Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07497.t1 | XP_013468829.1 | 95.6 | 159 | 7 | 0 | 1 | 159 | 1 | 159 | 1.00E-75 | 292.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07497.t1 | Q9FJK3 | 44.9 | 156 | 85 | 1 | 1 | 155 | 1 | 156 | 1.1e-27 | 124.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07497.t1 | A0A072VMQ1 | 95.6 | 159 | 7 | 0 | 1 | 159 | 1 | 159 | 7.2e-76 | 292.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene07497.t1 | TF | MADS-M-type |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07497.t1 | MTR_1g077320 | 95.597 | 159 | 7 | 0 | 1 | 159 | 1 | 159 | 1.42e-102 | 291 |
| MS.gene07497.t1 | MTR_7g011950 | 91.824 | 159 | 13 | 0 | 1 | 159 | 1 | 159 | 4.18e-98 | 280 |
| MS.gene07497.t1 | MTR_4g028720 | 88.679 | 159 | 18 | 0 | 1 | 159 | 1 | 159 | 7.02e-96 | 274 |
| MS.gene07497.t1 | MTR_1g077390 | 71.338 | 157 | 45 | 0 | 1 | 157 | 1 | 157 | 2.47e-79 | 232 |
| MS.gene07497.t1 | MTR_5g047580 | 69.872 | 156 | 47 | 0 | 1 | 156 | 1 | 156 | 2.90e-76 | 224 |
| MS.gene07497.t1 | MTR_1g077300 | 76.101 | 159 | 36 | 1 | 1 | 159 | 1 | 157 | 3.00e-76 | 224 |
| MS.gene07497.t1 | MTR_1g084950 | 75.000 | 156 | 39 | 0 | 1 | 156 | 1 | 156 | 4.07e-76 | 224 |
| MS.gene07497.t1 | MTR_1g090697 | 56.774 | 155 | 67 | 0 | 1 | 155 | 1 | 155 | 4.18e-60 | 183 |
| MS.gene07497.t1 | MTR_1g090710 | 55.769 | 156 | 68 | 1 | 1 | 155 | 1 | 156 | 2.39e-58 | 179 |
| MS.gene07497.t1 | MTR_5g047560 | 58.974 | 156 | 48 | 1 | 1 | 156 | 1 | 140 | 3.76e-58 | 178 |
| MS.gene07497.t1 | MTR_1g090783 | 54.902 | 153 | 69 | 0 | 3 | 155 | 2 | 154 | 3.91e-56 | 173 |
| MS.gene07497.t1 | MTR_3g065100 | 49.682 | 157 | 78 | 1 | 1 | 156 | 1 | 157 | 2.14e-39 | 133 |
| MS.gene07497.t1 | MTR_3g466980 | 45.098 | 153 | 83 | 1 | 1 | 152 | 1 | 153 | 8.70e-39 | 131 |
| MS.gene07497.t1 | MTR_5g075380 | 39.103 | 156 | 94 | 1 | 1 | 155 | 1 | 156 | 1.69e-37 | 126 |
| MS.gene07497.t1 | MTR_4g032620 | 38.065 | 155 | 94 | 2 | 1 | 153 | 1 | 155 | 7.82e-37 | 127 |
| MS.gene07497.t1 | MTR_4g063790 | 40.252 | 159 | 93 | 2 | 1 | 157 | 1 | 159 | 7.29e-35 | 120 |
| MS.gene07497.t1 | MTR_3g466830 | 42.038 | 157 | 90 | 1 | 3 | 158 | 2 | 158 | 2.13e-33 | 115 |
| MS.gene07497.t1 | MTR_4g031910 | 43.333 | 150 | 83 | 2 | 1 | 148 | 1 | 150 | 5.00e-32 | 114 |
| MS.gene07497.t1 | MTR_8g036130 | 38.994 | 159 | 93 | 2 | 1 | 155 | 1 | 159 | 9.78e-32 | 114 |
| MS.gene07497.t1 | MTR_3g466890 | 40.260 | 154 | 91 | 1 | 3 | 155 | 2 | 155 | 1.51e-30 | 108 |
| MS.gene07497.t1 | MTR_2g016210 | 41.875 | 160 | 89 | 3 | 1 | 157 | 1 | 159 | 2.93e-30 | 110 |
| MS.gene07497.t1 | MTR_4g032260 | 42.500 | 160 | 90 | 2 | 1 | 158 | 1 | 160 | 7.68e-30 | 107 |
| MS.gene07497.t1 | MTR_3g031240 | 34.969 | 163 | 96 | 3 | 1 | 157 | 1 | 159 | 6.40e-29 | 105 |
| MS.gene07497.t1 | MTR_4g032290 | 44.667 | 150 | 81 | 2 | 1 | 148 | 1 | 150 | 1.17e-28 | 105 |
| MS.gene07497.t1 | MTR_3g467080 | 35.185 | 162 | 101 | 3 | 1 | 159 | 1 | 161 | 4.36e-28 | 102 |
| MS.gene07497.t1 | MTR_3g031100 | 36.076 | 158 | 97 | 3 | 1 | 155 | 1 | 157 | 5.56e-28 | 104 |
| MS.gene07497.t1 | MTR_2g035610 | 42.373 | 118 | 67 | 1 | 1 | 117 | 1 | 118 | 1.96e-25 | 94.4 |
| MS.gene07497.t1 | MTR_3g465410 | 35.878 | 131 | 82 | 2 | 29 | 157 | 1 | 131 | 8.14e-22 | 85.9 |
| MS.gene07497.t1 | MTR_2g035580 | 32.903 | 155 | 103 | 1 | 1 | 154 | 1 | 155 | 1.03e-21 | 85.9 |
| MS.gene07497.t1 | MTR_3g466900 | 37.600 | 125 | 77 | 1 | 32 | 155 | 2 | 126 | 4.80e-20 | 80.9 |
| MS.gene07497.t1 | MTR_4g028800 | 46.575 | 73 | 37 | 1 | 1 | 73 | 1 | 71 | 6.85e-19 | 76.3 |
| MS.gene07497.t1 | MTR_3g466930 | 36.000 | 125 | 79 | 1 | 32 | 155 | 2 | 126 | 9.51e-19 | 77.4 |
| MS.gene07497.t1 | MTR_4g019670 | 35.833 | 120 | 73 | 2 | 1 | 116 | 1 | 120 | 1.67e-17 | 79.0 |
| MS.gene07497.t1 | MTR_7g106510 | 35.185 | 108 | 69 | 1 | 3 | 109 | 6 | 113 | 1.08e-16 | 76.3 |
| MS.gene07497.t1 | MTR_7g055800 | 33.333 | 117 | 51 | 2 | 1 | 117 | 1 | 90 | 2.31e-15 | 67.8 |
| MS.gene07497.t1 | MTR_1g033930 | 39.796 | 98 | 58 | 1 | 62 | 158 | 20 | 117 | 2.88e-14 | 65.5 |
| MS.gene07497.t1 | MTR_1g033370 | 42.553 | 94 | 53 | 1 | 64 | 156 | 28 | 121 | 3.01e-14 | 65.5 |
| MS.gene07497.t1 | MTR_1g033790 | 40.816 | 98 | 57 | 1 | 62 | 158 | 20 | 117 | 1.17e-13 | 63.9 |
| MS.gene07497.t1 | MTR_7g055940 | 35.238 | 105 | 41 | 3 | 1 | 105 | 1 | 78 | 6.71e-13 | 60.8 |
| MS.gene07497.t1 | MTR_6g005450 | 31.250 | 112 | 71 | 3 | 1 | 106 | 1 | 112 | 2.24e-12 | 63.9 |
| MS.gene07497.t1 | MTR_7g055790 | 34.545 | 110 | 53 | 2 | 1 | 109 | 59 | 150 | 4.02e-12 | 60.8 |
| MS.gene07497.t1 | MTR_3g093900 | 26.974 | 152 | 106 | 2 | 3 | 151 | 6 | 155 | 1.10e-11 | 62.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07497.t1 | AT5G48670 | 45.098 | 153 | 83 | 1 | 1 | 152 | 1 | 153 | 1.09e-33 | 120 |
| MS.gene07497.t1 | AT3G05860 | 40.940 | 149 | 87 | 1 | 1 | 148 | 1 | 149 | 2.62e-33 | 117 |
| MS.gene07497.t1 | AT3G05860 | 40.940 | 149 | 87 | 1 | 1 | 148 | 1 | 149 | 1.46e-32 | 116 |
| MS.gene07497.t1 | AT3G05860 | 40.940 | 149 | 87 | 1 | 1 | 148 | 1 | 149 | 2.62e-32 | 115 |
| MS.gene07497.t1 | AT5G26630 | 38.750 | 160 | 97 | 1 | 1 | 159 | 1 | 160 | 2.43e-29 | 107 |
| MS.gene07497.t1 | AT1G65300 | 36.620 | 142 | 89 | 1 | 3 | 143 | 2 | 143 | 4.18e-27 | 102 |
| MS.gene07497.t1 | AT1G65330 | 36.054 | 147 | 93 | 1 | 3 | 148 | 2 | 148 | 9.41e-27 | 102 |
| MS.gene07497.t1 | AT5G26650 | 34.641 | 153 | 99 | 1 | 4 | 155 | 2 | 154 | 2.91e-24 | 97.1 |
| MS.gene07497.t1 | AT5G27960 | 39.437 | 142 | 78 | 4 | 4 | 142 | 2 | 138 | 2.87e-23 | 93.6 |
| MS.gene07497.t1 | AT2G28700 | 34.868 | 152 | 92 | 2 | 1 | 148 | 1 | 149 | 7.74e-21 | 87.0 |
| MS.gene07497.t1 | AT1G22590 | 29.012 | 162 | 99 | 3 | 1 | 155 | 1 | 153 | 1.34e-20 | 83.2 |
| MS.gene07497.t1 | AT5G27810 | 42.708 | 96 | 54 | 1 | 29 | 123 | 1 | 96 | 2.55e-20 | 81.3 |
| MS.gene07497.t1 | AT1G31630 | 29.032 | 155 | 108 | 2 | 3 | 156 | 2 | 155 | 3.30e-20 | 85.5 |
| MS.gene07497.t1 | AT1G31640 | 32.000 | 150 | 100 | 2 | 3 | 151 | 2 | 150 | 7.90e-19 | 82.4 |
| MS.gene07497.t1 | AT5G26580 | 36.111 | 144 | 72 | 4 | 1 | 142 | 1 | 126 | 1.68e-17 | 78.2 |
| MS.gene07497.t1 | AT2G40210 | 38.835 | 103 | 58 | 2 | 1 | 100 | 1 | 101 | 1.39e-13 | 67.4 |
| MS.gene07497.t1 | AT5G58890 | 34.234 | 111 | 65 | 3 | 1 | 104 | 1 | 110 | 8.16e-13 | 64.7 |
| MS.gene07497.t1 | AT5G06500 | 29.630 | 162 | 104 | 4 | 1 | 157 | 1 | 157 | 2.57e-12 | 62.8 |
Find 23 sgRNAs with CRISPR-Local
Find 28 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGTTGAATCATTGGTGATAA+AGG | 0.252723 | 1.2:+63080558 | None:intergenic |
| AGCTGAACATTCGTATGATT+TGG | 0.323437 | 1.2:-63080252 | MS.gene07497:CDS |
| GTTGCAAAAGGAAACTTTCC+TGG | 0.331570 | 1.2:+63080332 | None:intergenic |
| AGCCTTTCTTGTTGAATCAT+TGG | 0.370083 | 1.2:+63080549 | None:intergenic |
| CACCAATGATTCAACAAGAA+AGG | 0.413190 | 1.2:-63080551 | MS.gene07497:CDS |
| AAATCTGAAGGAAGCAAAAC+AGG | 0.418438 | 1.2:-63080407 | MS.gene07497:CDS |
| CTTTAGTAATCTGTTGCAAA+AGG | 0.444882 | 1.2:+63080320 | None:intergenic |
| TGTTTCTGATTTGAAAGACT+TGG | 0.473410 | 1.2:-63080194 | MS.gene07497:CDS |
| TCAGAAACAGTCATATCATC+TGG | 0.489973 | 1.2:+63080208 | None:intergenic |
| TTTCACCTTCTTCCTCGTCA+TGG | 0.507566 | 1.2:+63080585 | None:intergenic |
| TGCAACAGATTACTAAAGCT+AGG | 0.518700 | 1.2:-63080315 | MS.gene07497:CDS |
| GCAACAGATTACTAAAGCTA+GGG | 0.523992 | 1.2:-63080314 | MS.gene07497:CDS |
| AGAGAACTCACTATTCTTTG+TGG | 0.526508 | 1.2:-63080487 | MS.gene07497:CDS |
| GAAGAAAAGCATCATCAAGA+AGG | 0.545207 | 1.2:-63080512 | MS.gene07497:CDS |
| GAAGGAAATTGATGACAAGA+TGG | 0.549956 | 1.2:-63080149 | MS.gene07497:CDS |
| TCGGAAATTATAGCACATGC+TGG | 0.559487 | 1.2:+63080460 | None:intergenic |
| TGCAGCCATGACGAGGAAGA+AGG | 0.563607 | 1.2:-63080590 | None:intergenic |
| GAAAGGCTTCCTACAAGAAG+AGG | 0.563850 | 1.2:-63080534 | MS.gene07497:CDS |
| TTCTCCTTGCAGCCATGACG+AGG | 0.566759 | 1.2:-63080597 | None:intergenic |
| AGAGGTGTGGCCAAATCTGA+AGG | 0.570010 | 1.2:-63080419 | MS.gene07497:CDS |
| AAGCAAAACAGGTGATTGAG+AGG | 0.611042 | 1.2:-63080396 | MS.gene07497:CDS |
| TTGATTCTAAAACAGAGGTG+TGG | 0.633348 | 1.2:-63080432 | MS.gene07497:CDS |
| TTGGTACCTGCAAAACAACA+CGG | 0.667106 | 1.2:-63080233 | MS.gene07497:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | GTTAATTTCAAAAAATATGA+AGG | - | chr1.2:63080542-63080561 | MS.gene07497:CDS | 15.0% |
| !!! | TGTTTTAGAATCAAAAGGAT+CGG | + | chr1.2:63080271-63080290 | None:intergenic | 25.0% |
| CTTTAGTAATCTGTTGCAAA+AGG | + | chr1.2:63080392-63080411 | None:intergenic | 30.0% | |
| ! | TGTTGAATCATTGGTGATAA+AGG | + | chr1.2:63080154-63080173 | None:intergenic | 30.0% |
| ! | TGTTTCTGATTTGAAAGACT+TGG | - | chr1.2:63080515-63080534 | MS.gene07497:CDS | 30.0% |
| !!! | ACCTCTGTTTTAGAATCAAA+AGG | + | chr1.2:63080276-63080295 | None:intergenic | 30.0% |
| !!! | TCCTTTTGATTCTAAAACAG+AGG | - | chr1.2:63080272-63080291 | MS.gene07497:CDS | 30.0% |
| AAATCTGAAGGAAGCAAAAC+AGG | - | chr1.2:63080302-63080321 | MS.gene07497:CDS | 35.0% | |
| AGAGAACTCACTATTCTTTG+TGG | - | chr1.2:63080222-63080241 | MS.gene07497:CDS | 35.0% | |
| AGCCTTTCTTGTTGAATCAT+TGG | + | chr1.2:63080163-63080182 | None:intergenic | 35.0% | |
| AGCTGAACATTCGTATGATT+TGG | - | chr1.2:63080457-63080476 | MS.gene07497:CDS | 35.0% | |
| CACCAATGATTCAACAAGAA+AGG | - | chr1.2:63080158-63080177 | MS.gene07497:CDS | 35.0% | |
| GCAACAGATTACTAAAGCTA+GGG | - | chr1.2:63080395-63080414 | MS.gene07497:CDS | 35.0% | |
| TCAGAAACAGTCATATCATC+TGG | + | chr1.2:63080504-63080523 | None:intergenic | 35.0% | |
| TGAGAGAAAAAATGTGAACC+AGG | - | chr1.2:63080359-63080378 | MS.gene07497:CDS | 35.0% | |
| TGCAACAGATTACTAAAGCT+AGG | - | chr1.2:63080394-63080413 | MS.gene07497:CDS | 35.0% | |
| ! | GAAGGAAATTGATGACAAGA+TGG | - | chr1.2:63080560-63080579 | MS.gene07497:CDS | 35.0% |
| ! | TGTTTTGCTTCCTTCAGATT+TGG | + | chr1.2:63080303-63080322 | None:intergenic | 35.0% |
| !! | GAAGAAAAGCATCATCAAGA+AGG | - | chr1.2:63080197-63080216 | MS.gene07497:CDS | 35.0% |
| !! | TTGATTCTAAAACAGAGGTG+TGG | - | chr1.2:63080277-63080296 | MS.gene07497:CDS | 35.0% |
| AAGCAAAACAGGTGATTGAG+AGG | - | chr1.2:63080313-63080332 | MS.gene07497:CDS | 40.0% | |
| GTTGCAAAAGGAAACTTTCC+TGG | + | chr1.2:63080380-63080399 | None:intergenic | 40.0% | |
| TCGGAAATTATAGCACATGC+TGG | + | chr1.2:63080252-63080271 | None:intergenic | 40.0% | |
| TTGGTACCTGCAAAACAACA+CGG | - | chr1.2:63080476-63080495 | MS.gene07497:CDS | 40.0% | |
| ! | GCTTTTCTTCCTCTTCTTGT+AGG | + | chr1.2:63080187-63080206 | None:intergenic | 40.0% |
| GAAAGGCTTCCTACAAGAAG+AGG | - | chr1.2:63080175-63080194 | MS.gene07497:CDS | 45.0% | |
| ! | AGAGGTGTGGCCAAATCTGA+AGG | - | chr1.2:63080290-63080309 | MS.gene07497:CDS | 50.0% |
| !!! | CTGGCACCGTGTTGTTTTGC+AGG | + | chr1.2:63080485-63080504 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.2 | gene | 63080126 | 63080605 | 63080126 | ID=MS.gene07497 |
| chr1.2 | mRNA | 63080126 | 63080605 | 63080126 | ID=MS.gene07497.t1;Parent=MS.gene07497 |
| chr1.2 | exon | 63080126 | 63080605 | 63080126 | ID=MS.gene07497.t1.exon1;Parent=MS.gene07497.t1 |
| chr1.2 | CDS | 63080126 | 63080605 | 63080126 | ID=cds.MS.gene07497.t1;Parent=MS.gene07497.t1 |
| Gene Sequence |
| Protein sequence |
