AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene07540

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07540.t1 MTR_1g078260 71.429 511 55 7 22 499 32 484 0.0 652
MS.gene07540.t1 MTR_6g086555 50.370 405 184 7 21 423 12 401 1.30e-121 362
MS.gene07540.t1 MTR_7g011820 51.852 81 38 1 196 276 387 466 7.45e-22 99.0
MS.gene07540.t1 MTR_7g011820 43.023 86 43 3 219 304 228 307 1.48e-11 67.0
MS.gene07540.t1 MTR_7g071120 34.197 193 108 6 134 312 225 412 1.28e-21 98.2
MS.gene07540.t1 MTR_7g080020 49.474 95 44 3 183 276 458 549 1.46e-21 99.0
MS.gene07540.t1 MTR_3g009470 40.876 137 73 4 158 294 439 567 6.27e-21 96.7
MS.gene07540.t1 MTR_3g104750 52.055 73 34 1 204 276 89 160 9.27e-21 89.7
MS.gene07540.t1 MTR_4g082580 46.875 96 49 2 182 276 240 334 2.06e-20 93.6
MS.gene07540.t1 MTR_4g013260 45.714 105 55 2 172 276 290 392 2.24e-20 94.4
MS.gene07540.t1 MTR_5g094430 37.956 137 81 2 140 276 288 420 2.32e-20 94.7
MS.gene07540.t1 MTR_7g080000 54.930 71 31 1 206 276 419 488 2.52e-20 94.7
MS.gene07540.t1 MTR_5g042920 47.423 97 50 1 201 297 302 397 2.70e-20 93.6
MS.gene07540.t1 MTR_5g094430 37.956 137 81 2 140 276 176 308 4.77e-20 92.8
MS.gene07540.t1 MTR_5g094430 37.956 137 81 2 140 276 154 286 4.98e-20 92.4
MS.gene07540.t1 MTR_1g015140 50.667 75 36 1 202 276 85 158 5.91e-20 87.4
MS.gene07540.t1 MTR_3g095080 52.000 75 35 1 204 278 153 226 6.10e-20 89.4
MS.gene07540.t1 MTR_3g095080 52.000 75 35 1 204 278 129 202 1.02e-19 88.2
MS.gene07540.t1 MTR_8g092010 51.389 72 34 1 205 276 94 164 1.20e-19 87.0
MS.gene07540.t1 MTR_5g074400 52.500 80 37 1 197 276 398 476 1.79e-19 92.0
MS.gene07540.t1 MTR_8g092140 53.521 71 32 1 211 281 152 221 1.96e-19 87.8
MS.gene07540.t1 MTR_3g056100 53.425 73 33 1 204 276 387 458 2.99e-19 91.3
MS.gene07540.t1 MTR_3g056100 53.425 73 33 1 204 276 400 471 3.05e-19 91.3
MS.gene07540.t1 MTR_3g031220 54.795 73 32 1 204 276 350 421 4.41e-19 90.5
MS.gene07540.t1 MTR_3g031220 43.333 90 42 4 219 307 198 279 5.76e-11 65.1
MS.gene07540.t1 MTR_7g109800 41.905 105 48 3 204 307 130 222 5.33e-19 87.8
MS.gene07540.t1 MTR_3g056100 53.425 73 33 1 204 276 243 314 6.03e-19 89.7
MS.gene07540.t1 MTR_3g056100 53.425 73 33 1 204 276 256 327 7.27e-19 89.4
MS.gene07540.t1 MTR_8g007270 48.718 78 39 1 204 281 139 215 1.03e-17 82.8
MS.gene07540.t1 MTR_3g095040 48.000 75 38 1 202 276 101 174 1.15e-17 82.0
MS.gene07540.t1 MTR_4g094588 43.689 103 50 4 206 308 165 259 1.40e-17 84.3
MS.gene07540.t1 MTR_7g079010 50.704 71 34 1 206 276 128 197 1.71e-17 81.6
MS.gene07540.t1 MTR_7g100510 51.351 74 35 1 203 276 345 417 1.93e-17 85.1
MS.gene07540.t1 MTR_4g122530 48.684 76 37 2 206 280 195 269 4.62e-17 83.6
MS.gene07540.t1 MTR_1g086790 49.315 73 36 1 204 276 143 214 6.76e-17 82.0
MS.gene07540.t1 MTR_5g097490 45.333 75 40 1 204 278 237 310 1.02e-16 82.4
MS.gene07540.t1 MTR_7g028415 52.857 70 28 2 209 276 83 149 2.36e-16 77.4
MS.gene07540.t1 MTR_7g028710 52.857 70 28 2 209 276 83 149 2.36e-16 77.4
MS.gene07540.t1 MTR_2g033820 49.315 73 36 1 204 276 188 259 2.85e-16 80.9
MS.gene07540.t1 MTR_7g062220 46.154 78 40 2 200 276 104 180 4.55e-15 76.6
MS.gene07540.t1 MTR_5g043880 45.946 74 39 1 206 279 111 183 5.17e-15 75.9
MS.gene07540.t1 MTR_5g091390 46.479 71 37 1 206 276 144 213 7.34e-15 75.9
MS.gene07540.t1 MTR_1g077780 50.685 73 35 1 204 276 47 118 1.63e-14 71.2
MS.gene07540.t1 MTR_8g096730 44.595 74 40 1 203 276 54 126 2.31e-14 70.9
MS.gene07540.t1 MTR_4g130900 38.462 104 62 2 182 284 321 423 4.60e-14 74.7
MS.gene07540.t1 MTR_7g110720 35.652 115 68 3 167 276 133 246 7.54e-14 73.6
MS.gene07540.t1 MTR_7g110720 38.235 102 57 3 180 276 173 273 8.99e-14 73.6
MS.gene07540.t1 MTR_2g088000 40.909 88 42 3 199 277 321 407 1.20e-13 73.9
MS.gene07540.t1 MTR_4g132430 43.056 72 39 2 206 276 114 184 2.26e-13 72.0
MS.gene07540.t1 MTR_8g028565 39.216 102 45 4 176 276 127 212 5.16e-13 70.5
MS.gene07540.t1 MTR_3g108960 40.594 101 47 4 177 276 283 371 5.39e-13 71.2
MS.gene07540.t1 MTR_4g132430 43.056 72 39 2 206 276 248 318 1.08e-12 70.5
MS.gene07540.t1 MTR_2g045360 27.604 192 127 6 95 276 79 268 1.10e-12 70.1
MS.gene07540.t1 MTR_2g023930 43.056 72 39 2 206 276 292 362 1.31e-12 70.5
MS.gene07540.t1 MTR_3g053330 42.466 73 40 2 206 277 238 309 1.53e-12 70.1
MS.gene07540.t1 MTR_1g009613 38.532 109 47 4 173 276 226 319 1.87e-12 68.9
MS.gene07540.t1 MTR_4g049790 31.061 132 70 4 165 276 239 369 2.19e-12 69.7
MS.gene07540.t1 MTR_2g016770 54.237 59 25 2 220 277 36 93 3.98e-12 66.2
MS.gene07540.t1 MTR_2g016770 55.172 58 24 2 220 276 28 84 5.82e-12 65.5
MS.gene07540.t1 MTR_8g098945 41.837 98 48 4 218 314 128 217 6.57e-12 66.6
MS.gene07540.t1 MTR_3g090860 50.685 73 29 3 209 275 120 191 9.30e-12 66.6
MS.gene07540.t1 MTR_2g105060 40.541 74 40 3 206 276 79 151 1.26e-11 65.1
MS.gene07540.t1 MTR_2g105060 40.541 74 40 3 206 276 154 226 1.36e-11 65.9
MS.gene07540.t1 MTR_4g107970 41.096 73 41 2 206 277 165 236 1.59e-11 67.0
MS.gene07540.t1 MTR_7g109600 51.724 58 26 2 220 276 54 110 1.79e-11 65.1
MS.gene07540.t1 MTR_1g013760 40.000 75 41 3 206 277 125 198 2.08e-11 65.1
MS.gene07540.t1 MTR_4g127040 46.970 66 33 2 216 280 242 306 3.02e-11 65.1
MS.gene07540.t1 MTR_5g016610 47.368 76 34 3 206 276 182 256 3.04e-11 64.3
MS.gene07540.t1 MTR_3g031220 43.333 90 42 4 219 307 198 279 3.10e-11 65.5
MS.gene07540.t1 MTR_1g070455 49.231 65 31 2 216 279 290 353 3.16e-11 65.5
MS.gene07540.t1 MTR_4g107970 41.096 73 41 2 206 277 242 313 3.24e-11 66.2
MS.gene07540.t1 MTR_0117s0040 49.254 67 32 2 216 281 71 136 3.44e-11 63.9
MS.gene07540.t1 MTR_2g032030 45.455 66 34 2 212 276 260 324 4.93e-11 65.1
MS.gene07540.t1 MTR_4g049760 41.558 77 43 2 201 276 272 347 5.25e-11 65.5
MS.gene07540.t1 MTR_7g009730 43.056 72 39 2 206 276 206 276 5.42e-11 65.5
MS.gene07540.t1 MTR_4g094908 49.206 63 30 2 216 277 263 324 7.83e-11 63.9
MS.gene07540.t1 MTR_7g079040 49.206 63 30 2 215 276 150 211 9.71e-11 63.5
MS.gene07540.t1 MTR_2g032030 45.455 66 34 2 212 276 260 324 9.96e-11 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene07540.t1 AT5G64810 38.835 103 62 1 174 276 65 166 2.60e-21 92.0
MS.gene07540.t1 AT1G64000 55.556 72 31 1 205 276 100 170 3.02e-21 92.0
MS.gene07540.t1 AT2G38470 41.538 130 70 3 149 276 293 418 6.06e-21 96.3
MS.gene07540.t1 AT4G26640 42.609 115 54 2 173 276 284 397 1.49e-20 95.1
MS.gene07540.t1 AT5G41570 54.930 71 31 1 206 276 85 154 1.87e-20 89.4
MS.gene07540.t1 AT4G26640 42.609 115 54 2 173 276 252 365 2.51e-20 94.4
MS.gene07540.t1 AT5G07100 52.381 84 32 2 204 280 219 301 2.63e-20 92.0
MS.gene07540.t1 AT4G26640 42.609 115 54 2 173 276 324 437 2.66e-20 94.4
MS.gene07540.t1 AT1G13960 53.947 76 34 1 201 276 391 465 3.49e-20 94.0
MS.gene07540.t1 AT4G26640 54.795 73 32 1 204 276 190 261 3.55e-20 92.8
MS.gene07540.t1 AT4G26640 54.795 73 32 1 204 276 190 261 3.55e-20 92.8
MS.gene07540.t1 AT4G26640 54.795 73 32 1 204 276 190 261 3.55e-20 92.8
MS.gene07540.t1 AT4G26640 54.795 73 32 1 204 276 190 261 3.55e-20 92.8
MS.gene07540.t1 AT5G07100 52.381 84 32 2 204 280 126 208 3.72e-20 89.4
MS.gene07540.t1 AT5G07100 52.381 84 32 2 204 280 126 208 3.72e-20 89.4
MS.gene07540.t1 AT5G07100 52.381 84 32 2 204 280 126 208 3.72e-20 89.4
MS.gene07540.t1 AT5G07100 52.381 84 32 2 204 280 126 208 3.72e-20 89.4
MS.gene07540.t1 AT2G03340 53.947 76 34 1 201 276 397 471 3.95e-20 94.0
MS.gene07540.t1 AT3G01080 53.947 76 34 1 201 276 326 400 4.52e-20 93.2
MS.gene07540.t1 AT3G01080 53.947 76 34 1 201 276 288 362 8.51e-20 92.0
MS.gene07540.t1 AT1G13960 53.947 76 34 1 201 276 238 312 1.07e-19 90.9
MS.gene07540.t1 AT4G26440 44.860 107 56 2 170 276 325 428 2.42e-19 91.7
MS.gene07540.t1 AT5G56270 54.795 73 32 1 204 276 472 543 3.42e-19 91.3
MS.gene07540.t1 AT1G55600 52.632 76 35 1 201 276 289 363 1.76e-18 88.6
MS.gene07540.t1 AT2G04880 38.017 121 74 1 156 276 220 339 2.01e-18 88.2
MS.gene07540.t1 AT2G04880 38.017 121 74 1 156 276 244 363 2.83e-18 87.8
MS.gene07540.t1 AT2G44745 46.429 84 44 1 204 287 130 212 6.31e-18 83.2
MS.gene07540.t1 AT5G49520 52.055 73 34 1 204 276 206 277 7.31e-18 85.9
MS.gene07540.t1 AT2G30250 52.055 73 34 1 204 276 313 384 7.51e-18 85.9
MS.gene07540.t1 AT2G46130 52.113 71 33 1 206 276 17 86 1.09e-17 79.3
MS.gene07540.t1 AT2G47260 49.315 73 36 1 204 276 159 230 1.18e-17 84.7
MS.gene07540.t1 AT4G39410 49.333 75 37 1 204 278 208 281 2.22e-17 83.2
MS.gene07540.t1 AT5G26170 43.243 74 41 1 206 279 100 172 2.40e-17 80.1
MS.gene07540.t1 AT1G29860 49.315 73 36 1 204 276 121 192 2.58e-17 82.8
MS.gene07540.t1 AT4G18170 47.945 73 37 1 204 276 157 228 2.21e-16 80.5
MS.gene07540.t1 AT1G69310 45.946 74 39 1 206 279 134 206 4.80e-16 79.0
MS.gene07540.t1 AT1G69310 45.946 74 39 1 206 279 134 206 4.80e-16 79.0
MS.gene07540.t1 AT1G69310 45.946 74 39 1 206 279 134 206 4.80e-16 79.0
MS.gene07540.t1 AT3G62340 52.113 71 33 1 206 276 105 174 6.44e-16 78.6
MS.gene07540.t1 AT5G13080 48.611 72 36 1 205 276 53 123 8.45e-16 75.1
MS.gene07540.t1 AT5G46350 45.070 71 38 1 206 276 170 239 2.26e-15 77.8
MS.gene07540.t1 AT3G01970 48.052 77 37 2 206 282 52 125 2.70e-15 73.6
MS.gene07540.t1 AT2G21900 51.562 64 29 2 214 276 103 165 3.29e-15 74.7
MS.gene07540.t1 AT2G46130 47.887 71 32 1 206 276 17 82 5.17e-15 71.2
MS.gene07540.t1 AT4G31550 37.903 124 58 5 174 296 211 316 6.45e-15 76.3
MS.gene07540.t1 AT1G69310 41.892 74 38 1 206 279 134 202 7.99e-14 72.4
MS.gene07540.t1 AT4G30935 39.252 107 55 3 182 284 295 395 1.54e-13 73.2
MS.gene07540.t1 AT4G31550 37.600 125 58 6 174 296 211 317 2.21e-13 71.6
MS.gene07540.t1 AT2G37260 49.206 63 31 1 214 276 344 405 2.52e-13 72.4
MS.gene07540.t1 AT2G37260 49.206 63 31 1 214 276 264 325 2.89e-13 71.6
MS.gene07540.t1 AT2G37260 49.206 63 31 1 214 276 262 323 2.98e-13 71.6
MS.gene07540.t1 AT4G01250 41.000 100 43 4 178 276 101 185 5.59e-13 70.1
MS.gene07540.t1 AT4G23550 43.243 74 40 2 204 276 125 197 1.07e-12 69.3
MS.gene07540.t1 AT4G04450 44.444 72 38 2 206 276 185 255 1.22e-12 70.1
MS.gene07540.t1 AT5G52830 39.623 106 56 3 204 308 150 248 1.43e-12 69.3
MS.gene07540.t1 AT2G25000 34.862 109 55 5 185 278 56 163 2.95e-12 66.6
MS.gene07540.t1 AT2G25000 34.862 109 55 5 185 278 56 163 2.95e-12 66.6
MS.gene07540.t1 AT1G29280 43.011 93 48 3 185 276 43 131 3.04e-12 67.4
MS.gene07540.t1 AT2G46400 45.205 73 36 2 206 275 91 162 3.45e-12 67.8
MS.gene07540.t1 AT4G24240 44.444 81 39 3 206 281 264 343 3.46e-12 68.2
MS.gene07540.t1 AT4G04450 44.444 72 38 2 206 276 279 349 3.47e-12 68.9
MS.gene07540.t1 AT4G22070 43.056 72 39 2 206 276 284 354 4.40e-12 68.9
MS.gene07540.t1 AT4G22070 43.056 72 39 2 206 276 284 354 4.40e-12 68.9
MS.gene07540.t1 AT4G22070 43.056 72 39 2 206 276 284 354 4.40e-12 68.9
MS.gene07540.t1 AT4G22070 43.056 72 39 2 206 276 284 354 4.40e-12 68.9
MS.gene07540.t1 AT2G23320 43.750 80 39 3 203 277 220 298 4.50e-12 67.4
MS.gene07540.t1 AT2G25000 34.862 109 55 5 185 278 78 185 4.56e-12 66.6
MS.gene07540.t1 AT5G15130 43.056 72 39 2 206 276 172 242 6.73e-12 68.2
MS.gene07540.t1 AT2G25000 34.862 109 55 5 185 278 99 206 7.73e-12 66.2
MS.gene07540.t1 AT5G15130 43.056 72 39 2 206 276 214 284 8.58e-12 67.8
MS.gene07540.t1 AT1G62300 43.056 72 39 2 206 276 299 369 9.86e-12 67.8
MS.gene07540.t1 AT5G43290 43.056 72 39 2 206 276 100 170 1.05e-11 65.9
MS.gene07540.t1 AT4G01720 44.444 72 38 2 206 276 226 296 1.09e-11 67.4
MS.gene07540.t1 AT2G24570 40.000 105 49 5 174 276 208 300 2.19e-11 65.5
MS.gene07540.t1 AT2G34830 47.692 65 32 2 213 276 209 272 4.66e-11 65.1
MS.gene07540.t1 AT2G34830 47.692 65 32 2 213 276 218 281 5.82e-11 65.1

Find 133 sgRNAs with CRISPR-Local

Find 202 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCTCAAGTCTTTCCTTTCA+TGG 0.213204 1.2:-62415075 MS.gene07540:CDS
TGAAAGTGATGAAGACATTT+TGG 0.222211 1.2:-62416580 MS.gene07540:CDS
ATCAGTTGTTCTATCTTCTT+TGG 0.239892 1.2:+62416933 None:intergenic
CAAGTTGGACCTAATATTAA+TGG 0.252073 1.2:-62415203 MS.gene07540:CDS
AATAATTACCTACGATTGTT+AGG 0.259815 1.2:+62416507 None:intergenic
GGAATTGGCCCGTTGATATT+TGG 0.273233 1.2:+62415368 None:intergenic
AATGAATCTCCTCCTTCTTC+TGG 0.277307 1.2:-62418298 MS.gene07540:CDS
GAAATTGACCCATTAATATT+AGG 0.309554 1.2:+62415194 None:intergenic
TATGTAACAAGTACATATTT+TGG 0.310515 1.2:+62415716 None:intergenic
CCAACTTGAGTTGTGGTAAT+AGG 0.312353 1.2:+62415218 None:intergenic
TTTCTTCAGGTTGGTGGAAA+TGG 0.328210 1.2:-62415107 MS.gene07540:CDS
AAAGGCCGCGTCATGGTATT+AGG 0.337576 1.2:+62415497 None:intergenic
TTACCCACCATGTTTAATAA+AGG 0.347175 1.2:+62415479 None:intergenic
GGGTGCTTCCCCAGCTGATC+AGG 0.355314 1.2:-62415054 MS.gene07540:CDS
GTCCTTGATCAGGTGGTGTT+GGG 0.367401 1.2:+62417076 None:intergenic
AACTCCTATTGATACAAATC+AGG 0.367991 1.2:-62415396 MS.gene07540:CDS
ATACCTTATGGTATGAATTA+CGG 0.368502 1.2:-62415524 MS.gene07540:CDS
CAATATGGCTATATACCTTA+TGG 0.368992 1.2:-62415536 MS.gene07540:CDS
CATAAGGTATATAGCCATAT+TGG 0.374743 1.2:+62415537 None:intergenic
GCTCAAGTCTTTCCTTTCAT+GGG 0.380582 1.2:-62415074 MS.gene07540:CDS
TCGAGAAATATTAATGCATC+TGG 0.384031 1.2:-62415641 MS.gene07540:CDS
GGAAATACAGGAAGCGGAAT+TGG 0.386630 1.2:+62415353 None:intergenic
CCTAAATATCATTCGGTGTC+AGG 0.391994 1.2:+62415017 None:intergenic
TGCAATTCAGGAAGAGAAAG+AGG 0.396225 1.2:-62416671 MS.gene07540:intron
CCATTTCACATTGTACCATA+TGG 0.401565 1.2:-62415137 MS.gene07540:CDS
TATGTACTTGTTACATATGA+TGG 0.402941 1.2:-62415710 MS.gene07540:CDS
CCAACTTGAGTTGCAGTCAT+AGG 0.404757 1.2:+62415305 None:intergenic
TGTTCACTTATGGGTCTGTA+AGG 0.409849 1.2:+62415443 None:intergenic
TCTGCACCATGTCCTTGATC+AGG 0.410286 1.2:+62417066 None:intergenic
GTTGTTGTTGTTATTGGTGT+TGG 0.410712 1.2:+62418399 None:intergenic
GGTGGTAATAGTGGTGATAT+CGG 0.419782 1.2:+62415158 None:intergenic
CAAGTTGGACATAATATCAA+TGG 0.426656 1.2:-62415290 MS.gene07540:CDS
CCAACCTGATTTGTATCAAT+AGG 0.427285 1.2:+62415392 None:intergenic
GGTGGTAATGGTGGTGATAT+CGG 0.428089 1.2:+62415245 None:intergenic
GGTGGTAATGGTGGTGATAT+CGG 0.428089 1.2:+62415332 None:intergenic
TCCGTAGCTTCCTGATCAGC+TGG 0.428219 1.2:+62415044 None:intergenic
AAGTGATTATAAAACCTTTG+TGG 0.432121 1.2:+62416787 None:intergenic
TATAGCCATATTGGTGAAAA+GGG 0.437091 1.2:+62415546 None:intergenic
ATATAGCCATATTGGTGAAA+AGG 0.444538 1.2:+62415545 None:intergenic
CATCTCCTCTTGTTGCCACA+TGG 0.452151 1.2:+62417151 None:intergenic
AAGTTGGACCTAATATTAAT+GGG 0.454569 1.2:-62415202 MS.gene07540:CDS
GGTGGTGATATCGGAAATAC+AGG 0.454748 1.2:+62415254 None:intergenic
GGTGGTGATATCGGAAATAC+AGG 0.454748 1.2:+62415341 None:intergenic
CACACATATGTCTAATATCA+TGG 0.457002 1.2:-62418434 None:intergenic
AGTGGTGATATCGGAAATAC+AGG 0.457187 1.2:+62415167 None:intergenic
AATGCAGGTGGTGGTGATGG+TGG 0.472619 1.2:+62415590 None:intergenic
TGAGTTGCAGTCATAGGAAA+TGG 0.474839 1.2:+62415311 None:intergenic
ACTGCTTCAGAAAAGTTCAT+TGG 0.480463 1.2:+62416961 None:intergenic
CAACCGTAATTCATACCATA+AGG 0.481218 1.2:+62415521 None:intergenic
CCTATGACTGCAACTCAAGT+TGG 0.482132 1.2:-62415305 MS.gene07540:CDS
TGTCCTTGATCAGGTGGTGT+TGG 0.482577 1.2:+62417075 None:intergenic
GTAATAGGAAATGGTGGTAA+TGG 0.484085 1.2:+62415233 None:intergenic
CCTATTACCACAACTCAAGT+TGG 0.484916 1.2:-62415218 MS.gene07540:CDS
GCAGGTGGTGGTGATGGTGG+TGG 0.485365 1.2:+62415593 None:intergenic
TGAAGAAAATCCCCACCATA+TGG 0.492962 1.2:+62415122 None:intergenic
ACCACCACCTGCATTGCCTA+TGG 0.498003 1.2:-62415582 MS.gene07540:CDS
TTAATATTTCTCGAGTATGT+TGG 0.504174 1.2:+62415650 None:intergenic
GATGATGAAGCAACTGCCAT+TGG 0.509238 1.2:-62416859 MS.gene07540:CDS
TATGTAGCCTAAATATCATT+CGG 0.509419 1.2:+62415010 None:intergenic
GGGTGAACCATAGGCAATGC+AGG 0.510944 1.2:+62415575 None:intergenic
GGCAATGCAGGTGGTGGTGA+TGG 0.511381 1.2:+62415587 None:intergenic
AGGTTGGACCAAATATCAAC+GGG 0.512849 1.2:-62415376 MS.gene07540:CDS
CAAGTATCGAGCACTGGAGT+TGG 0.518387 1.2:-62418364 MS.gene07540:CDS
TTCACATTGTACCATATGGT+GGG 0.526608 1.2:-62415133 MS.gene07540:CDS
CCATATGGTACAATGTGAAA+TGG 0.527122 1.2:+62415137 None:intergenic
GCTGGGGAAGCACCCATGAA+AGG 0.527399 1.2:+62415062 None:intergenic
CCCAGCTGATCAGGAAGCTA+CGG 0.531217 1.2:-62415045 MS.gene07540:CDS
TGAGTTGTGGTAATAGGAAA+TGG 0.532382 1.2:+62415224 None:intergenic
ATGTGAAATGGTGGTAATAG+TGG 0.532968 1.2:+62415149 None:intergenic
AAGTTGGACATAATATCAAT+GGG 0.540013 1.2:-62415289 MS.gene07540:CDS
TCCCCAACACCACCTGATCA+AGG 0.540123 1.2:-62417078 MS.gene07540:CDS
TATGATGATGGTGGTGCTAG+TGG 0.544707 1.2:-62416817 MS.gene07540:CDS
TCGACATCTGTCATGGGGCA+AGG 0.545512 1.2:+62416624 None:intergenic
GAAAATCAAGTATCGAGCAC+TGG 0.550480 1.2:-62418370 MS.gene07540:CDS
GTCATAGGAAATGGTGGTAA+TGG 0.551917 1.2:+62415320 None:intergenic
GATGAAGCAACTGCCATTGG+AGG 0.552119 1.2:-62416856 MS.gene07540:CDS
GGTTATTGTTGGCGCAAGTA+CGG 0.553147 1.2:-62416552 MS.gene07540:CDS
AAATTCTAAGGGCAAGGAAA+GGG 0.559579 1.2:-62416990 MS.gene07540:CDS
TCACATTGTACCATATGGTG+GGG 0.559672 1.2:-62415132 MS.gene07540:CDS
GAAATTCTAAGGGCAAGGAA+AGG 0.560612 1.2:-62416991 MS.gene07540:CDS
ATGCTGAAATTCTAAGGGCA+AGG 0.561694 1.2:-62416996 MS.gene07540:CDS
CCGTAGCTTCCTGATCAGCT+GGG 0.562306 1.2:+62415045 None:intergenic
TCCTTCTTCTGGTTATTCCG+AGG 0.562652 1.2:-62418287 MS.gene07540:intron
ACCTCGGAATAACCAGAAGA+AGG 0.563675 1.2:+62418286 None:intergenic
GATATCGGAAATACAGGAAG+CGG 0.565608 1.2:+62415347 None:intergenic
ATAGGAAATGGTGGTAATGG+TGG 0.566411 1.2:+62415236 None:intergenic
CAGCAACATCATTCCTCCAA+TGG 0.568839 1.2:+62416843 None:intergenic
TTTGTCAATGACAGGTCCGT+GGG 0.568911 1.2:+62415679 None:intergenic
TTTCACATTGTACCATATGG+TGG 0.572755 1.2:-62415134 MS.gene07540:CDS
GATATCGGAAATACAGGAAG+CGG 0.574632 1.2:+62415260 None:intergenic
CCTATTGATACAAATCAGGT+TGG 0.576330 1.2:-62415392 MS.gene07540:CDS
GCAATTCAGGAAGAGAAAGA+GGG 0.576835 1.2:-62416670 MS.gene07540:intron
TCCTTGATCAGGTGGTGTTG+GGG 0.585344 1.2:+62417077 None:intergenic
GTTTAATAAAGGCCGCGTCA+TGG 0.585854 1.2:+62415490 None:intergenic
GCAGTTGCTTCATCATCCGC+AGG 0.586772 1.2:+62416865 None:intergenic
ATTAGGTCCAACTTGAGTTG+TGG 0.589594 1.2:+62415211 None:intergenic
TGTCTTCATCACTTTCAACT+CGG 0.590497 1.2:+62416586 None:intergenic
CTTATGGTATGAATTACGGT+TGG 0.590836 1.2:-62415520 MS.gene07540:CDS
TGATGGTGGTGGCAGCATGT+TGG 0.595809 1.2:+62415604 None:intergenic
CGGCCTTTATTAAACATGGT+GGG 0.600743 1.2:-62415482 MS.gene07540:CDS
CAGGTTGGACCAAATATCAA+CGG 0.601960 1.2:-62415377 MS.gene07540:CDS
ACCATAGGCAATGCAGGTGG+TGG 0.608469 1.2:+62415581 None:intergenic
TACGGATGCAAAGAGATCAA+AGG 0.609552 1.2:-62416534 MS.gene07540:CDS
GCACCATGTCCTTGATCAGG+TGG 0.613784 1.2:+62417069 None:intergenic
GACGCGGCCTTTATTAAACA+TGG 0.614916 1.2:-62415486 MS.gene07540:CDS
TCTGAAACTTGATCATCAGT+TGG 0.615285 1.2:+62416892 None:intergenic
AAGGAAATCCTAACAATCGT+AGG 0.617006 1.2:-62416515 MS.gene07540:intron
TTTGCTCGACATCTGTCATG+GGG 0.618862 1.2:+62416619 None:intergenic
TAGCCATATTGGTGAAAAGG+GGG 0.626106 1.2:+62415548 None:intergenic
ATGTTGCTGCAATATGATGA+TGG 0.628176 1.2:-62416829 MS.gene07540:CDS
ATAGGAAATGGTGGTAATGG+TGG 0.629683 1.2:+62415323 None:intergenic
GCGGCCTTTATTAAACATGG+TGG 0.630164 1.2:-62415483 MS.gene07540:CDS
GATTGCCATGTGGCAACAAG+AGG 0.647237 1.2:-62417156 MS.gene07540:CDS
TTGGTTGCTCTGCTAAACAA+AGG 0.648861 1.2:-62418345 MS.gene07540:CDS
CGTAGCTTCCTGATCAGCTG+GGG 0.653265 1.2:+62415046 None:intergenic
TATGATGGAAAACATACCCA+CGG 0.656713 1.2:-62415695 MS.gene07540:CDS
GGGTCTGTAAGGTTGCATCA+TGG 0.657757 1.2:+62415454 None:intergenic
GTTGCAGTCATAGGAAATGG+TGG 0.663032 1.2:+62415314 None:intergenic
GCAAAATGATGCAAATGTAG+TGG 0.669148 1.2:-62417025 MS.gene07540:CDS
ATAGCCATATTGGTGAAAAG+GGG 0.680391 1.2:+62415547 None:intergenic
CTAGTGGATCTTGTCCACAA+AGG 0.681150 1.2:-62416801 MS.gene07540:intron
ACATCTGTCATGGGGCAAGG+TGG 0.684322 1.2:+62416627 None:intergenic
GGGGATGAGAAATTCAACAC+TGG 0.684419 1.2:+62417096 None:intergenic
GTTGTGGTAATAGGAAATGG+TGG 0.688257 1.2:+62415227 None:intergenic
TCGGAATAACCAGAAGAAGG+AGG 0.688798 1.2:+62418289 None:intergenic
TTCTGCTGATGATTGCCATG+TGG 0.692089 1.2:-62417166 MS.gene07540:CDS
TATGGTACAATGTGAAATGG+TGG 0.694153 1.2:+62415140 None:intergenic
ACACCACCTGATCAAGGACA+TGG 0.702867 1.2:-62417072 MS.gene07540:CDS
TGAACCATAGGCAATGCAGG+TGG 0.713852 1.2:+62415578 None:intergenic
AGCCATATTGGTGAAAAGGG+GGG 0.719943 1.2:+62415549 None:intergenic
TTGCTGCAATATGATGATGG+TGG 0.726113 1.2:-62416826 MS.gene07540:CDS
TCAAGTTTCAGATACACCTG+CGG 0.763156 1.2:-62416881 MS.gene07540:CDS
GTTGGCCTAATACCATGACG+CGG 0.807585 1.2:-62415502 MS.gene07540:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TGACATTATTTAAAAAAAAA+GGG + chr1.2:62415729-62415748 None:intergenic 10.0%
!!! TTTTCATCAAAAAATAAAAA+AGG + chr1.2:62415420-62415439 None:intergenic 10.0%
!! AGAATTTGATATTAAATTTG+TGG - chr1.2:62417405-62417424 MS.gene07540:intron 15.0%
!! CAAATTTAATATCAAATTCT+AGG + chr1.2:62417405-62417424 None:intergenic 15.0%
!! GTGACATTATTTAAAAAAAA+AGG + chr1.2:62415730-62415749 None:intergenic 15.0%
!! TACTAATACATTTATGTAAA+TGG + chr1.2:62417579-62417598 None:intergenic 15.0%
!! TCACACAATATTAAATAATT+AGG + chr1.2:62417642-62417661 None:intergenic 15.0%
!! TTGTAAAAATATCAAAAACA+AGG + chr1.2:62417248-62417267 None:intergenic 15.0%
!!! CTTTATCATTTTATTTTAAG+CGG - chr1.2:62417475-62417494 MS.gene07540:intron 15.0%
!!! TTTTGTTTTTATAGTTTCAT+AGG - chr1.2:62416084-62416103 MS.gene07540:intron 15.0%
!! ACATTGTTGCTAATAAATAA+AGG - chr1.2:62416733-62416752 MS.gene07540:intron 20.0%
!! ATAAATAAATCATTCCATGT+TGG + chr1.2:62416138-62416157 None:intergenic 20.0%
!! ATATTCCTAATGAGAAATAA+TGG + chr1.2:62415881-62415900 None:intergenic 20.0%
!! GTAATTGAATCAAATTCATT+AGG - chr1.2:62416262-62416281 MS.gene07540:intron 20.0%
!! TACATGTTAGATTGAATTAA+AGG + chr1.2:62415980-62415999 None:intergenic 20.0%
!! TCTTCATTCAAGATTTAAAA+TGG + chr1.2:62415691-62415710 None:intergenic 20.0%
!! TTAATATTGTGTGAATTGTA+GGG - chr1.2:62417648-62417667 MS.gene07540:intron 20.0%
!! TTTAATATTGTGTGAATTGT+AGG - chr1.2:62417647-62417666 MS.gene07540:intron 20.0%
!!! AAAAGAGTATATAAATACCT+CGG + chr1.2:62415185-62415204 None:intergenic 20.0%
!!! AATGGCTTTTCAATTAAATT+CGG + chr1.2:62415673-62415692 None:intergenic 20.0%
!!! AGAAAATTTTGTTCACTTAT+GGG + chr1.2:62418021-62418040 None:intergenic 20.0%
!!! TAGAAAATTTTGTTCACTTA+TGG + chr1.2:62418022-62418041 None:intergenic 20.0%
!!! TATGTAACAAGTACATATTT+TGG + chr1.2:62417739-62417758 None:intergenic 20.0%
!!! TTTTACGATAAATCACTTAT+CGG + chr1.2:62417378-62417397 None:intergenic 20.0%
! AAGTGATTATAAAACCTTTG+TGG + chr1.2:62416668-62416687 None:intergenic 25.0%
! AAGTTGGACATAATATCAAT+GGG - chr1.2:62418163-62418182 MS.gene07540:intron 25.0%
! AAGTTGGACCTAATATTAAT+GGG - chr1.2:62418250-62418269 MS.gene07540:intron 25.0%
! AAGTTGTATTAGTGCTAAAA+TGG + chr1.2:62415913-62415932 None:intergenic 25.0%
! AATAATTACCTACGATTGTT+AGG + chr1.2:62416948-62416967 None:intergenic 25.0%
! AATCTAACAATGATTGATGT+TGG + chr1.2:62417315-62417334 None:intergenic 25.0%
! AATGACATAATGATCATTGA+CGG - chr1.2:62417186-62417205 MS.gene07540:intron 25.0%
! ACATTAAAAAACAAGTGGAA+AGG - chr1.2:62417701-62417720 MS.gene07540:intron 25.0%
! ATACCTTATGGTATGAATTA+CGG - chr1.2:62417928-62417947 MS.gene07540:intron 25.0%
! ATTGAATTAAAGGTCTGATT+AGG + chr1.2:62415970-62415989 None:intergenic 25.0%
! CATTAAAAAACAAGTGGAAA+GGG - chr1.2:62417702-62417721 MS.gene07540:intron 25.0%
! GAAAAATGCTGAAATTCTAA+GGG - chr1.2:62416451-62416470 MS.gene07540:intron 25.0%
! GAAATTGACCCATTAATATT+AGG + chr1.2:62418261-62418280 None:intergenic 25.0%
! GAATTTGGATTCGTTTATAT+TGG + chr1.2:62415802-62415821 None:intergenic 25.0%
! TAATATTGTGTGAATTGTAG+GGG - chr1.2:62417649-62417668 MS.gene07540:intron 25.0%
! TGAAAAATGCTGAAATTCTA+AGG - chr1.2:62416450-62416469 MS.gene07540:intron 25.0%
! TGCATTAACACATATAAAAG+AGG + chr1.2:62416973-62416992 None:intergenic 25.0%
! TTAATATTTCTCGAGTATGT+TGG + chr1.2:62417805-62417824 None:intergenic 25.0%
! TTGTGACATTAAAAAACAAG+TGG - chr1.2:62417696-62417715 MS.gene07540:intron 25.0%
!! ATTGTTGTTGTTGTTGTTAT+TGG + chr1.2:62415062-62415081 None:intergenic 25.0%
!! TATGTACTTGTTACATATGA+TGG - chr1.2:62417742-62417761 MS.gene07540:intron 25.0%
!!! AGAGATGATTTTCAAGTATT+GGG - chr1.2:62417336-62417355 MS.gene07540:intron 25.0%
!!! TAGAGATGATTTTCAAGTAT+TGG - chr1.2:62417335-62417354 MS.gene07540:intron 25.0%
AACTCCTATTGATACAAATC+AGG - chr1.2:62418056-62418075 MS.gene07540:intron 30.0%
AATATTGTGTGAATTGTAGG+GGG - chr1.2:62417650-62417669 MS.gene07540:intron 30.0%
ATATAGCCATATTGGTGAAA+AGG + chr1.2:62417910-62417929 None:intergenic 30.0%
ATCAGTTGTTCTATCTTCTT+TGG + chr1.2:62416522-62416541 None:intergenic 30.0%
CAATATGGCTATATACCTTA+TGG - chr1.2:62417916-62417935 MS.gene07540:intron 30.0%
CATAAGGTATATAGCCATAT+TGG + chr1.2:62417918-62417937 None:intergenic 30.0%
GGTGAAAAATGATGTGAATT+TGG + chr1.2:62415817-62415836 None:intergenic 30.0%
GTCAATGATCATTATGTCAT+TGG + chr1.2:62417187-62417206 None:intergenic 30.0%
TATAGCCATATTGGTGAAAA+GGG + chr1.2:62417909-62417928 None:intergenic 30.0%
TCGAGAAATATTAATGCATC+TGG - chr1.2:62417811-62417830 MS.gene07540:intron 30.0%
TTACCCACCATGTTTAATAA+AGG + chr1.2:62417976-62417995 None:intergenic 30.0%
! ACTTTTAATAACCACACAAG+CGG + chr1.2:62415843-62415862 None:intergenic 30.0%
! ATGCACCATTATTTCTCATT+AGG - chr1.2:62415873-62415892 MS.gene07540:intron 30.0%
! CAAGTTGGACATAATATCAA+TGG - chr1.2:62418162-62418181 MS.gene07540:intron 30.0%
! CAAGTTGGACCTAATATTAA+TGG - chr1.2:62418249-62418268 MS.gene07540:intron 30.0%
! TGAAAGTGATGAAGACATTT+TGG - chr1.2:62416872-62416891 MS.gene07540:CDS 30.0%
!! CAAATAGTTTTACACCAACA+TGG - chr1.2:62416121-62416140 MS.gene07540:intron 30.0%
!! GCTCAATTATTTTGCAATTC+AGG - chr1.2:62416769-62416788 MS.gene07540:intron 30.0%
!! TTTTCTTTTTCATTCACTCC+TGG - chr1.2:62417016-62417035 MS.gene07540:CDS 30.0%
!! TTTTGGATGATGGTTATTGT+TGG - chr1.2:62416889-62416908 MS.gene07540:CDS 30.0%
AAGGAAATCCTAACAATCGT+AGG - chr1.2:62416937-62416956 MS.gene07540:CDS 35.0%
ACAACAGTGTTCATCATCAT+TGG + chr1.2:62417118-62417137 None:intergenic 35.0%
ACTGCTTCAGAAAAGTTCAT+TGG + chr1.2:62416494-62416513 None:intergenic 35.0%
ATAGCCATATTGGTGAAAAG+GGG + chr1.2:62417908-62417927 None:intergenic 35.0%
ATGTGAAATGGTGGTAATAG+TGG + chr1.2:62418306-62418325 None:intergenic 35.0%
ATGTTGCTGCAATATGATGA+TGG - chr1.2:62416623-62416642 MS.gene07540:CDS 35.0%
CAACCGTAATTCATACCATA+AGG + chr1.2:62417934-62417953 None:intergenic 35.0%
CCATATGGTACAATGTGAAA+TGG + chr1.2:62418318-62418337 None:intergenic 35.0%
CCATTTCACATTGTACCATA+TGG - chr1.2:62418315-62418334 MS.gene07540:CDS 35.0%
CCTATTGATACAAATCAGGT+TGG - chr1.2:62418060-62418079 MS.gene07540:intron 35.0%
CTTATGGTATGAATTACGGT+TGG - chr1.2:62417932-62417951 MS.gene07540:intron 35.0%
GCAAAATGATGCAAATGTAG+TGG - chr1.2:62416427-62416446 MS.gene07540:intron 35.0%
GTAATAGGAAATGGTGGTAA+TGG + chr1.2:62418222-62418241 None:intergenic 35.0%
TATGGTACAATGTGAAATGG+TGG + chr1.2:62418315-62418334 None:intergenic 35.0%
TCCTCTCTATATATACACCA+AGG + chr1.2:62417042-62417061 None:intergenic 35.0%
TGAGTTGTGGTAATAGGAAA+TGG + chr1.2:62418231-62418250 None:intergenic 35.0%
TGTCTTCATCACTTTCAACT+CGG + chr1.2:62416869-62416888 None:intergenic 35.0%
TTCACATTGTACCATATGGT+GGG - chr1.2:62418319-62418338 MS.gene07540:CDS 35.0%
TTGGTGTATATATAGAGAGG+AGG - chr1.2:62417041-62417060 MS.gene07540:CDS 35.0%
TTTCACATTGTACCATATGG+TGG - chr1.2:62418318-62418337 MS.gene07540:CDS 35.0%
! AAATTCTAAGGGCAAGGAAA+GGG - chr1.2:62416462-62416481 MS.gene07540:intron 35.0%
! CATTTGCATCATTTTGCACA+TGG + chr1.2:62416424-62416443 None:intergenic 35.0%
! CCAACCTGATTTGTATCAAT+AGG + chr1.2:62418063-62418082 None:intergenic 35.0%
! GATGAAGACATTTTGGATGA+TGG - chr1.2:62416879-62416898 MS.gene07540:CDS 35.0%
! GTTGTTGTTGTTATTGGTGT+TGG + chr1.2:62415056-62415075 None:intergenic 35.0%
! TATGATGGAAAACATACCCA+CGG - chr1.2:62417757-62417776 MS.gene07540:intron 35.0%
! TCTGAAACTTGATCATCAGT+TGG + chr1.2:62416563-62416582 None:intergenic 35.0%
!! TGATTGATGTTGGTTCAACA+CGG + chr1.2:62417305-62417324 None:intergenic 35.0%
!! TTATGTCATTGGCTTAGTTG+TGG + chr1.2:62417176-62417195 None:intergenic 35.0%
!!! GGTCTTTTTTTGTCAATGAC+AGG + chr1.2:62417784-62417803 None:intergenic 35.0%
AAATGAACCAGTAGCAACCA+CGG + chr1.2:62415597-62415616 None:intergenic 40.0%
AATGAATCTCCTCCTTCTTC+TGG - chr1.2:62415154-62415173 MS.gene07540:CDS 40.0%
AGGTTGGACCAAATATCAAC+GGG - chr1.2:62418076-62418095 MS.gene07540:intron 40.0%
AGTGGTGATATCGGAAATAC+AGG + chr1.2:62418288-62418307 None:intergenic 40.0%
CCTATTACCACAACTCAAGT+TGG - chr1.2:62418234-62418253 MS.gene07540:intron 40.0%
CGGCCTTTATTAAACATGGT+GGG - chr1.2:62417970-62417989 MS.gene07540:intron 40.0%
GATATCGGAAATACAGGAAG+CGG + chr1.2:62418108-62418127 None:intergenic 40.0%
GCAATTCAGGAAGAGAAAGA+GGG - chr1.2:62416782-62416801 MS.gene07540:intron 40.0%
GCTCAAGTCTTTCCTTTCAT+GGG - chr1.2:62418378-62418397 MS.gene07540:CDS 40.0%
GGTGGTAATAGTGGTGATAT+CGG + chr1.2:62418297-62418316 None:intergenic 40.0%
GTCATAGGAAATGGTGGTAA+TGG + chr1.2:62418135-62418154 None:intergenic 40.0%
GTTGTGGTAATAGGAAATGG+TGG + chr1.2:62418228-62418247 None:intergenic 40.0%
TACGGATGCAAAGAGATCAA+AGG - chr1.2:62416918-62416937 MS.gene07540:CDS 40.0%
TAGCCATATTGGTGAAAAGG+GGG + chr1.2:62417907-62417926 None:intergenic 40.0%
TCAAGTTTCAGATACACCTG+CGG - chr1.2:62416571-62416590 MS.gene07540:CDS 40.0%
TCACATTGTACCATATGGTG+GGG - chr1.2:62418320-62418339 MS.gene07540:CDS 40.0%
TCTATATATACACCAAGGCC+AGG + chr1.2:62417037-62417056 None:intergenic 40.0%
TGAAGAAAATCCCCACCATA+TGG + chr1.2:62418333-62418352 None:intergenic 40.0%
TGACGGTTTGACGAAAACAT+GGG - chr1.2:62417203-62417222 MS.gene07540:intron 40.0%
TGAGTTGCAGTCATAGGAAA+TGG + chr1.2:62418144-62418163 None:intergenic 40.0%
TGCAATTCAGGAAGAGAAAG+AGG - chr1.2:62416781-62416800 MS.gene07540:intron 40.0%
TGCTCAAGTCTTTCCTTTCA+TGG - chr1.2:62418377-62418396 MS.gene07540:CDS 40.0%
TGTTCACTTATGGGTCTGTA+AGG + chr1.2:62418012-62418031 None:intergenic 40.0%
TTGACGGTTTGACGAAAACA+TGG - chr1.2:62417202-62417221 MS.gene07540:intron 40.0%
TTGGTTGCTCTGCTAAACAA+AGG - chr1.2:62415107-62415126 MS.gene07540:CDS 40.0%
! ATGCTGAAATTCTAAGGGCA+AGG - chr1.2:62416456-62416475 MS.gene07540:intron 40.0%
! ATTAGGTCCAACTTGAGTTG+TGG + chr1.2:62418244-62418263 None:intergenic 40.0%
! CAGGTTGGACCAAATATCAA+CGG - chr1.2:62418075-62418094 MS.gene07540:intron 40.0%
! CCAACTTGAGTTGTGGTAAT+AGG + chr1.2:62418237-62418256 None:intergenic 40.0%
! GAAATTCTAAGGGCAAGGAA+AGG - chr1.2:62416461-62416480 MS.gene07540:intron 40.0%
! GCCTTGGTGTATATATAGAG+AGG - chr1.2:62417038-62417057 MS.gene07540:CDS 40.0%
! TTTTGCTCGACATCTGTCAT+GGG + chr1.2:62416837-62416856 None:intergenic 40.0%
! TTTTTGCTCGACATCTGTCA+TGG + chr1.2:62416838-62416857 None:intergenic 40.0%
!! ATAGGAAATGGTGGTAATGG+TGG + chr1.2:62418132-62418151 None:intergenic 40.0%
!! ATATGGTGGGGATTTTCTTC+AGG - chr1.2:62418332-62418351 MS.gene07540:CDS 40.0%
!! GAAAATCAAGTATCGAGCAC+TGG - chr1.2:62415082-62415101 MS.gene07540:CDS 40.0%
!! TTGCTGCAATATGATGATGG+TGG - chr1.2:62416626-62416645 MS.gene07540:CDS 40.0%
!! TTTCTTCAGGTTGGTGGAAA+TGG - chr1.2:62418345-62418364 MS.gene07540:CDS 40.0%
ACCTCGGAATAACCAGAAGA+AGG + chr1.2:62415169-62415188 None:intergenic 45.0%
AGCCATATTGGTGAAAAGGG+GGG + chr1.2:62417906-62417925 None:intergenic 45.0%
CAGCAACATCATTCCTCCAA+TGG + chr1.2:62416612-62416631 None:intergenic 45.0%
CCTATGACTGCAACTCAAGT+TGG - chr1.2:62418147-62418166 MS.gene07540:intron 45.0%
GACGCGGCCTTTATTAAACA+TGG - chr1.2:62417966-62417985 MS.gene07540:intron 45.0%
GATGATGAAGCAACTGCCAT+TGG - chr1.2:62416593-62416612 MS.gene07540:CDS 45.0%
GCGGCCTTTATTAAACATGG+TGG - chr1.2:62417969-62417988 MS.gene07540:intron 45.0%
GGAAATACAGGAAGCGGAAT+TGG + chr1.2:62418102-62418121 None:intergenic 45.0%
GGAATTGGCCCGTTGATATT+TGG + chr1.2:62418087-62418106 None:intergenic 45.0%
GGGGATGAGAAATTCAACAC+TGG + chr1.2:62416359-62416378 None:intergenic 45.0%
GGTGGTGATATCGGAAATAC+AGG + chr1.2:62418114-62418133 None:intergenic 45.0%
GGTTATTGTTGGCGCAAGTA+CGG - chr1.2:62416900-62416919 MS.gene07540:CDS 45.0%
GTTGCAGTCATAGGAAATGG+TGG + chr1.2:62418141-62418160 None:intergenic 45.0%
GTTTAATAAAGGCCGCGTCA+TGG + chr1.2:62417965-62417984 None:intergenic 45.0%
TAATAACCACACAAGCGGAG+AGG + chr1.2:62415838-62415857 None:intergenic 45.0%
TCGGAATAACCAGAAGAAGG+AGG + chr1.2:62415166-62415185 None:intergenic 45.0%
TTCTGCTGATGATTGCCATG+TGG - chr1.2:62416286-62416305 MS.gene07540:intron 45.0%
TTTGCTCGACATCTGTCATG+GGG + chr1.2:62416836-62416855 None:intergenic 45.0%
TTTGTCAATGACAGGTCCGT+GGG + chr1.2:62417776-62417795 None:intergenic 45.0%
! CCAACTTGAGTTGCAGTCAT+AGG + chr1.2:62418150-62418169 None:intergenic 45.0%
! CTAGTGGATCTTGTCCACAA+AGG - chr1.2:62416651-62416670 MS.gene07540:CDS 45.0%
! TCCTTCTTCTGGTTATTCCG+AGG - chr1.2:62415165-62415184 MS.gene07540:CDS 45.0%
! TTTTCATTCACTCCTGGCCT+TGG - chr1.2:62417022-62417041 MS.gene07540:CDS 45.0%
! TTTTGTCAATGACAGGTCCG+TGG + chr1.2:62417777-62417796 None:intergenic 45.0%
!! GGTGGTAATGGTGGTGATAT+CGG + chr1.2:62418123-62418142 None:intergenic 45.0%
!! TATGATGATGGTGGTGCTAG+TGG - chr1.2:62416635-62416654 MS.gene07540:CDS 45.0%
AAAGGCCGCGTCATGGTATT+AGG + chr1.2:62417958-62417977 None:intergenic 50.0%
ACACCACCTGATCAAGGACA+TGG - chr1.2:62416380-62416399 MS.gene07540:intron 50.0%
ATTGGTGAAAAGGGGGGAGA+GGG + chr1.2:62417900-62417919 None:intergenic 50.0%
CATCTCCTCTTGTTGCCACA+TGG + chr1.2:62416304-62416323 None:intergenic 50.0%
GATGAAGCAACTGCCATTGG+AGG - chr1.2:62416596-62416615 MS.gene07540:CDS 50.0%
GATTGCCATGTGGCAACAAG+AGG - chr1.2:62416296-62416315 MS.gene07540:intron 50.0%
GGGTCTGTAAGGTTGCATCA+TGG + chr1.2:62418001-62418020 None:intergenic 50.0%
GTCCTTGATCAGGTGGTGTT+GGG + chr1.2:62416379-62416398 None:intergenic 50.0%
GTCGTGTGTGCATGCATACA+CGG - chr1.2:62415571-62415590 MS.gene07540:CDS 50.0%
TATTGGTGAAAAGGGGGGAG+AGG + chr1.2:62417901-62417920 None:intergenic 50.0%
TCTGCACCATGTCCTTGATC+AGG + chr1.2:62416389-62416408 None:intergenic 50.0%
TGAACCATAGGCAATGCAGG+TGG + chr1.2:62417877-62417896 None:intergenic 50.0%
TGTCCTTGATCAGGTGGTGT+TGG + chr1.2:62416380-62416399 None:intergenic 50.0%
! CTCCCCCCTTTTCACCAATA+TGG - chr1.2:62417901-62417920 MS.gene07540:intron 50.0%
! GGTGGGGATTTTCTTCAGGT+TGG - chr1.2:62418336-62418355 MS.gene07540:CDS 50.0%
! GTTGGCCTAATACCATGACG+CGG - chr1.2:62417950-62417969 MS.gene07540:intron 50.0%
! TCCTTGATCAGGTGGTGTTG+GGG + chr1.2:62416378-62416397 None:intergenic 50.0%
! TTTTCACCTCTCCGCTTGTG+TGG - chr1.2:62415829-62415848 MS.gene07540:intron 50.0%
!! CAAGTATCGAGCACTGGAGT+TGG - chr1.2:62415088-62415107 MS.gene07540:CDS 50.0%
!!! GGGGATTTTCTTCAGGTTGG+TGG - chr1.2:62418339-62418358 MS.gene07540:CDS 50.0%
ACATCTGTCATGGGGCAAGG+TGG + chr1.2:62416828-62416847 None:intergenic 55.0%
ACCACCACCTGCATTGCCTA+TGG - chr1.2:62417870-62417889 MS.gene07540:intron 55.0%
ACCATAGGCAATGCAGGTGG+TGG + chr1.2:62417874-62417893 None:intergenic 55.0%
CCCAGCTGATCAGGAAGCTA+CGG - chr1.2:62418407-62418426 MS.gene07540:CDS 55.0%
CCGTAGCTTCCTGATCAGCT+GGG + chr1.2:62418410-62418429 None:intergenic 55.0%
CGTAGCTTCCTGATCAGCTG+GGG + chr1.2:62418409-62418428 None:intergenic 55.0%
GCACCATGTCCTTGATCAGG+TGG + chr1.2:62416386-62416405 None:intergenic 55.0%
GCAGTTGCTTCATCATCCGC+AGG + chr1.2:62416590-62416609 None:intergenic 55.0%
GGGTGAACCATAGGCAATGC+AGG + chr1.2:62417880-62417899 None:intergenic 55.0%
TCCCCAACACCACCTGATCA+AGG - chr1.2:62416374-62416393 MS.gene07540:intron 55.0%
TCCGTAGCTTCCTGATCAGC+TGG + chr1.2:62418411-62418430 None:intergenic 55.0%
TCGACATCTGTCATGGGGCA+AGG + chr1.2:62416831-62416850 None:intergenic 55.0%
!! AATGCAGGTGGTGGTGATGG+TGG + chr1.2:62417865-62417884 None:intergenic 55.0%
!! TGATGGTGGTGGCAGCATGT+TGG + chr1.2:62417851-62417870 None:intergenic 55.0%
TACACGGCCGTGGTTGCTAC+TGG - chr1.2:62415587-62415606 MS.gene07540:CDS 60.0%
TGTGCATGCATACACGGCCG+TGG - chr1.2:62415577-62415596 MS.gene07540:CDS 60.0%
! GGCAATGCAGGTGGTGGTGA+TGG + chr1.2:62417868-62417887 None:intergenic 60.0%
!! GCTGGGGAAGCACCCATGAA+AGG + chr1.2:62418393-62418412 None:intergenic 60.0%
GGGGGGAGAGGGTGAACCAT+AGG + chr1.2:62417889-62417908 None:intergenic 65.0%
GGGTGCTTCCCCAGCTGATC+AGG - chr1.2:62418398-62418417 MS.gene07540:CDS 65.0%
!! GCAGGTGGTGGTGATGGTGG+TGG + chr1.2:62417862-62417881 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr1.2 gene 62415025 62418449 62415025 ID=MS.gene07540
chr1.2 mRNA 62415025 62418449 62415025 ID=MS.gene07540.t1;Parent=MS.gene07540
chr1.2 exon 62418288 62418449 62418288 ID=MS.gene07540.t1.exon1;Parent=MS.gene07540.t1
chr1.2 CDS 62418288 62418449 62418288 ID=cds.MS.gene07540.t1;Parent=MS.gene07540.t1
chr1.2 exon 62416802 62417190 62416802 ID=MS.gene07540.t1.exon2;Parent=MS.gene07540.t1
chr1.2 CDS 62416802 62417190 62416802 ID=cds.MS.gene07540.t1;Parent=MS.gene07540.t1
chr1.2 exon 62416516 62416683 62416516 ID=MS.gene07540.t1.exon3;Parent=MS.gene07540.t1
chr1.2 CDS 62416516 62416683 62416516 ID=cds.MS.gene07540.t1;Parent=MS.gene07540.t1
chr1.2 exon 62415025 62415805 62415025 ID=MS.gene07540.t1.exon4;Parent=MS.gene07540.t1
chr1.2 CDS 62415025 62415805 62415025 ID=cds.MS.gene07540.t1;Parent=MS.gene07540.t1
Gene Sequence

>MS.gene07540

ATGTCTAATATCATGGAAAACAACAACACCAACACCAATAACAACAACAACAACAATGAAAATCAAGTATCGAGCACTGGAGTTGGTTGCTCTGCTAAACAAAGGAAGAGAAGAGATTTGATCGATTTAAATGAATCTCCTCCTTCTTCTGGTTATTCCGAGGTATTTATATACTCTTTTTACGCTAATACATGTAAAGATGATGATCAAGATCATGTATTTACAAAATATATATTGAGACAATCATATAATGATTAACTTTTAAAAAATATAAAAAATTTCATACAATTTTTATTTAGTTTATCTATACTATATGTAATATTAATTTTTGATCTAACATTTTTCTCTTTTAATTTTACACTTCAATTAAACTGTTTTGATCATAATTTTATCCTTTTTTATTTTTTGATGAAAATAAAACTAATGATTGATCATTTTAAATTAAATTGAAGCTCGATTTTTTTTTTTAAAGTTAAGCTCAGTTACTTGATGTCATTCATTGTTCAATTAAATAACTAATTTTTAAAATACGTATGTATACACATTGTCGTGTGTGCATGCATACACGGCCGTGGTTGCTACTGGTTCATTTTCATATAAATAAAAATGTAATACAAAGTAGATCTTCTTATTGAACTTTTTAAGCCGAATTTAATTGAAAAGCCATTTTAAATCTTGAATGAAGATGATAAAAAATTGTACCCTTTTTTTTTAAATAATGTCACTTTTTATTATTTACATTATATCAAAATATATTTTCACATTAATTATTATCCAATATAAACGAATCCAAATTCACATCATTTTTCACCTCTCCGCTTGTGTGGTTATTAAAAGTTCTCATTGCAATGCACCATTATTTCTCATTAGGAATATTTTTATTTTCCATTTTAGCACTAATACAACTTAGAACTTGAAACATTTAAACTCGACAAACATATTCCTAATCAGACCTTTAATTCAATCTAACATGTATTTATTATAATAATGTAATAAAATTATTATTCTTTATTTTCTTATCTAATTTTAATTATAATTTATTTTTGTATGTAAAAATATTTTTGTTTTTATAGTTTCATAGGCATATAATGCAATGCAAATAGTTTTACACCAACATGGAATGATTTATTTATTAGATTCAATTAATTTAAATTATGATACATATCATTTAAATTATAATTATTACATGAATGCTTATGAAATAATTAATTATGATTCACTAAAAATATACGTGTGTGTAATTGAATCAAATTCATTAGGCTTCTGCTGATGATTGCCATGTGGCAACAAGAGGAGATGCAAAACTTCAAGCTGAAACTGAAGTTCAACAACCAGTGTTGAATTTCTCATCCCCAACACCACCTGATCAAGGACATGGTGCAGAAGAAGAACAAGACCATGTGCAAAATGATGCAAATGTAGTGGTGAAAAATGCTGAAATTCTAAGGGCAAGGAAAGGGAAGATGCCAATGAACTTTTCTGAAGCAGTGCATGCCAAAGAAGATAGAACAACTGATGATAAAGAAACAAATGTACCAACTGATGATCAAGTTTCAGATACACCTGCGGATGATGAAGCAACTGCCATTGGAGGAATGATGTTGCTGCAATATGATGATGGTGGTGCTAGTGGATCTTGTCCACAAAGGTTTTATAATCACTTTGATAGTGTGAATATATATAGACACGCGTGCACACACACTTACATACATTGTTGCTAATAAATAAAGGTTAACTTTTTTTTGCTCAATTATTTTGCAATTCAGGAAGAGAAAGAGGGACTTCTCTGAATACTATAAACCACCTTGCCCCATGACAGATGTCGAGCAAAAAATAGTGATCCGAGTTGAAAGTGATGAAGACATTTTGGATGATGGTTATTGTTGGCGCAAGTACGGATGCAAAGAGATCAAAGGAAATCCTAACAATCGTAGGTAATTATTTACCTCTTTTATATGTGTTAATGCAAGTATGTGTATATTGATAAATGTTTTTCTTTTTCATTCACTCCTGGCCTTGGTGTATATATAGAGAGGAGGAAAATATGATGCAAGACATCATGCAATAGTACTTGAAAAATCTCTATAAAACCAATGATGATGAACACTGTTGTTGTTTAGAAGTAGAAGCTTACAATTCTCTTTATTTCCACAACTAAGCCAATGACATAATGATCATTGACGGTTTGACGAAAACATGGGAAATCATATATATGCTTTTCCTTGTTTTTGATATTTTTACAATGATAATTTTATTTTATCAAACTTTACAACATCACCGTGTTGAACCAACATCAATCATTGTTAGATTTAGAGATGATTTTCAAGTATTGGGCAAGAATTTATTTCTTCCGATAAGTGATTTATCGTAAAACTTGCCTAGAATTTGATATTAAATTTGTGGTTATACACACATGTTTATGTATATAATTTATCATTTTATTCATCAAACTTTATCATTTTATTTTAAGCGGCTTTCACTATAATTTTAATTATGCATGTGTGTGTGTTTTAAATAGTTACAAAGTTTCTTATCATTATCTAAACATTGTCCATTTACATAAATGTATTAGTATCAATGTATCATGCATCATATATATGAATATGTTTTGATTCCTAATTATTTAATATTGTGTGAATTGTAGGGGGTACTACAGATGCGCAACACACAATTGTGACATTAAAAAACAAGTGGAAAGGGATGCTAAAGATCCAAAATATGTACTTGTTACATATGATGGAAAACATACCCACGGACCTGTCATTGACAAAAAAAGACCAACATACTCGAGAAATATTAATGCATCTGGAGTTCGTAGAAATGCCAACATGCTGCCACCACCATCACCACCACCTGCATTGCCTATGGTTCACCCTCTCCCCCCTTTTCACCAATATGGCTATATACCTTATGGTATGAATTACGGTTGGCCTAATACCATGACGCGGCCTTTATTAAACATGGTGGGTAACACCATGATGCAACCTTACAGACCCATAAGTGAACAAAATTTTCTAGAACATCGTGCTTCAACTCCTATTGATACAAATCAGGTTGGACCAAATATCAACGGGCCAATTCCGCTTCCTGTATTTCCGATATCACCACCATTACCACCATTTCCTATGACTGCAACTCAAGTTGGACATAATATCAATGGGTCAGTTCCGCTTCCTGTATTTCCGATATCACCACCATTACCACCATTTCCTATTACCACAACTCAAGTTGGACCTAATATTAATGGGTCAATTTCACTTCCTGTATTTCCGATATCACCACTATTACCACCATTTCACATTGTACCATATGGTGGGGATTTTCTTCAGGTTGGTGGAAATGGTATTGTTGCTGCTCAAGTCTTTCCTTTCATGGGTGCTTCCCCAGCTGATCAGGAAGCTACGGATGTTCCTGACACCGAATGA
Protein sequence

>MS.gene07540.t1

MSNIMENNNTNTNNNNNNNENQVSSTGVGCSAKQRKRRDLIDLNESPPSSGYSEASADDCHVATRGDAKLQAETEVQQPVLNFSSPTPPDQGHGAEEEQDHVQNDANVVVKNAEILRARKGKMPMNFSEAVHAKEDRTTDDKETNVPTDDQVSDTPADDEATAIGGMMLLQYDDGGASGSCPQRKRKRDFSEYYKPPCPMTDVEQKIVIRVESDEDILDDGYCWRKYGCKEIKGNPNNRRGYYRCATHNCDIKKQVERDAKDPKYVLVTYDGKHTHGPVIDKKRPTYSRNINASGVRRNANMLPPPSPPPALPMVHPLPPFHQYGYIPYGMNYGWPNTMTRPLLNMVGNTMMQPYRPISEQNFLEHRASTPIDTNQVGPNINGPIPLPVFPISPPLPPFPMTATQVGHNINGSVPLPVFPISPPLPPFPITTTQVGPNINGSISLPVFPISPLLPPFHIVPYGGDFLQVGGNGIVAAQVFPFMGASPADQEATDVPDTE