | 1 | 77 | 1 | 71 | 6.34e-11 | 61.2 |
| MS.gene074199.t1 | MTR_4g109830 | 28.022 | 182 | 99 | 6 | 1 | 168 | 1 | 164 | 6.65e-11 | 62.0 |
| MS.gene074199.t1 | MTR_2g009890 | 53.061 | 49 | 23 | 0 | 1 | 49 | 1 | 49 | 7.79e-11 | 62.4 |
| MS.gene074199.t1 | MTR_7g075870 | 27.273 | 154 | 96 | 4 | 1 | 150 | 1 | 142 | 8.56e-11 | 61.2 |
| MS.gene074199.t1 | MTR_6g015975 | 28.966 | 145 | 92 | 4 | 1 | 144 | 1 | 135 | 9.17e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074199.t1 | AT5G55690 | 39.073 | 151 | 84 | 2 | 1 | 150 | 1 | 144 | 2.45e-25 | 103 |
| MS.gene074199.t1 | AT5G55690 | 39.073 | 151 | 84 | 2 | 1 | 150 | 1 | 144 | 2.45e-25 | 103 |
| MS.gene074199.t1 | AT5G58890 | 36.301 | 146 | 90 | 2 | 6 | 150 | 6 | 149 | 2.32e-20 | 90.1 |
| MS.gene074199.t1 | AT3G18650 | 26.549 | 339 | 200 | 11 | 16 | 333 | 31 | 341 | 4.41e-18 | 84.7 |
| MS.gene074199.t1 | AT1G22590 | 31.056 | 161 | 100 | 3 | 1 | 156 | 1 | 155 | 2.25e-16 | 76.3 |
| MS.gene074199.t1 | AT1G65300 | 29.747 | 158 | 97 | 7 | 5 | 154 | 4 | 155 | 1.69e-14 | 73.2 |
| MS.gene074199.t1 | AT1G65330 | 34.234 | 111 | 66 | 4 | 3 | 112 | 2 | 106 | 9.14e-14 | 71.2 |
| MS.gene074199.t1 | AT5G27960 | 26.180 | 233 | 131 | 9 | 5 | 202 | 3 | 229 | 1.34e-13 | 71.2 |
| MS.gene074199.t1 | AT5G26650 | 32.110 | 109 | 67 | 3 | 5 | 112 | 3 | 105 | 3.25e-13 | 70.5 |
| MS.gene074199.t1 | AT5G23260 | 25.263 | 190 | 125 | 4 | 1 | 186 | 1 | 177 | 5.01e-13 | 68.2 |
| MS.gene074199.t1 | AT3G05860 | 38.961 | 77 | 41 | 3 | 1 | 77 | 1 | 71 | 9.62e-13 | 67.0 |
| MS.gene074199.t1 | AT3G05860 | 38.961 | 77 | 41 | 3 | 1 | 77 | 1 | 71 | 1.21e-12 | 67.4 |
| MS.gene074199.t1 | AT3G05860 | 38.961 | 77 | 41 | 3 | 1 | 77 | 1 | 71 | 1.37e-12 | 67.4 |
| MS.gene074199.t1 | AT5G23260 | 25.949 | 158 | 101 | 3 | 1 | 155 | 17 | 161 | 1.41e-12 | 67.4 |
| MS.gene074199.t1 | AT5G23260 | 26.144 | 153 | 97 | 3 | 1 | 150 | 1 | 140 | 1.50e-12 | 67.0 |
| MS.gene074199.t1 | AT4G11880 | 40.000 | 75 | 45 | 0 | 1 | 75 | 1 | 75 | 2.02e-12 | 65.5 |
| MS.gene074199.t1 | AT4G11880 | 40.000 | 75 | 45 | 0 | 1 | 75 | 1 | 75 | 2.02e-12 | 65.5 |
| MS.gene074199.t1 | AT5G23260 | 26.144 | 153 | 97 | 3 | 1 | 150 | 17 | 156 | 2.31e-12 | 67.0 |
| MS.gene074199.t1 | AT5G26580 | 49.153 | 59 | 30 | 0 | 1 | 59 | 1 | 59 | 4.27e-12 | 67.0 |
| MS.gene074199.t1 | AT3G57230 | 52.000 | 50 | 24 | 0 | 1 | 50 | 1 | 50 | 5.10e-12 | 63.5 |
| MS.gene074199.t1 | AT4G11880 | 40.000 | 75 | 45 | 0 | 1 | 75 | 1 | 75 | 5.67e-12 | 65.1 |
| MS.gene074199.t1 | AT4G11880 | 40.000 | 75 | 45 | 0 | 1 | 75 | 1 | 75 | 5.67e-12 | 65.1 |
| MS.gene074199.t1 | AT2G45650 | 30.464 | 151 | 93 | 5 | 1 | 150 | 1 | 140 | 7.91e-12 | 65.1 |
| MS.gene074199.t1 | AT5G62165 | 32.990 | 97 | 57 | 1 | 1 | 97 | 1 | 89 | 9.16e-12 | 61.6 |
| MS.gene074199.t1 | AT2G03060 | 28.972 | 107 | 69 | 2 | 1 | 106 | 1 | 101 | 9.52e-12 | 65.9 |
| MS.gene074199.t1 | AT2G03060 | 28.972 | 107 | 69 | 2 | 1 | 106 | 1 | 101 | 9.52e-12 | 65.9 |
| MS.gene074199.t1 | AT2G03060 | 28.972 | 107 | 69 | 2 | 1 | 106 | 1 | 101 | 9.52e-12 | 65.9 |
| MS.gene074199.t1 | AT5G62165 | 27.500 | 160 | 96 | 4 | 1 | 154 | 1 | 146 | 1.13e-11 | 62.8 |
| MS.gene074199.t1 | AT5G62165 | 27.500 | 160 | 96 | 4 | 1 | 154 | 1 | 146 | 1.13e-11 | 62.8 |
| MS.gene074199.t1 | AT5G62165 | 27.500 | 160 | 96 | 4 | 1 | 154 | 1 | 146 | 1.13e-11 | 62.8 |
| MS.gene074199.t1 | AT5G62165 | 27.500 | 160 | 96 | 4 | 1 | 154 | 1 | 146 | 1.13e-11 | 62.8 |
| MS.gene074199.t1 | AT5G62165 | 27.500 | 160 | 96 | 4 | 1 | 154 | 1 | 146 | 1.13e-11 | 62.8 |
| MS.gene074199.t1 | AT2G03060 | 28.972 | 107 | 69 | 2 | 1 | 106 | 1 | 101 | 1.13e-11 | 65.9 |
| MS.gene074199.t1 | AT3G57230 | 52.000 | 50 | 24 | 0 | 1 | 50 | 1 | 50 | 1.32e-11 | 64.3 |
| MS.gene074199.t1 | AT3G57230 | 52.000 | 50 | 24 | 0 | 1 | 50 | 1 | 50 | 1.32e-11 | 64.3 |
| MS.gene074199.t1 | AT2G34440 | 26.471 | 170 | 104 | 5 | 1 | 162 | 1 | 157 | 1.43e-11 | 62.8 |
| MS.gene074199.t1 | AT3G57390 | 40.244 | 82 | 41 | 1 | 1 | 82 | 1 | 74 | 1.48e-11 | 63.9 |
| MS.gene074199.t1 | AT1G18750 | 28.972 | 107 | 69 | 2 | 1 | 106 | 1 | 101 | 1.54e-11 | 65.5 |
| MS.gene074199.t1 | AT2G22630 | 46.552 | 58 | 31 | 0 | 1 | 58 | 1 | 58 | 1.59e-11 | 63.9 |
| MS.gene074199.t1 | AT2G22630 | 46.552 | 58 | 31 | 0 | 1 | 58 | 1 | 58 | 1.59e-11 | 63.9 |
| MS.gene074199.t1 | AT2G45660 | 27.957 | 186 | 111 | 6 | 1 | 176 | 1 | 173 | 1.74e-11 | 63.5 |
| MS.gene074199.t1 | AT1G18750 | 28.972 | 107 | 69 | 2 | 1 | 106 | 1 | 101 | 1.97e-11 | 65.1 |
| MS.gene074199.t1 | AT1G77980 | 22.642 | 159 | 112 | 2 | 1 | 159 | 1 | 148 | 2.05e-11 | 64.7 |
| MS.gene074199.t1 | AT1G18750 | 28.972 | 107 | 69 | 2 | 1 | 106 | 1 | 101 | 2.14e-11 | 65.1 |
| MS.gene074199.t1 | AT3G57390 | 40.244 | 82 | 41 | 1 | 1 | 82 | 1 | 74 | 2.52e-11 | 63.5 |
| MS.gene074199.t1 | AT3G54340 | 25.333 | 150 | 100 | 2 | 1 | 150 | 1 | 138 | 2.57e-11 | 63.2 |
| MS.gene074199.t1 | AT4G18960 | 25.342 | 146 | 94 | 4 | 2 | 144 | 18 | 151 | 2.60e-11 | 63.5 |
| MS.gene074199.t1 | AT5G62165 | 30.275 | 109 | 68 | 1 | 1 | 109 | 1 | 101 | 2.97e-11 | 61.6 |
| MS.gene074199.t1 | AT5G62165 | 30.275 | 109 | 68 | 1 | 1 | 109 | 1 | 101 | 2.97e-11 | 61.6 |
| MS.gene074199.t1 | AT5G62165 | 30.275 | 109 | 68 | 1 | 1 | 109 | 1 | 101 | 2.97e-11 | 61.6 |
| MS.gene074199.t1 | AT1G01530 | 24.153 | 236 | 151 | 7 | 1 | 218 | 6 | 231 | 3.00e-11 | 63.2 |
| MS.gene074199.t1 | AT2G28700 | 25.882 | 170 | 106 | 7 | 1 | 164 | 1 | 156 | 3.10e-11 | 64.3 |
| MS.gene074199.t1 | AT5G62165 | 30.275 | 109 | 68 | 1 | 1 | 109 | 1 | 101 | 3.14e-11 | 62.4 |
| MS.gene074199.t1 | AT5G62165 | 30.275 | 109 | 68 | 1 | 1 | 109 | 1 | 101 | 3.14e-11 | 62.4 |
| MS.gene074199.t1 | AT2G42830 | 26.380 | 163 | 104 | 5 | 1 | 159 | 16 | 166 | 3.35e-11 | 63.2 |
| MS.gene074199.t1 | AT2G45660 | 38.095 | 84 | 44 | 1 | 1 | 84 | 1 | 76 | 3.40e-11 | 61.2 |
| MS.gene074199.t1 | AT2G42830 | 26.380 | 163 | 104 | 5 | 1 | 159 | 16 | 166 | 4.02e-11 | 62.8 |
| MS.gene074199.t1 | AT1G22130 | 22.642 | 159 | 112 | 2 | 1 | 159 | 1 | 148 | 4.19e-11 | 63.9 |
| MS.gene074199.t1 | AT1G31640 | 28.829 | 111 | 71 | 3 | 3 | 112 | 2 | 105 | 4.46e-11 | 64.3 |
| MS.gene074199.t1 | AT5G62165 | 30.275 | 109 | 68 | 1 | 1 | 109 | 1 | 101 | 4.58e-11 | 62.0 |
| MS.gene074199.t1 | AT5G62165 | 30.275 | 109 | 68 | 1 | 1 | 109 | 1 | 101 | 4.58e-11 | 62.0 |
| MS.gene074199.t1 | AT5G62165 | 30.275 | 109 | 68 | 1 | 1 | 109 | 1 | 101 | 4.58e-11 | 62.0 |
| MS.gene074199.t1 | AT5G13790 | 51.020 | 49 | 24 | 0 | 1 | 49 | 1 | 49 | 4.81e-11 | 63.2 |
| MS.gene074199.t1 | AT4G09960 | 26.582 | 158 | 99 | 4 | 1 | 154 | 1 | 145 | 4.92e-11 | 62.4 |
| MS.gene074199.t1 | AT5G60910 | 28.758 | 153 | 95 | 4 | 1 | 150 | 1 | 142 | 4.94e-11 | 62.8 |
| MS.gene074199.t1 | AT4G09960 | 26.582 | 158 | 99 | 4 | 1 | 154 | 2 | 146 | 5.13e-11 | 62.4 |
| MS.gene074199.t1 | AT4G09960 | 26.582 | 158 | 99 | 4 | 1 | 154 | 11 | 155 | 5.26e-11 | 62.4 |
| MS.gene074199.t1 | AT4G11250 | 26.471 | 204 | 133 | 7 | 19 | 220 | 13 | 201 | 8.85e-11 | 62.8 |
Find 74 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACATGGATTTCAACTTAA+TGG | 0.109578 | 6.2:-14992825 | MS.gene074199:CDS |
| GCAAGCAGATTGATGTTAAT+TGG | 0.174718 | 6.2:-14993031 | MS.gene074199:CDS |
| GTGGTAGGTTTGAAAGATTC+TGG | 0.248071 | 6.2:+14992844 | None:intergenic |
| TTGTCTTTAGGCCATGTTTC+CGG | 0.275357 | 6.2:+14993605 | None:intergenic |
| ATTAGTGATAAAACCCCATT+AGG | 0.276525 | 6.2:-14993167 | MS.gene074199:CDS |
| GCATTTCCATTGTAATAGTA+TGG | 0.277693 | 6.2:+14992912 | None:intergenic |
| TATGCTAAAAGGAGATATTA+AGG | 0.280393 | 6.2:-14993327 | MS.gene074199:CDS |
| ATAAAACCCCATTAGGGTTT+TGG | 0.280522 | 6.2:-14993160 | MS.gene074199:CDS |
| CAAAGGATGCATATGCTAAA+AGG | 0.297288 | 6.2:-14993338 | MS.gene074199:CDS |
| CTTGGCTTTGAGGCCAAGTT+AGG | 0.322315 | 6.2:+14993254 | None:intergenic |
| GAAGTTGCCAAAACCCTAAT+GGG | 0.332063 | 6.2:+14993153 | None:intergenic |
| TCTTGAACATCATAGATCTT+AGG | 0.373714 | 6.2:+14993527 | None:intergenic |
| AATCCCGGGGCTTCAAAGTT+AGG | 0.381492 | 6.2:+14993635 | None:intergenic |
| CAAGCAGATTGATGTTAATT+GGG | 0.383614 | 6.2:-14993030 | MS.gene074199:CDS |
| TGAAGTTGCCAAAACCCTAA+TGG | 0.404084 | 6.2:+14993152 | None:intergenic |
| GATGAATCTTACAATTCTCT+TGG | 0.404689 | 6.2:-14993419 | MS.gene074199:CDS |
| TTGATGTTAATTGGGCCAAC+TGG | 0.408093 | 6.2:-14993022 | MS.gene074199:CDS |
| GGGCCGTGGAAGAATATCTA+TGG | 0.413157 | 6.2:-14993780 | MS.gene074199:CDS |
| AACATCAACATCACAAAGAA+TGG | 0.436469 | 6.2:+14993676 | None:intergenic |
| TTCTCCTAACTTTGAAGCCC+CGG | 0.437012 | 6.2:-14993639 | MS.gene074199:CDS |
| GGAAGAGAATGCATAGCAAC+AGG | 0.437355 | 6.2:+14992870 | None:intergenic |
| GCAAATGACCTCAATTGTTC+CGG | 0.442221 | 6.2:+14993395 | None:intergenic |
| CACAAGGTTGATAAATTTGT+AGG | 0.443122 | 6.2:-14993281 | MS.gene074199:CDS |
| ATCTGAAAATCGACATGAAA+AGG | 0.444904 | 6.2:-14992968 | MS.gene074199:CDS |
| AGCCCCGGGATTTGCTGAAC+CGG | 0.446619 | 6.2:-14993624 | MS.gene074199:CDS |
| ACATTTCAAAAGAGAAAGAA+TGG | 0.448242 | 6.2:-14993725 | MS.gene074199:CDS |
| GAAGTAGCTACTTGGCTTTG+AGG | 0.451246 | 6.2:+14993244 | None:intergenic |
| TTAGTGATAAAACCCCATTA+GGG | 0.454096 | 6.2:-14993166 | MS.gene074199:CDS |
| AAAGATGATGTATCCGTCTT+GGG | 0.455496 | 6.2:-14993441 | MS.gene074199:CDS |
| TATCTTCTCTTTGCGCACTT+TGG | 0.461986 | 6.2:+14993463 | None:intergenic |
| CTCTTGGACCGGAACAATTG+AGG | 0.477266 | 6.2:-14993403 | MS.gene074199:CDS |
| ATCTTACAATTCTCTTGGAC+CGG | 0.481367 | 6.2:-14993414 | MS.gene074199:CDS |
| TAAAGATGATGTATCCGTCT+TGG | 0.481458 | 6.2:-14993442 | MS.gene074199:CDS |
| TTTCCGGTTCAGCAAATCCC+GGG | 0.482987 | 6.2:+14993621 | None:intergenic |
| ATGAGATTGAAGTAGCTACT+TGG | 0.483532 | 6.2:+14993236 | None:intergenic |
| ATTTGTAGGTACTCCTAACT+TGG | 0.486423 | 6.2:-14993267 | MS.gene074199:CDS |
| TTATCACTAATATTCATCAA+TGG | 0.491553 | 6.2:+14993179 | None:intergenic |
| ATAGTATGAGCTTTGTGCAT+TGG | 0.491704 | 6.2:+14993094 | None:intergenic |
| TCTCCTAACTTTGAAGCCCC+GGG | 0.498039 | 6.2:-14993638 | MS.gene074199:CDS |
| ATGCTAAAAGGAGATATTAA+GGG | 0.498536 | 6.2:-14993326 | MS.gene074199:CDS |
| TTAACATCAATCTGCTTGCA+AGG | 0.499911 | 6.2:+14993035 | None:intergenic |
| GTTTCCGGTTCAGCAAATCC+CGG | 0.517428 | 6.2:+14993620 | None:intergenic |
| GCTTGCAAGGGTAAGATTGT+GGG | 0.518462 | 6.2:+14993048 | None:intergenic |
| TGCACAAAGCTCATACTATC+CGG | 0.522726 | 6.2:-14993090 | MS.gene074199:CDS |
| AGCTCATAAGTTCAACTACA+TGG | 0.526542 | 6.2:-14992778 | MS.gene074199:CDS |
| TGCTTGCAAGGGTAAGATTG+TGG | 0.530731 | 6.2:+14993047 | None:intergenic |
| ATCAACTCGGTTGGTCCAGT+TGG | 0.531175 | 6.2:+14993007 | None:intergenic |
| TTGTCGCCATACTATTACAA+TGG | 0.533945 | 6.2:-14992918 | MS.gene074199:CDS |
| CATAAGTTCAACTACATGGA+TGG | 0.541076 | 6.2:-14992774 | MS.gene074199:CDS |
| AACTTGATGCTTGTAATCAA+AGG | 0.542066 | 6.2:-14993355 | MS.gene074199:CDS |
| TAAGTTGAAATCCATGTGGT+AGG | 0.546009 | 6.2:+14992829 | None:intergenic |
| GCATTGGAAATCATAGAAGA+AGG | 0.559558 | 6.2:+14993110 | None:intergenic |
| GAGGTCATTTGCTAGCATGT+TGG | 0.565239 | 6.2:-14993384 | MS.gene074199:CDS |
| GATTTGCTGAACCGGAAACA+TGG | 0.565854 | 6.2:-14993616 | MS.gene074199:CDS |
| ACTATAACACAACAAGTGAC+AGG | 0.568787 | 6.2:-14993553 | MS.gene074199:CDS |
| GAAAATCGACATGAAAAGGA+AGG | 0.574406 | 6.2:-14992964 | MS.gene074199:CDS |
| TACCTACCTTCTTGAGACTC+CGG | 0.579791 | 6.2:+14993071 | None:intergenic |
| AGTTCAACTACATGGATGGA+AGG | 0.584745 | 6.2:-14992770 | MS.gene074199:CDS |
| TACTATCCGGAGTCTCAAGA+AGG | 0.586253 | 6.2:-14993077 | MS.gene074199:CDS |
| TCACTAATATTCATCAATGG+AGG | 0.597003 | 6.2:+14993182 | None:intergenic |
| TCCCCATAGAAAATGGGCCG+TGG | 0.598979 | 6.2:-14993794 | None:intergenic |
| TAACATCAATCTGCTTGCAA+GGG | 0.599195 | 6.2:+14993036 | None:intergenic |
| AACATTAGCATCAACTCGGT+TGG | 0.603209 | 6.2:+14992998 | None:intergenic |
| AGGTTTGAAAGATTCTGGAA+AGG | 0.625479 | 6.2:+14992849 | None:intergenic |
| AAGTTGCCAAAACCCTAATG+GGG | 0.636047 | 6.2:+14993154 | None:intergenic |
| AATCTTTCAAACCTACCACA+TGG | 0.651037 | 6.2:-14992840 | MS.gene074199:CDS |
| TTGTAAGATTCATCCCAAGA+CGG | 0.662155 | 6.2:+14993428 | None:intergenic |
| TTCCGGTTCAGCAAATCCCG+GGG | 0.665746 | 6.2:+14993622 | None:intergenic |
| ATCCGGAGTCTCAAGAAGGT+AGG | 0.678714 | 6.2:-14993073 | MS.gene074199:CDS |
| GCTCCATAGATATTCTTCCA+CGG | 0.693432 | 6.2:+14993777 | None:intergenic |
| ACGTAACATTAGCATCAACT+CGG | 0.701269 | 6.2:+14992994 | None:intergenic |
| AATAGCTCATGAATCACACA+AGG | 0.705760 | 6.2:-14993297 | MS.gene074199:CDS |
| ATGGCCTAAAGACAAAAGAG+AGG | 0.713382 | 6.2:-14993597 | MS.gene074199:CDS |
| CCATTAAGTTGAAATCCATG+TGG | 0.755933 | 6.2:+14992825 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATCACTAATATTCATCAA+TGG | + | chr6.2:14993370-14993389 | None:intergenic | 20.0% |
| ! | ACATTTCAAAAGAGAAAGAA+TGG | - | chr6.2:14992821-14992840 | MS.gene074199:CDS | 25.0% |
| ! | ATGCTAAAAGGAGATATTAA+GGG | - | chr6.2:14993220-14993239 | MS.gene074199:CDS | 25.0% |
| ! | TATGCTAAAAGGAGATATTA+AGG | - | chr6.2:14993219-14993238 | MS.gene074199:CDS | 25.0% |
| !! | TTGTGTTATAGTACTTTTGA+AGG | + | chr6.2:14992985-14993004 | None:intergenic | 25.0% |
| !!! | ATTCTTTCTCTTTTGAAATG+TGG | + | chr6.2:14992822-14992841 | None:intergenic | 25.0% |
| !!! | TGATGTTCAAGAGTATTTTA+AGG | - | chr6.2:14993030-14993049 | MS.gene074199:CDS | 25.0% |
| AACATCAACATCACAAAGAA+TGG | + | chr6.2:14992873-14992892 | None:intergenic | 30.0% | |
| AACTTGATGCTTGTAATCAA+AGG | - | chr6.2:14993191-14993210 | MS.gene074199:CDS | 30.0% | |
| ATCTGAAAATCGACATGAAA+AGG | - | chr6.2:14993578-14993597 | MS.gene074199:CDS | 30.0% | |
| ATTAGTGATAAAACCCCATT+AGG | - | chr6.2:14993379-14993398 | MS.gene074199:CDS | 30.0% | |
| CAAGCAGATTGATGTTAATT+GGG | - | chr6.2:14993516-14993535 | MS.gene074199:CDS | 30.0% | |
| GATGAATCTTACAATTCTCT+TGG | - | chr6.2:14993127-14993146 | MS.gene074199:CDS | 30.0% | |
| GCATTTCCATTGTAATAGTA+TGG | + | chr6.2:14993637-14993656 | None:intergenic | 30.0% | |
| TCACTAATATTCATCAATGG+AGG | + | chr6.2:14993367-14993386 | None:intergenic | 30.0% | |
| TCTTGAACATCATAGATCTT+AGG | + | chr6.2:14993022-14993041 | None:intergenic | 30.0% | |
| TTAGTGATAAAACCCCATTA+GGG | - | chr6.2:14993380-14993399 | MS.gene074199:CDS | 30.0% | |
| !! | CACAAGGTTGATAAATTTGT+AGG | - | chr6.2:14993265-14993284 | MS.gene074199:CDS | 30.0% |
| AAAGATGATGTATCCGTCTT+GGG | - | chr6.2:14993105-14993124 | MS.gene074199:CDS | 35.0% | |
| AATAGCTCATGAATCACACA+AGG | - | chr6.2:14993249-14993268 | MS.gene074199:CDS | 35.0% | |
| AATCTTTCAAACCTACCACA+TGG | - | chr6.2:14993706-14993725 | MS.gene074199:CDS | 35.0% | |
| ACGTAACATTAGCATCAACT+CGG | + | chr6.2:14993555-14993574 | None:intergenic | 35.0% | |
| ACTATAACACAACAAGTGAC+AGG | - | chr6.2:14992993-14993012 | MS.gene074199:CDS | 35.0% | |
| AGCTCATAAGTTCAACTACA+TGG | - | chr6.2:14993768-14993787 | MS.gene074199:CDS | 35.0% | |
| ATCTTACAATTCTCTTGGAC+CGG | - | chr6.2:14993132-14993151 | MS.gene074199:CDS | 35.0% | |
| ATGAGATTGAAGTAGCTACT+TGG | + | chr6.2:14993313-14993332 | None:intergenic | 35.0% | |
| ATTTGTAGGTACTCCTAACT+TGG | - | chr6.2:14993279-14993298 | MS.gene074199:CDS | 35.0% | |
| CAAAGGATGCATATGCTAAA+AGG | - | chr6.2:14993208-14993227 | MS.gene074199:CDS | 35.0% | |
| CATAAGTTCAACTACATGGA+TGG | - | chr6.2:14993772-14993791 | MS.gene074199:CDS | 35.0% | |
| CCACATGGATTTCAACTTAA+TGG | - | chr6.2:14993721-14993740 | MS.gene074199:CDS | 35.0% | |
| GAAAATCGACATGAAAAGGA+AGG | - | chr6.2:14993582-14993601 | MS.gene074199:CDS | 35.0% | |
| GCAAGCAGATTGATGTTAAT+TGG | - | chr6.2:14993515-14993534 | MS.gene074199:CDS | 35.0% | |
| GCATTGGAAATCATAGAAGA+AGG | + | chr6.2:14993439-14993458 | None:intergenic | 35.0% | |
| TAAAGATGATGTATCCGTCT+TGG | - | chr6.2:14993104-14993123 | MS.gene074199:CDS | 35.0% | |
| TAACATCAATCTGCTTGCAA+GGG | + | chr6.2:14993513-14993532 | None:intergenic | 35.0% | |
| TTAACATCAATCTGCTTGCA+AGG | + | chr6.2:14993514-14993533 | None:intergenic | 35.0% | |
| TTGTAAGATTCATCCCAAGA+CGG | + | chr6.2:14993121-14993140 | None:intergenic | 35.0% | |
| TTGTCGCCATACTATTACAA+TGG | - | chr6.2:14993628-14993647 | MS.gene074199:CDS | 35.0% | |
| ! | AGGTTTGAAAGATTCTGGAA+AGG | + | chr6.2:14993700-14993719 | None:intergenic | 35.0% |
| ! | ATAAAACCCCATTAGGGTTT+TGG | - | chr6.2:14993386-14993405 | MS.gene074199:CDS | 35.0% |
| ! | ATAGTATGAGCTTTGTGCAT+TGG | + | chr6.2:14993455-14993474 | None:intergenic | 35.0% |
| ! | TAAGTTGAAATCCATGTGGT+AGG | + | chr6.2:14993720-14993739 | None:intergenic | 35.0% |
| ! | TGTACCTCTCTTTTGTCTTT+AGG | + | chr6.2:14992956-14992975 | None:intergenic | 35.0% |
| !! | CCATTAAGTTGAAATCCATG+TGG | + | chr6.2:14993724-14993743 | None:intergenic | 35.0% |
| AACATTAGCATCAACTCGGT+TGG | + | chr6.2:14993551-14993570 | None:intergenic | 40.0% | |
| AAGTTGCCAAAACCCTAATG+GGG | + | chr6.2:14993395-14993414 | None:intergenic | 40.0% | |
| AGTTCAACTACATGGATGGA+AGG | - | chr6.2:14993776-14993795 | MS.gene074199:CDS | 40.0% | |
| ATGGCCTAAAGACAAAAGAG+AGG | - | chr6.2:14992949-14992968 | MS.gene074199:CDS | 40.0% | |
| GAAGTTGCCAAAACCCTAAT+GGG | + | chr6.2:14993396-14993415 | None:intergenic | 40.0% | |
| GCAAATGACCTCAATTGTTC+CGG | + | chr6.2:14993154-14993173 | None:intergenic | 40.0% | |
| GCTCCATAGATATTCTTCCA+CGG | + | chr6.2:14992772-14992791 | None:intergenic | 40.0% | |
| GTGGTAGGTTTGAAAGATTC+TGG | + | chr6.2:14993705-14993724 | None:intergenic | 40.0% | |
| TATCTTCTCTTTGCGCACTT+TGG | + | chr6.2:14993086-14993105 | None:intergenic | 40.0% | |
| TGAAGTTGCCAAAACCCTAA+TGG | + | chr6.2:14993397-14993416 | None:intergenic | 40.0% | |
| TGCACAAAGCTCATACTATC+CGG | - | chr6.2:14993456-14993475 | MS.gene074199:CDS | 40.0% | |
| TTGATGTTAATTGGGCCAAC+TGG | - | chr6.2:14993524-14993543 | MS.gene074199:CDS | 40.0% | |
| TTGTCTTTAGGCCATGTTTC+CGG | + | chr6.2:14992944-14992963 | None:intergenic | 40.0% | |
| GCTTGCAAGGGTAAGATTGT+GGG | + | chr6.2:14993501-14993520 | None:intergenic | 45.0% | |
| GGAAGAGAATGCATAGCAAC+AGG | + | chr6.2:14993679-14993698 | None:intergenic | 45.0% | |
| TACCTACCTTCTTGAGACTC+CGG | + | chr6.2:14993478-14993497 | None:intergenic | 45.0% | |
| TACTATCCGGAGTCTCAAGA+AGG | - | chr6.2:14993469-14993488 | MS.gene074199:CDS | 45.0% | |
| TGCTTGCAAGGGTAAGATTG+TGG | + | chr6.2:14993502-14993521 | None:intergenic | 45.0% | |
| ! | GAGGTCATTTGCTAGCATGT+TGG | - | chr6.2:14993162-14993181 | MS.gene074199:CDS | 45.0% |
| !! | AGGGTTTTGGCAACTTCAAC+TGG | - | chr6.2:14993399-14993418 | MS.gene074199:CDS | 45.0% |
| !! | GAAGTAGCTACTTGGCTTTG+AGG | + | chr6.2:14993305-14993324 | None:intergenic | 45.0% |
| !! | GATTTGCTGAACCGGAAACA+TGG | - | chr6.2:14992930-14992949 | MS.gene074199:CDS | 45.0% |
| !! | GGGTTTTGGCAACTTCAACT+GGG | - | chr6.2:14993400-14993419 | MS.gene074199:CDS | 45.0% |
| !! | TTCTCCTAACTTTGAAGCCC+CGG | - | chr6.2:14992907-14992926 | MS.gene074199:CDS | 45.0% |
| AATCCCGGGGCTTCAAAGTT+AGG | + | chr6.2:14992914-14992933 | None:intergenic | 50.0% | |
| ATCCGGAGTCTCAAGAAGGT+AGG | - | chr6.2:14993473-14993492 | MS.gene074199:CDS | 50.0% | |
| CTCTTGGACCGGAACAATTG+AGG | - | chr6.2:14993143-14993162 | MS.gene074199:CDS | 50.0% | |
| GGGCCGTGGAAGAATATCTA+TGG | - | chr6.2:14992766-14992785 | MS.gene074199:CDS | 50.0% | |
| GTTTCCGGTTCAGCAAATCC+CGG | + | chr6.2:14992929-14992948 | None:intergenic | 50.0% | |
| TTTCCGGTTCAGCAAATCCC+GGG | + | chr6.2:14992928-14992947 | None:intergenic | 50.0% | |
| ! | ATCAACTCGGTTGGTCCAGT+TGG | + | chr6.2:14993542-14993561 | None:intergenic | 50.0% |
| !! | CTTGGCTTTGAGGCCAAGTT+AGG | + | chr6.2:14993295-14993314 | None:intergenic | 50.0% |
| !! | TCTCCTAACTTTGAAGCCCC+GGG | - | chr6.2:14992908-14992927 | MS.gene074199:CDS | 50.0% |
| TTCCGGTTCAGCAAATCCCG+GGG | + | chr6.2:14992927-14992946 | None:intergenic | 55.0% | |
| !! | AGCCCCGGGATTTGCTGAAC+CGG | - | chr6.2:14992922-14992941 | MS.gene074199:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.2 | gene | 14992764 | 14993804 | 14992764 | ID=MS.gene074199 |
| chr6.2 | mRNA | 14992764 | 14993804 | 14992764 | ID=MS.gene074199.t1;Parent=MS.gene074199 |
| chr6.2 | exon | 14992764 | 14993804 | 14992764 | ID=MS.gene074199.t1.exon1;Parent=MS.gene074199.t1 |
| chr6.2 | CDS | 14992764 | 14993804 | 14992764 | ID=cds.MS.gene074199.t1;Parent=MS.gene074199.t1 |
| Gene Sequence |
| Protein sequence |
