Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074615.t1 | PNX73683.1 | 94.1 | 101 | 6 | 0 | 23 | 123 | 78 | 178 | 7.60E-47 | 196.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074615.t1 | Q3EAL1 | 67.0 | 88 | 24 | 1 | 41 | 123 | 68 | 155 | 1.6e-24 | 113.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074615.t1 | A0A2K3L595 | 94.1 | 101 | 6 | 0 | 23 | 123 | 78 | 178 | 5.5e-47 | 196.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene074615.t1 | TF | OFP |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074615.t1 | MTR_0907s0020 | 93.277 | 119 | 8 | 0 | 1 | 119 | 64 | 182 | 3.76e-67 | 201 |
| MS.gene074615.t1 | MTR_7g105790 | 79.221 | 77 | 15 | 1 | 41 | 117 | 112 | 187 | 8.53e-40 | 132 |
| MS.gene074615.t1 | MTR_4g052040 | 63.768 | 69 | 25 | 0 | 41 | 109 | 101 | 169 | 2.27e-26 | 97.1 |
| MS.gene074615.t1 | MTR_5g079710 | 48.113 | 106 | 45 | 2 | 2 | 107 | 64 | 159 | 4.75e-25 | 95.5 |
| MS.gene074615.t1 | MTR_4g052060 | 61.538 | 65 | 25 | 0 | 41 | 105 | 95 | 159 | 1.95e-22 | 87.0 |
| MS.gene074615.t1 | MTR_8g103520 | 54.167 | 72 | 32 | 1 | 34 | 105 | 220 | 290 | 3.49e-20 | 83.6 |
| MS.gene074615.t1 | MTR_5g075210 | 53.226 | 62 | 29 | 0 | 39 | 100 | 280 | 341 | 3.70e-17 | 75.9 |
| MS.gene074615.t1 | MTR_4g028930 | 44.928 | 69 | 38 | 0 | 32 | 100 | 307 | 375 | 3.06e-16 | 73.6 |
| MS.gene074615.t1 | MTR_7g072875 | 43.478 | 69 | 39 | 0 | 32 | 100 | 212 | 280 | 5.25e-15 | 69.3 |
| MS.gene074615.t1 | MTR_7g072630 | 43.478 | 69 | 39 | 0 | 32 | 100 | 274 | 342 | 8.46e-15 | 69.3 |
| MS.gene074615.t1 | MTR_3g111780 | 56.061 | 66 | 29 | 0 | 39 | 104 | 198 | 263 | 1.00e-14 | 68.6 |
| MS.gene074615.t1 | MTR_8g087530 | 48.438 | 64 | 33 | 0 | 37 | 100 | 256 | 319 | 2.28e-14 | 68.2 |
| MS.gene074615.t1 | MTR_2g018030 | 38.961 | 77 | 43 | 1 | 42 | 114 | 280 | 356 | 3.50e-13 | 64.7 |
| MS.gene074615.t1 | MTR_4g132740 | 42.424 | 66 | 38 | 0 | 41 | 106 | 83 | 148 | 1.48e-12 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene074615.t1 | AT3G52525 | 80.000 | 65 | 13 | 0 | 36 | 100 | 63 | 127 | 1.29e-34 | 117 |
| MS.gene074615.t1 | AT2G36026 | 82.540 | 63 | 11 | 0 | 44 | 106 | 91 | 153 | 6.98e-34 | 116 |
| MS.gene074615.t1 | AT5G19650 | 52.239 | 67 | 32 | 0 | 39 | 105 | 154 | 220 | 2.85e-21 | 85.1 |
| MS.gene074615.t1 | AT2G18500 | 54.412 | 68 | 31 | 0 | 36 | 103 | 223 | 290 | 2.92e-21 | 86.7 |
| MS.gene074615.t1 | AT1G06920 | 39.796 | 98 | 49 | 2 | 16 | 113 | 228 | 315 | 2.37e-16 | 73.6 |
| MS.gene074615.t1 | AT2G30400 | 51.613 | 62 | 30 | 0 | 39 | 100 | 252 | 313 | 8.59e-16 | 72.0 |
| MS.gene074615.t1 | AT5G01840 | 53.448 | 58 | 27 | 0 | 43 | 100 | 202 | 259 | 8.18e-15 | 68.9 |
| MS.gene074615.t1 | AT4G18830 | 47.059 | 68 | 36 | 0 | 34 | 101 | 277 | 344 | 1.03e-14 | 69.3 |
| MS.gene074615.t1 | AT5G58360 | 47.458 | 59 | 31 | 0 | 42 | 100 | 227 | 285 | 2.07e-14 | 67.8 |
| MS.gene074615.t1 | AT5G22240 | 43.750 | 64 | 36 | 0 | 38 | 101 | 95 | 158 | 2.15e-13 | 63.9 |
Find 0 sgRNAs with CRISPR-Local
Find 20 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AATGTCGAAAATCAAGATAA+GGG | + | 10664:687-706 | MS.gene074615:intergenic | 25.0% |
| !! | AAATCATCTTTGAAAGGATT+TGG | - | 10664:616-635 | MS.gene074615:CDS | 25.0% |
| GAATGTCGAAAATCAAGATA+AGG | + | 10664:688-707 | MS.gene074615:intergenic | 30.0% | |
| TCAGAGAAATCATCTTTGAA+AGG | - | 10664:610-629 | MS.gene074615:CDS | 30.0% | |
| TTCAATGTTGCAGATGATAT+TGG | - | 10664:705-724 | MS.gene074615:CDS | 30.0% | |
| ! | TTAGAGCTTTTACTGAGATT+TGG | - | 10664:815-834 | MS.gene074615:CDS | 30.0% |
| ! | TTTTCTTCAACTGAATTCGA+AGG | - | 10664:774-793 | MS.gene074615:CDS | 30.0% |
| !!! | TTAAGTCTGGTTTTTCATCT+GGG | - | 10664:854-873 | MS.gene074615:CDS | 30.0% |
| TGAATTCGAAGGAACATCAT+GGG | - | 10664:785-804 | MS.gene074615:CDS | 35.0% | |
| TTGAAAGGATTTGGAAGAGT+TGG | - | 10664:625-644 | MS.gene074615:CDS | 35.0% | |
| TTTGGAAGAGTTGGAAATGA+AGG | - | 10664:634-653 | MS.gene074615:CDS | 35.0% | |
| ! | GCTTTTACTGAGATTTGGAA+TGG | - | 10664:820-839 | MS.gene074615:CDS | 35.0% |
| !!! | GTTAAGTCTGGTTTTTCATC+TGG | - | 10664:853-872 | MS.gene074615:CDS | 35.0% |
| CCGAATGATGTGTTTGTTCT+CGG | + | 10664:589-608 | MS.gene074614:intergenic | 40.0% | |
| CCGAGAACAAACACATCATT+CGG | - | 10664:586-605 | MS.gene074615:CDS | 40.0% | |
| CTGAATTCGAAGGAACATCA+TGG | - | 10664:784-803 | MS.gene074615:CDS | 40.0% | |
| ! | GGAGTTTTCTCTGTTAAGTC+TGG | - | 10664:841-860 | MS.gene074615:CDS | 40.0% |
| ! | TGGAAATGAAGGTGTAGCTG+TGG | - | 10664:645-664 | MS.gene074615:CDS | 45.0% |
| !! | GTTTGTTCTCGGTGTTGTGT+TGG | + | 10664:578-597 | MS.gene074615:intergenic | 45.0% |
| !! | CGGTGTTGTGTTGGTTGTGC+TGG | + | 10664:569-588 | MS.gene074615:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 10664 | gene | 538 | 909 | 538 | ID=MS.gene074615 |
| 10664 | mRNA | 538 | 909 | 538 | ID=MS.gene074615.t1;Parent=MS.gene074615 |
| 10664 | exon | 538 | 909 | 538 | ID=MS.gene074615.t1.exon1;Parent=MS.gene074615.t1 |
| 10664 | CDS | 538 | 909 | 538 | ID=cds.MS.gene074615.t1;Parent=MS.gene074615.t1 |
| Gene Sequence |
| Protein sequence |
