Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07663.t1 | KEH38942.1 | 60.5 | 119 | 41 | 2 | 1 | 118 | 1 | 114 | 2.00E-24 | 122.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07663.t1 | Q8LJS2 | 44.4 | 126 | 59 | 2 | 1 | 120 | 1 | 121 | 2.6e-17 | 90.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07663.t1 | A0A072VB01 | 60.5 | 119 | 41 | 2 | 1 | 118 | 1 | 114 | 1.4e-24 | 122.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|---|---|
| MS.gene07663.t1 | TF | C2H2 |
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07663.t1 | MTR_2g084815 | 68.750 | 96 | 25 | 1 | 1 | 96 | 1 | 91 | 6.45e-38 | 131 |
| MS.gene07663.t1 | MTR_2g084815 | 56.338 | 71 | 22 | 3 | 87 | 148 | 209 | 279 | 3.93e-13 | 65.9 |
| MS.gene07663.t1 | MTR_2g084815 | 51.799 | 139 | 58 | 2 | 1 | 139 | 1 | 130 | 1.94e-36 | 127 |
| MS.gene07663.t1 | MTR_2g084815 | 52.206 | 136 | 56 | 2 | 1 | 136 | 1 | 127 | 5.37e-36 | 125 |
| MS.gene07663.t1 | MTR_2g084815 | 59.130 | 115 | 38 | 2 | 1 | 115 | 1 | 106 | 7.09e-36 | 127 |
| MS.gene07663.t1 | MTR_2g084815 | 56.338 | 71 | 22 | 3 | 87 | 148 | 232 | 302 | 5.18e-13 | 65.9 |
| MS.gene07663.t1 | MTR_4g055440 | 45.631 | 103 | 51 | 1 | 1 | 103 | 5 | 102 | 4.18e-22 | 90.1 |
| MS.gene07663.t1 | MTR_4g055450 | 40.196 | 102 | 44 | 1 | 2 | 103 | 6 | 90 | 2.81e-16 | 75.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene07663.t1 | AT5G22650 | 39.000 | 100 | 52 | 2 | 1 | 100 | 1 | 91 | 3.67e-15 | 72.0 |
| MS.gene07663.t1 | AT5G22650 | 40.426 | 94 | 47 | 2 | 1 | 94 | 1 | 85 | 4.09e-15 | 71.6 |
| MS.gene07663.t1 | AT5G03740 | 36.000 | 100 | 56 | 2 | 1 | 100 | 1 | 92 | 1.42e-14 | 70.1 |
| MS.gene07663.t1 | AT3G44750 | 39.773 | 88 | 45 | 3 | 1 | 88 | 1 | 80 | 7.13e-12 | 62.0 |
| MS.gene07663.t1 | AT3G44750 | 39.773 | 88 | 45 | 3 | 1 | 88 | 1 | 80 | 7.35e-12 | 62.0 |
Find 41 sgRNAs with CRISPR-Local
Find 83 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CACCGTTTCCAATGGAGTTT+TGG | 0.250093 | 4.1:-42414985 | None:intergenic |
| AGGACATTCACCTCTGAAAA+TGG | 0.279756 | 4.1:-42413540 | MS.gene07663:CDS |
| AGGACATTCACCTCTGAAAA+TGG | 0.279756 | 4.1:-42413715 | MS.gene07663:CDS |
| GAAATAAACACTCGCAGTTT+TGG | 0.283107 | 4.1:+42414366 | None:intergenic |
| CAAACAACCACAGGCTCTTC+TGG | 0.322693 | 4.1:+42414499 | None:intergenic |
| CTACGGCAAGAGAGTCTTCT+AGG | 0.324879 | 4.1:+42413661 | None:intergenic |
| GAAGTTTCAGCATCATTGTT+AGG | 0.324985 | 4.1:+42413850 | None:intergenic |
| AAATAAAGGCATACCAGAAC+AGG | 0.333664 | 4.1:-42414527 | MS.gene07663:CDS |
| TGGTGGCCAGAAGATTGTTT+TGG | 0.334121 | 4.1:-42414467 | MS.gene07663:CDS |
| TGAATTAGGTGCCGAGGTTA+AGG | 0.371872 | 4.1:-42414734 | MS.gene07663:intron |
| TCTTCTGGTATGCCCTGTTC+TGG | 0.374091 | 4.1:+42414514 | None:intergenic |
| GCACTTGGTGCAGCAAATAA+AGG | 0.387943 | 4.1:-42414541 | MS.gene07663:CDS |
| TTGTTAGATTCTGAGAAAAC+TGG | 0.390338 | 4.1:-42413904 | MS.gene07663:intron |
| TTAGGACCTTTCTTAGAGTC+AGG | 0.425080 | 4.1:+42413868 | None:intergenic |
| AGGTTCCCAAAACAATCTTC+TGG | 0.434819 | 4.1:+42414461 | None:intergenic |
| GATGCTGAAACTTCCAAGTC+TGG | 0.441612 | 4.1:-42413841 | MS.gene07663:CDS |
| GCATATACCTGCTGCAACTA+CGG | 0.445435 | 4.1:+42413644 | None:intergenic |
| AGCTGCTATTCACATTTCTC+AGG | 0.449259 | 4.1:-42414671 | MS.gene07663:intron |
| GCAAGAGAGTCCTCCAGACT+TGG | 0.490554 | 4.1:+42413828 | None:intergenic |
| ACTTCACTTAGCAGCATGCT+TGG | 0.492494 | 4.1:+42413497 | None:intergenic |
| AAGCATACCTCTTGCAACTA+CGG | 0.494531 | 4.1:+42413806 | None:intergenic |
| GTGAATAGCAGCTGCATCAT+GGG | 0.513124 | 4.1:+42414681 | None:intergenic |
| TCTCTTGCCGTAGTTGCAGC+AGG | 0.514413 | 4.1:-42413651 | MS.gene07663:intron |
| GCAGCTGCATCATGGGCATA+CGG | 0.515322 | 4.1:+42414688 | None:intergenic |
| AAAACGAGGCAAACAACCAC+AGG | 0.520382 | 4.1:+42414490 | None:intergenic |
| TGTGAATAGCAGCTGCATCA+TGG | 0.520591 | 4.1:+42414680 | None:intergenic |
| GCAATTCCTGACTCTAAGAA+AGG | 0.524303 | 4.1:-42413874 | MS.gene07663:CDS |
| GAGTCTTCTAGGAGCATGCT+TGG | 0.529371 | 4.1:+42413672 | None:intergenic |
| TGGATTGCAAGAACATATGA+AGG | 0.530216 | 4.1:-42413520 | MS.gene07663:CDS |
| TGGATTGCAAGAACATATGA+AGG | 0.530216 | 4.1:-42413695 | MS.gene07663:CDS |
| CAGCTGCATCATGGGCATAC+GGG | 0.543400 | 4.1:+42414689 | None:intergenic |
| GTTACTTTATAGGTAGCACT+TGG | 0.555076 | 4.1:-42414556 | MS.gene07663:intron |
| TTAGGTGCCGAGGTTAAGGT+TGG | 0.557285 | 4.1:-42414730 | MS.gene07663:intron |
| AGGTTACTCCAAAACTCCAT+TGG | 0.572648 | 4.1:+42414977 | None:intergenic |
| TCTCTTGCCGTAGTTGCAAG+AGG | 0.628928 | 4.1:-42413813 | MS.gene07663:intron |
| AGTTTCTCCAACCTTAACCT+CGG | 0.633677 | 4.1:+42414723 | None:intergenic |
| ATAGTATTAAAAGTTATCTG+AGG | 0.642805 | 4.1:+42414421 | None:intergenic |
| GATCTTTGAATTAGGTGCCG+AGG | 0.658816 | 4.1:-42414740 | MS.gene07663:intron |
| GCTGAAACTTCCAAGTCTGG+AGG | 0.660898 | 4.1:-42413838 | MS.gene07663:CDS |
| AATAAAGGCATACCAGAACA+GGG | 0.687887 | 4.1:-42414526 | MS.gene07663:CDS |
| GGGCATACCAGAAGAGCCTG+TGG | 0.690748 | 4.1:-42414506 | MS.gene07663:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATATATTTATTACTTTTAGA+TGG | - | chr4.1:42414872-42414891 | MS.gene07663:intron | 10.0% |
| !!! | TAATAGTTTAAATTTTACAT+TGG | - | chr4.1:42414380-42414399 | MS.gene07663:CDS | 10.0% |
| !!! | TATATTTATTACTTTTAGAT+GGG | - | chr4.1:42414873-42414892 | MS.gene07663:intron | 10.0% |
| !! | AAAGTAATAAATATATGCTT+CGG | + | chr4.1:42414704-42414723 | None:intergenic | 15.0% |
| !! | ATAATAATAAATAAGTCTCA+AGG | + | chr4.1:42414161-42414180 | None:intergenic | 15.0% |
| !! | TAAAGACAACAAAAAAATAA+AGG | + | chr4.1:42414734-42414753 | None:intergenic | 15.0% |
| !!! | TTTTAATACTATTCTTGAAA+AGG | - | chr4.1:42414064-42414083 | MS.gene07663:intron | 15.0% |
| !! | AAGTAATAAATATATGCTTC+AGG | + | chr4.1:42414703-42414722 | None:intergenic | 20.0% |
| !! | AAGTAATAAATATATGCTTC+GGG | + | chr4.1:42414703-42414722 | None:intergenic | 20.0% |
| !! | ATAGTATTAAAAGTTATCTG+AGG | + | chr4.1:42414056-42414075 | None:intergenic | 20.0% |
| !! | CAAGGATATATATCTATATA+TGG | + | chr4.1:42414519-42414538 | None:intergenic | 20.0% |
| !! | TAAAGACAAGAAAAAAAGAA+AGG | + | chr4.1:42414909-42414928 | None:intergenic | 20.0% |
| !! | TAAATAAACTAAGAGTAGTT+CGG | + | chr4.1:42413611-42413630 | None:intergenic | 20.0% |
| !! | TAATAGCTTAACATGTATAT+TGG | - | chr4.1:42414251-42414270 | MS.gene07663:intron | 20.0% |
| ! | ATCTAAATATGTAGCTAATG+TGG | - | chr4.1:42414357-42414376 | MS.gene07663:intron | 25.0% |
| !! | AAAAGTGTAAGCACAAATTA+CGG | + | chr4.1:42414552-42414571 | None:intergenic | 25.0% |
| !! | TTTTTCTTGTCTTTATTACC+AGG | - | chr4.1:42414914-42414933 | MS.gene07663:intron | 25.0% |
| !!! | TTTTTGTTGTCTTTATTACC+AGG | - | chr4.1:42414739-42414758 | MS.gene07663:intron | 25.0% |
| AACAGATCTATAAGACACTT+AGG | + | chr4.1:42414337-42414356 | None:intergenic | 30.0% | |
| ATGATGAAAGGAGAAATATG+AGG | + | chr4.1:42413520-42413539 | None:intergenic | 30.0% | |
| CCAAAACACATCAATAGTTA+AGG | + | chr4.1:42414424-42414443 | None:intergenic | 30.0% | |
| GAAGAAAAGTAGATGATGAA+AGG | + | chr4.1:42413532-42413551 | None:intergenic | 30.0% | |
| TATTCACATTTCTCAGGTTA+GGG | - | chr4.1:42413809-42413828 | MS.gene07663:intron | 30.0% | |
| TGTACGTTGATCTTTGAATT+AGG | - | chr4.1:42413726-42413745 | MS.gene07663:intron | 30.0% | |
| ! | CCTTAACTATTGATGTGTTT+TGG | - | chr4.1:42414421-42414440 | MS.gene07663:CDS | 30.0% |
| ! | TTGTTAGATTCTGAGAAAAC+TGG | - | chr4.1:42414570-42414589 | MS.gene07663:intron | 30.0% |
| AATTACGGATGTGTAACACA+AGG | + | chr4.1:42414537-42414556 | None:intergenic | 35.0% | |
| CTATTCACATTTCTCAGGTT+AGG | - | chr4.1:42413808-42413827 | MS.gene07663:intron | 35.0% | |
| GAAGTTTCAGCATCATTGTT+AGG | + | chr4.1:42414627-42414646 | None:intergenic | 35.0% | |
| TGGATTGCAAGAACATATGA+AGG | - | chr4.1:42414779-42414798 | MS.gene07663:intron | 35.0% | |
| TGTATGTACGTACGTGTATT+AGG | - | chr4.1:42413682-42413701 | MS.gene07663:CDS | 35.0% | |
| ! | AAATAAAGGCATACCAGAAC+AGG | - | chr4.1:42413947-42413966 | MS.gene07663:intron | 35.0% |
| ! | AATAAAGGCATACCAGAACA+GGG | - | chr4.1:42413948-42413967 | MS.gene07663:intron | 35.0% |
| ! | GAAATAAACACTCGCAGTTT+TGG | + | chr4.1:42414111-42414130 | None:intergenic | 35.0% |
| ! | GGTTTACTAAATTGCTGCTT+TGG | - | chr4.1:42413896-42413915 | MS.gene07663:CDS | 35.0% |
| ! | GTTACTTTATAGGTAGCACT+TGG | - | chr4.1:42413918-42413937 | MS.gene07663:intron | 35.0% |
| ! | TTCTTGCAATCCATTTTCAG+AGG | + | chr4.1:42414772-42414791 | None:intergenic | 35.0% |
| !! | ACGTTGTGTGTTTTTGTCTA+GGG | - | chr4.1:42413875-42413894 | MS.gene07663:CDS | 35.0% |
| !! | TACGTTGTGTGTTTTTGTCT+AGG | - | chr4.1:42413874-42413893 | MS.gene07663:CDS | 35.0% |
| !! | TGCTGCTTTGGTTACTTTAT+AGG | - | chr4.1:42413908-42413927 | MS.gene07663:intron | 35.0% |
| AAGCATACCTCTTGCAACTA+CGG | + | chr4.1:42414671-42414690 | None:intergenic | 40.0% | |
| AGCTGCTATTCACATTTCTC+AGG | - | chr4.1:42413803-42413822 | MS.gene07663:intron | 40.0% | |
| AGGTTCCCAAAACAATCTTC+TGG | + | chr4.1:42414016-42414035 | None:intergenic | 40.0% | |
| AGTTTCTCCAACCTTAACCT+CGG | + | chr4.1:42413754-42413773 | None:intergenic | 40.0% | |
| TTAGGACCTTTCTTAGAGTC+AGG | + | chr4.1:42414609-42414628 | None:intergenic | 40.0% | |
| ! | AGGAATTTTGTCCCTGACTA+AGG | + | chr4.1:42414036-42414055 | None:intergenic | 40.0% |
| ! | AGGACATTCACCTCTGAAAA+TGG | - | chr4.1:42414759-42414778 | MS.gene07663:intron | 40.0% |
| ! | ATTTTCAGAGGTGAATGTCC+TGG | + | chr4.1:42414760-42414779 | None:intergenic | 40.0% |
| ! | GCAATTCCTGACTCTAAGAA+AGG | - | chr4.1:42414600-42414619 | MS.gene07663:intron | 40.0% |
| !! | ACTGCGAGTGTTTATTTCTG+TGG | - | chr4.1:42414113-42414132 | MS.gene07663:intron | 40.0% |
| !!! | TGTTTTGGGAACCTTAGTCA+GGG | - | chr4.1:42414022-42414041 | MS.gene07663:intron | 40.0% |
| !!! | TTGTTTTGGGAACCTTAGTC+AGG | - | chr4.1:42414021-42414040 | MS.gene07663:intron | 40.0% |
| AAAACGAGGCAAACAACCAC+AGG | + | chr4.1:42413987-42414006 | None:intergenic | 45.0% | |
| GATGCTGAAACTTCCAAGTC+TGG | - | chr4.1:42414633-42414652 | MS.gene07663:intron | 45.0% | |
| GCATATACCTGCTGCAACTA+CGG | + | chr4.1:42414833-42414852 | None:intergenic | 45.0% | |
| GTGAATAGCAGCTGCATCAT+GGG | + | chr4.1:42413796-42413815 | None:intergenic | 45.0% | |
| TGTGAATAGCAGCTGCATCA+TGG | + | chr4.1:42413797-42413816 | None:intergenic | 45.0% | |
| ! | GATCTTTGAATTAGGTGCCG+AGG | - | chr4.1:42413734-42413753 | MS.gene07663:intron | 45.0% |
| ! | GGTGGCCAGAAGATTGTTTT+GGG | - | chr4.1:42414008-42414027 | MS.gene07663:intron | 45.0% |
| ! | GTGGTTGTTTGCCTCGTTTT+TGG | - | chr4.1:42413987-42414006 | MS.gene07663:intron | 45.0% |
| ! | TGGTGGCCAGAAGATTGTTT+TGG | - | chr4.1:42414007-42414026 | MS.gene07663:intron | 45.0% |
| !! | GCACTTGGTGCAGCAAATAA+AGG | - | chr4.1:42413933-42413952 | MS.gene07663:intron | 45.0% |
| !! | GGTGAGGCTCTGAAATATTG+AGG | - | chr4.1:42414473-42414492 | MS.gene07663:CDS | 45.0% |
| !! | GTTGTTTGCCTCGTTTTTGG+TGG | - | chr4.1:42413990-42414009 | MS.gene07663:intron | 45.0% |
| !! | TGAATTAGGTGCCGAGGTTA+AGG | - | chr4.1:42413740-42413759 | MS.gene07663:intron | 45.0% |
| AGCGAGGAAGAAGGTGAAAC+TGG | - | chr4.1:42414452-42414471 | MS.gene07663:CDS | 50.0% | |
| CAAACAACCACAGGCTCTTC+TGG | + | chr4.1:42413978-42413997 | None:intergenic | 50.0% | |
| CTACGGCAAGAGAGTCTTCT+AGG | + | chr4.1:42414816-42414835 | None:intergenic | 50.0% | |
| GCTGAAACTTCCAAGTCTGG+AGG | - | chr4.1:42414636-42414655 | MS.gene07663:intron | 50.0% | |
| GGAAGAAGGTGAAACTGGTG+AGG | - | chr4.1:42414457-42414476 | MS.gene07663:CDS | 50.0% | |
| TCTCTTGCCGTAGTTGCAAG+AGG | - | chr4.1:42414661-42414680 | MS.gene07663:intron | 50.0% | |
| TCTTCTGGCCACCAAAAACG+AGG | + | chr4.1:42414001-42414020 | None:intergenic | 50.0% | |
| TCTTCTGGTATGCCCTGTTC+TGG | + | chr4.1:42413963-42413982 | None:intergenic | 50.0% | |
| ! | GAGTCTTCTAGGAGCATGCT+TGG | + | chr4.1:42414805-42414824 | None:intergenic | 50.0% |
| !! | TTAGGTGCCGAGGTTAAGGT+TGG | - | chr4.1:42413744-42413763 | MS.gene07663:intron | 50.0% |
| CAGCTGCATCATGGGCATAC+GGG | + | chr4.1:42413788-42413807 | None:intergenic | 55.0% | |
| GCAAGAGAGTCCTCCAGACT+TGG | + | chr4.1:42414649-42414668 | None:intergenic | 55.0% | |
| GCAGCTGCATCATGGGCATA+CGG | + | chr4.1:42413789-42413808 | None:intergenic | 55.0% | |
| TCTCTTGCCGTAGTTGCAGC+AGG | - | chr4.1:42414823-42414842 | MS.gene07663:intron | 55.0% | |
| ACCTTCTTCCTCGCTCGTGC+AGG | + | chr4.1:42414447-42414466 | None:intergenic | 60.0% | |
| GGGCATACCAGAAGAGCCTG+TGG | - | chr4.1:42413968-42413987 | MS.gene07663:intron | 60.0% | |
| !!! | TGTTTTGGCCTGCACGAGCG+AGG | - | chr4.1:42414436-42414455 | MS.gene07663:CDS | 60.0% |
| GCCTGCACGAGCGAGGAAGA+AGG | - | chr4.1:42414443-42414462 | MS.gene07663:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 42413500 | 42414996 | 42413500 | ID=MS.gene07663 |
| chr4.1 | mRNA | 42413500 | 42414996 | 42413500 | ID=MS.gene07663.t1;Parent=MS.gene07663 |
| chr4.1 | exon | 42414984 | 42414996 | 42414984 | ID=MS.gene07663.t1.exon1;Parent=MS.gene07663.t1 |
| chr4.1 | CDS | 42414984 | 42414996 | 42414984 | ID=cds.MS.gene07663.t1;Parent=MS.gene07663.t1 |
| chr4.1 | exon | 42414672 | 42414748 | 42414672 | ID=MS.gene07663.t1.exon2;Parent=MS.gene07663.t1 |
| chr4.1 | CDS | 42414672 | 42414748 | 42414672 | ID=cds.MS.gene07663.t1;Parent=MS.gene07663.t1 |
| chr4.1 | exon | 42414377 | 42414566 | 42414377 | ID=MS.gene07663.t1.exon3;Parent=MS.gene07663.t1 |
| chr4.1 | CDS | 42414377 | 42414566 | 42414377 | ID=cds.MS.gene07663.t1;Parent=MS.gene07663.t1 |
| chr4.1 | exon | 42413814 | 42413919 | 42413814 | ID=MS.gene07663.t1.exon4;Parent=MS.gene07663.t1 |
| chr4.1 | CDS | 42413814 | 42413919 | 42413814 | ID=cds.MS.gene07663.t1;Parent=MS.gene07663.t1 |
| chr4.1 | exon | 42413652 | 42413735 | 42413652 | ID=MS.gene07663.t1.exon5;Parent=MS.gene07663.t1 |
| chr4.1 | CDS | 42413652 | 42413735 | 42413652 | ID=cds.MS.gene07663.t1;Parent=MS.gene07663.t1 |
| chr4.1 | exon | 42413500 | 42413560 | 42413500 | ID=MS.gene07663.t1.exon6;Parent=MS.gene07663.t1 |
| chr4.1 | CDS | 42413500 | 42413560 | 42413500 | ID=cds.MS.gene07663.t1;Parent=MS.gene07663.t1 |
| Gene Sequence |
| Protein sequence |
