Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07465.t1 | XP_003614286.1 | 61.5 | 117 | 45 | 0 | 1 | 117 | 1 | 117 | 7.80E-29 | 136.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07465.t1 | Q9FJK3 | 37.6 | 117 | 72 | 1 | 1 | 116 | 1 | 117 | 4.7e-14 | 79.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07465.t1 | G7JY85 | 61.5 | 117 | 45 | 0 | 1 | 117 | 1 | 117 | 5.6e-29 | 136.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene07465.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07465.t1 | MTR_5g047580 | 61.538 | 117 | 45 | 0 | 1 | 117 | 1 | 117 | 2.51e-47 | 150 |
| MS.gene07465.t1 | MTR_4g028720 | 49.686 | 159 | 56 | 1 | 1 | 135 | 1 | 159 | 2.56e-40 | 132 |
| MS.gene07465.t1 | MTR_1g090783 | 49.612 | 129 | 63 | 1 | 3 | 129 | 2 | 130 | 5.25e-40 | 131 |
| MS.gene07465.t1 | MTR_7g011950 | 48.428 | 159 | 58 | 1 | 1 | 135 | 1 | 159 | 5.80e-40 | 131 |
| MS.gene07465.t1 | MTR_1g084950 | 57.025 | 121 | 52 | 0 | 1 | 121 | 1 | 121 | 1.30e-39 | 130 |
| MS.gene07465.t1 | MTR_1g077320 | 47.170 | 159 | 60 | 1 | 1 | 135 | 1 | 159 | 4.41e-39 | 129 |
| MS.gene07465.t1 | MTR_1g077390 | 42.038 | 157 | 67 | 1 | 1 | 133 | 1 | 157 | 2.29e-37 | 125 |
| MS.gene07465.t1 | MTR_1g077300 | 46.497 | 157 | 62 | 2 | 1 | 135 | 1 | 157 | 6.53e-37 | 124 |
| MS.gene07465.t1 | MTR_1g090710 | 47.107 | 121 | 64 | 0 | 1 | 121 | 1 | 121 | 1.49e-36 | 122 |
| MS.gene07465.t1 | MTR_1g090697 | 48.760 | 121 | 62 | 0 | 1 | 121 | 1 | 121 | 4.53e-35 | 119 |
| MS.gene07465.t1 | MTR_5g047560 | 49.587 | 121 | 45 | 2 | 1 | 121 | 1 | 105 | 1.96e-31 | 109 |
| MS.gene07465.t1 | MTR_3g065100 | 42.149 | 121 | 69 | 1 | 1 | 120 | 1 | 121 | 8.43e-21 | 84.7 |
| MS.gene07465.t1 | MTR_4g032620 | 33.051 | 118 | 78 | 1 | 1 | 117 | 1 | 118 | 5.74e-20 | 82.4 |
| MS.gene07465.t1 | MTR_3g466980 | 35.593 | 118 | 75 | 1 | 1 | 117 | 1 | 118 | 1.63e-19 | 80.9 |
| MS.gene07465.t1 | MTR_4g031910 | 35.036 | 137 | 86 | 2 | 1 | 134 | 1 | 137 | 2.64e-19 | 80.5 |
| MS.gene07465.t1 | MTR_8g036130 | 34.266 | 143 | 83 | 3 | 1 | 135 | 1 | 140 | 5.02e-19 | 80.1 |
| MS.gene07465.t1 | MTR_3g466830 | 35.878 | 131 | 81 | 2 | 3 | 132 | 2 | 130 | 1.07e-18 | 77.4 |
| MS.gene07465.t1 | MTR_4g032260 | 37.681 | 138 | 83 | 2 | 1 | 135 | 1 | 138 | 2.98e-17 | 73.6 |
| MS.gene07465.t1 | MTR_4g032290 | 37.956 | 137 | 82 | 2 | 1 | 134 | 1 | 137 | 5.54e-17 | 74.3 |
| MS.gene07465.t1 | MTR_2g035610 | 32.203 | 118 | 79 | 1 | 1 | 117 | 1 | 118 | 7.33e-17 | 71.6 |
| MS.gene07465.t1 | MTR_5g075380 | 32.407 | 108 | 72 | 1 | 1 | 107 | 1 | 108 | 6.93e-16 | 70.1 |
| MS.gene07465.t1 | MTR_3g466890 | 32.174 | 115 | 77 | 1 | 3 | 116 | 2 | 116 | 1.36e-15 | 69.3 |
| MS.gene07465.t1 | MTR_4g028800 | 39.189 | 74 | 43 | 1 | 1 | 74 | 1 | 72 | 2.58e-15 | 66.2 |
| MS.gene07465.t1 | MTR_3g031240 | 32.743 | 113 | 75 | 1 | 1 | 112 | 1 | 113 | 1.38e-14 | 67.0 |
| MS.gene07465.t1 | MTR_4g063790 | 28.319 | 113 | 80 | 1 | 1 | 112 | 1 | 113 | 2.04e-13 | 63.9 |
| MS.gene07465.t1 | MTR_3g467080 | 29.204 | 113 | 79 | 1 | 1 | 112 | 1 | 113 | 3.92e-13 | 62.8 |
| MS.gene07465.t1 | MTR_4g019670 | 30.075 | 133 | 85 | 4 | 1 | 126 | 1 | 132 | 2.53e-12 | 63.2 |
| MS.gene07465.t1 | MTR_2g016210 | 44.068 | 59 | 33 | 0 | 1 | 59 | 1 | 59 | 7.25e-12 | 60.8 |
| MS.gene07465.t1 | MTR_7g106510 | 27.778 | 108 | 77 | 1 | 3 | 109 | 6 | 113 | 7.87e-12 | 61.6 |
| MS.gene07465.t1 | MTR_3g031100 | 29.630 | 108 | 75 | 1 | 1 | 107 | 1 | 108 | 3.31e-11 | 58.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07465.t1 | AT5G26630 | 38.060 | 134 | 74 | 2 | 1 | 133 | 1 | 126 | 1.43e-21 | 86.3 |
| MS.gene07465.t1 | AT1G65330 | 35.484 | 124 | 79 | 1 | 3 | 125 | 2 | 125 | 8.53e-21 | 85.5 |
| MS.gene07465.t1 | AT1G65300 | 35.000 | 120 | 77 | 1 | 3 | 121 | 2 | 121 | 1.15e-20 | 85.1 |
| MS.gene07465.t1 | AT5G27960 | 38.596 | 114 | 69 | 1 | 5 | 117 | 3 | 116 | 3.84e-20 | 84.3 |
| MS.gene07465.t1 | AT5G26650 | 37.719 | 114 | 70 | 1 | 5 | 117 | 3 | 116 | 2.26e-19 | 82.8 |
| MS.gene07465.t1 | AT3G05860 | 32.353 | 136 | 87 | 2 | 1 | 135 | 1 | 132 | 9.11e-18 | 76.3 |
| MS.gene07465.t1 | AT3G05860 | 32.353 | 136 | 87 | 2 | 1 | 135 | 1 | 132 | 2.28e-17 | 75.9 |
| MS.gene07465.t1 | AT5G48670 | 37.288 | 118 | 73 | 1 | 1 | 117 | 1 | 118 | 2.53e-17 | 76.6 |
| MS.gene07465.t1 | AT3G05860 | 32.353 | 136 | 87 | 2 | 1 | 135 | 1 | 132 | 2.87e-17 | 75.9 |
| MS.gene07465.t1 | AT1G31630 | 32.759 | 116 | 76 | 2 | 3 | 117 | 2 | 116 | 2.67e-16 | 73.9 |
| MS.gene07465.t1 | AT1G31640 | 32.174 | 115 | 76 | 2 | 3 | 116 | 2 | 115 | 8.70e-15 | 70.1 |
| MS.gene07465.t1 | AT5G27810 | 31.818 | 88 | 59 | 1 | 31 | 117 | 3 | 90 | 1.81e-12 | 60.1 |
| MS.gene07465.t1 | AT2G28700 | 30.303 | 132 | 85 | 2 | 1 | 128 | 1 | 129 | 1.98e-12 | 63.2 |
| MS.gene07465.t1 | AT5G26580 | 33.898 | 118 | 63 | 3 | 1 | 117 | 1 | 104 | 4.73e-12 | 62.0 |
| MS.gene07465.t1 | AT1G22590 | 30.172 | 116 | 80 | 1 | 1 | 115 | 1 | 116 | 7.72e-12 | 59.7 |
Find 26 sgRNAs with CRISPR-Local
Find 27 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAGCTATCATAGACGAATTT+TGG | 0.195643 | 1.3:-55381981 | None:intergenic |
| AGCTTTCCTTGCTAAATATT+TGG | 0.216107 | 1.3:-55381803 | None:intergenic |
| TGCTAAATATTTGGAGATAA+AGG | 0.240702 | 1.3:-55381794 | None:intergenic |
| ATCTGGCCACACCTCTGTTT+TGG | 0.280469 | 1.3:-55381926 | None:intergenic |
| GTAGCTCTTAAGACCTTGTT+TGG | 0.358470 | 1.3:-55381959 | None:intergenic |
| TATGATTCTTGAACTTAAGA+AGG | 0.409403 | 1.3:+55382113 | MS.gene07465:CDS |
| TGTTTGGCACCCTCTAGATC+TGG | 0.457133 | 1.3:-55381943 | None:intergenic |
| AAGCCAAGGAAAACCTGAAA+AGG | 0.472288 | 1.3:+55382052 | MS.gene07465:CDS |
| TAAGTTCAAGAATCATAAGA+AGG | 0.499644 | 1.3:-55382107 | None:intergenic |
| TGCCTTCATGCGGAGAAAGA+AGG | 0.504023 | 1.3:+55381825 | MS.gene07465:CDS |
| GCAGAAGATTACTGAAGCCA+AGG | 0.517795 | 1.3:+55382038 | MS.gene07465:CDS |
| GCAAGGCCATGGCTAGGAGT+AGG | 0.521343 | 1.3:+55381761 | None:intergenic |
| AGAGAACTCACTATTCTTTG+TGG | 0.526508 | 1.3:+55381865 | MS.gene07465:CDS |
| CAAGGCCATGGCTAGGAGTA+GGG | 0.537523 | 1.3:+55381762 | None:intergenic |
| TTATCTCCAAATATTTAGCA+AGG | 0.539762 | 1.3:+55381797 | MS.gene07465:CDS |
| AGCCTTCTTTCTCCGCATGA+AGG | 0.564830 | 1.3:-55381827 | None:intergenic |
| GATCTAGAGGGTGCCAAACA+AGG | 0.565612 | 1.3:+55381946 | MS.gene07465:CDS |
| AGAGGTGTGGCCAGATCTAG+AGG | 0.570284 | 1.3:+55381933 | MS.gene07465:CDS |
| TGCATTGCAAGGCCATGGCT+AGG | 0.571000 | 1.3:+55381755 | None:intergenic |
| AGACAATCATGAGAAGAAGC+TGG | 0.571579 | 1.3:+55382083 | MS.gene07465:CDS |
| AAAGAAGGCTCTCTTTGAGA+AGG | 0.588827 | 1.3:+55381840 | MS.gene07465:CDS |
| CAAGGAAAGCTGCCTTCATG+CGG | 0.617357 | 1.3:+55381815 | MS.gene07465:CDS |
| TTTCACCCTACTCCTAGCCA+TGG | 0.617946 | 1.3:-55381767 | None:intergenic |
| TTAATTCCAAAACAGAGGTG+TGG | 0.619134 | 1.3:+55381920 | MS.gene07465:CDS |
| TAAGAAGGTAATTGATGACA+AGG | 0.625288 | 1.3:+55382128 | MS.gene07465:CDS |
| GAGGTGTGGCCAGATCTAGA+GGG | 0.694385 | 1.3:+55381934 | MS.gene07465:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAAGTTCAAGAATCATAAGA+AGG | - | chr1.3:55382110-55382129 | None:intergenic | 25.0% |
| ! | TATGATTCTTGAACTTAAGA+AGG | + | chr1.3:55382113-55382132 | MS.gene07465:CDS | 25.0% |
| ! | TGCTAAATATTTGGAGATAA+AGG | - | chr1.3:55381797-55381816 | None:intergenic | 25.0% |
| ! | TTATCTCCAAATATTTAGCA+AGG | + | chr1.3:55381797-55381816 | MS.gene07465:CDS | 25.0% |
| !!! | TGTTTTGGAATTAAAAGGAT+TGG | - | chr1.3:55381914-55381933 | None:intergenic | 25.0% |
| AGCTTTCCTTGCTAAATATT+TGG | - | chr1.3:55381806-55381825 | None:intergenic | 30.0% | |
| ! | TAAGAAGGTAATTGATGACA+AGG | + | chr1.3:55382128-55382147 | MS.gene07465:CDS | 30.0% |
| !! | TCCTTTTAATTCCAAAACAG+AGG | + | chr1.3:55381915-55381934 | MS.gene07465:CDS | 30.0% |
| !!! | ACCTCTGTTTTGGAATTAAA+AGG | - | chr1.3:55381919-55381938 | None:intergenic | 30.0% |
| AGAGAACTCACTATTCTTTG+TGG | + | chr1.3:55381865-55381884 | MS.gene07465:CDS | 35.0% | |
| TTAATTCCAAAACAGAGGTG+TGG | + | chr1.3:55381920-55381939 | MS.gene07465:CDS | 35.0% | |
| ! | CAGCTATCATAGACGAATTT+TGG | - | chr1.3:55381984-55382003 | None:intergenic | 35.0% |
| AAGCCAAGGAAAACCTGAAA+AGG | + | chr1.3:55382052-55382071 | MS.gene07465:CDS | 40.0% | |
| AGACAATCATGAGAAGAAGC+TGG | + | chr1.3:55382083-55382102 | MS.gene07465:CDS | 40.0% | |
| GTAGCTCTTAAGACCTTGTT+TGG | - | chr1.3:55381962-55381981 | None:intergenic | 40.0% | |
| ! | AAAGAAGGCTCTCTTTGAGA+AGG | + | chr1.3:55381840-55381859 | MS.gene07465:CDS | 40.0% |
| !!! | TGTCTTGTTTCAGCCTTTTC+AGG | - | chr1.3:55382068-55382087 | None:intergenic | 40.0% |
| GCAGAAGATTACTGAAGCCA+AGG | + | chr1.3:55382038-55382057 | MS.gene07465:CDS | 45.0% | |
| !!! | CAGCCTTTTCAGGTTTTCCT+TGG | - | chr1.3:55382058-55382077 | None:intergenic | 45.0% |
| AGCCTTCTTTCTCCGCATGA+AGG | - | chr1.3:55381830-55381849 | None:intergenic | 50.0% | |
| CAAGGAAAGCTGCCTTCATG+CGG | + | chr1.3:55381815-55381834 | MS.gene07465:CDS | 50.0% | |
| TGCCTTCATGCGGAGAAAGA+AGG | + | chr1.3:55381825-55381844 | MS.gene07465:CDS | 50.0% | |
| TGTTTGGCACCCTCTAGATC+TGG | - | chr1.3:55381946-55381965 | None:intergenic | 50.0% | |
| ! | ATCTGGCCACACCTCTGTTT+TGG | - | chr1.3:55381929-55381948 | None:intergenic | 50.0% |
| !! | GATCTAGAGGGTGCCAAACA+AGG | + | chr1.3:55381946-55381965 | MS.gene07465:CDS | 50.0% |
| GAGGTGTGGCCAGATCTAGA+GGG | + | chr1.3:55381934-55381953 | MS.gene07465:CDS | 55.0% | |
| ! | AGAGGTGTGGCCAGATCTAG+AGG | + | chr1.3:55381933-55381952 | MS.gene07465:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 55381769 | 55382176 | 55381769 | ID=MS.gene07465 |
| chr1.3 | mRNA | 55381769 | 55382176 | 55381769 | ID=MS.gene07465.t1;Parent=MS.gene07465 |
| chr1.3 | exon | 55381769 | 55382176 | 55381769 | ID=MS.gene07465.t1.exon1;Parent=MS.gene07465.t1 |
| chr1.3 | CDS | 55381769 | 55382176 | 55381769 | ID=cds.MS.gene07465.t1;Parent=MS.gene07465.t1 |
| Gene Sequence |
| Protein sequence |
