AlfalfaGEDB | MS.gene07447

M. sativa cultivar XinJiangDaYe / MS.gene07447

KEGG: -
GO: GO:0003674 GO:0003676 GO:0003677 GO:0005488 GO:0097159 GO:1901363
PFAM: PF04770.15

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07447.t1	XP_013468809.1	92.7	192	13	1	8	199	1	191	4.10E-95	357.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07447.t1	Q9M9S0	49.5	198	88	5	10	195	84	281	4.9e-44	179.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07447.t1	G7L6E4	92.7	192	13	1	8	199	1	191	3.0e-95	357.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene07447.t1	TF	zf-HD

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07447.t1	MTR_1g077020	92.708	192	13	1	8	199	1	191	4.94e-131	366
MS.gene07447.t1	MTR_4g134580	41.772	237	83	5	15	196	112	348	1.56e-57	185
MS.gene07447.t1	MTR_5g032600	46.535	202	81	6	14	194	76	271	2.33e-53	172
MS.gene07447.t1	MTR_3g100650	46.231	199	88	4	13	194	77	273	1.02e-52	170
MS.gene07447.t1	MTR_5g020510	41.176	204	92	5	16	196	99	297	2.54e-47	157
MS.gene07447.t1	MTR_7g010300	41.414	198	100	3	13	194	85	282	2.88e-47	158
MS.gene07447.t1	MTR_5g065440	41.784	213	91	4	14	194	56	267	7.17e-47	155
MS.gene07447.t1	MTR_8g069080	38.500	200	103	4	15	194	68	267	9.15e-42	142
MS.gene07447.t1	MTR_2g076780	38.424	203	98	5	16	194	37	236	1.19e-41	140
MS.gene07447.t1	MTR_6g012280	40.090	222	62	7	16	194	91	284	1.42e-39	138
MS.gene07447.t1	MTR_6g073013	36.517	178	74	6	16	190	19	160	6.64e-34	119
MS.gene07447.t1	MTR_8g467340	63.492	63	23	0	132	194	271	333	8.06e-22	92.0
MS.gene07447.t1	MTR_8g467340	64.407	59	19	1	12	68	60	118	4.03e-19	84.3
MS.gene07447.t1	MTR_3g039810	62.264	53	20	0	14	66	18	70	6.92e-19	77.8
MS.gene07447.t1	MTR_8g069380	52.381	63	30	0	132	194	52	114	7.08e-17	73.6
MS.gene07447.t1	MTR_6g012220	60.000	55	18	1	10	64	15	65	7.17e-17	72.4
MS.gene07447.t1	MTR_8g442330	30.137	146	81	3	25	169	14	139	2.16e-16	72.8
MS.gene07447.t1	MTR_8g051480	53.226	62	28	1	133	194	68	128	7.71e-15	68.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene07447.t1	AT2G02540	47.867	211	79	4	9	194	80	284	2.17e-59	188
MS.gene07447.t1	AT2G02540	47.867	211	79	4	9	194	80	284	2.17e-59	188
MS.gene07447.t1	AT1G14440	49.495	198	88	5	10	195	84	281	6.01e-58	185
MS.gene07447.t1	AT1G14440	49.495	198	88	5	10	195	84	281	6.01e-58	185
MS.gene07447.t1	AT4G24660	44.737	190	79	3	14	194	47	219	8.80e-53	169
MS.gene07447.t1	AT4G24660	44.920	187	77	3	14	191	47	216	1.07e-51	166
MS.gene07447.t1	AT5G65410	43.386	189	87	2	15	193	74	252	1.51e-48	159
MS.gene07447.t1	AT1G69600	44.500	200	88	7	8	191	23	215	2.21e-48	158
MS.gene07447.t1	AT2G18350	41.935	186	102	2	14	196	80	262	3.97e-47	155
MS.gene07447.t1	AT5G60480	46.739	184	82	7	10	188	31	203	3.20e-46	152
MS.gene07447.t1	AT5G15210	44.211	190	93	5	9	188	49	235	5.43e-43	145
MS.gene07447.t1	AT3G50890	41.919	198	96	5	14	199	58	248	1.95e-42	143
MS.gene07447.t1	AT3G28920	40.930	215	77	5	14	191	50	251	1.68e-40	140
MS.gene07447.t1	AT5G42780	39.153	189	106	6	4	189	54	236	8.47e-29	107
MS.gene07447.t1	AT1G75240	69.643	56	17	0	11	66	71	126	2.01e-24	97.8
MS.gene07447.t1	AT1G75240	59.677	62	25	0	132	193	240	301	3.94e-20	86.3
MS.gene07447.t1	AT5G39760	57.576	66	28	0	132	197	200	265	5.22e-21	89.0
MS.gene07447.t1	AT1G74660	66.038	53	18	0	14	66	37	89	5.87e-21	83.6
MS.gene07447.t1	AT1G74660	66.038	53	18	0	14	66	37	89	7.25e-21	83.6
MS.gene07447.t1	AT3G28917	58.462	65	23	2	14	74	31	95	5.97e-20	80.9
MS.gene07447.t1	AT1G14687	28.889	180	105	6	16	191	7	167	2.03e-19	81.6
MS.gene07447.t1	AT1G18835	66.038	53	15	1	14	66	24	73	3.42e-18	76.3

Find 26 sgRNAs with CRISPR-Local

Find 32 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TAACATTAATGCACCCTTCT+TGG	0.256074	1.2:-63618125	MS.gene07447:CDS
TTATGATAAAGATTGTATTA+AGG	0.325403	1.2:-63618047	MS.gene07447:CDS
GATGTTGAGCTTTGCAGAAA+AGG	0.345023	1.2:-63617948	MS.gene07447:CDS
GCTTGTAGAAAGATTTCTCT+TGG	0.457726	1.2:+63617811	None:intergenic
AAGAACCATGCAGCTACAAT+TGG	0.462343	1.2:-63618325	MS.gene07447:CDS
TATTATGCATCCACACTTTG+AGG	0.473414	1.2:+63617849	None:intergenic
ATTGGTGGCAATGCAATTGA+TGG	0.483673	1.2:-63618307	MS.gene07447:CDS
TTATCTCCTAACAACAAAAG+TGG	0.483711	1.2:-63618097	MS.gene07447:CDS
AGGAGATAAATGAGCCAAGA+AGG	0.501984	1.2:+63618111	None:intergenic
TAAAGATTGTATTAAGGATG+TGG	0.506741	1.2:-63618041	MS.gene07447:CDS
GGTGCATTAATGTTATGATC+AGG	0.507932	1.2:+63618133	None:intergenic
TAAGTTCAGTAAAGAACAGA+AGG	0.524622	1.2:-63617975	MS.gene07447:CDS
AACCATGCAGCTACAATTGG+TGG	0.533193	1.2:-63618322	MS.gene07447:CDS
TTAATGTTATGATCAGGAAT+CGG	0.552299	1.2:+63618139	None:intergenic
AGAGCAGCATAATGTTGAGA+AGG	0.566633	1.2:+63618166	None:intergenic
TGCCACCAATTGTAGCTGCA+TGG	0.577086	1.2:+63618320	None:intergenic
AAAATATGATAATTAAGAAG+AGG	0.580153	1.2:-63618006	MS.gene07447:CDS
TCATAATAAGACTGATCAGA+AGG	0.589777	1.2:+63618064	None:intergenic
GGCAATGCAATTGATGGATG+TGG	0.602522	1.2:-63618301	MS.gene07447:CDS
TTGCAGAAAAGGCAGAGTGG+AGG	0.609267	1.2:-63617937	MS.gene07447:CDS
AGAGAAGAATCCTCAAAGTG+TGG	0.619701	1.2:-63617859	MS.gene07447:CDS
GAGCAGCATAATGTTGAGAA+GGG	0.623819	1.2:+63618167	None:intergenic
GGAGATAAATGAGCCAAGAA+GGG	0.630466	1.2:+63618112	None:intergenic
TCAGAAGCTAGATGAATCTG+TGG	0.653987	1.2:-63617912	MS.gene07447:CDS
GCTTTGCAGAAAAGGCAGAG+TGG	0.664958	1.2:-63617940	MS.gene07447:CDS
TGTGGTGAGTTCATGCCAAG+TGG	0.691258	1.2:-63618283	MS.gene07447:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAATATGATAATTAAGAAG+AGG	-	chr1.2:63618187-63618206	MS.gene07447:CDS	15.0%
!!	TTATGATAAAGATTGTATTA+AGG	-	chr1.2:63618146-63618165	MS.gene07447:CDS	15.0%
!!!	CTTCTTAATTATCATATTTT+CGG	+	chr1.2:63618187-63618206	None:intergenic	15.0%
!	TAAAGATTGTATTAAGGATG+TGG	-	chr1.2:63618152-63618171	MS.gene07447:CDS	25.0%
!	TTAATGTTATGATCAGGAAT+CGG	+	chr1.2:63618057-63618076	None:intergenic	25.0%
!!	ACTCTATAATTTCTTTTCTG+TGG	+	chr1.2:63617993-63618012	None:intergenic	25.0%
	TAAGTTCAGTAAAGAACAGA+AGG	-	chr1.2:63618218-63618237	MS.gene07447:CDS	30.0%
	TCATAATAAGACTGATCAGA+AGG	+	chr1.2:63618132-63618151	None:intergenic	30.0%
	TTATCTCCTAACAACAAAAG+TGG	-	chr1.2:63618096-63618115	MS.gene07447:CDS	30.0%
	GCTTGTAGAAAGATTTCTCT+TGG	+	chr1.2:63618385-63618404	None:intergenic	35.0%
	GGTGCATTAATGTTATGATC+AGG	+	chr1.2:63618063-63618082	None:intergenic	35.0%
	TAACATTAATGCACCCTTCT+TGG	-	chr1.2:63618068-63618087	MS.gene07447:CDS	35.0%
	TATTATGCATCCACACTTTG+AGG	+	chr1.2:63618347-63618366	None:intergenic	35.0%
!!	CAGAAGTTTTGTCAAGAGAT+TGG	-	chr1.2:63618306-63618325	MS.gene07447:CDS	35.0%
!!!	GTTGATCCACTTTTGTTGTT+AGG	+	chr1.2:63618105-63618124	None:intergenic	35.0%
	AAGAACCATGCAGCTACAAT+TGG	-	chr1.2:63617868-63617887	MS.gene07447:CDS	40.0%
	AGAGAAGAATCCTCAAAGTG+TGG	-	chr1.2:63618334-63618353	MS.gene07447:CDS	40.0%
	AGAGCAGCATAATGTTGAGA+AGG	+	chr1.2:63618030-63618049	None:intergenic	40.0%
	AGGAGATAAATGAGCCAAGA+AGG	+	chr1.2:63618085-63618104	None:intergenic	40.0%
	GAGCAGCATAATGTTGAGAA+GGG	+	chr1.2:63618029-63618048	None:intergenic	40.0%
	GATGTTGAGCTTTGCAGAAA+AGG	-	chr1.2:63618245-63618264	MS.gene07447:CDS	40.0%
	GGAGATAAATGAGCCAAGAA+GGG	+	chr1.2:63618084-63618103	None:intergenic	40.0%
	TCAGAAGCTAGATGAATCTG+TGG	-	chr1.2:63618281-63618300	MS.gene07447:CDS	40.0%
!	AGTGTGTCATGTTTTCCACT+TGG	+	chr1.2:63617928-63617947	None:intergenic	40.0%
!	ATTGGTGGCAATGCAATTGA+TGG	-	chr1.2:63617886-63617905	MS.gene07447:CDS	40.0%
!	CTTTTCTGTGGAAGTTTCTG+TGG	+	chr1.2:63617981-63618000	None:intergenic	40.0%
	AACCATGCAGCTACAATTGG+TGG	-	chr1.2:63617871-63617890	MS.gene07447:CDS	45.0%
!	GGCAATGCAATTGATGGATG+TGG	-	chr1.2:63617892-63617911	MS.gene07447:CDS	45.0%
	GCTTTGCAGAAAAGGCAGAG+TGG	-	chr1.2:63618253-63618272	MS.gene07447:CDS	50.0%
	TGCCACCAATTGTAGCTGCA+TGG	+	chr1.2:63617876-63617895	None:intergenic	50.0%
	TGTGGTGAGTTCATGCCAAG+TGG	-	chr1.2:63617910-63617929	MS.gene07447:CDS	50.0%
	TTGCAGAAAAGGCAGAGTGG+AGG	-	chr1.2:63618256-63618275	MS.gene07447:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.2	gene	63617808	63618407	63617808	ID=MS.gene07447
chr1.2	mRNA	63617808	63618407	63617808	ID=MS.gene07447.t1;Parent=MS.gene07447
chr1.2	exon	63617808	63618407	63617808	ID=MS.gene07447.t1.exon1;Parent=MS.gene07447.t1
chr1.2	CDS	63617808	63618407	63617808	ID=cds.MS.gene07447.t1;Parent=MS.gene07447.t1

Gene Sequence

>MS.gene07447

ATGGAGTCTAGCATTTTTCATGTTGAGAAAAAGATAGTAGTGAAATATAAAGAGTGTCTCAAGAACCATGCAGCTACAATTGGTGGCAATGCAATTGATGGATGTGGTGAGTTCATGCCAAGTGGAAAACATGACACACTTGAAGCTCTCAAATGTTGTGCTTGCAATTGCCACAGAAACTTCCACAGAAAAGAAATTATAGAGTCTGATTTCAATAGCCCTTCTCAACATTATGCTGCTCTTTCACCGATTCCTGATCATAACATTAATGCACCCTTCTTGGCTCATTTATCTCCTAACAACAAAAGTGGATCAACTAGTCCTTCTGATCAGTCTTATTATGATAAAGATTGTATTAAGGATGTGGAAAATCGAACCGAAAATATGATAATTAAGAAGAGGTCTAGAACTAAGTTCAGTAAAGAACAGAAGGAGAAGATGTTGAGCTTTGCAGAAAAGGCAGAGTGGAGGATTCAGAAGCTAGATGAATCTGTGGTGCAGAAGTTTTGTCAAGAGATTGGAATCAAGAGAAGAATCCTCAAAGTGTGGATGCATAATAACAAGAACACTTTTGCCAAGAGAAATCTTTCTACAAGCTAG

Protein sequence

>MS.gene07447.t1

MESSIFHVEKKIVVKYKECLKNHAATIGGNAIDGCGEFMPSGKHDTLEALKCCACNCHRNFHRKEIIESDFNSPSQHYAALSPIPDHNINAPFLAHLSPNNKSGSTSPSDQSYYDKDCIKDVENRTENMIIKKRSRTKFSKEQKEKMLSFAEKAEWRIQKLDESVVQKFCQEIGIKRRILKVWMHNNKNTFAKRNLSTS