Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07466.t1 | XP_013468828.1 | 89.8 | 157 | 16 | 0 | 1 | 157 | 1 | 157 | 9.00E-69 | 269.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07466.t1 | Q9FJK3 | 44.0 | 159 | 86 | 3 | 1 | 156 | 1 | 159 | 4.0e-25 | 115.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07466.t1 | A0A072VXU8 | 89.8 | 157 | 16 | 0 | 1 | 157 | 1 | 157 | 6.5e-69 | 269.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene07466.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07466.t1 | MTR_1g077300 | 89.809 | 157 | 16 | 0 | 1 | 157 | 1 | 157 | 2.82e-92 | 265 |
| MS.gene07466.t1 | MTR_1g077320 | 76.730 | 159 | 35 | 1 | 1 | 157 | 1 | 159 | 1.57e-79 | 233 |
| MS.gene07466.t1 | MTR_4g028720 | 76.101 | 159 | 36 | 1 | 1 | 157 | 1 | 159 | 3.90e-77 | 226 |
| MS.gene07466.t1 | MTR_7g011950 | 76.101 | 159 | 36 | 1 | 1 | 157 | 1 | 159 | 5.42e-77 | 226 |
| MS.gene07466.t1 | MTR_1g077390 | 70.064 | 157 | 45 | 1 | 1 | 155 | 1 | 157 | 2.21e-73 | 217 |
| MS.gene07466.t1 | MTR_1g084950 | 76.923 | 156 | 34 | 1 | 1 | 154 | 1 | 156 | 6.77e-73 | 216 |
| MS.gene07466.t1 | MTR_5g047580 | 70.513 | 156 | 44 | 1 | 1 | 154 | 1 | 156 | 1.52e-72 | 215 |
| MS.gene07466.t1 | MTR_1g090783 | 57.143 | 154 | 64 | 1 | 2 | 153 | 1 | 154 | 1.07e-55 | 172 |
| MS.gene07466.t1 | MTR_5g047560 | 60.645 | 155 | 43 | 2 | 1 | 153 | 1 | 139 | 7.07e-55 | 169 |
| MS.gene07466.t1 | MTR_1g090697 | 55.769 | 156 | 67 | 1 | 1 | 154 | 1 | 156 | 2.14e-54 | 169 |
| MS.gene07466.t1 | MTR_1g090710 | 52.229 | 157 | 72 | 1 | 1 | 154 | 1 | 157 | 1.62e-51 | 161 |
| MS.gene07466.t1 | MTR_5g075380 | 37.821 | 156 | 94 | 2 | 1 | 153 | 1 | 156 | 1.36e-35 | 121 |
| MS.gene07466.t1 | MTR_4g063790 | 40.373 | 161 | 92 | 2 | 1 | 157 | 1 | 161 | 1.85e-35 | 121 |
| MS.gene07466.t1 | MTR_3g466980 | 41.830 | 153 | 86 | 2 | 1 | 150 | 1 | 153 | 4.79e-34 | 119 |
| MS.gene07466.t1 | MTR_4g032620 | 37.013 | 154 | 93 | 2 | 1 | 150 | 1 | 154 | 3.02e-33 | 117 |
| MS.gene07466.t1 | MTR_3g031240 | 37.736 | 159 | 95 | 2 | 1 | 155 | 1 | 159 | 1.66e-31 | 111 |
| MS.gene07466.t1 | MTR_3g065100 | 43.949 | 157 | 85 | 2 | 1 | 154 | 1 | 157 | 1.72e-31 | 113 |
| MS.gene07466.t1 | MTR_3g467080 | 37.126 | 167 | 89 | 3 | 1 | 157 | 1 | 161 | 1.92e-31 | 110 |
| MS.gene07466.t1 | MTR_3g466830 | 41.772 | 158 | 89 | 2 | 2 | 156 | 1 | 158 | 6.74e-31 | 109 |
| MS.gene07466.t1 | MTR_3g031100 | 37.662 | 154 | 92 | 2 | 1 | 150 | 1 | 154 | 9.58e-30 | 108 |
| MS.gene07466.t1 | MTR_4g031910 | 39.130 | 161 | 94 | 2 | 1 | 157 | 1 | 161 | 6.46e-29 | 106 |
| MS.gene07466.t1 | MTR_8g036130 | 38.125 | 160 | 93 | 2 | 1 | 154 | 1 | 160 | 1.03e-28 | 106 |
| MS.gene07466.t1 | MTR_4g032290 | 39.130 | 161 | 94 | 2 | 1 | 157 | 1 | 161 | 3.98e-27 | 101 |
| MS.gene07466.t1 | MTR_2g016210 | 37.662 | 154 | 92 | 2 | 1 | 150 | 1 | 154 | 3.46e-26 | 99.8 |
| MS.gene07466.t1 | MTR_4g032260 | 38.509 | 161 | 93 | 3 | 1 | 156 | 1 | 160 | 4.87e-26 | 97.1 |
| MS.gene07466.t1 | MTR_3g466890 | 37.107 | 159 | 97 | 2 | 2 | 157 | 1 | 159 | 5.16e-26 | 97.1 |
| MS.gene07466.t1 | MTR_2g035610 | 43.478 | 115 | 64 | 1 | 1 | 114 | 1 | 115 | 4.19e-25 | 93.6 |
| MS.gene07466.t1 | MTR_3g465410 | 36.090 | 133 | 81 | 2 | 29 | 157 | 1 | 133 | 7.01e-23 | 88.6 |
| MS.gene07466.t1 | MTR_4g028800 | 50.000 | 74 | 35 | 1 | 1 | 74 | 1 | 72 | 5.65e-21 | 81.6 |
| MS.gene07466.t1 | MTR_4g019670 | 33.600 | 125 | 78 | 2 | 3 | 126 | 6 | 126 | 5.86e-20 | 85.9 |
| MS.gene07466.t1 | MTR_7g106510 | 36.111 | 108 | 68 | 1 | 3 | 109 | 6 | 113 | 4.78e-19 | 83.2 |
| MS.gene07466.t1 | MTR_2g035580 | 35.948 | 153 | 95 | 2 | 1 | 150 | 1 | 153 | 8.79e-19 | 78.2 |
| MS.gene07466.t1 | MTR_3g466900 | 35.659 | 129 | 80 | 2 | 32 | 157 | 2 | 130 | 5.93e-18 | 75.5 |
| MS.gene07466.t1 | MTR_3g466930 | 34.884 | 129 | 81 | 2 | 32 | 157 | 2 | 130 | 1.52e-17 | 74.3 |
| MS.gene07466.t1 | MTR_3g093900 | 31.579 | 133 | 83 | 3 | 3 | 132 | 6 | 133 | 1.03e-15 | 73.6 |
| MS.gene07466.t1 | MTR_7g055800 | 36.522 | 115 | 44 | 3 | 1 | 114 | 1 | 87 | 3.06e-15 | 67.4 |
| MS.gene07466.t1 | MTR_7g055790 | 39.640 | 111 | 48 | 2 | 1 | 110 | 59 | 151 | 2.38e-14 | 66.6 |
| MS.gene07466.t1 | MTR_1g033930 | 40.000 | 100 | 55 | 2 | 62 | 157 | 20 | 118 | 6.50e-13 | 62.0 |
| MS.gene07466.t1 | MTR_1g033790 | 40.404 | 99 | 56 | 1 | 62 | 157 | 20 | 118 | 7.15e-13 | 62.0 |
| MS.gene07466.t1 | MTR_1g033370 | 38.298 | 94 | 55 | 1 | 64 | 154 | 28 | 121 | 9.95e-13 | 61.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07466.t1 | AT3G05860 | 46.491 | 114 | 60 | 1 | 1 | 113 | 1 | 114 | 1.49e-30 | 110 |
| MS.gene07466.t1 | AT3G05860 | 46.491 | 114 | 60 | 1 | 1 | 113 | 1 | 114 | 3.65e-30 | 110 |
| MS.gene07466.t1 | AT3G05860 | 46.491 | 114 | 60 | 1 | 1 | 113 | 1 | 114 | 5.11e-30 | 110 |
| MS.gene07466.t1 | AT5G26630 | 41.830 | 153 | 86 | 2 | 1 | 150 | 1 | 153 | 1.05e-29 | 108 |
| MS.gene07466.t1 | AT5G48670 | 44.444 | 153 | 82 | 3 | 1 | 150 | 1 | 153 | 1.28e-29 | 110 |
| MS.gene07466.t1 | AT1G65330 | 39.189 | 148 | 87 | 2 | 2 | 146 | 1 | 148 | 4.14e-28 | 105 |
| MS.gene07466.t1 | AT1G65300 | 36.184 | 152 | 94 | 2 | 2 | 150 | 1 | 152 | 1.05e-27 | 104 |
| MS.gene07466.t1 | AT5G26650 | 40.260 | 154 | 81 | 4 | 4 | 150 | 2 | 151 | 5.00e-27 | 104 |
| MS.gene07466.t1 | AT5G27960 | 45.045 | 111 | 60 | 1 | 4 | 113 | 2 | 112 | 1.78e-25 | 99.4 |
| MS.gene07466.t1 | AT1G22590 | 35.976 | 164 | 83 | 4 | 1 | 153 | 1 | 153 | 9.50e-24 | 91.3 |
| MS.gene07466.t1 | AT1G31630 | 31.847 | 157 | 99 | 4 | 2 | 153 | 1 | 154 | 1.52e-23 | 94.4 |
| MS.gene07466.t1 | AT1G31640 | 42.478 | 113 | 63 | 2 | 2 | 113 | 1 | 112 | 1.06e-21 | 90.5 |
| MS.gene07466.t1 | AT2G28700 | 38.596 | 114 | 69 | 1 | 1 | 113 | 1 | 114 | 6.17e-21 | 87.4 |
| MS.gene07466.t1 | AT5G27810 | 43.529 | 85 | 47 | 1 | 29 | 112 | 1 | 85 | 6.92e-19 | 77.4 |
| MS.gene07466.t1 | AT5G26580 | 38.938 | 113 | 56 | 1 | 1 | 113 | 1 | 100 | 2.98e-18 | 80.1 |
| MS.gene07466.t1 | AT2G40210 | 37.864 | 103 | 59 | 2 | 1 | 100 | 1 | 101 | 1.22e-11 | 61.6 |
Find 22 sgRNAs with CRISPR-Local
Find 24 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GCTGCAAGAGAAAAGTTTCT+TGG | 0.265401 | 1.3:-55375110 | None:intergenic |
| GAGCTAAAGATTCGTATAAA+TGG | 0.316885 | 1.3:+55375189 | MS.gene07466:CDS |
| CTCCTTTCTTCTTGAAGCAT+TGG | 0.353516 | 1.3:-55374893 | None:intergenic |
| AAATCTGAAGGAAGCAAAAC+AGG | 0.418438 | 1.3:+55375035 | MS.gene07466:CDS |
| TCTGAAACAGTCAAATCATC+TGG | 0.455315 | 1.3:-55375228 | None:intergenic |
| CTCCAATGCTTCAAGAAGAA+AGG | 0.468651 | 1.3:+55374891 | MS.gene07466:CDS |
| CAAGGCCATGGTTCGGAAGA+AGG | 0.471800 | 1.3:+55374852 | None:intergenic |
| TACATTGCAAGGCCATGGTT+CGG | 0.507534 | 1.3:+55374845 | None:intergenic |
| GACATACAGAAAGAGAAAGA+AGG | 0.512113 | 1.3:+55374915 | MS.gene07466:CDS |
| AGAGAACTCACTATTCTTTG+TGG | 0.526508 | 1.3:+55374955 | MS.gene07466:CDS |
| ACATACAGAAAGAGAAAGAA+GGG | 0.541098 | 1.3:+55374916 | MS.gene07466:CDS |
| TGCAGCAGATTACTAAAGCT+AGG | 0.546331 | 1.3:+55375127 | MS.gene07466:CDS |
| AAACAGTCAAATCATCTGGC+AGG | 0.548613 | 1.3:-55375224 | None:intergenic |
| AGGATCGGAAATTATAGCAC+AGG | 0.559456 | 1.3:-55374986 | None:intergenic |
| AGAGGTGTGGCCAAATCTGA+AGG | 0.570010 | 1.3:+55375023 | MS.gene07466:CDS |
| TCGGAAATTATAGCACAGGC+TGG | 0.570119 | 1.3:-55374982 | None:intergenic |
| AAAGAAGGGTATCATCAAGA+AGG | 0.577075 | 1.3:+55374930 | MS.gene07466:CDS |
| CTTCACCTTCTTCCGAACCA+TGG | 0.581108 | 1.3:-55374857 | None:intergenic |
| GCAGCAGATTACTAAAGCTA+GGG | 0.592338 | 1.3:+55375128 | MS.gene07466:CDS |
| AAGCAAAACAGGTGATTGAG+AGG | 0.611042 | 1.3:+55375046 | MS.gene07466:CDS |
| GAAACAAGACAATCGTGAGA+AGG | 0.628912 | 1.3:+55375167 | MS.gene07466:CDS |
| TTGATTCTAAAACAGAGGTG+TGG | 0.633348 | 1.3:+55375010 | MS.gene07466:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GATAATTGAGAAAAATATGA+AGG | + | chr1.3:55375269-55375288 | MS.gene07466:CDS | 20.0% |
| !!! | TGTTTTAGAATCAAAAGGAT+CGG | - | chr1.3:55375004-55375023 | None:intergenic | 25.0% |
| ACATACAGAAAGAGAAAGAA+GGG | + | chr1.3:55374916-55374935 | MS.gene07466:CDS | 30.0% | |
| GAGCTAAAGATTCGTATAAA+TGG | + | chr1.3:55375189-55375208 | MS.gene07466:CDS | 30.0% | |
| !!! | ACCTCTGTTTTAGAATCAAA+AGG | - | chr1.3:55375009-55375028 | None:intergenic | 30.0% |
| !!! | TCCTTTTGATTCTAAAACAG+AGG | + | chr1.3:55375005-55375024 | MS.gene07466:CDS | 30.0% |
| AAATCTGAAGGAAGCAAAAC+AGG | + | chr1.3:55375035-55375054 | MS.gene07466:CDS | 35.0% | |
| AGAGAACTCACTATTCTTTG+TGG | + | chr1.3:55374955-55374974 | MS.gene07466:CDS | 35.0% | |
| GACATACAGAAAGAGAAAGA+AGG | + | chr1.3:55374915-55374934 | MS.gene07466:CDS | 35.0% | |
| ! | TCTGAAACAGTCAAATCATC+TGG | - | chr1.3:55375231-55375250 | None:intergenic | 35.0% |
| ! | TGTTTTGCTTCCTTCAGATT+TGG | - | chr1.3:55375036-55375055 | None:intergenic | 35.0% |
| !! | AAAGAAGGGTATCATCAAGA+AGG | + | chr1.3:55374930-55374949 | MS.gene07466:CDS | 35.0% |
| !! | TTGATTCTAAAACAGAGGTG+TGG | + | chr1.3:55375010-55375029 | MS.gene07466:CDS | 35.0% |
| AAACAGTCAAATCATCTGGC+AGG | - | chr1.3:55375227-55375246 | None:intergenic | 40.0% | |
| AAGCAAAACAGGTGATTGAG+AGG | + | chr1.3:55375046-55375065 | MS.gene07466:CDS | 40.0% | |
| AGGATCGGAAATTATAGCAC+AGG | - | chr1.3:55374989-55375008 | None:intergenic | 40.0% | |
| CTCCAATGCTTCAAGAAGAA+AGG | + | chr1.3:55374891-55374910 | MS.gene07466:CDS | 40.0% | |
| CTCCTTTCTTCTTGAAGCAT+TGG | - | chr1.3:55374896-55374915 | None:intergenic | 40.0% | |
| GAAACAAGACAATCGTGAGA+AGG | + | chr1.3:55375167-55375186 | MS.gene07466:CDS | 40.0% | |
| GCAGCAGATTACTAAAGCTA+GGG | + | chr1.3:55375128-55375147 | MS.gene07466:CDS | 40.0% | |
| GCTGCAAGAGAAAAGTTTCT+TGG | - | chr1.3:55375113-55375132 | None:intergenic | 40.0% | |
| TGCAGCAGATTACTAAAGCT+AGG | + | chr1.3:55375127-55375146 | MS.gene07466:CDS | 40.0% | |
| TCGGAAATTATAGCACAGGC+TGG | - | chr1.3:55374985-55375004 | None:intergenic | 45.0% | |
| ! | AGAGGTGTGGCCAAATCTGA+AGG | + | chr1.3:55375023-55375042 | MS.gene07466:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 55374859 | 55375332 | 55374859 | ID=MS.gene07466 |
| chr1.3 | mRNA | 55374859 | 55375332 | 55374859 | ID=MS.gene07466.t1;Parent=MS.gene07466 |
| chr1.3 | exon | 55374859 | 55375332 | 55374859 | ID=MS.gene07466.t1.exon1;Parent=MS.gene07466.t1 |
| chr1.3 | CDS | 55374859 | 55375332 | 55374859 | ID=cds.MS.gene07466.t1;Parent=MS.gene07466.t1 |
| Gene Sequence |
| Protein sequence |
