Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074513.t1 | RHN50483.1 | 93.9 | 346 | 21 | 0 | 1 | 346 | 11 | 356 | 7.30E-188 | 666.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074513.t1 | Q9FIM0 | 27.6 | 275 | 176 | 8 | 6 | 261 | 6 | 276 | 2.6e-16 | 87.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074513.t1 | A0A072U6V5 | 93.9 | 346 | 21 | 0 | 1 | 346 | 1 | 346 | 5.2e-188 | 666.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene074513.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074513.t1 | MTR_6g018920 | 93.931 | 346 | 21 | 0 | 1 | 346 | 1 | 346 | 0.0 | 688 |
| MS.gene074513.t1 | MTR_1g114730 | 74.157 | 356 | 73 | 3 | 1 | 346 | 1 | 347 | 0.0 | 521 |
| MS.gene074513.t1 | MTR_6g005450 | 66.097 | 351 | 110 | 4 | 1 | 346 | 1 | 347 | 3.14e-160 | 452 |
| MS.gene074513.t1 | MTR_6g005440 | 61.850 | 346 | 101 | 3 | 1 | 346 | 1 | 315 | 1.70e-149 | 424 |
| MS.gene074513.t1 | MTR_4g107170 | 41.935 | 155 | 87 | 1 | 1 | 155 | 1 | 152 | 1.92e-37 | 135 |
| MS.gene074513.t1 | MTR_2g049610 | 43.226 | 155 | 82 | 1 | 1 | 155 | 1 | 149 | 3.36e-37 | 134 |
| MS.gene074513.t1 | MTR_4g045977 | 37.725 | 167 | 101 | 1 | 1 | 167 | 1 | 164 | 9.08e-34 | 129 |
| MS.gene074513.t1 | MTR_2g049530 | 37.662 | 154 | 91 | 2 | 3 | 155 | 5 | 154 | 1.22e-30 | 115 |
| MS.gene074513.t1 | MTR_2g049630 | 38.065 | 155 | 91 | 2 | 2 | 155 | 4 | 154 | 1.50e-28 | 114 |
| MS.gene074513.t1 | MTR_1g115290 | 32.231 | 121 | 81 | 1 | 44 | 164 | 8 | 127 | 5.67e-21 | 88.6 |
| MS.gene074513.t1 | MTR_0061s0010 | 76.744 | 43 | 10 | 0 | 29 | 71 | 1 | 43 | 7.39e-18 | 79.3 |
| MS.gene074513.t1 | MTR_0061s0010 | 71.795 | 39 | 11 | 0 | 131 | 169 | 42 | 80 | 8.15e-11 | 59.7 |
| MS.gene074513.t1 | MTR_3g466980 | 27.044 | 159 | 102 | 5 | 1 | 151 | 1 | 153 | 9.07e-16 | 75.9 |
| MS.gene074513.t1 | MTR_3g102570 | 29.936 | 157 | 94 | 4 | 1 | 157 | 1 | 141 | 1.57e-15 | 76.6 |
| MS.gene074513.t1 | MTR_3g031100 | 25.121 | 207 | 138 | 6 | 1 | 197 | 1 | 200 | 1.87e-15 | 75.5 |
| MS.gene074513.t1 | MTR_3g031240 | 31.746 | 126 | 79 | 3 | 1 | 125 | 1 | 120 | 3.58e-15 | 73.2 |
| MS.gene074513.t1 | MTR_5g075380 | 29.518 | 166 | 101 | 6 | 1 | 157 | 1 | 159 | 2.35e-14 | 70.5 |
| MS.gene074513.t1 | MTR_3g466890 | 32.099 | 162 | 92 | 7 | 3 | 154 | 2 | 155 | 3.25e-14 | 70.1 |
| MS.gene074513.t1 | MTR_3g065100 | 27.309 | 249 | 134 | 9 | 1 | 215 | 1 | 236 | 3.96e-14 | 71.6 |
| MS.gene074513.t1 | MTR_4g032620 | 37.607 | 117 | 58 | 5 | 1 | 116 | 1 | 103 | 4.59e-14 | 71.2 |
| MS.gene074513.t1 | MTR_4g063790 | 28.750 | 160 | 99 | 5 | 1 | 151 | 1 | 154 | 8.79e-14 | 68.9 |
| MS.gene074513.t1 | MTR_8g086290 | 31.293 | 147 | 81 | 4 | 2 | 136 | 12 | 150 | 4.31e-13 | 67.4 |
| MS.gene074513.t1 | MTR_3g466830 | 33.636 | 110 | 66 | 3 | 3 | 111 | 2 | 105 | 1.32e-12 | 65.5 |
| MS.gene074513.t1 | MTR_5g041650 | 29.907 | 107 | 68 | 2 | 1 | 106 | 38 | 138 | 1.87e-12 | 68.2 |
| MS.gene074513.t1 | MTR_5g021270 | 29.375 | 160 | 96 | 5 | 1 | 155 | 1 | 148 | 2.15e-12 | 66.2 |
| MS.gene074513.t1 | MTR_5g021270 | 29.375 | 160 | 96 | 5 | 1 | 155 | 1 | 148 | 2.22e-12 | 66.2 |
| MS.gene074513.t1 | MTR_7g075850 | 26.144 | 153 | 99 | 4 | 1 | 150 | 1 | 142 | 2.75e-12 | 66.2 |
| MS.gene074513.t1 | MTR_4g084740 | 26.415 | 159 | 106 | 2 | 1 | 159 | 1 | 148 | 5.01e-12 | 66.6 |
| MS.gene074513.t1 | MTR_8g079502 | 38.272 | 81 | 38 | 2 | 1 | 69 | 1 | 81 | 5.77e-12 | 63.9 |
| MS.gene074513.t1 | MTR_5g066960 | 46.552 | 58 | 31 | 0 | 1 | 58 | 1 | 58 | 5.81e-12 | 60.8 |
| MS.gene074513.t1 | MTR_8g087860 | 28.369 | 141 | 92 | 3 | 1 | 141 | 17 | 148 | 7.55e-12 | 65.1 |
| MS.gene074513.t1 | MTR_8g033270 | 26.667 | 150 | 101 | 3 | 1 | 150 | 1 | 141 | 1.09e-11 | 64.7 |
| MS.gene074513.t1 | MTR_2g016210 | 32.407 | 108 | 65 | 4 | 2 | 108 | 3 | 103 | 1.18e-11 | 64.3 |
| MS.gene074513.t1 | MTR_3g467080 | 26.220 | 164 | 106 | 7 | 1 | 155 | 1 | 158 | 1.25e-11 | 62.8 |
| MS.gene074513.t1 | MTR_2g017865 | 27.083 | 144 | 90 | 3 | 1 | 141 | 16 | 147 | 1.82e-11 | 63.9 |
| MS.gene074513.t1 | MTR_1g090710 | 25.455 | 165 | 103 | 5 | 1 | 154 | 1 | 156 | 2.25e-11 | 62.0 |
| MS.gene074513.t1 | MTR_4g131030 | 41.667 | 60 | 35 | 0 | 1 | 60 | 1 | 60 | 2.50e-11 | 62.8 |
| MS.gene074513.t1 | MTR_1g047550 | 41.667 | 60 | 35 | 0 | 1 | 60 | 1 | 60 | 2.59e-11 | 62.0 |
| MS.gene074513.t1 | MTR_1g053070 | 53.061 | 49 | 23 | 0 | 1 | 49 | 1 | 49 | 3.04e-11 | 63.2 |
| MS.gene074513.t1 | MTR_1g054265 | 43.333 | 60 | 34 | 0 | 1 | 60 | 1 | 60 | 3.61e-11 | 61.6 |
| MS.gene074513.t1 | MTR_1g029670 | 27.027 | 148 | 88 | 5 | 1 | 144 | 1 | 132 | 4.00e-11 | 61.6 |
| MS.gene074513.t1 | MTR_2g105290 | 26.429 | 140 | 99 | 1 | 1 | 136 | 11 | 150 | 4.02e-11 | 61.6 |
| MS.gene074513.t1 | MTR_5g047580 | 28.125 | 128 | 79 | 4 | 1 | 128 | 1 | 115 | 4.30e-11 | 60.8 |
| MS.gene074513.t1 | MTR_1g090697 | 26.087 | 161 | 106 | 5 | 1 | 154 | 1 | 155 | 4.43e-11 | 60.8 |
| MS.gene074513.t1 | MTR_1g090783 | 26.316 | 152 | 93 | 6 | 3 | 144 | 2 | 144 | 5.23e-11 | 60.8 |
| MS.gene074513.t1 | MTR_2g461710 | 46.939 | 49 | 26 | 0 | 1 | 49 | 1 | 49 | 6.12e-11 | 57.8 |
| MS.gene074513.t1 | MTR_3g088615 | 37.662 | 77 | 42 | 1 | 1 | 77 | 1 | 71 | 6.34e-11 | 61.2 |
| MS.gene074513.t1 | MTR_4g109830 | 28.022 | 182 | 99 | 6 | 1 | 168 | 1 | 164 | 6.65e-11 | 62.0 |
| MS.gene074513.t1 | MTR_2g009890 | 53.061 | 49 | 23 | 0 | 1 | 49 | 1 | 49 | 7.79e-11 | 62.4 |
| MS.gene074513.t1 | MTR_7g075870 | 27.273 | 154 | 96 | 4 | 1 | 150 | 1 | 142 | 8.56e-11 | 61.2 |
| MS.gene074513.t1 | MTR_6g015975 | 28.966 | 145 | 92 | 4 | 1 | 144 | 1 | 135 | 9.17e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074513.t1 | AT5G55690 | 39.073 | 151 | 84 | 2 | 1 | 150 | 1 | 144 | 2.45e-25 | 103 |
| MS.gene074513.t1 | AT5G55690 | 39.073 | 151 | 84 | 2 | 1 | 150 | 1 | 144 | 2.45e-25 | 103 |
| MS.gene074513.t1 | AT5G58890 | 36.301 | 146 | 90 | 2 | 6 | 150 | 6 | 149 | 2.32e-20 | 90.1 |
| MS.gene074513.t1 | AT3G18650 | 26.549 | 339 | 200 | 11 | 16 | 333 | 31 | 341 | 4.41e-18 | 84.7 |
| MS.gene074513.t1 | AT1G22590 | 31.056 | 161 | 100 | 3 | 1 | 156 | 1 | 155 | 2.25e-16 | 76.3 |
| MS.gene074513.t1 | AT1G65300 | 29.747 | 158 | 97 | 7 | 5 | 154 | 4 | 155 | 1.69e-14 | 73.2 |
| MS.gene074513.t1 | AT1G65330 | 34.234 | 111 | 66 | 4 | 3 | 112 | 2 | 106 | 9.14e-14 | 71.2 |
| MS.gene074513.t1 | AT5G27960 | 26.180 | 233 | 131 | 9 | 5 | 202 | 3 | 229 | 1.34e-13 | 71.2 |
| MS.gene074513.t1 | AT5G26650 | 32.110 | 109 | 67 | 3 | 5 | 112 | 3 | 105 | 3.25e-13 | 70.5 |
| MS.gene074513.t1 | AT5G23260 | 25.263 | 190 | 125 | 4 | 1 | 186 | 1 | 177 | 5.01e-13 | 68.2 |
| MS.gene074513.t1 | AT3G05860 | 38.961 | 77 | 41 | 3 | 1 | 77 | 1 | 71 | 9.62e-13 | 67.0 |
| MS.gene074513.t1 | AT3G05860 | 38.961 | 77 | 41 | 3 | 1 | 77 | 1 | 71 | 1.21e-12 | 67.4 |
| MS.gene074513.t1 | AT3G05860 | 38.961 | 77 | 41 | 3 | 1 | 77 | 1 | 71 | 1.37e-12 | 67.4 |
| MS.gene074513.t1 | AT5G23260 | 25.949 | 158 | 101 | 3 | 1 | 155 | 17 | 161 | 1.41e-12 | 67.4 |
| MS.gene074513.t1 | AT5G23260 | 26.144 | 153 | 97 | 3 | 1 | 150 | 1 | 140 | 1.50e-12 | 67.0 |
| MS.gene074513.t1 | AT4G11880 | 40.000 | 75 | 45 | 0 | 1 | 75 | 1 | 75 | 2.02e-12 | 65.5 |
| MS.gene074513.t1 | AT4G11880 | 40.000 | 75 | 45 | 0 | 1 | 75 | 1 | 75 | 2.02e-12 | 65.5 |
| MS.gene074513.t1 | AT5G23260 | 26.144 | 153 | 97 | 3 | 1 | 150 | 17 | 156 | 2.31e-12 | 67.0 |
| MS.gene074513.t1 | AT5G26580 | 49.153 | 59 | 30 | 0 | 1 | 59 | 1 | 59 | 4.27e-12 | 67.0 |
| MS.gene074513.t1 | AT3G57230 | 52.000 | 50 | 24 | 0 | 1 | 50 | 1 | 50 | 5.10e-12 | 63.5 |
| MS.gene074513.t1 | AT4G11880 | 40.000 | 75 | 45 | 0 | 1 | 75 | 1 | 75 | 5.67e-12 | 65.1 |
| MS.gene074513.t1 | AT4G11880 | 40.000 | 75 | 45 | 0 | 1 | 75 | 1 | 75 | 5.67e-12 | 65.1 |
| MS.gene074513.t1 | AT2G45650 | 30.464 | 151 | 93 | 5 | 1 | 150 | 1 | 140 | 7.91e-12 | 65.1 |
| MS.gene074513.t1 | AT5G62165 | 32.990 | 97 | 57 | 1 | 1 | 97 | 1 | 89 | 9.16e-12 | 61.6 |
| MS.gene074513.t1 | AT2G03060 | 28.972 | 107 | 69 | 2 | 1 | 106 | 1 | 101 | 9.52e-12 | 65.9 |
| MS.gene074513.t1 | AT2G03060 | 28.972 | 107 | 69 | 2 | 1 | 106 | 1 | 101 | 9.52e-12 | 65.9 |
| MS.gene074513.t1 | AT2G03060 | 28.972 | 107 | 69 | 2 | 1 | 106 | 1 | 101 | 9.52e-12 | 65.9 |
| MS.gene074513.t1 | AT5G62165 | 27.500 | 160 | 96 | 4 | 1 | 154 | 1 | 146 | 1.13e-11 | 62.8 |
| MS.gene074513.t1 | AT5G62165 | 27.500 | 160 | 96 | 4 | 1 | 154 | 1 | 146 | 1.13e-11 | 62.8 |
| MS.gene074513.t1 | AT5G62165 | 27.500 | 160 | 96 | 4 | 1 | 154 | 1 | 146 | 1.13e-11 | 62.8 |
| MS.gene074513.t1 | AT5G62165 | 27.500 | 160 | 96 | 4 | 1 | 154 | 1 | 146 | 1.13e-11 | 62.8 |
| MS.gene074513.t1 | AT5G62165 | 27.500 | 160 | 96 | 4 | 1 | 154 | 1 | 146 | 1.13e-11 | 62.8 |
| MS.gene074513.t1 | AT2G03060 | 28.972 | 107 | 69 | 2 | 1 | 106 | 1 | 101 | 1.13e-11 | 65.9 |
| MS.gene074513.t1 | AT3G57230 | 52.000 | 50 | 24 | 0 | 1 | 50 | 1 | 50 | 1.32e-11 | 64.3 |
| MS.gene074513.t1 | AT3G57230 | 52.000 | 50 | 24 | 0 | 1 | 50 | 1 | 50 | 1.32e-11 | 64.3 |
| MS.gene074513.t1 | AT2G34440 | 26.471 | 170 | 104 | 5 | 1 | 162 | 1 | 157 | 1.43e-11 | 62.8 |
| MS.gene074513.t1 | AT3G57390 | 40.244 | 82 | 41 | 1 | 1 | 82 | 1 | 74 | 1.48e-11 | 63.9 |
| MS.gene074513.t1 | AT1G18750 | 28.972 | 107 | 69 | 2 | 1 | 106 | 1 | 101 | 1.54e-11 | 65.5 |
| MS.gene074513.t1 | AT2G22630 | 46.552 | 58 | 31 | 0 | 1 | 58 | 1 | 58 | 1.59e-11 | 63.9 |
| MS.gene074513.t1 | AT2G22630 | 46.552 | 58 | 31 | 0 | 1 | 58 | 1 | 58 | 1.59e-11 | 63.9 |
| MS.gene074513.t1 | AT2G45660 | 27.957 | 186 | 111 | 6 | 1 | 176 | 1 | 173 | 1.74e-11 | 63.5 |
| MS.gene074513.t1 | AT1G18750 | 28.972 | 107 | 69 | 2 | 1 | 106 | 1 | 101 | 1.97e-11 | 65.1 |
| MS.gene074513.t1 | AT1G77980 | 22.642 | 159 | 112 | 2 | 1 | 159 | 1 | 148 | 2.05e-11 | 64.7 |
| MS.gene074513.t1 | AT1G18750 | 28.972 | 107 | 69 | 2 | 1 | 106 | 1 | 101 | 2.14e-11 | 65.1 |
| MS.gene074513.t1 | AT3G57390 | 40.244 | 82 | 41 | 1 | 1 | 82 | 1 | 74 | 2.52e-11 | 63.5 |
| MS.gene074513.t1 | AT3G54340 | 25.333 | 150 | 100 | 2 | 1 | 150 | 1 | 138 | 2.57e-11 | 63.2 |
| MS.gene074513.t1 | AT4G18960 | 25.342 | 146 | 94 | 4 | 2 | 144 | 18 | 151 | 2.60e-11 | 63.5 |
| MS.gene074513.t1 | AT5G62165 | 30.275 | 109 | 68 | 1 | 1 | 109 | 1 | 101 | 2.97e-11 | 61.6 |
| MS.gene074513.t1 | AT5G62165 | 30.275 | 109 | 68 | 1 | 1 | 109 | 1 | 101 | 2.97e-11 | 61.6 |
| MS.gene074513.t1 | AT5G62165 | 30.275 | 109 | 68 | 1 | 1 | 109 | 1 | 101 | 2.97e-11 | 61.6 |
| MS.gene074513.t1 | AT1G01530 | 24.153 | 236 | 151 | 7 | 1 | 218 | 6 | 231 | 3.00e-11 | 63.2 |
| MS.gene074513.t1 | AT2G28700 | 25.882 | 170 | 106 | 7 | 1 | 164 | 1 | 156 | 3.10e-11 | 64.3 |
| MS.gene074513.t1 | AT5G62165 | 30.275 | 109 | 68 | 1 | 1 | 109 | 1 | 101 | 3.14e-11 | 62.4 |
| MS.gene074513.t1 | AT5G62165 | 30.275 | 109 | 68 | 1 | 1 | 109 | 1 | 101 | 3.14e-11 | 62.4 |
| MS.gene074513.t1 | AT2G42830 | 26.380 | 163 | 104 | 5 | 1 | 159 | 16 | 166 | 3.35e-11 | 63.2 |
| MS.gene074513.t1 | AT2G45660 | 38.095 | 84 | 44 | 1 | 1 | 84 | 1 | 76 | 3.40e-11 | 61.2 |
| MS.gene074513.t1 | AT2G42830 | 26.380 | 163 | 104 | 5 | 1 | 159 | 16 | 166 | 4.02e-11 | 62.8 |
| MS.gene074513.t1 | AT1G22130 | 22.642 | 159 | 112 | 2 | 1 | 159 | 1 | 148 | 4.19e-11 | 63.9 |
| MS.gene074513.t1 | AT1G31640 | 28.829 | 111 | 71 | 3 | 3 | 112 | 2 | 105 | 4.46e-11 | 64.3 |
| MS.gene074513.t1 | AT5G62165 | 30.275 | 109 | 68 | 1 | 1 | 109 | 1 | 101 | 4.58e-11 | 62.0 |
| MS.gene074513.t1 | AT5G62165 | 30.275 | 109 | 68 | 1 | 1 | 109 | 1 | 101 | 4.58e-11 | 62.0 |
| MS.gene074513.t1 | AT5G62165 | 30.275 | 109 | 68 | 1 | 1 | 109 | 1 | 101 | 4.58e-11 | 62.0 |
| MS.gene074513.t1 | AT5G13790 | 51.020 | 49 | 24 | 0 | 1 | 49 | 1 | 49 | 4.81e-11 | 63.2 |
| MS.gene074513.t1 | AT4G09960 | 26.582 | 158 | 99 | 4 | 1 | 154 | 1 | 145 | 4.92e-11 | 62.4 |
| MS.gene074513.t1 | AT5G60910 | 28.758 | 153 | 95 | 4 | 1 | 150 | 1 | 142 | 4.94e-11 | 62.8 |
| MS.gene074513.t1 | AT4G09960 | 26.582 | 158 | 99 | 4 | 1 | 154 | 2 | 146 | 5.13e-11 | 62.4 |
| MS.gene074513.t1 | AT4G09960 | 26.582 | 158 | 99 | 4 | 1 | 154 | 11 | 155 | 5.26e-11 | 62.4 |
| MS.gene074513.t1 | AT4G11250 | 26.471 | 204 | 133 | 7 | 19 | 220 | 13 | 201 | 8.85e-11 | 62.8 |
Find 74 sgRNAs with CRISPR-Local
Find 79 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CCACATGGATTTCAACTTAA+TGG | 0.109578 | 6.1:-4879733 | MS.gene074513:CDS |
| GCAAGCAGATTGATGTTAAT+TGG | 0.174718 | 6.1:-4879939 | MS.gene074513:CDS |
| GTGGTAGGTTTGAAAGATTC+TGG | 0.248071 | 6.1:+4879752 | None:intergenic |
| TTGTCTTTAGGCCATGTTTC+CGG | 0.275357 | 6.1:+4880513 | None:intergenic |
| ATTAGTGATAAAACCCCATT+AGG | 0.276525 | 6.1:-4880075 | MS.gene074513:CDS |
| GCATTTCCATTGTAATAGTA+TGG | 0.277693 | 6.1:+4879820 | None:intergenic |
| TATGCTAAAAGGAGATATTA+AGG | 0.280393 | 6.1:-4880235 | MS.gene074513:CDS |
| ATAAAACCCCATTAGGGTTT+TGG | 0.280522 | 6.1:-4880068 | MS.gene074513:CDS |
| CAACGGATGCATATGCTAAA+AGG | 0.306043 | 6.1:-4880246 | MS.gene074513:CDS |
| CTTGGCTTTGAGGCCAAGTT+AGG | 0.322315 | 6.1:+4880162 | None:intergenic |
| GAAGTTGCCAAAACCCTAAT+GGG | 0.332063 | 6.1:+4880061 | None:intergenic |
| TCTTGAACATCATAGATCTT+AGG | 0.373714 | 6.1:+4880435 | None:intergenic |
| AATCCCGGGGCTTCAAAGTT+AGG | 0.381492 | 6.1:+4880543 | None:intergenic |
| CAAGCAGATTGATGTTAATT+GGG | 0.383614 | 6.1:-4879938 | MS.gene074513:CDS |
| TGAAGTTGCCAAAACCCTAA+TGG | 0.404084 | 6.1:+4880060 | None:intergenic |
| GATGAATCTTACAATTCTCT+TGG | 0.404689 | 6.1:-4880327 | MS.gene074513:CDS |
| TTGATGTTAATTGGGCCAAC+TGG | 0.408093 | 6.1:-4879930 | MS.gene074513:CDS |
| GGGCCGTGGAAGAATATCTA+TGG | 0.413157 | 6.1:-4880688 | MS.gene074513:CDS |
| AACATCAACATCACAAAGAA+TGG | 0.436469 | 6.1:+4880584 | None:intergenic |
| TTCTCCTAACTTTGAAGCCC+CGG | 0.437012 | 6.1:-4880547 | MS.gene074513:CDS |
| GGAAGAGAATGCATAGCAAC+AGG | 0.437355 | 6.1:+4879778 | None:intergenic |
| GCAAATGACCTCAATTGTTC+CGG | 0.442221 | 6.1:+4880303 | None:intergenic |
| CACAAGGTTGATAAATTTGT+AGG | 0.443122 | 6.1:-4880189 | MS.gene074513:CDS |
| ATCTGAAAATCGACATGAAA+AGG | 0.444904 | 6.1:-4879876 | MS.gene074513:CDS |
| AGCCCCGGGATTTGCTGAAC+CGG | 0.446619 | 6.1:-4880532 | MS.gene074513:CDS |
| ACATTTCAAAAGAGAAAGAA+TGG | 0.448242 | 6.1:-4880633 | MS.gene074513:CDS |
| GAAGTAGCTACTTGGCTTTG+AGG | 0.451246 | 6.1:+4880152 | None:intergenic |
| TTAGTGATAAAACCCCATTA+GGG | 0.454096 | 6.1:-4880074 | MS.gene074513:CDS |
| AAAGATGATGTATCCGTCTT+GGG | 0.455496 | 6.1:-4880349 | MS.gene074513:CDS |
| TATCTTCTCTTTGCGCACTT+TGG | 0.461986 | 6.1:+4880371 | None:intergenic |
| CTCTTGGACCGGAACAATTG+AGG | 0.477266 | 6.1:-4880311 | MS.gene074513:CDS |
| ATCTTACAATTCTCTTGGAC+CGG | 0.481367 | 6.1:-4880322 | MS.gene074513:CDS |
| TAAAGATGATGTATCCGTCT+TGG | 0.481458 | 6.1:-4880350 | MS.gene074513:CDS |
| TTTCCGGTTCAGCAAATCCC+GGG | 0.482987 | 6.1:+4880529 | None:intergenic |
| ATGAGATTGAAGTAGCTACT+TGG | 0.483532 | 6.1:+4880144 | None:intergenic |
| ATTTGTAGGTACTCCTAACT+TGG | 0.486423 | 6.1:-4880175 | MS.gene074513:CDS |
| TTATCACTAATATTCATCAA+TGG | 0.491553 | 6.1:+4880087 | None:intergenic |
| ATAGTATGAGCTTTGTGCAT+TGG | 0.491704 | 6.1:+4880002 | None:intergenic |
| TCTCCTAACTTTGAAGCCCC+GGG | 0.498039 | 6.1:-4880546 | MS.gene074513:CDS |
| ATGCTAAAAGGAGATATTAA+GGG | 0.498536 | 6.1:-4880234 | MS.gene074513:CDS |
| TTAACATCAATCTGCTTGCA+AGG | 0.499911 | 6.1:+4879943 | None:intergenic |
| GTTTCCGGTTCAGCAAATCC+CGG | 0.517428 | 6.1:+4880528 | None:intergenic |
| GCTTGCAAGGGTAAGATTGT+GGG | 0.518462 | 6.1:+4879956 | None:intergenic |
| TGCACAAAGCTCATACTATC+CGG | 0.522726 | 6.1:-4879998 | MS.gene074513:CDS |
| AGCTCATAAGTTCAACTACA+TGG | 0.526542 | 6.1:-4879686 | MS.gene074513:CDS |
| TGCTTGCAAGGGTAAGATTG+TGG | 0.530731 | 6.1:+4879955 | None:intergenic |
| ATCAACTCGGTTGGTCCAGT+TGG | 0.531175 | 6.1:+4879915 | None:intergenic |
| TTGTCGCCATACTATTACAA+TGG | 0.533945 | 6.1:-4879826 | MS.gene074513:CDS |
| CATAAGTTCAACTACATGGA+TGG | 0.541076 | 6.1:-4879682 | MS.gene074513:CDS |
| TAAGTTGAAATCCATGTGGT+AGG | 0.546009 | 6.1:+4879737 | None:intergenic |
| GCATTGGAAATCATAGAAGA+AGG | 0.559558 | 6.1:+4880018 | None:intergenic |
| GAGGTCATTTGCTAGCATGT+TGG | 0.565239 | 6.1:-4880292 | MS.gene074513:CDS |
| GATTTGCTGAACCGGAAACA+TGG | 0.565854 | 6.1:-4880524 | MS.gene074513:CDS |
| ACTATAACACAACAAGTGAC+AGG | 0.568787 | 6.1:-4880461 | MS.gene074513:CDS |
| GAAAATCGACATGAAAAGGA+AGG | 0.574406 | 6.1:-4879872 | MS.gene074513:CDS |
| TACCTACCTTCTTGAGACTC+CGG | 0.579791 | 6.1:+4879979 | None:intergenic |
| AACTTGATGCTTGTAATCAA+CGG | 0.581110 | 6.1:-4880263 | MS.gene074513:CDS |
| AGTTCAACTACATGGATGGA+AGG | 0.584745 | 6.1:-4879678 | MS.gene074513:CDS |
| TACTATCCGGAGTCTCAAGA+AGG | 0.586253 | 6.1:-4879985 | MS.gene074513:CDS |
| TCACTAATATTCATCAATGG+AGG | 0.597003 | 6.1:+4880090 | None:intergenic |
| TCCCCATAGAAAATGGGCCG+TGG | 0.598979 | 6.1:-4880702 | None:intergenic |
| TAACATCAATCTGCTTGCAA+GGG | 0.599195 | 6.1:+4879944 | None:intergenic |
| AACATTAGCATCAACTCGGT+TGG | 0.603209 | 6.1:+4879906 | None:intergenic |
| AGGTTTGAAAGATTCTGGAA+AGG | 0.625479 | 6.1:+4879757 | None:intergenic |
| AAGTTGCCAAAACCCTAATG+GGG | 0.636047 | 6.1:+4880062 | None:intergenic |
| AATCTTTCAAACCTACCACA+TGG | 0.651037 | 6.1:-4879748 | MS.gene074513:CDS |
| TTGTAAGATTCATCCCAAGA+CGG | 0.662155 | 6.1:+4880336 | None:intergenic |
| TTCCGGTTCAGCAAATCCCG+GGG | 0.665746 | 6.1:+4880530 | None:intergenic |
| ATCCGGAGTCTCAAGAAGGT+AGG | 0.678714 | 6.1:-4879981 | MS.gene074513:CDS |
| GCTCCATAGATATTCTTCCA+CGG | 0.693432 | 6.1:+4880685 | None:intergenic |
| ACGTAACATTAGCATCAACT+CGG | 0.701269 | 6.1:+4879902 | None:intergenic |
| AATAGCTCATGAATCACACA+AGG | 0.705760 | 6.1:-4880205 | MS.gene074513:CDS |
| ATGGCCTAAAGACAAAAGAG+AGG | 0.713382 | 6.1:-4880505 | MS.gene074513:CDS |
| CCATTAAGTTGAAATCCATG+TGG | 0.755933 | 6.1:+4879733 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATCACTAATATTCATCAA+TGG | + | chr6.1:4880278-4880297 | None:intergenic | 20.0% |
| ! | ACATTTCAAAAGAGAAAGAA+TGG | - | chr6.1:4879729-4879748 | MS.gene074513:CDS | 25.0% |
| ! | ATGCTAAAAGGAGATATTAA+GGG | - | chr6.1:4880128-4880147 | MS.gene074513:CDS | 25.0% |
| ! | TATGCTAAAAGGAGATATTA+AGG | - | chr6.1:4880127-4880146 | MS.gene074513:CDS | 25.0% |
| !! | TTGTGTTATAGTACTTTTGA+AGG | + | chr6.1:4879893-4879912 | None:intergenic | 25.0% |
| !!! | ATTCTTTCTCTTTTGAAATG+TGG | + | chr6.1:4879730-4879749 | None:intergenic | 25.0% |
| !!! | TGATGTTCAAGAGTATTTTA+AGG | - | chr6.1:4879938-4879957 | MS.gene074513:CDS | 25.0% |
| AACATCAACATCACAAAGAA+TGG | + | chr6.1:4879781-4879800 | None:intergenic | 30.0% | |
| AACTTGATGCTTGTAATCAA+CGG | - | chr6.1:4880099-4880118 | MS.gene074513:CDS | 30.0% | |
| ATCTGAAAATCGACATGAAA+AGG | - | chr6.1:4880486-4880505 | MS.gene074513:CDS | 30.0% | |
| ATTAGTGATAAAACCCCATT+AGG | - | chr6.1:4880287-4880306 | MS.gene074513:CDS | 30.0% | |
| CAAGCAGATTGATGTTAATT+GGG | - | chr6.1:4880424-4880443 | MS.gene074513:CDS | 30.0% | |
| GATGAATCTTACAATTCTCT+TGG | - | chr6.1:4880035-4880054 | MS.gene074513:CDS | 30.0% | |
| GCATTTCCATTGTAATAGTA+TGG | + | chr6.1:4880545-4880564 | None:intergenic | 30.0% | |
| TCACTAATATTCATCAATGG+AGG | + | chr6.1:4880275-4880294 | None:intergenic | 30.0% | |
| TCTTGAACATCATAGATCTT+AGG | + | chr6.1:4879930-4879949 | None:intergenic | 30.0% | |
| TTAGTGATAAAACCCCATTA+GGG | - | chr6.1:4880288-4880307 | MS.gene074513:CDS | 30.0% | |
| !! | CACAAGGTTGATAAATTTGT+AGG | - | chr6.1:4880173-4880192 | MS.gene074513:CDS | 30.0% |
| AAAGATGATGTATCCGTCTT+GGG | - | chr6.1:4880013-4880032 | MS.gene074513:CDS | 35.0% | |
| AATAGCTCATGAATCACACA+AGG | - | chr6.1:4880157-4880176 | MS.gene074513:CDS | 35.0% | |
| AATCTTTCAAACCTACCACA+TGG | - | chr6.1:4880614-4880633 | MS.gene074513:CDS | 35.0% | |
| ACGTAACATTAGCATCAACT+CGG | + | chr6.1:4880463-4880482 | None:intergenic | 35.0% | |
| ACTATAACACAACAAGTGAC+AGG | - | chr6.1:4879901-4879920 | MS.gene074513:CDS | 35.0% | |
| AGCTCATAAGTTCAACTACA+TGG | - | chr6.1:4880676-4880695 | MS.gene074513:CDS | 35.0% | |
| ATCTTACAATTCTCTTGGAC+CGG | - | chr6.1:4880040-4880059 | MS.gene074513:CDS | 35.0% | |
| ATGAGATTGAAGTAGCTACT+TGG | + | chr6.1:4880221-4880240 | None:intergenic | 35.0% | |
| ATTTGTAGGTACTCCTAACT+TGG | - | chr6.1:4880187-4880206 | MS.gene074513:CDS | 35.0% | |
| CATAAGTTCAACTACATGGA+TGG | - | chr6.1:4880680-4880699 | MS.gene074513:CDS | 35.0% | |
| CCACATGGATTTCAACTTAA+TGG | - | chr6.1:4880629-4880648 | MS.gene074513:CDS | 35.0% | |
| GAAAATCGACATGAAAAGGA+AGG | - | chr6.1:4880490-4880509 | MS.gene074513:CDS | 35.0% | |
| GCAAGCAGATTGATGTTAAT+TGG | - | chr6.1:4880423-4880442 | MS.gene074513:CDS | 35.0% | |
| GCATTGGAAATCATAGAAGA+AGG | + | chr6.1:4880347-4880366 | None:intergenic | 35.0% | |
| TAAAGATGATGTATCCGTCT+TGG | - | chr6.1:4880012-4880031 | MS.gene074513:CDS | 35.0% | |
| TAACATCAATCTGCTTGCAA+GGG | + | chr6.1:4880421-4880440 | None:intergenic | 35.0% | |
| TTAACATCAATCTGCTTGCA+AGG | + | chr6.1:4880422-4880441 | None:intergenic | 35.0% | |
| TTGTAAGATTCATCCCAAGA+CGG | + | chr6.1:4880029-4880048 | None:intergenic | 35.0% | |
| TTGTCGCCATACTATTACAA+TGG | - | chr6.1:4880536-4880555 | MS.gene074513:CDS | 35.0% | |
| ! | AGGTTTGAAAGATTCTGGAA+AGG | + | chr6.1:4880608-4880627 | None:intergenic | 35.0% |
| ! | ATAAAACCCCATTAGGGTTT+TGG | - | chr6.1:4880294-4880313 | MS.gene074513:CDS | 35.0% |
| ! | ATAGTATGAGCTTTGTGCAT+TGG | + | chr6.1:4880363-4880382 | None:intergenic | 35.0% |
| ! | TAAGTTGAAATCCATGTGGT+AGG | + | chr6.1:4880628-4880647 | None:intergenic | 35.0% |
| ! | TGTACCTCTCTTTTGTCTTT+AGG | + | chr6.1:4879864-4879883 | None:intergenic | 35.0% |
| !! | CCATTAAGTTGAAATCCATG+TGG | + | chr6.1:4880632-4880651 | None:intergenic | 35.0% |
| AACATTAGCATCAACTCGGT+TGG | + | chr6.1:4880459-4880478 | None:intergenic | 40.0% | |
| AAGTTGCCAAAACCCTAATG+GGG | + | chr6.1:4880303-4880322 | None:intergenic | 40.0% | |
| AGTTCAACTACATGGATGGA+AGG | - | chr6.1:4880684-4880703 | MS.gene074513:CDS | 40.0% | |
| ATGGCCTAAAGACAAAAGAG+AGG | - | chr6.1:4879857-4879876 | MS.gene074513:CDS | 40.0% | |
| CAACGGATGCATATGCTAAA+AGG | - | chr6.1:4880116-4880135 | MS.gene074513:CDS | 40.0% | |
| GAAGTTGCCAAAACCCTAAT+GGG | + | chr6.1:4880304-4880323 | None:intergenic | 40.0% | |
| GCAAATGACCTCAATTGTTC+CGG | + | chr6.1:4880062-4880081 | None:intergenic | 40.0% | |
| GCTCCATAGATATTCTTCCA+CGG | + | chr6.1:4879680-4879699 | None:intergenic | 40.0% | |
| GTGGTAGGTTTGAAAGATTC+TGG | + | chr6.1:4880613-4880632 | None:intergenic | 40.0% | |
| TATCTTCTCTTTGCGCACTT+TGG | + | chr6.1:4879994-4880013 | None:intergenic | 40.0% | |
| TGAAGTTGCCAAAACCCTAA+TGG | + | chr6.1:4880305-4880324 | None:intergenic | 40.0% | |
| TGCACAAAGCTCATACTATC+CGG | - | chr6.1:4880364-4880383 | MS.gene074513:CDS | 40.0% | |
| TTGATGTTAATTGGGCCAAC+TGG | - | chr6.1:4880432-4880451 | MS.gene074513:CDS | 40.0% | |
| TTGTCTTTAGGCCATGTTTC+CGG | + | chr6.1:4879852-4879871 | None:intergenic | 40.0% | |
| GCTTGCAAGGGTAAGATTGT+GGG | + | chr6.1:4880409-4880428 | None:intergenic | 45.0% | |
| GGAAGAGAATGCATAGCAAC+AGG | + | chr6.1:4880587-4880606 | None:intergenic | 45.0% | |
| TACCTACCTTCTTGAGACTC+CGG | + | chr6.1:4880386-4880405 | None:intergenic | 45.0% | |
| TACTATCCGGAGTCTCAAGA+AGG | - | chr6.1:4880377-4880396 | MS.gene074513:CDS | 45.0% | |
| TGCTTGCAAGGGTAAGATTG+TGG | + | chr6.1:4880410-4880429 | None:intergenic | 45.0% | |
| ! | GAGGTCATTTGCTAGCATGT+TGG | - | chr6.1:4880070-4880089 | MS.gene074513:CDS | 45.0% |
| !! | AGGGTTTTGGCAACTTCAAC+TGG | - | chr6.1:4880307-4880326 | MS.gene074513:CDS | 45.0% |
| !! | GAAGTAGCTACTTGGCTTTG+AGG | + | chr6.1:4880213-4880232 | None:intergenic | 45.0% |
| !! | GATTTGCTGAACCGGAAACA+TGG | - | chr6.1:4879838-4879857 | MS.gene074513:CDS | 45.0% |
| !! | GGGTTTTGGCAACTTCAACT+GGG | - | chr6.1:4880308-4880327 | MS.gene074513:CDS | 45.0% |
| !! | TTCTCCTAACTTTGAAGCCC+CGG | - | chr6.1:4879815-4879834 | MS.gene074513:CDS | 45.0% |
| AATCCCGGGGCTTCAAAGTT+AGG | + | chr6.1:4879822-4879841 | None:intergenic | 50.0% | |
| ATCCGGAGTCTCAAGAAGGT+AGG | - | chr6.1:4880381-4880400 | MS.gene074513:CDS | 50.0% | |
| CTCTTGGACCGGAACAATTG+AGG | - | chr6.1:4880051-4880070 | MS.gene074513:CDS | 50.0% | |
| GGGCCGTGGAAGAATATCTA+TGG | - | chr6.1:4879674-4879693 | MS.gene074513:CDS | 50.0% | |
| GTTTCCGGTTCAGCAAATCC+CGG | + | chr6.1:4879837-4879856 | None:intergenic | 50.0% | |
| TTTCCGGTTCAGCAAATCCC+GGG | + | chr6.1:4879836-4879855 | None:intergenic | 50.0% | |
| ! | ATCAACTCGGTTGGTCCAGT+TGG | + | chr6.1:4880450-4880469 | None:intergenic | 50.0% |
| !! | CTTGGCTTTGAGGCCAAGTT+AGG | + | chr6.1:4880203-4880222 | None:intergenic | 50.0% |
| !! | TCTCCTAACTTTGAAGCCCC+GGG | - | chr6.1:4879816-4879835 | MS.gene074513:CDS | 50.0% |
| TTCCGGTTCAGCAAATCCCG+GGG | + | chr6.1:4879835-4879854 | None:intergenic | 55.0% | |
| !! | AGCCCCGGGATTTGCTGAAC+CGG | - | chr6.1:4879830-4879849 | MS.gene074513:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr6.1 | gene | 4879672 | 4880712 | 4879672 | ID=MS.gene074513 |
| chr6.1 | mRNA | 4879672 | 4880712 | 4879672 | ID=MS.gene074513.t1;Parent=MS.gene074513 |
| chr6.1 | exon | 4879672 | 4880712 | 4879672 | ID=MS.gene074513.t1.exon1;Parent=MS.gene074513.t1 |
| chr6.1 | CDS | 4879672 | 4880712 | 4879672 | ID=cds.MS.gene074513.t1;Parent=MS.gene074513.t1 |
| Gene Sequence |
| Protein sequence |
