AlfalfaGEDB | MS.gene074344

M. sativa cultivar XinJiangDaYe / MS.gene074344

KEGG: -
GO: -
PFAM: PF03195.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074344.t1	PNX93285.1	36	125	74	4	7	130	5	124	2.50E-09	72.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074344.t1	Q9SA51	30.8	107	70	2	12	116	15	119	1.2e-07	58.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074344.t1	A0A2K3MR59	36.0	125	74	4	7	130	5	124	1.8e-09	72.4

TFs/TRs:

Gene ID	Type	Classification
MS.gene074344.t1	TF	LOB

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074344.t1	MTR_4g083680	30.714	140	88	5	12	149	16	148	1.42e-14	68.6
MS.gene074344.t1	MTR_6g027710	33.945	109	66	3	12	117	41	146	3.53e-14	68.6
MS.gene074344.t1	MTR_2g094730	29.703	101	70	1	12	112	3	102	9.72e-13	63.5
MS.gene074344.t1	MTR_4g105170	32.857	140	79	5	12	146	12	141	1.32e-11	60.8
MS.gene074344.t1	MTR_3g073690	31.429	105	67	3	12	114	10	111	2.40e-11	60.5
MS.gene074344.t1	MTR_3g031660	31.148	122	72	4	12	131	8	119	3.69e-11	59.3
MS.gene074344.t1	MTR_3g071590	31.092	119	70	4	12	128	9	117	5.88e-11	58.5

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene074344.t1	AT1G16530	30.841	107	70	2	12	116	15	119	2.62e-12	62.4
MS.gene074344.t1	AT1G06280	32.990	97	60	3	12	106	25	118	7.76e-12	61.6
MS.gene074344.t1	AT5G63090	31.469	143	79	5	12	145	12	144	4.72e-11	59.3
MS.gene074344.t1	AT5G63090	31.469	143	79	5	12	145	12	144	4.72e-11	59.3
MS.gene074344.t1	AT5G63090	31.469	143	79	5	12	145	12	144	4.72e-11	59.3
MS.gene074344.t1	AT5G63090	31.469	143	79	5	12	145	12	144	4.72e-11	59.3

Find 69 sgRNAs with CRISPR-Local

Find 75 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGACTCCACAGTTGGGAAAT+AGG	0.252755	2.2:-6975481	None:intergenic
TCTTTGTTTGGATCATGATT+AGG	0.270821	2.2:+6974947	None:intergenic
CTTTGTTTGGATCATGATTA+GGG	0.280665	2.2:+6974948	None:intergenic
GAAGGAGTTAATAGATTATA+CGG	0.299232	2.2:+6975507	MS.gene074344:CDS
GGCCCTTGTGCAGCATTGGC+TGG	0.304155	2.2:-6975858	None:intergenic
GGCCCTTGTGCAGCATTGGC+TGG	0.304155	2.2:-6975888	None:intergenic
GGTTCTGCAGGATATCTATT+TGG	0.304685	2.2:-6975677	None:intergenic
CGTTGGCGAATGCTCTTAGA+TGG	0.306790	2.2:+6975589	MS.gene074344:CDS
GGCCCTTGTGCAGCAATGGC+TGG	0.329174	2.2:-6975828	None:intergenic
TGGTCCCGGGCCTCCATATA+TGG	0.351739	2.2:-6975657	None:intergenic
TGGCGGCCCTTGTGCAGCAT+TGG	0.359275	2.2:-6975862	None:intergenic
TGGCGGCCCTTGTGCAGCAT+TGG	0.359275	2.2:-6975892	None:intergenic
GGCCCTTGTGCAGCATCGGC+TGG	0.377247	2.2:-6975798	None:intergenic
GGCCCTTGTGCAGCAGCATT+AGG	0.384624	2.2:-6975768	None:intergenic
GACTCCACAGTTGGGAAATA+GGG	0.390044	2.2:-6975480	None:intergenic
CAGCCAGCCAATGCTGCACA+AGG	0.409089	2.2:+6975885	MS.gene074344:CDS
TAATCTATTAACTCCTTCAT+AGG	0.411601	2.2:-6975502	None:intergenic
AGGGTCGTACCGTCCATATA+TGG	0.421506	2.2:+6975644	MS.gene074344:CDS
GTTGTGGATGTTGCAAAGTT+AGG	0.421564	2.2:+6975421	MS.gene074344:CDS
TGGTGGCCCTTGTGCAGCAA+TGG	0.422296	2.2:-6975832	None:intergenic
AGGATCTGCATGCCTGTTGT+TGG	0.424351	2.2:-6975616	None:intergenic
ATTCGTTAACTTGGTTCTGC+AGG	0.436549	2.2:-6975689	None:intergenic
GCAGGATATCTATTTGGTCC+CGG	0.451870	2.2:-6975671	None:intergenic
TGTGCAGCATTGGCTGGCTG+TGG	0.468843	2.2:-6975852	None:intergenic
AGGAGGGCAAAGTGTGTTCA+AGG	0.471420	2.2:+6975441	MS.gene074344:CDS
TGTGCAGCAATGGCTGGCTG+TGG	0.481082	2.2:-6975822	None:intergenic
TGTGCAGCATCGGCTGGCTG+TGG	0.483226	2.2:-6975792	None:intergenic
CAGCCAGCCATTGCTGCACA+AGG	0.484315	2.2:+6975825	MS.gene074344:CDS
TTAGATGGGAAGCCAACAAC+AGG	0.485289	2.2:+6975604	MS.gene074344:CDS
GCTGTTGGTATTCGTTAACT+TGG	0.487276	2.2:-6975698	None:intergenic
CAGCCAGCCAATGCTGCACA+AGG	0.493671	2.2:+6975855	MS.gene074344:CDS
TGTGCAGCATTGGCTGGCTG+TGG	0.496961	2.2:-6975882	None:intergenic
TTGAAAAGATCAAGAAGCTC+TGG	0.500173	2.2:-6975912	None:intergenic
GCAGCAATGGCTGGCTGTGG+TGG	0.502838	2.2:-6975819	None:intergenic
CAACCTAATGCTGCTGCACA+AGG	0.508149	2.2:+6975765	MS.gene074344:CDS
CAGCCAGCCGATGCTGCACA+AGG	0.513999	2.2:+6975795	MS.gene074344:CDS
GCAGCATTGGCTGGCTGTGG+TGG	0.514659	2.2:-6975849	None:intergenic
TGGTGGCCCTTGTGCAGCAT+CGG	0.514892	2.2:-6975802	None:intergenic
ATACGGTTTCAAACGAGTAC+AGG	0.519560	2.2:+6975524	MS.gene074344:CDS
GTGGATGTTGCAAAGTTAGG+AGG	0.529022	2.2:+6975424	MS.gene074344:CDS
CCAACTGTGGAGTCCTATGA+AGG	0.531625	2.2:+6975489	MS.gene074344:CDS
CCTTCATAGGACTCCACAGT+TGG	0.532239	2.2:-6975489	None:intergenic
CAGGATATCTATTTGGTCCC+GGG	0.536492	2.2:-6975670	None:intergenic
TATATGGACGGTACGACCCT+CGG	0.540592	2.2:-6975641	None:intergenic
CTTCATAGGACTCCACAGTT+GGG	0.540765	2.2:-6975488	None:intergenic
ACCGTCCATATATGGAGGCC+CGG	0.542315	2.2:+6975652	MS.gene074344:CDS
GCAGCATCGGCTGGCTGTGG+TGG	0.555359	2.2:-6975789	None:intergenic
GCAGCATTGGCTGGCTGTGG+CGG	0.556701	2.2:-6975879	None:intergenic
TGCTCCCTATTTCCCAACTG+TGG	0.562996	2.2:+6975476	MS.gene074344:CDS
GGACGGTACGACCCTCGGAT+AGG	0.563969	2.2:-6975636	None:intergenic
AGATCAGCAGAGGCATTCGT+TGG	0.587527	2.2:+6975572	MS.gene074344:CDS
AACAGCAACTGCATACGCTT+AGG	0.590820	2.2:+6975715	MS.gene074344:CDS
CCGTCCATATATGGAGGCCC+GGG	0.593932	2.2:+6975653	MS.gene074344:CDS
GTTGGCGAATGCTCTTAGAT+GGG	0.596339	2.2:+6975590	MS.gene074344:CDS
GTTTCAAACGAGTACAGGAG+TGG	0.609853	2.2:+6975529	MS.gene074344:CDS
TAAGCGTATGCAGTTGCTGT+TGG	0.610952	2.2:-6975713	None:intergenic
TGGATGTTGCAAAGTTAGGA+GGG	0.616574	2.2:+6975425	MS.gene074344:CDS
TCTGCAGACATGGTGCGTTG+TGG	0.620868	2.2:+6975405	MS.gene074344:intron
CCCGGGCCTCCATATATGGA+CGG	0.622205	2.2:-6975653	None:intergenic
AGCCAGCCGATGCTGCACAA+GGG	0.624227	2.2:+6975796	MS.gene074344:CDS
AGCCAGCCATTGCTGCACAA+GGG	0.629628	2.2:+6975826	MS.gene074344:CDS
AGCCAGCCAATGCTGCACAA+GGG	0.635294	2.2:+6975856	MS.gene074344:CDS
AGCCAGCCAATGCTGCACAA+GGG	0.635294	2.2:+6975886	MS.gene074344:CDS
AAAAGATCAAGAAGCTCTGG+CGG	0.635639	2.2:-6975909	None:intergenic
GGCATGCAGATCCTATCCGA+GGG	0.646719	2.2:+6975625	MS.gene074344:CDS
AACCTAATGCTGCTGCACAA+GGG	0.655555	2.2:+6975766	MS.gene074344:CDS
AGGCATGCAGATCCTATCCG+AGG	0.661960	2.2:+6975624	MS.gene074344:CDS
GTGCACAAACAGATCAGCAG+AGG	0.666466	2.2:+6975562	MS.gene074344:CDS
GTCGTACCGTCCATATATGG+AGG	0.680882	2.2:+6975647	MS.gene074344:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CCAAACAAAATAAAATGAAA+TGG	-	chr2.2:6975045-6975064	None:intergenic	20.0%
!!!	CCATTTCATTTTATTTTGTT+TGG	+	chr2.2:6975042-6975061	MS.gene074344:intron	20.0%
!!!	TGATTTTTATTTCTGTGAAT+TGG	+	chr2.2:6975011-6975030	MS.gene074344:intron	20.0%
!	AAGATTCTATCTAATAACAC+AGG	-	chr2.2:6975328-6975347	None:intergenic	25.0%
!	GAAGGAGTTAATAGATTATA+CGG	+	chr2.2:6975507-6975526	MS.gene074344:CDS	25.0%
!	GTTTGGATCATGATTAATTA+GGG	+	chr2.2:6975059-6975078	MS.gene074344:intron	25.0%
!	TAATCTATTAACTCCTTCAT+AGG	-	chr2.2:6975505-6975524	None:intergenic	25.0%
!	TCACAGAAATAAAAATCACT+AGG	-	chr2.2:6975009-6975028	None:intergenic	25.0%
!	TGTTTGGATCATGATTAATT+AGG	+	chr2.2:6975058-6975077	MS.gene074344:intron	25.0%
!!	GTGTTATTAGATAGAATCTT+TGG	+	chr2.2:6975328-6975347	MS.gene074344:intron	25.0%
!!!	TTTTGTTTGGATCATGATTA+CGG	+	chr2.2:6975055-6975074	MS.gene074344:intron	25.0%
!!!	TAGGGTCATGATTAGTTTTA+AGG	+	chr2.2:6975077-6975096	MS.gene074344:intron	30.0%
	TAGGGTCAGAATTAGTAGTA+AGG	+	chr2.2:6974966-6974985	MS.gene074344:intron	35.0%
	TGTTTGTTTATCTGCAGACA+TGG	+	chr2.2:6975395-6975414	MS.gene074344:intron	35.0%
	TTGAAAAGATCAAGAAGCTC+TGG	-	chr2.2:6975915-6975934	None:intergenic	35.0%
!!!	GGTCATGATTAGTTTTAAGG+TGG	+	chr2.2:6975080-6975099	MS.gene074344:intron	35.0%
	ATACGGTTTCAAACGAGTAC+AGG	+	chr2.2:6975524-6975543	MS.gene074344:CDS	40.0%
	GCTGTTGGTATTCGTTAACT+TGG	-	chr2.2:6975701-6975720	None:intergenic	40.0%
	GGTTCTGCAGGATATCTATT+TGG	-	chr2.2:6975680-6975699	None:intergenic	40.0%
	GTTGTGGATGTTGCAAAGTT+AGG	+	chr2.2:6975421-6975440	MS.gene074344:CDS	40.0%
	TGGATGTTGCAAAGTTAGGA+GGG	+	chr2.2:6975425-6975444	MS.gene074344:CDS	40.0%
!	AAAAGATCAAGAAGCTCTGG+CGG	-	chr2.2:6975912-6975931	None:intergenic	40.0%
!!	ATTCGTTAACTTGGTTCTGC+AGG	-	chr2.2:6975692-6975711	None:intergenic	40.0%
	AACAGCAACTGCATACGCTT+AGG	+	chr2.2:6975715-6975734	MS.gene074344:CDS	45.0%
	AACCTAATGCTGCTGCACAA+GGG	+	chr2.2:6975766-6975785	MS.gene074344:CDS	45.0%
	CAGGATATCTATTTGGTCCC+GGG	-	chr2.2:6975673-6975692	None:intergenic	45.0%
	CTTCATAGGACTCCACAGTT+GGG	-	chr2.2:6975491-6975510	None:intergenic	45.0%
	GACTCCACAGTTGGGAAATA+GGG	-	chr2.2:6975483-6975502	None:intergenic	45.0%
	GCAGGATATCTATTTGGTCC+CGG	-	chr2.2:6975674-6975693	None:intergenic	45.0%
	GTGGATGTTGCAAAGTTAGG+AGG	+	chr2.2:6975424-6975443	MS.gene074344:CDS	45.0%
	GTTGGCGAATGCTCTTAGAT+GGG	+	chr2.2:6975590-6975609	MS.gene074344:CDS	45.0%
	GTTTCAAACGAGTACAGGAG+TGG	+	chr2.2:6975529-6975548	MS.gene074344:CDS	45.0%
	TAAGCGTATGCAGTTGCTGT+TGG	-	chr2.2:6975716-6975735	None:intergenic	45.0%
	TTAGATGGGAAGCCAACAAC+AGG	+	chr2.2:6975604-6975623	MS.gene074344:CDS	45.0%
	AGGAGGGCAAAGTGTGTTCA+AGG	+	chr2.2:6975441-6975460	MS.gene074344:CDS	50.0%
	AGGATCTGCATGCCTGTTGT+TGG	-	chr2.2:6975619-6975638	None:intergenic	50.0%
	AGGGTCGTACCGTCCATATA+TGG	+	chr2.2:6975644-6975663	MS.gene074344:CDS	50.0%
	CAACCTAATGCTGCTGCACA+AGG	+	chr2.2:6975765-6975784	MS.gene074344:CDS	50.0%
	CCAACTGTGGAGTCCTATGA+AGG	+	chr2.2:6975489-6975508	MS.gene074344:CDS	50.0%
	CCTTCATAGGACTCCACAGT+TGG	-	chr2.2:6975492-6975511	None:intergenic	50.0%
	CGTTGGCGAATGCTCTTAGA+TGG	+	chr2.2:6975589-6975608	MS.gene074344:CDS	50.0%
	GGACTCCACAGTTGGGAAAT+AGG	-	chr2.2:6975484-6975503	None:intergenic	50.0%
	GTCGTACCGTCCATATATGG+AGG	+	chr2.2:6975647-6975666	MS.gene074344:CDS	50.0%
	GTGCACAAACAGATCAGCAG+AGG	+	chr2.2:6975562-6975581	MS.gene074344:CDS	50.0%
	TATATGGACGGTACGACCCT+CGG	-	chr2.2:6975644-6975663	None:intergenic	50.0%
	TGCTCCCTATTTCCCAACTG+TGG	+	chr2.2:6975476-6975495	MS.gene074344:CDS	50.0%
!!	AGATCAGCAGAGGCATTCGT+TGG	+	chr2.2:6975572-6975591	MS.gene074344:CDS	50.0%
	ACCGTCCATATATGGAGGCC+CGG	+	chr2.2:6975652-6975671	MS.gene074344:CDS	55.0%
	AGCCAGCCAATGCTGCACAA+GGG	+	chr2.2:6975856-6975875	MS.gene074344:CDS	55.0%
	AGCCAGCCATTGCTGCACAA+GGG	+	chr2.2:6975826-6975845	MS.gene074344:CDS	55.0%
	AGGCATGCAGATCCTATCCG+AGG	+	chr2.2:6975624-6975643	MS.gene074344:CDS	55.0%
	GGCATGCAGATCCTATCCGA+GGG	+	chr2.2:6975625-6975644	MS.gene074344:CDS	55.0%
	TCTGCAGACATGGTGCGTTG+TGG	+	chr2.2:6975405-6975424	MS.gene074344:intron	55.0%
	AGCCAGCCGATGCTGCACAA+GGG	+	chr2.2:6975796-6975815	MS.gene074344:CDS	60.0%
	CAGCCAGCCAATGCTGCACA+AGG	+	chr2.2:6975855-6975874	MS.gene074344:CDS	60.0%
	CAGCCAGCCATTGCTGCACA+AGG	+	chr2.2:6975825-6975844	MS.gene074344:CDS	60.0%
	CCCGGGCCTCCATATATGGA+CGG	-	chr2.2:6975656-6975675	None:intergenic	60.0%
	CCGTCCATATATGGAGGCCC+GGG	+	chr2.2:6975653-6975672	MS.gene074344:CDS	60.0%
	GGCCCTTGTGCAGCAGCATT+AGG	-	chr2.2:6975771-6975790	None:intergenic	60.0%
	TGGTCCCGGGCCTCCATATA+TGG	-	chr2.2:6975660-6975679	None:intergenic	60.0%
	TGGTGGCCCTTGTGCAGCAA+TGG	-	chr2.2:6975835-6975854	None:intergenic	60.0%
	TGGTGGCCCTTGTGCAGCAT+CGG	-	chr2.2:6975805-6975824	None:intergenic	60.0%
!	TGTGCAGCAATGGCTGGCTG+TGG	-	chr2.2:6975825-6975844	None:intergenic	60.0%
!!	TGTGCAGCATTGGCTGGCTG+TGG	-	chr2.2:6975855-6975874	None:intergenic	60.0%
	CAGCCAGCCGATGCTGCACA+AGG	+	chr2.2:6975795-6975814	MS.gene074344:CDS	65.0%
	GGACGGTACGACCCTCGGAT+AGG	-	chr2.2:6975639-6975658	None:intergenic	65.0%
	GGCCCTTGTGCAGCAATGGC+TGG	-	chr2.2:6975831-6975850	None:intergenic	65.0%
	TGGCGGCCCTTGTGCAGCAT+TGG	-	chr2.2:6975865-6975884	None:intergenic	65.0%
!	GCAGCAATGGCTGGCTGTGG+TGG	-	chr2.2:6975822-6975841	None:intergenic	65.0%
!!	GCAGCATTGGCTGGCTGTGG+CGG	-	chr2.2:6975852-6975871	None:intergenic	65.0%
!!	GCAGCATTGGCTGGCTGTGG+TGG	-	chr2.2:6975852-6975871	None:intergenic	65.0%
!!	GGCCCTTGTGCAGCATTGGC+TGG	-	chr2.2:6975861-6975880	None:intergenic	65.0%
!!	TGTGCAGCATCGGCTGGCTG+TGG	-	chr2.2:6975795-6975814	None:intergenic	65.0%
!!	GCAGCATCGGCTGGCTGTGG+TGG	-	chr2.2:6975792-6975811	None:intergenic	70.0%
!!	GGCCCTTGTGCAGCATCGGC+TGG	-	chr2.2:6975801-6975820	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr2.2	gene	6974961	6975941	6974961	ID=MS.gene074344
chr2.2	mRNA	6974961	6975941	6974961	ID=MS.gene074344.t1;Parent=MS.gene074344
chr2.2	exon	6974961	6974982	6974961	ID=MS.gene074344.t1.exon1;Parent=MS.gene074344.t1
chr2.2	CDS	6974961	6974982	6974961	ID=cds.MS.gene074344.t1;Parent=MS.gene074344.t1
chr2.2	exon	6975412	6975941	6975412	ID=MS.gene074344.t1.exon2;Parent=MS.gene074344.t1
chr2.2	CDS	6975412	6975941	6975412	ID=cds.MS.gene074344.t1;Parent=MS.gene074344.t1

Gene Sequence

>MS.gene074344

ATGATTAGGGTCAGAATTAGTAGTAAGGTAGTTATTATTTACATGCCTAGTGATTTTTATTTCTGTGAATTGGATTTTTTACCATTTCATTTTATTTTGTTTGGATCATGATTAATTAGGGTCATGATTAGTTTTAAGGTGGTTATTATTTACATGCCTAGTGATTTTTATTTCTGTGAATTGAATTTTTTACCATTTCATTTTATTTTGTTTGGATCATGATTAATTAGGGTCATGATTAGTTTTAAGGTGGTTATTATTTACATGCCTAGTGATTTTTATTTCTGTGAATTGGATTTTTTACCATTTCATTTTATTTTGTTTGGATCATGATTACGGTGATAATTAACGAATTACTTTTGTACCTGTGTTATTAGATAGAATCTTTGGAATTCAATAATGTTCTATATGTTTGAATTTAATAAGCTGAATATTGTTTGTTTATCTGCAGACATGGTGCGTTGTGGATGTTGCAAAGTTAGGAGGGCAAAGTGTGTTCAAGGAGAATGCATCTATGCTCCCTATTTCCCAACTGTGGAGTCCTATGAAGGAGTTAATAGATTATACGGTTTCAAACGAGTACAGGAGTGGATTAACAATAGTGCACAAACAGATCAGCAGAGGCATTCGTTGGCGAATGCTCTTAGATGGGAAGCCAACAACAGGCATGCAGATCCTATCCGAGGGTCGTACCGTCCATATATGGAGGCCCGGGACCAAATAGATATCCTGCAGAACCAAGTTAACGAATACCAACAGCAACTGCATACGCTTAGGCTAAGAGTCGAACAATTGCAAGCACAGCAACCTAATGCTGCTGCACAAGGGCCACCACAGCCAGCCGATGCTGCACAAGGGCCACCACAGCCAGCCATTGCTGCACAAGGGCCACCACAGCCAGCCAATGCTGCACAAGGGCCGCCACAGCCAGCCAATGCTGCACAAGGGCCGCCAGAGCTTCTTGATCTTTTCAAATTTTAG

Protein sequence

>MS.gene074344.t1

MIRVRISNMVRCGCCKVRRAKCVQGECIYAPYFPTVESYEGVNRLYGFKRVQEWINNSAQTDQQRHSLANALRWEANNRHADPIRGSYRPYMEARDQIDILQNQVNEYQQQLHTLRLRVEQLQAQQPNAAAQGPPQPADAAQGPPQPAIAAQGPPQPANAAQGPPQPANAAQGPPELLDLFKF