AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07495

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07495.t1 MTR_1g077730 58.879 214 37 6 1 183 1 194 3.52e-71 214
MS.gene07495.t1 MTR_1g077700 69.110 191 46 5 1 183 1 186 2.22e-69 209
MS.gene07495.t1 MTR_1g090703 51.190 168 49 7 44 181 135 299 2.76e-37 132
MS.gene07495.t1 MTR_1g067640 38.710 124 60 3 63 182 38 149 1.15e-19 81.6
MS.gene07495.t1 MTR_1g067650 38.710 124 60 3 63 182 39 150 1.32e-19 81.6
MS.gene07495.t1 MTR_7g100100 40.650 123 57 4 63 182 34 143 1.41e-19 81.3
MS.gene07495.t1 MTR_2g084315 36.184 152 66 4 60 181 122 272 1.53e-18 81.3
MS.gene07495.t1 MTR_1g115345 46.914 81 37 1 62 142 44 118 6.21e-18 78.2
MS.gene07495.t1 MTR_1g062780 34.839 155 69 3 56 181 113 264 1.90e-17 78.2
MS.gene07495.t1 MTR_7g023560 49.398 83 31 2 62 144 39 110 1.10e-16 74.3
MS.gene07495.t1 MTR_2g096540 33.333 132 60 2 47 178 18 121 1.79e-16 72.4
MS.gene07495.t1 MTR_1g067660 38.710 124 59 5 63 182 35 145 6.31e-16 71.6
MS.gene07495.t1 MTR_4g027440 41.975 81 46 1 63 142 220 300 6.49e-14 68.9
MS.gene07495.t1 MTR_4g028610 34.416 154 66 6 62 181 226 378 1.37e-13 68.2
MS.gene07495.t1 MTR_1g018420 33.613 119 72 2 63 181 81 192 6.23e-12 62.4
MS.gene07495.t1 MTR_1g106730 40.000 80 39 1 63 142 83 153 8.20e-12 62.4
MS.gene07495.t1 MTR_0160s0030 33.871 124 60 4 58 181 235 336 1.77e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07495.t1 AT3G10470 42.775 173 48 8 58 181 177 347 1.75e-28 109
MS.gene07495.t1 AT2G28200 42.208 154 59 6 56 181 108 259 9.31e-27 103
MS.gene07495.t1 AT5G04390 41.081 185 60 8 41 181 136 315 9.42e-27 104
MS.gene07495.t1 AT2G37430 40.602 133 56 5 56 182 40 155 6.09e-20 82.8
MS.gene07495.t1 AT5G03510 36.620 142 70 3 61 182 116 257 1.33e-18 81.3
MS.gene07495.t1 AT3G53600 31.677 161 86 5 23 182 15 152 1.16e-17 76.6
MS.gene07495.t1 AT5G59820 30.899 178 87 6 8 180 1 147 2.59e-16 72.8
MS.gene07495.t1 AT3G46090 36.585 123 63 2 61 179 38 149 4.67e-16 72.4
MS.gene07495.t1 AT3G46080 43.902 82 37 1 61 142 35 107 6.66e-15 69.3
MS.gene07495.t1 AT2G28710 39.216 102 43 3 41 142 18 100 1.45e-14 68.2
MS.gene07495.t1 AT3G49930 42.353 85 37 2 63 142 94 171 8.16e-14 67.4
MS.gene07495.t1 AT3G60580 47.500 80 36 2 63 142 173 246 1.67e-13 67.4
MS.gene07495.t1 AT2G45120 35.294 119 64 2 63 181 194 299 2.43e-13 67.0
MS.gene07495.t1 AT3G46070 35.870 92 52 1 51 142 24 108 8.62e-13 63.5
MS.gene07495.t1 AT1G02030 38.372 86 45 1 59 144 156 233 9.20e-13 65.1
MS.gene07495.t1 AT5G04340 38.554 83 49 1 62 142 88 170 1.24e-12 64.3
MS.gene07495.t1 AT5G43170 43.750 80 31 3 63 142 75 140 2.41e-12 62.8
MS.gene07495.t1 AT5G67450 38.889 90 45 2 63 142 97 186 4.96e-12 62.8
MS.gene07495.t1 AT1G27730 43.210 81 43 2 63 142 80 158 8.95e-12 62.0
MS.gene07495.t1 AT4G35280 33.333 132 64 3 54 182 153 263 1.01e-11 62.4
MS.gene07495.t1 AT5G61470 39.785 93 44 3 50 142 216 296 2.55e-11 61.2
MS.gene07495.t1 AT3G19580 36.585 82 50 1 63 142 106 187 3.87e-11 60.5
MS.gene07495.t1 AT3G19580 36.585 82 50 1 63 142 106 187 3.87e-11 60.5
MS.gene07495.t1 AT5G56200 38.144 97 47 3 63 146 342 438 4.61e-11 61.2
MS.gene07495.t1 AT2G17180 31.429 140 69 4 50 183 135 253 7.41e-11 59.7

Find 49 sgRNAs with CRISPR-Local

Find 54 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAACAAATCCAACGTTTA+TGG 0.181089 1.2:-63093060 MS.gene07495:CDS
AACGAATGTCTCTTTGGATT+TGG 0.231339 1.2:-63092893 MS.gene07495:CDS
ATTCACTTTGGTGACATTAA+TGG 0.242417 1.2:+63093350 None:intergenic
CTCATTAACACCAATGGCTT+TGG 0.300691 1.2:-63092953 MS.gene07495:CDS
GGTTCTTCATCTTCTTTGTC+TGG 0.341589 1.2:+63092928 None:intergenic
AGTGGTGGTGGAGGTGGTGT+TGG 0.362843 1.2:+63093101 None:intergenic
ACAACAAATCCAACGTTTAT+GGG 0.395741 1.2:-63093059 MS.gene07495:CDS
GAGGTCGCCTCCACCGGTGC+AGG 0.405277 1.2:+63092973 None:intergenic
TGTGGTTCTAAATTCACATC+TGG 0.409768 1.2:-63093027 MS.gene07495:CDS
GTTGGTAGTGGTGGTGGAGG+TGG 0.418761 1.2:+63093095 None:intergenic
TATCTTTGATCTTCTTAAGC+TGG 0.420628 1.2:+63092856 None:intergenic
GTTTATGGGTGCTCTATTTG+TGG 0.429587 1.2:-63093045 MS.gene07495:CDS
GAATAAGCAATGATGGAGAA+AGG 0.431980 1.2:+63093288 None:intergenic
TTCACTTCATTTCAAGCACT+TGG 0.432350 1.2:-63093186 MS.gene07495:CDS
GTTGTTGTTGGTAGTGGTGG+TGG 0.447547 1.2:+63093089 None:intergenic
ACATCTGGCCAGGCCCTTGG+TGG 0.451587 1.2:-63093012 MS.gene07495:CDS
GTTAATGAGGTCGCCTCCAC+CGG 0.455728 1.2:+63092967 None:intergenic
TTCACATCTGGCCAGGCCCT+TGG 0.458195 1.2:-63093015 MS.gene07495:CDS
TTGTCTGGTTCCAAAGCCAT+TGG 0.473795 1.2:+63092943 None:intergenic
CAAAGTGAATATAACTCAAA+AGG 0.474354 1.2:-63093337 MS.gene07495:CDS
AGTAGAAGAATAAGCAATGA+TGG 0.477070 1.2:+63093281 None:intergenic
CCTAGAAAAGCTCATAGAAA+AGG 0.480382 1.2:-63093138 MS.gene07495:CDS
TCATATGGCCACCAAGGGCC+TGG 0.484803 1.2:+63093004 None:intergenic
GTTGTTGGTAGTGGTGGTGG+AGG 0.487921 1.2:+63093092 None:intergenic
GTGCAGGGTGGAAGTTCATA+TGG 0.495014 1.2:+63092989 None:intergenic
CTTCCACCCTGCACCGGTGG+AGG 0.496858 1.2:-63092980 MS.gene07495:CDS
AATAAGCAATGATGGAGAAA+GGG 0.498901 1.2:+63093289 None:intergenic
TTCTAAATTCACATCTGGCC+AGG 0.516675 1.2:-63093022 MS.gene07495:CDS
GTTGTTGTTGTTGGTAGTGG+TGG 0.519273 1.2:+63093086 None:intergenic
AGAAAGGGATAGGAGATTGT+GGG 0.519333 1.2:+63093304 None:intergenic
ACTTCATTTCAAGCACTTGG+AGG 0.520190 1.2:-63093183 MS.gene07495:CDS
GCTTATTCTTCTACTAGAGA+AGG 0.521996 1.2:-63093273 MS.gene07495:CDS
GCAATGATGGAGAAAGGGAT+AGG 0.524368 1.2:+63093294 None:intergenic
CAAAGCCATTGGTGTTAATG+AGG 0.530269 1.2:+63092954 None:intergenic
GAGAAAGGGATAGGAGATTG+TGG 0.535781 1.2:+63093303 None:intergenic
AAAGGGATAGGAGATTGTGG+GGG 0.541180 1.2:+63093306 None:intergenic
AGGTCGCCTCCACCGGTGCA+GGG 0.559156 1.2:+63092974 None:intergenic
GAAAGGGATAGGAGATTGTG+GGG 0.561722 1.2:+63093305 None:intergenic
GATCTTCTTAAGCTGGAGGA+AGG 0.566486 1.2:+63092863 None:intergenic
GAACTTCCACCCTGCACCGG+TGG 0.571423 1.2:-63092983 MS.gene07495:CDS
TATGAACTTCCACCCTGCAC+CGG 0.571843 1.2:-63092986 MS.gene07495:CDS
CTTTGATCTTCTTAAGCTGG+AGG 0.575809 1.2:+63092859 None:intergenic
GGCGACCTCATTAACACCAA+TGG 0.589385 1.2:-63092959 MS.gene07495:CDS
AAGGGATAGGAGATTGTGGG+GGG 0.595859 1.2:+63093307 None:intergenic
GGAAGTTCATATGGCCACCA+AGG 0.622379 1.2:+63092998 None:intergenic
TATTCTTCTACTAGAGAAGG+AGG 0.628197 1.2:-63093270 MS.gene07495:CDS
GAAGTTCATATGGCCACCAA+GGG 0.630438 1.2:+63092999 None:intergenic
AATAGAGCACCCATAAACGT+TGG 0.630895 1.2:+63093050 None:intergenic
TCGCCTCCACCGGTGCAGGG+TGG 0.663528 1.2:+63092977 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
! CAAAGTGAATATAACTCAAA+AGG - chr1.2:63092932-63092951 MS.gene07495:CDS 25.0%
!!! CTTTTGAGTTATATTCACTT+TGG + chr1.2:63092934-63092953 None:intergenic 25.0%
AACAACAAATCCAACGTTTA+TGG - chr1.2:63093209-63093228 MS.gene07495:CDS 30.0%
ACAACAAATCCAACGTTTAT+GGG - chr1.2:63093210-63093229 MS.gene07495:CDS 30.0%
AGAAAAAACGAATGTCTCTT+TGG - chr1.2:63093370-63093389 MS.gene07495:CDS 30.0%
AGTAGAAGAATAAGCAATGA+TGG + chr1.2:63092991-63093010 None:intergenic 30.0%
! AATAAGCAATGATGGAGAAA+GGG + chr1.2:63092983-63093002 None:intergenic 30.0%
! ATTCACTTTGGTGACATTAA+TGG + chr1.2:63092922-63092941 None:intergenic 30.0%
AACGAATGTCTCTTTGGATT+TGG - chr1.2:63093376-63093395 MS.gene07495:CDS 35.0%
CCTAGAAAAGCTCATAGAAA+AGG - chr1.2:63093131-63093150 MS.gene07495:CDS 35.0%
GCTTATTCTTCTACTAGAGA+AGG - chr1.2:63092996-63093015 MS.gene07495:CDS 35.0%
TATTCTTCTACTAGAGAAGG+AGG - chr1.2:63092999-63093018 MS.gene07495:CDS 35.0%
TTCACTTCATTTCAAGCACT+TGG - chr1.2:63093083-63093102 MS.gene07495:CDS 35.0%
! CCTTTTCTATGAGCTTTTCT+AGG + chr1.2:63093134-63093153 None:intergenic 35.0%
! GAATAAGCAATGATGGAGAA+AGG + chr1.2:63092984-63093003 None:intergenic 35.0%
! GCTTCTTGTGAACTTTTCTA+TGG + chr1.2:63093112-63093131 None:intergenic 35.0%
! GTTGTTGTTGTTGTTGTTGT+TGG + chr1.2:63093195-63093214 None:intergenic 35.0%
!! TGTGGTTCTAAATTCACATC+TGG - chr1.2:63093242-63093261 MS.gene07495:CDS 35.0%
ACTTCATTTCAAGCACTTGG+AGG - chr1.2:63093086-63093105 MS.gene07495:CDS 40.0%
AGAAAGGGATAGGAGATTGT+GGG + chr1.2:63092968-63092987 None:intergenic 40.0%
CTCATTAACACCAATGGCTT+TGG - chr1.2:63093316-63093335 MS.gene07495:CDS 40.0%
GGTTCTTCATCTTCTTTGTC+TGG + chr1.2:63093344-63093363 None:intergenic 40.0%
GTTTATGGGTGCTCTATTTG+TGG - chr1.2:63093224-63093243 MS.gene07495:CDS 40.0%
TTCTAAATTCACATCTGGCC+AGG - chr1.2:63093247-63093266 MS.gene07495:CDS 40.0%
! AATAGAGCACCCATAAACGT+TGG + chr1.2:63093222-63093241 None:intergenic 40.0%
! GTTGTTGTTGTTGTTGGTAG+TGG + chr1.2:63093189-63093208 None:intergenic 40.0%
!! CAAAGCCATTGGTGTTAATG+AGG + chr1.2:63093318-63093337 None:intergenic 40.0%
!!! ACATTCGTTTTTTCTGCAGG+TGG + chr1.2:63093365-63093384 None:intergenic 40.0%
!!! GAGACATTCGTTTTTTCTGC+AGG + chr1.2:63093368-63093387 None:intergenic 40.0%
AAAGGGATAGGAGATTGTGG+GGG + chr1.2:63092966-63092985 None:intergenic 45.0%
GAAAGGGATAGGAGATTGTG+GGG + chr1.2:63092967-63092986 None:intergenic 45.0%
GAAGTTCATATGGCCACCAA+GGG + chr1.2:63093273-63093292 None:intergenic 45.0%
GAGAAAGGGATAGGAGATTG+TGG + chr1.2:63092969-63092988 None:intergenic 45.0%
! GCAATGATGGAGAAAGGGAT+AGG + chr1.2:63092978-63092997 None:intergenic 45.0%
! TTGTCTGGTTCCAAAGCCAT+TGG + chr1.2:63093329-63093348 None:intergenic 45.0%
!! GTTGTTGTTGTTGGTAGTGG+TGG + chr1.2:63093186-63093205 None:intergenic 45.0%
AAGGGATAGGAGATTGTGGG+GGG + chr1.2:63092965-63092984 None:intergenic 50.0%
GGAAGTTCATATGGCCACCA+AGG + chr1.2:63093274-63093293 None:intergenic 50.0%
GGCGACCTCATTAACACCAA+TGG - chr1.2:63093310-63093329 MS.gene07495:CDS 50.0%
GTGCAGGGTGGAAGTTCATA+TGG + chr1.2:63093283-63093302 None:intergenic 50.0%
TATGAACTTCCACCCTGCAC+CGG - chr1.2:63093283-63093302 MS.gene07495:CDS 50.0%
!! GTTGTTGTTGGTAGTGGTGG+TGG + chr1.2:63093183-63093202 None:intergenic 50.0%
GTTAATGAGGTCGCCTCCAC+CGG + chr1.2:63093305-63093324 None:intergenic 55.0%
!! GTTGTTGGTAGTGGTGGTGG+AGG + chr1.2:63093180-63093199 None:intergenic 55.0%
TCATATGGCCACCAAGGGCC+TGG + chr1.2:63093268-63093287 None:intergenic 60.0%
TTCACATCTGGCCAGGCCCT+TGG - chr1.2:63093254-63093273 MS.gene07495:CDS 60.0%
!! AGTGGTGGTGGAGGTGGTGT+TGG + chr1.2:63093171-63093190 None:intergenic 60.0%
!! GTTGGTAGTGGTGGTGGAGG+TGG + chr1.2:63093177-63093196 None:intergenic 60.0%
ACATCTGGCCAGGCCCTTGG+TGG - chr1.2:63093257-63093276 MS.gene07495:CDS 65.0%
GAACTTCCACCCTGCACCGG+TGG - chr1.2:63093286-63093305 MS.gene07495:CDS 65.0%
AGGTCGCCTCCACCGGTGCA+GGG + chr1.2:63093298-63093317 None:intergenic 70.0%
! CTTCCACCCTGCACCGGTGG+AGG - chr1.2:63093289-63093308 MS.gene07495:CDS 70.0%
GAGGTCGCCTCCACCGGTGC+AGG + chr1.2:63093299-63093318 None:intergenic 75.0%
TCGCCTCCACCGGTGCAGGG+TGG + chr1.2:63093295-63093314 None:intergenic 75.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 63092870 63093421 63092870 ID=MS.gene07495
chr1.2 mRNA 63092870 63093421 63092870 ID=MS.gene07495.t1;Parent=MS.gene07495
chr1.2 exon 63092870 63093421 63092870 ID=MS.gene07495.t1.exon1;Parent=MS.gene07495.t1
chr1.2 CDS 63092870 63093421 63092870 ID=cds.MS.gene07495.t1;Parent=MS.gene07495.t1
Gene Sequence

>MS.gene07495

ATGGAAGCATCTCACGAACAACAACTCTCACTAAACTCTACAGAACACACCATTAATGTCACCAAAGTGAATATAACTCAAAAGGTTATAAACCCCCCACAATCTCCTATCCCTTTCTCCATCATTGCTTATTCTTCTACTAGAGAAGGAGGAACAAGAACAAGAGAAAGTGCACGTTACAATGTGTATACATGCAAAACATGCAACAAAACATTCACTTCATTTCAAGCACTTGGAGGCCATAGAAAAGTTCACAAGAAGCCTAGAAAAGCTCATAGAAAAGGTCACAACAACAACACCAACACCACCTCCACCACCACTACCAACAACAACAACAACAACAACAAATCCAACGTTTATGGGTGCTCTATTTGTGGTTCTAAATTCACATCTGGCCAGGCCCTTGGTGGCCATATGAACTTCCACCCTGCACCGGTGGAGGCGACCTCATTAACACCAATGGCTTTGGAACCAGACAAAGAAGATGAAGAACCACCTGCAGAAAAAACGAATGTCTCTTTGGATTTGGATCTCAACCTTCCTCCAGCTTAA
Protein sequence

>MS.gene07495.t1

MEASHEQQLSLNSTEHTINVTKVNITQKVINPPQSPIPFSIIAYSSTREGGTRTRESARYNVYTCKTCNKTFTSFQALGGHRKVHKKPRKAHRKGHNNNTNTTSTTTTNNNNNNNKSNVYGCSICGSKFTSGQALGGHMNFHPAPVEATSLTPMALEPDKEDEEPPAEKTNVSLDLDLNLPPA